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An Intelligent Vibration System for Concrete in Nuclear Power Engineering
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作者 Yuzhong Han Shiliang Ji +3 位作者 Pu Chen Yulin Liu Weihong Dong Pengyu Zhang 《Journal of World Architecture》 2025年第3期72-78,共7页
In nuclear power engineering,the quality requirements for concrete are extremely stringent.Concrete structures must exhibit high durability to withstand the effects of nuclear radiation,chemical corrosion,and environm... In nuclear power engineering,the quality requirements for concrete are extremely stringent.Concrete structures must exhibit high durability to withstand the effects of nuclear radiation,chemical corrosion,and environmental changes.In particular,nuclear power projects impose higher design standards and safety requirements regarding concrete density.Traditional manual vibration and visual inspection methods are difficult to ensure the required level of concrete compaction.This paper presents an intelligent vibration technology for concrete in nuclear power engineering to enhance construction quality and efficiency.By integrating intelligent sensors,control systems,and data processing algorithms,the technology enables real-time monitoring and evaluation of the vibration process.Results show that intelligent vibration technology effectively ensures the density and uniformity of concrete in nuclear power engineering,thereby improving structural safety and reliability. 展开更多
关键词 Nuclear Power Engineering Intelligent Vibration System Smart Sensor DENSITY Concrete construction
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Human umbilical cord mesenchymal stem cells to treat spinal cord injury in the early chronic phase: study protocol for a prospective, multicenter, randomized, placebo-controlled, single-blinded clinical trial 被引量:10
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作者 Yang Yang Mao Pang +5 位作者 Yu-Yong Chen Liang-Ming Zhang Hao Liu Jun Tan Bin Liu Li-Min Rong 《Neural Regeneration Research》 SCIE CAS CSCD 2020年第8期1532-1538,共7页
Human umbilical cord mesenchymal stem cells(hUC-MSCs)support revascularization,inhibition of inflammation,regulation of apoptosis,and promotion of the release of beneficial factors.Thus,they are regarded as a promisin... Human umbilical cord mesenchymal stem cells(hUC-MSCs)support revascularization,inhibition of inflammation,regulation of apoptosis,and promotion of the release of beneficial factors.Thus,they are regarded as a promising candidate for the treatment of intractable spinal cord injury(SCI).Clinical studies on patients with early chronic SCI(from 2 months to 1 year post-injury),which is clinically common,are rare;therefore,we will conduct a prospective,multicenter,randomized,placebo-controlled,single-blinded clinical trial at the Third Affiliated Hospital of Sun Yat-sen University,West China Hospital of Sichuan University,and Shanghai East Hospital,Tongji University School of Medicine,China.The trial plans to recruit 66 early chronic SCI patients.Eligible patients will undergo randomization at a 2:1 ratio to two arms:the observation group and the control group.Subjects in the observation group will receive four intrathecal transplantations of stem cells,with a dosage of 1×106/kg,at one calendar month intervals.Subjects in the control group will receive intrathecal administrations of 10 mL sterile normal saline in place of the stem cell transplantations.Clinical safety will be assessed by the analysis of adverse events and laboratory tests.The American Spinal Injury Association(ASIA)total score will be the primary efficacy endpoint,and the secondary efficacy outcomes will be the following:ASIA impairment scale,International Association of Neural Restoration-Spinal Cord Injury Functional Rating Scale,muscle tension,electromyogram,cortical motor and cortical sensory evoked potentials,residual urine volume,magnetic resonance imaging–diffusion tensor imaging,T cell subtypes in serum,neurotrophic factors and inflammatory factors in both serum and cerebrospinal fluid.All evaluations will be performed at 1,3,6,and 12 months following the final intrathecal administration.During the entire study procedure,all adverse events will be reported as soon as they are noted.This trial is designed to evaluate the clinical safety and efficacy of subarachnoid transplantation of hUC-MSCs to treat early chronic SCI.Moreover,it will establish whether cytotherapy can ameliorate local hostile microenvironments,promote tracking fiber regeneration,and strengthen spinal conduction ability,thus improving overall motor,sensory,and micturition/defecation function in patients with early chronic SCI.This study was approved by the Stem Cell Research Ethics Committee of the Third Affiliated Hospital of Sun Yat-sen University,China(approval No.[2018]-02)on March 30,2018,and was registered with ClinicalTrials.gov(registration No.NCT03521323)on April 12,2018.The revised trial protocol(protocol version 4.0)was approved by the Stem Cell Research Ethics Committee of the Third Affiliated Hospital of Sun Yat-sen University,China(approval No.[2019]-10)on February 25,2019,and released on ClinicalTrials.gov on April 29,2019. 展开更多
