Background:Biochemical and hematological parameters are important tools for assessing the physiological profile of vital organs,and can be recorded to create reference values used for clinical diagnosis of diseases.Ma...Background:Biochemical and hematological parameters are important tools for assessing the physiological profile of vital organs,and can be recorded to create reference values used for clinical diagnosis of diseases.Many research laboratories lack the means to establish their own set of reference parameters for use in their research,and while there are articles in the literature that discuss laboratory parameters for healthy BALB/c mice,few studies address the evaluation of these parameters in pathological situations,such as in mice inoculated with Ehrlich tumor.Method:BALB/c-FMABC mice previously inoculated with Ehrlich tumor were maintained under appropriate conditions.Blood samples were taken for analysis of hematological parameters using automated and semi-automated equipment to create a set of the animal welfare parameters for evaluation.Result:Results were obtained for all the hematological parameters for all groups analyzed.These showed:statistically significant differences between the initial and final tumor weight;comparable initial tumour volume and weight;an increase in leukocytes in the 7-day group with a characteristic predominance of lymphocytes and neutrophils;statistically significant changes in RDW in the 21-day group and in the welfare parameters in the 28-day group.Conclusion:The study successfully defined and established reference values for hematological and welfare parameters for all groups analyzed.展开更多
The pancreaticoduodenectomy(PD) procedure may lead to pancreatic exocrine and endocrine insufficiency.There are several types of reconstruction for this kind of operation.Pancreaticogastrostomy(PG) was introduced to r...The pancreaticoduodenectomy(PD) procedure may lead to pancreatic exocrine and endocrine insufficiency.There are several types of reconstruction for this kind of operation.Pancreaticogastrostomy(PG) was introduced to reduce the rate of postoperative pancreatic fistula.Although some randomized control trials have shown no differences regarding pancreatic leakage between PG and pancreaticojejunostomy(PJ),recently some reports reveal benefits from the PG over the PJ.Some surgeons concern about the performing of the PG and inactivation of pancreatic enzymes being in contact with the gastric juice,and the detrimental results over the exocrine pancreatic function.The pancreatic exocrine function can be measured with direct and indirect tests.Direct tests have the highest sensitivity and specificity for detection of exocrine insufficiency but require tube placement.Among the tubeless indirect tests,the van de Kamer stool fat analysis remains the standard to diagnose fat malabsorption.The patient compliance and time consuming makes it not so suitable for its clinical use.Fecal immunoreactive elastase test is employed for screening of exocrine insufficiency,is not cumbersome,and has been used to study pancreatic function after resection.We analyze the FE1 levels in our patients after the PD with two types of reconstruction,PG and PJ,and we discuss some considerations about the pancreaticointestinal drainage method after pancreaticoduodenectomy.展开更多
BACKGROUND Acute decompensation(AD)of cirrhosis is related to systemic inflammation and elevated circulating cytokines.In this context,biomarkers of inflammation,such as calprotectin,may be of prognostic value.AIM To ...BACKGROUND Acute decompensation(AD)of cirrhosis is related to systemic inflammation and elevated circulating cytokines.In this context,biomarkers of inflammation,such as calprotectin,may be of prognostic value.AIM To evaluate serum calprotectin levels in patients hospitalized for complications of cirrhosis.METHODS This is a prospective cohort study that included 200 subjects hospitalized for complications of cirrhosis,20 outpatients with stable cirrhosis,and 20 healthy controls.Serum calprotectin was measured by enzyme-linked immunosorbant assay.RESULTS Calprotectin levels were higher among groups with cirrhosis when compared to healthy controls.Higher median calprotectin was related to Child-Pugh C,ascites,and hepatic encephalopathy.Higher calprotectin was related to acute-on-chronic liver failure(ACLF)and infection in the bivariate,but not in multivariate analysis.Calprotectin was not associated with survival among patients with ACLF;however,in patients with AD without ACLF,higher calprotectin was associated with a lower 30-d survival,even after adjustment for chronic liver failure-consortium(CLIF-C)AD score.A high-risk group(CLIF-C AD score≥60 and calprotectin≥580 ng/mL)was identified,which had a 30-d survival(27.3%)similar to that of patients with grade 3 ACLF(23.3%).CONCLUSION Serum calprotectin is associated with prognosis in patients with AD without ACLF and may be useful in clinical practice to early identify patients with a very low short-term survival.展开更多
