Understory plants are an integral part of forests,serving a variety of functions that help maintain healthy ecosystems.The structure and composition of the understory are influenced by numerous biotic and abiotic fact...Understory plants are an integral part of forests,serving a variety of functions that help maintain healthy ecosystems.The structure and composition of the understory are influenced by numerous biotic and abiotic factors,with light being critical.The introduction of the pathogen Cronartium ribicola,which causes white pine blister rust,into North America in the early 20 th century led to the near total loss of western white pine(Pinus monticola)from moist forests of the Northern Rockies.Management is reintroducing blister rust-resistant western white pine across the landscape,but the effects on the understory are unknown.We examined the effects of stand structure and proportion of western white pine in the overstory on understory diversity of vascular plants in closed canopy stands dominated by blister rust-resistant western white pine across northern Idaho.Habitat series explained the greatest amount of variation(34%)in species presence-absence,while canopy cover accounted for 25%,basal area of all trees for 18%,and the proportion of western white pine composition by 14%.Our analysis revealed positive relationships between the proportion of western white pine in the overstory and both the presence of understory plants and the cover of several understory species.For both the presence and cover,separate sets of thirteen species were found to have a positive relationship with the proportion of western white pine in the overstory,with eight species in common.This research fills a knowledge gap by using data from a range of stands across northern Idaho with varying abundance of western white pine in the overstory to evaluate the relationship between the understory and overstory composition.As land managers plant more western white pine trees,we are likely to see the concomitant increase in understory plant diversity across the landscape,in addition to numerous other benefits,including disturbance resistance and resilience.展开更多
Triclosan(TCS) poses harmful risks to ecosystems and human health owing to its endocrine-disrupting effects.Therefore,developing an efficient and sustainable technology to degrade TCS is urgently needed.Herein,cobalt ...Triclosan(TCS) poses harmful risks to ecosystems and human health owing to its endocrine-disrupting effects.Therefore,developing an efficient and sustainable technology to degrade TCS is urgently needed.Herein,cobalt oxyhydroxide @covalent organic frameworks(CoOOH@COFs) S-scheme heterojunction was synthesized,which combined the visible-light-driven photocatalysis and peroxymonosulfate(PMS) activation to synergistically generate abundant reactive oxygen species(ROSs) for TCS degradation.The degradation efficiency of TCS reached 100 % within 8 min in the Vis-CoOOH@COFs/PMS system,and the reaction rate constant was 0.456 min^(-1),which was nearly 1.90 and 2.85 times that of single Co OOH and COFs,and2.36 times that under dark condition,respectively.The density functional theory(DFT) calculations confirmed the energy band bending of CoOOH@COFs and S-scheme charge transport from COFs to Co OOH.Both experimental and theoretical analyses indicated that Co OOH@COFs in photocatalytic-PMS activation systems synergistically facilitated photo-generated carrier separation,enhanced interfacial electron transfer,accelerated PMS activation,and generated multiple ROSs.In particular,photogenerated electrons(e^(-))accelerated the Co(Ⅲ)/Co(Ⅱ) redox cycle,while the PMS captured the e-,which significantly decreased the charge combination of Co OOH@COFs.Radicals(O_(2)^(·-),^(·)OH,and SO_(4)^(·-)) and non-radicals(such as ^(1)O_(2),h^(+),and e^(-)) were both presented in the Vis-CoOOH@COFs/PMS system,with O_(2)^(-) playing a dominant role in TCS degradation.Furthermore,the pathway of TCS degradation and toxicity of intermediates were explored by DFT calculation and transformation product identification.Importantly,the environmentally friendly CoOOH@COFs S-scheme heterojunction exhibited excellent stability and reusability.In conclusion,this study innovatively designed an S-scheme heterojunction in the photocatalytic-PMS activation system,providing guidance and theoretical support for efficient and eco-friendly wastewater treatment.展开更多
Improving the soil and biomass with coal is estimated at the international level as a way to enhance soil productiveness, fertility and also to mitigate climate change. Biochar employed to improve land scope and impou...Improving the soil and biomass with coal is estimated at the international level as a way to enhance soil productiveness, fertility and also to mitigate climate change. Biochar employed to improve land scope and impound carbon, is attracting a great deal of attention. Its characteristics of chemical, physical and biological properties, containing big surface area, CEC (Cation Exchange Capacity), high water-holding capacity, size of pore, volume, distribution, and element composition, affect its recognized influences, particularly on microbial communities. These are discovered in the agriculture lands, lands remediation and composting. However, incomplete information existed about biochar for several farmers or peasants in agriculture scope. Therefore, farmers or peasants and gardeners are facing new opportunities and defiance each day, from feeding global extending and expanding population, whilst meeting severe new emissions requirements, to create more food on fewer land area while reducing their environmental emissions. Widespread application and utilization of biochar in agricultural scope, forestry production, energy, environmental protection and additional areas, has interested awareness by scientists and investigators inside and/or outside the country. The objective of this paper is to provide a guide for the farmers or peasants and gardeners with an essential information about biochar and what the ability of biochar can be achieved in the soil, and which can provide the scientific reference for the biochar application, and to get high yield and good quality of crops in all of different soils.展开更多
Rain-fed agriculture depends on the groundwater as a supplementary source of irrigation. The poor quality of water from the hard rock area is applied to the crops to save the crop. Continuous irrigation leads to degra...Rain-fed agriculture depends on the groundwater as a supplementary source of irrigation. The poor quality of water from the hard rock area is applied to the crops to save the crop. Continuous irrigation leads to degradation of soil, drip irrigation system as well plants. This study assessed the damages on the drip irrigation system and soil, inflicted by the use of low-quality irrigation water. The quality of water was improved with reference to raw water in terms of pH (1.57% - 5.88%), EC (3.08% - 10.08%), ions (0.96% - 46%) by using magnetization method, without disrupting the existing irrigation system in the basaltic aquifer in semi-arid to the arid condition. This was demonstrated before the farmers in central India.展开更多
Global food demand requires that soils be used intensively for agriculture, but how these soils are managed greatly impacts soil fluxes of carbon dioxide (CO2). Soil management practices can cause carbon to be either ...Global food demand requires that soils be used intensively for agriculture, but how these soils are managed greatly impacts soil fluxes of carbon dioxide (CO2). Soil management practices can cause carbon to be either sequestered or emitted, with corresponding uncertain influence on atmospheric CO2 concentrations. The situation is further complicated by the lack of CO2 flux measurements for African subsistence farms. For widespread application in remote areas, a simple experimental methodology is desired. As a first step, the present study investigated the use of Bowen Ratio Energy Balance (BREB) instrumentation to measure the energy balance and CO2 fluxes of two contrasting crop management systems, till and no-till, in the lowlands within the mountains of Lesotho. Two BREB micrometeorological systems were established on 100-m by 100-m sites, both planted with maize (Zea mays) but under either conventional (plow, disk-disk) or no-till soil mangement systems. The results demonstrate that with careful maintenance of the instruments by appropriately trained local personnel, the BREB approach offers substantial benefits in measuring real time changes in agroecosystem CO2 flux. The periods where the two treatments could be compared indicated greater CO2 sequestration over the no-till treatments during both the growing and non-growing seasons.展开更多