关键词 clinical study early chronic phase efficacy human umbilical cord mesenchymal stem cell multicenter trial prospective study randomized controlled trial safety spinal cord injury study protocol
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Comparative study of heat transfer and pressure drop for curved-twisted tubes utilized in chemical engineering
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作者 Morteza Khoshvaght-Aliabadi Saber Deldar +1 位作者 Shafiqur Rehman Ashkan Alimoradi 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2021年第12期53-64,共12页
Increasing importance of heat transfer in chemical engineering science causes that investigation in the field of enhancement techniques is always one of the up-to-date topics for study.In the current comparative analy... Increasing importance of heat transfer in chemical engineering science causes that investigation in the field of enhancement techniques is always one of the up-to-date topics for study.In the current comparative analysis,the thermal enhancement and friction penalty are explored numerically for curved tubes via twisted configuration.To accomplish this,three common geometries namely helical,serpentine,and Archimedes spiral,are considered at different coil-pitches and twist-pitches as well as five Reynolds numbers in the laminar flow regime.The results exhibit noticeable enhancements(up to 60%)in the thermal performance of the twisted cases as compared to the smooth cases.The highest increases are recorded for the serpentine case,followed by the helical and spiral cases.It is found that these enhancements vary via coil-pitch and twist-pitch.Increasing coil-pitch and twist-pitch augments both heat transfer coefficient and pressure drop in all curved-twisted tubes,however,the effects of twist-pitch are more pronounced.To predict Nusselt number and friction factor,new correlations are also proposed.The maximum deviations of the predicted results compared to the simulated data are within±5%. 展开更多
关键词 Computational fluid dynamics Heat transfer Laminar flow Curved-twisted tubes Comparative study
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Near-infrared fluorescent probe with large Stokes shift and long emission wavelength for rapid diagnosis of lung cancer via aerosol inhalation delivery
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作者 Wenping Dong Mo Ma +4 位作者 Jingkang Li Lanlan Xu Dejiang Gao Pinyi Ma Daqian Song 《Chinese Chemical Letters》 2025年第5期532-535,共4页
The aggressive nature and high mortality rate of lung cancer underscore the imperative need for early diagnosis of the disease.Thus,aminopeptidase N(APN),a potential biomarker for lung cancer,should be thoroughly inve... The aggressive nature and high mortality rate of lung cancer underscore the imperative need for early diagnosis of the disease.Thus,aminopeptidase N(APN),a potential biomarker for lung cancer,should be thoroughly investigated in this context.This report describes the development of HA-apn,a novel near-infrared fluorescent probe,specifically engineered for the sensitive detection of endogenous APN.Characterized by its high selectivity,straightforward molecular architecture,and suitable optical properties,including a long-wavelength emission at 835 nm and a large Stokes shift of 285 nm,HA-apn had high efficacy in identifying overexpressed APN in tumor cells,which shows its potential in pinpointing malignancies.To further validate its applicability and effectiveness in facilitating the direct and enhanced visualization of pulmonary alterations,an in situ lung cancer mouse model was employed.Notably,HAapn was applied for in vivo imaging of APN activity in the lung cancer mouse model receiving the probe through aerosol inhalation,and rapid and precise diagnostic results were achieved within 30min postadministration.Overall,HA-apn can be applied as an effective,non-intrusive tool for the rapid and accurate detection of pulmonary conditions. 展开更多
关键词 NEAR-INFRARED Fluorescent probe Large Stokes shift In situ lung cancer Rapid diagnosis
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Mechanisms and strategies for corrosion control of additively manufactured magnesium alloys
