Objective Little is known about the association between whole-blood nicotinamide adenine dinucleotide(NAD^(+))levels and nabothian cysts.This study aimed to assess the association between NAD^(+)levels and nabothian c...Objective Little is known about the association between whole-blood nicotinamide adenine dinucleotide(NAD^(+))levels and nabothian cysts.This study aimed to assess the association between NAD^(+)levels and nabothian cysts in healthy Chinese women.Methods Multivariate logistic regression analysis was performed to analyze the association between NAD^(+)levels and nabothian cysts.Results The mean age was 43.0±11.5 years,and the mean level of NAD^(+)was 31.3±5.3μmol/L.Nabothian cysts occurred in 184(27.7%)participants,with single and multiple cysts in 100(15.0%)and84(12.6%)participants,respectively.The total nabothian cyst prevalence gradually decreased from37.4%to 21.6%from Q1 to Q4 of NAD^(+)and the prevalence of single and multiple nabothian cysts also decreased across the NAD^(+)quartiles.As compared with the highest NAD^(+)quartile(≥34.4μmol/L),the adjusted odds ratios with 95%confidence interval of the NAD^(+)Q1 was 1.89(1.14–3.14)for total nabothian cysts.The risk of total and single nabothian cysts linearly decreased with increasing NAD^(+)levels,while the risk of multiple nabothian cysts decreased more rapidly at NAD^(+)levels of 28.0 to35.0μmol/L.Conclusion:Low NAD^(+)levels were associated with an increased risk of total and multiple nabothian cysts.展开更多
Objective To describe the temporal trends and spatial patterns of birth defects occurring in Wuxi, a developed region of China. Methods Wavelet analysis was used to decompose the temporal trends of birth defect preval...Objective To describe the temporal trends and spatial patterns of birth defects occurring in Wuxi, a developed region of China. Methods Wavelet analysis was used to decompose the temporal trends of birth defect prevalence based on the birth defect rates over the past 16 years. Birth defect cases with detailed personal and family information were geo-coded and the relative risk in each village was calculated. General G statistic was used to test the spatial property with different scales. Results Wavelet analysis showed an increasing temporal trend of birth defects in this region. Clustering analysis revealed that changes continued in the spatial patterns with different scales. Conclusion Wuxi is confronted with severe challenges to reduce birth defect prevalence. The risk factors are stable and show no change with spatial scale but an increasing temporal trend. Interventions should be focused on villages with a higher prevalence of birth defects.展开更多
Male infertility is a global issue caused by poor sperm quality,particularly motility.Enhancement of the sperm quality may improve the fertilization rate in assisted reproductive technology(ART)treatment.Scriptaid,wit...Male infertility is a global issue caused by poor sperm quality,particularly motility.Enhancement of the sperm quality may improve the fertilization rate in assisted reproductive technology(ART)treatment.Scriptaid,with a novel human sperm motility-stimulating activity,has been investigated as a prospective agent for improving sperm quality and fertilization rate in ART.We evaluated the effects of Scriptaid on asthenozoospermic(AZS)semen,including its impact on motility stimulation and protective effects on cryopreservation and duration of motility,by computer-aided sperm analysis(CASA).Sperm quality improvement by Scriptaid was characterized by increased hyaluronan-binding activity,tyrosine phosphorylation,adenosine triphosphate(ATP)concentration,mitochondrial membrane potential,and an ameliorated AZS fertilization rate in clinical intracytoplasmic sperm injection(ICSI)experiments.Furthermore,our identification of active Scriptaid analogs and different metabolites induced by Scriptaid in spermatozoa lays a solid foundation for the future biomechanical exploration of sperm function.In summary,Scriptaid is a potential candidate for the treatment of male infertility in vitro as it improves sperm quality,prolongs sperm viability,and increases the fertilization rate.展开更多
