Objective: To evaluate the osteocompatibility of D, L-polylactic/hydroxyapatite/decalcifying bone matrix (PDLLA/HA/DBM), and compare with PDLLA and DBM. Methods: Human primary osteoblasts isolated from the femoral hea...Objective: To evaluate the osteocompatibility of D, L-polylactic/hydroxyapatite/decalcifying bone matrix (PDLLA/HA/DBM), and compare with PDLLA and DBM. Methods: Human primary osteoblasts isolated from the femoral head of patients were inoculated onto PDLLA/HA/DBM, PLA and DBM respectively. The proliferation rate and collagen Ⅰ expression were detected. The interface between biomaterial and osteoblasts was investigated with phase contrast microscopy and electron scanning microscopy. Results: Best proliferation rate was observed with the PDLLA/HA/DBM and followed by DBM and PLA, suggesting that PDLLA/HA/DBM satisfying most requirements for the cultivation of human osteoblasts. Scanning electron microscopy showed the morphology of osteoblasts was correlated with the proliferation data. The cells, well spread and flattened, were attached closely on the surface of biomaterial with an arched structure and had normal morphology. The extracellular collagenous matrixs covered the surface of biomaterial and packed the granules of biomaterial. Conclusion: PDLLA/HA/DBM can form osteointerface early and have a good biocompability.展开更多
In this paper,a radial basis function method combined with the stochastic Galerkin method is considered to approximate elliptic optimal control problem with random coefficients.This radial basis function method is a m...In this paper,a radial basis function method combined with the stochastic Galerkin method is considered to approximate elliptic optimal control problem with random coefficients.This radial basis function method is a meshfree approach for solving high dimensional random problem.Firstly,the optimality system of the model problem is derived and represented as a set of deterministic equations in high-dimensional parameter space by finite-dimensional noise assumption.Secondly,the approximation scheme is established by using finite element method for the physical space,and compactly supported radial basis functions for the parameter space.The radial basis functions lead to the sparsity of the stiff matrix with lower condition number.A residual type a posteriori error estimates with lower and upper bounds are derived for the state,co-state and control variables.An adaptive algorithm is developed to deal with the physical and parameter space,respectively.Numerical examples are presented to illustrate the theoretical results.展开更多
Methane-air diffusion filtration combustion in a radiative round-jet burner was numerically investigated in this work.The purpose of this study was focused on the effects of porous media porosity and gas velocity on t...Methane-air diffusion filtration combustion in a radiative round-jet burner was numerically investigated in this work.The purpose of this study was focused on the effects of porous media porosity and gas velocity on temperature distribution and CO and NO_(x)emissions.Reduced chemical kinetics was used where air and methane were assumed to be at their stoichiometric ratio,while thermo-physical properties were varied per the solid matrix porosity variation.Combustion characteristics were evaluated based on conduction and radiation as the two primary heat transfer modes within the solid matrix.Numerical simulations were carried out based on a packed bed with 3 mm alumina pellets.Results show that combustion temperature increases while the temperature gradient decreases with the increase in porosity,yielding higher NO_(x),and lower CO emissions.Furthermore,the combustion temperature is the lowest and most uniformly distributed with 1 m/s and 3 m/s gas velocities,wherewith 3 m/s gas velocity,combustion occurs outside of the porous zone.The corresponding NO_(x)and CO emissions are the lowest with 1 m/s gas velocity and increase with the increase in gas velocity from 1 m/s to 10m/s.展开更多
The centrosome is an organelle that serves as the microtubule-and actin-organizing center of human cells.Although the centrosome is small of size,it is great important on cellular function that regulates cytoskeletal ...The centrosome is an organelle that serves as the microtubule-and actin-organizing center of human cells.Although the centrosome is small of size,it is great important on cellular function that regulates cytoskeletal organization and governs precise spindle orientation/positioning ensuring equal distribution of cellular components in cell division.Epigenetic modifications to centrosome proteins can lead to centrosome aberrations,such as disorganized spindles and centrosome amplification causing aneuploidy and genomic instability.Epigenetic disturbances are associated not only with carcinogenesis and cancer progression,but also with drug resistance to chemotherapy.In this review,we discuss mechanisms of epigenetic alteration during the centrosome biogenesis in cancer.We provide an update on the current status of clinical trials that aim to target epigenetic modifications in centrosome aberrations and to thwart drug resistance.展开更多
