Objective:To explore a rapid and accurate method for evaluating the quality of Prunus mandshurica(Maxim.)Koehne(P.mandshurica,Ku Xing Ren)during rancidity using machine vision and learning.Methods:Sensory evaluation a...Objective:To explore a rapid and accurate method for evaluating the quality of Prunus mandshurica(Maxim.)Koehne(P.mandshurica,Ku Xing Ren)during rancidity using machine vision and learning.Methods:Sensory evaluation and chemometrics were used to classify P.mandshurica quality grades after rancidity.Chemical indicators of the P.mandshurica quality change were determined to verify the ob-tained grades and support the subsequent modeling.The International Commission on Illumination color space was used to extract the color features of the P.mandshurica.Discrimination and prediction models based on color features combined with multiple machine learning algorithms were established using 10-fold cross-validation and external test set validation.Results:The P.mandshurica rancidity samples were allocated to three quality grades.The Bayes net model based on powder color successfully identified the P.mandshurica at different grades with an accuracy of 88.89%and 100%using two validations,and the naive Bayes model based on section color achieved the same accuracy with an receiver operating characteristic area of 0.979.The instance-based k-nearest neighbors model based on powder color performed best in predicting the amygdalin content[R^(2)=0.9801,mean absolute error(MAE)=0.2071,root mean squared error(RMSE)=0.4170],followed by the random com-mittee model in predicting the acid value(R^(2)=0.9580,MAE=1.5121,RMSE=1.9099)and the random forest model in predicting the peroxide value(R^(2)=0.8857,MAE=0.0027,RMSE=0.0035).Conclusion:This study demonstrates that color digitization analysis is a potential method for rapidly evaluating the quality of P.mandshurica across the rancidity process,providing a new reference for the quality assessment of traditional Chinese medicines.展开更多
In sub-Saharan Africa,limited studies have investigated zoonotic pathogens that may be harboured by ticks infesting reptiles such as tortoises.Here,we report the presence of pathogenic Rickettsia in ticks(Amblyomma ma...In sub-Saharan Africa,limited studies have investigated zoonotic pathogens that may be harboured by ticks infesting reptiles such as tortoises.Here,we report the presence of pathogenic Rickettsia in ticks(Amblyomma marmoreum)collected from the leopard tortoise(Geochelone pardalis)in rural Zambia.Using polymerase chain reaction,56%(49/87)of ticks were positive for the Rickettsia outer membrane protein(ompB)gene.Multi-locus sequence and phylogenetic analysis based on the ompB,ompA,and citrate synthase(gltA)genes showed that the ticks carried R.africae,and other Rickettsia spp.closely related to R.raoultii,R.massiliae,R.tamurae and R.monacensis.Given the proximity between humans,livestock,and wildlife in these habitats,there exists a considerable risk of transmission of zoonotic Rickettsia to human populations in this rural setting.These results call for heightened awareness and further research into the dynamics of tick-borne diseases in regions where humans and animals coexist,particularly in the context of tortoise-associated ticks as vectors.Understanding and addressing these potential disease vectors is crucial for effective public health measures and the prevention of Rickettsia zoonoses.展开更多
Unilateral or bilateral perineal hernia results from the failure of the muscular pelvic diaphragm,and is commonly observed in male dogs.This study aimed to investigate the outcome of herniorrhaphy technique with the c...Unilateral or bilateral perineal hernia results from the failure of the muscular pelvic diaphragm,and is commonly observed in male dogs.This study aimed to investigate the outcome of herniorrhaphy technique with the cone-shaped polypropylene mesh in dogs with perineal hernia.1 Materials and Methods Between October 2008 and July 2012,39 dogs(71 perineal hernias)underwent herniorrhaphy with polypropylene mesh(PROLENE MeshJohnson&Johnson)formed in the shape of a cone.The apex of corn-shaped mesh was inserted into the hernia region,and the bottom was sutured to sacrotuberous ligament,internal obturator muscle,external anal sphincter and residual coccygeal muscle.展开更多
