OBJECTIVE： To study the effects of berberine on ac- tivity and mRNA expression of N-acetyltransferase in human lung cancer cell line A549. METHODS： N-acetyltransferase antibodies wereprepared. The human lung cancer ...OBJECTIVE： To study the effects of berberine on ac- tivity and mRNA expression of N-acetyltransferase in human lung cancer cell line A549. METHODS： N-acetyltransferase antibodies wereprepared. The human lung cancer A549 cells were cultivated randomly in the wells of culture plate, and divided into the control group, and berberine 0.0008, 0.008, 0.08, 0.8 and 1.6 mM treatment groups, with 3 wells for each group. 24 h later, A549 cells in each group were collected respectively, the content of N-acetyltransferase was detected by Flow cytometry, and the mRNA expression of N-acetyltransferase was observed by reverse tran- scription polymerase chain reaction. RESULTS The N-acetyltransferase content in hu- man lung cancer A549 cells decreased with the in- creasing of berberine concentration, significantly lower than that in the control group （P〈O.05 or P〈 0.001）; and the mRNA expression of N-acetyltrans- ferase also decreased with the increasing of berber- ine concentration, significantly lower in Huanglian- su treatment groups （P〈0.O01）. CONCLUSION： Berberine can inhibit the activity of N-acetyltransferase in human lung cancer cell line A549, and shows negative correlations of dose and time in a certain extent. The inhibited gene expres- sion of N-acetyltransferase in human lung cancer A549 cell may probably represent one of the mech- anisms for its antineoplastic effect.展开更多
Constipation is correlated with diminished cognitive function,revealing a possible rectum-brain connection.In this counter-balanced crossover trial,13 elite triathletes underwent a Stroop test to assess cognitive func...Constipation is correlated with diminished cognitive function,revealing a possible rectum-brain connection.In this counter-balanced crossover trial,13 elite triathletes underwent a Stroop test to assess cognitive function and executive control.The Stroop test was conducted both with and without magnesium oxide intake,with a 1-week washout period between sessions.Oxygenation and blood distribution during the cognitive challenge were measured using Near-Infrared Spectroscopy(NIRS).Measurements were taken in both the prefrontal brain and the sub-navel region,where the highest glucose uptake was detected under the 18F-fluorodeoxyglucose Positron Emission Tomography(PET)scan.A significant reduction in completion time for the Stroop test was observed after defecation compared to the non-defecated condition(non-defecation:[27.1±1.1]s;non-magnesium defecation:[24.4±0.9]s;magnesium defecation:[23.4±0.8]s,p<0.05).Stroop test performance was improved in all(100%,13/13)of the participants after magnesium-induced defecation and most(69%,9/13)of the participants after non-magnesium-induced defecation.While no alterations in oxygenation and blood distribution were observed in the prefrontal brain during the Stroop test,decreased oxygenation levels were observed in the sub-navel region under both defecated conditions,without significant changes in blood distribution(p<0.05).This data suggests an acute increase in oxygen consumption at this specific region.The result of this study suggests an unexplored causal link between the state of the rectum and cognitive performance.Magnesium supplementation to improved rectal emptying presents a novel application for optimizing cognitive function in athletes navigating intricate racing conditions.展开更多
The multifactorial and multistage pathogenesis of hepatocellular carcinoma(HCC)has fascinated a wide spectrum of scientists for decades.While a number of major risk factors have been identified,their mechanistic roles...The multifactorial and multistage pathogenesis of hepatocellular carcinoma(HCC)has fascinated a wide spectrum of scientists for decades.While a number of major risk factors have been identified,their mechanistic roles in hepatocarcinogenesis still need to be elucidated.Many tumor suppressor genes(TSGs)have been identified as being involved in HCC.These TSGs can be classified into two groups depending on the situation with respect to allelic mutation/loss in the tumors:the recessive TSGs with two required mutated alleles and the haploinsufficient TSGs with one required mutated allele.Hepatitis B virus(HBV)is one of the most important risk factors associated with HCC.Although mice cannot be infected with HBV due to the narrow host range of HBV and the lack of a proper receptor,one advantage of mouse models for HBV/HCC research is the numerous and powerfulgenetic tools that help investigate the phenotypic effects of viral proteins and allow the dissection of the dose-dependent action of TSGs.Here,we mainly focus on the application of mouse models in relation to HBV-associated HCC and on TSGs that act either in a recessive or in a haploinsufficient manner.Discoveries obtained using mouse models will have a great impact on HCC translational medicine.展开更多
