Improving the soil and biomass with coal is estimated at the international level as a way to enhance soil productiveness, fertility and also to mitigate climate change. Biochar employed to improve land scope and impou...Improving the soil and biomass with coal is estimated at the international level as a way to enhance soil productiveness, fertility and also to mitigate climate change. Biochar employed to improve land scope and impound carbon, is attracting a great deal of attention. Its characteristics of chemical, physical and biological properties, containing big surface area, CEC (Cation Exchange Capacity), high water-holding capacity, size of pore, volume, distribution, and element composition, affect its recognized influences, particularly on microbial communities. These are discovered in the agriculture lands, lands remediation and composting. However, incomplete information existed about biochar for several farmers or peasants in agriculture scope. Therefore, farmers or peasants and gardeners are facing new opportunities and defiance each day, from feeding global extending and expanding population, whilst meeting severe new emissions requirements, to create more food on fewer land area while reducing their environmental emissions. Widespread application and utilization of biochar in agricultural scope, forestry production, energy, environmental protection and additional areas, has interested awareness by scientists and investigators inside and/or outside the country. The objective of this paper is to provide a guide for the farmers or peasants and gardeners with an essential information about biochar and what the ability of biochar can be achieved in the soil, and which can provide the scientific reference for the biochar application, and to get high yield and good quality of crops in all of different soils.展开更多
Rain-fed agriculture depends on the groundwater as a supplementary source of irrigation. The poor quality of water from the hard rock area is applied to the crops to save the crop. Continuous irrigation leads to degra...Rain-fed agriculture depends on the groundwater as a supplementary source of irrigation. The poor quality of water from the hard rock area is applied to the crops to save the crop. Continuous irrigation leads to degradation of soil, drip irrigation system as well plants. This study assessed the damages on the drip irrigation system and soil, inflicted by the use of low-quality irrigation water. The quality of water was improved with reference to raw water in terms of pH (1.57% - 5.88%), EC (3.08% - 10.08%), ions (0.96% - 46%) by using magnetization method, without disrupting the existing irrigation system in the basaltic aquifer in semi-arid to the arid condition. This was demonstrated before the farmers in central India.展开更多
Global food demand requires that soils be used intensively for agriculture, but how these soils are managed greatly impacts soil fluxes of carbon dioxide (CO2). Soil management practices can cause carbon to be either ...Global food demand requires that soils be used intensively for agriculture, but how these soils are managed greatly impacts soil fluxes of carbon dioxide (CO2). Soil management practices can cause carbon to be either sequestered or emitted, with corresponding uncertain influence on atmospheric CO2 concentrations. The situation is further complicated by the lack of CO2 flux measurements for African subsistence farms. For widespread application in remote areas, a simple experimental methodology is desired. As a first step, the present study investigated the use of Bowen Ratio Energy Balance (BREB) instrumentation to measure the energy balance and CO2 fluxes of two contrasting crop management systems, till and no-till, in the lowlands within the mountains of Lesotho. Two BREB micrometeorological systems were established on 100-m by 100-m sites, both planted with maize (Zea mays) but under either conventional (plow, disk-disk) or no-till soil mangement systems. The results demonstrate that with careful maintenance of the instruments by appropriately trained local personnel, the BREB approach offers substantial benefits in measuring real time changes in agroecosystem CO2 flux. The periods where the two treatments could be compared indicated greater CO2 sequestration over the no-till treatments during both the growing and non-growing seasons.展开更多
Chemical insecticides have been considered as a means to combat crop pests. Although their effectiveness is evident, their impact on the environment is increasingly being discussed. The aim of this study is to determi...Chemical insecticides have been considered as a means to combat crop pests. Although their effectiveness is evident, their impact on the environment is increasingly being discussed. The aim of this study is to determine the agro-ecological potential of a biological insecticide (C<sub>25</sub>H<sub>32</sub>O<sub>12</sub>) based on Aloe barbadensis in a Sahelian context. For this purpose, a completely randomized block experimental design with 3 replications and 4 treatments was set up to experiment with Aloe barbadensis as a bioinsecticide against pests of Abelmoschus esculentus. However, data were collected using an observation and parameter monitoring grid. This includes the cultivation of Abelmoschus esculentus, soil preparation, seeding and watering, plot labeling, preparation of the bioinsecticide (selection and preparation of raw materials, grinding of Aloe barbadensis miller and extraction of the crude bioinsecticide, quantification of treatment doses and dilution, and obtaining the formulated bioinsecticide), plant watering, plant treatment, and finally parameter monitoring. The results obtained reveal that the level of damage is significantly high in the control treatment T0 (63%) compared to the other treatments, with 29% for treatment T1, 7% for T2, and 1% for T3, implying a strong action capability of this insecticide against pests of Abelmoschus esculentus. Therefore, it can be concluded that for a normal growing season of Abelmoschus esculentus, this biological insecticide should be sprayed 12 times. Furthermore, this biological insecticide is unique in that it does not inflict any gastric toxicity on the pests, which gives it the characteristic of being a repellent. It is a biological insecticide whose efficacy period has been tested, with a minimum duration of 21 days. In conclusion, this formulated bioinsecticide based on Aloe barbadensis demonstrates significant efficacy against pests of Abelmoschus esculentus. In the future, we will consider experimenting with its effectiveness against pests of other plants.展开更多
Emerging new races of wheat stem rust(Puccinia graminis f.sp.tritici)are threatening global wheat(Triticum aestivum L.)production.Host resistance is the most effective and environmentally friendly method of controllin...Emerging new races of wheat stem rust(Puccinia graminis f.sp.tritici)are threatening global wheat(Triticum aestivum L.)production.Host resistance is the most effective and environmentally friendly method of controlling stem rust.The stem rust resistance gene Sr59 was previously identified within a T2DS 2RL wheat-rye whole arm translocation,providing broad-spectrum resistance to various stem rust races.Seedling evaluation,molecular marker analysis,and cytogenetic studies identified wheat-rye introgression line#284 containing a new translocation chromosome T2BL 2BS-2RL.This line has demonstrated broad-spectrum resistance to stem rust at the seedling stage.Seedling evaluation and cytogenetic analysis of three backcross populations between the line#284 and the adapted cultivars SLU-Elite,Navruz,and Linkert confirmed that Sr59 is located within the short distal 2RL translocation.This study aimed physical mapping of Sr59 in the 2RL introgression segment and develop a robust molecular marker for marker-assisted selection.Using genotyping-by-sequencing(GBS),GBS-derived SNPs were aligned with full-length annotated rye nucleotide-binding leucine-rich repeat(NLR)genes in the parental lines CS ph1b,SLU238,SLU-Elite,Navruz,and Linkert,as well as in 33 BC4F5progeny.Four NLR genes were identified on the 2R chromosome,with Chr2R_NLR_60 being tightly linked to the Sr59resistance gene.In-silico functional enrichment analysis of the translocated 2RL region(25,681,915 bp)identified 223 genes,with seven candidate genes associated with plant disease resistance and three linked to agronomic performance,contributing to oxidative stress response,protein kinase activity,and cellular homeostasis.These findings facilitate a better understanding of the genetic basis of stem rust resistance provided by Sr59.展开更多
