This paper deals with the Neumann problem for a class of semilinear elliptic equations -△u + u =|u|2*-2u+ μ|u|q-2u in Ω, au/ar= |u|(?)*-2u on aΩ, where 2 = 2N/N-2, s=2(N-1)/N-2, 1 <q<2,N(?)3,μ>γ denotes...This paper deals with the Neumann problem for a class of semilinear elliptic equations -△u + u =|u|2*-2u+ μ|u|q-2u in Ω, au/ar= |u|(?)*-2u on aΩ, where 2 = 2N/N-2, s=2(N-1)/N-2, 1 <q<2,N(?)3,μ>γ denotes the unit outward normal to boundary aΩ. By vaxiational method and dual fountain theorem, the existence of infinitely many solutions with negative energy is proved.展开更多
The method of boundary layer with multiple scales and computer algebra were applied to study the asymptotic behavior of solution of boundary value problems for a class of system of nonlinear differential equations . T...The method of boundary layer with multiple scales and computer algebra were applied to study the asymptotic behavior of solution of boundary value problems for a class of system of nonlinear differential equations . The asymptotic expansions of solution were constructed. The remainders were estimated. And an example was analysed. It provides a new foreground for the application of the method of boundary layer with multiple scales .展开更多
Let A be a normal class of algebras. In the present paper, we characterize the following four problems for A: for which radical class R, there holds that(1) R(i1∧i2) = R(i1)∧R(i2);(2) R(i1∨i2) = R(i1)∨R(i2);(3) (i...Let A be a normal class of algebras. In the present paper, we characterize the following four problems for A: for which radical class R, there holds that(1) R(i1∧i2) = R(i1)∧R(i2);(2) R(i1∨i2) = R(i1)∨R(i2);(3) (i1∧i2) =i1∧i2;(4) (i1∨i2) = i1∨i2, for arbitrary algebra a∈A and any i1,i2∈La,where j denotes the ideal of a uniquely determined by R(a/j) = j/j?展开更多
In this paper, a class of strongly nonlinear singularly perturbed interior layer problems are considered by the theory of differential inequalities and the corrective theory of interior layer. The existence of solutio...In this paper, a class of strongly nonlinear singularly perturbed interior layer problems are considered by the theory of differential inequalities and the corrective theory of interior layer. The existence of solution is proved and the asymptotic behavior of solution for the boundary value problems are studied. And the satisfying result is obtained.展开更多
In this paper we shall introduce a class of new function spaces. They are generalizations of some Orlicz spaces and Orlicz-Sobolev spaces, and they are derived from the results of the investigation on imbedding relati...In this paper we shall introduce a class of new function spaces. They are generalizations of some Orlicz spaces and Orlicz-Sobolev spaces, and they are derived from the results of the investigation on imbedding relations between some Orlicz-Sobolev spaces.展开更多
Objective:The integration of training in theory and practice across the medical education spectrum is being encouraged to increase student understanding and skills in the sciences.This study aimed to determine the dec...Objective:The integration of training in theory and practice across the medical education spectrum is being encouraged to increase student understanding and skills in the sciences.This study aimed to determine the deciding factors that drive students'perceived advantages in class to improve precision education and the teaching model.Methods:A mixed strategy of an existing flipped classroom(FC)and a case-based learning(CBL)model was conducted in a medical morphology curriculum for 575 postgraduate students.The subjective learning evaluation of the individuals(learning time,engagement,study interest and concentration,and professional integration)was collected and analyzed after FC-CBL model learning.Results:The results from the general evaluation showed promising results of the medical morphology in the FC-CBL model.Students felt more engaged by instructors in person and benefited in terms of time-saving,flexible arrangements,and professional improvement.Our study contributed to the FC-CBL model in Research Design in postgraduate training in 4 categories:1)advancing a guideline of precision teaching according to individual characteristics;2)revealing whether a learning background is needed for a Research Design course to guide setting up a preliminary course;3)understanding the perceived advantages and their interfaces;and 4)barriers and/or improvement to implement the FC-CBL model in the Research Design class,such as a richer description of e-learning and hands-on practice.Conclusion:Undertaking a FC-CBL combined model could be a useful addition to pedagogy for medical morphology learning in postgraduate training.展开更多
