AIM:To explore the repeatability,reproducibility,and agreement in the measurement of the choroidal vascularity index(CVI)for different swept-source optical coherence tomography(OCT)devices and between OCT and OCT angi...AIM:To explore the repeatability,reproducibility,and agreement in the measurement of the choroidal vascularity index(CVI)for different swept-source optical coherence tomography(OCT)devices and between OCT and OCT angiography(OCTA)images.METHODS:Two swept-source OCT imaging systems,VG200I and Topcon DRI OCT Triton,were used to capture OCT and OCTA images in triplicate.The first and third images were taken by one operator,and the second image was taken by another operator.The built-in software was used to calculate the CVI from the OCTA images(CVI-OCTA),and a custom-designed algorithm was used to calculate the CVI from the OCT images(CVI-OCT).Repeatability and reproducibility were assessed with the intraclass correlation coefficient(ICC),and agreement between devices and between OCT and OCTA were evaluated with Bland-Altman analysis.RESULTS:Sixty-eight eyes from 35 adults(17 females)were included in the analysis.The average age of the participants was 23.6±2.3y,with an average spherical equivalent refraction of-3.08±2.47 D and an average AL of 25.21±1.20 mm.Both OCT devices demonstrated high repeatability and reproducibility in measuring the CVI-OCTA(all ICCs>0.894 across five choroidal regions)and CVI-OCT(all ICCs>0.838).Furthermore,the between-device agreement in measuring the CVI-OCT was good[mean difference(MD)ranging from-2.32%to-3.07%],but that in measuring the CVI-OCTA was poor(MD,1.48%to-7.43%).Additionally,the between-imaging agreement(CVI-OCTA versus CVI-OCT)was poor for both devices(Triton,MD,6.05%to 12.68%;VG200I,MD,6.67%to 12.09%).CONCLUSION:Both OCT devices and the two analytical methods demonstrate good stability.The inter-device consistency of CVI-OCT is good,while the inter-device consistency of CVI-OCTA and the consistency between the two analytical methods in the same device are both poor.展开更多
AIM:To evaluate the differences and consistency of vault measurements obtained by Scheimpflug tomography(Pentacam),anterior segment optical coherence tomography(AS-OCT,CASIA II),and ultrasound biomicroscopy(UBM)follow...AIM:To evaluate the differences and consistency of vault measurements obtained by Scheimpflug tomography(Pentacam),anterior segment optical coherence tomography(AS-OCT,CASIA II),and ultrasound biomicroscopy(UBM)following implantable collamer lens(ICL)V4c implantation.METHODS:Vault measurements were acquired using three modalities:Pentacam,CASIA II AS-OCT,and UBM.Repeated-measures analysis of variance was used to compare the vault values obtained by the three devices.The correlation and consistency of measurements among the three instruments were assessed using the Pearson correlation coefficient,intraclass correlation coefficient(ICC),and Bland-Altman plots.RESULTS:This retrospective study enrolled 210 myopic eyes of 210 patients(158 women and 52 men)who underwent ICL implantation:108 eyes had a myopic ICL V4c implanted,and 102 eyes had a toric ICL V4c implanted.The mean vault values measured by Pentacam,CASIA II,and UBM were 452.64±204.20μm,538.57±203.54μm,and 560.95±227.54μm,respectively,with statistically significant differences among the three groups(P<0.05).Pearson correlation analysis showed strong positive correlations between vault values measured by different instruments(all P<0.001).ICC results indicated good consistency among the three measurement modalities(all P<0.001).Stratified analysis revealed that when the vault value was≤250μm,the correlation and consistency of measurements across the three instruments were lower than those in the medium and high vault subgroups.CONCLUSION:Vault values measured by Pentacam are lower than those obtained by CASIA II and UBM,with UBM yielding the highest mean vault values.Measurements from the three instruments are not interchangeable but can serve as mutual references due to their significant correlation and good overall consistency.Pentacam and CASIA II demonstrate the highest consistency in vault measurement.Notably,when the vault value is≤250μm,the consistency between Pentacam and the other two instruments decreases significantly.展开更多
Self-healing hydrogels utilize inherent intermolecular forces to autonomously heal physical damage resulting from excessive strain,pressure,or tearing.Applying these materials in soft robotics and tissue engineering c...Self-healing hydrogels utilize inherent intermolecular forces to autonomously heal physical damage resulting from excessive strain,pressure,or tearing.Applying these materials in soft robotics and tissue engineering could be beneficial.On the other hand,their efficacy in stretchable and mechanically resistant circuits is hindered by their limited electrical conductivity.展开更多
AIM:To analyze the environmental impact of patient travel for cataract surgery at a German ophthalmology center.METHODS:All cataract surgeries performed between October 23 and October 27,2023,were analyzed,and all pat...AIM:To analyze the environmental impact of patient travel for cataract surgery at a German ophthalmology center.METHODS:All cataract surgeries performed between October 23 and October 27,2023,were analyzed,and all patient records were reviewed for follow-up visits.All travel distances were calculated,and the associated emissions were quantified.Additionally,patients’utilization of geographically closer branch practices for follow-up care was evaluated,along with the corresponding effects on travel-related emissions.RESULTS:A total of 69 patients underwent unilateral cataract surgery.The average one-way travel distance was 40.1 km(24.9 mi;SD=23.6 km).Corresponding emissions were 1284.8 kg of greenhouse gas(GHG),2.477 kg of nitrogen oxides,and 0.101 kg of particulates.All patients attended at least two follow-up visits.Conducting followup visits at branch practices reduced travel distance by 49.1%.The associated GHG emissions from all travel were 1984.3 kg.Emissions from follow-up visits were 54.4%higher than those from the surgery itself.Total GHG emissions amounted to 3269.1 kg,with an average of 47.4 kg of GHG per patient for all travel associated with cataract surgery.CONCLUSION:A dense network of branch practices contributes to reducing the carbon footprint of cataract surgery-related patient travel;however,the development of digital health approaches for follow-up care is necessary to further optimize the environmental sustainability of cataract surgery.展开更多
In the fight against bacterial infections,it is critical to effectively disrupt biofilms.However,disruption of biofilms becomes exceptionally difficult due to the low permeability of therapeutic agents.Herein,we prese...In the fight against bacterial infections,it is critical to effectively disrupt biofilms.However,disruption of biofilms becomes exceptionally difficult due to the low permeability of therapeutic agents.Herein,we present a self-propelled nanovesicle(PCL-PLG@CHX)strategy for eliminating biofilms and further expediting the healing of wounds.PCL-PLG@CHX is synthesized by assembling vesicles from amphiphilic polymers,which incorporate both poly-ε-caprolactone and guanidinated-poly-ε-lysine(PCL-PLG)and are infused with chlorhexidine(CHX).Upon application to sites of bacterial infection,PCL-PLG@CHX,abundant in guanidinium structures,effectively accumulates on the negatively charged surface of biofilms.It interacts with reactive oxygen species(ROS)within the biofilm,leading to nitric oxide(NO)production.The generated NO cannot only propel the nanovesicle to penetrate deeper into the biofilm,but also act as a signaling molecule to disperse the biofilm,working in conjunction with the subsequent release of CHX for an enhanced antibacterial impact.Following the eradication of bacteria,the residual guanidine component continues to produce small quantities of NO,facilitating angiogenesis and epithelial growth,thereby accelerating the healing of wounds.Together,our study shows that PCL-PLG@CHX utilizes the potential of guanidine moieties to efficiently break down biofilms and support tissue restoration,tackling the pivotal challenge of biofilm-related diseases.展开更多