Edible oils derived from aquatic products are rich in lipids beneficial to human health.However,the volatile flavor characteristics of flesh oil and liver oil from Doederleinia berycoides remain unclear.In this study,...Edible oils derived from aquatic products are rich in lipids beneficial to human health.However,the volatile flavor characteristics of flesh oil and liver oil from Doederleinia berycoides remain unclear.In this study,flesh oil and liver oil were extracted from Doederleinia berycoides,revealing different fatty acid compositions and contents.Lipidomics analysis identified a total of 124 differential lipids between the flesh oil and liver oil,including 42 glycerophospholipids(GPs),33 glycerolipids(GLs),23 free fatty acids(FAs),13 sphingolipids(SPs),10 sterols(STs),and 3 prenol lipids(PRs).Analysis using HS-GC-IMS identified 12 key volatile compounds that significantly contributed to the distinct volatile flavors of the flesh and liver oils.The volatile flavors originated from these volatile compounds,which had different Relative Odor Activity Values(ROAVs).Further results from HSSPME-GC-MS showed that the volatile flavors of the flesh oil and liver oil were respectively attributed to 64 and 35 volatile compounds,each with unique key volatile compounds exhibiting different ROAVs.There were significant positive or negative correlations between 18 key differential lipids and 24 volatile compounds in both flesh oil and liver oil.Therefore,the complex lipid profiles are responsible for the unique volatile flavors of flesh oil and liver oil,and the differential lipids play a central role in their volatile flavor formation.These findings provide a foundation for understanding the volatile flavor differences in fish oils and hold promise for further exploration of the molecular mechanisms underlying oil volatile flavors.展开更多
The increasing global population has led to a higher demand for food production, while a decrease in rural labor and a rise in production costs present complex challenges for the food industry. Smart agriculture is a ...The increasing global population has led to a higher demand for food production, while a decrease in rural labor and a rise in production costs present complex challenges for the food industry. Smart agriculture is a farm management concept that considers the deployment of Internet of Things (IoT) to address current food production challenges. In this regard, the agricultural sector is becoming increasingly data-focused, and requires data and technologies that are more precise, advanced, and cutting-edge than in the past. IoT enables agriculture to become data-driven, resulting in timely and more cost-effective farm intervention while reducing environmental impact. This review provides an analytical survey of the current and potential applications of IoT in smart agriculture to overcome challenges posed by spatio-temporal variability under varying environments and task diversity. This review also discusses the challenges that may arise from IoT deployment and presents an overview of the existing applications and those that may be developed in the future.展开更多
Fresh-cut lettuce is widely used in ready-to-eat salads sold in modified atmosphere packages (MAP).Even in MAP,fresh-cut lettuce has short shelf life that results in loss of nutrients.Lettuce cultivars exhibit genetic...Fresh-cut lettuce is widely used in ready-to-eat salads sold in modified atmosphere packages (MAP).Even in MAP,fresh-cut lettuce has short shelf life that results in loss of nutrients.Lettuce cultivars exhibit genetic variation for shelf life in MAP,but their variation for nutrient retention is not known.Fifty accessions were evaluated for initial content of ascorbic acid (AsA),carotenoids,and sugars and their retention in storage.Accessions with high content and/or good retention of one or more nutrients were identified.The romaine accession ‘Floricos’ had high levels of all the three nutrients.Accessions with relatively high retention of all the three nutrients were ‘Salinas 88’,‘Siskiyou’,‘Solar’,SM09A,‘Romance’,and ‘Green Towers’.Romaine cultivars,‘Balady Barrage’,‘Green Towers’,and ‘Darkland’ had relatively high initial levels of all tested nutrients and good rate of their retention.There was no clear correlation between initial AsA/carotene concentrations and their retention rates,suggesting that besides content,retention of nutrients should also be a breeding target in a lettuce nutritional improvement program.Statistical analyses with the Pearson's correlation coefficient determined a negative relationship between tissue deterioration(AUDePS) and retention of all tested nutrients[r of-0.52 (P<0.0001) for AsA,-0.27 (P<0.01) for total carotene,and-0.59 (P<0.0001) for total sugars],suggesting that an increase in tissue deterioration intensifies nutrient decay.Broad-sense heritability (H^(2)) across the experiments was0.15 for AsA,0.23 for total carotene,and 0.50 for total sugars.Identification of germplasm with high nutrient content,extended shelf life and good nutrient retention provides valuable information for the lettuce industry and associated breeding programs.展开更多
β-Aminobutyric acid(BABA)is a physiologically active plant compound that has not been extensively studied.It has been shown to increase resistance to biotic and abiotic stress factors and enhance seed germination in ...β-Aminobutyric acid(BABA)is a physiologically active plant compound that has not been extensively studied.It has been shown to increase resistance to biotic and abiotic stress factors and enhance seed germination in certain plant species.However,its effects on cereal grains with low germination rates have not yet been studied.This study investigated the effects of BABA on the germination of aged triticale seeds,the metabolite content of seedlings,and the state of their antioxidant systems.The study found that a three-hour treatment of seeds in BABA solutions at concentrations ranging from 0.1 to 1.0 mM increased germination energy and germination(by 10%–14%)and enhanced the accumulation of shoot and root biomass(by 17%–26%).Additionally,amylase activity increased in the grains,and the accumulation of osmolytes(sugars and proline)increased in the shoots.The content of anthocyanins in shoots increased by almost twofold,and the activity of antioxidant enzymes(superoxide dismutase,catalase,and guaiacol peroxidase)increased by approximately 20%–30%.Simultaneously,BABA seed priming caused a noticeable decrease in the levels of hydrogen peroxide and lipid peroxidation products in the shoots of seedlings.The conclusion was made that the use of BABA as a bioregulator has the potential to enhance the germination of seeds with low sowing qualities.This is due to the ability of BABA to activate the metabolism of reserve substances in the grain and prevent the development of oxidative stress.展开更多
The significance of gut microbiota(GM)in human health is being increasingly researched.An imbalance in GM composition,known as dysbiosis,is linked to various and other health issues.In addition,antibiotics are the pri...The significance of gut microbiota(GM)in human health is being increasingly researched.An imbalance in GM composition,known as dysbiosis,is linked to various and other health issues.In addition,antibiotics are the primary and most significant factors leading to major changes in the composition and function of the GM,which may result in colonization by antimicrobial-resistant(AMR)pathogens.Therefore,alternative antibiotic strategies for combating AMR pathogens are urgently needed.This narrative review highlights current knowledge regarding various pertinent strategies for decolonizing bacterial pathogens from GM and emphasizes decolonization therapies’critical role in pediatric surgical disorders.Strategies such as decontamination of the digestive tract utilizing antibiotics,the use of probiotics,and particularly fecal microbiota transplantation have introduced new options for clinical treatment.These treatments show the potential to restore GM balance and have demonstrated advantages for intestinal disorders related to pediatric surgery,including inflammatory bowel disease,neonatal necrotizing enterocolitis,Hirschsprung-associated enterocolitis,and short bowel syndrome.Despite GM therapeutics,recent strategies are still in their developmental phase and exhibit challenges that need further research.Thus,potential future directions for GMtargeted decolonization therapies are under consideration.Innovative alternative strategies to combat AMR though GM modulation in disorders related to pediatric surgery appear to be promising and should continue to be prioritized for further research and development.展开更多