In this paper, we will introduce the definition of a class of nonlinear functionals and some examples about it and then offer a theorem of the minimum about a class of nonlinear functionals on the close central ball i...In this paper, we will introduce the definition of a class of nonlinear functionals and some examples about it and then offer a theorem of the minimum about a class of nonlinear functionals on the close central ball in the metric linear space with 'β-degree circular homogeneous' metric.展开更多
<正> Ⅰ.INTRODUCTION The initial value problem of degenerate equation as is well-known, has the so-called discrete phenomena in C~∞ If it is discussed in a class of analytic functions, then the initial value pr...<正> Ⅰ.INTRODUCTION The initial value problem of degenerate equation as is well-known, has the so-called discrete phenomena in C~∞ If it is discussed in a class of analytic functions, then the initial value problem has another strange phenomena, that is, the solution of the equation (p≠0) is determined entirely by only one initial value conditiont.This shows that the degenerate equation展开更多
The concept of probabilistic metric spaces was introduced by Menger in 1942.Based on the similar idea, the concept of random normed spaces (i. e. probabilistie linear normed spaces) was presented by Serstnev.Since the...The concept of probabilistic metric spaces was introduced by Menger in 1942.Based on the similar idea, the concept of random normed spaces (i. e. probabilistie linear normed spaces) was presented by Serstnev.Since then, Bocsan, Dumitrescu and You Zhaoyong et al. have been working in this field. In this paper, based upon the study of a class of probabilistic linear normed spaces: B-spaces, we study random operators, which can be used as one of new展开更多
Model theory is usually regarded as a comparatively abstract branch of mathematical logic. In this paper we will record, along the same line as what was done in [1], another one of our trials of finding new applicatio...Model theory is usually regarded as a comparatively abstract branch of mathematical logic. In this paper we will record, along the same line as what was done in [1], another one of our trials of finding new applications of model-theoretic conclusions to other branches of mathematics, Considering certain residue class rings of the ring I of展开更多
Consider a system of nonlinear wave equations (e)2t-c2i△xui=Fi(u,(e)u,(e)x(e)u) in (0,∞)×(R)3 for i=1,┈,m,where Fi(i=1,┈,m) are smooth functions of degree 2 near the origin of their arguments, and u=(u1,┈,um...Consider a system of nonlinear wave equations (e)2t-c2i△xui=Fi(u,(e)u,(e)x(e)u) in (0,∞)×(R)3 for i=1,┈,m,where Fi(i=1,┈,m) are smooth functions of degree 2 near the origin of their arguments, and u=(u1,┈,um),while (e)u and (e)x(e)u represent the first and (second derivatives of u, respectively. In this paper, the author presents a new class of nonlinearity for which the global existence of small solutions is ensured. For example, global existence of small solutions for((e)2t- c21Δx)u1 = u2((e)tu2) + arbitrary cubic terms,((e)2t - c22Δx)u2=u1((e)tu2) + ((e)tu1)u2 + arbitrary cubic termswill be established, provided that c21 ≠ c22.展开更多
Traditional Chinese medicine(TCM)is an ancient medical system distinctive and effective in treating cancer,depression,coronavirus disease 2019(COVID-19),and other diseases.However,the relatively abstract diagnostic me...Traditional Chinese medicine(TCM)is an ancient medical system distinctive and effective in treating cancer,depression,coronavirus disease 2019(COVID-19),and other diseases.However,the relatively abstract diagnostic methods of TCM lack objective measurement,and the complex mechanisms of action are difficult to comprehend,which hinders the application and internationalization of TCM.Recently,while breakthroughs have been made in utilizing methods such as network pharmacology and virtual screening for TCM research,the rise of machine learning(ML)has significantly enhanced their integration with TCM.This article introduces representative methodological cases in quality control,mechanism research,diagnosis,and treatment processes of TCM,revealing the potential applications of ML technology in TCM.Furthermore,the challenges faced by ML in TCM applications are summarized,and future directions are discussed.展开更多