Chemical insecticides have been considered as a means to combat crop pests. Although their effectiveness is evident, their impact on the environment is increasingly being discussed. The aim of this study is to determi...Chemical insecticides have been considered as a means to combat crop pests. Although their effectiveness is evident, their impact on the environment is increasingly being discussed. The aim of this study is to determine the agro-ecological potential of a biological insecticide (C<sub>25</sub>H<sub>32</sub>O<sub>12</sub>) based on Aloe barbadensis in a Sahelian context. For this purpose, a completely randomized block experimental design with 3 replications and 4 treatments was set up to experiment with Aloe barbadensis as a bioinsecticide against pests of Abelmoschus esculentus. However, data were collected using an observation and parameter monitoring grid. This includes the cultivation of Abelmoschus esculentus, soil preparation, seeding and watering, plot labeling, preparation of the bioinsecticide (selection and preparation of raw materials, grinding of Aloe barbadensis miller and extraction of the crude bioinsecticide, quantification of treatment doses and dilution, and obtaining the formulated bioinsecticide), plant watering, plant treatment, and finally parameter monitoring. The results obtained reveal that the level of damage is significantly high in the control treatment T0 (63%) compared to the other treatments, with 29% for treatment T1, 7% for T2, and 1% for T3, implying a strong action capability of this insecticide against pests of Abelmoschus esculentus. Therefore, it can be concluded that for a normal growing season of Abelmoschus esculentus, this biological insecticide should be sprayed 12 times. Furthermore, this biological insecticide is unique in that it does not inflict any gastric toxicity on the pests, which gives it the characteristic of being a repellent. It is a biological insecticide whose efficacy period has been tested, with a minimum duration of 21 days. In conclusion, this formulated bioinsecticide based on Aloe barbadensis demonstrates significant efficacy against pests of Abelmoschus esculentus. In the future, we will consider experimenting with its effectiveness against pests of other plants.展开更多
Emerging new races of wheat stem rust(Puccinia graminis f.sp.tritici)are threatening global wheat(Triticum aestivum L.)production.Host resistance is the most effective and environmentally friendly method of controllin...Emerging new races of wheat stem rust(Puccinia graminis f.sp.tritici)are threatening global wheat(Triticum aestivum L.)production.Host resistance is the most effective and environmentally friendly method of controlling stem rust.The stem rust resistance gene Sr59 was previously identified within a T2DS 2RL wheat-rye whole arm translocation,providing broad-spectrum resistance to various stem rust races.Seedling evaluation,molecular marker analysis,and cytogenetic studies identified wheat-rye introgression line#284 containing a new translocation chromosome T2BL 2BS-2RL.This line has demonstrated broad-spectrum resistance to stem rust at the seedling stage.Seedling evaluation and cytogenetic analysis of three backcross populations between the line#284 and the adapted cultivars SLU-Elite,Navruz,and Linkert confirmed that Sr59 is located within the short distal 2RL translocation.This study aimed physical mapping of Sr59 in the 2RL introgression segment and develop a robust molecular marker for marker-assisted selection.Using genotyping-by-sequencing(GBS),GBS-derived SNPs were aligned with full-length annotated rye nucleotide-binding leucine-rich repeat(NLR)genes in the parental lines CS ph1b,SLU238,SLU-Elite,Navruz,and Linkert,as well as in 33 BC4F5progeny.Four NLR genes were identified on the 2R chromosome,with Chr2R_NLR_60 being tightly linked to the Sr59resistance gene.In-silico functional enrichment analysis of the translocated 2RL region(25,681,915 bp)identified 223 genes,with seven candidate genes associated with plant disease resistance and three linked to agronomic performance,contributing to oxidative stress response,protein kinase activity,and cellular homeostasis.These findings facilitate a better understanding of the genetic basis of stem rust resistance provided by Sr59.展开更多
Edible oils derived from aquatic products are rich in lipids beneficial to human health.However,the volatile flavor characteristics of flesh oil and liver oil from Doederleinia berycoides remain unclear.In this study,...Edible oils derived from aquatic products are rich in lipids beneficial to human health.However,the volatile flavor characteristics of flesh oil and liver oil from Doederleinia berycoides remain unclear.In this study,flesh oil and liver oil were extracted from Doederleinia berycoides,revealing different fatty acid compositions and contents.Lipidomics analysis identified a total of 124 differential lipids between the flesh oil and liver oil,including 42 glycerophospholipids(GPs),33 glycerolipids(GLs),23 free fatty acids(FAs),13 sphingolipids(SPs),10 sterols(STs),and 3 prenol lipids(PRs).Analysis using HS-GC-IMS identified 12 key volatile compounds that significantly contributed to the distinct volatile flavors of the flesh and liver oils.The volatile flavors originated from these volatile compounds,which had different Relative Odor Activity Values(ROAVs).Further results from HSSPME-GC-MS showed that the volatile flavors of the flesh oil and liver oil were respectively attributed to 64 and 35 volatile compounds,each with unique key volatile compounds exhibiting different ROAVs.There were significant positive or negative correlations between 18 key differential lipids and 24 volatile compounds in both flesh oil and liver oil.Therefore,the complex lipid profiles are responsible for the unique volatile flavors of flesh oil and liver oil,and the differential lipids play a central role in their volatile flavor formation.These findings provide a foundation for understanding the volatile flavor differences in fish oils and hold promise for further exploration of the molecular mechanisms underlying oil volatile flavors.展开更多
The increasing global population has led to a higher demand for food production, while a decrease in rural labor and a rise in production costs present complex challenges for the food industry. Smart agriculture is a ...The increasing global population has led to a higher demand for food production, while a decrease in rural labor and a rise in production costs present complex challenges for the food industry. Smart agriculture is a farm management concept that considers the deployment of Internet of Things (IoT) to address current food production challenges. In this regard, the agricultural sector is becoming increasingly data-focused, and requires data and technologies that are more precise, advanced, and cutting-edge than in the past. IoT enables agriculture to become data-driven, resulting in timely and more cost-effective farm intervention while reducing environmental impact. This review provides an analytical survey of the current and potential applications of IoT in smart agriculture to overcome challenges posed by spatio-temporal variability under varying environments and task diversity. This review also discusses the challenges that may arise from IoT deployment and presents an overview of the existing applications and those that may be developed in the future.展开更多
Fresh-cut lettuce is widely used in ready-to-eat salads sold in modified atmosphere packages (MAP).Even in MAP,fresh-cut lettuce has short shelf life that results in loss of nutrients.Lettuce cultivars exhibit genetic...Fresh-cut lettuce is widely used in ready-to-eat salads sold in modified atmosphere packages (MAP).Even in MAP,fresh-cut lettuce has short shelf life that results in loss of nutrients.Lettuce cultivars exhibit genetic variation for shelf life in MAP,but their variation for nutrient retention is not known.Fifty accessions were evaluated for initial content of ascorbic acid (AsA),carotenoids,and sugars and their retention in storage.Accessions with high content and/or good retention of one or more nutrients were identified.The romaine accession ‘Floricos’ had high levels of all the three nutrients.Accessions with relatively high retention of all the three nutrients were ‘Salinas 88’,‘Siskiyou’,‘Solar’,SM09A,‘Romance’,and ‘Green Towers’.Romaine cultivars,‘Balady Barrage’,‘Green Towers’,and ‘Darkland’ had relatively high initial levels of all tested nutrients and good rate of their retention.There was no clear correlation between initial AsA/carotene concentrations and their retention rates,suggesting that besides content,retention of nutrients should also be a breeding target in a lettuce nutritional improvement program.Statistical analyses with the Pearson's correlation coefficient determined a negative relationship between tissue deterioration(AUDePS) and retention of all tested nutrients[r of-0.52 (P<0.0001) for AsA,-0.27 (P<0.01) for total carotene,and-0.59 (P<0.0001) for total sugars],suggesting that an increase in tissue deterioration intensifies nutrient decay.Broad-sense heritability (H^(2)) across the experiments was0.15 for AsA,0.23 for total carotene,and 0.50 for total sugars.Identification of germplasm with high nutrient content,extended shelf life and good nutrient retention provides valuable information for the lettuce industry and associated breeding programs.展开更多