Postoperative prognosis is generally poor even with adjuvant chemotherapy in dogs with an appendicular osteosarcoma. The purpose of this report was to present the long-term disease-free survival time in a dog with app...Postoperative prognosis is generally poor even with adjuvant chemotherapy in dogs with an appendicular osteosarcoma. The purpose of this report was to present the long-term disease-free survival time in a dog with appendicular osteosarcoma receiving amputation,adjuvant chemotherapy and adoptive immunotherapy. A 12-year-old spayed Golden retriever was referred to our hospital with a suspicion of osteosarcoma in right distal radius. The patient underwent the computed tomography (CT) and incisional biopsy on the next day after the first presentation. The CT revealed the osteolysis in the right distal radius and no metastases in the lungs and lymph nodes. The histopathologic diagnosis was made as an osteosarcoma. On the 13th day after,the patient underwent right forelimb amputation. Six doses of carboplatin (250 mg / m2) were postoperatively administered every 3 weeks. The intravenous infusion of lymphokine-activated killer (LAK) cells suspension was performed every 3 ~ 4 weeks after the adjuvant chemotherapy. At the time of our writing this abstract (on the 880thday after the first presentation),no recurrences and metastases were observed after the 32 transfers of LAK cells. In canine appendicular osteosarcoma,some reports have described that postoperative median survival times were 235 ~ 540 d with adjuvant chemotherapy. In our report,the disease free interval and survival time were prolonged compared with the previous reports. We strongly believed that early diagnosis and surgical intervention and adoptive immunotherapy had a great impact on the improvement of survival time in canine appendicular osteosarcoma.展开更多
Thymoma is an intrathoracic malignant tumor occurring rarely in cats. Although surgery is the treatment of choice in feline thymomas,complete resection may be difficult in cases that the large vessels are involved int...Thymoma is an intrathoracic malignant tumor occurring rarely in cats. Although surgery is the treatment of choice in feline thymomas,complete resection may be difficult in cases that the large vessels are involved into the mass.Therefore,diagnostic imaging techniques including computed tomography( CT) are essential for the treatment of feline thymomas. This report aimed to present the CT characteristics and successful removal of very large thymoma in a cat with exfoliative dermatitis.An 8-year-old spayed mongrel cat was referred to our hospital with chief complaints of chronic anorexia,alopecia,and desquamation. Tachypnea,tachycardia,and exfoliative dermatitis were observed in the first presentation. Thoracic radiography showed a very large mass in the craniomediastinal region. In the CT findings,the mass included multiple small cysts,and forced up the intrathoracic trachea,cranial vena cava,brachiocephalic trunk and left subclavian artery as well as heart and lungs.On the 6th day after,the patient underwent a median sternotomy and diaphragmatic incision to expose the mass. The mass was completely resected with right phrenic nerve by using cautery and LigaSure. No postoperative complications were observed. The histopathologic diagnosis was made as a thymoma. Postoperative progress of the patient was excellent without the exfoliative dermatitis.Extremely enlarged thymoma could be completely resected in this patient,and the exfoliative dermatitis was postoperatively controlled. The paraneoplastic immune disorder was suggested to cause the dermatitis. CT has a great impact on the indication of surgery for the feline thymomas.展开更多
Hepatocellular carcinoma (HCC) is generally observed as a massive tumor originated from one lobe in dogs.Surgery is the treatment of choice in dogs with a massive HCC. However,complete resection is technically difficu...Hepatocellular carcinoma (HCC) is generally observed as a massive tumor originated from one lobe in dogs.Surgery is the treatment of choice in dogs with a massive HCC. However,complete resection is technically difficult in the cases with an extremely enlarged HCC pressing or involving the large vessels such as caudal vena cava and main hepatic veins. This study aimed to report the divisional hepatic lobectomy for complete resection of very large HCC and successful treatment of paraneoplastic hypoglycemic seizures in a dog.A 12-year-old spayed Yorkshire terrier was referred to our hospital with chief complaints of seizures and a very large hepatic mass. In the first presentation,serum liver enzymes levels increased,and serum glucose concentration was 29mg/dl. Computed tomography (CT) angiography revealed that a huge mass occurred in the right medial,quadrate,and left medial hepatic lobes and flattened caudal vena cava and main portal vein. The patient underwent surgical treatment.The mass was approached with midline celiotomy,caudal median sternotomy,and bilateral paracostal laparotomy. En bloc resection of central and left divisional lobes including the mass was accomplished with surgical ligation of hepatic hilar vessels and hepatic veins. The hypoglycemic seizures were postoperatively resolved,and no clinical signs were observed.The hypoglycemic seizures were thought to be associated with the insulin-like growth factors (IGF). The right divisional lobes could serve the sufficient hepatic function. Divisional hepatic lobectomy based on CT angiography is very useful for the treatment of canine HCCs.展开更多
基金funded by the National Natural Science Foundation of China(81573542)Shanxi Province's Traditional Chinese Medicine Technology Innovation Project(2100601).
