Background:Although the benefits of Huang-Lian-Jie-Du-Decoction(HLJDD)on type 2 diabetes mellitus are noted,the material base and action mechanism remain unknown.This paper aim is to reveal the material base and actio...Background:Although the benefits of Huang-Lian-Jie-Du-Decoction(HLJDD)on type 2 diabetes mellitus are noted,the material base and action mechanism remain unknown.This paper aim is to reveal the material base and action mechanism of HLJDD against type 2 diabetes mellitus in a system pharmacology framework.Methods:The compounds in HLJDD were first retrieved from the Traditional Chinese Medicine Systems Pharmacology database and analysis platform.Once retrieved,they were fed into the SwissTargetPrediction database to predict the interacting targets.Meanwhile,a human expression profile dataset was analyzed in the Gene Expression Omnibus database,and subsequently,the differentially expressed genes were compared to the HLJDD-related targets.We conducted a protein-protein interaction analysis,Kyoto Encyclopedia of Genes and Genomes pathway analysis,and Gene Ontology analysis to identify the potential active compounds and targets.Lastly,to verify the binding affinities of those compounds and targets,we performed molecular docking.Results:We obtained 15 key compounds,such as quercetin,epiberberine,and berberine,and 10 hub genes,such as IκB kinase-βand phosphatidylinositol 3-kinase regulatory subunit alpha.The top 10 enriched pathways were also found to be tightly related to type 2 diabetes mellitus,including insulin resistance and FoxO signaling pathway.Moreover,all the key compounds were found to bind well to the hub genes.Particularly for the target of IκB kinase-β,11 out of 15 compounds bound to it with energies of<−9.0 kcal/mol.Conclusion:In summary,15 key compounds of HLJDD may affect type 2 diabetes mellitus development by multiple genes such as IκB kinase-βand phosphatidylinositol 3-kinase regulatory subunit alpha and signaling pathways such as insulin resistance and FoxO signaling pathway.展开更多
The chemical constituents of Cortex Moutan charcoal were studied in depth, and their coagulation activity was screened. The chemical constituents were isolated by polyamide, silica gel and Sephadex G-Sepharose chromat...The chemical constituents of Cortex Moutan charcoal were studied in depth, and their coagulation activity was screened. The chemical constituents were isolated by polyamide, silica gel and Sephadex G-Sepharose chromatography purification, and their structures were identified by NMR spectroscopy and other analyses. A total of 10 compounds were obtained and identified as follows： 3-（2-furan）-l-（2-hydroxy-4-methoxy-phenyl）-2-propylene ketone （1）, 2-（2,5-hydroxy-4-methyl phenyl） propionate ethyl ester （2）, methyl tetradec-5-enoate （3）, 1,4-diethylcyclohexane （4）, catechol （5）, methyl-4-hydroxybenzoate （6）, 3-hydroxy- 2-methyl-4-pyrone （7）, 3,8-dihydroxy-2-metyl-chromone （8）, 3β-hydroxy-olean-12-en （9）, 1-monolinolein （10）. Among them, compounds 1 and 2 were new natural products, and 3-10 were isolated from the Cortex Moutan charcoal for the first time. The potential coagulation activities of compounds were evaluated, and compounds 2, 9 and 10 had strong hemostatic effects. While compounds 1 and 8 could significantly activate blood circulation.展开更多
In this article, we report on a room-temperature humidity sensing device using graphene oxide (GO) thin films synthesized by chemical exfoliation. Changes in the device conductivity are measured for varying relative h...In this article, we report on a room-temperature humidity sensing device using graphene oxide (GO) thin films synthesized by chemical exfoliation. Changes in the device conductivity are measured for varying relative humidity in the experimental chamber. Experiments are carried out for relative humidity varying from 30% to 95%. We observe a difference in the results obtained for low relative humidity (50%), and propose a sensing mechanism to explain this difference. Although the sensor exhibits some hysteresis at high relative humidities, a method to “reset” the sensor is also proposed. The sensing device has high sensitivity and fast response time.展开更多
基金the Natural Science Foundation of Guangdong Province(No.2016A030313837).
文摘Background:Although the benefits of Huang-Lian-Jie-Du-Decoction(HLJDD)on type 2 diabetes mellitus are noted,the material base and action mechanism remain unknown.This paper aim is to reveal the material base and action mechanism of HLJDD against type 2 diabetes mellitus in a system pharmacology framework.Methods:The compounds in HLJDD were first retrieved from the Traditional Chinese Medicine Systems Pharmacology database and analysis platform.Once retrieved,they were fed into the SwissTargetPrediction database to predict the interacting targets.Meanwhile,a human expression profile dataset was analyzed in the Gene Expression Omnibus database,and subsequently,the differentially expressed genes were compared to the HLJDD-related targets.We conducted a protein-protein interaction analysis,Kyoto Encyclopedia of Genes and Genomes pathway analysis,and Gene Ontology analysis to identify the potential active compounds and targets.Lastly,to verify the binding affinities of those compounds and targets,we performed molecular docking.Results:We obtained 15 key compounds,such as quercetin,epiberberine,and berberine,and 10 hub genes,such as IκB kinase-βand phosphatidylinositol 3-kinase regulatory subunit alpha.The top 10 enriched pathways were also found to be tightly related to type 2 diabetes mellitus,including insulin resistance and FoxO signaling pathway.Moreover,all the key compounds were found to bind well to the hub genes.Particularly for the target of IκB kinase-β,11 out of 15 compounds bound to it with energies of<−9.0 kcal/mol.Conclusion:In summary,15 key compounds of HLJDD may affect type 2 diabetes mellitus development by multiple genes such as IκB kinase-βand phosphatidylinositol 3-kinase regulatory subunit alpha and signaling pathways such as insulin resistance and FoxO signaling pathway.
基金National Natural Science Foundation of China(Grant No.81473352)Guangdong Province Innovation Training Project(Grant No.201610573040)Guangzhou Science and Technology Bureau Foundation(Grant No.201707010170)
文摘The chemical constituents of Cortex Moutan charcoal were studied in depth, and their coagulation activity was screened. The chemical constituents were isolated by polyamide, silica gel and Sephadex G-Sepharose chromatography purification, and their structures were identified by NMR spectroscopy and other analyses. A total of 10 compounds were obtained and identified as follows： 3-（2-furan）-l-（2-hydroxy-4-methoxy-phenyl）-2-propylene ketone （1）, 2-（2,5-hydroxy-4-methyl phenyl） propionate ethyl ester （2）, methyl tetradec-5-enoate （3）, 1,4-diethylcyclohexane （4）, catechol （5）, methyl-4-hydroxybenzoate （6）, 3-hydroxy- 2-methyl-4-pyrone （7）, 3,8-dihydroxy-2-metyl-chromone （8）, 3β-hydroxy-olean-12-en （9）, 1-monolinolein （10）. Among them, compounds 1 and 2 were new natural products, and 3-10 were isolated from the Cortex Moutan charcoal for the first time. The potential coagulation activities of compounds were evaluated, and compounds 2, 9 and 10 had strong hemostatic effects. While compounds 1 and 8 could significantly activate blood circulation.
文摘In this article, we report on a room-temperature humidity sensing device using graphene oxide (GO) thin films synthesized by chemical exfoliation. Changes in the device conductivity are measured for varying relative humidity in the experimental chamber. Experiments are carried out for relative humidity varying from 30% to 95%. We observe a difference in the results obtained for low relative humidity (50%), and propose a sensing mechanism to explain this difference. Although the sensor exhibits some hysteresis at high relative humidities, a method to “reset” the sensor is also proposed. The sensing device has high sensitivity and fast response time.
