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PERMANENCE AND ASYMPTOTIC PROPERTIES OF NONLINEAR DELAY DIFFERENCE EQUATIONS
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作者 李万同 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 2003年第11期1273-1280,共8页
The asymptotic behavior of a class of nonlinear delay difference equation wax studied . Some sufficient conditions are obtained for permanence and global attractivity . The results can be applied to a claxs of nonline... The asymptotic behavior of a class of nonlinear delay difference equation wax studied . Some sufficient conditions are obtained for permanence and global attractivity . The results can be applied to a claxs of nonlinear delay difference equations and to the delay discrete Logistic model and some known results are included. 展开更多
关键词 global attractivity higher-order nonlinear difference equation PERMANENCE DELAY
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IN VIVO COMPARATIVE OBSERVATION ON THE INVASIVENESS OF VARIOUS ORGANS BY DIFFERENT LEUKEMIA CELLS
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作者 褚建新 应红光 丁立 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1990年第2期27-30,共4页
Using patho-morphological method and transplantation bio-assay, the in vivo invasiveness of leukemia cells is three transplantable mouse T cell leukemia models was comparatively studied. The results showed that the in... Using patho-morphological method and transplantation bio-assay, the in vivo invasiveness of leukemia cells is three transplantable mouse T cell leukemia models was comparatively studied. The results showed that the invasion to the liver was consistent, but that to other organs was obviously different. L615 and L7212 leukemia cells preferred to the bone marrow and spleen than to the peritoneum while L7811 leukemia cells were just the opposite. Transplantation bio-assay demonstrated that leukemia cells were present in the bone marrow of L615 mice as early as 6 hours after leukemic cell inoculation, but no leukemia cells was detected in bone marrow of L7811 mice 2 days after inoculation. In the terminal phase, L615 mice bone marrow became filled with leukemia cells, but L7811 mice bone marrow contained only a few leukemia cells. The difference of invasiveness of leukemia cells among organs is probably related to "homing" receptor. The same type of leukemia cells may possess multiple "homing" receptor. 展开更多
关键词 IN VIVO COMPARATIVE OBSERVATION ON THE INVASIVENESS OF VARIOUS ORGANS BY DIFFERENT LEUKEMIA CELLS bone
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DIFFERENCES IN EXPRESSION AND REGULATION BETWEEN TRANSFORMED CELLS OF THE HUMAN GASTRIC CARCINOMA ONCOGENE Ha-ras AND THE UNTRANSFORMED PARENT CELLS
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作者 韩复生 刘淑萍 +5 位作者 宋建国 袁艳华 张维 施华 邓国仁 刘培楠 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1989年第1期20-25,共6页
An Ha-ras oncogene was isolated from a cell line of gastric carcinoma called BGE-823 in order to elucidate genetic control and the influence of DNA sequences. The oncogene was cloned and identified as a single nucleot... An Ha-ras oncogene was isolated from a cell line of gastric carcinoma called BGE-823 in order to elucidate genetic control and the influence of DNA sequences. The oncogene was cloned and identified as a single nucleotide substitution of thymine for guanine in the 12th codon through the sequencing of its first axon. We compared the differences of expression and regulation between the transformed Ha-ras cells and untransformed parent cells. Data indicated that the expression of Ha-ras in the transformed cells was five-fold higher than in the untransformed cells and that the Ha-ras gene in the former was hypersensitive toward DNase I. In addition, a nuclear protein of 35 kilodaltons bound strongly to the 2.5 Kb fragment located upstream of the 6.6 Kb Ha-ras gene and contained a CC rich region. These results suggest that there might be another mechanism of activation for the ras gene besides point mutation. 展开更多