Recent studies have explored the spatial transcriptomics patterns of Alzheimer's disease(AD)brain by spatial sequencing in mouse models,enabling the identification of unique genome-wide transcriptomic features ass...Recent studies have explored the spatial transcriptomics patterns of Alzheimer's disease(AD)brain by spatial sequencing in mouse models,enabling the identification of unique genome-wide transcriptomic features associated with different spatial regions and pathological status.However,the dynamics of gene interactions that occur during amyloid-βaccumulation remain largely unknown.In this study,we performed analyses on ligand-receptor communication,transcription factor regulatory network,and spot-specific network to reveal the dependence and the dynamics of gene associations/interactions on spatial regions and pathological status with mouse and human brains.We first used a spatial transcriptomics dataset of the AppNL-G-F knock-in AD and wild-type mouse model.We revealed 17 ligand-receptor pairs with opposite tendencies throughout the amyloid-βaccumulation process and showed the specific ligand-receptor interactions across the hippocampus layers at different extents of pathological changes.We then identified nerve function related transcription factors in the hippocampus and entorhinal cortex,as well as genes with different transcriptomic association degrees in AD versus wild-type mice.Finally,another independent spatial transcriptomics dataset from different AD mouse models and human single-nuclei RNA-seq data/AlzData database were used for validation.This is the first study to identify various gene associations throughout amyloid-βaccumulation based on spatial transcriptomics,establishing the foundations to reveal advanced and in-depth AD etiology from a novel perspective based on the comprehensive analyses of gene interactions that are spatio-temporal dependent.展开更多
Objective:To investigate the impact of endometriosis(EMS)on granulosa cell function and elucidate the molecular mechanisms involved.Methods:RNA sequencing,differential expression analysis,Gene Ontology(GO)and Kyoto En...Objective:To investigate the impact of endometriosis(EMS)on granulosa cell function and elucidate the molecular mechanisms involved.Methods:RNA sequencing,differential expression analysis,Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis,gene set enrichment analysis,protein–protein interaction analysis,and RT-qPCR were employed to assess the effects of EMS on granulosa cell function.Results:The results revealed significant differences in gene expression between the EMS and control groups,including genes related to immune regulatory functions and ferroptosis.Hub gene modules and hub genes were identified,including those related to cell cycle and immune and inflammatory pathways.RT-qPCR revealed significant upregulation of the hub genesCCL3 andIL1B in granulosa cells of patients with EMS.Conclusion:The results of RNA sequencing demonstrated that EMS induces significant transcriptional alterations in granulosa cells of affected patients.These findings provide important insights into the diagnosis and treatment of EMS and highlight the importance of further investigation ofCCL3 andIL1B as potential biomarkers for EMS.展开更多
基金supported by grants from the NSFC-Regional Innovation and Development Joint Fund(No.U22A20364)the National Key R&D Program of China(No.2021YFC2500500)the National Natural Science Foundation of China(No.81973112,No.92049302)。
文摘Objective Little is known about the association between whole-blood nicotinamide adenine dinucleotide(NAD^(+))levels and nabothian cysts.This study aimed to assess the association between NAD^(+)levels and nabothian cysts in healthy Chinese women.Methods Multivariate logistic regression analysis was performed to analyze the association between NAD^(+)levels and nabothian cysts.Results The mean age was 43.0±11.5 years,and the mean level of NAD^(+)was 31.3±5.3μmol/L.Nabothian cysts occurred in 184(27.7%)participants,with single and multiple cysts in 100(15.0%)and84(12.6%)participants,respectively.The total nabothian cyst prevalence gradually decreased from37.4%to 21.6%from Q1 to Q4 of NAD^(+)and the prevalence of single and multiple nabothian cysts also decreased across the NAD^(+)quartiles.As compared with the highest NAD^(+)quartile(≥34.4μmol/L),the adjusted odds ratios with 95%confidence interval of the NAD^(+)Q1 was 1.89(1.14–3.14)for total nabothian cysts.The risk of total and single nabothian cysts linearly decreased with increasing NAD^(+)levels,while the risk of multiple nabothian cysts decreased more rapidly at NAD^(+)levels of 28.0 to35.0μmol/L.Conclusion:Low NAD^(+)levels were associated with an increased risk of total and multiple nabothian cysts.
