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Integration of Theory and Practice in Medical Morphology Curriculum in Postgraduate Training:A Flipped Classroom and Case-based Learning Exercise 被引量:2
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作者 Xi-min HU Zhi-xin LI +5 位作者 Jing DENG Yang HAN Shuang LU Qi ZHANG Zi-qiang LUO Kun XIONG 《Current Medical Science》 SCIE CAS 2023年第4期741-748,共8页
Objective:The integration of training in theory and practice across the medical education spectrum is being encouraged to increase student understanding and skills in the sciences.This study aimed to determine the dec... Objective:The integration of training in theory and practice across the medical education spectrum is being encouraged to increase student understanding and skills in the sciences.This study aimed to determine the deciding factors that drive students'perceived advantages in class to improve precision education and the teaching model.Methods:A mixed strategy of an existing flipped classroom(FC)and a case-based learning(CBL)model was conducted in a medical morphology curriculum for 575 postgraduate students.The subjective learning evaluation of the individuals(learning time,engagement,study interest and concentration,and professional integration)was collected and analyzed after FC-CBL model learning.Results:The results from the general evaluation showed promising results of the medical morphology in the FC-CBL model.Students felt more engaged by instructors in person and benefited in terms of time-saving,flexible arrangements,and professional improvement.Our study contributed to the FC-CBL model in Research Design in postgraduate training in 4 categories:1)advancing a guideline of precision teaching according to individual characteristics;2)revealing whether a learning background is needed for a Research Design course to guide setting up a preliminary course;3)understanding the perceived advantages and their interfaces;and 4)barriers and/or improvement to implement the FC-CBL model in the Research Design class,such as a richer description of e-learning and hands-on practice.Conclusion:Undertaking a FC-CBL combined model could be a useful addition to pedagogy for medical morphology learning in postgraduate training. 展开更多
关键词 flipped classroom case-based learning medical morphology curriculum research design POSTGRADUATE
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Generalized Szasz Problems in Normal Classes of Algebras
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作者 王尧 牛凤文 《Northeastern Mathematical Journal》 CSCD 2004年第2期225-235,共11页
Let A be a normal class of algebras. In the present paper, we characterize the following four problems for A: for which radical class R, there holds that(1) R(i1∧i2) = R(i1)∧R(i2);(2) R(i1∨i2) = R(i1)∨R(i2);(3) (i... Let A be a normal class of algebras. In the present paper, we characterize the following four problems for A: for which radical class R, there holds that(1) R(i1∧i2) = R(i1)∧R(i2);(2) R(i1∨i2) = R(i1)∨R(i2);(3) (i1∧i2) =i1∧i2;(4) (i1∨i2) = i1∨i2, for arbitrary algebra a∈A and any i1,i2∈La,where j denotes the ideal of a uniquely determined by R(a/j) = j/j? 展开更多