关键词 gene DIFFERENCES IN EXPRESSION AND REGULATION BETWEEN TRANSFORMED CELLS OF THE HUMAN GASTRIC CARCINOMA ONCOGENE Ha-ras AND THE UNTRANSFORMED PARENT CELLS
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Image Segmentation Based on Period Difference of the Oscillation
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作者 王直杰 张珏 +1 位作者 范宏 柯克峰 《Journal of Donghua University(English Edition)》 EI CAS 2004年第1期68-71,共4页
A new method for image segmentation based on pulse neural network is proposed. Every neuron in the network represents one pixel in the image and the network is locally connected. Each group of the neurons that corresp... A new method for image segmentation based on pulse neural network is proposed. Every neuron in the network represents one pixel in the image and the network is locally connected. Each group of the neurons that correspond to each object synchronizes while different groups of the neurons oscillate at different period. Applying this period difference, different objects are divided. In addition to simulation, an analysis of the mechanism of the method is presented in this paper. 展开更多
关键词 Image segmentation neural network SYNCHRONIZATION
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Determination of Teicoplanin A2's Partition Coefficient in Different Liquid-Liquid Extraction Systems 被引量:2
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作者 陈勇 Bin Mat Hanapi +2 位作者 徐志南 金志华 岑沛霖 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2004年第4期493-497,共5页
Teicoplanin is one of the macrocyclic glycopeptide antibiotics, which is active against Gram-positive bacteria, and has attracted a lot of attention in the field of chiral separation recently. In this work, the partit... Teicoplanin is one of the macrocyclic glycopeptide antibiotics, which is active against Gram-positive bacteria, and has attracted a lot of attention in the field of chiral separation recently. In this work, the partition coefficients and extraction ratio of teicoplanin in three different solvent systems were studied: conventional extraction, reactive extraction and reverse micelle extraction. With conventional solvent extraction, n-butanol demonstrated high partition coefficient for teicoplanin, but low extraction ratio because of its high solubility in water. Reactive extraction of teicoplanin showed the highest partition coefficient with almost 100% recovery in organic phase when tri-n-octylmethyl ammonium chloride (TOMAC) was used as extractant and pH value was above 5.0. A reverse micelle system, consisted of isooctane +10mmol-L-1 TOMAC +1% n-octanol, also offered high separation factor for teicoplanin. The results are beneficial for the design of teicoplanin separation and purification process. 展开更多
关键词 TEICOPLANIN partition coefficients conventional extraction reverse micelle extraction
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Measurement of the Average Molecular Weight of Cotton Cellulose Crosslinked by a Polycarboxylic Acid at Different pH Using Multiple Angle Light Scattering Photometer in a DMAc/LiCl Solvent System
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作者 毛志平 YANG Charles Q 《Journal of Donghua University(English Edition)》 EI CAS 2003年第4期5-7,共3页