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作者 Mara Cristina Lopes de Oliveira Renato Altobelli Antunes 《Journal of Magnesium and Alloys》 2025年第10期4764-4794,共31页
Additive manufacturing(AM)of magnesium alloys is under intense research activity.Practical engineering applications demand the development of parts with adequate corrosion resistance for their safe use.It is imperativ... Additive manufacturing(AM)of magnesium alloys is under intense research activity.Practical engineering applications demand the development of parts with adequate corrosion resistance for their safe use.It is imperative to understand the corrosion mechanisms of AM-processed Mg alloys,their correlation with processing parameters,and microstructural aspects.The present review explores this topic.A thorough assessment of the current literature on the AM methods of Mg alloys was undertaken,focusing on the main corrosion mechanisms,and their correlation with the microstructure,process defects,post-processing operations,and alloy composition.The opportunities to enhance the knowledge in this field are discussed. 展开更多
关键词 Magnesium alloys Additive manufacturing CORROSION
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Hybrid MNLTP Texture Descriptor and PDCNN-Based OCT Image Classification for Retinal Disease Detection
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作者 Jahida Subhedar Anurag Mahajan +1 位作者 Shabana Urooj Neeraj Kumar Shukla 《Computers, Materials & Continua》 2025年第2期2831-2847,共17页
Retinal Optical Coherence Tomography (OCT) images, a non-invasive imaging technique, have become a standard retinal disease detection tool. Due to disease, there are morphological and textural changes in the layers of... Retinal Optical Coherence Tomography (OCT) images, a non-invasive imaging technique, have become a standard retinal disease detection tool. Due to disease, there are morphological and textural changes in the layers of the retina. Classifying OCT images is challenging, as the morphological manifestations of different diseases may be similar. The OCT images capture the reflectivity characteristics of the retinal tissues. Retinal diseases change the reflectivity property of retinal tissues, resulting in texture variations in OCT images. We propose a hybrid approach to OCT image classification in which the Convolution Neural Network (CNN) model is trained using Multiple Neighborhood Local Ternary Pattern (MNLTP) texture descriptors of the OCT images dataset for a robust disease prediction system. Parallel deep CNN (PDCNN) is proposed to improve feature representation and generalizability. The MNLTP-PDCNN model is tested on two publicly available datasets. The parameter values Accuracy, Precision, Recall, and F1-Score are calculated. The best accuracy obtained specifying the model’s overall performance is 93.98% and 99% for the NEH and OCT2017 datasets, respectively. With the proposed architecture, comparable performance is obtained with a subset of the original OCT2017 data set and a comparatively smaller number of trainable parameters (1.6 million, 1.8 million, and 2.3 million for a single CNN branch, two parallel CNN branches, and three parallel network branches, respectively), compared to off-the-shelf CNN models. Hence, the proposed approach is suitable for real-time OCT image classification systems with fast training of the CNN model and reduced memory requirement for computations. 展开更多
关键词 Local ternary pattern texture descriptor optical coherence tomography parallel CNN multiple neighborhood
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Iron sulfide mineral/polylactic acid mixotrophic biofilter for simultaneous nitrate and phosphate removal
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作者 Yuwei Zhang Xueyu Liu +6 位作者 Shiyang Wang Rong A Shujie Qian Yaquan Liang Yonglan Tian Dongyang Wei Huayong Zhang 《Journal of Environmental Sciences》 2025年第10期56-67,共12页