关键词 normal class of algebra LATTICE equivalence relation RADICAL
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NEUMANN PROBLEMS OF A CLASS OF ELLIPTIC EQUATIONS WITH DOUBLY CRITICAL SOBOLEV EXPONENTS 被引量:3
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作者 韩丕功 《Acta Mathematica Scientia》 SCIE CSCD 2004年第4期633-638,共6页
This paper deals with the Neumann problem for a class of semilinear elliptic equations -△u + u =|u|2*-2u+ μ|u|q-2u in Ω, au/ar= |u|(?)*-2u on aΩ, where 2 = 2N/N-2, s=2(N-1)/N-2, 1 <q<2,N(?)3,μ>γ denotes... This paper deals with the Neumann problem for a class of semilinear elliptic equations -△u + u =|u|2*-2u+ μ|u|q-2u in Ω, au/ar= |u|(?)*-2u on aΩ, where 2 = 2N/N-2, s=2(N-1)/N-2, 1 <q<2,N(?)3,μ>γ denotes the unit outward normal to boundary aΩ. By vaxiational method and dual fountain theorem, the existence of infinitely many solutions with negative energy is proved. 展开更多
关键词 Neumann problem semilinear elliptic equation (PS)·c condition critical Sobolev exponent
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COMPUTER COMPUTATION OF THE METHOD OF MULTIPLE SCALES-DIRICHLET PROBLEM FOR A CLASS OF SYSTEM OF NONLINEAR DIFFERENTIAL EQUATIONS
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作者 谢腊兵 江福汝 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 2003年第11期1264-1272,共9页
The method of boundary layer with multiple scales and computer algebra were applied to study the asymptotic behavior of solution of boundary value problems for a class of system of nonlinear differential equations . T... The method of boundary layer with multiple scales and computer algebra were applied to study the asymptotic behavior of solution of boundary value problems for a class of system of nonlinear differential equations . The asymptotic expansions of solution were constructed. The remainders were estimated. And an example was analysed. It provides a new foreground for the application of the method of boundary layer with multiple scales . 展开更多
关键词 system of nonlinear differential equation boundary value problem method of boundary layer with multiple scale computer algebra asymptotic solution
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New Approach to Rock Classification Based on Sparse Representations
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作者 Wognin Joseph Vangah Bi G. Théodore Toa +2 位作者 Alico Nango Jerôme Ouattara Sie Alain Clément 《Open Journal of Applied Sciences》 2024年第1期145-158,共14页