Applying projection pursuit (PP) techniques, this paper takes L-statistics as projectionindices to construct a class of statistics for multivariate tests. The asymptotic distributions ofthe test statistics under null ...Applying projection pursuit (PP) techniques, this paper takes L-statistics as projectionindices to construct a class of statistics for multivariate tests. The asymptotic distributions ofthe test statistics under null hypothesis are derived theoretically. Some simple applications tolocation and dispersion problems are given.展开更多
Objective Recaticimab(SHR-1209)significantly reduces low-density lipoprotein cholesterol levels.However,its effect on glucose metabolism remains unclear.This study aimed to evaluate its effect on glycemic parameters i...Objective Recaticimab(SHR-1209)significantly reduces low-density lipoprotein cholesterol levels.However,its effect on glucose metabolism remains unclear.This study aimed to evaluate its effect on glycemic parameters in a Chinese population.Methods Recaticimab versus placebo was administered in a 5:1 ratio to 110 hyperlipidemia patients who were followed up for 24 weeks.Glycated hemoglobin(HbA1c)levels were measured at baseline every 12 weeks.Fasting plasma glucose(FPG)levels were measured at baseline at week 1,3,5,8,12,16,20,and 24.Repeated-measures mixed-effects models were used to determine the longitudinal association between reacticimab and FPG and HbA1c levels.Results Among the 81 participants with normal glucose metabolism,HbA1c levels significantly decreased(F=4.568,P=0.036).In the 29 participants with abnormal glucose metabolism,a significant time effect was observed for FPG levels(F=2.492,P=0.016).For participants with normal and abnormal glucose metabolism,no significant group×time interaction effects on FPG or HbA1c levels were identified.Conclusion Recaticimab showed no adverse glycemic effects in participants with normal or abnormal glucose metabolism,indicating its safety in patients with or without diabetes.展开更多
β-elemene,a bioactive compound derived from traditional Chinese medicine(TCM),has been clinically used in cancer therapy.However,its molecular physicochemical properties require further optimization,and its precise a...β-elemene,a bioactive compound derived from traditional Chinese medicine(TCM),has been clinically used in cancer therapy.However,its molecular physicochemical properties require further optimization,and its precise anticancer mechanisms remain unknown.In modern drug development,structure-based drug design(SBDD)has significantly conserved resources,with computer-aided techniques such as molecular docking and molecule generation playing essential roles.A comprehensive review of existing molecular biology studies and virtual docking experiments led to the hypothesis that methyltransferaselike 3(METTL3)may serve as a potential target ofβ-elemene.This discovery establishes a scientific foundation for integrating advanced,rational drug design strategies withβ-elemene to enhance the therapeutic efficacy of TCM.Moreover,current artificial intelligence(AI)-based molecular generation models were examined,focusing on de novo molecular generation and lead optimization models.Their applications in the rational drug design ofβ-elemene were preliminarily explored to identify potential strategies for developing more potent anticancer derivatives by analyzing ligand-receptor interactions.展开更多
Elemene is widely recognized as an effective anti-cancer compound and is routinely administered in Chinese clinical settings for the management of several solid tumors,including non-small cell lung cancer(NSCLC).Howev...Elemene is widely recognized as an effective anti-cancer compound and is routinely administered in Chinese clinical settings for the management of several solid tumors,including non-small cell lung cancer(NSCLC).However,its detailed molecular mechanism has not been adequately demonstrated.In this research,it was demonstrated that elemene effectively curtailed NSCLC growth in the patient-derived xenograft(PDX)model.Mechanistically,employing high-throughput screening techniques and subsequent biochemical validations such as microscale thermophoresis(MST),microRNA-145-5p(miR-145-5p)was pinpointed as a critical target through which elemene exerts its anti-tumor effects.Interestingly,elemene serves as a binding stabilizer for miR-145-5p,demonstrating a strong binding affinity(dissociation constant(KD)=0.39±0.17μg/mL)and preventing its degradation both in vitro and in vivo,while not interfering with the synthesis of the primary microRNA transcripts(pri-miRNAs)and precursor miRNAs(pre-miRNAs).The stabilization of miR-145-5p by elemene resulted in an increased level of this miRNA,subsequently suppressing NSCLC progression through the miR-145-5p/mitogen-activated protein kinase kinase kinase 3(MAP3K3)/nuclear factor kappaB(NF-κB)pathway.Our findings provide a new perspective on revealing the interaction patterns between clinical anti-tumor drugs and miRNAs.展开更多