Heterotrophic denitrification based on polylactic acid(PLAHD)can remove nitrate effectively,but it is expensive and can't remove phosphate.Autotrophic denitrification based on iron sulfide(ISAD)can simultaneously ... Heterotrophic denitrification based on polylactic acid(PLAHD)can remove nitrate effectively,but it is expensive and can't remove phosphate.Autotrophic denitrification based on iron sulfide(ISAD)can simultaneously remove nitrate and phosphate cost-effectively,but its nitrate rate is slow.So,iron sulfide mineral/polylactic acid mixotrophic biofilter(ISPLAB)was constructed to combine advantages of ISAD and PLAHD.ISPLAB achieved nitrogen and phosphorus removal rates of 98.04%and 94.12%,respectively,at a hydraulic retention time(HRT)of 24 h.The study also revealed that controlling molecular weight(MW)of PLA improved the release of soluble organic matter;adding iron sulfide enhanced the hydrolysis of PLA and precipitated PO_(4)^(3-) of Fe^(2+)/Fe^(3+),thereby facilitated simultaneous nitrogen and phosphorus removal.Microbial community analysis resulted that denitrifying bacterias(Phaeodactylibacter and Methylotenera),sulfur-reducing bacterias(Hyphomicrobium),sulfur-oxidizing bacteria(Denitratisoma),iron-reducing bacteria(Romboutsia)and hydrolyzed bacterias(norank_f_norank_o_1-20 and norank_f_Caldilineaceae)coexisted in the ISPLAB system.Organics and iron sulfide drived the denitrification process in ISPLAB. 展开更多
关键词 PLA FES Nutrient pollution Mixotrophic denitrification Simultaneous nitrate and phosphate removal
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Spatiotemporal evolution of net ecosystem productivity and the driving mechanisms in Horqin Sandy Land,China
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作者 XU Xiaona ZHANG Huayong 《Journal of Arid Land》 2026年第1期34-55,共22页
Vegetation in terrestrial ecosystems as a carbon sink is a crucial factor in mitigating global warming and reaching carbon neutrality targets,although the drivers of net ecosystem productivity(NEP)under combined human... Vegetation in terrestrial ecosystems as a carbon sink is a crucial factor in mitigating global warming and reaching carbon neutrality targets,although the drivers of net ecosystem productivity(NEP)under combined human and environmental pressures remain poorly understood.In this study,we analyzed the spatiotemporal evolution of NEP in the Horqin Sandy Land,China from 2000 to 2020,and observed the variation in NEP across different land use types.We further identified and quantified the effects of human activities,topographical features,climatic conditions,and soil properties on NEP through the application of structural equation modeling(SEM)and boosted regression trees(BRT).The results showed that the multi-year average NEP ranged from–137.79 to 461.96 g C/m^(2) in the Horqin Sandy Land,with 88.21%of the area showing a significant increasing trend.Among different land use types,forestland exhibited the highest NEP values,followed by cropland,grassland,impervious land,and unused land.The NEP in carbon sink areas was primarily regulated by potential evapotranspiration(negatively correlated)and precipitation(positively correlated).Slope was identified as the most significant positive determinant in carbon source areas.Forestland exhibited climate–topography interactions driving NEP,whereas cropland and grassland relied on temperature;unused land and impervious land were susceptible to land use/cover change and human footprint.This study has significant implications for maintaining the carbon sink function and promoting ecological engineering programs that aim to enhance the capacity of terrestrial carbon sinks in the semi-arid agro-pastoral ecotone. 展开更多
关键词 net ecosystem productivity(NEP) land use/cover change(LUCC) carbon sink climate change human activities structural equation modeling(SEM) semi-arid agro-pastoral ecotone
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Novel Strategy for Synthesis of High Quality CdTe Nanocrystals in Aqueous Solution 被引量:21
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作者 YAN Yu-xi MU Ying +5 位作者 FENG Guo-dong ZHANG Lei ZHU Lin-lin XU Ling YANG Rui JIN Qin-han 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2008年第1期8-14,共7页
A novel aqueous route for the synthesis of high-quality CdTe nanocrystals(NCs) is presented in this article. With both glutathione(GSH) and cysteine[n(GSH):n(cysteine)=1:3] as stabilizers, high-quality CdTe ... A novel aqueous route for the synthesis of high-quality CdTe nanocrystals(NCs) is presented in this article. With both glutathione(GSH) and cysteine[n(GSH):n(cysteine)=1:3] as stabilizers, high-quality CdTe NCs with controllable photoluminescence(PL) wavelength from 500 to 630 nm can be prepared within 4 h. As-prepared CdTe NCs show higher photoluminescence quantum yields(PLQY) compared with CdTe NCs prepared via other aqueous methods. When the fluorescent emission peak appeared in orange-red window, the PLQY reaches 70% or above at room temperature without any post-preparative treatment. 展开更多
关键词 CdTe nanocrystal Quantum dot synthesis GSH CYSTEINE X-Ray photoelectron spectroscopy
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Redesign of a conformal boundary recovery algorithm for 3D Delaunay triangulation 被引量:10
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作者 CHEN Jian-jun ZHENG Yao 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2006年第12期2031-2042,共12页