Current antiepileptic drugs can inhibit seizure occurrence but are not effective in preventing its onset.Moreover,they produce several side effects,which may impact the efficacy of the treatment.In addition,it has bee...Current antiepileptic drugs can inhibit seizure occurrence but are not effective in preventing its onset.Moreover,they produce several side effects,which may impact the efficacy of the treatment.In addition,it has been stimulating the prospection of new molecules isolated from aromatic plants,with potential anticonvulsant and neuroprotective activities and less side effects.This study aimed to evaluate,though behavioural and neuro-chemical methodologies,the anticonvulsant,and neuroprotective effects of eucalyptol on mice subjected to the pilocarpine-induced seizure model.Eucalyptol(100,200 and 400 mg/kg,p.o.)was administered in mice prior to pilocarpine(350 mg/kg,i.p.)and the following behavioural parameters were assessed:Latency to First Seizure(LFS),Seizure Intensity(SI)and Latency to Death(LD).In addition,an oxotremorine-induced tremors test was performed to evaluate the cholinergic system involvement on the eucalyptol effects.Neurochemical tests were also performed,including determination of hippocampal(HC)concentration of thiobarbituric acid reactive substances(TBARS)and nitrite/nitrate and striatal(ST)concentration of noradrenaline,dopamine,and sero-tonin.Lastly,histopathological,and morphometric hippocampal analysis were conducted.Eucalyptol increased the latency to first seizure and latency to death,inhibited oxotremorine-induced tremors,decreased hippocampal TBARS and nitrite/nitrate overproduction,increased striatal noradrenaline and dopamine levels and prevented hippocampal neurodegeneration.These results demonstrate the potential anticonvulsant,neuroprotective and antioxidant effects of eucalyptol,probably through a conjunction of mechanisms including muscarinic cholin-ergic antagonism,oxidative stress mitigation and the monoaminergic system modulation,which appears to effectively control the seizure onset.展开更多
文摘Objective: To evaluate the osteocompatibility of D, L-polylactic/hydroxyapatite/decalcifying bone matrix (PDLLA/HA/DBM), and compare with PDLLA and DBM. Methods: Human primary osteoblasts isolated from the femoral head of patients were inoculated onto PDLLA/HA/DBM, PLA and DBM respectively. The proliferation rate and collagen Ⅰ expression were detected. The interface between biomaterial and osteoblasts was investigated with phase contrast microscopy and electron scanning microscopy. Results: Best proliferation rate was observed with the PDLLA/HA/DBM and followed by DBM and PLA, suggesting that PDLLA/HA/DBM satisfying most requirements for the cultivation of human osteoblasts. Scanning electron microscopy showed the morphology of osteoblasts was correlated with the proliferation data. The cells, well spread and flattened, were attached closely on the surface of biomaterial with an arched structure and had normal morphology. The extracellular collagenous matrixs covered the surface of biomaterial and packed the granules of biomaterial. Conclusion: PDLLA/HA/DBM can form osteointerface early and have a good biocompability.
基金supported by the National Natural Science Foundation of China(Grant Nos.11701253,11871312,12171042,11971259)by the Higher Education Key Platform and Research Project of Guangdong Province(The Key Project on the New Generation of Information Technology,No.2020zdzx3067)by the Natural Science Foundation of Shandong Province,China(Grant Nos.ZR2018MA007,ZR2023MA086,ZR2022MA005).
文摘In this paper,a radial basis function method combined with the stochastic Galerkin method is considered to approximate elliptic optimal control problem with random coefficients.This radial basis function method is a meshfree approach for solving high dimensional random problem.Firstly,the optimality system of the model problem is derived and represented as a set of deterministic equations in high-dimensional parameter space by finite-dimensional noise assumption.Secondly,the approximation scheme is established by using finite element method for the physical space,and compactly supported radial basis functions for the parameter space.The radial basis functions lead to the sparsity of the stiff matrix with lower condition number.A residual type a posteriori error estimates with lower and upper bounds are derived for the state,co-state and control variables.An adaptive algorithm is developed to deal with the physical and parameter space,respectively.Numerical examples are presented to illustrate the theoretical results.
