Global food production faces enormous challenges in increasing yields while promoting environmental sustainability.A field experiments in the ecotone between the Yangtze River Basin and the HuangHuai-Hai Plain evaluat...Global food production faces enormous challenges in increasing yields while promoting environmental sustainability.A field experiments in the ecotone between the Yangtze River Basin and the HuangHuai-Hai Plain evaluated the effects of changing preceding crop rotation cycles(wheat and rapeseed)on long-term wheat-rice(W)and rapeseed-rice(R)rotation systems.A comprehensive evaluation of crop rotation systems was conducted using life cycle assessment,considering productivity,economic benefits,carbon footprint(CF),and soil health.Compared with fallow-rice rotation(F),alternating rapeseed and wheat rotations increased equivalent yield by 60.4%-82.2%,reduced CF by 0.3%-5.7%,and improved soil health by 0.3%-47.5%.Additionally,adding rapeseed to rotations increased soil nutrient content and raised soil organic carbon stocks by 31.3%-40.5%.The 3R rotation(3-year rapeseed-rice and 1-year wheat-rice)boosted rice yield by 82.2%and annual economic benefits by 84.4%,offering an effective model for optimizing long-term R rotations.Similarly,the 2W rotation(2-year wheat-rice and 1-year rapeseed rice)enhanced rice yield by 70.0% and annual economic benefits by 65.9%,providing a successful example for optimizing long-term W rotations.The 3R rapeseed-based rotation and the 2W wheatbased rotation demonstrated good environmental sustainability.These rotation systems have broad potential in sustainable intensive farming,especially in China and similar regions.展开更多
Brassica clubroot caused by Plasmodiophora brassicae has been identified as a severe soil-borne disease that poses a significant threat to plants root systems.The disease results in the formation of tumorous enlargeme...Brassica clubroot caused by Plasmodiophora brassicae has been identified as a severe soil-borne disease that poses a significant threat to plants root systems.The disease results in the formation of tumorous enlargements in the roots,leading to wilting and eventual plant death.Consequently,crop yield is drastically reduced,causing substantial economic losses in agriculture.The current study aims to provide a comprehensive overview of recent research process on Brassica clubroot,focusing on the biological characteristics,physiological race identification,and pathogenic mechanism of P.brassicae.Furthermore,it covers the latest advancements in the comprehensive prevention and clubroot control.The insights gained from this study are expected to contribute to the future research on clubroot and the development of resistance breeding strategies.展开更多
Eggplant(Solanum melongena L.)is a globally important vegetable crop,renowned for its nutritional value and economic significance.It is abundant in bioactive compounds such as anthocyanins and chlorogenic acid,which h...Eggplant(Solanum melongena L.)is a globally important vegetable crop,renowned for its nutritional value and economic significance.It is abundant in bioactive compounds such as anthocyanins and chlorogenic acid,which have been associated with multiple health-promoting properties(Azuma et al.,2008;Gurbuz et al.,2018).Given its significant hybrid vigor,F1 hybrid varieties are widely preferred in commercial cultivation(Mistry et al.,2018).However,traditional breeding practices predominantly rely on phenotypic selection,a process that is not only labor-intensive but also time-consuming.展开更多
The NRAMP(natural resistance-associated macrophage protein)family plays a pivotal role in metal ion transport,regulating both essential micronutrient uptake and toxic heavy metal accumulation in plants.In rice(Oryza s...The NRAMP(natural resistance-associated macrophage protein)family plays a pivotal role in metal ion transport,regulating both essential micronutrient uptake and toxic heavy metal accumulation in plants.In rice(Oryza sativa),OsNRAMP transporters critically influence metal homeostasis,stress adaptation,and grain safety.Among them,OsNRAMP5 serves as a major entry point for cadmium(Cd)and manganese(Mn)uptake,making it a prime target for low-Cd rice breeding.However,knockout of OsNRAMP5 leads to severe Mn deficiency,highlighting the need for precise genetic modifications(e.g.,OsNRAMP5-Q337K),which reduce Cd accumulation while maintaining Mn nutrition.Additionally,OsNRAMP1 and OsNRAMP2 contribute to Cd translocation and plant immunity,whereas OsNRAMP3/4/6/7 participate in Mn,iron,and zinc distribution and stress responses.This review systematically summarizes the structural,functional,and regulatory mechanisms of OsNRAMPs,emphasizing their roles in metal transport,pathogen resistance,and abiotic stress adaptation.Furthermore,we discuss strategies for developing low-Cd rice varieties,including QTL-based breeding,CRISPR/Cas9-mediated gene editing,and multi-gene stacking approaches.Finally,we outline future research directions,such as structural engineering of metal-binding sites and field validation of engineered rice lines,to ensure sustainable rice production in heavy metal-contaminated soils.展开更多
Clarifying the photosynthetic and population characteristics of conventional japonica rice at different yield levels is crucial for boosting yield and ensuring food security.Therefore,a two-year field trial with two c...Clarifying the photosynthetic and population characteristics of conventional japonica rice at different yield levels is crucial for boosting yield and ensuring food security.Therefore,a two-year field trial with two conventional japonica varieties was conducted at four planting densities:16 cm×30 cm(D1),14 cm×30 cm(D2),12 cm×30 cm(D3),and 10 cm×30 cm(D4).This study aimed to investigate how photosynthetic and population characteristics influence grain yield under varying planting densities.The results indicated that higher yields were primarily driven by increased grain weight and seed-setting rate(with a 9.68%‒11.40%higher single panicle weight),supported by optimized dry matter translocation and source-sink relationships.Elevated planting density(D2‒D4)enhanced panicle number and total spikelet number(by 3.91%‒15.00%)but reduced the number of spikelets per panicle,1000-grain weight,and photosynthetic efficiency due to mutual shading.Despite these trade-offs,yield increased by 4.10%‒12.42%under higher densities.The use of planting density D4 in japonica rice cultivation contributed to maximize yield.These findings provide important theoretical insights and practical significance for increasing the yield of conventional japonica rice and ensuring food security.展开更多
High temperature stress (HT) significantly reduces maize yield by impairing starch accumulation in kernels.However,the mechanism by which HT affects starch synthesis remains controversial-whether through reduced assim...High temperature stress (HT) significantly reduces maize yield by impairing starch accumulation in kernels.However,the mechanism by which HT affects starch synthesis remains controversial-whether through reduced assimilate supply or direct inhibition on kernel metabolism.To clarify these mechanisms,a heat-sensitive maize hybrid,Xianyu 335 (XY),was exposed to 30℃/20℃ (maximum/minimum temperature,control) and 40℃/30℃ for seven consecutive days during the seed setting stage.Synchronous pollination (SP),apical pollination (AP),and shading treatments were applied to manipulate the inherent source–sink ratio in maize plants.Results showed that apical kernel weight decreased by 11.9%under 40℃ in the SP treatment.The ^(13)C content,starch accumulation,and cell-wall invertase (CWIN) activity also declined by 15.9,36.7,and 16.4%,respectively,under HT.In the shading treatment,40℃/30℃ caused even greater reductions in^(13)C content,starch accumulation,and CWIN activity due to diminished assimilate supply.Conversely,in the AP treatment,starch content and CWIN activity increased by 22.0 and 18.5%,respectively,under 40℃/30℃,resulting in kernel weight and ^(13)C content similar to those in SP and shading treatments regardless of temperature.Consistent with apical kernels under AP,HT did not negatively affect middle kernels in either SP or shading treatments,as kernel weight and starch content remained unchanged under HT.Although all kernels were exposed to the same HT or control environment,their responses varied a lot.The impaired starch synthesis in apical kernels under HT was rescued by increasing carbon supply via AP treatment.The contrasting performance among middle kernels,apical kernels under AP,and apical kernels under SP or shading indicates that reduced carbon supply is a critical factor underlying inhibited starch accumulation.Our findings provide a theoretical basis for further understanding kernel abortion under HT.展开更多