In geology, classification and lithological recognition of rocks plays an important role in the area of oil and gas exploration, mineral exploration and geological analysis. In other fields of activity such as constru... In geology, classification and lithological recognition of rocks plays an important role in the area of oil and gas exploration, mineral exploration and geological analysis. In other fields of activity such as construction and decoration, this classification makes sense and fully plays its role. However, this classification is slow, approximate and subjective. Automatic classification curbs this subjectivity and fills this gap by offering methods that reflect human perception. We propose a new approach to rock classification based on direct-view images of rocks. The aim is to take advantage of feature extraction methods to estimate a rock dictionary. In this work, we have developed a classification method obtained by concatenating four (4) K-SVD variants into a single signature. This method is based on the K-SVD algorithm combined with four (4) feature extraction techniques: DCT, Gabor filters, D-ALBPCSF and G-ALBPCSF, resulting in the four (4) variants named K-Gabor, K-DCT, KD-ALBPCSF and KD-ALBPCSF respectively. In this work, we developed a classification method obtained by concatenating four (4) variants of K-SVD. The performance of our method was evaluated on the basis of performance indicators such as accuracy with other 96% success rate. 展开更多
关键词 Rock Recognition DICTIONARY SIGNATURE Color Texture K-SVD Variants KD-ALBPCSF Et KG-ALBPCSF
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国家文化数字化战略下红色档案活化传播:构成要素、发展趋向与推进策略
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作者 常大伟 王欣歌 《山西档案》 北大核心 2026年第3期17-22,共6页
红色档案是红色精神的重要载体,也是镌刻中国共产党人初心使命最直接的历史凭证。红色档案以贴近人们生活习惯的方式进行传播,能够促进红色档案资源从静态的历史资料转变为动态的、具有生命力和感染力的文化资源。传播主体、传播内容、... 红色档案是红色精神的重要载体,也是镌刻中国共产党人初心使命最直接的历史凭证。红色档案以贴近人们生活习惯的方式进行传播,能够促进红色档案资源从静态的历史资料转变为动态的、具有生命力和感染力的文化资源。传播主体、传播内容、传播媒介、传播受众、传播时机五个要素环环相扣,共同构建起红色档案活化传播的有机整体。随着数字时代信息传播方式的变革,传统单向、静态的传播模式已经难以满足人们日益多元的文化需求,促使红色档案活化传播的理念、场景和使命发生新的转向。为了提升红色档案活化传播的效果,需要从正确把握红色档案活化传播的方向、有效协同红色档案活化传播的主体、不断创新红色档案叙事方式、积极构建红色档案活化传播的融媒体矩阵等方面协同发力,共同推动红色档案从静态保护迈向活态传承。 展开更多
关键词 国家文化数字化战略 红色档案 活化传播 红色档案开发
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ON A NEW CLASS OF FUNCTION SPACES 被引量:1
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作者 丁夏畦 《Chinese Science Bulletin》 SCIE EI CAS 1981年第11期973-976,共4页
In this paper we shall introduce a class of new function spaces. They are generalizations of some Orlicz spaces and Orlicz-Sobolev spaces, and they are derived from the results of the investigation on imbedding relati... In this paper we shall introduce a class of new function spaces. They are generalizations of some Orlicz spaces and Orlicz-Sobolev spaces, and they are derived from the results of the investigation on imbedding relations between some Orlicz-Sobolev spaces. 展开更多
关键词 CLASS notation satis 初云 才人 七子山 otherwise LEMMA BESSEL 江时
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化瘀解毒方对慢性萎缩性胃炎大鼠的保护作用及机制
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作者 于慧敏 李滢滢 +7 位作者 王小杰 崔广晓 冯昆 陈苹苹 张乃霖 刘启泉 耿云云 高维娟 《中国病理生理杂志》 北大核心 2026年第2期328-340,共13页