AIM:To investigate the ocular surface parameters in patients with moderate to high myopia.METHODS:This prospective study was conducted in May 2023,enrolling patients with moderate to high myopia(spherical equivalent r...AIM:To investigate the ocular surface parameters in patients with moderate to high myopia.METHODS:This prospective study was conducted in May 2023,enrolling patients with moderate to high myopia(spherical equivalent refraction≤-3.0 D).After completing the Ocular Surface Disease Index(OSDI)questionnaire,refractive parameters and non-invasive tear film parameters were measured,followed by the Schirmer I test and fluorescein sodium staining.The diagnosis of dry eye disease was based on OSDI score,non-invasive tear breakup time(NIBUT),Schirmer I test,and fluorescein sodium staining results,according to the 2020 Chinese Expert Consensus on Dry Eye.The Mann-Whitney U test was used to compare ocular surface parameters between moderate and high myopia,as well as between patients with and without dry eye.Pearson correlation analysis was employed to assess the relationship between the lipid/muco-aqueous layers and tear film parameters.A general linear mixed model(GLMM)was used to analyze the impact of refractive parameters on ocular surface parameters after adjusting for age and sex.RESULTS:A total of 35 eyes with moderate to high myopia(12 males;mean age,30.30±5.45y)were included in the study.Among them,26 eyes were classified as normal and 9 as dry eye.Of the 9 dry eye cases,7 were observed in the high myopia group(n=18)and 2 in the moderate myopia group(n=17).Among the enrolled patients,those with high myopia demonstrated significantly higher OSDI scores than those with moderate myopia(P=0.0417).Patients with dry eye exhibited significantly shorter interblink intervals(P=0.0081)and higher OSDI scores(P=0.0001)than those without dry eye.Pearson correlation analysis revealed a significant positive correlation between lipid layer thickness(LLT)and tear meniscus height(r=0.395,P=0.023),and a significant negative correlation between the muco-aqueous layer thickness change rate(MALTR)and OSDI score(r=-0.466,P=0.016).After adjusting for age and sex using the GLMM,spherical refraction(SPH,β=-1.802,P=0.048)and axial length(AL,β=2.784,P=0.048)significantly impacted OSDI score.Corneal front astigmatism significantly influenced Schirmer I test results(β=8.377,P=0.024).The difference between central corneal thickness and the thinnest corneal thickness significantly affected LLT(β=-2.294,P=0.026).White-to-white diameter significantly impacted MALTR(β=-81.758,P=0.037).CONCLUSION:In moderate to high myopia,higher SPH and AL correlate with increased dry eye symptoms,which are associated with muco-aqueous and lipid layer alterations.Corneal regularity and diameter also affect tear film dynamics.展开更多
Tumor heterogeneity and diversity significantly undermine the effectiveness of monotherapy.Collaborative therapy emerges as a promising approach to mitigate tumor recurrence resulting from monotherapy.Combining chemod...Tumor heterogeneity and diversity significantly undermine the effectiveness of monotherapy.Collaborative therapy emerges as a promising approach to mitigate tumor recurrence resulting from monotherapy.Combining chemodynamic therapy(CDT)with photothermal therapy(PTT)offers a compelling solution for eradicating residual tumor cells post-PTT.In this study,we harness the Fenton-like response facilitated by glucose oxidase(GOD)and the mild hyperthermia induced by polyethyleneimine(PEI)functionalized nitrogen-containing graphene oxide to enhance tumor therapy through a metal-free bionic nanozyme.GOD catalyzes a substantial amount of hydrogen peroxide,and,with the carrier's involvement,triggers a Fenton-like reaction,yielding a wealth of hydroxyl radicals.These hydroxyl radicals effectively target tumor cells following photothermal action,bolstering CDT and culminating in a bidirectional amplification treatment that effectively prevents tumor recurrence and metastasis.This research amalgamates the physical and chemical attributes of nanomaterials with the unique characteristics of the tumor microenvironment,presenting a compelling and efficacious alternative for tumor treatment.展开更多
Polydopamine(PD)coating,one of the simplest and most versatile surface functionalization method faces challenges in terms of stability and reactivity.In this study,we propose an in situ dynamic reassembly approach to ...Polydopamine(PD)coating,one of the simplest and most versatile surface functionalization method faces challenges in terms of stability and reactivity.In this study,we propose an in situ dynamic reassembly approach to address these challenges.By immersing a pre-deposited PD coating in a strong alkaline solution containing poly(allylamine)hydrochloride(PAH),the dissociated PD oligomers undergo covalent crosslinking in situ,leading to the formation of a reconstructed PDPA coating enriched with stable amino groups through thorough crosslinking.The PDPA coating demonstrates superior chemical and mechanical stability compared to PD,while enhancing multifunctional properties and offering improved surface functional modification potential.The PDPA coating holds promise in materials science,biomedical engineering,and nanotechnology,enabling versatile surface modification and functionalization in extreme conditions.展开更多
AIM:To evaluate the efficacy and safety of one-stitch anastomosis through the skin with bicanalicular silicone tube intubation in repairing of bicanalicular laceration.METHODS:The clinical data of 15 consecutive patie...AIM:To evaluate the efficacy and safety of one-stitch anastomosis through the skin with bicanalicular silicone tube intubation in repairing of bicanalicular laceration.METHODS:The clinical data of 15 consecutive patients with both superior and inferior canalicular laceration in one eye who underwent surgical repair using one-stitch anastomosis through the skin and bicanalicular stent were retrospective studied.All the operations were performed under surgical microscope,5-0 silk sutures were used and were with bicanalicular silicone tube(diameter was 8mm) intubation,for one lacerated canaliculi one-stitch anastomosis through the skin.The stents were left in place for 3 months postoperatively and then removed.The follow-up period was 3-36 months(average 14 months). RESULTS:In 15 patients,13 patients were cured entirely,1 patient was meliorated,1 patient with no effects.All patients had got good recovery of eyelid laceration with no traumatic deformity in eyelid and canthus.Complication was seen in one case,for not followed the doctor’s guidance to come back to hospital to had the suture removed on the 7thday after operation,when he came at the 15thday,the inferior canalicular wall and eyelid skin were corroded by the suture caused 2mm wound,and the inside silicone tube was exposed,a promptly repair with 10-0 nylon suture was done,the wound healed in a week.There were no early tube protrusions and punctal slits in the patients.CONCLUSION:One-stitch anastomosis through the skin with bicanalicular silicone tube intubation is a good method in repair of bicanalicular laceration in one eye,the cut ends can be anastomosed directly,and with excellent cosmetic results,it is acceptable for the patients.For there is no suture remained in the wound permanently,so there is no suture-related granuloma which may cause obstruction or stenosis of canaliculi.It is simple,economical,effective and safe.展开更多
AIM： To investigate the epidemiology and surgical outcomes of old canalicular laceration and analyze the variables impacting on the prognosis of reparation. METHODS： A retrospective review of all old canalicular la...AIM： To investigate the epidemiology and surgical outcomes of old canalicular laceration and analyze the variables impacting on the prognosis of reparation. METHODS： A retrospective review of all old canalicular laceration repairs from Jan. 1, 2008 to Dec. 30, 2015 was performed. Analyzed data included demographics, mechanisms of injury, the time from injury to repair, causes for delayed repair, old associated injuries, the types of surgery, and the effects of repair using canaliculus anastomosis combined with bicanalicular stent intubation. RESULTS： Totally 148 patients with old canalicular laceration received surgical repair and were enrolled. The mean age at presentation was 32.52 years old （ranged from 3 to 63 years old）. The 110 patients （74.32%） were male and 127 patients （85.81%） were adults （__.18 years old）. The old upper, lower, and bicanalicular lacerations were found in 5 （3.38%）, 39 （26.35%）, and 104 patients （70.27%）, respectively. The mechanism of old injury was primarily due to motor vehicle accidents （n=53, 35.81%）. The mean time from injury to repair was 43.61mo （ranged from 1 to 360mo）. Associated old ocular and orbit injuries were found in 65 patients （43.92%）, and chronic dacryocystitis in 18 patients （12.16%）. The main cause of delayed repair was that doctors or patients didn＇t pay attention to the canalicular laceration because of the concurrent severe injuries （n=71, 47.97%）. Totally 136 patients （91.89%） with old canalicular laceration underwent canaliculus anastomosis combined with bicanalicular stent intubation. In all of them, 20 patients （13.51%） were combined with dacryocystorhinostomy. In these cases, 132 patients （97.06%） attained anatomic success, 121 patients （88.97%）reported no epiphora （functional success）, 11 patients （8.09%） reported significant epiphora anesis （functional improvement）, and 4 （2.94%） reported no significant anesis （functional failure）. Rates of anatomic success and functional success were significantly correlated with different canaliculus involved. However, rates of anatomic success and functional success were not significantly affected by the time from injury to repair. CONCLUSION： The canalicular anastomosis combined with bicanalicular stent intubation could act as an effective therapeutics for old canalicular laceration.展开更多