Aspergillus species are ubiquitous fungi that produce mycotoxins(secondary metabolites)known as sterigmatocystin and aflatoxins in many different kinds of foods,which leads to serious contamination in agricultural pro...Aspergillus species are ubiquitous fungi that produce mycotoxins(secondary metabolites)known as sterigmatocystin and aflatoxins in many different kinds of foods,which leads to serious contamination in agricultural products,thereby endangering human health.Extensive studies on Aspergillus fungi have been conducted on growth and development,aflatoxin biosynthesis,and their interactions with environment.Here,we summarized a series of functional genes of the main Aspergillus fungi relative to toxins occurrence in foods,which revealed the signal transduction mechanisms of their involvement in growth and development,toxin production,and response to light,anticipating providing theoretical guidance on developing control and prevention technologies for mycotoxin contamination in agricultural products to ensure food safety.展开更多
Rapeseed(Brassica napus L.) is the largest oilseed crop in China and accounts for about 20% of world production.For the last 10 years,the production,planting area,and yield of rapeseed have been stable,with improvemen...Rapeseed(Brassica napus L.) is the largest oilseed crop in China and accounts for about 20% of world production.For the last 10 years,the production,planting area,and yield of rapeseed have been stable,with improvement of seed quality and especially seed oil content.China is among the leading countries in rapeseed genomic research internationally,having jointly with other countries accomplished the whole genome sequencing of rapeseed and its two parental species,Brassica oleracea and Brassica rapa.Progress on functional genomics including the identification of QTL governing important agronomic traits such as yield,seed oil content,fertility regulation,disease and insect resistance,abiotic stress,nutrition use efficiency,and pod shattering resistance has been achieved.As a consequence,molecular markers have been developed and used in breeding programs.During 2005–2014,215 rapeseed varieties were registered nationally,including 210 winter-and 5 spring-type varieties.Mechanization across the whole process of rapeseed production was investigated and operating instructions for all relevant techniques were published.Modern techniques for rapeseed field management such as high-density planting,controlled-release fertilizer,and biocontrol of disease and pests combined with precision tools such as drones have been developed and are being adopted in China.With the application of advanced breeding and production technologies,in the near future,the oil yield and quality of rapeseed varieties will be greatly increased,and more varieties with desirable traits,especially early maturation,high yield,high resistance to biotic and abiotic stress,and suitability for mechanized harvesting will be developed.Application of modern technologies on the mechanized management of rapeseed will greatly increase grower profit.展开更多
Sequence-specific nucleases(SSN) that generate double-stranded DNA breaks(DSBs) in genes of interest are the key to site-specific genome editing in plants. Genome editing has developed into one method of reducing unde...Sequence-specific nucleases(SSN) that generate double-stranded DNA breaks(DSBs) in genes of interest are the key to site-specific genome editing in plants. Genome editing has developed into one method of reducing undesirable traits in crops by the induction of knockout mutations. Different SSN-mediated genome-editing systems, including LAGLIDADG homing endonucleases or meganucleases, zinc-finger nucleases, transcription activator-like effector nucleases and clustered regularly interspaced short palindromic repeats, are emerging as robust tools for introducing functional mutations in polyploid crops including citrus, wheat, cotton, soybean, rapeseed, potato, grapes, Camelina sativa,dandelion, and tobacco. The approach utilizes knowledge of biological mechanisms for targeted induction of DSBs and their error-prone repair, allowing highly specific changes at designated genome loci. In this review, we briefly describe genome-editing technologies and their application to genetic improvement of polyploid crops.展开更多
Wheat streak mosaic virus（WSMV）has become a re-emerging pathogen in cereal crops in the Czech Republic.WSMV was first reported in the former Czechoslovakia in the early 1980s,and then no record of the virus was docu...Wheat streak mosaic virus（WSMV）has become a re-emerging pathogen in cereal crops in the Czech Republic.WSMV was first reported in the former Czechoslovakia in the early 1980s,and then no record of the virus was documented until 2009.The incidence of the virus was recorded in recent years in several winter wheat fields and many grass species.Here,we surveyed the incidence of WSMV in cereal crops.The results demonstrated the existence of the virus in winter wheat and volunteer wheat during each year of the monitoring period,which spanned from 2013–2016.Although the range of infected samples was low（6.4%of the total tested samples）,a high incidence of well-distributed virus was recorded.In at least six fields,the virus reached severe and potentially epidemic levels.In accordance with our previous report detailing WSMV infection of native grasses,we tested several grass species commonly grown in the Czech Republic.We found that some grass species acted as experimental hosts and possible reservoirs of the virus;these included Anthoxanthum odoratum（sweet vernal grass）,Arrhenatherum elatius（false oat-grass）,Lolium multiflorum（Italian rye-grass）,Bromus japonicus（Japanese chess）,Echinochloa crus-galli（barnyard grass）,Holcus lanatus（meadow soft grass）and Holcus mollis（creeping soft grass）.Some of these grass species are also important weeds of cereals,which may be the potential source of WSMV infection in cereal crops.Several widely used winter wheat cultivars were tested in the field after artificial inoculation with WSMV to evaluate virus titre by RT-qPCR.Overall,the tested cultivars had a low virus titre,which is associated with mild disease symptoms and may provide a good level of crop resistance to WSMV.展开更多