Herbivorous insects and pathogens cause severe damage to rice tissues,affecting yield and grain quality.Damaged cells trigger downstream defense responses through various signals.Extracellular ATP(eATP),a signaling mo...Herbivorous insects and pathogens cause severe damage to rice tissues,affecting yield and grain quality.Damaged cells trigger downstream defense responses through various signals.Extracellular ATP(eATP),a signaling molecule released during mechanical cell damage,is considered a constitutive damage-associated molecular pattern(DAMP),which is crucial for initiating plant defense responses.Thus,understanding how rice plants cope with DAMPs such as eATP is essential.Here,we found that exogenous ATP affected rice growth and development,cell wall composition,chloroplast development,and cell death.Subsequent global transcriptome analysis revealed that several pathways were involved in the eATP response,including genes related to cell surface receptors,cell wall organization,chlorophyll biosynthesis,heat and temperature stimulation,epigenetic regulation,and reactive oxygen species metabolism.Cell surface receptors,including members of the lectin receptor-like kinases(LecRKs),were found to participate in the eATP response.We further investigated ATP-induced genes in T-DNA activation mutants of OsLecRKs,demonstrating their involvement in eATP signaling in rice.This study confirms a DAMP-mediated transcriptional response in plants and provides novel candidates for advancing resistant rice breeding against insect herbivores and pathogens.展开更多
Decision Support Tool(DST)enables farmers to make site-specific crop management decisions;however,comprehensive calibration can be both costly and time-consuming.This study assessed the production and economic benefit...Decision Support Tool(DST)enables farmers to make site-specific crop management decisions;however,comprehensive calibration can be both costly and time-consuming.This study assessed the production and economic benefits of two calibrations of the Nutrient Expert(NE)tool for rice in Sri Lanka’s Alfisols:the basic calibration(Nutrient Expert Sri Lanka 1,NESL1)and the comprehensive calibration(Nutrient Expert Sri Lanka 2,NESL2).NESL1 was developed by adapting the South Indian version of NE to local conditions,while NESL2 was an updated version,using three years of data from 71 farmer fields.展开更多
Houttuynia cordata, a characteristic edible and medicinal plant in southwestern China, is prone to absorbing lead (Pb^(2+)). Excessive consumption may lead to Pb^(2+) accumulation in the human body, which has been lin...Houttuynia cordata, a characteristic edible and medicinal plant in southwestern China, is prone to absorbing lead (Pb^(2+)). Excessive consumption may lead to Pb^(2+) accumulation in the human body, which has been linked to serious health risks such as neurotoxicity, kidney damage, anemia, and developmental disorders, particularly in children. Therefore, the development of molecular markers associated with Pb^(2+) uptake and the investigation of the plant’s physiological responses to Pb^(2+) pollution are of great significance. In this study, 72 H. cordata germplasms were evaluated for Pb^(2+) accumulation after exogenous Pb^(2+) treatment. A significant variation in Pb^(2+) content was observed among the germplasms, indicating rich genetic diversity. Using RAPD markers, seven loci were identified to be significantly associated with Pb^(2+) uptake, with locus 43 (R^(2) = 6.72%) and locus 53 (R^(2) = 5.39%) showing the strongest correlations. Marker validation was performed using five low- and five high-accumulating accessions. Two representative germplasms were further subjected to 0, 500 and 1000 mg/kg Pb^(2+) treatments for 40 days. Pb^(2+) content, membrane lipid peroxidation, and redox enzyme activities (SOD, POD and CAT) were measured across different organs. Organs with greater soil contact (roots) exhibited higher Pb^(2+) accumulation and oxidative damage. POD and CAT activities were markedly induced by Pb^(2+) stress, while SOD response was limited. This study provides a theoretical foundation for breeding low Pb^(2+)-accumulating H. cordata varieties through marker-assisted selection (MAS) and supports their safe use and application in phytoremediation.展开更多
Pinus densiflora is a pine species native to the Korean peninsula,and seed orchards have supplied mate-rial needed for afforestation in South Korea.Climate vari-ables affecting seed production have not been identified...Pinus densiflora is a pine species native to the Korean peninsula,and seed orchards have supplied mate-rial needed for afforestation in South Korea.Climate vari-ables affecting seed production have not been identified.The purpose of this study was to determine climate variables that influence annual seed production of two seed orchards using multiple linear regression(MLR),elastic net regres-sion(ENR)and partial least square regression(PLSR)mod-els.The PLSR model included 12 climatic variables from 2003 to 2020 and explained 74.3%of the total variation in seed production.It showed better predictive performance(R2=0.662)than the EN(0.516)and the MLR(0.366)mod-els.Among the 12 climatic variables,July temperature two years prior to seed production and July precipitation after one year had the strongest influence on seed production.The time periods indicated by the two variables corresponded to pollen cone initiation and female gametophyte development.The results will be helpful for developing seed collection plans,selecting new orchard sites with favorable climatic conditions,and investigating the relationships between seed production and climatic factors in related pine species.展开更多
Background The prolificacy of sows(litter size at birth)has markedly increased,leading to higher post-natal mor-tality.Heat stress can exacerbate this issue.Arginine plays an important role in several physiological pa...Background The prolificacy of sows(litter size at birth)has markedly increased,leading to higher post-natal mor-tality.Heat stress can exacerbate this issue.Arginine plays an important role in several physiological pathways;its effect on gestating sows can depend on the period of supplementation.This study evaluated the effects of arginine supplementation on the productive performance and physiological status of sows during different gestation periods and seasons,using a multi-omics approach.Methods A total of 320 sows were divided into 4 groups over 2 seasons(warm/cold);a control group(CO)received a standard diet(including 16.5 g/d of arginine)and 3 other groups received the standard diet supplemented with 21.8 g/d of arginine(38.3 g/d of arginine)either during the first 35 d(Early35),the last 45 d(Late45)or through-out the entire gestation period(COM).The colostrum was analyzed for nutritional composition,immunoglobulins and metabolomic profile.Urine and feces were analyzed on d 35 and 106 for the metabolomic and microbial profiles.Piglet body weight and mortality were recorded at birth,d 6,d 26,and on d 14 post-weaning.Results Interactions between arginine and season were never significant.The Early35 group had a lower percent-age of stillborn(P<0.001),mummified(P=0.002)and low birthweight(LBW)piglets(P=0.02)than the CO group.The Late45 group had a lower percentage of stillborn piglets(P=0.029)and a higher percentage of high birthweight piglets(HBW;P<0.001)than the CO group.The COM group had a higher percentage of LBW(P=0.004)and crushed piglets(P<0.001)than the CO group.Arginine supplementation modifies the metabolome characterization of colos-trum,urine,and feces.Creatine and nitric oxide pathways,as well as metabolites related to microbial activity,were influenced in all matrices.A slight trend in the beta diversity index was observed in the microbiome profile on d 35(P=0.064).Conclusions Arginine supplementation during early gestation reduced the percentage of stillborn and LBW piglets,while in the last third of pregnancy,it favored the percentage of HBW pigs and reduced the percentage of stillbirths,showing that arginine plays a significant role in the physiology of pregnant sows.展开更多
This study analyzes the causes and effects of climate change in the upper Niger River basin and the implementation of local adaptation strategies based on EMS(Environmental Management Systems).It aims to strengthen ec...This study analyzes the causes and effects of climate change in the upper Niger River basin and the implementation of local adaptation strategies based on EMS(Environmental Management Systems).It aims to strengthen ecological resilience and sustainable natural resource management practices through training,awareness-raising,and community participation.The work was conducted in three rural communes in the Kissidougou prefecture,located in the Faranah administrative region.Data collection and analysis tools included questionnaires,GPS devices,digital devices,laptops,and Excel and SPSS software.The methodology employed a participatory and multidisciplinary approach combining a literature review,surveys of 163 respondents,semi-structured interviews with 16 key informants,training for 218 technical staff and local elected officials(30%of whom were women),and awareness-raising activities for 1,800 participants in local languages.Five community forests covering 443.58 hectares were integrated into management plans,concerted,under the coordination of the NGO APARFE.The results show an increase in average temperature(+0.8°C since 1960),a decrease in rainfall(-5.3 mm/month),and increased vulnerability of populations dependent on agriculture.The integration of the EMS(Environmental Management System)has led to improvements in environmental governance,community forest management,awareness of sustainable agricultural practices,and the inclusion of women(51%of participants).In short,the EMS is an effective tool for strengthening community resilience and environmental sustainability.展开更多