AIM:To assess objective visual quality after presbyopia correction using the Presby MAX monocular mode.METHODS:This prospective,nonrandomized study included 28 eyes from 18 patients(mean age 50.4±5.6 y)who underw...AIM:To assess objective visual quality after presbyopia correction using the Presby MAX monocular mode.METHODS:This prospective,nonrandomized study included 28 eyes from 18 patients(mean age 50.4±5.6 y)who underwent presbyopia correction with the Presby MAX monocular mode.Monocular and binocular visual acuities were evaluated preoperatively,1 d,1 wk,1,3 mo,and 1 y after surgery.Optical quality was analyzed by HartmannShack wavefront aberration supported cornea ablation.Modulation transfer function(MTF)cutoff frequency,Strehl ratio,and objective scattering index(OSI)were analyzed using an optical quality analysis system.RESULTS:One year after surgery,100%and 94.4%of patients achieved binocular uncorrected distance and near visual acuity of 20/25,respectively.At the last visit Spherical aberration and total higher aberration were higher than the corresponding preoperative levels(P<0.001);however,no significant difference was found in MTF,OSI,or Strehl ratio.Transient decreases in OSI and MTF mainly occurred in the nondominant eyes.There was no significant difference in optical quality between the dominant and nondominant eyes,except for spherical aberration and horizontal coma(P<0.05).CONCLUSION:The Presby MAX monocular mode is safe and effective for presbyopia correction.It has little effect on optical quality,though short-term degraded optical quality occurred mainly in the bi-aspheric ablated eyes.展开更多
· AIM: To evaluate the application of ultrasound biomicroscopy(UBM) in the examination of lacrimal canalicular diseases, and to investigate UBM image characteristics of lacrimal canaliculi in disease states.·...· AIM: To evaluate the application of ultrasound biomicroscopy(UBM) in the examination of lacrimal canalicular diseases, and to investigate UBM image characteristics of lacrimal canaliculi in disease states.·METHODS: Sixty cases(63 eyes, 69 canaliculi) of lacrimal canalicular diseases were enrolled that included32 patients(32 eyes, 32 canaliculi) with chronic lacrimal canaliculitis, 18 patients(18 eyes, 18 canaliculi) with previous lacrimal canalicular laceration, 9 patients(12eyes, 18 canaliculi) with congenital absence of lacrimal puncta and canaliculi, and 1 case(1 eye, 1 canaliculus)of canalicular mass. The patients were examined using UBM, and disease-specific features of the UBM images were noted.· RESULTS: UBM imaging of lacrimal canaliculi in chronic canaliculitis patients showed obvious ectasia of the lacrimal canalicular lumen. Dot-like moderate echoic signals were detected on some ectatic lumina of the lacrimal canaliculus. Some lumen-like structures of the lower lacrimal canaliculus were observed in 2(2 eyes, 2canaliculi) of the 9 patients(12 eyes, 18 canaliculi) with congenital absence of the lacrimal canaliculus. Of the 18patients(18 eyes, 18 canaliculi) with previous lacrimal canalicular laceration, the lacerated end on the nasal side of the lacrimal canaliculus was detected only in 14patients(14 eyes, 14 canaliculi).·CONCLUSION: UBM can be used to evaluate lacrimal canalicular diseases and can provide an imaging basis for the diagnosis of lacrimal canalicular diseases.展开更多
AIM： To systematically review the association between complement factors I （CFI） polymorphisms and age- related macular degeneration （AMD） and to explore whether CFI polymorphisms are associated with AMD, METHODS...AIM： To systematically review the association between complement factors I （CFI） polymorphisms and age- related macular degeneration （AMD） and to explore whether CFI polymorphisms are associated with AMD, METHODS： Meta-analysis of articles published from 1995 to January 2015 of articles involved with AMD and polymorphisms of the CFI gene. Eligible data were pooled in a Meta-analysis, analyzing using STATA software （version 12.0）, Review Manager （version 5.2） and different models based on the heterogeneity of effect sizes. Egger＇s test, Begg＇s rank correlation methods were used to evaluate for publication bias.~ RESULTS： Thirteen articles were eligible, describing two loci polymorphisms of the CFI gene （of which 12 articles focus on rs10033900T〉C and 3 articles focus on rs2285714C〉T）. For rs10033900T〉C, the results of our study revealed that having a mutant allele C, TC, CC and TC＋CC was associated with a decreased risk of AMD in all population groups studied （C versus T models, OR=0.84, 95%Ch 0.72-0.99, P=0.04; TC versus TT models OR= 0.89, 95%Ch 0.88-0.99, P=0.04;CC versus ＂1-1＂ models, OR=0.76, 95%Ch 0.60-0.98, P=-0.03; TC＋CC versus TT models, OR=0.81, 95%Ch0.65-0.99, P=0.04）. We found that C allele were related to lower AMD risk in the Caucasian population by subgroup analysis, but there was no association with AMD under the allele and genotypes comparison in Asian studies. For rs2285714 C〉T, the TC, TT genotypes contributed to a higher risk of AMD, compared with the CC carriers and CT＋CC （OR=1.34, 95%Ch 1.09-1.63, P=0.004; OR=1.50, 95%Ch 1.25-1.80, P〈0.0001）. CONCLUSION： This Meta-analysis suggests that CFI rs10033900T〉C and rs2285714C〉T polymorphisms may contribute to AMD.展开更多
AIM: To investigate the one-year refractive outcomes and optical quality following PRESBYOND laser-blended vision(LBV).METHODS: This retrospective study included 20 patients who underwent PRESBYOND treatment between J...AIM: To investigate the one-year refractive outcomes and optical quality following PRESBYOND laser-blended vision(LBV).METHODS: This retrospective study included 20 patients who underwent PRESBYOND treatment between Jan 2019 and Aug 2020. The patients were asked to attend a followup outpatient visit one year after surgery. Distance and near visual acuity as well as subjective refraction were examined. Optical quality was assessed using wavefront-supported custom ablation. A questionnaire evaluating optical quality and satisfaction was completed at the last visit.RESULTS: The average patient age was 48.1±7.4 y(range, 41 to 58 y). The mean preoperative spherical equivalent was-7.59±2.39D. At the one-year follow-up, two eyes(both dominant eyes) lost one line of corrected distance visual acuity(CDVA), while the remaining eyes(38/40) maintained or gained lines of CDVA. The average binocular uncorrected distance visual acuity improved from 0.15±0.03 to 0.90±0.26(decimal vision;P<0.001). The average binocular uncorrected near visual acuity increased from 0.34±0.28 to 0.97±0.07(P<0.001). The spherical aberration was 0.04±0.06 μm in the nondominant eye and 0.09±0.09 μm in the dominant eye(P=0.02). All patients were satisfied with or accepted the outcomes of the surgery. The primary complaints were related to disturbances in night vision and relatively inferior near vision.CONCLUSION: Over the one-year observation period, PRESBYOND is a safe and effective option for presbyopia correction. The optical quality and near vision deserve further investigation.展开更多
AIM:To study the imaging characteristics of lacrimal punctum lesion with optical coherence tomography(OCT),and provide imaging basis for the diagnosis and treatment of lacrimal punctum diseases.METHODS:A total of 25 p...AIM:To study the imaging characteristics of lacrimal punctum lesion with optical coherence tomography(OCT),and provide imaging basis for the diagnosis and treatment of lacrimal punctum diseases.METHODS:A total of 25 patients(28 eyes)with epiphora and lacrimal puncta lesions were enrolled.Lacrimal puncta lesions included:punctum membrane obstruction in 7 cases(9 eyes),punctum agenesis in 1 case(1 eye),a mass protruded from the punctum in 1 case(1 eye),slit puncta in 1 case(1 eye),peri-puncta mass in 2 cases(2 eyes),chronic dacryocystitis in 4 cases(4 eyes),and primary puncta stenosis in 9 cases(10 eyes;3 eyes mild,4 eyes moderate and 3 eyes severe).All patients were examined by slit lamp microscopy and OCT to observe the morphological characteristics of abnormal punctum.RESULTS:Two types of complete membrane obstruction and incomplete membrane obstruction of puncta were observed in OCT images of 7 patients.No lacrimal punctum and lacrimal canalicular cavity were found in 1 case with punctum agenesis.OCT images showed that a narrow lumen remained in the lacrimal puncta in 1 patient with a mass protruded from the punctum.OCT of punctum in a patient with slit punctum after stent placement showed stent and abnormal lacrimal structure.No abnormal intraluminal structure was found in 2 cases of peri-puncta mass after OCT scan,and the lacunar space was narrower than that of the contralateral eye.OCT of puncta in 4 patients with chronic dacryocystitis showed that pus floated in tear with lump-like medium-low reflex.In 9 patients with primary lacrimal puncta stenosis,OCT image could clearly show the changes of puncta lumen in different degrees and shapes.CONCLUSION:OCT is feasible for the examination of pathological punctum,and can provide imaging basis for the diagnosis and treatment of punctum disease.展开更多