To address the global demand for rapeseed while considering farmers’profit,we face the challenges of making a quantum leap in seed yield and,at the same time,reducing yield loss due to biotic and abiotic stresses.We ...To address the global demand for rapeseed while considering farmers’profit,we face the challenges of making a quantum leap in seed yield and,at the same time,reducing yield loss due to biotic and abiotic stresses.We also face the challenge of efficiently applying new transformative biotechnology tools such as gene editing and breeding by genome design to increase rapeseed productivity and profitability.In this Perspective,we review advances in research on the physiological and genetic bases of both stress factorsaffected yield stability and seed yield potential,focusing on source–sink relationships and allocation of photosynthetic assimilates to vegetative growth and seed development.We propose research directions and highlight the role of plant architecture in the relative contributions of the root system,leaves,and pods to seed yield.We call for de novo design of new rapeseed crops.We review trait variation in existing germplasm and biotechnologies available for crop design.Finally,we discuss opportunities to apply fundamental knowledge and key germplasm to rapeseed production and propose an ideotype for de novo design of future rapeseed cultivars.展开更多
Peanut(Arachis hypogaea L.)is an important oil and cash crop in the world.Peanut germplasm collected in China are abundant,which provides important material guarantee for peanut breeding and industrial development.Her...Peanut(Arachis hypogaea L.)is an important oil and cash crop in the world.Peanut germplasm collected in China are abundant,which provides important material guarantee for peanut breeding and industrial development.Here,the safe conservation technology and indicators of peanut germplasm resources in the Oil Crops Middleterm Genebank of China were expounded from three processes of storage,monitoring,reproduction and renewal.We summarized and reviewed the situation of conservation and utilization of peanut germplasm resources in the Middle-term Genebank in the past 20 years.The future research direction of peanut resources in the Oil Crops Middle-term Genebank of China is prospected.展开更多
Chickpea(Cicer arietinum L.) and pigeonpea [Cajanus cajan L.(Millsp.)] play an important role in mitigating protein malnutrition for millions of poor vegetarians living in regions of the semi-arid tropics. Abiotic str...Chickpea(Cicer arietinum L.) and pigeonpea [Cajanus cajan L.(Millsp.)] play an important role in mitigating protein malnutrition for millions of poor vegetarians living in regions of the semi-arid tropics. Abiotic stresses such as excess and limited soil moisture(water-logging and drought), heat and chilling(high and low temperature stresses), soil salinity, and acidity are major yield constraints, as these two crops are grown mostly under rainfed conditions in risk-prone marginal and degraded lands with few or no inputs. Losses due to such stresses vary from 30% to 100% depending on their severity. The literature abounds in basic information concerning screening techniques, physiological mechanisms, and genetics of traits associated with resistance/tolerance to abiotic stresses in these two crops. However, the final outcome in terms of resistant/tolerant varieties has been far from satisfactory. This situation calls for improving selection efficiency through precise phenotyping and genotyping under high-throughput controlled conditions using modern tools of genomics. In this review, we suggest that an integrated approach combining advances from genetics, physiology, and biotechnology needs to be used for higher precision and efficiency of breeding programs aimed at improving abiotic stress tolerance in both chickpea and pigeonpea.展开更多
Drought is one of the most important environmental constraints limiting plant growth, development and crop yield. Many drought-inducible genes have been identified by molecular and genomic analyses in Arabidopsis, ric...Drought is one of the most important environmental constraints limiting plant growth, development and crop yield. Many drought-inducible genes have been identified by molecular and genomic analyses in Arabidopsis, rice and other crops. To better understand reaction mechanism of plant to drought tolerance, we mainly focused on introducing the research of transcription factors （TFs） in signal transduction and regulatory network of gene expression conferring drought. A TF could bind multiple target genes to increase one or more kinds of stress tolerance. Sometimes, several TFs might act together with a target gene. So drought-tolerance genes or TFs might respond to high-salinity, cold or other stresses. The crosstalk of multiple stresses signal pathways is a crucial aspect of understanding stress signaling.展开更多
Soybean(Glycine max)is one of the most important economic legume crops with largest planting area,and is also an important oil crop,as well as food and feed material.Soybean-rhizobia symbiosis plays important roles in...Soybean(Glycine max)is one of the most important economic legume crops with largest planting area,and is also an important oil crop,as well as food and feed material.Soybean-rhizobia symbiosis plays important roles in plant cultivation and fertilizer application.However,there are many problems in agricultural application of soybean symbiotic nitrogen fixation.In this review,we summarized three restriction factors(host specificity,low nitrogen fixation efficiency and abiotic stress)and discussed research progresses of these factors.Clarification of host specific mechanism will help to select and apply rhizobia inoculants.Both maintaining high nitrogenase activity and delaying nodule senescence can improve the efficiency of symbiotic nitrogen fixation.Abiotic stress-tolerant rhizobia can improve the abiotic stress tolerance of soybean.Breeding stress tolerant genotypes of soybean and rhizobia,obtaining correlated genes are the common strategies to improve soybean symbiotic nitrogen fixation under extreme conditions.Regulatory mechanisms of these restriction factors are still poorly understood and needs further clarification.展开更多
Among the crop production factors,preceding crop and tillage management affect the sustainable use of soil resources and ultimately crop growth and productivity.This study aimed at investigating the impact of precedin...Among the crop production factors,preceding crop and tillage management affect the sustainable use of soil resources and ultimately crop growth and productivity.This study aimed at investigating the impact of preceding winter crops(grass or legume)and different tillage systems on forage yield,quality and nutritive values of three summer grass(Sudan grass,pearl millet and teosinte)and two legume forage crops(cowpea and guar)under arid conditions.The results exhibited that growing forage crops after legumes(as berseem clover)produced the highest fresh and dry forage yields and quality attributes compared with grasses(as wheat)with the exception of crude fiber content,which was decreased.Moreover,tillage practices showed positive impact on forage yields and quality attributes.The maximum forage yields and quality parameters were recorded under conventional tillage(CT)practice compared with reduced tillage(RT)and no-tillage(NT)systems.Among the evaluated crops,the highest yields of fresh forage,dry forage,crude fiber,crude protein and total digestible nutrient were exhibited by grass forage crops(Sudan grass,pearl millet and teosinte),whereas the highest crude protein content and the digestible energy values were produced by legume forage crops(cowpea and guar).The maximum fresh forage,dry forage,crude fiber,crude protein,total digestible nutrient and digestible crude protein yields were produced by pearl millet followed by Sudan grass under CT and RT after berseem clover.The highest net return was recorded by sowing pearl millet after berseem clover and applying CT followed by RT practices,which could be recommended for the commercial production.Moreover,it could be assumed that the combination of growing grass forage crops after legume crops under CT or RT systems could enhance forage crop yield and quality with an improvement in soil properties for sustainable agriculture with low cost and the highest net income.展开更多