Boundary recovery is one of the main obstacles in applying the Delaunay criterion to mesh generation. A stan- dard resolution is to add Steiner points directly at the intersection positions between missing boundaries ... Boundary recovery is one of the main obstacles in applying the Delaunay criterion to mesh generation. A stan- dard resolution is to add Steiner points directly at the intersection positions between missing boundaries and triangulations. We redesign the algorithm with the aid of some new concepts, data structures and operations, which make its implementation routine. Furthermore, all possible intersection cases and their solutions are presented, some of which are seldom discussed in the litera- ture. Finally, numerical results are presented to evaluate the performance of the new algorithm. 展开更多
关键词 Boundary recovery Delaunay triangulation Mesh generation Data structure
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Mechanical force drives the polarization and orientation of cells 被引量:7
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作者 Shijie He Xiaomeng Li Baohua Ji 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2019年第2期275-288,共14页
Collective cells are organized to form specific patterns which play important roles in various physiological and pathological processes,such as tissue morphogenesis,wound healing,and cancer invasion.Compared to single... Collective cells are organized to form specific patterns which play important roles in various physiological and pathological processes,such as tissue morphogenesis,wound healing,and cancer invasion.Compared to single cell behaviors,which has been intensively studied from many aspects(cell migration,adhesion,polarization,proliferation,etc.)and at various scales(molecular,subcellular,and cellular),the multiple cell behaviors are relatively less understood,particularly in a quantitative manner.In this paper,we will present our recent studies of collective polarization and orientation of multiple cells through both experimental measurement and theoretical modeling,including those cell behaviors on/in 2D and 3D substrate/tissue.We find that the collective cell behaviors,including polarization,alignment and migration are closely related to local stress states in cell layer or tissue,which demonstrate the crucial roles of mechanical forces in the living organisms.Specifically,the cells prefer to polarize and align along the maximum principal stress in the cell layer,and the aspect ratio of cell increases with the in-plane maximum shear stress,suggesting that the maximum shear stress is the underlying driving force of cell polarization and orientation.This theory of stress-driven cell behaviors of polarization and orientation provides a new perspective for understanding cell behaviors in living organisms and the guideline for tissue engineering in biomedical applications. 展开更多
关键词 COLLECTIVE CELL BEHAVIORS CELL POLARIZATION CELL alignment Quantification CELL MECHANOSENSING
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Seed Zone Properties and Crop Performance as Affected by Three No-Till Seeders for Permanent Raised Beds in Arid Northwest China 被引量:5
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作者 HE Jin LI Hong-wen +4 位作者 Allen David McHugh WANG Qing-jie LI Hui Rabi Gautam Rasaily Khokan Kumer Sarker 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第10期1654-1664,共11页
The no-till seeders of various soil opener configurations have been shown to produce various soil physical responses in relation to soil and climate conditions, thus affecting crop performance in permanent raised beds... The no-till seeders of various soil opener configurations have been shown to produce various soil physical responses in relation to soil and climate conditions, thus affecting crop performance in permanent raised beds (PRB) systems. This is particularly important in arid Northwest China where large volumes of residue are retained on the soil surface after harvest. In Zhangye, Gansu Province, China, a field trial assessed the effects of three typical (powered-chopper, powered-cutter and powered-disc) PRB no-till seeders and one traditional seeder on soil disturbance, residue cover index, bulk density, fuel consumption, plant growth, and subsequent yield. In general, seedbed conditions and crop performance for PRB no- till seeders seeded plots were better than for traditional seeded plots. In PRB cropping system, the powered-chopper seeder decreased mean soil disturbance and increased residue cover index compared to powered-disc and -cutter seeders. However, the results indicated