Microneedles(MNs)have attracted increasing attention as a transdermal delivery system(TDDS)[1].However,traditional volatile Chinese medicines cannot be dissolved in conventional soluble MN materials,such as hyaluronic...Microneedles(MNs)have attracted increasing attention as a transdermal delivery system(TDDS)[1].However,traditional volatile Chinese medicines cannot be dissolved in conventional soluble MN materials,such as hyaluronic acid and chitosan,making it difficult for many traditional Chinese medicine ingredients to be applied to MN.Elemene(ELE)was successfully isolated from Curcuma longa,and has numerous antitumor and curative effects[2].展开更多
As sciatica and low back pain are among the most common medical complaints, many studies have duplicated these conditions in animals. Chronic compression of the dorsal root ganglion （CCD） is one of these models. The...As sciatica and low back pain are among the most common medical complaints, many studies have duplicated these conditions in animals. Chronic compression of the dorsal root ganglion （CCD） is one of these models. The surgery is simple： after exposing the L4/L5 intervertebral foramina, stainless steel rods are implanted unilaterally, one rod for each vertebra, to chronically compress the lumbar dorsal root ganglion （DRG）. Then, CCD can be used to simulate the clinical conditions caused by stenosis, such as a laterally herniated disc or foraminal stenosis. As the intraforaminal implantation of a rod results in neuronal somal hyperexcitability and spontaneous action potentials associated with hyperalgesia, spontaneous pain, and mechanical allodynia, CCD provides an animal model that mimics radicular pain in humans. This review concerns the mechanisms of neuronal hyperexcitability, focusing on various patterns of spontaneous discharge including one possible pain signal for mechanical allodynia - evoked bursting. Also, new data regarding its significant property of maintaining peripheral input are also discussed. Investigations using this animal model will enhance our un-derstanding of the neural mechanisms for low back pain and sciatica. Furthermore, the peripheral location of the DRG fa-cilitates its use as a locus for controlling pain with minimal central effects, in the hope of ultimately uncovering analgesics that block neuropathic pain without influencing physiological pain.展开更多
The aim of this review is to evaluate the effectiveness and safety of acupuncture for poor semen quality in infertile men. We searched for relevant trials registered up to May 2013 in 14 databases. We selected randomi...The aim of this review is to evaluate the effectiveness and safety of acupuncture for poor semen quality in infertile men. We searched for relevant trials registered up to May 2013 in 14 databases. We selected randomized controlled trials （RCTs） that compared acupuncture, with or without additional treatment, against placebo, sham, no treatment, or the same additional treatment. Two reviewers independently performed the study selection, data extraction, risk of bias and reporting quality appraisal. Risk of bias and reporting quality were appraised by the Cochrane risk of bias tool, the consolidated standards of reporting trials and Standards for Reporting Interventions in Clinical Trials of Acupuncture. The outcomes were sperm motility, sperm concentration, pregnancy rate, and adverse events. Pregnancy was defined as a positive pregnancy test. Four RCTs met the eligibility criteria. Acupuncture increased the percentage of sperm with rapid progression （mean difference - 6.35, 95% confidence interval （CI）. 4.38-8.32, P〈 0.00001） and sperm concentration （mean difference - 6.42, 95% CI. 4.91-7.92, P〈 0.00001）, but these two outcomes were substantially heterogeneous among the studies （F = 72% and 58%, respectively）. No differences in pregnancy rate were found between acupuncture and control groups （odds ratio 1.60, 95% CI. 0.70-3.69, P= 0.27, F = 0%）. No participants experienced adverse events. The current evidence showing that acupuncture might improve poor semen quality is insufficient because of the small number of studies, inadequacy of procedures and/or insufficient information for semen analysis, high levels of heterogeneity, high risk of bias, and poor quality of reporting. Further large, well-designed RCTs are required.展开更多