Durable press finishing of cotton fabrics with polycarboxylic acid increases fabric wrinkle-resistance at the expense of its mechanical strength. Severe tensile strength loss is the major disadvantage for wrinkle resi... Durable press finishing of cotton fabrics with polycarboxylic acid increases fabric wrinkle-resistance at the expense of its mechanical strength. Severe tensile strength loss is the major disadvantage for wrinkle resistant cotton fabrics.Tensile strength loss of cotton fabric crosslinked by a polycarboxylic acid can be attributed to depolymerization and crosslink of cellulose molecules. Measurement of the molecular weight of cotton fabric before and after crosslinked by polycarboxylic acids can offer a possibility of direct understanding of the depolymerization. In this research, amultiple angle laser light scattering photometer was used todetermine the absolute molecular weight of cotton fabric treated with BTCA at different pH and then hydrolyzed with 0. 5 M NaOH solution at 50℃ for 144 h. The results indicate that average molecular weights of cotton fabric treated with polycarboxylic acids at different pH are almost the same. 展开更多
关键词 cotton cellulose DEPOLYMERIZATION laser light scattering photometer molecular weight
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Study on the Similarities and Differences of Body Measurement Terminology between ASTM and China GB Standard
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作者 方方 张渭源 张文斌 《Journal of Donghua University(English Edition)》 EI CAS 2003年第4期41-48,共8页
The similarities and differences of ASTM and China GB standard are studied in three aspects:measure instrument,terminology and applicable field.They are similar on the measuring apparatus and GB has less measurements,... The similarities and differences of ASTM and China GB standard are studied in three aspects:measure instrument,terminology and applicable field.They are similar on the measuring apparatus and GB has less measurements,such as girth,length and width measurements than ASTM and it lack across chest width,back width,total crotch length and shoulder slope which are important measurements in pattern making.ASTM classifies its standards according to the customers' size,gender and age.So we think GB standard could make some modifications from these fields to satisfy the users. 展开更多
关键词 Body measurement ANTHROPOMETRY Measuring standard PATTERN
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SPIO-enhanced magnetic resonance imaging for differentiating metastatic from hyperplastic lymph nodes: A study in rabbits
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作者 吴元魁 许乙凯 +3 位作者 黄其鎏 张嘉宁 吴凤林 刘杏元 《Journal of Medical Colleges of PLA(China)》 CAS 2003年第4期218-221,共4页
Objective: To investigate the potential of superparamagnetic iron oxide particles (SPIO) in MR imaging for the differentiation between hyperplastic and metastatic lymph node. Methods: Animal models of malignant lymph ... Objective: To investigate the potential of superparamagnetic iron oxide particles (SPIO) in MR imaging for the differentiation between hyperplastic and metastatic lymph node. Methods: Animal models of malignant lymph node metastasis were established in 6 New-Zealand rabbits by a unilateral intra-muscular injection of VX2 carcinoma cells, and models of hyperplastic lymph nodes were induced in another 6 rabbits by a unilateral intra-muscular injection of egg yolk emulsion. MR images of the lymph nodes were obtained before and 12 h after interstitial injection of SPIO. Image results were analyzed and compared with pathological findings. Results: On unenhanced images, the signal intensity of hyperplastic and metastatic lymph nodes did not differ significantly. After administration of SPIO, the signal intensity of both hyperplastic and metastatic lymph nodes remained unchanged on T1-weighted SE images. On T2-weighted SE images, the signal intensity of hyperplastic lymph nodes decreased heterogeneously, while that of all metastatic ones remained unchanged. On T2-weighted GRE images, the signal intensity of hyperplastic lymph nodes decreased significantly and homogeneously, while that of 4 metastatic ones remained unchanged and that of the rest 2 decreased heterogeneously. Conclusion: SPIO-enhanced MR imaging may enable the differentiation between the hyperplastic and metastatic lymph nodes. 展开更多