Nitrogen(N)and phosphorus(P)are mineral nutrients essential for plant growth and development,playing a crucial role throughout the plant life cycle.Cotton,a globally significant textile crop,has a particularly high de...Nitrogen(N)and phosphorus(P)are mineral nutrients essential for plant growth and development,playing a crucial role throughout the plant life cycle.Cotton,a globally significant textile crop,has a particularly high demand for N fertilizer across its developmental stages.This review explores the effects of adequate or deficient N and P levels on cotton growth phases,focusing on their influence on physiological processes and molecular mechanisms.Key topics include the regulation of N-and P-related enzymes,hormones,and genes,as well as the complex interplay of N-and P-related signaling pathways from the aspects of N-P signaling integration to regulate root development,N-P signaling integration to regulate nutrient uptake,and regulation of N-P interactions—a frontier in current research.Strategies for improving N and P use efficiency are also discussed,including developing high-efficiency cotton cultivars and identifying functional genes to enhance productivity.Generally speaking,we take model plants as a reference in the hope of coming up with new strategies for the efficient utilization of N and P in cotton.展开更多
This study aimed to explore the relationship between Soil-Plant Analysis Development(SPAD)values and key environmental factors in cucumber(Cucumis sativus L.)cultivation in a greenhouse.SPAD values,indicative of chlor...This study aimed to explore the relationship between Soil-Plant Analysis Development(SPAD)values and key environmental factors in cucumber(Cucumis sativus L.)cultivation in a greenhouse.SPAD values,indicative of chlorophyll content,reflect plant health and productivity.The analysis revealed strong positive correlations between SPADvalues and both indoor light intensity(ILI,r=0.59,p<0.001)and outdoor light intensity(OLI,r=0.62,p<0.001),suggesting that higher light intensities were associated with enhanced SPAD values.In contrast,significant negative correlations were found between SPAD values and soil temperature at 15-30 cm depth(ST1530,r=−0.47,p<0.001)and volumetric soil moisture content at the same depth(SM1530,r=−0.52,p<0.001),with higher soil temperatures(e.g.,28℃)and excessive moisture(e.g.,25%)leading to reduced SPAD values.Multiple regression analysis identified ST1530 and SM1530 as significant negative predictors of SPAD,with coefficients of−0.97(p=0.05)and−0.34(p=0.05),respectively,suggesting that increases in soil temperature and moisture result in lower SPAD values.Indoor light intensity(e.g.,600-800μmol/m^(2)/s)emerged as a significant positive contributor,with a coefficient of 0.01(p<0.001),highlighting its role in promoting chlorophyll synthesis.Additionally,relative humidity(r=0.27,p<0.01)showed a positive,although less pronounced,association with SPAD.These results underscore the importance of both direct and indirect environmental factors in influencing SPAD variability and,by extension,plant health and productivity in cucumber cultivation.展开更多
In order to contribute to a better understanding of the biodiversity of local chicken populations, this study focused on the description of the essential qualitative parameters in the phenotypic characterization of lo...In order to contribute to a better understanding of the biodiversity of local chicken populations, this study focused on the description of the essential qualitative parameters in the phenotypic characterization of local species. Conducted in 6 localities in the Far North Region of Cameroon (Doukoula, Yagoua, Guidiguis, Dziguilao, Maroua 3rd and Méri), a population of 240 local chickens, including 172 hens and 68 cocks were characterized in family farms. The choice of localities was made on the basis of their strong potential local chicken flocks in the region. To this end, each animal was the subject of a qualitative description based on the identification of the sex of the animal, the description of the colorations of the plumage and extremities, and the description of the types of format, plumage and crests. All observations were made with the naked eye and in daylight and then photographed. The main results show that the plumage colors are multiple and dominated by the White-Pied-Black (21.7%) and the Mille-fleur (20.8%);the wattles are dominated by the red (60%) and the pink (35.4%);the tarsi are dominated by the white (43.8%) and the black (32.08%);the white skin (92.5%) dominates over the pink skin (7.5%);the “Smooth-uniform” feather is dominant (97.08%), followed by the smooth-crested type (2.08%) and the fries type (0.82%);the medium size of the chickens is dominant (86.66%), followed by the dwarf size (9.58%) and the large size (3.75%). The results of this study demonstrate that there is a strong phenotypic diversity within the local chicken population. This diversity can serve as a basis for the development of selection, conservation and genetic potential improvement programs based on rational exploitation of the local chicken.展开更多
The escalating global dissemination of plasmid-mediated antibiotic resistance poses a formidable threat to global health.Conjugation stands as a pivotal mechanism for horizontal gene transfer among bacterial populatio...The escalating global dissemination of plasmid-mediated antibiotic resistance poses a formidable threat to global health.Conjugation stands as a pivotal mechanism for horizontal gene transfer among bacterial populations,facilitating the spread of antibiotic resistance genes(ARGs).Microelectrolysis has garnered attention as an efficacious strategy for mitigating antibiotic concentrations in wastewater,yet its potential impact on ARG horizontal transfer remain largely unexplored.This comprehensive investigation unveils that microelectrolysis not only influences but significantly accelerates the conjugative transfer of ARG-harboring plasmids.Remarkably,this phenomenon is corroborated at the microbial community scale,underscoring its ecological relevance.Alarmingly,the study highlights the vulnerability of intestinalmicroorganisms to acquire antibiotic resistance under electrolytic stimulation,posing heightened risks to both animal and human health.Delving deeper,the study elucidates the underlyingmechanisms responsible for this enhanced conjugative transfer.It reveals that microelectrolysis augments the abundance of mating-competent cells,triggers the generation of reactive oxygen species,inflicts modest membrane damage,and upregulates the expression of genes critical for conjugation.These findings collectively contribute to a more profound comprehension of the environmental dissemination dynamics and associated public health implications of ARGs in the context of wastewater treatment employing microelectrolytic technologies.展开更多
文摘Improving the soil and biomass with coal is estimated at the international level as a way to enhance soil productiveness, fertility and also to mitigate climate change. Biochar employed to improve land scope and impound carbon, is attracting a great deal of attention. Its characteristics of chemical, physical and biological properties, containing big surface area, CEC (Cation Exchange Capacity), high water-holding capacity, size of pore, volume, distribution, and element composition, affect its recognized influences, particularly on microbial communities. These are discovered in the agriculture lands, lands remediation and composting. However, incomplete information existed about biochar for several farmers or peasants in agriculture scope. Therefore, farmers or peasants and gardeners are facing new opportunities and defiance each day, from feeding global extending and expanding population, whilst meeting severe new emissions requirements, to create more food on fewer land area while reducing their environmental emissions. Widespread application and utilization of biochar in agricultural scope, forestry production, energy, environmental protection and additional areas, has interested awareness by scientists and investigators inside and/or outside the country. The objective of this paper is to provide a guide for the farmers or peasants and gardeners with an essential information about biochar and what the ability of biochar can be achieved in the soil, and which can provide the scientific reference for the biochar application, and to get high yield and good quality of crops in all of different soils.