目的:探究化瘀解毒方对慢性萎缩性胃炎(chronic atrophic gastritis,CAG)大鼠的保护作用及分子机制。方法:采用N-甲基-N'-硝基-N-亚硝基胍(N-methyl-N'-nitro-N-nitrosoguanidine,MNNG)自由饮等综合因素法建立CAG大鼠模型。造... 目的:探究化瘀解毒方对慢性萎缩性胃炎(chronic atrophic gastritis,CAG)大鼠的保护作用及分子机制。方法:采用N-甲基-N'-硝基-N-亚硝基胍(N-methyl-N'-nitro-N-nitrosoguanidine,MNNG)自由饮等综合因素法建立CAG大鼠模型。造模成功后,将大鼠随机分为模型组、胃复春(Weifuchun,WFC)对照组及低、中、高剂量化瘀解毒方组。监测各组大鼠体重、评估胃黏膜状态、检测血清炎症因子水平及胃功能指标。体外构建GES-1恶性转化细胞(malignantly transformed GES-1 cells,MC)模型,采用CCK8法、细胞划痕和Transwell侵袭实验检测BCHR含药血清对MC增殖、迁移和侵袭能力的影响。整合网络药理学预测、分子对接模拟、Western blot和免疫组织化学分析,筛选并验证干预CAG的关键靶点;通过siRNA敲减关键靶点Bcl-2相关转录因子1(Bcl-2-associated transcription factor 1,BTF)或缺氧诱导因子1α(hypoxia-inducible factor-1α,HIF-1α),探究其在CAG中的调控作用。结果:化瘀解毒方干预可显著逆转CAG大鼠体重下降、胃黏膜病理损伤及血清炎症因子和胃功能指标的异常。20%化瘀解毒方含药血清可显著抑制MC细胞增殖、迁移与侵袭能力。网络药理学分析与实验验证结果表明:BTF和HIF-1α是化瘀解毒方干预CAG的关键靶点,化瘀解毒方可显著下调其蛋白表达;敲减BTF或HIF-1α均可下调HIF-1α下游靶基因血管内皮生长因子(vascular endothelial growth factor A,VEGFA)和促红细胞生成素(eryropoietin,EPO)的mRNA表达。结论:化瘀解毒方可有效改善CAG,其保护作用与抑制BTF/HIF-1α信号通路有关。 展开更多
关键词 慢性萎缩性胃炎 胃黏膜损伤 化瘀解毒方 缺氧诱导因子1Α Bcl-2相关转录因子1
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面子倾向对石油销售人员职业倦怠与焦虑的调节效应
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作者 李林玥 朱咏妍 +3 位作者 邹晓琴 李欣潼 石小雷 王永伟 《中华劳动卫生职业病杂志》 北大核心 2026年第1期21-25,共5页
目的揭示石油销售行业职工职业倦怠与焦虑现状,探讨面子倾向对职业倦怠与焦虑的调节效应,为石油销售场所焦虑干预策略的制定提供新依据。方法于2024年5至8月,整群抽取两个石油销售分公司共计1771名一线石油销售人员为研究对象,采用《Mas... 目的揭示石油销售行业职工职业倦怠与焦虑现状,探讨面子倾向对职业倦怠与焦虑的调节效应,为石油销售场所焦虑干预策略的制定提供新依据。方法于2024年5至8月,整群抽取两个石油销售分公司共计1771名一线石油销售人员为研究对象,采用《Maslach倦怠通用版量表》《面子得失量表》《焦虑自评量表》收集其职业倦怠、面子倾向、焦虑等信息。采用Spearman秩相关分析职业倦怠与面子倾向、焦虑的相关关系,采用调节效应模型评估并检验面子倾向对职业倦怠与焦虑的调节效应。结果1771名石油销售人员的职业倦怠得分为(2.07±1.27)分,980人有职业倦怠(占55.3%);面子倾向得分为(32.19±15.77)分;焦虑得分为(46.43±11.49)分,544人检出焦虑(占30.7%)。相关性分析显示,石油销售人员职业倦怠与面子倾向(rs=0.18)、焦虑(rs=0.57)均呈正相关(P<0.001),面子倾向与焦虑呈正相关(rs=0.37,P<0.001)。调节效应模型分析显示,职业倦怠和面子倾向对石油销售人员焦虑均有正向影响(β=4.784、0.157,P<0.001)。石油销售人员面子倾向在职业倦怠与焦虑间起调节作用(△R^(2)=0.049,F=162.48,P<0.001)。结论石油销售人员职业倦怠和焦虑现状不容乐观,面子倾向在职业倦怠导致焦虑中存在调节效应,可通过在工作场所中采取针对性措施帮助职工形成健康的面子认知,从而降低焦虑的发生。 展开更多
关键词 焦虑 职业倦怠 面子倾向 调节效应 石油销售人员
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常见创伤性脑损伤啮齿类动物模型进展
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作者 林嘉僖 谢亦曈 +1 位作者 饶肇滨 杨鹏 《创伤外科杂志》 2026年第1期61-64,共4页
创伤性脑损伤(TBI)是全球范围内导致残疾和死亡的主要病因之一,给家庭和社会带来了沉重的负担。TBI后继发性脑损伤中病理生理机制的探索对于改善治疗和预后有着非常重要的作用,建立更好的TBI动物模型有助于TBI病理生理的进一步探索。啮... 创伤性脑损伤(TBI)是全球范围内导致残疾和死亡的主要病因之一,给家庭和社会带来了沉重的负担。TBI后继发性脑损伤中病理生理机制的探索对于改善治疗和预后有着非常重要的作用,建立更好的TBI动物模型有助于TBI病理生理的进一步探索。啮齿类动物因其体型较小、成本较低等优势而被广泛应用于TBI模型。本文笔者阐述了常见的TBI啮齿类动物模型的相关应用研究,以便在研究中更有针对性地选择相关动物模型。 展开更多
关键词 创伤性脑损伤 动物模型 啮齿类动物 研究进展
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ON THE MINIMUM OF A CLASS OF NONLINEAR FUNCTIONALS
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作者 定光桂 《Chinese Science Bulletin》 SCIE EI CAS 1983年第3期306-309,共4页