The basic question is whether our contemporary methods really describe exactly the “plant world” around us, experiments or if it is the result of the current “imperfect” level of pieces of knowledge. Frankly, expe...The basic question is whether our contemporary methods really describe exactly the “plant world” around us, experiments or if it is the result of the current “imperfect” level of pieces of knowledge. Frankly, experiments are often burdened with many errors in sampling, in the selection of experimental plants, at the simulation of environmental stress, in light composition and lighting methods, shortly by a lot of mistakes. Experimental conditions of individual authors in evaluating the same issue often differ—that is to say, results are difficult to compare. The artificial experimental environment should simulate selected average external conditions of a given crop. Very important is the identification of important growth phases and traits that are suitable for the evaluation of the main experimental task. Bad results and also conclusions of experiments can be by either ignorance of these facts or by incorrectly prepared experiments. Very important is also a knowledge level of concerning tested problematic. The main goal is not only to point out the need for measuring important properties of plants in a physiologically correct stage of growth and development, by suitable methods but also on the influence of the properties of utilized seeds in experiments that they can modify plant growth and development. It is often forgotten that impact of stress on the seeds’ properties and through the seed traits on the plant properties, for example, the same variety in of different provenance may give different results, especially influence on the roots, because plant roots are the most sensitive part of plants. Only the different origins of used seeds in identical experiments at two workplaces can significantly change the results of every repeated standard experiment.展开更多
As the core of the management of agricultural institutes,scientific research management plays a vital role in strategic development,and also affects the strategic development of agricultural research institutes. This ...As the core of the management of agricultural institutes,scientific research management plays a vital role in strategic development,and also affects the strategic development of agricultural research institutes. This paper expounds the connotation and characteristics of agricultural scientific research management as well as management ability requirements of management personnel. This paper puts forward the recommendations for improving agricultural scientific research management from the view of management personnel.展开更多
Peanut or groundnut （ Arachis hypogaea L. ） is an important source of vegetable oil in the world. Genetic enhancement for high yield and high oil content has greatly contributed to enhanced pro-ductivit...Peanut or groundnut （ Arachis hypogaea L. ） is an important source of vegetable oil in the world. Genetic enhancement for high yield and high oil content has greatly contributed to enhanced pro-ductivity of peanut and increased supply of peanut oil. Further improving oil content and quality of peanut is still crucial for increasing productivity of arable land and market competitiveness of peanut oil. Based on investigation among the peanut germplasm accessions including wild Arachis species, the oil content could be as high as 65%. Heterosis has been observed for oil content in hybrids derived from diverse crossing parents. Segregates with enhanced oil content have been obtained by pyramiding different genes or alleles with major and minor additive effects. Improved testing techniques for oil content in breeding lines with reduced cost have accelerated breeding progress for high oil content. SSR markers associated with oil content have been identified by association and linkage analysis. The stability of oil content in peanut across seasons and locations is highly associated with cold tolerance, high nutrition efficiency and drought tolerance. Recent progress on improving fatty acids in particular marker assisted backcrossing breeding has contributed to higher quality peanut oil and other products. High oil peanut lines with im-proved resistance to aflatoxin production have been developed. The wild Arachis species would be of great value for peanut breeding in increasing oil content.展开更多
The basic principle of Fenlong technology is to make full use of the cultivated layer and the below soil resources and natural precipitation. It can increase the amount of loosen soil by 1-2 times, water and oxygen by...The basic principle of Fenlong technology is to make full use of the cultivated layer and the below soil resources and natural precipitation. It can increase the amount of loosen soil by 1-2 times, water and oxygen by 1 time, and available nutrients by 10%-30%, surface air humidity by more than 10%, crop net photosynthetic efficiency by 10%-30%, biomass by 20%-30%, crop yield by 10%-50%, and crop quality by 5%, and decrease salt content by 20%-40% and the emission of methane and other gases by 10%. In this study, it is firstly proposed to establish a new research field——"Fenlong science of natural resources"(referred to as "Fenlong science"), and the research contents include Fenlong-based sciences of modern agricultural machinery and equipment for full-layer ploughing and bottom ploughing, tillage, crop cultivation, utilization of water resources (natural precipitation, farmland water conservancy, groundwater resources, etc.), conversion and utilization of saline-alkali land, arable degraded grassland reconstruction, ecological reconstruction of desertification land, ecological environment, climate change, economic and social development. It clarifies theory and enriches technology, and provides a major platform for carrying global population developing from more than 7 billion to 10 billion and helping people and nature to mutually benefit.展开更多
基金supported by the National Natural Science Foundation of China(31971855)the Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Science(CAAS-ASTIP-2021-OCRI)+2 种基金the Hubei Provincial Natural Science Foundation of China(2024AFB442)the Wuhan Knowledge Innovation Special Program(2023020201020400)the China Agriculture Research System(CARS-12)。
文摘Global food production faces enormous challenges in increasing yields while promoting environmental sustainability.A field experiments in the ecotone between the Yangtze River Basin and the HuangHuai-Hai Plain evaluated the effects of changing preceding crop rotation cycles(wheat and rapeseed)on long-term wheat-rice(W)and rapeseed-rice(R)rotation systems.A comprehensive evaluation of crop rotation systems was conducted using life cycle assessment,considering productivity,economic benefits,carbon footprint(CF),and soil health.Compared with fallow-rice rotation(F),alternating rapeseed and wheat rotations increased equivalent yield by 60.4%-82.2%,reduced CF by 0.3%-5.7%,and improved soil health by 0.3%-47.5%.Additionally,adding rapeseed to rotations increased soil nutrient content and raised soil organic carbon stocks by 31.3%-40.5%.The 3R rotation(3-year rapeseed-rice and 1-year wheat-rice)boosted rice yield by 82.2%and annual economic benefits by 84.4%,offering an effective model for optimizing long-term R rotations.Similarly,the 2W rotation(2-year wheat-rice and 1-year rapeseed rice)enhanced rice yield by 70.0% and annual economic benefits by 65.9%,providing a successful example for optimizing long-term W rotations.The 3R rapeseed-based rotation and the 2W wheatbased rotation demonstrated good environmental sustainability.These rotation systems have broad potential in sustainable intensive farming,especially in China and similar regions.