AIM:To investigate the variation of IGSF3 gene in three families with congenital absence of lacrimal puncta and canaliculi,and to lay a foundation for further research on the pathogenic gene of congenital lacrimal duc...AIM:To investigate the variation of IGSF3 gene in three families with congenital absence of lacrimal puncta and canaliculi,and to lay a foundation for further research on the pathogenic gene of congenital lacrimal duct agenesis.METHODS:The members of the three families were recruited.The ophthalmologic examinations in details,including slit-lamp biomicroscope,intraocular pressure and fundus examination,etc.were carried out.All patients were checked with paracentesis of puncta membrane and lacrimal duct probing,as well as the computed tomographydacryocystography(CT-DCG).Peripheral blood of 14 participants(3 normal)from three families were collected,4 mL each,for genomic DNA extraction,and 11 exon fragments of IGSF3 gene were amplified and sequenced by polymerase chain reaction(PCR)to determine whether there were IGSF3 genetic variation.RESULTS:A total of 14 members from three families were screened for 4 synonymous variants:c.930 C>T(p.Pro366=),c.1359 T>C(p.Ser709=),c.1797 G>A(p.Ser855=),c.1539 G>A(p.Ser769=),and 6 missense variants:c.l507 G>A(p.Gly759 Ser),c.1783 T>C(p.Trp851 Arg),c.1952 G>T(p.Ser 907 lle),c.3120 C>G(p.Asp1040 Glu),c.3123 C>G(p.Asp1041 Glu),c.3139_3140 insGAC(p.Asp1046_Pro1047 insAsp),and the latter three were only found in two patients with absence of lacrimal puncta and canaliculi combined with congenital osseous nasolacrimal canal obstruction from the first family.CONCLUSION:The same IGSF3 gene mutation c.31393140 insGAC is found in the patients with congenital absence of lacrimal puncta and canaliculi combine with osseous nasolacrimal canal obstruction.展开更多
BACKGROUND： Apoptosis of retinal photoreceptor cells is a commonly pathological characteristic of various eye diseases, while caspase-3 is an important regulating gene and plays a key role in apoptosis. OBJECTIVE： T...BACKGROUND： Apoptosis of retinal photoreceptor cells is a commonly pathological characteristic of various eye diseases, while caspase-3 is an important regulating gene and plays a key role in apoptosis. OBJECTIVE： To measure the expression of caspase-3 mRNA in rats with apoptosis of retinal photoreceptor cells by using real-time fluorescent quantitative polymerase chain reaction and compare with those of the normal rats. DESIGN： Randomized controlled animal study. SETTING： Zhongshan Ophthalmological Center of Sun Yat-sen University. MATERIALS： A total of 36 female SD rats of 50 days old and clean grade and weighing （150±10） g were provided by Experimental Animal Center of Northern Area of Sun Yat-sen University. All rats were randomly divided into normal control group （n =6） and N-methyl-N-nitrosourea （MNU） group （n =30）, and they were observed at 12 hours, 1, 2, 3 and 5 days after model establishment, with 6 rats at each time point. METHODS： The experiment was carried out at Zhongshan Ophthalmological Center, Key Laboratory of Ophthalmology by State Ministry of Education from March to December 2004. Rats in the normal control group were intraperitoneally injected with saline and rats in the MNU group were intraperitoneally injected with 40 mg/kg MNU. And then, retinal photoreceptor injured models were established. At 12 hours, 1, 2, 3 and 5 days after model establishment, the rats were sacrificed for enucleating right eyeballs, isolating retina immediately and extracting total RNA. The expression of caspase-3 mRNA in retina was measured with real-time fluorescent quantitative polymerase chain reaction. MAIN OUTCOME MEASURES： Expression of caspase-3 mRNA in retina of rats in the two groups. RESULTS： A total of 36 SD rats were involved in the final analysis. The expressions of caspase-3 mRNA in the rat retina of both groups at the five time points （12 hours, 1, 2, 3 and 5 days） after model establishment were 1.52×105, 18.35×105, 25.14×105, 29.25×105, 13.72×105 and 12.24×105, respectively. The expression of caspase-3 mRNA in the MNU group increased after 12 hours of intraperitoneal injection, and rose to the top on the 2nd day, which was 19 times as many as that of the normal control group. Then, it decreased gradually and was still 8 times as many as that of the normal control group on the 5th day. CONCLUSION： The expression of caspase-3 mRNA is related to apoptosis of retinal photoreceptor cells, while caspase-3 plays an important role in occurrence and development of apoptosis of retinal photoreceptor cells.展开更多
Dear Editor,We present a case of combined application of CIRCLE software(Carl Zeiss Meditec AG,Germany)and topography-guided laser-assisted in situ keratomileusis(Topo-LASIK)for small-incision lenticule extraction(SMI...Dear Editor,We present a case of combined application of CIRCLE software(Carl Zeiss Meditec AG,Germany)and topography-guided laser-assisted in situ keratomileusis(Topo-LASIK)for small-incision lenticule extraction(SMILE)enhancement.SMILE is a safe,minimally invasive corneal laser surgery using a femtosecond laser to create an extractable stromal lenticule.展开更多
The low drug bioavailability of eye drops challenges the therapy of ocular disorders with high efficacy.One of solutions is to extend the corneal retention and enhance the penetration of drug into cornea.Here we synth...The low drug bioavailability of eye drops challenges the therapy of ocular disorders with high efficacy.One of solutions is to extend the corneal retention and enhance the penetration of drug into cornea.Here we synthesize two fluorophore-conjugated peptide based analogs rich in positive charges(i.e.,NBDFFKK)and with a specific ligand(i.e.,NBD-FFRGD),respectively,to visualize their performances in vitro and in vivo.The peptides both can self-assemble into supramolecular hydrogels with the microstructure of nanofibers.The in vitro experiments exhibit that two peptides are both uniformly distributed in cytoplasm,and the intracellular amount of peptide rich in positive charges is significantly larger than that of peptide with a specific ligand.The living corneal fluorescence shows that two peptides enter the corneal stroma within 15 min,and the peptide rich in positive charges is accumulated more extensively throughout the entire cornea,revealing that the supramolecular hydrogel eye drops penetrate the cornea more efficiently via electrostatic interaction than that via ligand-receptor interaction.This work,as a comparative study of supramolecular hydrogel eye drops on penetrating efficiency,indicates a possible direction for the design of eye drops with efficient corneal penetration.展开更多
Antibacterial agent of activatable photosensitizer not only has the advantages of traditional photosensitizers,such as good curative effect and low resistance,but also has better selectivity for bacteria and lower tox...Antibacterial agent of activatable photosensitizer not only has the advantages of traditional photosensitizers,such as good curative effect and low resistance,but also has better selectivity for bacteria and lower toxicity to normal tissues.Limited reports of activatable photosensitizer can be used to treat drugresistant bacteria.In order to meet this challenge,we designed and synthesized an activatable photosensitizer(Ce-OHOA),which can not only selectively identify methicillin-resistant Staphylococcus aureus(MRSA)with high expression ofβ-lactamase by fluorescence imaging,but also kill MRSA with less than10 times the concentration and 10 times the irradiation dose of CySG-2 reported.Ce-OHOA not only combines the dual functions of fluorescence diagnosis and photodynamic therapy,but also selectively acts on bacteria with high expression ofβ-lactamase and has little toxicity to normal cells.We expect that the study of this activating photosensitizer will provide a new solution for antibacterial photodynamic therapy(aPDT)of drug-resistant bacteria.展开更多
基金Supported by the National Key Research and Development Program of China(No.2022YFC3502503)the Medical and Health Science and Technology Project of the Zhejiang Provincial Health Commission of China(No.2022PY072).