that soil bulk density was 2.3-4.8% higher, soil temperature was 0.2-0.6℃ lower, and spring wheat emergence was 3.2-4.7% less. This was attributed to greater levels of residue cover and firmer seedbeds. Spring maize and wheat performance in the powered-cutter and -disc treatments was better (non-significant) than powered- chopper treatment. So powered disc no-till seeder, which generally provided the best planting condition and the highest yield, appeared to be the suitable seeder in heavy residue cover conditions. Considering the precision requirements for soil disturbance and residue cover, the powered strip-chopping no-till seeder could be a suitable option for PRB cropping system in Northwest China. Although these results are preliminary, they are still valuable for the design and selection of no-till seeders for PRB cropping systems in arid Northwest China. 展开更多
关键词 no-till seeder permanent raised beds crop growth SEEDBED yield
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Dose optimization of intrathecal administration of human umbilical cord mesenchymal stem cells for the treatment of subacute incomplete spinal cord injury 被引量:9
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作者 Ting-Ting Cao Huan Chen +5 位作者 Mao Pang Si-Si Xu Hui-Quan Wen Bin Liu Li-Min Rong Mang-Mang Li 《Neural Regeneration Research》 SCIE CAS CSCD 2022年第8期1785-1794,共10页
Human umbilical cord mesenchymal stem cells(hUC-MSCs)are a promising candidate for spinal cord injury(SCI)repair owing to their advantages of low immunogenicity and easy accessibility over other MSC sources.However,mo... Human umbilical cord mesenchymal stem cells(hUC-MSCs)are a promising candidate for spinal cord injury(SCI)repair owing to their advantages of low immunogenicity and easy accessibility over other MSC sources.However,modest clinical efficacy hampered the progression of these cells to clinical translation.This discrepancy may be due to many variables,such as cell source,timing of implantation,route of administration,and relevant efficacious cell dose,which are critical factors that affect the efficacy of treatment of patients with SCI.Previously,we have evaluated the safety and efficacy of 4×10^(6) hUC-MSCs/kg in the treatment of subacute SCI by intrathecal implantation in rat models.To search for a more accurate dose range for clinical translation,we compared the effects of three different doses of hUC-MSCs-low(0.25×10^(6) cells/kg),medium(1×10^(6) cells/kg)and high(4×10^(6) cells/kg)-on subacute SCI repair through an elaborate combination of behavioral analyses,anatomical analyses,magnetic resonance imaging-diffusion tensor imaging(MRI-DTI),biotinylated dextran amine(BDA)tracing,electrophysiology,and quantification of mRNA levels of ion channels and neurotransmitter receptors.Our study demonstrated that the medium dose,but not the low dose,is as efficient as the high dose in producing the desired therapeutic outcomes.Furthermore,partial restoration of theγ-aminobutyric acid type A(GABAA)receptor expression by the effective doses indicates that GABAA receptors are possible candidates for therapeutic targeting of dormant relay pathways in injured spinal cord.Overall,this study revealed that intrathecal implantation of 1×10^(6) hUC-MSCs/kg is an alternative approach for treating subacute SCI. 展开更多
关键词 effective dose human umbilical cord mesenchymal stem cells intrathecal implantation ion channels neurotransmitter receptors spinal cord injury subacute spinal cord injury γ-aminobutyric acid type A(GABA_(A))receptors
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Review on Application of Artificial Intelligence in Civil Engineering 被引量:3
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作者 Youqin Huang Jiayong Li Jiyang Fu 《Computer Modeling in Engineering & Sciences》 SCIE EI 2019年第12期845-875,共31页
In last few years,big data and deep learning technologies have been successfully applied in various fields of civil engineering with the great progress of machine learning techniques.However,until now,there has been n... In last few years,big data and deep learning technologies have been successfully applied in various fields of civil engineering with the great progress of machine learning techniques.However,until now,there has been no comprehensive review on its applications in civil engineering.To fill this gap,this paper reviews the application and development of artificial intelligence in civil engineering in recent years,including intelligent algorithms,big data and deep learning.Through the work of this paper,the research direction and difficulties of artificial intelligence in civil engineering for the past few years can be known.It is shown that the studies of artificial intelligence in civil engineering mainly focus on structural maintenance and management,and the design optimization. 展开更多