基金supported by the Science and Technology Talent Promotion Project(2023TJ-Z09)Innovation Program(2023RC1077,2023JJ40279)of Hunan Province,China.
文摘Brassica clubroot caused by Plasmodiophora brassicae has been identified as a severe soil-borne disease that poses a significant threat to plants root systems.The disease results in the formation of tumorous enlargements in the roots,leading to wilting and eventual plant death.Consequently,crop yield is drastically reduced,causing substantial economic losses in agriculture.The current study aims to provide a comprehensive overview of recent research process on Brassica clubroot,focusing on the biological characteristics,physiological race identification,and pathogenic mechanism of P.brassicae.Furthermore,it covers the latest advancements in the comprehensive prevention and clubroot control.The insights gained from this study are expected to contribute to the future research on clubroot and the development of resistance breeding strategies.
基金supported by Yuelushan Laboratory Breeding Program(Grant No.YLS-2025-ZY02013)The Project of National Key Laboratory for Tropical Crop Breeding(Grant No.NKLTCB202341)+4 种基金The New Variety Breeding Project of the Major Science and Technology Projects of Zhejiang(Grant No.2021C02065-1-3)Hunan Provincial Agricultural Science and Technology Innovation Fund Project(Grant No.2025CX115)Key R&D Projects in Hainan Province(Grant No.ZDYF2023XDNY041)Central Public-interest Scientific Institution Basal Research Fund(Grant No.1630062022003)2024 Sanya Technology Stars Program(Grant No.2024KJFX022).
文摘Eggplant(Solanum melongena L.)is a globally important vegetable crop,renowned for its nutritional value and economic significance.It is abundant in bioactive compounds such as anthocyanins and chlorogenic acid,which have been associated with multiple health-promoting properties(Azuma et al.,2008;Gurbuz et al.,2018).Given its significant hybrid vigor,F1 hybrid varieties are widely preferred in commercial cultivation(Mistry et al.,2018).However,traditional breeding practices predominantly rely on phenotypic selection,a process that is not only labor-intensive but also time-consuming.
基金supported by the National Key R&D Program,China(Grant No.2022YFD1201505)the Key Laboratory of Sichuan Province Open Project,China(Grant No.2023LYKF02)+1 种基金the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.CPSIBRF-CNRRI-202306)the Sichuan Provincial Financial Independent Innovation Project,China(Grant No.2022ZZCX001).
文摘The NRAMP(natural resistance-associated macrophage protein)family plays a pivotal role in metal ion transport,regulating both essential micronutrient uptake and toxic heavy metal accumulation in plants.In rice(Oryza sativa),OsNRAMP transporters critically influence metal homeostasis,stress adaptation,and grain safety.Among them,OsNRAMP5 serves as a major entry point for cadmium(Cd)and manganese(Mn)uptake,making it a prime target for low-Cd rice breeding.However,knockout of OsNRAMP5 leads to severe Mn deficiency,highlighting the need for precise genetic modifications(e.g.,OsNRAMP5-Q337K),which reduce Cd accumulation while maintaining Mn nutrition.Additionally,OsNRAMP1 and OsNRAMP2 contribute to Cd translocation and plant immunity,whereas OsNRAMP3/4/6/7 participate in Mn,iron,and zinc distribution and stress responses.This review systematically summarizes the structural,functional,and regulatory mechanisms of OsNRAMPs,emphasizing their roles in metal transport,pathogen resistance,and abiotic stress adaptation.Furthermore,we discuss strategies for developing low-Cd rice varieties,including QTL-based breeding,CRISPR/Cas9-mediated gene editing,and multi-gene stacking approaches.Finally,we outline future research directions,such as structural engineering of metal-binding sites and field validation of engineered rice lines,to ensure sustainable rice production in heavy metal-contaminated soils.
基金funded by the National Key R&D Program of China (Grant No. 2024YFD2300301)the National Natural Science Foundation of China (Grant Nos. 32472223 and 31901447)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutionsthe Qinglan Project of Jiangsu Province, China
文摘Clarifying the photosynthetic and population characteristics of conventional japonica rice at different yield levels is crucial for boosting yield and ensuring food security.Therefore,a two-year field trial with two conventional japonica varieties was conducted at four planting densities:16 cm×30 cm(D1),14 cm×30 cm(D2),12 cm×30 cm(D3),and 10 cm×30 cm(D4).This study aimed to investigate how photosynthetic and population characteristics influence grain yield under varying planting densities.The results indicated that higher yields were primarily driven by increased grain weight and seed-setting rate(with a 9.68%‒11.40%higher single panicle weight),supported by optimized dry matter translocation and source-sink relationships.Elevated planting density(D2‒D4)enhanced panicle number and total spikelet number(by 3.91%‒15.00%)but reduced the number of spikelets per panicle,1000-grain weight,and photosynthetic efficiency due to mutual shading.Despite these trade-offs,yield increased by 4.10%‒12.42%under higher densities.The use of planting density D4 in japonica rice cultivation contributed to maximize yield.These findings provide important theoretical insights and practical significance for increasing the yield of conventional japonica rice and ensuring food security.