文摘AIM:To explore the repeatability,reproducibility,and agreement in the measurement of the choroidal vascularity index(CVI)for different swept-source optical coherence tomography(OCT)devices and between OCT and OCT angiography(OCTA)images.METHODS:Two swept-source OCT imaging systems,VG200I and Topcon DRI OCT Triton,were used to capture OCT and OCTA images in triplicate.The first and third images were taken by one operator,and the second image was taken by another operator.The built-in software was used to calculate the CVI from the OCTA images(CVI-OCTA),and a custom-designed algorithm was used to calculate the CVI from the OCT images(CVI-OCT).Repeatability and reproducibility were assessed with the intraclass correlation coefficient(ICC),and agreement between devices and between OCT and OCTA were evaluated with Bland-Altman analysis.RESULTS:Sixty-eight eyes from 35 adults(17 females)were included in the analysis.The average age of the participants was 23.6±2.3y,with an average spherical equivalent refraction of-3.08±2.47 D and an average AL of 25.21±1.20 mm.Both OCT devices demonstrated high repeatability and reproducibility in measuring the CVI-OCTA(all ICCs>0.894 across five choroidal regions)and CVI-OCT(all ICCs>0.838).Furthermore,the between-device agreement in measuring the CVI-OCT was good[mean difference(MD)ranging from-2.32%to-3.07%],but that in measuring the CVI-OCTA was poor(MD,1.48%to-7.43%).Additionally,the between-imaging agreement(CVI-OCTA versus CVI-OCT)was poor for both devices(Triton,MD,6.05%to 12.68%;VG200I,MD,6.67%to 12.09%).CONCLUSION:Both OCT devices and the two analytical methods demonstrate good stability.The inter-device consistency of CVI-OCT is good,while the inter-device consistency of CVI-OCTA and the consistency between the two analytical methods in the same device are both poor.
基金Supported by the National Natural Science Foundation of China(No.82171095)the Project of Shanghai Science and Technology(No.23XD1400500)the Research Fund of Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital(No.24QNPY049).
文摘AIM:To evaluate the differences and consistency of vault measurements obtained by Scheimpflug tomography(Pentacam),anterior segment optical coherence tomography(AS-OCT,CASIA II),and ultrasound biomicroscopy(UBM)following implantable collamer lens(ICL)V4c implantation.METHODS:Vault measurements were acquired using three modalities:Pentacam,CASIA II AS-OCT,and UBM.Repeated-measures analysis of variance was used to compare the vault values obtained by the three devices.The correlation and consistency of measurements among the three instruments were assessed using the Pearson correlation coefficient,intraclass correlation coefficient(ICC),and Bland-Altman plots.RESULTS:This retrospective study enrolled 210 myopic eyes of 210 patients(158 women and 52 men)who underwent ICL implantation:108 eyes had a myopic ICL V4c implanted,and 102 eyes had a toric ICL V4c implanted.The mean vault values measured by Pentacam,CASIA II,and UBM were 452.64±204.20μm,538.57±203.54μm,and 560.95±227.54μm,respectively,with statistically significant differences among the three groups(P<0.05).Pearson correlation analysis showed strong positive correlations between vault values measured by different instruments(all P<0.001).ICC results indicated good consistency among the three measurement modalities(all P<0.001).Stratified analysis revealed that when the vault value was≤250μm,the correlation and consistency of measurements across the three instruments were lower than those in the medium and high vault subgroups.CONCLUSION:Vault values measured by Pentacam are lower than those obtained by CASIA II and UBM,with UBM yielding the highest mean vault values.Measurements from the three instruments are not interchangeable but can serve as mutual references due to their significant correlation and good overall consistency.Pentacam and CASIA II demonstrate the highest consistency in vault measurement.Notably,when the vault value is≤250μm,the consistency between Pentacam and the other two instruments decreases significantly.
文摘Self-healing hydrogels utilize inherent intermolecular forces to autonomously heal physical damage resulting from excessive strain,pressure,or tearing.Applying these materials in soft robotics and tissue engineering could be beneficial.On the other hand,their efficacy in stretchable and mechanically resistant circuits is hindered by their limited electrical conductivity.
文摘AIM:To analyze the environmental impact of patient travel for cataract surgery at a German ophthalmology center.METHODS:All cataract surgeries performed between October 23 and October 27,2023,were analyzed,and all patient records were reviewed for follow-up visits.All travel distances were calculated,and the associated emissions were quantified.Additionally,patients’utilization of geographically closer branch practices for follow-up care was evaluated,along with the corresponding effects on travel-related emissions.RESULTS:A total of 69 patients underwent unilateral cataract surgery.The average one-way travel distance was 40.1 km(24.9 mi;SD=23.6 km).Corresponding emissions were 1284.8 kg of greenhouse gas(GHG),2.477 kg of nitrogen oxides,and 0.101 kg of particulates.All patients attended at least two follow-up visits.Conducting followup visits at branch practices reduced travel distance by 49.1%.The associated GHG emissions from all travel were 1984.3 kg.Emissions from follow-up visits were 54.4%higher than those from the surgery itself.Total GHG emissions amounted to 3269.1 kg,with an average of 47.4 kg of GHG per patient for all travel associated with cataract surgery.CONCLUSION:A dense network of branch practices contributes to reducing the carbon footprint of cataract surgery-related patient travel;however,the development of digital health approaches for follow-up care is necessary to further optimize the environmental sustainability of cataract surgery.
基金supported by the Wenzhou Major Scientific and Technological Innovation Project(No.ZY2022020)the Zhejiang Provincial Science and Technology Project for Public Welfare(No.LGF22H140010)the Science and Technology Plan of Wenzhou(No.H20210008)。
文摘In the fight against bacterial infections,it is critical to effectively disrupt biofilms.However,disruption of biofilms becomes exceptionally difficult due to the low permeability of therapeutic agents.Herein,we present a self-propelled nanovesicle(PCL-PLG@CHX)strategy for eliminating biofilms and further expediting the healing of wounds.PCL-PLG@CHX is synthesized by assembling vesicles from amphiphilic polymers,which incorporate both poly-ε-caprolactone and guanidinated-poly-ε-lysine(PCL-PLG)and are infused with chlorhexidine(CHX).Upon application to sites of bacterial infection,PCL-PLG@CHX,abundant in guanidinium structures,effectively accumulates on the negatively charged surface of biofilms.It interacts with reactive oxygen species(ROS)within the biofilm,leading to nitric oxide(NO)production.The generated NO cannot only propel the nanovesicle to penetrate deeper into the biofilm,but also act as a signaling molecule to disperse the biofilm,working in conjunction with the subsequent release of CHX for an enhanced antibacterial impact.Following the eradication of bacteria,the residual guanidine component continues to produce small quantities of NO,facilitating angiogenesis and epithelial growth,thereby accelerating the healing of wounds.Together,our study shows that PCL-PLG@CHX utilizes the potential of guanidine moieties to efficiently break down biofilms and support tissue restoration,tackling the pivotal challenge of biofilm-related diseases.
基金Supported by the Shanghai Natural Science Foundation(No.23ZR1409200).