文摘Rain-fed agriculture depends on the groundwater as a supplementary source of irrigation. The poor quality of water from the hard rock area is applied to the crops to save the crop. Continuous irrigation leads to degradation of soil, drip irrigation system as well plants. This study assessed the damages on the drip irrigation system and soil, inflicted by the use of low-quality irrigation water. The quality of water was improved with reference to raw water in terms of pH (1.57% - 5.88%), EC (3.08% - 10.08%), ions (0.96% - 46%) by using magnetization method, without disrupting the existing irrigation system in the basaltic aquifer in semi-arid to the arid condition. This was demonstrated before the farmers in central India.
文摘Global food demand requires that soils be used intensively for agriculture, but how these soils are managed greatly impacts soil fluxes of carbon dioxide (CO2). Soil management practices can cause carbon to be either sequestered or emitted, with corresponding uncertain influence on atmospheric CO2 concentrations. The situation is further complicated by the lack of CO2 flux measurements for African subsistence farms. For widespread application in remote areas, a simple experimental methodology is desired. As a first step, the present study investigated the use of Bowen Ratio Energy Balance (BREB) instrumentation to measure the energy balance and CO2 fluxes of two contrasting crop management systems, till and no-till, in the lowlands within the mountains of Lesotho. Two BREB micrometeorological systems were established on 100-m by 100-m sites, both planted with maize (Zea mays) but under either conventional (plow, disk-disk) or no-till soil mangement systems. The results demonstrate that with careful maintenance of the instruments by appropriately trained local personnel, the BREB approach offers substantial benefits in measuring real time changes in agroecosystem CO2 flux. The periods where the two treatments could be compared indicated greater CO2 sequestration over the no-till treatments during both the growing and non-growing seasons.
文摘Chemical insecticides have been considered as a means to combat crop pests. Although their effectiveness is evident, their impact on the environment is increasingly being discussed. The aim of this study is to determine the agro-ecological potential of a biological insecticide (C<sub>25</sub>H<sub>32</sub>O<sub>12</sub>) based on Aloe barbadensis in a Sahelian context. For this purpose, a completely randomized block experimental design with 3 replications and 4 treatments was set up to experiment with Aloe barbadensis as a bioinsecticide against pests of Abelmoschus esculentus. However, data were collected using an observation and parameter monitoring grid. This includes the cultivation of Abelmoschus esculentus, soil preparation, seeding and watering, plot labeling, preparation of the bioinsecticide (selection and preparation of raw materials, grinding of Aloe barbadensis miller and extraction of the crude bioinsecticide, quantification of treatment doses and dilution, and obtaining the formulated bioinsecticide), plant watering, plant treatment, and finally parameter monitoring. The results obtained reveal that the level of damage is significantly high in the control treatment T0 (63%) compared to the other treatments, with 29% for treatment T1, 7% for T2, and 1% for T3, implying a strong action capability of this insecticide against pests of Abelmoschus esculentus. Therefore, it can be concluded that for a normal growing season of Abelmoschus esculentus, this biological insecticide should be sprayed 12 times. Furthermore, this biological insecticide is unique in that it does not inflict any gastric toxicity on the pests, which gives it the characteristic of being a repellent. It is a biological insecticide whose efficacy period has been tested, with a minimum duration of 21 days. In conclusion, this formulated bioinsecticide based on Aloe barbadensis demonstrates significant efficacy against pests of Abelmoschus esculentus. In the future, we will consider experimenting with its effectiveness against pests of other plants.
基金the financial support from FORMAS(2018-01029)the Swedish Institute(01132-2022)for supporting Ivan Motsnyi’s visit and research at Swedish University of Agricultural Sciences。
文摘Emerging new races of wheat stem rust(Puccinia graminis f.sp.tritici)are threatening global wheat(Triticum aestivum L.)production.Host resistance is the most effective and environmentally friendly method of controlling stem rust.The stem rust resistance gene Sr59 was previously identified within a T2DS 2RL wheat-rye whole arm translocation,providing broad-spectrum resistance to various stem rust races.Seedling evaluation,molecular marker analysis,and cytogenetic studies identified wheat-rye introgression line#284 containing a new translocation chromosome T2BL 2BS-2RL.This line has demonstrated broad-spectrum resistance to stem rust at the seedling stage.Seedling evaluation and cytogenetic analysis of three backcross populations between the line#284 and the adapted cultivars SLU-Elite,Navruz,and Linkert confirmed that Sr59 is located within the short distal 2RL translocation.This study aimed physical mapping of Sr59 in the 2RL introgression segment and develop a robust molecular marker for marker-assisted selection.Using genotyping-by-sequencing(GBS),GBS-derived SNPs were aligned with full-length annotated rye nucleotide-binding leucine-rich repeat(NLR)genes in the parental lines CS ph1b,SLU238,SLU-Elite,Navruz,and Linkert,as well as in 33 BC4F5progeny.Four NLR genes were identified on the 2R chromosome,with Chr2R_NLR_60 being tightly linked to the Sr59resistance gene.In-silico functional enrichment analysis of the translocated 2RL region(25,681,915 bp)identified 223 genes,with seven candidate genes associated with plant disease resistance and three linked to agronomic performance,contributing to oxidative stress response,protein kinase activity,and cellular homeostasis.These findings facilitate a better understanding of the genetic basis of stem rust resistance provided by Sr59.