基金financially supported by the National Natural Science Foundation of China (32071978)the National Key Research and Development Program of China (2022YFD2300901 and 2022YFD2300905)。
文摘High temperature stress (HT) significantly reduces maize yield by impairing starch accumulation in kernels.However,the mechanism by which HT affects starch synthesis remains controversial-whether through reduced assimilate supply or direct inhibition on kernel metabolism.To clarify these mechanisms,a heat-sensitive maize hybrid,Xianyu 335 (XY),was exposed to 30℃/20℃ (maximum/minimum temperature,control) and 40℃/30℃ for seven consecutive days during the seed setting stage.Synchronous pollination (SP),apical pollination (AP),and shading treatments were applied to manipulate the inherent source–sink ratio in maize plants.Results showed that apical kernel weight decreased by 11.9%under 40℃ in the SP treatment.The ^(13)C content,starch accumulation,and cell-wall invertase (CWIN) activity also declined by 15.9,36.7,and 16.4%,respectively,under HT.In the shading treatment,40℃/30℃ caused even greater reductions in^(13)C content,starch accumulation,and CWIN activity due to diminished assimilate supply.Conversely,in the AP treatment,starch content and CWIN activity increased by 22.0 and 18.5%,respectively,under 40℃/30℃,resulting in kernel weight and ^(13)C content similar to those in SP and shading treatments regardless of temperature.Consistent with apical kernels under AP,HT did not negatively affect middle kernels in either SP or shading treatments,as kernel weight and starch content remained unchanged under HT.Although all kernels were exposed to the same HT or control environment,their responses varied a lot.The impaired starch synthesis in apical kernels under HT was rescued by increasing carbon supply via AP treatment.The contrasting performance among middle kernels,apical kernels under AP,and apical kernels under SP or shading indicates that reduced carbon supply is a critical factor underlying inhibited starch accumulation.Our findings provide a theoretical basis for further understanding kernel abortion under HT.
基金supported by the key project of National Natural Sciences Foundation of China(U22A20551,32030085)the Major Project of Hubei Hongshan Laboratory,China(2021hszd015)+2 种基金the Hubei Province Major Science and Technology Special Project,China(2023BBA002)the National Natural Sciences Foundation of China(U22A20551)the National Natural Science Foundation of China Excellent Youth Fund(32422072)。
文摘Aspergillus species are ubiquitous fungi that produce mycotoxins(secondary metabolites)known as sterigmatocystin and aflatoxins in many different kinds of foods,which leads to serious contamination in agricultural products,thereby endangering human health.Extensive studies on Aspergillus fungi have been conducted on growth and development,aflatoxin biosynthesis,and their interactions with environment.Here,we summarized a series of functional genes of the main Aspergillus fungi relative to toxins occurrence in foods,which revealed the signal transduction mechanisms of their involvement in growth and development,toxin production,and response to light,anticipating providing theoretical guidance on developing control and prevention technologies for mycotoxin contamination in agricultural products to ensure food safety.
基金supported by the Agricultural Science and Technology Innovation Program of CAAS,the China Agriculture Research System(CARS-13)
文摘Rapeseed(Brassica napus L.) is the largest oilseed crop in China and accounts for about 20% of world production.For the last 10 years,the production,planting area,and yield of rapeseed have been stable,with improvement of seed quality and especially seed oil content.China is among the leading countries in rapeseed genomic research internationally,having jointly with other countries accomplished the whole genome sequencing of rapeseed and its two parental species,Brassica oleracea and Brassica rapa.Progress on functional genomics including the identification of QTL governing important agronomic traits such as yield,seed oil content,fertility regulation,disease and insect resistance,abiotic stress,nutrition use efficiency,and pod shattering resistance has been achieved.As a consequence,molecular markers have been developed and used in breeding programs.During 2005–2014,215 rapeseed varieties were registered nationally,including 210 winter-and 5 spring-type varieties.Mechanization across the whole process of rapeseed production was investigated and operating instructions for all relevant techniques were published.Modern techniques for rapeseed field management such as high-density planting,controlled-release fertilizer,and biocontrol of disease and pests combined with precision tools such as drones have been developed and are being adopted in China.With the application of advanced breeding and production technologies,in the near future,the oil yield and quality of rapeseed varieties will be greatly increased,and more varieties with desirable traits,especially early maturation,high yield,high resistance to biotic and abiotic stress,and suitability for mechanized harvesting will be developed.Application of modern technologies on the mechanized management of rapeseed will greatly increase grower profit.
基金supported by the National Natural Science Foundation of China(No.31700316)the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(Group No.118)+2 种基金the Earmarked Fund for China Agriculture Research System(CARS-12)the Fundamental Research Funds for Central Non-Profit Scientific Institution(1610172018009)Graduate School of Chinese Academy of Agricultural Sciences
文摘Sequence-specific nucleases(SSN) that generate double-stranded DNA breaks(DSBs) in genes of interest are the key to site-specific genome editing in plants. Genome editing has developed into one method of reducing undesirable traits in crops by the induction of knockout mutations. Different SSN-mediated genome-editing systems, including LAGLIDADG homing endonucleases or meganucleases, zinc-finger nucleases, transcription activator-like effector nucleases and clustered regularly interspaced short palindromic repeats, are emerging as robust tools for introducing functional mutations in polyploid crops including citrus, wheat, cotton, soybean, rapeseed, potato, grapes, Camelina sativa,dandelion, and tobacco. The approach utilizes knowledge of biological mechanisms for targeted induction of DSBs and their error-prone repair, allowing highly specific changes at designated genome loci. In this review, we briefly describe genome-editing technologies and their application to genetic improvement of polyploid crops.