β-Aminobutyric acid(BABA)is a physiologically active plant compound that has not been extensively studied.It has been shown to increase resistance to biotic and abiotic stress factors and enhance seed germination in ...β-Aminobutyric acid(BABA)is a physiologically active plant compound that has not been extensively studied.It has been shown to increase resistance to biotic and abiotic stress factors and enhance seed germination in certain plant species.However,its effects on cereal grains with low germination rates have not yet been studied.This study investigated the effects of BABA on the germination of aged triticale seeds,the metabolite content of seedlings,and the state of their antioxidant systems.The study found that a three-hour treatment of seeds in BABA solutions at concentrations ranging from 0.1 to 1.0 mM increased germination energy and germination(by 10%–14%)and enhanced the accumulation of shoot and root biomass(by 17%–26%).Additionally,amylase activity increased in the grains,and the accumulation of osmolytes(sugars and proline)increased in the shoots.The content of anthocyanins in shoots increased by almost twofold,and the activity of antioxidant enzymes(superoxide dismutase,catalase,and guaiacol peroxidase)increased by approximately 20%–30%.Simultaneously,BABA seed priming caused a noticeable decrease in the levels of hydrogen peroxide and lipid peroxidation products in the shoots of seedlings.The conclusion was made that the use of BABA as a bioregulator has the potential to enhance the germination of seeds with low sowing qualities.This is due to the ability of BABA to activate the metabolism of reserve substances in the grain and prevent the development of oxidative stress.展开更多
The significance of gut microbiota(GM)in human health is being increasingly researched.An imbalance in GM composition,known as dysbiosis,is linked to various and other health issues.In addition,antibiotics are the pri...The significance of gut microbiota(GM)in human health is being increasingly researched.An imbalance in GM composition,known as dysbiosis,is linked to various and other health issues.In addition,antibiotics are the primary and most significant factors leading to major changes in the composition and function of the GM,which may result in colonization by antimicrobial-resistant(AMR)pathogens.Therefore,alternative antibiotic strategies for combating AMR pathogens are urgently needed.This narrative review highlights current knowledge regarding various pertinent strategies for decolonizing bacterial pathogens from GM and emphasizes decolonization therapies’critical role in pediatric surgical disorders.Strategies such as decontamination of the digestive tract utilizing antibiotics,the use of probiotics,and particularly fecal microbiota transplantation have introduced new options for clinical treatment.These treatments show the potential to restore GM balance and have demonstrated advantages for intestinal disorders related to pediatric surgery,including inflammatory bowel disease,neonatal necrotizing enterocolitis,Hirschsprung-associated enterocolitis,and short bowel syndrome.Despite GM therapeutics,recent strategies are still in their developmental phase and exhibit challenges that need further research.Thus,potential future directions for GMtargeted decolonization therapies are under consideration.Innovative alternative strategies to combat AMR though GM modulation in disorders related to pediatric surgery appear to be promising and should continue to be prioritized for further research and development.展开更多
Objective IgA nephropathy(IgAN)is the most prevalent form of primary glomerular disease.However,its diagnosis is contingent on kidney biopsy.Therefore,noninvasive biomarkers are urgently needed for diagnosis.This stud...Objective IgA nephropathy(IgAN)is the most prevalent form of primary glomerular disease.However,its diagnosis is contingent on kidney biopsy.Therefore,noninvasive biomarkers are urgently needed for diagnosis.This study aims to identify novel urinary biomarkers that differentiate IgAN from other common primary glomerular diseases,specifically membranous nephropathy(MN)and minimal change disease(MCD).Methods The peripheral blood mononuclear cell(PBMC)transcriptome dataset GSE73953 was obtained from the GEO database.Differential gene expression,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment,Gene Ontology(GO)enrichment,and immune infiltration analyses were performed.Protein–protein interaction(PPI)analysis and lysosome-related genes were used to identify hub genes.The expression of the hub gene ATP6V1D in urine and kidney tissues from individuals with IgAN,healthy controls,MCD and MN patients was assessed using enzyme-linked immunosorbent assay(ELISA),Western blotting,and immunostaining techniques.Spearman’s correlation analysis was employed to investigate the relationships between the concentration of ATP6V1D in urine,the concentration of galactose-deficient IgA1(GD-IgA1),and the clinical data of patients.The receiver operating characteristic(ROC)curve was used to assess the role of urine ATP6V1D levels in distinguishing IgAN from MN and MCD.Results ATPase was identified as the principal intracellular structure associated with differentially expressed genes(DEGs)between IgAN patients and healthy controls in PBMCs.ATP6V1D was identified as a hub gene at the intersection of lysosome-related and differential genes.ATP6V1D levels were lower in PBMCs,urine,and kidney samples from IgAN patients than in those from healthy individuals,MCD and MN patients.The decreased urinary ATP6V1D levels and increased GD-IgA1 levels in IgAN patients were further validated.These changes were positively correlated with 24-h urine protein levels.Notably,a negative correlation was observed between ATP6V1D and GD-IgA1 levels.ROC curve analysis demonstrated that urinary ATP6V1D(AUC=0.972)and GD-IgA1(AUC=0.952)had significant discriminative power in distinguishing IgAN patients from MCD and MN patients,with no significant difference in predictive performance between the two biomarkers(P>0.05).Conclusions The findings underscore the potential utility of the urine ATP6V1D concentration as a biomarker to distinguish IgAN from MN and MCD.展开更多
Herbivorous insects and pathogens cause severe damage to rice tissues,affecting yield and grain quality.Damaged cells trigger downstream defense responses through various signals.Extracellular ATP(eATP),a signaling mo...Herbivorous insects and pathogens cause severe damage to rice tissues,affecting yield and grain quality.Damaged cells trigger downstream defense responses through various signals.Extracellular ATP(eATP),a signaling molecule released during mechanical cell damage,is considered a constitutive damage-associated molecular pattern(DAMP),which is crucial for initiating plant defense responses.Thus,understanding how rice plants cope with DAMPs such as eATP is essential.Here,we found that exogenous ATP affected rice growth and development,cell wall composition,chloroplast development,and cell death.Subsequent global transcriptome analysis revealed that several pathways were involved in the eATP response,including genes related to cell surface receptors,cell wall organization,chlorophyll biosynthesis,heat and temperature stimulation,epigenetic regulation,and reactive oxygen species metabolism.Cell surface receptors,including members of the lectin receptor-like kinases(LecRKs),were found to participate in the eATP response.We further investigated ATP-induced genes in T-DNA activation mutants of OsLecRKs,demonstrating their involvement in eATP signaling in rice.This study confirms a DAMP-mediated transcriptional response in plants and provides novel candidates for advancing resistant rice breeding against insect herbivores and pathogens.展开更多
Decision Support Tool(DST)enables farmers to make site-specific crop management decisions;however,comprehensive calibration can be both costly and time-consuming.This study assessed the production and economic benefit...Decision Support Tool(DST)enables farmers to make site-specific crop management decisions;however,comprehensive calibration can be both costly and time-consuming.This study assessed the production and economic benefits of two calibrations of the Nutrient Expert(NE)tool for rice in Sri Lanka’s Alfisols:the basic calibration(Nutrient Expert Sri Lanka 1,NESL1)and the comprehensive calibration(Nutrient Expert Sri Lanka 2,NESL2).NESL1 was developed by adapting the South Indian version of NE to local conditions,while NESL2 was an updated version,using three years of data from 71 farmer fields.展开更多
Houttuynia cordata, a characteristic edible and medicinal plant in southwestern China, is prone to absorbing lead (Pb^(2+)). Excessive consumption may lead to Pb^(2+) accumulation in the human body, which has been lin...Houttuynia cordata, a characteristic edible and medicinal plant in southwestern China, is prone to absorbing lead (Pb^(2+)). Excessive consumption may lead to Pb^(2+) accumulation in the human body, which has been linked to serious health risks such as neurotoxicity, kidney damage, anemia, and developmental disorders, particularly in children. Therefore, the development of molecular markers associated with Pb^(2+) uptake and the investigation of the plant’s physiological responses to Pb^(2+) pollution are of great significance. In this study, 72 H. cordata germplasms were evaluated for Pb^(2+) accumulation after exogenous Pb^(2+) treatment. A significant variation in Pb^(2+) content was observed among the germplasms, indicating rich genetic diversity. Using RAPD markers, seven loci were identified to be significantly associated with Pb^(2+) uptake, with locus 43 (R^(2) = 6.72%) and locus 53 (R^(2) = 5.39%) showing the strongest correlations. Marker validation was performed using five low- and five high-accumulating accessions. Two representative germplasms were further subjected to 0, 500 and 1000 mg/kg Pb^(2+) treatments for 40 days. Pb^(2+) content, membrane lipid peroxidation, and redox enzyme activities (SOD, POD and CAT) were measured across different organs. Organs with greater soil contact (roots) exhibited higher Pb^(2+) accumulation and oxidative damage. POD and CAT activities were markedly induced by Pb^(2+) stress, while SOD response was limited. This study provides a theoretical foundation for breeding low Pb^(2+)-accumulating H. cordata varieties through marker-assisted selection (MAS) and supports their safe use and application in phytoremediation.展开更多