关键词 animal laboratory neoplasm metastasis contrast agent lymph node magnetic resonance imaging
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The Study of hemodynamics and coronary arteriography of Patients with Different Types of Unstable Angina Pectoris
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作者 谢小鲁 王燕妮 +1 位作者 祝家庆 袁祖贻 《South China Journal of Cardiology》 CAS 2003年第2期81-86,共6页
Objectives To explore the basic heart functional state and cardiac reserve function of patients with different types of unstable angina pectoris (UAP) and observe the relations between the heart function and severity ... Objectives To explore the basic heart functional state and cardiac reserve function of patients with different types of unstable angina pectoris (UAP) and observe the relations between the heart function and severity of coronary arterial disease. Methods 70 cases with UAP were enrolled including 25 patients with angina decubitus (AD), 23 patients with mixed angina (MA) , and 22 patients with accelerated effort angina (AEA). All patients underwent a series of examination such as UCG, ECT, hemodynamics and volume-loading test. The patients were divided into three groups in light of the results of the hemodynamic examination: ① diastolic dysfunction group ② systolic dysfunction group ③ normal heart function group. We assessed the basic heart function and cardiac reserve function of patients with different types of UAP and also observed the relations between coronary arteriography and heart function. Results ① Under basic conditions, patients with angina decubitus suffered from the systolic (36%) or diastolic dysfunction (48%). 43 percent of the patients with mixed angina had systolic dysfunction and other 43 percent of them had normal cardiac function. However, patients with accelerated effort angina (AEA) were characterized by diastolic dysfunction (31%) or normal heart function (60%). ② In hemodynamic examination, the pulmonary capillary wedge pressure of positive patients rose, at the same time cardiac index fell to the extreme instantaneously after loading in volume-loading test and then they restored to the basic level until 60 minutes. However, both PCWP and CI of the negative patients reach the peak momentarily after loading. They returned to the basic level within 30 minutes. ③ coronary arteriography show: there are 41% of patient with three-vessel disease, 50% with two-vessel disease, 9% single vessel disease and left main narrowing 22. 7% in AEA. There are separately 76%, 24%, 0% and 36% in AD; and 26.1% , 43.4% , 21.7% , 43.4% in MA. ④ Constituent ratio of the AEA and MA were separately compared with AD, AEA/AD: P < 0.05(P = 0.031); MA/AD: P < 0.01 (P = 0.000313). Conclusions Most of patients with the above three types of unstable angina pectoris suffered from the basic heart dysfunction and cardiac reserve dysfunction which might participate in the occurrence and development of unstable angina pectoris. In angiography, there are the most three-vessel diseases in AD that are, therefore, the most severe UAP. 展开更多
关键词 Unstable angina pectoris Hemodynamics Cardiac function Coronary arteriography
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RESPONSES OF THE YOUNG SPIKE OF WHEAT (TRITICUM AESTIVUM) AT DIFFERENT DEVELOPMENTAL STAGES TO CULTURE IN VITRO
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作者 舒理慧 《Chinese Science Bulletin》 SCIE EI CAS 1983年第3期397-400,共4页