In this paper, we will introduce the definition of a class of nonlinear functionals and some examples about it and then offer a theorem of the minimum about a class of nonlinear functionals on the close central ball i... In this paper, we will introduce the definition of a class of nonlinear functionals and some examples about it and then offer a theorem of the minimum about a class of nonlinear functionals on the close central ball in the metric linear space with 'β-degree circular homogeneous' metric. 展开更多
关键词 CLASS CIRCULAR assume SUBSET QUASI mentioned nates finitely 梦夕 ENOUGH
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一种基于HK32单片机的无损探伤控制设备
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作者 袁成营 高宇星 +2 位作者 吴嘉峰 梁小凤 程正乾 《中国集成电路》 2026年第1期61-65,共5页
由于进口处理器存在供货风险,拟在关键设备上采用深圳市航顺芯片技术研发有限公司开发的国产单片机系统芯片HK32F030C8T6,通过交流剩磁法,实现工件磁化退磁控制,以实现无损探伤的互相验证。为了实现闭环控制,在系统加入电流采集环节,使... 由于进口处理器存在供货风险,拟在关键设备上采用深圳市航顺芯片技术研发有限公司开发的国产单片机系统芯片HK32F030C8T6,通过交流剩磁法,实现工件磁化退磁控制,以实现无损探伤的互相验证。为了实现闭环控制,在系统加入电流采集环节,使用美国Cirrus Logic公司的电能计量芯片(CS5490)实现电流检测,将模拟电流转换成串行数字信号,单片机根据电流差值调节相位,控制双向可控硅的导通角,实现了较高精度的控制。 展开更多
关键词 交流剩磁法 HK32单片机 CS5490 闭环
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A CLASS OF COMMUTATIVE RINGS WITH PRIME FORMULAS
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作者 王世强 《Chinese Science Bulletin》 SCIE EI 1983年第4期436-440,共5页
Model theory is usually regarded as a comparatively abstract branch of mathematical logic. In this paper we will record, along the same line as what was done in [1], another one of our trials of finding new applicatio... Model theory is usually regarded as a comparatively abstract branch of mathematical logic. In this paper we will record, along the same line as what was done in [1], another one of our trials of finding new applications of model-theoretic conclusions to other branches of mathematics, Considering certain residue class rings of the ring I of 展开更多
关键词 BRANCHES mathematics INTEGERS CLASS finding conclusions residue record rings STATEMENT
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ON INITIAL VALUE PROBLEM OF A CLASS OF DEGENERATE EQUATION
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作者 郑兴礼 《Chinese Science Bulletin》 SCIE EI CAS 1983年第11期1433-1436,共4页
<正> Ⅰ.INTRODUCTION The initial value problem of degenerate equation as is well-known, has the so-called discrete phenomena in C~∞ If it is discussed in a class of analytic functions, then the initial value pr... <正> Ⅰ.INTRODUCTION The initial value problem of degenerate equation as is well-known, has the so-called discrete phenomena in C~∞ If it is discussed in a class of analytic functions, then the initial value problem has another strange phenomena, that is, the solution of the equation (p≠0) is determined entirely by only one initial value conditiont.This shows that the degenerate equation 展开更多
关键词 DEGENERATE STRANGE ANALYTIC entirely CLASS VANISHING analogous formal uniquely assume
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ULFCB免支模混凝土板力学性能与工程应用研究
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作者 唐习龙 廖强 +1 位作者 赵伟强 陈李新 《中国建材科技》 2026年第1期70-76,共7页