Objective:To discuss the effect of Yaobitong capsule on histomorphology of dorsal root ganglion and on expression of p38 MARK phosphorylation in autologous nucleus pulposus transplantation model of rats.Methods:A tota...Objective:To discuss the effect of Yaobitong capsule on histomorphology of dorsal root ganglion and on expression of p38 MARK phosphorylation in autologous nucleus pulposus transplantation model of rats.Methods:A total of 60 SD rats were randomly divided into the blank group,model group and Yaobitong capsule group,with 20 rats in each group.The animal model of autologous nucleus pulposus transplantation around the lumbar nerve root was built.Three days after the modeling,rats were given the drugs for the first time,while rats in the model group were given the equivalent normal saline.After 30 d of continuous administration,samples were collected from rats.HE staining was performed on the dorsal root ganglion of L4 and L5 spinal cord of rats in each group and the expression of p38 MARK phosphorylation was measured.All data were treated with the statistical analysis.Results:The histological examination showed that the histomorphology of dorsal root ganglion in the Yaobitong capsule group was more significantly improved than the one in the model group,while the results of western blot showed that Yaobitong capsule could significandy inhibit the level of p38 MAPK phosphorylation of dorsal root ganglion cells.Conclusions:Yaobitong capsule can improve the symptoms and nerve radiculopathy of autologous nucleus pulposus transplantation of rats and its mechanism may be associated with its inhibiting effect on the level of p38 MAPK phosphorylation.展开更多
文摘This paper deals with the Neumann problem for a class of semilinear elliptic equations -△u + u =|u|2*-2u+ μ|u|q-2u in Ω, au/ar= |u|(?)*-2u on aΩ, where 2 = 2N/N-2, s=2(N-1)/N-2, 1 <q<2,N(?)3,μ>γ denotes the unit outward normal to boundary aΩ. By vaxiational method and dual fountain theorem, the existence of infinitely many solutions with negative energy is proved.
文摘The method of boundary layer with multiple scales and computer algebra were applied to study the asymptotic behavior of solution of boundary value problems for a class of system of nonlinear differential equations . The asymptotic expansions of solution were constructed. The remainders were estimated. And an example was analysed. It provides a new foreground for the application of the method of boundary layer with multiple scales .
基金The NSF (2024201051) of Liaoning Education Department.
文摘Let A be a normal class of algebras. In the present paper, we characterize the following four problems for A: for which radical class R, there holds that(1) R(i1∧i2) = R(i1)∧R(i2);(2) R(i1∨i2) = R(i1)∨R(i2);(3) (i1∧i2) =i1∧i2;(4) (i1∨i2) = i1∨i2, for arbitrary algebra a∈A and any i1,i2∈La,where j denotes the ideal of a uniquely determined by R(a/j) = j/j?
基金Supported by the National Natural Science Foundation of China(No.10071048)the Zhejiang Education Office(No.20030594)Huzhou Teachers College(No.200302).
文摘In this paper, a class of strongly nonlinear singularly perturbed interior layer problems are considered by the theory of differential inequalities and the corrective theory of interior layer. The existence of solution is proved and the asymptotic behavior of solution for the boundary value problems are studied. And the satisfying result is obtained.
文摘In this paper we shall introduce a class of new function spaces. They are generalizations of some Orlicz spaces and Orlicz-Sobolev spaces, and they are derived from the results of the investigation on imbedding relations between some Orlicz-Sobolev spaces.
基金supported by grants from the Hunan Province Academic Degree and Graduate Education Reform Project(No.2020JGYB028)the National Natural Science Foundation of China(No.81971891,No.82172196,No.81772134)+1 种基金the Key Laboratory of Emergency and Trauma(Hainan Medical University)of the Ministry of Education(No.KLET-202108)the College Students'Innovation and Entrepreneurship Project(No.S20210026020013).