Is there any difference between the abilities of differentiation and redifferentiation of tissues of wheat spike in the process of culture in vitro when it is at different developmental stages So far no reports about ... Is there any difference between the abilities of differentiation and redifferentiation of tissues of wheat spike in the process of culture in vitro when it is at different developmental stages So far no reports about this have been found in the literature. 展开更多
关键词 WHEAT DEVELOPMENTAL spike young roots EMPTY inoculated ORGANS FLORAL generative
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XPS STUDY ON DIFFERENT CRYSTALLINE FORMS OF COPPER PHTHALOCYANINE
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作者 王殿勋 石祖荣 《Chinese Science Bulletin》 SCIE EI CAS 1985年第5期612-615,共4页
The copper phthalocyanine (CuPc) as a dye and photoconductive material plays an important role in bionics. The different crystal forms of CuPc have distinct properties. In this article XPS results of α, β, γ, X-CuP... The copper phthalocyanine (CuPc) as a dye and photoconductive material plays an important role in bionics. The different crystal forms of CuPc have distinct properties. In this article XPS results of α, β, γ, X-CuPc have been reported. The character of shake-up satellite peaks and valence band spectra in these CuPc compounds was also examined. It was found that the difference of X-CuPc with other crystal forms of CuPc is obvious. XPS study on different crystal forms of CuPc has not been reported previously. 展开更多
关键词 SHAKE PHTHALOCYANINE VALENCE PHOTOELECTRON unsaturated listed CARLSON deter SUDDEN 加功
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DIFFERENCE SPECTRAL CHARACTERIZATION OF MICROSOMAL CYTOCHROME P-450 FROM LARVAE OF MOSQUITOES(Culex pipiens pallens Coq.)
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作者 沈建华 姜家良 《Chinese Science Bulletin》 SCIE EI CAS 1987年第6期408-412,共5页
Cytochrome P-450 is widely distributed in different species of animals. As a terminal oxidase ot the microsomal monooxygenase system, cytochrome P-450 is the key element of the enzyme system. Because of its broad subs... Cytochrome P-450 is widely distributed in different species of animals. As a terminal oxidase ot the microsomal monooxygenase system, cytochrome P-450 is the key element of the enzyme system. Because of its broad substrate specificity, it is involved in the biotransformation of many xenobiotic and endogenous compounds which are important in envitonmental protection, pharmacology and carcinogenicity. Microsomal P-450 is also a 展开更多
关键词 CULEX pharmacology endogenous CYTOCHROME specificity TROUGH tobacco LARVAE saturation insect
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DIFFERENTIATION OF ENTEROBACTERIA BY ISOTHERMAL PYROLYSIS GAS-LIQUID CHROMATOGRAPHY
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作者 金天如 谢少文 周方 《Chinese Science Bulletin》 SCIE EI CAS 1985年第3期417-420,共4页
A prominent feature for modern biological research is to make full use of different apparatus and techniques, such as spectrometry, chromatography, mass spectrometry and so on, which are hitherto chiefly used in the f... A prominent feature for modern biological research is to make full use of different apparatus and techniques, such as spectrometry, chromatography, mass spectrometry and so on, which are hitherto chiefly used in the fields of chemistry and physics. Gasliquid chromatography, as a new method for analytical chemistry, was invented by 展开更多
关键词 apparatus distilled hundred entero MINUTE PROTEUS indie eared stored NUTRIENT
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DIFFERENCE SCHEMES FOR FULLY NONLINEAR PSEUDO-HYPERBOLIC SYSTEMS 被引量:1
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作者 周毓麟 杜明笙 《Acta Mathematicae Applicatae Sinica》 SCIE CSCD 1991年第4期298-320,共23页