Pinus densiflora is a pine species native to the Korean peninsula,and seed orchards have supplied mate-rial needed for afforestation in South Korea.Climate vari-ables affecting seed production have not been identified...Pinus densiflora is a pine species native to the Korean peninsula,and seed orchards have supplied mate-rial needed for afforestation in South Korea.Climate vari-ables affecting seed production have not been identified.The purpose of this study was to determine climate variables that influence annual seed production of two seed orchards using multiple linear regression(MLR),elastic net regres-sion(ENR)and partial least square regression(PLSR)mod-els.The PLSR model included 12 climatic variables from 2003 to 2020 and explained 74.3%of the total variation in seed production.It showed better predictive performance(R2=0.662)than the EN(0.516)and the MLR(0.366)mod-els.Among the 12 climatic variables,July temperature two years prior to seed production and July precipitation after one year had the strongest influence on seed production.The time periods indicated by the two variables corresponded to pollen cone initiation and female gametophyte development.The results will be helpful for developing seed collection plans,selecting new orchard sites with favorable climatic conditions,and investigating the relationships between seed production and climatic factors in related pine species.展开更多
Background The prolificacy of sows(litter size at birth)has markedly increased,leading to higher post-natal mor-tality.Heat stress can exacerbate this issue.Arginine plays an important role in several physiological pa...Background The prolificacy of sows(litter size at birth)has markedly increased,leading to higher post-natal mor-tality.Heat stress can exacerbate this issue.Arginine plays an important role in several physiological pathways;its effect on gestating sows can depend on the period of supplementation.This study evaluated the effects of arginine supplementation on the productive performance and physiological status of sows during different gestation periods and seasons,using a multi-omics approach.Methods A total of 320 sows were divided into 4 groups over 2 seasons(warm/cold);a control group(CO)received a standard diet(including 16.5 g/d of arginine)and 3 other groups received the standard diet supplemented with 21.8 g/d of arginine(38.3 g/d of arginine)either during the first 35 d(Early35),the last 45 d(Late45)or through-out the entire gestation period(COM).The colostrum was analyzed for nutritional composition,immunoglobulins and metabolomic profile.Urine and feces were analyzed on d 35 and 106 for the metabolomic and microbial profiles.Piglet body weight and mortality were recorded at birth,d 6,d 26,and on d 14 post-weaning.Results Interactions between arginine and season were never significant.The Early35 group had a lower percent-age of stillborn(P<0.001),mummified(P=0.002)and low birthweight(LBW)piglets(P=0.02)than the CO group.The Late45 group had a lower percentage of stillborn piglets(P=0.029)and a higher percentage of high birthweight piglets(HBW;P<0.001)than the CO group.The COM group had a higher percentage of LBW(P=0.004)and crushed piglets(P<0.001)than the CO group.Arginine supplementation modifies the metabolome characterization of colos-trum,urine,and feces.Creatine and nitric oxide pathways,as well as metabolites related to microbial activity,were influenced in all matrices.A slight trend in the beta diversity index was observed in the microbiome profile on d 35(P=0.064).Conclusions Arginine supplementation during early gestation reduced the percentage of stillborn and LBW piglets,while in the last third of pregnancy,it favored the percentage of HBW pigs and reduced the percentage of stillbirths,showing that arginine plays a significant role in the physiology of pregnant sows.展开更多
This study analyzes the causes and effects of climate change in the upper Niger River basin and the implementation of local adaptation strategies based on EMS(Environmental Management Systems).It aims to strengthen ec...This study analyzes the causes and effects of climate change in the upper Niger River basin and the implementation of local adaptation strategies based on EMS(Environmental Management Systems).It aims to strengthen ecological resilience and sustainable natural resource management practices through training,awareness-raising,and community participation.The work was conducted in three rural communes in the Kissidougou prefecture,located in the Faranah administrative region.Data collection and analysis tools included questionnaires,GPS devices,digital devices,laptops,and Excel and SPSS software.The methodology employed a participatory and multidisciplinary approach combining a literature review,surveys of 163 respondents,semi-structured interviews with 16 key informants,training for 218 technical staff and local elected officials(30%of whom were women),and awareness-raising activities for 1,800 participants in local languages.Five community forests covering 443.58 hectares were integrated into management plans,concerted,under the coordination of the NGO APARFE.The results show an increase in average temperature(+0.8°C since 1960),a decrease in rainfall(-5.3 mm/month),and increased vulnerability of populations dependent on agriculture.The integration of the EMS(Environmental Management System)has led to improvements in environmental governance,community forest management,awareness of sustainable agricultural practices,and the inclusion of women(51%of participants).In short,the EMS is an effective tool for strengthening community resilience and environmental sustainability.展开更多
Nitrogen(N)and phosphorus(P)are mineral nutrients essential for plant growth and development,playing a crucial role throughout the plant life cycle.Cotton,a globally significant textile crop,has a particularly high de...Nitrogen(N)and phosphorus(P)are mineral nutrients essential for plant growth and development,playing a crucial role throughout the plant life cycle.Cotton,a globally significant textile crop,has a particularly high demand for N fertilizer across its developmental stages.This review explores the effects of adequate or deficient N and P levels on cotton growth phases,focusing on their influence on physiological processes and molecular mechanisms.Key topics include the regulation of N-and P-related enzymes,hormones,and genes,as well as the complex interplay of N-and P-related signaling pathways from the aspects of N-P signaling integration to regulate root development,N-P signaling integration to regulate nutrient uptake,and regulation of N-P interactions—a frontier in current research.Strategies for improving N and P use efficiency are also discussed,including developing high-efficiency cotton cultivars and identifying functional genes to enhance productivity.Generally speaking,we take model plants as a reference in the hope of coming up with new strategies for the efficient utilization of N and P in cotton.展开更多
基金supported by the United States Department of Agriculture,Forest Service,Rocky Mountain Research Station through Research Joint Venture Agreement 17–098Funding was provided by the USDA Forest Service Northern Region。
文摘Understory plants are an integral part of forests,serving a variety of functions that help maintain healthy ecosystems.The structure and composition of the understory are influenced by numerous biotic and abiotic factors,with light being critical.The introduction of the pathogen Cronartium ribicola,which causes white pine blister rust,into North America in the early 20 th century led to the near total loss of western white pine(Pinus monticola)from moist forests of the Northern Rockies.Management is reintroducing blister rust-resistant western white pine across the landscape,but the effects on the understory are unknown.We examined the effects of stand structure and proportion of western white pine in the overstory on understory diversity of vascular plants in closed canopy stands dominated by blister rust-resistant western white pine across northern Idaho.Habitat series explained the greatest amount of variation(34%)in species presence-absence,while canopy cover accounted for 25%,basal area of all trees for 18%,and the proportion of western white pine composition by 14%.Our analysis revealed positive relationships between the proportion of western white pine in the overstory and both the presence of understory plants and the cover of several understory species.For both the presence and cover,separate sets of thirteen species were found to have a positive relationship with the proportion of western white pine in the overstory,with eight species in common.This research fills a knowledge gap by using data from a range of stands across northern Idaho with varying abundance of western white pine in the overstory to evaluate the relationship between the understory and overstory composition.As land managers plant more western white pine trees,we are likely to see the concomitant increase in understory plant diversity across the landscape,in addition to numerous other benefits,including disturbance resistance and resilience.