文摘AIM:To investigate the ocular surface parameters in patients with moderate to high myopia.METHODS:This prospective study was conducted in May 2023,enrolling patients with moderate to high myopia(spherical equivalent refraction≤-3.0 D).After completing the Ocular Surface Disease Index(OSDI)questionnaire,refractive parameters and non-invasive tear film parameters were measured,followed by the Schirmer I test and fluorescein sodium staining.The diagnosis of dry eye disease was based on OSDI score,non-invasive tear breakup time(NIBUT),Schirmer I test,and fluorescein sodium staining results,according to the 2020 Chinese Expert Consensus on Dry Eye.The Mann-Whitney U test was used to compare ocular surface parameters between moderate and high myopia,as well as between patients with and without dry eye.Pearson correlation analysis was employed to assess the relationship between the lipid/muco-aqueous layers and tear film parameters.A general linear mixed model(GLMM)was used to analyze the impact of refractive parameters on ocular surface parameters after adjusting for age and sex.RESULTS:A total of 35 eyes with moderate to high myopia(12 males;mean age,30.30±5.45y)were included in the study.Among them,26 eyes were classified as normal and 9 as dry eye.Of the 9 dry eye cases,7 were observed in the high myopia group(n=18)and 2 in the moderate myopia group(n=17).Among the enrolled patients,those with high myopia demonstrated significantly higher OSDI scores than those with moderate myopia(P=0.0417).Patients with dry eye exhibited significantly shorter interblink intervals(P=0.0081)and higher OSDI scores(P=0.0001)than those without dry eye.Pearson correlation analysis revealed a significant positive correlation between lipid layer thickness(LLT)and tear meniscus height(r=0.395,P=0.023),and a significant negative correlation between the muco-aqueous layer thickness change rate(MALTR)and OSDI score(r=-0.466,P=0.016).After adjusting for age and sex using the GLMM,spherical refraction(SPH,β=-1.802,P=0.048)and axial length(AL,β=2.784,P=0.048)significantly impacted OSDI score.Corneal front astigmatism significantly influenced Schirmer I test results(β=8.377,P=0.024).The difference between central corneal thickness and the thinnest corneal thickness significantly affected LLT(β=-2.294,P=0.026).White-to-white diameter significantly impacted MALTR(β=-81.758,P=0.037).CONCLUSION:In moderate to high myopia,higher SPH and AL correlate with increased dry eye symptoms,which are associated with muco-aqueous and lipid layer alterations.Corneal regularity and diameter also affect tear film dynamics.
基金financial support from the National Natural Science Foundation of China(Nos.82202354,U20A20338,82201247)The Summit Advancement Disciplines of Zhejiang Province(Wenzhou Medical University-Pharmaceutics)the Key R&D Program of Zhejiang Province(No.2021C04019)。
文摘Tumor heterogeneity and diversity significantly undermine the effectiveness of monotherapy.Collaborative therapy emerges as a promising approach to mitigate tumor recurrence resulting from monotherapy.Combining chemodynamic therapy(CDT)with photothermal therapy(PTT)offers a compelling solution for eradicating residual tumor cells post-PTT.In this study,we harness the Fenton-like response facilitated by glucose oxidase(GOD)and the mild hyperthermia induced by polyethyleneimine(PEI)functionalized nitrogen-containing graphene oxide to enhance tumor therapy through a metal-free bionic nanozyme.GOD catalyzes a substantial amount of hydrogen peroxide,and,with the carrier's involvement,triggers a Fenton-like reaction,yielding a wealth of hydroxyl radicals.These hydroxyl radicals effectively target tumor cells following photothermal action,bolstering CDT and culminating in a bidirectional amplification treatment that effectively prevents tumor recurrence and metastasis.This research amalgamates the physical and chemical attributes of nanomaterials with the unique characteristics of the tumor microenvironment,presenting a compelling and efficacious alternative for tumor treatment.
基金supported by the National Natural Science Foundation of China(Nos.82301156,32371377,and 52203131)Leading Talent Project of Guangzhou Development District(No.2020L013)+1 种基金the Zhejiang Provincial Natural Science Foundation of China(No.LTGY23H140001)the Fundamental Research Funds for Wenzhou Medical University(No.KYYW202208)。
文摘Polydopamine(PD)coating,one of the simplest and most versatile surface functionalization method faces challenges in terms of stability and reactivity.In this study,we propose an in situ dynamic reassembly approach to address these challenges.By immersing a pre-deposited PD coating in a strong alkaline solution containing poly(allylamine)hydrochloride(PAH),the dissociated PD oligomers undergo covalent crosslinking in situ,leading to the formation of a reconstructed PDPA coating enriched with stable amino groups through thorough crosslinking.The PDPA coating demonstrates superior chemical and mechanical stability compared to PD,while enhancing multifunctional properties and offering improved surface functional modification potential.The PDPA coating holds promise in materials science,biomedical engineering,and nanotechnology,enabling versatile surface modification and functionalization in extreme conditions.
文摘AIM:To evaluate the efficacy and safety of one-stitch anastomosis through the skin with bicanalicular silicone tube intubation in repairing of bicanalicular laceration.METHODS:The clinical data of 15 consecutive patients with both superior and inferior canalicular laceration in one eye who underwent surgical repair using one-stitch anastomosis through the skin and bicanalicular stent were retrospective studied.All the operations were performed under surgical microscope,5-0 silk sutures were used and were with bicanalicular silicone tube(diameter was 8mm) intubation,for one lacerated canaliculi one-stitch anastomosis through the skin.The stents were left in place for 3 months postoperatively and then removed.The follow-up period was 3-36 months(average 14 months). RESULTS:In 15 patients,13 patients were cured entirely,1 patient was meliorated,1 patient with no effects.All patients had got good recovery of eyelid laceration with no traumatic deformity in eyelid and canthus.Complication was seen in one case,for not followed the doctor’s guidance to come back to hospital to had the suture removed on the 7thday after operation,when he came at the 15thday,the inferior canalicular wall and eyelid skin were corroded by the suture caused 2mm wound,and the inside silicone tube was exposed,a promptly repair with 10-0 nylon suture was done,the wound healed in a week.There were no early tube protrusions and punctal slits in the patients.CONCLUSION:One-stitch anastomosis through the skin with bicanalicular silicone tube intubation is a good method in repair of bicanalicular laceration in one eye,the cut ends can be anastomosed directly,and with excellent cosmetic results,it is acceptable for the patients.For there is no suture remained in the wound permanently,so there is no suture-related granuloma which may cause obstruction or stenosis of canaliculi.It is simple,economical,effective and safe.
基金Supported by the National Natural Science Foundation of China(No.81600767)the National Key Basic Research Program of China(973 Program:No.2013CB967001)Postdoctoral Science Foundation of China(No.2015M582852)
文摘AIM： To investigate the epidemiology and surgical outcomes of old canalicular laceration and analyze the variables impacting on the prognosis of reparation. METHODS： A retrospective review of all old canalicular laceration repairs from Jan. 1, 2008 to Dec. 30, 2015 was performed. Analyzed data included demographics, mechanisms of injury, the time from injury to repair, causes for delayed repair, old associated injuries, the types of surgery, and the effects of repair using canaliculus anastomosis combined with bicanalicular stent intubation. RESULTS： Totally 148 patients with old canalicular laceration received surgical repair and were enrolled. The mean age at presentation was 32.52 years old （ranged from 3 to 63 years old）. The 110 patients （74.32%） were male and 127 patients （85.81%） were adults （__.18 years old）. The old upper, lower, and bicanalicular lacerations were found in 5 （3.38%）, 39 （26.35%）, and 104 patients （70.27%）, respectively. The mechanism of old injury was primarily due to motor vehicle accidents （n=53, 35.81%）. The mean time from injury to repair was 43.61mo （ranged from 1 to 360mo）. Associated old ocular and orbit injuries were found in 65 patients （43.92%）, and chronic dacryocystitis in 18 patients （12.16%）. The main cause of delayed repair was that doctors or patients didn＇t pay attention to the canalicular laceration because of the concurrent severe injuries （n=71, 47.97%）. Totally 136 patients （91.89%） with old canalicular laceration underwent canaliculus anastomosis combined with bicanalicular stent intubation. In all of them, 20 patients （13.51%） were combined with dacryocystorhinostomy. In these cases, 132 patients （97.06%） attained anatomic success, 121 patients （88.97%）reported no epiphora （functional success）, 11 patients （8.09%） reported significant epiphora anesis （functional improvement）, and 4 （2.94%） reported no significant anesis （functional failure）. Rates of anatomic success and functional success were significantly correlated with different canaliculus involved. However, rates of anatomic success and functional success were not significantly affected by the time from injury to repair. CONCLUSION： The canalicular anastomosis combined with bicanalicular stent intubation could act as an effective therapeutics for old canalicular laceration.