The general difference schemes for the first boundary problem of the fully nonlinear pseudo-hyperbolic systemsf(x, t, u,u_x,u_(xx),u_t,u_(tt),u_(xt),u_(xxt))=0are considered in the rectangular domain Q_T={0≤x≤1, 0≤... The general difference schemes for the first boundary problem of the fully nonlinear pseudo-hyperbolic systemsf(x, t, u,u_x,u_(xx),u_t,u_(tt),u_(xt),u_(xxt))=0are considered in the rectangular domain Q_T={0≤x≤1, 0≤t≤T}, where u(x,t)and f(x, t, u,p_1, p_2, r_1,r_2,q_1,q_2) are two m-dimensional vector functions with m≥1 for(x, t)∈Q_T and u,p_1,p_2,r_1,r_2,q_1,q_2∈R. The existence and the estimates of solutionsfor the finite difference system are established by the fixed point technique. The absolute andrelative stability and convergence of difference schemes are justified by means of a series of a prioriestimates. In the present study, the existence of unique smooth solution of the original problemis assumed. The similar results for nonlinear and quasilinear pseudo-hyperbolic systems are alsoobtained. 展开更多
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SAMPLING OF THE VISUAL INFORMATION BY THE NEURAL SUPERPOSITION COMPOUND EYES IN DIFFERENT ADAPTED STATES
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作者 吴卫国 郭爱克 +3 位作者 冯春华 石淑珍 王谷岩 吴盛立 《Chinese Science Bulletin》 SCIE EI CAS 1985年第5期668-671,共4页
Ⅰ. JNTRODUCTION Each ommatidium in the diptera compound eye contains 8 retinular cells. The first subsystem consists of 6 large retinular cells in periphery. Six different peripheral retinular cells from the 6 neighb... Ⅰ. JNTRODUCTION Each ommatidium in the diptera compound eye contains 8 retinular cells. The first subsystem consists of 6 large retinular cells in periphery. Six different peripheral retinular cells from the 6 neighbouring ommatidia receive visual information and transmit it into a single neuro-ommatidium (cartridge) of the first optic ganglion. The other two retinular cells are located at the centre of the ommatidium from the second subsystem, which receives visual information and sends it directy to the second optic ganglion. 展开更多
关键词 VISUAL transmit subsystem optic GANGLION PERIPHERY centre DISTAL PIGMENT elsewhere
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Laser-induced damage of 1064-nm narrow-band interference filters under different laser modes
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作者 高卫东 贺洪波 +1 位作者 邵建达 范正修 《Chinese Optics Letters》 SCIE EI CAS CSCD 2004年第8期493-494,共2页
The laser-induced damage behavior of narrow-band interference filters was investigated with a Nd:YAG laser at 1064 nm under single-pulse mode and free-running laser mode. The absorption measurement of such coatings ha... The laser-induced damage behavior of narrow-band interference filters was investigated with a Nd:YAG laser at 1064 nm under single-pulse mode and free-running laser mode. The absorption measurement of such coatings has been performed by surface thermal lensing (STL) technique. The relationship between damage morphology and absorption under the two different laser modes was studied in detail. The explanation was given by the standing-wave distribution theory. 展开更多
关键词 Laser modes Light interference Neodymium lasers Optical filters
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Simultaneously compensating tunable CD and adaptive PMD using two different nonlinearly chirped fiber Bragg gratings
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作者 于丽 张晓光 +4 位作者 周光涛 刘玉敏 张建忠 席丽霞 杨伯君 《Chinese Optics Letters》 SCIE EI CAS CSCD 2004年第8期445-448,共4页