文摘Triclosan(TCS) poses harmful risks to ecosystems and human health owing to its endocrine-disrupting effects.Therefore,developing an efficient and sustainable technology to degrade TCS is urgently needed.Herein,cobalt oxyhydroxide @covalent organic frameworks(CoOOH@COFs) S-scheme heterojunction was synthesized,which combined the visible-light-driven photocatalysis and peroxymonosulfate(PMS) activation to synergistically generate abundant reactive oxygen species(ROSs) for TCS degradation.The degradation efficiency of TCS reached 100 % within 8 min in the Vis-CoOOH@COFs/PMS system,and the reaction rate constant was 0.456 min^(-1),which was nearly 1.90 and 2.85 times that of single Co OOH and COFs,and2.36 times that under dark condition,respectively.The density functional theory(DFT) calculations confirmed the energy band bending of CoOOH@COFs and S-scheme charge transport from COFs to Co OOH.Both experimental and theoretical analyses indicated that Co OOH@COFs in photocatalytic-PMS activation systems synergistically facilitated photo-generated carrier separation,enhanced interfacial electron transfer,accelerated PMS activation,and generated multiple ROSs.In particular,photogenerated electrons(e^(-))accelerated the Co(Ⅲ)/Co(Ⅱ) redox cycle,while the PMS captured the e-,which significantly decreased the charge combination of Co OOH@COFs.Radicals(O_(2)^(·-),^(·)OH,and SO_(4)^(·-)) and non-radicals(such as ^(1)O_(2),h^(+),and e^(-)) were both presented in the Vis-CoOOH@COFs/PMS system,with O_(2)^(-) playing a dominant role in TCS degradation.Furthermore,the pathway of TCS degradation and toxicity of intermediates were explored by DFT calculation and transformation product identification.Importantly,the environmentally friendly CoOOH@COFs S-scheme heterojunction exhibited excellent stability and reusability.In conclusion,this study innovatively designed an S-scheme heterojunction in the photocatalytic-PMS activation system,providing guidance and theoretical support for efficient and eco-friendly wastewater treatment.
文摘Improving the soil and biomass with coal is estimated at the international level as a way to enhance soil productiveness, fertility and also to mitigate climate change. Biochar employed to improve land scope and impound carbon, is attracting a great deal of attention. Its characteristics of chemical, physical and biological properties, containing big surface area, CEC (Cation Exchange Capacity), high water-holding capacity, size of pore, volume, distribution, and element composition, affect its recognized influences, particularly on microbial communities. These are discovered in the agriculture lands, lands remediation and composting. However, incomplete information existed about biochar for several farmers or peasants in agriculture scope. Therefore, farmers or peasants and gardeners are facing new opportunities and defiance each day, from feeding global extending and expanding population, whilst meeting severe new emissions requirements, to create more food on fewer land area while reducing their environmental emissions. Widespread application and utilization of biochar in agricultural scope, forestry production, energy, environmental protection and additional areas, has interested awareness by scientists and investigators inside and/or outside the country. The objective of this paper is to provide a guide for the farmers or peasants and gardeners with an essential information about biochar and what the ability of biochar can be achieved in the soil, and which can provide the scientific reference for the biochar application, and to get high yield and good quality of crops in all of different soils.
文摘Rain-fed agriculture depends on the groundwater as a supplementary source of irrigation. The poor quality of water from the hard rock area is applied to the crops to save the crop. Continuous irrigation leads to degradation of soil, drip irrigation system as well plants. This study assessed the damages on the drip irrigation system and soil, inflicted by the use of low-quality irrigation water. The quality of water was improved with reference to raw water in terms of pH (1.57% - 5.88%), EC (3.08% - 10.08%), ions (0.96% - 46%) by using magnetization method, without disrupting the existing irrigation system in the basaltic aquifer in semi-arid to the arid condition. This was demonstrated before the farmers in central India.
文摘Global food demand requires that soils be used intensively for agriculture, but how these soils are managed greatly impacts soil fluxes of carbon dioxide (CO2). Soil management practices can cause carbon to be either sequestered or emitted, with corresponding uncertain influence on atmospheric CO2 concentrations. The situation is further complicated by the lack of CO2 flux measurements for African subsistence farms. For widespread application in remote areas, a simple experimental methodology is desired. As a first step, the present study investigated the use of Bowen Ratio Energy Balance (BREB) instrumentation to measure the energy balance and CO2 fluxes of two contrasting crop management systems, till and no-till, in the lowlands within the mountains of Lesotho. Two BREB micrometeorological systems were established on 100-m by 100-m sites, both planted with maize (Zea mays) but under either conventional (plow, disk-disk) or no-till soil mangement systems. The results demonstrate that with careful maintenance of the instruments by appropriately trained local personnel, the BREB approach offers substantial benefits in measuring real time changes in agroecosystem CO2 flux. The periods where the two treatments could be compared indicated greater CO2 sequestration over the no-till treatments during both the growing and non-growing seasons.
文摘Chemical insecticides have been considered as a means to combat crop pests. Although their effectiveness is evident, their impact on the environment is increasingly being discussed. The aim of this study is to determine the agro-ecological potential of a biological insecticide (C<sub>25</sub>H<sub>32</sub>O<sub>12</sub>) based on Aloe barbadensis in a Sahelian context. For this purpose, a completely randomized block experimental design with 3 replications and 4 treatments was set up to experiment with Aloe barbadensis as a bioinsecticide against pests of Abelmoschus esculentus. However, data were collected using an observation and parameter monitoring grid. This includes the cultivation of Abelmoschus esculentus, soil preparation, seeding and watering, plot labeling, preparation of the bioinsecticide (selection and preparation of raw materials, grinding of Aloe barbadensis miller and extraction of the crude bioinsecticide, quantification of treatment doses and dilution, and obtaining the formulated bioinsecticide), plant watering, plant treatment, and finally parameter monitoring. The results obtained reveal that the level of damage is significantly high in the control treatment T0 (63%) compared to the other treatments, with 29% for treatment T1, 7% for T2, and 1% for T3, implying a strong action capability of this insecticide against pests of Abelmoschus esculentus. Therefore, it can be concluded that for a normal growing season of Abelmoschus esculentus, this biological insecticide should be sprayed 12 times. Furthermore, this biological insecticide is unique in that it does not inflict any gastric toxicity on the pests, which gives it the characteristic of being a repellent. It is a biological insecticide whose efficacy period has been tested, with a minimum duration of 21 days. In conclusion, this formulated bioinsecticide based on Aloe barbadensis demonstrates significant efficacy against pests of Abelmoschus esculentus. In the future, we will consider experimenting with its effectiveness against pests of other plants.