文摘Objective:To explore a rapid and accurate method for evaluating the quality of Prunus mandshurica(Maxim.)Koehne(P.mandshurica,Ku Xing Ren)during rancidity using machine vision and learning.Methods:Sensory evaluation and chemometrics were used to classify P.mandshurica quality grades after rancidity.Chemical indicators of the P.mandshurica quality change were determined to verify the ob-tained grades and support the subsequent modeling.The International Commission on Illumination color space was used to extract the color features of the P.mandshurica.Discrimination and prediction models based on color features combined with multiple machine learning algorithms were established using 10-fold cross-validation and external test set validation.Results:The P.mandshurica rancidity samples were allocated to three quality grades.The Bayes net model based on powder color successfully identified the P.mandshurica at different grades with an accuracy of 88.89%and 100%using two validations,and the naive Bayes model based on section color achieved the same accuracy with an receiver operating characteristic area of 0.979.The instance-based k-nearest neighbors model based on powder color performed best in predicting the amygdalin content[R^(2)=0.9801,mean absolute error(MAE)=0.2071,root mean squared error(RMSE)=0.4170],followed by the random com-mittee model in predicting the acid value(R^(2)=0.9580,MAE=1.5121,RMSE=1.9099)and the random forest model in predicting the peroxide value(R^(2)=0.8857,MAE=0.0027,RMSE=0.0035).Conclusion:This study demonstrates that color digitization analysis is a potential method for rapidly evaluating the quality of P.mandshurica across the rancidity process,providing a new reference for the quality assessment of traditional Chinese medicines.
文摘In sub-Saharan Africa,limited studies have investigated zoonotic pathogens that may be harboured by ticks infesting reptiles such as tortoises.Here,we report the presence of pathogenic Rickettsia in ticks(Amblyomma marmoreum)collected from the leopard tortoise(Geochelone pardalis)in rural Zambia.Using polymerase chain reaction,56%(49/87)of ticks were positive for the Rickettsia outer membrane protein(ompB)gene.Multi-locus sequence and phylogenetic analysis based on the ompB,ompA,and citrate synthase(gltA)genes showed that the ticks carried R.africae,and other Rickettsia spp.closely related to R.raoultii,R.massiliae,R.tamurae and R.monacensis.Given the proximity between humans,livestock,and wildlife in these habitats,there exists a considerable risk of transmission of zoonotic Rickettsia to human populations in this rural setting.These results call for heightened awareness and further research into the dynamics of tick-borne diseases in regions where humans and animals coexist,particularly in the context of tortoise-associated ticks as vectors.Understanding and addressing these potential disease vectors is crucial for effective public health measures and the prevention of Rickettsia zoonoses.
文摘Unilateral or bilateral perineal hernia results from the failure of the muscular pelvic diaphragm,and is commonly observed in male dogs.This study aimed to investigate the outcome of herniorrhaphy technique with the cone-shaped polypropylene mesh in dogs with perineal hernia.1 Materials and Methods Between October 2008 and July 2012,39 dogs(71 perineal hernias)underwent herniorrhaphy with polypropylene mesh(PROLENE MeshJohnson&Johnson)formed in the shape of a cone.The apex of corn-shaped mesh was inserted into the hernia region,and the bottom was sutured to sacrotuberous ligament,internal obturator muscle,external anal sphincter and residual coccygeal muscle.
