Lactiplantibacillus plantarum MWFLp-182 was originally isolated from the feces of long-living elderly individuals in Hezhou city,Guangxi,China.L.plantarum MWFLp-182 showed favorable gastric and intestinal tolerance co...Lactiplantibacillus plantarum MWFLp-182 was originally isolated from the feces of long-living elderly individuals in Hezhou city,Guangxi,China.L.plantarum MWFLp-182 showed favorable gastric and intestinal tolerance compared with Lactiplantibacillus rhamnosus GG(LGG).L.plantarum MWFLp-182 was further examined for its antioxidant and anti-inflammatory activity in ABAP-treated HT-29 cells.Importantly,it enhanced the expression of anti-inflammatory factor(interleukin-10(IL-10)),antioxidant cytokines(superoxide dismutase(SOD),glutathione peroxidase(GPx),nuclear factor erythroid 2-related factor(Nrf2),and hemeoxygenase 1(HO-1)),B-cell lymphoma-2(Bcl-2),and tight junction(Zonula occludens protein 1(ZO-1),Claudin-1,and Occludin)proteins and genes,and reduced reactive oxygen species(ROS).The complete genome of L.plantarum MWFLp-182 contained a single circular chromosome that was 3257196 bp long,with a G+C content of 44.49%,and a single circular plasmid that was 53560 bp long.The genes tpx,trx A,trx B,npr,nrd H,dps,rec A,gpx,gsh A and ars C in this strain were related to antioxidant function;the key genes involved in antioxidant function were further investigated.In this study,we identified a probiotic candidate with antioxidant properties.展开更多
The aim of this paper was to study the effect of combination of Lactobacillus strains and tryptophan(Trp)-rich diet on the intestinal barrier function of Balb/c mice exposed to a cocktail of antibiotics and dextran so...The aim of this paper was to study the effect of combination of Lactobacillus strains and tryptophan(Trp)-rich diet on the intestinal barrier function of Balb/c mice exposed to a cocktail of antibiotics and dextran sodium sulfate.Several Lactobacillus strains isolated from the healthy human fecal sample was found to utilize Trp to produce indole derivatives.The results of Trp metabolism indicated that the ability of Lactobacillus to metabolize Trp to produce indole-3-lactic acid(ILA),indole-3-carboxaldehyde(I3C),and indole-3-acetic acid varies in vitro and in vivo.The effect of Lactobacillus with high-yielding indole derivatives on disease activity index,colon length,and intestinal permeability was significantly better than that of Lactobacillus with low-yielding indole derivatives in a high Trp diet.And Lactobacillus combined with Trp intervention also had a certain regulatory effect on the intestinal flora of male BALB/c mice.Among them,Lactiplantibacillus plantarum DPUL-S164 produced more ILA both in vivo and in vitro,and the combination of L.plantarum DPUL-S164 and Trp significantly decreased the expression level of the serum pro-inflammatory cytokine interleukin(IL)-6 and increased the expression level of the anti-inflammatory cytokine IL-10,significantly improved the number of goblet cells in the mouse mucous layer and increased mucin and tight junction protein expression.Furthermore,L.plantarum DPUL-S164 combined with Trp intervention activated the aryl hydrocarbon receptors(Ah R)signaling pathway.Furthermore,we found that the expression of colonic tight junction protein was positively correlated with the expression of colonic Ah R,and the expression of Ah R was positively correlated with the concentrations of ILA and I3C in vivo.Therefore,we conclude that the ILA as Ah R ligand produced by L.plantarum DPUL-S164 regulated the Ah R pathway,thus up-regulating the expression of the tight junction protein and protecting the integrity of the epithelial barrier.展开更多
Lactiplantibacillus plantarum Y42 exhibited good bioactivity in our previous study.In this study,the whole genome of L.plantarum Y42 was sequenced and its genome sequence information and function were analyzed to inve...Lactiplantibacillus plantarum Y42 exhibited good bioactivity in our previous study.In this study,the whole genome of L.plantarum Y42 was sequenced and its genome sequence information and function were analyzed to investigate its genotype and possible probiotic qualities.Thegenome of L.plantarum Y42 consisted of a circular 3.32 M bp chromosome,1 linear plasmid(0.013 M bp)and 1 ring-shaped plasmid(0.054 M bp),and contained a variety of genes associated with beneficial functions such as bacterial adhesion,acid tolerance,bile salt tolerance and heat tolerance.In particular,five genes associated with quorum sensing(QS)system(LuxS,Pfs,agrA,agrB,ftsH),three complete extracellular polysaccharide(EPS)synthesis gene clusters(cps2,cps3,cps4)and a cluster of pln genes encoding bacteriocins,which contains 21 genes,were identified in the L.plantarum Y42 genome.These results reveal the potential probiotic mechanism of L.plantarum Y42 at the gene level,and theoretically underlie its probiotic function excavation and practical application.展开更多