基金Supported by National Natural Science Foundation of China(No.81770955)National Natural Science Foundation of China for Young Scholar(No.81600762)+1 种基金Project of Shanghai Science and Technology(No.17411950200)Joint Research Project of New Frontier Technology in Municipal Hospitals(No.SHDC12018103)。
文摘AIM:To assess objective visual quality after presbyopia correction using the Presby MAX monocular mode.METHODS:This prospective,nonrandomized study included 28 eyes from 18 patients(mean age 50.4±5.6 y)who underwent presbyopia correction with the Presby MAX monocular mode.Monocular and binocular visual acuities were evaluated preoperatively,1 d,1 wk,1,3 mo,and 1 y after surgery.Optical quality was analyzed by HartmannShack wavefront aberration supported cornea ablation.Modulation transfer function(MTF)cutoff frequency,Strehl ratio,and objective scattering index(OSI)were analyzed using an optical quality analysis system.RESULTS:One year after surgery,100%and 94.4%of patients achieved binocular uncorrected distance and near visual acuity of 20/25,respectively.At the last visit Spherical aberration and total higher aberration were higher than the corresponding preoperative levels(P<0.001);however,no significant difference was found in MTF,OSI,or Strehl ratio.Transient decreases in OSI and MTF mainly occurred in the nondominant eyes.There was no significant difference in optical quality between the dominant and nondominant eyes,except for spherical aberration and horizontal coma(P<0.05).CONCLUSION:The Presby MAX monocular mode is safe and effective for presbyopia correction.It has little effect on optical quality,though short-term degraded optical quality occurred mainly in the bi-aspheric ablated eyes.
文摘· AIM: To evaluate the application of ultrasound biomicroscopy(UBM) in the examination of lacrimal canalicular diseases, and to investigate UBM image characteristics of lacrimal canaliculi in disease states.·METHODS: Sixty cases(63 eyes, 69 canaliculi) of lacrimal canalicular diseases were enrolled that included32 patients(32 eyes, 32 canaliculi) with chronic lacrimal canaliculitis, 18 patients(18 eyes, 18 canaliculi) with previous lacrimal canalicular laceration, 9 patients(12eyes, 18 canaliculi) with congenital absence of lacrimal puncta and canaliculi, and 1 case(1 eye, 1 canaliculus)of canalicular mass. The patients were examined using UBM, and disease-specific features of the UBM images were noted.· RESULTS: UBM imaging of lacrimal canaliculi in chronic canaliculitis patients showed obvious ectasia of the lacrimal canalicular lumen. Dot-like moderate echoic signals were detected on some ectatic lumina of the lacrimal canaliculus. Some lumen-like structures of the lower lacrimal canaliculus were observed in 2(2 eyes, 2canaliculi) of the 9 patients(12 eyes, 18 canaliculi) with congenital absence of the lacrimal canaliculus. Of the 18patients(18 eyes, 18 canaliculi) with previous lacrimal canalicular laceration, the lacerated end on the nasal side of the lacrimal canaliculus was detected only in 14patients(14 eyes, 14 canaliculi).·CONCLUSION: UBM can be used to evaluate lacrimal canalicular diseases and can provide an imaging basis for the diagnosis of lacrimal canalicular diseases.
基金Supported by the Scientific Research Project of Traditional Chinese Medicine of Health and Family Planning Commission of Chongqing (ZY20150243)
文摘AIM： To systematically review the association between complement factors I （CFI） polymorphisms and age- related macular degeneration （AMD） and to explore whether CFI polymorphisms are associated with AMD, METHODS： Meta-analysis of articles published from 1995 to January 2015 of articles involved with AMD and polymorphisms of the CFI gene. Eligible data were pooled in a Meta-analysis, analyzing using STATA software （version 12.0）, Review Manager （version 5.2） and different models based on the heterogeneity of effect sizes. Egger＇s test, Begg＇s rank correlation methods were used to evaluate for publication bias.~ RESULTS： Thirteen articles were eligible, describing two loci polymorphisms of the CFI gene （of which 12 articles focus on rs10033900T〉C and 3 articles focus on rs2285714C〉T）. For rs10033900T〉C, the results of our study revealed that having a mutant allele C, TC, CC and TC＋CC was associated with a decreased risk of AMD in all population groups studied （C versus T models, OR=0.84, 95%Ch 0.72-0.99, P=0.04; TC versus TT models OR= 0.89, 95%Ch 0.88-0.99, P=0.04;CC versus ＂1-1＂ models, OR=0.76, 95%Ch 0.60-0.98, P=-0.03; TC＋CC versus TT models, OR=0.81, 95%Ch0.65-0.99, P=0.04）. We found that C allele were related to lower AMD risk in the Caucasian population by subgroup analysis, but there was no association with AMD under the allele and genotypes comparison in Asian studies. For rs2285714 C〉T, the TC, TT genotypes contributed to a higher risk of AMD, compared with the CC carriers and CT＋CC （OR=1.34, 95%Ch 1.09-1.63, P=0.004; OR=1.50, 95%Ch 1.25-1.80, P〈0.0001）. CONCLUSION： This Meta-analysis suggests that CFI rs10033900T〉C and rs2285714C〉T polymorphisms may contribute to AMD.
基金Supported by National Natural Science Foundation of China(No.81770955)National Natural Science Foundation of China for Young Scholars(No.82000929)+6 种基金Project of Shanghai Science and Technology(No.20410710100)Clinical Research Plan of SHDC(No.SHDC2020CR1043B)Project of Shanghai Xuhui District Science and Technology(No.2020-015)Project of Shanghai Xuhui District Science and Technology(No.XHLHGG202104)Shanghai Engineering Research Center of Laser and Autostereoscopic 3D for Vision Care(No.20DZ2255000)Shanghai Sailing Program(No.20YF1405000)。
文摘AIM: To investigate the one-year refractive outcomes and optical quality following PRESBYOND laser-blended vision(LBV).METHODS: This retrospective study included 20 patients who underwent PRESBYOND treatment between Jan 2019 and Aug 2020. The patients were asked to attend a followup outpatient visit one year after surgery. Distance and near visual acuity as well as subjective refraction were examined. Optical quality was assessed using wavefront-supported custom ablation. A questionnaire evaluating optical quality and satisfaction was completed at the last visit.RESULTS: The average patient age was 48.1±7.4 y(range, 41 to 58 y). The mean preoperative spherical equivalent was-7.59±2.39D. At the one-year follow-up, two eyes(both dominant eyes) lost one line of corrected distance visual acuity(CDVA), while the remaining eyes(38/40) maintained or gained lines of CDVA. The average binocular uncorrected distance visual acuity improved from 0.15±0.03 to 0.90±0.26(decimal vision;P<0.001). The average binocular uncorrected near visual acuity increased from 0.34±0.28 to 0.97±0.07(P<0.001). The spherical aberration was 0.04±0.06 μm in the nondominant eye and 0.09±0.09 μm in the dominant eye(P=0.02). All patients were satisfied with or accepted the outcomes of the surgery. The primary complaints were related to disturbances in night vision and relatively inferior near vision.CONCLUSION: Over the one-year observation period, PRESBYOND is a safe and effective option for presbyopia correction. The optical quality and near vision deserve further investigation.