文摘Postoperative prognosis is generally poor even with adjuvant chemotherapy in dogs with an appendicular osteosarcoma. The purpose of this report was to present the long-term disease-free survival time in a dog with appendicular osteosarcoma receiving amputation,adjuvant chemotherapy and adoptive immunotherapy. A 12-year-old spayed Golden retriever was referred to our hospital with a suspicion of osteosarcoma in right distal radius. The patient underwent the computed tomography (CT) and incisional biopsy on the next day after the first presentation. The CT revealed the osteolysis in the right distal radius and no metastases in the lungs and lymph nodes. The histopathologic diagnosis was made as an osteosarcoma. On the 13th day after,the patient underwent right forelimb amputation. Six doses of carboplatin (250 mg / m2) were postoperatively administered every 3 weeks. The intravenous infusion of lymphokine-activated killer (LAK) cells suspension was performed every 3 ~ 4 weeks after the adjuvant chemotherapy. At the time of our writing this abstract (on the 880thday after the first presentation),no recurrences and metastases were observed after the 32 transfers of LAK cells. In canine appendicular osteosarcoma,some reports have described that postoperative median survival times were 235 ~ 540 d with adjuvant chemotherapy. In our report,the disease free interval and survival time were prolonged compared with the previous reports. We strongly believed that early diagnosis and surgical intervention and adoptive immunotherapy had a great impact on the improvement of survival time in canine appendicular osteosarcoma.
文摘Thymoma is an intrathoracic malignant tumor occurring rarely in cats. Although surgery is the treatment of choice in feline thymomas,complete resection may be difficult in cases that the large vessels are involved into the mass.Therefore,diagnostic imaging techniques including computed tomography( CT) are essential for the treatment of feline thymomas. This report aimed to present the CT characteristics and successful removal of very large thymoma in a cat with exfoliative dermatitis.An 8-year-old spayed mongrel cat was referred to our hospital with chief complaints of chronic anorexia,alopecia,and desquamation. Tachypnea,tachycardia,and exfoliative dermatitis were observed in the first presentation. Thoracic radiography showed a very large mass in the craniomediastinal region. In the CT findings,the mass included multiple small cysts,and forced up the intrathoracic trachea,cranial vena cava,brachiocephalic trunk and left subclavian artery as well as heart and lungs.On the 6th day after,the patient underwent a median sternotomy and diaphragmatic incision to expose the mass. The mass was completely resected with right phrenic nerve by using cautery and LigaSure. No postoperative complications were observed. The histopathologic diagnosis was made as a thymoma. Postoperative progress of the patient was excellent without the exfoliative dermatitis.Extremely enlarged thymoma could be completely resected in this patient,and the exfoliative dermatitis was postoperatively controlled. The paraneoplastic immune disorder was suggested to cause the dermatitis. CT has a great impact on the indication of surgery for the feline thymomas.
文摘Hepatocellular carcinoma (HCC) is generally observed as a massive tumor originated from one lobe in dogs.Surgery is the treatment of choice in dogs with a massive HCC. However,complete resection is technically difficult in the cases with an extremely enlarged HCC pressing or involving the large vessels such as caudal vena cava and main hepatic veins. This study aimed to report the divisional hepatic lobectomy for complete resection of very large HCC and successful treatment of paraneoplastic hypoglycemic seizures in a dog.A 12-year-old spayed Yorkshire terrier was referred to our hospital with chief complaints of seizures and a very large hepatic mass. In the first presentation,serum liver enzymes levels increased,and serum glucose concentration was 29mg/dl. Computed tomography (CT) angiography revealed that a huge mass occurred in the right medial,quadrate,and left medial hepatic lobes and flattened caudal vena cava and main portal vein. The patient underwent surgical treatment.The mass was approached with midline celiotomy,caudal median sternotomy,and bilateral paracostal laparotomy. En bloc resection of central and left divisional lobes including the mass was accomplished with surgical ligation of hepatic hilar vessels and hepatic veins. The hypoglycemic seizures were postoperatively resolved,and no clinical signs were observed.The hypoglycemic seizures were thought to be associated with the insulin-like growth factors (IGF). The right divisional lobes could serve the sufficient hepatic function. Divisional hepatic lobectomy based on CT angiography is very useful for the treatment of canine HCCs.