传统木模板存在资源消耗大、碳排放高等问题。本研究提出一种基于超轻质纤维水泥板(Ultra-Lightweight Fiber Cement Board,ULFCB)的免支模永久性模板体系,运用材料性能测试、界面粘结试验、8组四点弯曲试验和有限元模拟的研究方法,考... 传统木模板存在资源消耗大、碳排放高等问题。本研究提出一种基于超轻质纤维水泥板(Ultra-Lightweight Fiber Cement Board,ULFCB)的免支模永久性模板体系,运用材料性能测试、界面粘结试验、8组四点弯曲试验和有限元模拟的研究方法,考察该组合结构力学性能特征,实验数据显示,ULFCB材料力学性能优异,抗拉强度在9.84至11.05MPa之间,抗折强度达14.8至22.5MPa,经表面拉毛开槽工艺处理后,与混凝土的28天面外拉伸粘结强度超过2.13MPa,组合板开裂荷载比传统现浇板提高28%至200%,极限承载力稳定,所建有限元模型预测误差控制在11%以内,指出ULFCB抑制混凝土裂缝的协同工作机制。本研究成果已形成成套技术,并在中山市装配式建筑项目成功应用,验证了该体系在施工便捷性和环保效益方面的优势。 展开更多
关键词 超轻质纤维水泥板(ULFCB) 永久性模板 免支撑施工体系 复合楼板结构
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A CLASS OF PROBABILISTIC LINEAR NORMED SPACES AND RANDOM OPERATORS
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作者 林熙 《Chinese Science Bulletin》 SCIE EI CAS 1983年第10期1295-1298,共4页
The concept of probabilistic metric spaces was introduced by Menger in 1942.Based on the similar idea, the concept of random normed spaces (i. e. probabilistie linear normed spaces) was presented by Serstnev.Since the... The concept of probabilistic metric spaces was introduced by Menger in 1942.Based on the similar idea, the concept of random normed spaces (i. e. probabilistie linear normed spaces) was presented by Serstnev.Since then, Bocsan, Dumitrescu and You Zhaoyong et al. have been working in this field. In this paper, based upon the study of a class of probabilistic linear normed spaces: B-spaces, we study random operators, which can be used as one of new 展开更多
关键词 NORMED operators PROBABILISTIC VALUED CLASS NEIGHBORHOOD UNIQUENESS satis proof briefly
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腹腔镜局部进展期远端胃癌D2根治术标准操作流程:CLASS-01研究共识 被引量:38
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作者 李国新 +1 位作者 季加手 郑民华 《中华胃肠外科杂志》 CAS CSCD 北大核心 2019年第9期807-811,共5页
腹腔镜对比开腹手术治疗局部进展期远端胃癌的前瞻性、多中心、随机对照研究(CLASS-01),为局部进展期胃癌微创外科提供了高级别循证医学证据。结果表明,经验丰富的团队实施腹腔镜远端胃癌D2根治术治疗局部进展期患者是安全、有效的,具... 腹腔镜对比开腹手术治疗局部进展期远端胃癌的前瞻性、多中心、随机对照研究(CLASS-01),为局部进展期胃癌微创外科提供了高级别循证医学证据。结果表明,经验丰富的团队实施腹腔镜远端胃癌D2根治术治疗局部进展期患者是安全、有效的,具有显著的微创优势。这一研究成果引起了学术界的广泛关注。为了在临床实践中正确地认识和科学地应用该研究的成果,本文发布CLASS-01研究共识,以飨读者。内容包括相关定义、手术适应证、器械准备、围手术期处理、手术原则与标准操作流程以及质量控制等关键问题。相关标准操作流程是CLASS-01研究方案撰写时,研究者之间达成的共识,其中的基本原则参考了国际国内权威指南,也充分考虑了我国腹腔镜胃癌外科的实际状况,对我国局部进展期胃癌腹腔镜外科技术的科学发展与合理推广,具有重要的指导意义。 展开更多
关键词 胃肿瘤 局部进展期 D2根治术 远端胃切除术 腹腔镜 规范 共识
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A CLASS OF STRONGLY NONLINEAR SINGULARLY PERTURBED INTERIOR LAYER PROBLEMS 被引量:19
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作者 唐荣荣 《Annals of Differential Equations》 2004年第3期278-282,共5页
In this paper, a class of strongly nonlinear singularly perturbed interior layer problems are considered by the theory of differential inequalities and the corrective theory of interior layer. The existence of solutio... In this paper, a class of strongly nonlinear singularly perturbed interior layer problems are considered by the theory of differential inequalities and the corrective theory of interior layer. The existence of solution is proved and the asymptotic behavior of solution for the boundary value problems are studied. And the satisfying result is obtained. 展开更多
