This work aims to study the cultural conditions of pigeon muscle satellite cells and illustrate the nutritional composition of the pigeon-derived cultivated meat.Methods for cell disaggregation and purification were o...This work aims to study the cultural conditions of pigeon muscle satellite cells and illustrate the nutritional composition of the pigeon-derived cultivated meat.Methods for cell disaggregation and purification were optimized,high-purity pigeon skeletal muscle satellite cells were successfully isolated.Meanwhile,the cultivation conditions for cell proliferation and multi-directional differentiation were optimized.Results demonstrated that pigeon muscle satellite cells exhibited rapid proliferation and spontaneous differentiation at 41℃,McCoy’s 5a was significantly superior to other cultural mediums for cell growth,DMEM with high glucose promote myogenic differentiation of pigeon skeletal muscle satellite cells and chicken serum can play important role in inducing the pigeon muscle satellite cells into adipocytes.additionally,increasing the concentration of serum in medium has a positive promoting effect on the respective effects.Nutritional composition and biomarkers of the pigeon-derived cultivated meat was first illustrated in this work by proteomics and lipidomics,respectively.Proteomics revealed that 140 proteins were identified as major aberrant proteins and 648 proteins were identified from the after myogenic differentiation samples,including 12 collagens,15 myosins,7 actins,etc.Lipidomics revealed that 73 lipids were identified as major aberrant lipids and 297 lipids were identified from the after lipidogenic differentiation,including 102 triglycerides,52 diacylglycerols,59 phosphatidyl cholines,etc.展开更多
基金supported by the National Key Research and Development Program of China (2021YFC2101400)the Beijing Science and Technology Program (Z221100006422011)
文摘This work aims to study the cultural conditions of pigeon muscle satellite cells and illustrate the nutritional composition of the pigeon-derived cultivated meat.Methods for cell disaggregation and purification were optimized,high-purity pigeon skeletal muscle satellite cells were successfully isolated.Meanwhile,the cultivation conditions for cell proliferation and multi-directional differentiation were optimized.Results demonstrated that pigeon muscle satellite cells exhibited rapid proliferation and spontaneous differentiation at 41℃,McCoy’s 5a was significantly superior to other cultural mediums for cell growth,DMEM with high glucose promote myogenic differentiation of pigeon skeletal muscle satellite cells and chicken serum can play important role in inducing the pigeon muscle satellite cells into adipocytes.additionally,increasing the concentration of serum in medium has a positive promoting effect on the respective effects.Nutritional composition and biomarkers of the pigeon-derived cultivated meat was first illustrated in this work by proteomics and lipidomics,respectively.Proteomics revealed that 140 proteins were identified as major aberrant proteins and 648 proteins were identified from the after myogenic differentiation samples,including 12 collagens,15 myosins,7 actins,etc.Lipidomics revealed that 73 lipids were identified as major aberrant lipids and 297 lipids were identified from the after lipidogenic differentiation,including 102 triglycerides,52 diacylglycerols,59 phosphatidyl cholines,etc.