基金the financial support from FORMAS(2018-01029)the Swedish Institute(01132-2022)for supporting Ivan Motsnyi’s visit and research at Swedish University of Agricultural Sciences。
文摘Emerging new races of wheat stem rust(Puccinia graminis f.sp.tritici)are threatening global wheat(Triticum aestivum L.)production.Host resistance is the most effective and environmentally friendly method of controlling stem rust.The stem rust resistance gene Sr59 was previously identified within a T2DS 2RL wheat-rye whole arm translocation,providing broad-spectrum resistance to various stem rust races.Seedling evaluation,molecular marker analysis,and cytogenetic studies identified wheat-rye introgression line#284 containing a new translocation chromosome T2BL 2BS-2RL.This line has demonstrated broad-spectrum resistance to stem rust at the seedling stage.Seedling evaluation and cytogenetic analysis of three backcross populations between the line#284 and the adapted cultivars SLU-Elite,Navruz,and Linkert confirmed that Sr59 is located within the short distal 2RL translocation.This study aimed physical mapping of Sr59 in the 2RL introgression segment and develop a robust molecular marker for marker-assisted selection.Using genotyping-by-sequencing(GBS),GBS-derived SNPs were aligned with full-length annotated rye nucleotide-binding leucine-rich repeat(NLR)genes in the parental lines CS ph1b,SLU238,SLU-Elite,Navruz,and Linkert,as well as in 33 BC4F5progeny.Four NLR genes were identified on the 2R chromosome,with Chr2R_NLR_60 being tightly linked to the Sr59resistance gene.In-silico functional enrichment analysis of the translocated 2RL region(25,681,915 bp)identified 223 genes,with seven candidate genes associated with plant disease resistance and three linked to agronomic performance,contributing to oxidative stress response,protein kinase activity,and cellular homeostasis.These findings facilitate a better understanding of the genetic basis of stem rust resistance provided by Sr59.
基金supported by the R&D Projects in Key Areas of Guangdong Province(2023B0202080003)the National Natural Science Foundation of China(32472272,32302135,32072291)+1 种基金“Pioneer”and“Leading Goose”R&D Program of Zhejiang(2023C02006)Youth S&T Talent Support Programme of Guangdong Provincial Association for Science and Technology(SKXRC202401)。
文摘Edible oils derived from aquatic products are rich in lipids beneficial to human health.However,the volatile flavor characteristics of flesh oil and liver oil from Doederleinia berycoides remain unclear.In this study,flesh oil and liver oil were extracted from Doederleinia berycoides,revealing different fatty acid compositions and contents.Lipidomics analysis identified a total of 124 differential lipids between the flesh oil and liver oil,including 42 glycerophospholipids(GPs),33 glycerolipids(GLs),23 free fatty acids(FAs),13 sphingolipids(SPs),10 sterols(STs),and 3 prenol lipids(PRs).Analysis using HS-GC-IMS identified 12 key volatile compounds that significantly contributed to the distinct volatile flavors of the flesh and liver oils.The volatile flavors originated from these volatile compounds,which had different Relative Odor Activity Values(ROAVs).Further results from HSSPME-GC-MS showed that the volatile flavors of the flesh oil and liver oil were respectively attributed to 64 and 35 volatile compounds,each with unique key volatile compounds exhibiting different ROAVs.There were significant positive or negative correlations between 18 key differential lipids and 24 volatile compounds in both flesh oil and liver oil.Therefore,the complex lipid profiles are responsible for the unique volatile flavors of flesh oil and liver oil,and the differential lipids play a central role in their volatile flavor formation.These findings provide a foundation for understanding the volatile flavor differences in fish oils and hold promise for further exploration of the molecular mechanisms underlying oil volatile flavors.
文摘The increasing global population has led to a higher demand for food production, while a decrease in rural labor and a rise in production costs present complex challenges for the food industry. Smart agriculture is a farm management concept that considers the deployment of Internet of Things (IoT) to address current food production challenges. In this regard, the agricultural sector is becoming increasingly data-focused, and requires data and technologies that are more precise, advanced, and cutting-edge than in the past. IoT enables agriculture to become data-driven, resulting in timely and more cost-effective farm intervention while reducing environmental impact. This review provides an analytical survey of the current and potential applications of IoT in smart agriculture to overcome challenges posed by spatio-temporal variability under varying environments and task diversity. This review also discusses the challenges that may arise from IoT deployment and presents an overview of the existing applications and those that may be developed in the future.
基金supported by the Specialty Crop Block Grant Program of the U.S. Department of Agriculture's (USDA), Agricultural Marketing Service (AMS) through the California Department of Food and Agriculture (SCB15015)partly also by funding from the California Leafy Greens Research Board and by Oak Ridge Institute for Science and Education (ORISE) Research Participation Program。
文摘Fresh-cut lettuce is widely used in ready-to-eat salads sold in modified atmosphere packages (MAP).Even in MAP,fresh-cut lettuce has short shelf life that results in loss of nutrients.Lettuce cultivars exhibit genetic variation for shelf life in MAP,but their variation for nutrient retention is not known.Fifty accessions were evaluated for initial content of ascorbic acid (AsA),carotenoids,and sugars and their retention in storage.Accessions with high content and/or good retention of one or more nutrients were identified.The romaine accession ‘Floricos’ had high levels of all the three nutrients.Accessions with relatively high retention of all the three nutrients were ‘Salinas 88’,‘Siskiyou’,‘Solar’,SM09A,‘Romance’,and ‘Green Towers’.Romaine cultivars,‘Balady Barrage’,‘Green Towers’,and ‘Darkland’ had relatively high initial levels of all tested nutrients and good rate of their retention.There was no clear correlation between initial AsA/carotene concentrations and their retention rates,suggesting that besides content,retention of nutrients should also be a breeding target in a lettuce nutritional improvement program.Statistical analyses with the Pearson's correlation coefficient determined a negative relationship between tissue deterioration(AUDePS) and retention of all tested nutrients[r of-0.52 (P<0.0001) for AsA,-0.27 (P<0.01) for total carotene,and-0.59 (P<0.0001) for total sugars],suggesting that an increase in tissue deterioration intensifies nutrient decay.Broad-sense heritability (H^(2)) across the experiments was0.15 for AsA,0.23 for total carotene,and 0.50 for total sugars.Identification of germplasm with high nutrient content,extended shelf life and good nutrient retention provides valuable information for the lettuce industry and associated breeding programs.
基金support from the project of the Ministry of Education and Science of Ukraine 2-24-26 BO Development of Measures to Ensure Sustainable Productivity of Agrophytocenoses under the Influence of Abiotic and Biotic Stress Factors,state registration No.0124U000457.