基金supported by the R&D Projects in Key Areas of Guangdong Province(2023B0202080003)the National Natural Science Foundation of China(32472272,32302135,32072291)+1 种基金“Pioneer”and“Leading Goose”R&D Program of Zhejiang(2023C02006)Youth S&T Talent Support Programme of Guangdong Provincial Association for Science and Technology(SKXRC202401)。
文摘Edible oils derived from aquatic products are rich in lipids beneficial to human health.However,the volatile flavor characteristics of flesh oil and liver oil from Doederleinia berycoides remain unclear.In this study,flesh oil and liver oil were extracted from Doederleinia berycoides,revealing different fatty acid compositions and contents.Lipidomics analysis identified a total of 124 differential lipids between the flesh oil and liver oil,including 42 glycerophospholipids(GPs),33 glycerolipids(GLs),23 free fatty acids(FAs),13 sphingolipids(SPs),10 sterols(STs),and 3 prenol lipids(PRs).Analysis using HS-GC-IMS identified 12 key volatile compounds that significantly contributed to the distinct volatile flavors of the flesh and liver oils.The volatile flavors originated from these volatile compounds,which had different Relative Odor Activity Values(ROAVs).Further results from HSSPME-GC-MS showed that the volatile flavors of the flesh oil and liver oil were respectively attributed to 64 and 35 volatile compounds,each with unique key volatile compounds exhibiting different ROAVs.There were significant positive or negative correlations between 18 key differential lipids and 24 volatile compounds in both flesh oil and liver oil.Therefore,the complex lipid profiles are responsible for the unique volatile flavors of flesh oil and liver oil,and the differential lipids play a central role in their volatile flavor formation.These findings provide a foundation for understanding the volatile flavor differences in fish oils and hold promise for further exploration of the molecular mechanisms underlying oil volatile flavors.
文摘The increasing global population has led to a higher demand for food production, while a decrease in rural labor and a rise in production costs present complex challenges for the food industry. Smart agriculture is a farm management concept that considers the deployment of Internet of Things (IoT) to address current food production challenges. In this regard, the agricultural sector is becoming increasingly data-focused, and requires data and technologies that are more precise, advanced, and cutting-edge than in the past. IoT enables agriculture to become data-driven, resulting in timely and more cost-effective farm intervention while reducing environmental impact. This review provides an analytical survey of the current and potential applications of IoT in smart agriculture to overcome challenges posed by spatio-temporal variability under varying environments and task diversity. This review also discusses the challenges that may arise from IoT deployment and presents an overview of the existing applications and those that may be developed in the future.
基金supported by the Specialty Crop Block Grant Program of the U.S. Department of Agriculture's (USDA), Agricultural Marketing Service (AMS) through the California Department of Food and Agriculture (SCB15015)partly also by funding from the California Leafy Greens Research Board and by Oak Ridge Institute for Science and Education (ORISE) Research Participation Program。
文摘Fresh-cut lettuce is widely used in ready-to-eat salads sold in modified atmosphere packages (MAP).Even in MAP,fresh-cut lettuce has short shelf life that results in loss of nutrients.Lettuce cultivars exhibit genetic variation for shelf life in MAP,but their variation for nutrient retention is not known.Fifty accessions were evaluated for initial content of ascorbic acid (AsA),carotenoids,and sugars and their retention in storage.Accessions with high content and/or good retention of one or more nutrients were identified.The romaine accession ‘Floricos’ had high levels of all the three nutrients.Accessions with relatively high retention of all the three nutrients were ‘Salinas 88’,‘Siskiyou’,‘Solar’,SM09A,‘Romance’,and ‘Green Towers’.Romaine cultivars,‘Balady Barrage’,‘Green Towers’,and ‘Darkland’ had relatively high initial levels of all tested nutrients and good rate of their retention.There was no clear correlation between initial AsA/carotene concentrations and their retention rates,suggesting that besides content,retention of nutrients should also be a breeding target in a lettuce nutritional improvement program.Statistical analyses with the Pearson's correlation coefficient determined a negative relationship between tissue deterioration(AUDePS) and retention of all tested nutrients[r of-0.52 (P<0.0001) for AsA,-0.27 (P<0.01) for total carotene,and-0.59 (P<0.0001) for total sugars],suggesting that an increase in tissue deterioration intensifies nutrient decay.Broad-sense heritability (H^(2)) across the experiments was0.15 for AsA,0.23 for total carotene,and 0.50 for total sugars.Identification of germplasm with high nutrient content,extended shelf life and good nutrient retention provides valuable information for the lettuce industry and associated breeding programs.
基金support from the project of the Ministry of Education and Science of Ukraine 2-24-26 BO Development of Measures to Ensure Sustainable Productivity of Agrophytocenoses under the Influence of Abiotic and Biotic Stress Factors,state registration No.0124U000457.
文摘β-Aminobutyric acid(BABA)is a physiologically active plant compound that has not been extensively studied.It has been shown to increase resistance to biotic and abiotic stress factors and enhance seed germination in certain plant species.However,its effects on cereal grains with low germination rates have not yet been studied.This study investigated the effects of BABA on the germination of aged triticale seeds,the metabolite content of seedlings,and the state of their antioxidant systems.The study found that a three-hour treatment of seeds in BABA solutions at concentrations ranging from 0.1 to 1.0 mM increased germination energy and germination(by 10%–14%)and enhanced the accumulation of shoot and root biomass(by 17%–26%).Additionally,amylase activity increased in the grains,and the accumulation of osmolytes(sugars and proline)increased in the shoots.The content of anthocyanins in shoots increased by almost twofold,and the activity of antioxidant enzymes(superoxide dismutase,catalase,and guaiacol peroxidase)increased by approximately 20%–30%.Simultaneously,BABA seed priming caused a noticeable decrease in the levels of hydrogen peroxide and lipid peroxidation products in the shoots of seedlings.The conclusion was made that the use of BABA as a bioregulator has the potential to enhance the germination of seeds with low sowing qualities.This is due to the ability of BABA to activate the metabolism of reserve substances in the grain and prevent the development of oxidative stress.
文摘The significance of gut microbiota(GM)in human health is being increasingly researched.An imbalance in GM composition,known as dysbiosis,is linked to various and other health issues.In addition,antibiotics are the primary and most significant factors leading to major changes in the composition and function of the GM,which may result in colonization by antimicrobial-resistant(AMR)pathogens.Therefore,alternative antibiotic strategies for combating AMR pathogens are urgently needed.This narrative review highlights current knowledge regarding various pertinent strategies for decolonizing bacterial pathogens from GM and emphasizes decolonization therapies’critical role in pediatric surgical disorders.Strategies such as decontamination of the digestive tract utilizing antibiotics,the use of probiotics,and particularly fecal microbiota transplantation have introduced new options for clinical treatment.These treatments show the potential to restore GM balance and have demonstrated advantages for intestinal disorders related to pediatric surgery,including inflammatory bowel disease,neonatal necrotizing enterocolitis,Hirschsprung-associated enterocolitis,and short bowel syndrome.Despite GM therapeutics,recent strategies are still in their developmental phase and exhibit challenges that need further research.Thus,potential future directions for GMtargeted decolonization therapies are under consideration.Innovative alternative strategies to combat AMR though GM modulation in disorders related to pediatric surgery appear to be promising and should continue to be prioritized for further research and development.
基金supported by the Brain Pool Program funded by the Ministry of Science and Information and Communication Technology through the National Research Foundation of Korea(Grant Nos.2022H1D3A2A01096185 and RS-2024-00410063)the Basic Science Research Program through the National Research Foundation of Korea(Grant No.RS-2023-00247376)+4 种基金the Cooperative Research Program for Agriculture Science and Technology Development,Korea(Grant No.RS-2022-RD010386)US National Science Foundation Plant Genome Program(Grant No.IOS-2048410)the US National Institute of General Medical Sciences of the National Institutes of Health(Grant No.R01GM121445)US Department of Agriculture’s National Institute of Food and Agriculture(Grant No.USDA-AFRI-2023-67013-39896)the National Science Foundation(Grant No.IOS-PGRP-2348319).