关键词 singular perturbation interior layer problems differential inequality asymptotic behavior
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GLOBAL EXISTENCE FOR A CLASS OF SYSTEMSOF NONLINEAR WAVE EQUATIONS INTHREE SPACE DIMENSIONS
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作者 S.KATAYAMA 《Chinese Annals of Mathematics,Series B》 SCIE CSCD 2004年第4期463-482,共20页
Consider a system of nonlinear wave equations (e)2t-c2i△xui=Fi(u,(e)u,(e)x(e)u) in (0,∞)×(R)3 for i=1,┈,m,where Fi(i=1,┈,m) are smooth functions of degree 2 near the origin of their arguments, and u=(u1,┈,um... Consider a system of nonlinear wave equations (e)2t-c2i△xui=Fi(u,(e)u,(e)x(e)u) in (0,∞)×(R)3 for i=1,┈,m,where Fi(i=1,┈,m) are smooth functions of degree 2 near the origin of their arguments, and u=(u1,┈,um),while (e)u and (e)x(e)u represent the first and (second derivatives of u, respectively. In this paper, the author presents a new class of nonlinearity for which the global existence of small solutions is ensured. For example, global existence of small solutions for((e)2t- c21Δx)u1 = u2((e)tu2) + arbitrary cubic terms,((e)2t - c22Δx)u2=u1((e)tu2) + ((e)tu1)u2 + arbitrary cubic termswill be established, provided that c21 ≠ c22. 展开更多
关键词 波动方程 倍数 处处存在 偏微分方程 非线性
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外泌体传递lncRNA RPL23AP82促进胰腺导管腺癌吉西他滨耐药 被引量:1
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作者 孙运鹏 吴欢欢 +3 位作者 庄磊 陈浩然 徐博 黄侠鸣 《武汉大学学报(医学版)》 2025年第7期850-856,905,共8页
目的:探究外泌体传递lncRNA RPL23AP82促进胰腺导管腺癌(PDAC)吉西他滨(GEM)耐药。方法:(1)构建lncRNA RPL23AP82过表达载体并转染至SW1990细胞中后用GEM进行干扰、分组,对比细胞增殖活性、细胞凋亡。(2)SW1990细胞系转染shRNA‑RPL23AP8... 目的:探究外泌体传递lncRNA RPL23AP82促进胰腺导管腺癌(PDAC)吉西他滨(GEM)耐药。方法:(1)构建lncRNA RPL23AP82过表达载体并转染至SW1990细胞中后用GEM进行干扰、分组,对比细胞增殖活性、细胞凋亡。(2)SW1990细胞系转染shRNA‑RPL23AP82并分组,对比miR‑129、PFTK1 mRNA与PFTK1蛋白表达;SW1990细胞系转染miR‑129 mimics或miR‑129 inhibitor,对比PFTK1 mRNA、PFTK1蛋白表达。(3)SW1990细胞系诱导构建GEM耐受型PDAC细胞(SW1990‑GR)并提取外泌体与亲本细胞孵育,分别根据加入GEM、转染sh‑NC或sh‑RPL23AP82、转染sh‑NC或外泌体抑制剂GW4869,对比lncRNA RPL23AP82表达、GEM敏感性变化、细胞凋亡、细胞增殖活性。结果:(1)对比LV‑NC组,LV‑RPL23AP82组RPL23AP82相对表达量、细胞增殖活性更高且细胞凋亡更低,LV‑NC+GEM组细胞增殖活性更低,LV‑RPL23AP82+GEM组细胞增殖活性低于LV‑RPL23AP82组(均P<0.05)。(2)sh‑RPL23AP82组中RPL23AP82相对表达量低于sh‑NC组,对比sh‑NC组,sh‑RPL23AP82组miR‑129表达升高且PFTK1 mRNA、PFTK1蛋白均下降(均P<0.05);对比NC mimics组,miR‑129 mimics组miR‑129表达升高且miR‑129 inhibitor组miR‑129表达降低,miR‑129 mimics组PFTK1 mRNA和PFTK1蛋白水平降低,而miR‑129 inhibitor组PFTK1 mRNA和PFTK1蛋白水平升高(均P<0.05)。(3)SW1990‑GR组lncRNA RPL23AP82表达、SW1990‑GR组细胞增殖活性升高,对比SW1990‑GR组,SW1990‑GR+GEM组细胞增殖活性下调且细胞凋亡升高,耐药细胞SW1990‑GR与GEM共干扰后细胞凋亡下降(均P<0.05);敲低RPL23AP82后细胞增殖活性下调;GW4869抑制外泌体分泌后GW4869降低,抑制细胞增殖活性;抑制剂GW4869处理细胞增殖活性下降,细胞凋亡上升(均P<0.05)。结论:外泌体上调lncRNA RPL23AP82可降低miR‑129表达并通过刺激PFTK1表达促进吉西他滨耐药。 展开更多
关键词 胰腺导管癌 细胞周期蛋白依赖性激酶14 长链非编码RNA RPL23AP82 微小非编码RNA‑129 细胞增殖 细胞凋亡 吉西他滨 耐药
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