文摘Background:Biochemical and hematological parameters are important tools for assessing the physiological profile of vital organs,and can be recorded to create reference values used for clinical diagnosis of diseases.Many research laboratories lack the means to establish their own set of reference parameters for use in their research,and while there are articles in the literature that discuss laboratory parameters for healthy BALB/c mice,few studies address the evaluation of these parameters in pathological situations,such as in mice inoculated with Ehrlich tumor.Method:BALB/c-FMABC mice previously inoculated with Ehrlich tumor were maintained under appropriate conditions.Blood samples were taken for analysis of hematological parameters using automated and semi-automated equipment to create a set of the animal welfare parameters for evaluation.Result:Results were obtained for all the hematological parameters for all groups analyzed.These showed:statistically significant differences between the initial and final tumor weight;comparable initial tumour volume and weight;an increase in leukocytes in the 7-day group with a characteristic predominance of lymphocytes and neutrophils;statistically significant changes in RDW in the 21-day group and in the welfare parameters in the 28-day group.Conclusion:The study successfully defined and established reference values for hematological and welfare parameters for all groups analyzed.
文摘The pancreaticoduodenectomy(PD) procedure may lead to pancreatic exocrine and endocrine insufficiency.There are several types of reconstruction for this kind of operation.Pancreaticogastrostomy(PG) was introduced to reduce the rate of postoperative pancreatic fistula.Although some randomized control trials have shown no differences regarding pancreatic leakage between PG and pancreaticojejunostomy(PJ),recently some reports reveal benefits from the PG over the PJ.Some surgeons concern about the performing of the PG and inactivation of pancreatic enzymes being in contact with the gastric juice,and the detrimental results over the exocrine pancreatic function.The pancreatic exocrine function can be measured with direct and indirect tests.Direct tests have the highest sensitivity and specificity for detection of exocrine insufficiency but require tube placement.Among the tubeless indirect tests,the van de Kamer stool fat analysis remains the standard to diagnose fat malabsorption.The patient compliance and time consuming makes it not so suitable for its clinical use.Fecal immunoreactive elastase test is employed for screening of exocrine insufficiency,is not cumbersome,and has been used to study pancreatic function after resection.We analyze the FE1 levels in our patients after the PD with two types of reconstruction,PG and PJ,and we discuss some considerations about the pancreaticointestinal drainage method after pancreaticoduodenectomy.
基金Supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico.
文摘BACKGROUND Acute decompensation(AD)of cirrhosis is related to systemic inflammation and elevated circulating cytokines.In this context,biomarkers of inflammation,such as calprotectin,may be of prognostic value.AIM To evaluate serum calprotectin levels in patients hospitalized for complications of cirrhosis.METHODS This is a prospective cohort study that included 200 subjects hospitalized for complications of cirrhosis,20 outpatients with stable cirrhosis,and 20 healthy controls.Serum calprotectin was measured by enzyme-linked immunosorbant assay.RESULTS Calprotectin levels were higher among groups with cirrhosis when compared to healthy controls.Higher median calprotectin was related to Child-Pugh C,ascites,and hepatic encephalopathy.Higher calprotectin was related to acute-on-chronic liver failure(ACLF)and infection in the bivariate,but not in multivariate analysis.Calprotectin was not associated with survival among patients with ACLF;however,in patients with AD without ACLF,higher calprotectin was associated with a lower 30-d survival,even after adjustment for chronic liver failure-consortium(CLIF-C)AD score.A high-risk group(CLIF-C AD score≥60 and calprotectin≥580 ng/mL)was identified,which had a 30-d survival(27.3%)similar to that of patients with grade 3 ACLF(23.3%).CONCLUSION Serum calprotectin is associated with prognosis in patients with AD without ACLF and may be useful in clinical practice to early identify patients with a very low short-term survival.