文摘Methane-air diffusion filtration combustion in a radiative round-jet burner was numerically investigated in this work.The purpose of this study was focused on the effects of porous media porosity and gas velocity on temperature distribution and CO and NO_(x)emissions.Reduced chemical kinetics was used where air and methane were assumed to be at their stoichiometric ratio,while thermo-physical properties were varied per the solid matrix porosity variation.Combustion characteristics were evaluated based on conduction and radiation as the two primary heat transfer modes within the solid matrix.Numerical simulations were carried out based on a packed bed with 3 mm alumina pellets.Results show that combustion temperature increases while the temperature gradient decreases with the increase in porosity,yielding higher NO_(x),and lower CO emissions.Furthermore,the combustion temperature is the lowest and most uniformly distributed with 1 m/s and 3 m/s gas velocities,wherewith 3 m/s gas velocity,combustion occurs outside of the porous zone.The corresponding NO_(x)and CO emissions are the lowest with 1 m/s gas velocity and increase with the increase in gas velocity from 1 m/s to 10m/s.
基金This research was funded by university of Basel, Switzerland。
文摘The centrosome is an organelle that serves as the microtubule-and actin-organizing center of human cells.Although the centrosome is small of size,it is great important on cellular function that regulates cytoskeletal organization and governs precise spindle orientation/positioning ensuring equal distribution of cellular components in cell division.Epigenetic modifications to centrosome proteins can lead to centrosome aberrations,such as disorganized spindles and centrosome amplification causing aneuploidy and genomic instability.Epigenetic disturbances are associated not only with carcinogenesis and cancer progression,but also with drug resistance to chemotherapy.In this review,we discuss mechanisms of epigenetic alteration during the centrosome biogenesis in cancer.We provide an update on the current status of clinical trials that aim to target epigenetic modifications in centrosome aberrations and to thwart drug resistance.
基金supported by the Cearense Foundation for Support to Scientific(Fundaçāo Cearense de Apoio ao Desenvolvimento Científico e Tecnológico)[grant number:BMD-0008-01254.01.16/18,2018]the Regional University of Cariri.
文摘Current antiepileptic drugs can inhibit seizure occurrence but are not effective in preventing its onset.Moreover,they produce several side effects,which may impact the efficacy of the treatment.In addition,it has been stimulating the prospection of new molecules isolated from aromatic plants,with potential anticonvulsant and neuroprotective activities and less side effects.This study aimed to evaluate,though behavioural and neuro-chemical methodologies,the anticonvulsant,and neuroprotective effects of eucalyptol on mice subjected to the pilocarpine-induced seizure model.Eucalyptol(100,200 and 400 mg/kg,p.o.)was administered in mice prior to pilocarpine(350 mg/kg,i.p.)and the following behavioural parameters were assessed:Latency to First Seizure(LFS),Seizure Intensity(SI)and Latency to Death(LD).In addition,an oxotremorine-induced tremors test was performed to evaluate the cholinergic system involvement on the eucalyptol effects.Neurochemical tests were also performed,including determination of hippocampal(HC)concentration of thiobarbituric acid reactive substances(TBARS)and nitrite/nitrate and striatal(ST)concentration of noradrenaline,dopamine,and sero-tonin.Lastly,histopathological,and morphometric hippocampal analysis were conducted.Eucalyptol increased the latency to first seizure and latency to death,inhibited oxotremorine-induced tremors,decreased hippocampal TBARS and nitrite/nitrate overproduction,increased striatal noradrenaline and dopamine levels and prevented hippocampal neurodegeneration.These results demonstrate the potential anticonvulsant,neuroprotective and antioxidant effects of eucalyptol,probably through a conjunction of mechanisms including muscarinic cholin-ergic antagonism,oxidative stress mitigation and the monoaminergic system modulation,which appears to effectively control the seizure onset.