文摘Herbivorous insects and pathogens cause severe damage to rice tissues,affecting yield and grain quality.Damaged cells trigger downstream defense responses through various signals.Extracellular ATP(eATP),a signaling molecule released during mechanical cell damage,is considered a constitutive damage-associated molecular pattern(DAMP),which is crucial for initiating plant defense responses.Thus,understanding how rice plants cope with DAMPs such as eATP is essential.Here,we found that exogenous ATP affected rice growth and development,cell wall composition,chloroplast development,and cell death.Subsequent global transcriptome analysis revealed that several pathways were involved in the eATP response,including genes related to cell surface receptors,cell wall organization,chlorophyll biosynthesis,heat and temperature stimulation,epigenetic regulation,and reactive oxygen species metabolism.Cell surface receptors,including members of the lectin receptor-like kinases(LecRKs),were found to participate in the eATP response.We further investigated ATP-induced genes in T-DNA activation mutants of OsLecRKs,demonstrating their involvement in eATP signaling in rice.This study confirms a DAMP-mediated transcriptional response in plants and provides novel candidates for advancing resistant rice breeding against insect herbivores and pathogens.
基金supported by the National Research Council of Sri Lanka(Grant No.NRC TO 16-07).
文摘Decision Support Tool(DST)enables farmers to make site-specific crop management decisions;however,comprehensive calibration can be both costly and time-consuming.This study assessed the production and economic benefits of two calibrations of the Nutrient Expert(NE)tool for rice in Sri Lanka’s Alfisols:the basic calibration(Nutrient Expert Sri Lanka 1,NESL1)and the comprehensive calibration(Nutrient Expert Sri Lanka 2,NESL2).NESL1 was developed by adapting the South Indian version of NE to local conditions,while NESL2 was an updated version,using three years of data from 71 farmer fields.
基金supported by the Guizhou Provincial Department of Agriculture and Rural Afairs,the Guizhou Provincial Department of Science and Technology,and the Guizhou Provincial Department of Education.Funding Project are Guizhou Highland Specialty Vegetable Green Production Science,Technology Innovation Talent Team(Qiankehe Platform Talent-CXTD[2022]003)Guizhou Mountain Agriculture Key Core Technology Research Project(GZNYGJHX 2023013)Platform construction project of Engineering Research Center for Protected Vegetable Crops in Higher Learning Institutions of Guizhou Province(Qian Jiao Ji[2022]No.040).
文摘Houttuynia cordata, a characteristic edible and medicinal plant in southwestern China, is prone to absorbing lead (Pb^(2+)). Excessive consumption may lead to Pb^(2+) accumulation in the human body, which has been linked to serious health risks such as neurotoxicity, kidney damage, anemia, and developmental disorders, particularly in children. Therefore, the development of molecular markers associated with Pb^(2+) uptake and the investigation of the plant’s physiological responses to Pb^(2+) pollution are of great significance. In this study, 72 H. cordata germplasms were evaluated for Pb^(2+) accumulation after exogenous Pb^(2+) treatment. A significant variation in Pb^(2+) content was observed among the germplasms, indicating rich genetic diversity. Using RAPD markers, seven loci were identified to be significantly associated with Pb^(2+) uptake, with locus 43 (R^(2) = 6.72%) and locus 53 (R^(2) = 5.39%) showing the strongest correlations. Marker validation was performed using five low- and five high-accumulating accessions. Two representative germplasms were further subjected to 0, 500 and 1000 mg/kg Pb^(2+) treatments for 40 days. Pb^(2+) content, membrane lipid peroxidation, and redox enzyme activities (SOD, POD and CAT) were measured across different organs. Organs with greater soil contact (roots) exhibited higher Pb^(2+) accumulation and oxidative damage. POD and CAT activities were markedly induced by Pb^(2+) stress, while SOD response was limited. This study provides a theoretical foundation for breeding low Pb^(2+)-accumulating H. cordata varieties through marker-assisted selection (MAS) and supports their safe use and application in phytoremediation.
基金supported by the National Institute of Forest Sciencethe R&D Program for Forest Science Technology (No.2022458B10-2224-0201) of the Korea Forest Service
文摘Pinus densiflora is a pine species native to the Korean peninsula,and seed orchards have supplied mate-rial needed for afforestation in South Korea.Climate vari-ables affecting seed production have not been identified.The purpose of this study was to determine climate variables that influence annual seed production of two seed orchards using multiple linear regression(MLR),elastic net regres-sion(ENR)and partial least square regression(PLSR)mod-els.The PLSR model included 12 climatic variables from 2003 to 2020 and explained 74.3%of the total variation in seed production.It showed better predictive performance(R2=0.662)than the EN(0.516)and the MLR(0.366)mod-els.Among the 12 climatic variables,July temperature two years prior to seed production and July precipitation after one year had the strongest influence on seed production.The time periods indicated by the two variables corresponded to pollen cone initiation and female gametophyte development.The results will be helpful for developing seed collection plans,selecting new orchard sites with favorable climatic conditions,and investigating the relationships between seed production and climatic factors in related pine species.
基金A project funded in the framework of the Rural Development Plan 2014–2020 of the Emilia Romagna region,FOCUS AREA 3A-Misura 16.2.01–GREEN SUINO Project number 5415052.Coordinated from the Gruppo Martini SPA.
文摘Background The prolificacy of sows(litter size at birth)has markedly increased,leading to higher post-natal mor-tality.Heat stress can exacerbate this issue.Arginine plays an important role in several physiological pathways;its effect on gestating sows can depend on the period of supplementation.This study evaluated the effects of arginine supplementation on the productive performance and physiological status of sows during different gestation periods and seasons,using a multi-omics approach.Methods A total of 320 sows were divided into 4 groups over 2 seasons(warm/cold);a control group(CO)received a standard diet(including 16.5 g/d of arginine)and 3 other groups received the standard diet supplemented with 21.8 g/d of arginine(38.3 g/d of arginine)either during the first 35 d(Early35),the last 45 d(Late45)or through-out the entire gestation period(COM).The colostrum was analyzed for nutritional composition,immunoglobulins and metabolomic profile.Urine and feces were analyzed on d 35 and 106 for the metabolomic and microbial profiles.Piglet body weight and mortality were recorded at birth,d 6,d 26,and on d 14 post-weaning.Results Interactions between arginine and season were never significant.The Early35 group had a lower percent-age of stillborn(P<0.001),mummified(P=0.002)and low birthweight(LBW)piglets(P=0.02)than the CO group.The Late45 group had a lower percentage of stillborn piglets(P=0.029)and a higher percentage of high birthweight piglets(HBW;P<0.001)than the CO group.The COM group had a higher percentage of LBW(P=0.004)and crushed piglets(P<0.001)than the CO group.Arginine supplementation modifies the metabolome characterization of colos-trum,urine,and feces.Creatine and nitric oxide pathways,as well as metabolites related to microbial activity,were influenced in all matrices.A slight trend in the beta diversity index was observed in the microbiome profile on d 35(P=0.064).Conclusions Arginine supplementation during early gestation reduced the percentage of stillborn and LBW piglets,while in the last third of pregnancy,it favored the percentage of HBW pigs and reduced the percentage of stillbirths,showing that arginine plays a significant role in the physiology of pregnant sows.
