Soybean is one of the major oil seed crops,which is usually intercropped with other crops to increase soybean production area and yield.However,soybean is highly sensitive to shading.It is unclear if soybean morpholog...Soybean is one of the major oil seed crops,which is usually intercropped with other crops to increase soybean production area and yield.However,soybean is highly sensitive to shading.It is unclear if soybean morphology responds to shading（i.e.,shade tolerance or avoidance）and which features may be suitable as screening materials in relay strip intercropping.Therefore,in this study,various agronomic characteristics of different soybean genotypes were analyzed under relay intercropping conditions.The soybean materials used in this study exhibited genetic diversity,and the coefficient of variations of the agronomic parameters ranged from 13.84 to 72.08%during the shade period and from 6.44 to 52.49%during the maturity period.The ratios of shading to full irradiance in stem mass fraction（SMF）were almost greater than 1,whereas opposite results were found in the leaves.Compared with full irradiance,the average stem length（SL）,leaf area ratio（LAR）and specific leaf area（SLA）for the two years（2013 and 2014）increased by 0.78,0.47 and 0.65 under shady conditions,respectively.However,the stem diameter（SD）,total biomass（TB）,leaf area（LA）,number of nodes（NN）on the main stem,and number of branches（BN）all decreased.During the shady period,the SL and SMF exhibited a significant negative correlation with yield,and the SD exhibited a significant positive correlation with yield.The correlation between the soybean yield and agronomic parameters during the mature period,except for SL,the first pod height（FPH）,100-seed weight（100-SW）,and reproductive growth period（RGP）,were significant（P〈0.01）,especially for seed weight per branch（SWB）,pods per plant（PP）,BN,and vegetative growth period（VGP）.These results provide an insight into screening the shade tolerance of soybean varieties and can be useful in targeted breeding programs of relay intercropped soybeans.展开更多
Persistent tapetal cell1(PTC1) plays a curial role in pollen development, and is thought to function as a transcriptional activator in rice. However, the molecular mechanism of PTC1 in regulating pollen development an...Persistent tapetal cell1(PTC1) plays a curial role in pollen development, and is thought to function as a transcriptional activator in rice. However, the molecular mechanism of PTC1 in regulating pollen development and its cis-elements are not well understood. We identified a novel weak male sterility mutant(ms92) which exhibited expanded tapetum and shrink pollen grains. Map-based cloning and allelic analysis suggested that the male sterility of ms92 was caused by a DNA fragment substitution in the promoter of PTC1. The decreased expression of MS92/PTC1 in ms92 and cis-element analysis indicated that the substituted sequence contained several potential binding cis-element of negative feedback. MS92/PTC1 was specifically expressed in tapetum and microspores at the young microspore stage, and its protein was localized in nucleus. We further found that MS92/PTC1 functions as a transcription activator by recognizing H3K4me3. Transcriptomic analysis revealed that a number of genes involved in tapetum degeneration and pollen wall formation were down-regulated in ms92, which are the potential targets of MS92/PTC1. The substitution fragment in MS92/PTC1 promoter was essential for pollen development, and we provided a novel mutant for further identifying the cis-elements in promoter and the molecular network of MS92/PTC1.展开更多
The research analyzed the theoretical basis of the sustainable development of the Chongqing lava bean in terms of the current market economy situation. In regard to practice, several strategies will be suggested for t...The research analyzed the theoretical basis of the sustainable development of the Chongqing lava bean in terms of the current market economy situation. In regard to practice, several strategies will be suggested for the Chongqing fava bean industry to guide the development of Chongqing＇s efficient fava bean agriculture.展开更多
基金supported by the National Natural Science Foundation of China (31571615)the National Key Research and Development Program of China (2016YFD0300602, 2016YFD0300209)+1 种基金the Major Project of Education Department in Sichuan, China (16ZA0041)the earmarked fund for China Agriculture Research System, China (CARS-04-PS19)
文摘Soybean is one of the major oil seed crops,which is usually intercropped with other crops to increase soybean production area and yield.However,soybean is highly sensitive to shading.It is unclear if soybean morphology responds to shading（i.e.,shade tolerance or avoidance）and which features may be suitable as screening materials in relay strip intercropping.Therefore,in this study,various agronomic characteristics of different soybean genotypes were analyzed under relay intercropping conditions.The soybean materials used in this study exhibited genetic diversity,and the coefficient of variations of the agronomic parameters ranged from 13.84 to 72.08%during the shade period and from 6.44 to 52.49%during the maturity period.The ratios of shading to full irradiance in stem mass fraction（SMF）were almost greater than 1,whereas opposite results were found in the leaves.Compared with full irradiance,the average stem length（SL）,leaf area ratio（LAR）and specific leaf area（SLA）for the two years（2013 and 2014）increased by 0.78,0.47 and 0.65 under shady conditions,respectively.However,the stem diameter（SD）,total biomass（TB）,leaf area（LA）,number of nodes（NN）on the main stem,and number of branches（BN）all decreased.During the shady period,the SL and SMF exhibited a significant negative correlation with yield,and the SD exhibited a significant positive correlation with yield.The correlation between the soybean yield and agronomic parameters during the mature period,except for SL,the first pod height（FPH）,100-seed weight（100-SW）,and reproductive growth period（RGP）,were significant（P〈0.01）,especially for seed weight per branch（SWB）,pods per plant（PP）,BN,and vegetative growth period（VGP）.These results provide an insight into screening the shade tolerance of soybean varieties and can be useful in targeted breeding programs of relay intercropped soybeans.
基金supported by the National Natural Science Foundation of China(Grant No.31301054)。
文摘Persistent tapetal cell1(PTC1) plays a curial role in pollen development, and is thought to function as a transcriptional activator in rice. However, the molecular mechanism of PTC1 in regulating pollen development and its cis-elements are not well understood. We identified a novel weak male sterility mutant(ms92) which exhibited expanded tapetum and shrink pollen grains. Map-based cloning and allelic analysis suggested that the male sterility of ms92 was caused by a DNA fragment substitution in the promoter of PTC1. The decreased expression of MS92/PTC1 in ms92 and cis-element analysis indicated that the substituted sequence contained several potential binding cis-element of negative feedback. MS92/PTC1 was specifically expressed in tapetum and microspores at the young microspore stage, and its protein was localized in nucleus. We further found that MS92/PTC1 functions as a transcription activator by recognizing H3K4me3. Transcriptomic analysis revealed that a number of genes involved in tapetum degeneration and pollen wall formation were down-regulated in ms92, which are the potential targets of MS92/PTC1. The substitution fragment in MS92/PTC1 promoter was essential for pollen development, and we provided a novel mutant for further identifying the cis-elements in promoter and the molecular network of MS92/PTC1.
基金Supported by csct2012jj A80042cstc2013yykfc80002+5 种基金Ycstc,2013ac1001Ycstc,2014ac1010Ycstc,2014nc1004201303129CARS-09Construction of Mutant Bank of "Tongcanxian No.8" Induced by EMS
文摘The research analyzed the theoretical basis of the sustainable development of the Chongqing lava bean in terms of the current market economy situation. In regard to practice, several strategies will be suggested for the Chongqing fava bean industry to guide the development of Chongqing＇s efficient fava bean agriculture.