基金Supported by High-level Talents Training Foundation of the Armed Police Force(No.GCCRC-02-2017)。
文摘AIM:To study the imaging characteristics of lacrimal punctum lesion with optical coherence tomography(OCT),and provide imaging basis for the diagnosis and treatment of lacrimal punctum diseases.METHODS:A total of 25 patients(28 eyes)with epiphora and lacrimal puncta lesions were enrolled.Lacrimal puncta lesions included:punctum membrane obstruction in 7 cases(9 eyes),punctum agenesis in 1 case(1 eye),a mass protruded from the punctum in 1 case(1 eye),slit puncta in 1 case(1 eye),peri-puncta mass in 2 cases(2 eyes),chronic dacryocystitis in 4 cases(4 eyes),and primary puncta stenosis in 9 cases(10 eyes;3 eyes mild,4 eyes moderate and 3 eyes severe).All patients were examined by slit lamp microscopy and OCT to observe the morphological characteristics of abnormal punctum.RESULTS:Two types of complete membrane obstruction and incomplete membrane obstruction of puncta were observed in OCT images of 7 patients.No lacrimal punctum and lacrimal canalicular cavity were found in 1 case with punctum agenesis.OCT images showed that a narrow lumen remained in the lacrimal puncta in 1 patient with a mass protruded from the punctum.OCT of punctum in a patient with slit punctum after stent placement showed stent and abnormal lacrimal structure.No abnormal intraluminal structure was found in 2 cases of peri-puncta mass after OCT scan,and the lacunar space was narrower than that of the contralateral eye.OCT of puncta in 4 patients with chronic dacryocystitis showed that pus floated in tear with lump-like medium-low reflex.In 9 patients with primary lacrimal puncta stenosis,OCT image could clearly show the changes of puncta lumen in different degrees and shapes.CONCLUSION:OCT is feasible for the examination of pathological punctum,and can provide imaging basis for the diagnosis and treatment of punctum disease.
基金Supported by High-Level Talents Training Foundation of the Armed Police Force(No.GCCRC-02)。
文摘AIM:To investigate the variation of IGSF3 gene in three families with congenital absence of lacrimal puncta and canaliculi,and to lay a foundation for further research on the pathogenic gene of congenital lacrimal duct agenesis.METHODS:The members of the three families were recruited.The ophthalmologic examinations in details,including slit-lamp biomicroscope,intraocular pressure and fundus examination,etc.were carried out.All patients were checked with paracentesis of puncta membrane and lacrimal duct probing,as well as the computed tomographydacryocystography(CT-DCG).Peripheral blood of 14 participants(3 normal)from three families were collected,4 mL each,for genomic DNA extraction,and 11 exon fragments of IGSF3 gene were amplified and sequenced by polymerase chain reaction(PCR)to determine whether there were IGSF3 genetic variation.RESULTS:A total of 14 members from three families were screened for 4 synonymous variants:c.930 C>T(p.Pro366=),c.1359 T>C(p.Ser709=),c.1797 G>A(p.Ser855=),c.1539 G>A(p.Ser769=),and 6 missense variants:c.l507 G>A(p.Gly759 Ser),c.1783 T>C(p.Trp851 Arg),c.1952 G>T(p.Ser 907 lle),c.3120 C>G(p.Asp1040 Glu),c.3123 C>G(p.Asp1041 Glu),c.3139_3140 insGAC(p.Asp1046_Pro1047 insAsp),and the latter three were only found in two patients with absence of lacrimal puncta and canaliculi combined with congenital osseous nasolacrimal canal obstruction from the first family.CONCLUSION:The same IGSF3 gene mutation c.31393140 insGAC is found in the patients with congenital absence of lacrimal puncta and canaliculi combine with osseous nasolacrimal canal obstruction.
基金the National Natural Science Foundation of China, No. 30300467
文摘BACKGROUND： Apoptosis of retinal photoreceptor cells is a commonly pathological characteristic of various eye diseases, while caspase-3 is an important regulating gene and plays a key role in apoptosis. OBJECTIVE： To measure the expression of caspase-3 mRNA in rats with apoptosis of retinal photoreceptor cells by using real-time fluorescent quantitative polymerase chain reaction and compare with those of the normal rats. DESIGN： Randomized controlled animal study. SETTING： Zhongshan Ophthalmological Center of Sun Yat-sen University. MATERIALS： A total of 36 female SD rats of 50 days old and clean grade and weighing （150±10） g were provided by Experimental Animal Center of Northern Area of Sun Yat-sen University. All rats were randomly divided into normal control group （n =6） and N-methyl-N-nitrosourea （MNU） group （n =30）, and they were observed at 12 hours, 1, 2, 3 and 5 days after model establishment, with 6 rats at each time point. METHODS： The experiment was carried out at Zhongshan Ophthalmological Center, Key Laboratory of Ophthalmology by State Ministry of Education from March to December 2004. Rats in the normal control group were intraperitoneally injected with saline and rats in the MNU group were intraperitoneally injected with 40 mg/kg MNU. And then, retinal photoreceptor injured models were established. At 12 hours, 1, 2, 3 and 5 days after model establishment, the rats were sacrificed for enucleating right eyeballs, isolating retina immediately and extracting total RNA. The expression of caspase-3 mRNA in retina was measured with real-time fluorescent quantitative polymerase chain reaction. MAIN OUTCOME MEASURES： Expression of caspase-3 mRNA in retina of rats in the two groups. RESULTS： A total of 36 SD rats were involved in the final analysis. The expressions of caspase-3 mRNA in the rat retina of both groups at the five time points （12 hours, 1, 2, 3 and 5 days） after model establishment were 1.52×105, 18.35×105, 25.14×105, 29.25×105, 13.72×105 and 12.24×105, respectively. The expression of caspase-3 mRNA in the MNU group increased after 12 hours of intraperitoneal injection, and rose to the top on the 2nd day, which was 19 times as many as that of the normal control group. Then, it decreased gradually and was still 8 times as many as that of the normal control group on the 5th day. CONCLUSION： The expression of caspase-3 mRNA is related to apoptosis of retinal photoreceptor cells, while caspase-3 plays an important role in occurrence and development of apoptosis of retinal photoreceptor cells.
基金Supported by the Shanghai Rising-Star Program(No.21QA1401500)Shanghai Natural Science Foundation(No.23ZR1409200).
文摘Dear Editor,We present a case of combined application of CIRCLE software(Carl Zeiss Meditec AG,Germany)and topography-guided laser-assisted in situ keratomileusis(Topo-LASIK)for small-incision lenticule extraction(SMILE)enhancement.SMILE is a safe,minimally invasive corneal laser surgery using a femtosecond laser to create an extractable stromal lenticule.
基金supported by National Natural Science Foundation of China(Nos.82102215 and 82372129)Wenzhou Medical University Scientific Research Fund(No.KYQD20210602)。
文摘The low drug bioavailability of eye drops challenges the therapy of ocular disorders with high efficacy.One of solutions is to extend the corneal retention and enhance the penetration of drug into cornea.Here we synthesize two fluorophore-conjugated peptide based analogs rich in positive charges(i.e.,NBDFFKK)and with a specific ligand(i.e.,NBD-FFRGD),respectively,to visualize their performances in vitro and in vivo.The peptides both can self-assemble into supramolecular hydrogels with the microstructure of nanofibers.The in vitro experiments exhibit that two peptides are both uniformly distributed in cytoplasm,and the intracellular amount of peptide rich in positive charges is significantly larger than that of peptide with a specific ligand.The living corneal fluorescence shows that two peptides enter the corneal stroma within 15 min,and the peptide rich in positive charges is accumulated more extensively throughout the entire cornea,revealing that the supramolecular hydrogel eye drops penetrate the cornea more efficiently via electrostatic interaction than that via ligand-receptor interaction.This work,as a comparative study of supramolecular hydrogel eye drops on penetrating efficiency,indicates a possible direction for the design of eye drops with efficient corneal penetration.
基金financially supported by the National Natural Science Foundation of China (No.21977081)Zhejiang Provincial Natural Science of Foundation of China (No.LQ21H190006)。
文摘Antibacterial agent of activatable photosensitizer not only has the advantages of traditional photosensitizers,such as good curative effect and low resistance,but also has better selectivity for bacteria and lower toxicity to normal tissues.Limited reports of activatable photosensitizer can be used to treat drugresistant bacteria.In order to meet this challenge,we designed and synthesized an activatable photosensitizer(Ce-OHOA),which can not only selectively identify methicillin-resistant Staphylococcus aureus(MRSA)with high expression ofβ-lactamase by fluorescence imaging,but also kill MRSA with less than10 times the concentration and 10 times the irradiation dose of CySG-2 reported.Ce-OHOA not only combines the dual functions of fluorescence diagnosis and photodynamic therapy,but also selectively acts on bacteria with high expression ofβ-lactamase and has little toxicity to normal cells.We expect that the study of this activating photosensitizer will provide a new solution for antibacterial photodynamic therapy(aPDT)of drug-resistant bacteria.