文摘Objective:The integration of training in theory and practice across the medical education spectrum is being encouraged to increase student understanding and skills in the sciences.This study aimed to determine the deciding factors that drive students'perceived advantages in class to improve precision education and the teaching model.Methods:A mixed strategy of an existing flipped classroom(FC)and a case-based learning(CBL)model was conducted in a medical morphology curriculum for 575 postgraduate students.The subjective learning evaluation of the individuals(learning time,engagement,study interest and concentration,and professional integration)was collected and analyzed after FC-CBL model learning.Results:The results from the general evaluation showed promising results of the medical morphology in the FC-CBL model.Students felt more engaged by instructors in person and benefited in terms of time-saving,flexible arrangements,and professional improvement.Our study contributed to the FC-CBL model in Research Design in postgraduate training in 4 categories:1)advancing a guideline of precision teaching according to individual characteristics;2)revealing whether a learning background is needed for a Research Design course to guide setting up a preliminary course;3)understanding the perceived advantages and their interfaces;and 4)barriers and/or improvement to implement the FC-CBL model in the Research Design class,such as a richer description of e-learning and hands-on practice.Conclusion:Undertaking a FC-CBL combined model could be a useful addition to pedagogy for medical morphology learning in postgraduate training.
文摘In this paper, we will introduce the definition of a class of nonlinear functionals and some examples about it and then offer a theorem of the minimum about a class of nonlinear functionals on the close central ball in the metric linear space with 'β-degree circular homogeneous' metric.
文摘<正> Ⅰ.INTRODUCTION The initial value problem of degenerate equation as is well-known, has the so-called discrete phenomena in C~∞ If it is discussed in a class of analytic functions, then the initial value problem has another strange phenomena, that is, the solution of the equation (p≠0) is determined entirely by only one initial value conditiont.This shows that the degenerate equation
文摘The concept of probabilistic metric spaces was introduced by Menger in 1942.Based on the similar idea, the concept of random normed spaces (i. e. probabilistie linear normed spaces) was presented by Serstnev.Since then, Bocsan, Dumitrescu and You Zhaoyong et al. have been working in this field. In this paper, based upon the study of a class of probabilistic linear normed spaces: B-spaces, we study random operators, which can be used as one of new
文摘Model theory is usually regarded as a comparatively abstract branch of mathematical logic. In this paper we will record, along the same line as what was done in [1], another one of our trials of finding new applications of model-theoretic conclusions to other branches of mathematics, Considering certain residue class rings of the ring I of
文摘Consider a system of nonlinear wave equations (e)2t-c2i△xui=Fi(u,(e)u,(e)x(e)u) in (0,∞)×(R)3 for i=1,┈,m,where Fi(i=1,┈,m) are smooth functions of degree 2 near the origin of their arguments, and u=(u1,┈,um),while (e)u and (e)x(e)u represent the first and (second derivatives of u, respectively. In this paper, the author presents a new class of nonlinearity for which the global existence of small solutions is ensured. For example, global existence of small solutions for((e)2t- c21Δx)u1 = u2((e)tu2) + arbitrary cubic terms,((e)2t - c22Δx)u2=u1((e)tu2) + ((e)tu1)u2 + arbitrary cubic termswill be established, provided that c21 ≠ c22.
文摘Traditional Chinese medicine(TCM)is an ancient medical system distinctive and effective in treating cancer,depression,coronavirus disease 2019(COVID-19),and other diseases.However,the relatively abstract diagnostic methods of TCM lack objective measurement,and the complex mechanisms of action are difficult to comprehend,which hinders the application and internationalization of TCM.Recently,while breakthroughs have been made in utilizing methods such as network pharmacology and virtual screening for TCM research,the rise of machine learning(ML)has significantly enhanced their integration with TCM.This article introduces representative methodological cases in quality control,mechanism research,diagnosis,and treatment processes of TCM,revealing the potential applications of ML technology in TCM.Furthermore,the challenges faced by ML in TCM applications are summarized,and future directions are discussed.
基金This project is supported by the National Natural Science Foundation of China
文摘Applying projection pursuit (PP) techniques, this paper takes L-statistics as projectionindices to construct a class of statistics for multivariate tests. The asymptotic distributions ofthe test statistics under null hypothesis are derived theoretically. Some simple applications tolocation and dispersion problems are given.
基金funded by Jiangsu Hengrui Pharmaceutical Co.,Ltd.(formerly,Jiangsu Hengrui Medicine Co.,Ltd.).