基金the National "973" Project on Population and Health (No. 2007CB5119001)the National Yang Zi Scholar Program,211 and 985 Projects of Peking University (No. 20020903)
文摘Objective To describe the temporal trends and spatial patterns of birth defects occurring in Wuxi, a developed region of China. Methods Wavelet analysis was used to decompose the temporal trends of birth defect prevalence based on the birth defect rates over the past 16 years. Birth defect cases with detailed personal and family information were geo-coded and the relative risk in each village was calculated. General G statistic was used to test the spatial property with different scales. Results Wavelet analysis showed an increasing temporal trend of birth defects in this region. Clustering analysis revealed that changes continued in the spatial patterns with different scales. Conclusion Wuxi is confronted with severe challenges to reduce birth defect prevalence. The risk factors are stable and show no change with spatial scale but an increasing temporal trend. Interventions should be focused on villages with a higher prevalence of birth defects.
文摘Male infertility is a global issue caused by poor sperm quality,particularly motility.Enhancement of the sperm quality may improve the fertilization rate in assisted reproductive technology(ART)treatment.Scriptaid,with a novel human sperm motility-stimulating activity,has been investigated as a prospective agent for improving sperm quality and fertilization rate in ART.We evaluated the effects of Scriptaid on asthenozoospermic(AZS)semen,including its impact on motility stimulation and protective effects on cryopreservation and duration of motility,by computer-aided sperm analysis(CASA).Sperm quality improvement by Scriptaid was characterized by increased hyaluronan-binding activity,tyrosine phosphorylation,adenosine triphosphate(ATP)concentration,mitochondrial membrane potential,and an ameliorated AZS fertilization rate in clinical intracytoplasmic sperm injection(ICSI)experiments.Furthermore,our identification of active Scriptaid analogs and different metabolites induced by Scriptaid in spermatozoa lays a solid foundation for the future biomechanical exploration of sperm function.In summary,Scriptaid is a potential candidate for the treatment of male infertility in vitro as it improves sperm quality,prolongs sperm viability,and increases the fertilization rate.
基金supported by grants from the National Natural Science Foundation of China (No.81902277)the National Key R&D Program of China (No.2017YFC1001100).
文摘Recent studies have explored the spatial transcriptomics patterns of Alzheimer's disease(AD)brain by spatial sequencing in mouse models,enabling the identification of unique genome-wide transcriptomic features associated with different spatial regions and pathological status.However,the dynamics of gene interactions that occur during amyloid-βaccumulation remain largely unknown.In this study,we performed analyses on ligand-receptor communication,transcription factor regulatory network,and spot-specific network to reveal the dependence and the dynamics of gene associations/interactions on spatial regions and pathological status with mouse and human brains.We first used a spatial transcriptomics dataset of the AppNL-G-F knock-in AD and wild-type mouse model.We revealed 17 ligand-receptor pairs with opposite tendencies throughout the amyloid-βaccumulation process and showed the specific ligand-receptor interactions across the hippocampus layers at different extents of pathological changes.We then identified nerve function related transcription factors in the hippocampus and entorhinal cortex,as well as genes with different transcriptomic association degrees in AD versus wild-type mice.Finally,another independent spatial transcriptomics dataset from different AD mouse models and human single-nuclei RNA-seq data/AlzData database were used for validation.This is the first study to identify various gene associations throughout amyloid-βaccumulation based on spatial transcriptomics,establishing the foundations to reveal advanced and in-depth AD etiology from a novel perspective based on the comprehensive analyses of gene interactions that are spatio-temporal dependent.
基金the National Natural Science Foundation of China(82071598)。
文摘Objective:To investigate the impact of endometriosis(EMS)on granulosa cell function and elucidate the molecular mechanisms involved.Methods:RNA sequencing,differential expression analysis,Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis,gene set enrichment analysis,protein–protein interaction analysis,and RT-qPCR were employed to assess the effects of EMS on granulosa cell function.Results:The results revealed significant differences in gene expression between the EMS and control groups,including genes related to immune regulatory functions and ferroptosis.Hub gene modules and hub genes were identified,including those related to cell cycle and immune and inflammatory pathways.RT-qPCR revealed significant upregulation of the hub genesCCL3 andIL1B in granulosa cells of patients with EMS.Conclusion:The results of RNA sequencing demonstrated that EMS induces significant transcriptional alterations in granulosa cells of affected patients.These findings provide important insights into the diagnosis and treatment of EMS and highlight the importance of further investigation ofCCL3 andIL1B as potential biomarkers for EMS.