基金supported by the grants from the Ministry of Agriculture, Czech Republic from projects QJ1230159 (50%) and RO0415 (50%)
文摘Wheat streak mosaic virus（WSMV）has become a re-emerging pathogen in cereal crops in the Czech Republic.WSMV was first reported in the former Czechoslovakia in the early 1980s,and then no record of the virus was documented until 2009.The incidence of the virus was recorded in recent years in several winter wheat fields and many grass species.Here,we surveyed the incidence of WSMV in cereal crops.The results demonstrated the existence of the virus in winter wheat and volunteer wheat during each year of the monitoring period,which spanned from 2013–2016.Although the range of infected samples was low（6.4%of the total tested samples）,a high incidence of well-distributed virus was recorded.In at least six fields,the virus reached severe and potentially epidemic levels.In accordance with our previous report detailing WSMV infection of native grasses,we tested several grass species commonly grown in the Czech Republic.We found that some grass species acted as experimental hosts and possible reservoirs of the virus;these included Anthoxanthum odoratum（sweet vernal grass）,Arrhenatherum elatius（false oat-grass）,Lolium multiflorum（Italian rye-grass）,Bromus japonicus（Japanese chess）,Echinochloa crus-galli（barnyard grass）,Holcus lanatus（meadow soft grass）and Holcus mollis（creeping soft grass）.Some of these grass species are also important weeds of cereals,which may be the potential source of WSMV infection in cereal crops.Several widely used winter wheat cultivars were tested in the field after artificial inoculation with WSMV to evaluate virus titre by RT-qPCR.Overall,the tested cultivars had a low virus titre,which is associated with mild disease symptoms and may provide a good level of crop resistance to WSMV.
基金the National Natural Science Foundation of China(U20A2034 and 32070217)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ZDRW202105 and CAASASTIP-2013-OCRI)。
文摘To address the global demand for rapeseed while considering farmers’profit,we face the challenges of making a quantum leap in seed yield and,at the same time,reducing yield loss due to biotic and abiotic stresses.We also face the challenge of efficiently applying new transformative biotechnology tools such as gene editing and breeding by genome design to increase rapeseed productivity and profitability.In this Perspective,we review advances in research on the physiological and genetic bases of both stress factorsaffected yield stability and seed yield potential,focusing on source–sink relationships and allocation of photosynthetic assimilates to vegetative growth and seed development.We propose research directions and highlight the role of plant architecture in the relative contributions of the root system,leaves,and pods to seed yield.We call for de novo design of new rapeseed crops.We review trait variation in existing germplasm and biotechnologies available for crop design.Finally,we discuss opportunities to apply fundamental knowledge and key germplasm to rapeseed production and propose an ideotype for de novo design of future rapeseed cultivars.
基金The National Program for Crop Germplasm Protection of China(19210163)National Natural Science Foundation of China(32172006)+2 种基金The Plant Germplasm Resources Sharing Platform(NICGR2021-016)National Peanut Industry Technology System Construction(CARS-13)Central Scientific Institution Basal Research Fund(CAAS-OCRI-ZDRW-202101)。
文摘Peanut(Arachis hypogaea L.)is an important oil and cash crop in the world.Peanut germplasm collected in China are abundant,which provides important material guarantee for peanut breeding and industrial development.Here,the safe conservation technology and indicators of peanut germplasm resources in the Oil Crops Middleterm Genebank of China were expounded from three processes of storage,monitoring,reproduction and renewal.We summarized and reviewed the situation of conservation and utilization of peanut germplasm resources in the Middle-term Genebank in the past 20 years.The future research direction of peanut resources in the Oil Crops Middle-term Genebank of China is prospected.
文摘Chickpea(Cicer arietinum L.) and pigeonpea [Cajanus cajan L.(Millsp.)] play an important role in mitigating protein malnutrition for millions of poor vegetarians living in regions of the semi-arid tropics. Abiotic stresses such as excess and limited soil moisture(water-logging and drought), heat and chilling(high and low temperature stresses), soil salinity, and acidity are major yield constraints, as these two crops are grown mostly under rainfed conditions in risk-prone marginal and degraded lands with few or no inputs. Losses due to such stresses vary from 30% to 100% depending on their severity. The literature abounds in basic information concerning screening techniques, physiological mechanisms, and genetics of traits associated with resistance/tolerance to abiotic stresses in these two crops. However, the final outcome in terms of resistant/tolerant varieties has been far from satisfactory. This situation calls for improving selection efficiency through precise phenotyping and genotyping under high-throughput controlled conditions using modern tools of genomics. In this review, we suggest that an integrated approach combining advances from genetics, physiology, and biotechnology needs to be used for higher precision and efficiency of breeding programs aimed at improving abiotic stress tolerance in both chickpea and pigeonpea.
基金Supported by Cultivation of New Varieties of Genetically Modified Major Projects (2011ZX08004-005)Soybean Industry Technology System(CARS-04-PS08)
文摘Drought is one of the most important environmental constraints limiting plant growth, development and crop yield. Many drought-inducible genes have been identified by molecular and genomic analyses in Arabidopsis, rice and other crops. To better understand reaction mechanism of plant to drought tolerance, we mainly focused on introducing the research of transcription factors （TFs） in signal transduction and regulatory network of gene expression conferring drought. A TF could bind multiple target genes to increase one or more kinds of stress tolerance. Sometimes, several TFs might act together with a target gene. So drought-tolerance genes or TFs might respond to high-salinity, cold or other stresses. The crosstalk of multiple stresses signal pathways is a crucial aspect of understanding stress signaling.
基金supported by funds from the National Natural Science Foundation of China(31701346)Fundamental Research Funds for Central Non-profit Scientific Institution(1610172018001)+1 种基金National Transgenic Project of China(2016ZX08004-005)Agricultural Science and Technology Innovation Program of CAAS(CAAS-ASTIP-2016OCRI)。
文摘Soybean(Glycine max)is one of the most important economic legume crops with largest planting area,and is also an important oil crop,as well as food and feed material.Soybean-rhizobia symbiosis plays important roles in plant cultivation and fertilizer application.However,there are many problems in agricultural application of soybean symbiotic nitrogen fixation.In this review,we summarized three restriction factors(host specificity,low nitrogen fixation efficiency and abiotic stress)and discussed research progresses of these factors.Clarification of host specific mechanism will help to select and apply rhizobia inoculants.Both maintaining high nitrogenase activity and delaying nodule senescence can improve the efficiency of symbiotic nitrogen fixation.Abiotic stress-tolerant rhizobia can improve the abiotic stress tolerance of soybean.Breeding stress tolerant genotypes of soybean and rhizobia,obtaining correlated genes are the common strategies to improve soybean symbiotic nitrogen fixation under extreme conditions.Regulatory mechanisms of these restriction factors are still poorly understood and needs further clarification.