文摘β-Aminobutyric acid(BABA)is a physiologically active plant compound that has not been extensively studied.It has been shown to increase resistance to biotic and abiotic stress factors and enhance seed germination in certain plant species.However,its effects on cereal grains with low germination rates have not yet been studied.This study investigated the effects of BABA on the germination of aged triticale seeds,the metabolite content of seedlings,and the state of their antioxidant systems.The study found that a three-hour treatment of seeds in BABA solutions at concentrations ranging from 0.1 to 1.0 mM increased germination energy and germination(by 10%–14%)and enhanced the accumulation of shoot and root biomass(by 17%–26%).Additionally,amylase activity increased in the grains,and the accumulation of osmolytes(sugars and proline)increased in the shoots.The content of anthocyanins in shoots increased by almost twofold,and the activity of antioxidant enzymes(superoxide dismutase,catalase,and guaiacol peroxidase)increased by approximately 20%–30%.Simultaneously,BABA seed priming caused a noticeable decrease in the levels of hydrogen peroxide and lipid peroxidation products in the shoots of seedlings.The conclusion was made that the use of BABA as a bioregulator has the potential to enhance the germination of seeds with low sowing qualities.This is due to the ability of BABA to activate the metabolism of reserve substances in the grain and prevent the development of oxidative stress.
文摘The significance of gut microbiota(GM)in human health is being increasingly researched.An imbalance in GM composition,known as dysbiosis,is linked to various and other health issues.In addition,antibiotics are the primary and most significant factors leading to major changes in the composition and function of the GM,which may result in colonization by antimicrobial-resistant(AMR)pathogens.Therefore,alternative antibiotic strategies for combating AMR pathogens are urgently needed.This narrative review highlights current knowledge regarding various pertinent strategies for decolonizing bacterial pathogens from GM and emphasizes decolonization therapies’critical role in pediatric surgical disorders.Strategies such as decontamination of the digestive tract utilizing antibiotics,the use of probiotics,and particularly fecal microbiota transplantation have introduced new options for clinical treatment.These treatments show the potential to restore GM balance and have demonstrated advantages for intestinal disorders related to pediatric surgery,including inflammatory bowel disease,neonatal necrotizing enterocolitis,Hirschsprung-associated enterocolitis,and short bowel syndrome.Despite GM therapeutics,recent strategies are still in their developmental phase and exhibit challenges that need further research.Thus,potential future directions for GMtargeted decolonization therapies are under consideration.Innovative alternative strategies to combat AMR though GM modulation in disorders related to pediatric surgery appear to be promising and should continue to be prioritized for further research and development.
基金supported by the Science and Technology Innovation Project of Hunan Province(No.2020SK51601)the Health Commission of Hunan Province Scientific Research Projects(No.202203054127)the Science and Technology Plan of Loudi City(No.202202).
文摘Objective IgA nephropathy(IgAN)is the most prevalent form of primary glomerular disease.However,its diagnosis is contingent on kidney biopsy.Therefore,noninvasive biomarkers are urgently needed for diagnosis.This study aims to identify novel urinary biomarkers that differentiate IgAN from other common primary glomerular diseases,specifically membranous nephropathy(MN)and minimal change disease(MCD).Methods The peripheral blood mononuclear cell(PBMC)transcriptome dataset GSE73953 was obtained from the GEO database.Differential gene expression,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment,Gene Ontology(GO)enrichment,and immune infiltration analyses were performed.Protein–protein interaction(PPI)analysis and lysosome-related genes were used to identify hub genes.The expression of the hub gene ATP6V1D in urine and kidney tissues from individuals with IgAN,healthy controls,MCD and MN patients was assessed using enzyme-linked immunosorbent assay(ELISA),Western blotting,and immunostaining techniques.Spearman’s correlation analysis was employed to investigate the relationships between the concentration of ATP6V1D in urine,the concentration of galactose-deficient IgA1(GD-IgA1),and the clinical data of patients.The receiver operating characteristic(ROC)curve was used to assess the role of urine ATP6V1D levels in distinguishing IgAN from MN and MCD.Results ATPase was identified as the principal intracellular structure associated with differentially expressed genes(DEGs)between IgAN patients and healthy controls in PBMCs.ATP6V1D was identified as a hub gene at the intersection of lysosome-related and differential genes.ATP6V1D levels were lower in PBMCs,urine,and kidney samples from IgAN patients than in those from healthy individuals,MCD and MN patients.The decreased urinary ATP6V1D levels and increased GD-IgA1 levels in IgAN patients were further validated.These changes were positively correlated with 24-h urine protein levels.Notably,a negative correlation was observed between ATP6V1D and GD-IgA1 levels.ROC curve analysis demonstrated that urinary ATP6V1D(AUC=0.972)and GD-IgA1(AUC=0.952)had significant discriminative power in distinguishing IgAN patients from MCD and MN patients,with no significant difference in predictive performance between the two biomarkers(P>0.05).Conclusions The findings underscore the potential utility of the urine ATP6V1D concentration as a biomarker to distinguish IgAN from MN and MCD.
基金supported by the Brain Pool Program funded by the Ministry of Science and Information and Communication Technology through the National Research Foundation of Korea(Grant Nos.2022H1D3A2A01096185 and RS-2024-00410063)the Basic Science Research Program through the National Research Foundation of Korea(Grant No.RS-2023-00247376)+4 种基金the Cooperative Research Program for Agriculture Science and Technology Development,Korea(Grant No.RS-2022-RD010386)US National Science Foundation Plant Genome Program(Grant No.IOS-2048410)the US National Institute of General Medical Sciences of the National Institutes of Health(Grant No.R01GM121445)US Department of Agriculture’s National Institute of Food and Agriculture(Grant No.USDA-AFRI-2023-67013-39896)the National Science Foundation(Grant No.IOS-PGRP-2348319).
文摘Herbivorous insects and pathogens cause severe damage to rice tissues,affecting yield and grain quality.Damaged cells trigger downstream defense responses through various signals.Extracellular ATP(eATP),a signaling molecule released during mechanical cell damage,is considered a constitutive damage-associated molecular pattern(DAMP),which is crucial for initiating plant defense responses.Thus,understanding how rice plants cope with DAMPs such as eATP is essential.Here,we found that exogenous ATP affected rice growth and development,cell wall composition,chloroplast development,and cell death.Subsequent global transcriptome analysis revealed that several pathways were involved in the eATP response,including genes related to cell surface receptors,cell wall organization,chlorophyll biosynthesis,heat and temperature stimulation,epigenetic regulation,and reactive oxygen species metabolism.Cell surface receptors,including members of the lectin receptor-like kinases(LecRKs),were found to participate in the eATP response.We further investigated ATP-induced genes in T-DNA activation mutants of OsLecRKs,demonstrating their involvement in eATP signaling in rice.This study confirms a DAMP-mediated transcriptional response in plants and provides novel candidates for advancing resistant rice breeding against insect herbivores and pathogens.
基金supported by the National Research Council of Sri Lanka(Grant No.NRC TO 16-07).
文摘Decision Support Tool(DST)enables farmers to make site-specific crop management decisions;however,comprehensive calibration can be both costly and time-consuming.This study assessed the production and economic benefits of two calibrations of the Nutrient Expert(NE)tool for rice in Sri Lanka’s Alfisols:the basic calibration(Nutrient Expert Sri Lanka 1,NESL1)and the comprehensive calibration(Nutrient Expert Sri Lanka 2,NESL2).NESL1 was developed by adapting the South Indian version of NE to local conditions,while NESL2 was an updated version,using three years of data from 71 farmer fields.