To investigate the effects of drinking the soaking of Malus domeri(Bois)Chev.leaves on gut microbiota and metabolites of long-living elderly individuals in Hezhou city,Guangxi,China.It has been reported that longevity...To investigate the effects of drinking the soaking of Malus domeri(Bois)Chev.leaves on gut microbiota and metabolites of long-living elderly individuals in Hezhou city,Guangxi,China.It has been reported that longevity is closely related to metabolism and the gut microbiota.The 16S rRNA sequencing and liquid chromatography-mass spectrometry(LC-MS)were used to analysis fecal samples and explore the factors affecting longevity in the region.Interestingly,we discovered,that elderly individuals who had been drinking the soaking of M.domeri(Bois)Chev.leaves for a long time exhibited higher diversity of the gut microbiota than without drinking the soaking,notably.The proportions of Ruminococcaceae and Prevotella were decreased in those who did not drink this soaking.In addition,a total of 106 metabolites were characterized,and the people of long-lived people(>90 years old)and elderly people(<90 years old)who drinking soaking of M.domeri(Bois)Chev.leaves significantly altered the gut microbiota and upregulated levels of haplopine,farnesol,genipic acid,momordicinin,2-hydroxyestrone,hydroxyphenyllactic acid,caffeic acid,sophoraflavanone B,and soyasaponin I.We preliminarily determined that M.domeri(Bois)Chev.leaves consumption may be an important factor affecting longevity in this area.展开更多
In this study,the microbial interactions among co-culture of Lactobacillus delbrueckii DPUL-F36,Lacticaseibacillus paracasei DPUL-F115,and Kluyveromyces marxianus 39 in fermented milk were evaluated during 13 days of ...In this study,the microbial interactions among co-culture of Lactobacillus delbrueckii DPUL-F36,Lacticaseibacillus paracasei DPUL-F115,and Kluyveromyces marxianus 39 in fermented milk were evaluated during 13 days of refrigerated storage(4±0.5◦C),in terms of titratable acidity,viable cell counts and metabolites.The fermented milk involving K.marxianus 39 showed a maximum lactobacilli count(4.8×10^(9) CFU/mL)and titratable acidity(151.0±1.7°T).K.marxianus 39 could enrich the flavor of the fermented milk and improve the sensory quality of the fermented milk on the 5th and 9th day of storage.The contents of aspartic acid,glycine,alanine,valine,methionine,leucine,lysine,and tyrosine increased significantly,while the contents of valine,methionine,leucine,phenylalanine,and lysine in milk fermented by Lactobacillus delbrueckii DPUL-F36,Lacticaseibacillus paracasei DPUL-F115,and Kluyveromyces marxianus 39 were significantly lower than those in milk fermented by Lactobacillus delbrueckii DPUL-F36,Lacticaseibacillus paracasei DPUL-F115 during storage.The higher concentrations of acetic acid,nonanoic acid,acetoin,2-nonanone,and 2-undecanol were observed in the fermented milk involved with K.marxianus 39.Our results showed K.marxianus 39 has the potential to be a starter culture for fermented dairy products.展开更多
基金supported by the Natural Science Foundation of Guangxi,China(2020GXNSFBA297083)the National Natural Science Foundation of China(32072193)。
文摘Lactiplantibacillus plantarum MWFLp-182 was originally isolated from the feces of long-living elderly individuals in Hezhou city,Guangxi,China.L.plantarum MWFLp-182 showed favorable gastric and intestinal tolerance compared with Lactiplantibacillus rhamnosus GG(LGG).L.plantarum MWFLp-182 was further examined for its antioxidant and anti-inflammatory activity in ABAP-treated HT-29 cells.Importantly,it enhanced the expression of anti-inflammatory factor(interleukin-10(IL-10)),antioxidant cytokines(superoxide dismutase(SOD),glutathione peroxidase(GPx),nuclear factor erythroid 2-related factor(Nrf2),and hemeoxygenase 1(HO-1)),B-cell lymphoma-2(Bcl-2),and tight junction(Zonula occludens protein 1(ZO-1),Claudin-1,and Occludin)proteins and genes,and reduced reactive oxygen species(ROS).The complete genome of L.plantarum MWFLp-182 contained a single circular chromosome that was 3257196 bp long,with a G+C content of 44.49%,and a single circular plasmid that was 53560 bp long.The genes tpx,trx A,trx B,npr,nrd H,dps,rec A,gpx,gsh A and ars C in this strain were related to antioxidant function;the key genes involved in antioxidant function were further investigated.In this study,we identified a probiotic candidate with antioxidant properties.
