[Objective]The paper was to establish a rapid identification method of Bactrocera cilifera(Hendel)with species-specific primers(SS-COI).[Method]Using B.cilifera(Hendel)as the positive control,and 19 species of fruit f...[Objective]The paper was to establish a rapid identification method of Bactrocera cilifera(Hendel)with species-specific primers(SS-COI).[Method]Using B.cilifera(Hendel)as the positive control,and 19 species of fruit flies such as B.diaphora(Coquillett)and B.dorsalis(Hendel)as the negative controls,a pair of species-specific primers,YF290 and YR511,were designed and screened for accurate identification of B.cilifera,based on mitochondrial DNA COI sequence.[Result]The PCR products were amplified and detected by electrophoresis.Only a clear and single band was observed at about 222 bp in the positive control,while no bands were found in the other negative controls.[Conclusion]The established rapid identification method with species-specific primers(SS-COI)is of great practical significance for rapid identification of fruit flies intercepted from import and export fruits and vegetables at ports,and for rapid clearance and early warning of import fruits and vegetables at ports.展开更多
One new species,Bactrocera(Bactrocera)latizona Huang,Yu&Bai sp.nov.,from Mengla,Yunnan Province,China is described.Illustrations of the new species and a key to common species of the subgenus of Bactrocera from Yu...One new species,Bactrocera(Bactrocera)latizona Huang,Yu&Bai sp.nov.,from Mengla,Yunnan Province,China is described.Illustrations of the new species and a key to common species of the subgenus of Bactrocera from Yunnan are given.展开更多
[Objectives]To analyze the evolutionary rates of mitochondrial protein-coding genes across five closely related species of fruit flies,thereby providing a foundation for the molecular identification of these quarantin...[Objectives]To analyze the evolutionary rates of mitochondrial protein-coding genes across five closely related species of fruit flies,thereby providing a foundation for the molecular identification of these quarantine pests.[Methods]The newly identified species Bactrocera latizona,along with its closely related species within the same subgenus,namely B.atrifemur,B.rubigina,B.thailandica,and B.tuberculata,were selected as the subjects of this study.Utilizing the complete mitochondrial genome sequences of these five fruit fly species,the Ka/Ks ratios of 13 protein-coding genes were calculated to assess their selective pressures and degrees of conservation.[Results]The mitochondrial genome lengths of the five fruit fly species ranged from 15603 to 15972 bp.The Ka/Ks ratios of the ND4L and ND4 genes for all species were generally elevated(values of the ND4L gene all exceeding 2),suggesting accelerated evolutionary rates.In contrast,the COX1 gene exhibited the lowest Ka/Ks ratio,indicating it is the most conserved gene among those analyzed.The majority of genes displayed Ka/Ks ratios below 1,implying they are under purifying selection.[Conclusions]Among the mitochondrial genes of five fruit fly species,COX1 is the most conserved,whereas ND4L exhibits the highest rate of evolution.These findings offer theoretical support for the development of molecular markers and the species identification of fruit flies.展开更多
基金Supported by Natural Science Foundation of Fujian Province (2011J01066, 2012JO1061)。
文摘[Objective]The paper was to establish a rapid identification method of Bactrocera cilifera(Hendel)with species-specific primers(SS-COI).[Method]Using B.cilifera(Hendel)as the positive control,and 19 species of fruit flies such as B.diaphora(Coquillett)and B.dorsalis(Hendel)as the negative controls,a pair of species-specific primers,YF290 and YR511,were designed and screened for accurate identification of B.cilifera,based on mitochondrial DNA COI sequence.[Result]The PCR products were amplified and detected by electrophoresis.Only a clear and single band was observed at about 222 bp in the positive control,while no bands were found in the other negative controls.[Conclusion]The established rapid identification method with species-specific primers(SS-COI)is of great practical significance for rapid identification of fruit flies intercepted from import and export fruits and vegetables at ports,and for rapid clearance and early warning of import fruits and vegetables at ports.
文摘One new species,Bactrocera(Bactrocera)latizona Huang,Yu&Bai sp.nov.,from Mengla,Yunnan Province,China is described.Illustrations of the new species and a key to common species of the subgenus of Bactrocera from Yunnan are given.
文摘[Objectives]To analyze the evolutionary rates of mitochondrial protein-coding genes across five closely related species of fruit flies,thereby providing a foundation for the molecular identification of these quarantine pests.[Methods]The newly identified species Bactrocera latizona,along with its closely related species within the same subgenus,namely B.atrifemur,B.rubigina,B.thailandica,and B.tuberculata,were selected as the subjects of this study.Utilizing the complete mitochondrial genome sequences of these five fruit fly species,the Ka/Ks ratios of 13 protein-coding genes were calculated to assess their selective pressures and degrees of conservation.[Results]The mitochondrial genome lengths of the five fruit fly species ranged from 15603 to 15972 bp.The Ka/Ks ratios of the ND4L and ND4 genes for all species were generally elevated(values of the ND4L gene all exceeding 2),suggesting accelerated evolutionary rates.In contrast,the COX1 gene exhibited the lowest Ka/Ks ratio,indicating it is the most conserved gene among those analyzed.The majority of genes displayed Ka/Ks ratios below 1,implying they are under purifying selection.[Conclusions]Among the mitochondrial genes of five fruit fly species,COX1 is the most conserved,whereas ND4L exhibits the highest rate of evolution.These findings offer theoretical support for the development of molecular markers and the species identification of fruit flies.