基金Supported by Xiamen Science and Technology Key Program Plan Grant(No.3502Z20100006)Scientific Research Starting Foundation for New Teacher of Xiamen University(No.ZK1014)
文摘OBJECTIVE： To study the effects of berberine on ac- tivity and mRNA expression of N-acetyltransferase in human lung cancer cell line A549. METHODS： N-acetyltransferase antibodies wereprepared. The human lung cancer A549 cells were cultivated randomly in the wells of culture plate, and divided into the control group, and berberine 0.0008, 0.008, 0.08, 0.8 and 1.6 mM treatment groups, with 3 wells for each group. 24 h later, A549 cells in each group were collected respectively, the content of N-acetyltransferase was detected by Flow cytometry, and the mRNA expression of N-acetyltransferase was observed by reverse tran- scription polymerase chain reaction. RESULTS The N-acetyltransferase content in hu- man lung cancer A549 cells decreased with the in- creasing of berberine concentration, significantly lower than that in the control group （P〈O.05 or P〈 0.001）; and the mRNA expression of N-acetyltrans- ferase also decreased with the increasing of berber- ine concentration, significantly lower in Huanglian- su treatment groups （P〈0.O01）. CONCLUSION： Berberine can inhibit the activity of N-acetyltransferase in human lung cancer cell line A549, and shows negative correlations of dose and time in a certain extent. The inhibited gene expres- sion of N-acetyltransferase in human lung cancer A549 cell may probably represent one of the mech- anisms for its antineoplastic effect.
基金supported by a grant from University of Taipei,Taipei,Taiwan China.
文摘Constipation is correlated with diminished cognitive function,revealing a possible rectum-brain connection.In this counter-balanced crossover trial,13 elite triathletes underwent a Stroop test to assess cognitive function and executive control.The Stroop test was conducted both with and without magnesium oxide intake,with a 1-week washout period between sessions.Oxygenation and blood distribution during the cognitive challenge were measured using Near-Infrared Spectroscopy(NIRS).Measurements were taken in both the prefrontal brain and the sub-navel region,where the highest glucose uptake was detected under the 18F-fluorodeoxyglucose Positron Emission Tomography(PET)scan.A significant reduction in completion time for the Stroop test was observed after defecation compared to the non-defecated condition(non-defecation:[27.1±1.1]s;non-magnesium defecation:[24.4±0.9]s;magnesium defecation:[23.4±0.8]s,p<0.05).Stroop test performance was improved in all(100%,13/13)of the participants after magnesium-induced defecation and most(69%,9/13)of the participants after non-magnesium-induced defecation.While no alterations in oxygenation and blood distribution were observed in the prefrontal brain during the Stroop test,decreased oxygenation levels were observed in the sub-navel region under both defecated conditions,without significant changes in blood distribution(p<0.05).This data suggests an acute increase in oxygen consumption at this specific region.The result of this study suggests an unexplored causal link between the state of the rectum and cognitive performance.Magnesium supplementation to improved rectal emptying presents a novel application for optimizing cognitive function in athletes navigating intricate racing conditions.
基金Supported by Research grants from the Ministry of Science and Technology(MOST)in Taiwan,No.NSC99-2628-B-010-001-MY3,MOST 103-2321-B-010-003,MOST 103-2633-H-010-001,MOST 103-2633-B-400-002 and MOST104-3011-B-010-001a grant from the Ministry of Education,Aim for the Top University Plan
文摘The multifactorial and multistage pathogenesis of hepatocellular carcinoma(HCC)has fascinated a wide spectrum of scientists for decades.While a number of major risk factors have been identified,their mechanistic roles in hepatocarcinogenesis still need to be elucidated.Many tumor suppressor genes(TSGs)have been identified as being involved in HCC.These TSGs can be classified into two groups depending on the situation with respect to allelic mutation/loss in the tumors:the recessive TSGs with two required mutated alleles and the haploinsufficient TSGs with one required mutated allele.Hepatitis B virus(HBV)is one of the most important risk factors associated with HCC.Although mice cannot be infected with HBV due to the narrow host range of HBV and the lack of a proper receptor,one advantage of mouse models for HBV/HCC research is the numerous and powerfulgenetic tools that help investigate the phenotypic effects of viral proteins and allow the dissection of the dose-dependent action of TSGs.Here,we mainly focus on the application of mouse models in relation to HBV-associated HCC and on TSGs that act either in a recessive or in a haploinsufficient manner.Discoveries obtained using mouse models will have a great impact on HCC translational medicine.