文摘Objective Recaticimab(SHR-1209)significantly reduces low-density lipoprotein cholesterol levels.However,its effect on glucose metabolism remains unclear.This study aimed to evaluate its effect on glycemic parameters in a Chinese population.Methods Recaticimab versus placebo was administered in a 5:1 ratio to 110 hyperlipidemia patients who were followed up for 24 weeks.Glycated hemoglobin(HbA1c)levels were measured at baseline every 12 weeks.Fasting plasma glucose(FPG)levels were measured at baseline at week 1,3,5,8,12,16,20,and 24.Repeated-measures mixed-effects models were used to determine the longitudinal association between reacticimab and FPG and HbA1c levels.Results Among the 81 participants with normal glucose metabolism,HbA1c levels significantly decreased(F=4.568,P=0.036).In the 29 participants with abnormal glucose metabolism,a significant time effect was observed for FPG levels(F=2.492,P=0.016).For participants with normal and abnormal glucose metabolism,no significant group×time interaction effects on FPG or HbA1c levels were identified.Conclusion Recaticimab showed no adverse glycemic effects in participants with normal or abnormal glucose metabolism,indicating its safety in patients with or without diabetes.
基金Research Project of Hangzhou Normal University,China(Grant No.:2024JCXK06).
文摘β-elemene,a bioactive compound derived from traditional Chinese medicine(TCM),has been clinically used in cancer therapy.However,its molecular physicochemical properties require further optimization,and its precise anticancer mechanisms remain unknown.In modern drug development,structure-based drug design(SBDD)has significantly conserved resources,with computer-aided techniques such as molecular docking and molecule generation playing essential roles.A comprehensive review of existing molecular biology studies and virtual docking experiments led to the hypothesis that methyltransferaselike 3(METTL3)may serve as a potential target ofβ-elemene.This discovery establishes a scientific foundation for integrating advanced,rational drug design strategies withβ-elemene to enhance the therapeutic efficacy of TCM.Moreover,current artificial intelligence(AI)-based molecular generation models were examined,focusing on de novo molecular generation and lead optimization models.Their applications in the rational drug design ofβ-elemene were preliminarily explored to identify potential strategies for developing more potent anticancer derivatives by analyzing ligand-receptor interactions.
基金supported by the National Natural Science Foundation of China(Grant No.:82225048)the Dalian Science and Technology Leading Talents Project,China(Grant No.:2019RD15)Sanming Project of Medicine in Shenzhen,China(Grant No.:SZZYSM202106004).
文摘Elemene is widely recognized as an effective anti-cancer compound and is routinely administered in Chinese clinical settings for the management of several solid tumors,including non-small cell lung cancer(NSCLC).However,its detailed molecular mechanism has not been adequately demonstrated.In this research,it was demonstrated that elemene effectively curtailed NSCLC growth in the patient-derived xenograft(PDX)model.Mechanistically,employing high-throughput screening techniques and subsequent biochemical validations such as microscale thermophoresis(MST),microRNA-145-5p(miR-145-5p)was pinpointed as a critical target through which elemene exerts its anti-tumor effects.Interestingly,elemene serves as a binding stabilizer for miR-145-5p,demonstrating a strong binding affinity(dissociation constant(KD)=0.39±0.17μg/mL)and preventing its degradation both in vitro and in vivo,while not interfering with the synthesis of the primary microRNA transcripts(pri-miRNAs)and precursor miRNAs(pre-miRNAs).The stabilization of miR-145-5p by elemene resulted in an increased level of this miRNA,subsequently suppressing NSCLC progression through the miR-145-5p/mitogen-activated protein kinase kinase kinase 3(MAP3K3)/nuclear factor kappaB(NF-κB)pathway.Our findings provide a new perspective on revealing the interaction patterns between clinical anti-tumor drugs and miRNAs.
基金supported by Shandong Provincial Natural Science Foundation Innovation and Development Joint Project,China(Grant No.:ZR2021LZY039)Zhejiang Provincial Traditional Chinese Medicine Science and Technology Plan,China(Grant No.:2021ZB184)。
文摘Microneedles(MNs)have attracted increasing attention as a transdermal delivery system(TDDS)[1].However,traditional volatile Chinese medicines cannot be dissolved in conventional soluble MN materials,such as hyaluronic acid and chitosan,making it difficult for many traditional Chinese medicine ingredients to be applied to MN.Elemene(ELE)was successfully isolated from Curcuma longa,and has numerous antitumor and curative effects[2].