基金the Agricultural Research Center,Giza,Egypt,and the Taif University Researchers Supporting Project(TURSP-2020/111),Taif University,Taif,Saudi Arabia for the technical and financial support to this research。
文摘Among the crop production factors,preceding crop and tillage management affect the sustainable use of soil resources and ultimately crop growth and productivity.This study aimed at investigating the impact of preceding winter crops(grass or legume)and different tillage systems on forage yield,quality and nutritive values of three summer grass(Sudan grass,pearl millet and teosinte)and two legume forage crops(cowpea and guar)under arid conditions.The results exhibited that growing forage crops after legumes(as berseem clover)produced the highest fresh and dry forage yields and quality attributes compared with grasses(as wheat)with the exception of crude fiber content,which was decreased.Moreover,tillage practices showed positive impact on forage yields and quality attributes.The maximum forage yields and quality parameters were recorded under conventional tillage(CT)practice compared with reduced tillage(RT)and no-tillage(NT)systems.Among the evaluated crops,the highest yields of fresh forage,dry forage,crude fiber,crude protein and total digestible nutrient were exhibited by grass forage crops(Sudan grass,pearl millet and teosinte),whereas the highest crude protein content and the digestible energy values were produced by legume forage crops(cowpea and guar).The maximum fresh forage,dry forage,crude fiber,crude protein,total digestible nutrient and digestible crude protein yields were produced by pearl millet followed by Sudan grass under CT and RT after berseem clover.The highest net return was recorded by sowing pearl millet after berseem clover and applying CT followed by RT practices,which could be recommended for the commercial production.Moreover,it could be assumed that the combination of growing grass forage crops after legume crops under CT or RT systems could enhance forage crop yield and quality with an improvement in soil properties for sustainable agriculture with low cost and the highest net income.
文摘The basic question is whether our contemporary methods really describe exactly the “plant world” around us, experiments or if it is the result of the current “imperfect” level of pieces of knowledge. Frankly, experiments are often burdened with many errors in sampling, in the selection of experimental plants, at the simulation of environmental stress, in light composition and lighting methods, shortly by a lot of mistakes. Experimental conditions of individual authors in evaluating the same issue often differ—that is to say, results are difficult to compare. The artificial experimental environment should simulate selected average external conditions of a given crop. Very important is the identification of important growth phases and traits that are suitable for the evaluation of the main experimental task. Bad results and also conclusions of experiments can be by either ignorance of these facts or by incorrectly prepared experiments. Very important is also a knowledge level of concerning tested problematic. The main goal is not only to point out the need for measuring important properties of plants in a physiologically correct stage of growth and development, by suitable methods but also on the influence of the properties of utilized seeds in experiments that they can modify plant growth and development. It is often forgotten that impact of stress on the seeds’ properties and through the seed traits on the plant properties, for example, the same variety in of different provenance may give different results, especially influence on the roots, because plant roots are the most sensitive part of plants. Only the different origins of used seeds in identical experiments at two workplaces can significantly change the results of every repeated standard experiment.
文摘As the core of the management of agricultural institutes,scientific research management plays a vital role in strategic development,and also affects the strategic development of agricultural research institutes. This paper expounds the connotation and characteristics of agricultural scientific research management as well as management ability requirements of management personnel. This paper puts forward the recommendations for improving agricultural scientific research management from the view of management personnel.
文摘Peanut or groundnut （ Arachis hypogaea L. ） is an important source of vegetable oil in the world. Genetic enhancement for high yield and high oil content has greatly contributed to enhanced pro-ductivity of peanut and increased supply of peanut oil. Further improving oil content and quality of peanut is still crucial for increasing productivity of arable land and market competitiveness of peanut oil. Based on investigation among the peanut germplasm accessions including wild Arachis species, the oil content could be as high as 65%. Heterosis has been observed for oil content in hybrids derived from diverse crossing parents. Segregates with enhanced oil content have been obtained by pyramiding different genes or alleles with major and minor additive effects. Improved testing techniques for oil content in breeding lines with reduced cost have accelerated breeding progress for high oil content. SSR markers associated with oil content have been identified by association and linkage analysis. The stability of oil content in peanut across seasons and locations is highly associated with cold tolerance, high nutrition efficiency and drought tolerance. Recent progress on improving fatty acids in particular marker assisted backcrossing breeding has contributed to higher quality peanut oil and other products. High oil peanut lines with im-proved resistance to aflatoxin production have been developed. The wild Arachis species would be of great value for peanut breeding in increasing oil content.
基金Supported by the Special Project for Innovation-driven Development of Guangxi(Guike AA17204037)Major Science and Technology Project of Guangxi(Guike AA16380017)Team Project of Guangxi Academy of Agricultural Sciences(2015YT60)
文摘The basic principle of Fenlong technology is to make full use of the cultivated layer and the below soil resources and natural precipitation. It can increase the amount of loosen soil by 1-2 times, water and oxygen by 1 time, and available nutrients by 10%-30%, surface air humidity by more than 10%, crop net photosynthetic efficiency by 10%-30%, biomass by 20%-30%, crop yield by 10%-50%, and crop quality by 5%, and decrease salt content by 20%-40% and the emission of methane and other gases by 10%. In this study, it is firstly proposed to establish a new research field——"Fenlong science of natural resources"(referred to as "Fenlong science"), and the research contents include Fenlong-based sciences of modern agricultural machinery and equipment for full-layer ploughing and bottom ploughing, tillage, crop cultivation, utilization of water resources (natural precipitation, farmland water conservancy, groundwater resources, etc.), conversion and utilization of saline-alkali land, arable degraded grassland reconstruction, ecological reconstruction of desertification land, ecological environment, climate change, economic and social development. It clarifies theory and enriches technology, and provides a major platform for carrying global population developing from more than 7 billion to 10 billion and helping people and nature to mutually benefit.