文摘This study analyzes the causes and effects of climate change in the upper Niger River basin and the implementation of local adaptation strategies based on EMS(Environmental Management Systems).It aims to strengthen ecological resilience and sustainable natural resource management practices through training,awareness-raising,and community participation.The work was conducted in three rural communes in the Kissidougou prefecture,located in the Faranah administrative region.Data collection and analysis tools included questionnaires,GPS devices,digital devices,laptops,and Excel and SPSS software.The methodology employed a participatory and multidisciplinary approach combining a literature review,surveys of 163 respondents,semi-structured interviews with 16 key informants,training for 218 technical staff and local elected officials(30%of whom were women),and awareness-raising activities for 1,800 participants in local languages.Five community forests covering 443.58 hectares were integrated into management plans,concerted,under the coordination of the NGO APARFE.The results show an increase in average temperature(+0.8°C since 1960),a decrease in rainfall(-5.3 mm/month),and increased vulnerability of populations dependent on agriculture.The integration of the EMS(Environmental Management System)has led to improvements in environmental governance,community forest management,awareness of sustainable agricultural practices,and the inclusion of women(51%of participants).In short,the EMS is an effective tool for strengthening community resilience and environmental sustainability.
基金supported by Supported by National Key Laboratory of Cotton Bio-breeding and Integrated Utilization(CB2023C07)Xinjiang Autonomous Region"Three Agricultural"Backbone Talent Training Program(2022SNGGNT024)Xinjiang Huyanghe City Science and Technology Program(2023C08).
文摘Nitrogen(N)and phosphorus(P)are mineral nutrients essential for plant growth and development,playing a crucial role throughout the plant life cycle.Cotton,a globally significant textile crop,has a particularly high demand for N fertilizer across its developmental stages.This review explores the effects of adequate or deficient N and P levels on cotton growth phases,focusing on their influence on physiological processes and molecular mechanisms.Key topics include the regulation of N-and P-related enzymes,hormones,and genes,as well as the complex interplay of N-and P-related signaling pathways from the aspects of N-P signaling integration to regulate root development,N-P signaling integration to regulate nutrient uptake,and regulation of N-P interactions—a frontier in current research.Strategies for improving N and P use efficiency are also discussed,including developing high-efficiency cotton cultivars and identifying functional genes to enhance productivity.Generally speaking,we take model plants as a reference in the hope of coming up with new strategies for the efficient utilization of N and P in cotton.
文摘This study aimed to explore the relationship between Soil-Plant Analysis Development(SPAD)values and key environmental factors in cucumber(Cucumis sativus L.)cultivation in a greenhouse.SPAD values,indicative of chlorophyll content,reflect plant health and productivity.The analysis revealed strong positive correlations between SPADvalues and both indoor light intensity(ILI,r=0.59,p<0.001)and outdoor light intensity(OLI,r=0.62,p<0.001),suggesting that higher light intensities were associated with enhanced SPAD values.In contrast,significant negative correlations were found between SPAD values and soil temperature at 15-30 cm depth(ST1530,r=−0.47,p<0.001)and volumetric soil moisture content at the same depth(SM1530,r=−0.52,p<0.001),with higher soil temperatures(e.g.,28℃)and excessive moisture(e.g.,25%)leading to reduced SPAD values.Multiple regression analysis identified ST1530 and SM1530 as significant negative predictors of SPAD,with coefficients of−0.97(p=0.05)and−0.34(p=0.05),respectively,suggesting that increases in soil temperature and moisture result in lower SPAD values.Indoor light intensity(e.g.,600-800μmol/m^(2)/s)emerged as a significant positive contributor,with a coefficient of 0.01(p<0.001),highlighting its role in promoting chlorophyll synthesis.Additionally,relative humidity(r=0.27,p<0.01)showed a positive,although less pronounced,association with SPAD.These results underscore the importance of both direct and indirect environmental factors in influencing SPAD variability and,by extension,plant health and productivity in cucumber cultivation.
文摘In order to contribute to a better understanding of the biodiversity of local chicken populations, this study focused on the description of the essential qualitative parameters in the phenotypic characterization of local species. Conducted in 6 localities in the Far North Region of Cameroon (Doukoula, Yagoua, Guidiguis, Dziguilao, Maroua 3rd and Méri), a population of 240 local chickens, including 172 hens and 68 cocks were characterized in family farms. The choice of localities was made on the basis of their strong potential local chicken flocks in the region. To this end, each animal was the subject of a qualitative description based on the identification of the sex of the animal, the description of the colorations of the plumage and extremities, and the description of the types of format, plumage and crests. All observations were made with the naked eye and in daylight and then photographed. The main results show that the plumage colors are multiple and dominated by the White-Pied-Black (21.7%) and the Mille-fleur (20.8%);the wattles are dominated by the red (60%) and the pink (35.4%);the tarsi are dominated by the white (43.8%) and the black (32.08%);the white skin (92.5%) dominates over the pink skin (7.5%);the “Smooth-uniform” feather is dominant (97.08%), followed by the smooth-crested type (2.08%) and the fries type (0.82%);the medium size of the chickens is dominant (86.66%), followed by the dwarf size (9.58%) and the large size (3.75%). The results of this study demonstrate that there is a strong phenotypic diversity within the local chicken population. This diversity can serve as a basis for the development of selection, conservation and genetic potential improvement programs based on rational exploitation of the local chicken.
基金supported by Jiangsu Agriculture Science and Technology Innovation Fund(No.CX(22)3001)。
文摘The escalating global dissemination of plasmid-mediated antibiotic resistance poses a formidable threat to global health.Conjugation stands as a pivotal mechanism for horizontal gene transfer among bacterial populations,facilitating the spread of antibiotic resistance genes(ARGs).Microelectrolysis has garnered attention as an efficacious strategy for mitigating antibiotic concentrations in wastewater,yet its potential impact on ARG horizontal transfer remain largely unexplored.This comprehensive investigation unveils that microelectrolysis not only influences but significantly accelerates the conjugative transfer of ARG-harboring plasmids.Remarkably,this phenomenon is corroborated at the microbial community scale,underscoring its ecological relevance.Alarmingly,the study highlights the vulnerability of intestinalmicroorganisms to acquire antibiotic resistance under electrolytic stimulation,posing heightened risks to both animal and human health.Delving deeper,the study elucidates the underlyingmechanisms responsible for this enhanced conjugative transfer.It reveals that microelectrolysis augments the abundance of mating-competent cells,triggers the generation of reactive oxygen species,inflicts modest membrane damage,and upregulates the expression of genes critical for conjugation.These findings collectively contribute to a more profound comprehension of the environmental dissemination dynamics and associated public health implications of ARGs in the context of wastewater treatment employing microelectrolytic technologies.