基金supported by the National Natural Science Foundation of China(30870829)
文摘As sciatica and low back pain are among the most common medical complaints, many studies have duplicated these conditions in animals. Chronic compression of the dorsal root ganglion （CCD） is one of these models. The surgery is simple： after exposing the L4/L5 intervertebral foramina, stainless steel rods are implanted unilaterally, one rod for each vertebra, to chronically compress the lumbar dorsal root ganglion （DRG）. Then, CCD can be used to simulate the clinical conditions caused by stenosis, such as a laterally herniated disc or foraminal stenosis. As the intraforaminal implantation of a rod results in neuronal somal hyperexcitability and spontaneous action potentials associated with hyperalgesia, spontaneous pain, and mechanical allodynia, CCD provides an animal model that mimics radicular pain in humans. This review concerns the mechanisms of neuronal hyperexcitability, focusing on various patterns of spontaneous discharge including one possible pain signal for mechanical allodynia - evoked bursting. Also, new data regarding its significant property of maintaining peripheral input are also discussed. Investigations using this animal model will enhance our un-derstanding of the neural mechanisms for low back pain and sciatica. Furthermore, the peripheral location of the DRG fa-cilitates its use as a locus for controlling pain with minimal central effects, in the hope of ultimately uncovering analgesics that block neuropathic pain without influencing physiological pain.
文摘The aim of this review is to evaluate the effectiveness and safety of acupuncture for poor semen quality in infertile men. We searched for relevant trials registered up to May 2013 in 14 databases. We selected randomized controlled trials （RCTs） that compared acupuncture, with or without additional treatment, against placebo, sham, no treatment, or the same additional treatment. Two reviewers independently performed the study selection, data extraction, risk of bias and reporting quality appraisal. Risk of bias and reporting quality were appraised by the Cochrane risk of bias tool, the consolidated standards of reporting trials and Standards for Reporting Interventions in Clinical Trials of Acupuncture. The outcomes were sperm motility, sperm concentration, pregnancy rate, and adverse events. Pregnancy was defined as a positive pregnancy test. Four RCTs met the eligibility criteria. Acupuncture increased the percentage of sperm with rapid progression （mean difference - 6.35, 95% confidence interval （CI）. 4.38-8.32, P〈 0.00001） and sperm concentration （mean difference - 6.42, 95% CI. 4.91-7.92, P〈 0.00001）, but these two outcomes were substantially heterogeneous among the studies （F = 72% and 58%, respectively）. No differences in pregnancy rate were found between acupuncture and control groups （odds ratio 1.60, 95% CI. 0.70-3.69, P= 0.27, F = 0%）. No participants experienced adverse events. The current evidence showing that acupuncture might improve poor semen quality is insufficient because of the small number of studies, inadequacy of procedures and/or insufficient information for semen analysis, high levels of heterogeneity, high risk of bias, and poor quality of reporting. Further large, well-designed RCTs are required.
基金supported by Shandong Natural Science Fund(No.:Y2008C147)
文摘Objective:To discuss the effect of Yaobitong capsule on histomorphology of dorsal root ganglion and on expression of p38 MARK phosphorylation in autologous nucleus pulposus transplantation model of rats.Methods:A total of 60 SD rats were randomly divided into the blank group,model group and Yaobitong capsule group,with 20 rats in each group.The animal model of autologous nucleus pulposus transplantation around the lumbar nerve root was built.Three days after the modeling,rats were given the drugs for the first time,while rats in the model group were given the equivalent normal saline.After 30 d of continuous administration,samples were collected from rats.HE staining was performed on the dorsal root ganglion of L4 and L5 spinal cord of rats in each group and the expression of p38 MARK phosphorylation was measured.All data were treated with the statistical analysis.Results:The histological examination showed that the histomorphology of dorsal root ganglion in the Yaobitong capsule group was more significantly improved than the one in the model group,while the results of western blot showed that Yaobitong capsule could significandy inhibit the level of p38 MAPK phosphorylation of dorsal root ganglion cells.Conclusions:Yaobitong capsule can improve the symptoms and nerve radiculopathy of autologous nucleus pulposus transplantation of rats and its mechanism may be associated with its inhibiting effect on the level of p38 MAPK phosphorylation.