基金project was supported by the National Key Research and Development Program(2021YFD2100700)。
文摘The aim of this paper was to study the effect of combination of Lactobacillus strains and tryptophan(Trp)-rich diet on the intestinal barrier function of Balb/c mice exposed to a cocktail of antibiotics and dextran sodium sulfate.Several Lactobacillus strains isolated from the healthy human fecal sample was found to utilize Trp to produce indole derivatives.The results of Trp metabolism indicated that the ability of Lactobacillus to metabolize Trp to produce indole-3-lactic acid(ILA),indole-3-carboxaldehyde(I3C),and indole-3-acetic acid varies in vitro and in vivo.The effect of Lactobacillus with high-yielding indole derivatives on disease activity index,colon length,and intestinal permeability was significantly better than that of Lactobacillus with low-yielding indole derivatives in a high Trp diet.And Lactobacillus combined with Trp intervention also had a certain regulatory effect on the intestinal flora of male BALB/c mice.Among them,Lactiplantibacillus plantarum DPUL-S164 produced more ILA both in vivo and in vitro,and the combination of L.plantarum DPUL-S164 and Trp significantly decreased the expression level of the serum pro-inflammatory cytokine interleukin(IL)-6 and increased the expression level of the anti-inflammatory cytokine IL-10,significantly improved the number of goblet cells in the mouse mucous layer and increased mucin and tight junction protein expression.Furthermore,L.plantarum DPUL-S164 combined with Trp intervention activated the aryl hydrocarbon receptors(Ah R)signaling pathway.Furthermore,we found that the expression of colonic tight junction protein was positively correlated with the expression of colonic Ah R,and the expression of Ah R was positively correlated with the concentrations of ILA and I3C in vivo.Therefore,we conclude that the ILA as Ah R ligand produced by L.plantarum DPUL-S164 regulated the Ah R pathway,thus up-regulating the expression of the tight junction protein and protecting the integrity of the epithelial barrier.
基金supported by the National Natural Science Foundation of China(32072193).
文摘Lactiplantibacillus plantarum Y42 exhibited good bioactivity in our previous study.In this study,the whole genome of L.plantarum Y42 was sequenced and its genome sequence information and function were analyzed to investigate its genotype and possible probiotic qualities.Thegenome of L.plantarum Y42 consisted of a circular 3.32 M bp chromosome,1 linear plasmid(0.013 M bp)and 1 ring-shaped plasmid(0.054 M bp),and contained a variety of genes associated with beneficial functions such as bacterial adhesion,acid tolerance,bile salt tolerance and heat tolerance.In particular,five genes associated with quorum sensing(QS)system(LuxS,Pfs,agrA,agrB,ftsH),three complete extracellular polysaccharide(EPS)synthesis gene clusters(cps2,cps3,cps4)and a cluster of pln genes encoding bacteriocins,which contains 21 genes,were identified in the L.plantarum Y42 genome.These results reveal the potential probiotic mechanism of L.plantarum Y42 at the gene level,and theoretically underlie its probiotic function excavation and practical application.
基金supported by the National Natural Science Foundation of China (32072193)the Natural Science Foundation of Guangxi,China (2020GXNSFBA297083)。
文摘To investigate the effects of drinking the soaking of Malus domeri(Bois)Chev.leaves on gut microbiota and metabolites of long-living elderly individuals in Hezhou city,Guangxi,China.It has been reported that longevity is closely related to metabolism and the gut microbiota.The 16S rRNA sequencing and liquid chromatography-mass spectrometry(LC-MS)were used to analysis fecal samples and explore the factors affecting longevity in the region.Interestingly,we discovered,that elderly individuals who had been drinking the soaking of M.domeri(Bois)Chev.leaves for a long time exhibited higher diversity of the gut microbiota than without drinking the soaking,notably.The proportions of Ruminococcaceae and Prevotella were decreased in those who did not drink this soaking.In addition,a total of 106 metabolites were characterized,and the people of long-lived people(>90 years old)and elderly people(<90 years old)who drinking soaking of M.domeri(Bois)Chev.leaves significantly altered the gut microbiota and upregulated levels of haplopine,farnesol,genipic acid,momordicinin,2-hydroxyestrone,hydroxyphenyllactic acid,caffeic acid,sophoraflavanone B,and soyasaponin I.We preliminarily determined that M.domeri(Bois)Chev.leaves consumption may be an important factor affecting longevity in this area.
基金supported by the National Key Research and Development Plan(2022YFD2100700)the Major scientific and technological projects of Xinjiang Production and Construction Corps(no.2018AA009-02).
文摘In this study,the microbial interactions among co-culture of Lactobacillus delbrueckii DPUL-F36,Lacticaseibacillus paracasei DPUL-F115,and Kluyveromyces marxianus 39 in fermented milk were evaluated during 13 days of refrigerated storage(4±0.5◦C),in terms of titratable acidity,viable cell counts and metabolites.The fermented milk involving K.marxianus 39 showed a maximum lactobacilli count(4.8×10^(9) CFU/mL)and titratable acidity(151.0±1.7°T).K.marxianus 39 could enrich the flavor of the fermented milk and improve the sensory quality of the fermented milk on the 5th and 9th day of storage.The contents of aspartic acid,glycine,alanine,valine,methionine,leucine,lysine,and tyrosine increased significantly,while the contents of valine,methionine,leucine,phenylalanine,and lysine in milk fermented by Lactobacillus delbrueckii DPUL-F36,Lacticaseibacillus paracasei DPUL-F115,and Kluyveromyces marxianus 39 were significantly lower than those in milk fermented by Lactobacillus delbrueckii DPUL-F36,Lacticaseibacillus paracasei DPUL-F115 during storage.The higher concentrations of acetic acid,nonanoic acid,acetoin,2-nonanone,and 2-undecanol were observed in the fermented milk involved with K.marxianus 39.Our results showed K.marxianus 39 has the potential to be a starter culture for fermented dairy products.