基金supported by the Guizhou Provincial Department of Agriculture and Rural Afairs,the Guizhou Provincial Department of Science and Technology,and the Guizhou Provincial Department of Education.Funding Project are Guizhou Highland Specialty Vegetable Green Production Science,Technology Innovation Talent Team(Qiankehe Platform Talent-CXTD[2022]003)Guizhou Mountain Agriculture Key Core Technology Research Project(GZNYGJHX 2023013)Platform construction project of Engineering Research Center for Protected Vegetable Crops in Higher Learning Institutions of Guizhou Province(Qian Jiao Ji[2022]No.040).
文摘Houttuynia cordata, a characteristic edible and medicinal plant in southwestern China, is prone to absorbing lead (Pb^(2+)). Excessive consumption may lead to Pb^(2+) accumulation in the human body, which has been linked to serious health risks such as neurotoxicity, kidney damage, anemia, and developmental disorders, particularly in children. Therefore, the development of molecular markers associated with Pb^(2+) uptake and the investigation of the plant’s physiological responses to Pb^(2+) pollution are of great significance. In this study, 72 H. cordata germplasms were evaluated for Pb^(2+) accumulation after exogenous Pb^(2+) treatment. A significant variation in Pb^(2+) content was observed among the germplasms, indicating rich genetic diversity. Using RAPD markers, seven loci were identified to be significantly associated with Pb^(2+) uptake, with locus 43 (R^(2) = 6.72%) and locus 53 (R^(2) = 5.39%) showing the strongest correlations. Marker validation was performed using five low- and five high-accumulating accessions. Two representative germplasms were further subjected to 0, 500 and 1000 mg/kg Pb^(2+) treatments for 40 days. Pb^(2+) content, membrane lipid peroxidation, and redox enzyme activities (SOD, POD and CAT) were measured across different organs. Organs with greater soil contact (roots) exhibited higher Pb^(2+) accumulation and oxidative damage. POD and CAT activities were markedly induced by Pb^(2+) stress, while SOD response was limited. This study provides a theoretical foundation for breeding low Pb^(2+)-accumulating H. cordata varieties through marker-assisted selection (MAS) and supports their safe use and application in phytoremediation.
基金supported by the National Institute of Forest Sciencethe R&D Program for Forest Science Technology (No.2022458B10-2224-0201) of the Korea Forest Service
文摘Pinus densiflora is a pine species native to the Korean peninsula,and seed orchards have supplied mate-rial needed for afforestation in South Korea.Climate vari-ables affecting seed production have not been identified.The purpose of this study was to determine climate variables that influence annual seed production of two seed orchards using multiple linear regression(MLR),elastic net regres-sion(ENR)and partial least square regression(PLSR)mod-els.The PLSR model included 12 climatic variables from 2003 to 2020 and explained 74.3%of the total variation in seed production.It showed better predictive performance(R2=0.662)than the EN(0.516)and the MLR(0.366)mod-els.Among the 12 climatic variables,July temperature two years prior to seed production and July precipitation after one year had the strongest influence on seed production.The time periods indicated by the two variables corresponded to pollen cone initiation and female gametophyte development.The results will be helpful for developing seed collection plans,selecting new orchard sites with favorable climatic conditions,and investigating the relationships between seed production and climatic factors in related pine species.
基金A project funded in the framework of the Rural Development Plan 2014–2020 of the Emilia Romagna region,FOCUS AREA 3A-Misura 16.2.01–GREEN SUINO Project number 5415052.Coordinated from the Gruppo Martini SPA.
文摘Background The prolificacy of sows(litter size at birth)has markedly increased,leading to higher post-natal mor-tality.Heat stress can exacerbate this issue.Arginine plays an important role in several physiological pathways;its effect on gestating sows can depend on the period of supplementation.This study evaluated the effects of arginine supplementation on the productive performance and physiological status of sows during different gestation periods and seasons,using a multi-omics approach.Methods A total of 320 sows were divided into 4 groups over 2 seasons(warm/cold);a control group(CO)received a standard diet(including 16.5 g/d of arginine)and 3 other groups received the standard diet supplemented with 21.8 g/d of arginine(38.3 g/d of arginine)either during the first 35 d(Early35),the last 45 d(Late45)or through-out the entire gestation period(COM).The colostrum was analyzed for nutritional composition,immunoglobulins and metabolomic profile.Urine and feces were analyzed on d 35 and 106 for the metabolomic and microbial profiles.Piglet body weight and mortality were recorded at birth,d 6,d 26,and on d 14 post-weaning.Results Interactions between arginine and season were never significant.The Early35 group had a lower percent-age of stillborn(P<0.001),mummified(P=0.002)and low birthweight(LBW)piglets(P=0.02)than the CO group.The Late45 group had a lower percentage of stillborn piglets(P=0.029)and a higher percentage of high birthweight piglets(HBW;P<0.001)than the CO group.The COM group had a higher percentage of LBW(P=0.004)and crushed piglets(P<0.001)than the CO group.Arginine supplementation modifies the metabolome characterization of colos-trum,urine,and feces.Creatine and nitric oxide pathways,as well as metabolites related to microbial activity,were influenced in all matrices.A slight trend in the beta diversity index was observed in the microbiome profile on d 35(P=0.064).Conclusions Arginine supplementation during early gestation reduced the percentage of stillborn and LBW piglets,while in the last third of pregnancy,it favored the percentage of HBW pigs and reduced the percentage of stillbirths,showing that arginine plays a significant role in the physiology of pregnant sows.
文摘This study analyzes the causes and effects of climate change in the upper Niger River basin and the implementation of local adaptation strategies based on EMS(Environmental Management Systems).It aims to strengthen ecological resilience and sustainable natural resource management practices through training,awareness-raising,and community participation.The work was conducted in three rural communes in the Kissidougou prefecture,located in the Faranah administrative region.Data collection and analysis tools included questionnaires,GPS devices,digital devices,laptops,and Excel and SPSS software.The methodology employed a participatory and multidisciplinary approach combining a literature review,surveys of 163 respondents,semi-structured interviews with 16 key informants,training for 218 technical staff and local elected officials(30%of whom were women),and awareness-raising activities for 1,800 participants in local languages.Five community forests covering 443.58 hectares were integrated into management plans,concerted,under the coordination of the NGO APARFE.The results show an increase in average temperature(+0.8°C since 1960),a decrease in rainfall(-5.3 mm/month),and increased vulnerability of populations dependent on agriculture.The integration of the EMS(Environmental Management System)has led to improvements in environmental governance,community forest management,awareness of sustainable agricultural practices,and the inclusion of women(51%of participants).In short,the EMS is an effective tool for strengthening community resilience and environmental sustainability.
基金supported by Supported by National Key Laboratory of Cotton Bio-breeding and Integrated Utilization(CB2023C07)Xinjiang Autonomous Region"Three Agricultural"Backbone Talent Training Program(2022SNGGNT024)Xinjiang Huyanghe City Science and Technology Program(2023C08).
文摘Nitrogen(N)and phosphorus(P)are mineral nutrients essential for plant growth and development,playing a crucial role throughout the plant life cycle.Cotton,a globally significant textile crop,has a particularly high demand for N fertilizer across its developmental stages.This review explores the effects of adequate or deficient N and P levels on cotton growth phases,focusing on their influence on physiological processes and molecular mechanisms.Key topics include the regulation of N-and P-related enzymes,hormones,and genes,as well as the complex interplay of N-and P-related signaling pathways from the aspects of N-P signaling integration to regulate root development,N-P signaling integration to regulate nutrient uptake,and regulation of N-P interactions—a frontier in current research.Strategies for improving N and P use efficiency are also discussed,including developing high-efficiency cotton cultivars and identifying functional genes to enhance productivity.Generally speaking,we take model plants as a reference in the hope of coming up with new strategies for the efficient utilization of N and P in cotton.
