Bone tissue engineering provides a promising strategy for the treatment of bone defects.Nonetheless,the clinical utilization of biomaterial-based scaffolds is constrained by their inadequate mechanical strength and ab...Bone tissue engineering provides a promising strategy for the treatment of bone defects.Nonetheless,the clinical utilization of biomaterial-based scaffolds is constrained by their inadequate mechanical strength and absence of osteo-inductive properties.Here,we proposed to endow nano-scaffold(NS)constructed by coaxial electrospinning technique with enhanced osteogenic bioactivities and mechanical properties by incorporating biocompatible magnetic iron oxide nanoparticles(IONPs)and icaritin(ICA).Four types of nano-scaffolds(NS,ICA@NS,NS-IONPs and ICA@NS-IONPs)were prepared.The incorporation of ICA and IONPs minimally impact their surface morphological and chemical properties.IONPs enhanced the mechanical properties of NS scaffolds,including hardness,tensile strength,and elastic modulus.In vitro assessments demonstrated that ICA@NS-IONPs exhibited enhanced osteogenic bioactivities towards mouse calvarial pre-osteoblast cell line MC3T3-E1 as evidenced by detecting the alkaline phosphatase(ALP)activity level,expressions of osteogenesis-related genes and proteins as well as mineralized nodule formation.Mechanistic investigations revealed that MEK/ERK(MAP kinase-ERK kinase(MEK)/extracellularsignal-regulated kinase(ERK))signaling pathway could offer a plausible explanation for the osteogenic differentiation of MC3T3-E1 cells induced by ICA@NS-IONPs.Furthermore,the implantation of nano-scaffolds in rat skull defects exhibited a substantial improvement in in vivo bone regeneration.Therefore,IONPs and ICA incorporated coaxial electrospinning nano-scaffolds present a novel strategy for the optimization of scaffolds for bone tissue engineering.展开更多
Ⅰ. GEOLOGICAL BACKGROUND Covering an area of 1200 km^2, the Wugongshan migmatite-granite batholith is located on the northwestern margin of South China Caledonian folded region and the southern flank of Pingxiang-Gua...Ⅰ. GEOLOGICAL BACKGROUND Covering an area of 1200 km^2, the Wugongshan migmatite-granite batholith is located on the northwestern margin of South China Caledonian folded region and the southern flank of Pingxiang-Guangfeng deep fault. It is characterized by the trinity of meta-sediments,展开更多
The gabbro in Yejili, Neimenggu, is located about 100 km northeast of the largest Cu-Ni sulphide ore in Jinchuan, Gansu, which is on the upwarped district of margin of Alashan Platform southwest of Sino-Korean Block. ...The gabbro in Yejili, Neimenggu, is located about 100 km northeast of the largest Cu-Ni sulphide ore in Jinchuan, Gansu, which is on the upwarped district of margin of Alashan Platform southwest of Sino-Korean Block. The gabbro intruded into the Precambrian contact zone of plagiogranite, moyite and pladorite. Its strike on the surface is along the lines of 45° northeast, about 3.6 km in length and 2.1 km in width. Several small ore veins of ilmenite and magnetite have been discovered in展开更多
基金supported by the National Basic Research Program of China(Nos.2021YFA1201404,and 2019YFA0210103)the National Natural Science Foundation of China(Nos.32271413,and 82272492)+1 种基金Natural Science Foundation of Jiangsu Province(No.BK20232023)Science program of Jiangsu Province Administration for Market Regulation(No.KJ2024010).
文摘Bone tissue engineering provides a promising strategy for the treatment of bone defects.Nonetheless,the clinical utilization of biomaterial-based scaffolds is constrained by their inadequate mechanical strength and absence of osteo-inductive properties.Here,we proposed to endow nano-scaffold(NS)constructed by coaxial electrospinning technique with enhanced osteogenic bioactivities and mechanical properties by incorporating biocompatible magnetic iron oxide nanoparticles(IONPs)and icaritin(ICA).Four types of nano-scaffolds(NS,ICA@NS,NS-IONPs and ICA@NS-IONPs)were prepared.The incorporation of ICA and IONPs minimally impact their surface morphological and chemical properties.IONPs enhanced the mechanical properties of NS scaffolds,including hardness,tensile strength,and elastic modulus.In vitro assessments demonstrated that ICA@NS-IONPs exhibited enhanced osteogenic bioactivities towards mouse calvarial pre-osteoblast cell line MC3T3-E1 as evidenced by detecting the alkaline phosphatase(ALP)activity level,expressions of osteogenesis-related genes and proteins as well as mineralized nodule formation.Mechanistic investigations revealed that MEK/ERK(MAP kinase-ERK kinase(MEK)/extracellularsignal-regulated kinase(ERK))signaling pathway could offer a plausible explanation for the osteogenic differentiation of MC3T3-E1 cells induced by ICA@NS-IONPs.Furthermore,the implantation of nano-scaffolds in rat skull defects exhibited a substantial improvement in in vivo bone regeneration.Therefore,IONPs and ICA incorporated coaxial electrospinning nano-scaffolds present a novel strategy for the optimization of scaffolds for bone tissue engineering.
基金Project supported by the National Natural Science Foundation of China
文摘Ⅰ. GEOLOGICAL BACKGROUND Covering an area of 1200 km^2, the Wugongshan migmatite-granite batholith is located on the northwestern margin of South China Caledonian folded region and the southern flank of Pingxiang-Guangfeng deep fault. It is characterized by the trinity of meta-sediments,
基金Project supported by the National Natural Science Foundation of Chinathe Foundation of National Laboratory for Metalliferous OreMetallogenetic Action of Nanjing University.
文摘The gabbro in Yejili, Neimenggu, is located about 100 km northeast of the largest Cu-Ni sulphide ore in Jinchuan, Gansu, which is on the upwarped district of margin of Alashan Platform southwest of Sino-Korean Block. The gabbro intruded into the Precambrian contact zone of plagiogranite, moyite and pladorite. Its strike on the surface is along the lines of 45° northeast, about 3.6 km in length and 2.1 km in width. Several small ore veins of ilmenite and magnetite have been discovered in