Experimental research of simultaneous tunable chromatic dispersion (CD) and adaptive polarization mode dispersion (PMD) compensations in optical fiber communication system was reported. Two different nonlinearly chirp... Experimental research of simultaneous tunable chromatic dispersion (CD) and adaptive polarization mode dispersion (PMD) compensations in optical fiber communication system was reported. Two different nonlinearly chirped fiber Bragg gratings fabricated through the equivalent chirp technology were adopted in the experiment. One of the gratings was used as CD compensator, with a tunable dispersion range from 300 to 600 ps/nm. The other made of photosensitive polarization maintaining fiber was used as a tunable delay line of PMD compensator, which provided a varying amount of differential group delay (DGD) from 40 to 110 ps. Our experiment was operated at 10-Gb/s non-return-to-zero (NRZ) system and the results showed that the eye pattern recovery is excellent after both PMD and CD are compensated. Especially, the power penalty at a bit error rate (BER) of 10-9 is about 1 dB. 展开更多
关键词 Bit error rate Electromagnetic dispersion Fiber Bragg gratings
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Y染色体微缺失在先天性生殖系统疾病男性患儿中的分布及对睾丸发育的影响
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作者 梁妍 吕逸清 +3 位作者 黄轶晨 李嫔 许无恨 陈方 《浙江大学学报(医学版)》 北大核心 2025年第6期746-753,共8页
目的:分析先天性生殖系统疾病男性患儿Y染色体无精子症因子(AZF)微缺失的分布及对睾丸发育的影响。方法:前瞻性队列研究分析2021年11月至2023年12月上海交通大学医学院附属儿童医院泌尿外科收治的手术患儿,观察组为尿道下裂、隐睾、性... 目的:分析先天性生殖系统疾病男性患儿Y染色体无精子症因子(AZF)微缺失的分布及对睾丸发育的影响。方法:前瞻性队列研究分析2021年11月至2023年12月上海交通大学医学院附属儿童医院泌尿外科收治的手术患儿,观察组为尿道下裂、隐睾、性发育异常患儿,对照组为包茎、腹股沟斜疝或鞘膜积液患儿。收集患儿的血液标本,采用多重聚合酶链反应法对Y染色体AZF的15个位点进行检测。同时对观察组和对照组进行睾丸超声检查,记录睾丸位置及睾丸体积。经倾向评分匹配(PSM)和按照患儿年龄进行分层后,分析对照组与观察组间以及有无AZF微缺失患儿间睾丸体积的差异。结果:入组患儿共493例,其中观察组463例(尿道下裂372例,隐睾71例,性发育异常20例),对照组30例。对照组中未发现Y染色体微缺失病例,观察组中有4例患儿出现Y染色体微缺失,其中隐睾患儿1例(AZFc+AZFd区),孤立性尿道下裂患儿1例(AZFc区),性发育异常患儿2例(分别为AZFb+AZFc+AZFd区和AZFa区)。超声下测量睾丸888侧,PSM后对照组患儿的睾丸体积大于观察组(P<0.01)。分层分析结果显示,观察组有AZF微缺失患儿的睾丸体积有小于对照组和观察组无AZF微缺失患儿的趋势。结论:先天性生殖系统疾病男性患儿出现Y染色体AZF微缺失的概率大于普通人群,其中性发育异常患儿出现Y染色体AZF微缺失的概率大于隐睾及尿道下裂患儿。尿道下裂、隐睾和性发育异常及AZF微缺失均可能延缓患儿的睾丸发育。 展开更多
关键词 无精子症因子 Y染色体微缺失 隐睾 尿道下裂 性发育异常 睾丸体积
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表达绿色荧光蛋白的A型人源呼吸道合胞病毒反向遗传系统的构建
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作者 倪思睿 简书令 +3 位作者 余飞 易若男 武小琰 鲍恩东 《南京农业大学学报》 北大核心 2025年第6期1335-1342,共8页
[目的]为了更好地研究人呼吸道合胞病毒(HRSV)的发病机制并为其应用性研究提供便利,本研究构建了人源HRSV-A2毒株的反向遗传操作系统,并将绿色荧光蛋白(GFP)插入HRSV-A2基因组中,构建表达GFP的重组HRSV-A2。[方法]以细菌人工染色体(BAC... [目的]为了更好地研究人呼吸道合胞病毒(HRSV)的发病机制并为其应用性研究提供便利,本研究构建了人源HRSV-A2毒株的反向遗传操作系统,并将绿色荧光蛋白(GFP)插入HRSV-A2基因组中,构建表达GFP的重组HRSV-A2。[方法]以细菌人工染色体(BAC)为载体骨架构建HRSV-A2毒株全长质粒,并以此为基础在P与M基因之间插入GFP基因;以pCI为载体构建分别表达HRSV N、P、M2-1和L蛋白的4个辅助质粒,并将5个质粒共转染于表达T7 RNA聚合酶的BHK21-T7细胞系进行病毒拯救。[结果]提取rBAC-RSV-A2重组病毒和表达绿色荧光的rBAC-RSV-A2-P-GFP-M重组报告病毒RNA,通过RT-PCR进行全基因组测序,结果表明重组病毒构建成功;在F_(1)—F_(9)连续传代过程中均有绿色荧光表达,F_(10)代荧光消失且无细胞病变;测定重组病毒的生长曲线结果表明,2株重组病毒的生长趋势与原始毒的生长趋势大致相同,表明插入外源基因并不会改变病毒本身的生长特性;噬斑观察及染色结果显示,重组病毒的噬斑小于亲本病毒,表明重组病毒增殖水平下降。[结论]本研究成功构建了以BAC载体为骨架的HRSV-A2感染性克隆,获得了可以表达GFP的重组HRSV病毒。该研究所建立的高效、稳定的HRSV反向遗传学系统,为HRSV致病机制研究、疫苗和抗体药物研发提供了有益的技术平台。 展开更多
关键词 人呼吸道合胞病毒 绿色荧光蛋白 反向遗传系统 重组病毒
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基于水疱性口炎病毒载体的新型冠状病毒假病毒文库构建及免疫逃逸株筛选研究
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作者 曹高杰 彭飞 +4 位作者 简书令 许晨晖 王琳茜 柴柯柯 吴蓓蓓 《微生物与感染》 2025年第3期139-149,共11页
本研究构建了基于水疱性口炎病毒(vesicular stomatitis virus,VSV)载体的新型冠状病毒(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)S蛋白假病毒突变文库,以筛选潜在的免疫逃逸株,并解析其关键突变位点,旨在为疫苗研... 本研究构建了基于水疱性口炎病毒(vesicular stomatitis virus,VSV)载体的新型冠状病毒(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)S蛋白假病毒突变文库,以筛选潜在的免疫逃逸株,并解析其关键突变位点,旨在为疫苗研发和疫情防控提供科学依据。采用随机突变试剂盒对新型冠状病毒Wuhan-Hu-1株S蛋白的受体结合域(receptor binding domain,RBD)进行随机突变,构建假病毒质粒文库,并利用VSV载体实现病毒拯救。通过血清加压和有限稀释法筛选免疫逃逸株,并对其突变位点进行测序验证。结果显示,新型冠状病毒假病毒质粒文库成功构建,阳性率高达97.9%。本研究筛选出携带E484K、F486I、G339S、S477R等单突变的免疫逃逸株,以及E484K+K444Q、E484K+K462R等双突变的免疫逃逸株。部分逃逸株对中和血清表现出显著的抵抗能力,其中E484K单突变及E484K相关双突变株的中和效价显著下降。本研究证实通过构建新型冠状病毒假病毒突变文库成功筛选出具有免疫逃逸能力的变异株的可行性,并揭示其潜在的逃逸机制和流行特征。这些发现为理解新型冠状病毒的变异趋势提供了重要参考,并为疫苗研发和疫情防控策略的优化提供了科学支持。 展开更多
关键词 新型冠状病毒 免疫逃逸 随机突变文库 突变位点 疫苗研发
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