关键词 Artificial intelligence civil engineering intelligent algorithms big data deep learning structural maintenance
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Development of Double Antibody Sandwich ELISA for Detection of Duck or Goose Flavivirus 被引量:4
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作者 NIU Hui-min HUANG Xin-mei +8 位作者 HAN Kai-kai LIU Yu-zhuo ZHAO Dong-min ZHANG Jing-feng LIU Fei LI Tong-tong ZHOU Xiao-bo LI Xiang-rui LI Yin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2013年第9期1638-1643,共6页
In order to establish double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detection of duck or goose flavivirus, polyclonal antibody against the flavivirus strain JS804 in geese and monoclonal... In order to establish double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detection of duck or goose flavivirus, polyclonal antibody against the flavivirus strain JS804 in geese and monoclonal antibody against the E protein of flavivirus strain JS804 in geese were used as the capture antibody and detection antibody, respectively. The optimal dilution of the capture antibody and detecting antibody capable of detecting the flavivirus strain JS804 in geese were 1:3 200 and 1:160 in the check-board titration, respectively. The reaction time of sample was 1 h, and the optimal working dilution of HRP-labeled goat-anti-mouse IgG was 1:10 000. The positive standard value was 0.247 (OD450.m). The geese flavivirus could be detected at a minimal concentration of 1.875 μg mL^-1. The ELISA had no cross-reaction with Newcastle disease virus (NDV), Avian influenza virus (AIV), Infectious bronchitis virus (IBV), Infectious bursal disease virus (IBDV), Duck hepatitis virus (DHV), and Gosling plague virus (GPV). Twenty clinical samples were detected by the DAS-ELISA and RT-PCR respectively, with the agreement rate of 75%. The results revealed that the DAS-ELISA possessed favorable specificity and higher sensitivity, indicating a suitable method for rapid detection of the duck or goose flavivirus. 展开更多
关键词 GOOSE FLAVIVIRUS double antibody sandwich ELISA monoclonal antibodies
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The ICM method with objective function transformed by variable discrete condition for continuum structure 被引量:21
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作者 Yunkang Sui Xirong Peng 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2006年第1期68-75,共8页
ICM (Independent Continuous Mapping) method can solve topological optimization problems with the minimized weight as the objective and subjected to displacement constraints. To get a clearer topological configuratio... ICM (Independent Continuous Mapping) method can solve topological optimization problems with the minimized weight as the objective and subjected to displacement constraints. To get a clearer topological configuration, by introducing the discrete condition of topological variables and integrating with the original objective, an optimal model with multi-objectives is formulated to make the topological variables approach 0 or 1 as near as possible, and the model reduces the effect of deleting rate on the result. The image-filtering method is employed to eliminate the checkerboard patterns and mesh dependence that occurred in the topology optimization of a continuum structure. The computational efficiency is enhanced through selecting quasi-active displacement constraints and a design region. Numerical examples indicate that this algorithm is robust and practicable, though the number of iterations is slightly increased with respect to the original algorithm. 展开更多
关键词 Structural topological optimization ICM method Checkerboard patterns Mesh dependence Thedeleting rate
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Additive-free porous assemblies of Ti3C2Tx by freeze-drying for high performance supercapacitors 被引量:3
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作者 Yue Yang Lili Wu +5 位作者 Lu Li Shuangyan Lin Lina Bai Xinzhi Ma Zhitao Shao Xitian Zhang 《Chinese Chemical Letters》 SCIE CAS CSCD 2020年第4期1034-1038,共5页
Ti3C2Tx has shown great potential in energy storage filed,but the restacking between Ti3C2Tx nanosheets seriously hampers the maximization of its capacitance.In this study,we rationally designed and synthesized porous... Ti3C2Tx has shown great potential in energy storage filed,but the restacking between Ti3C2Tx nanosheets seriously hampers the maximization of its capacitance.In this study,we rationally designed and synthesized porous Ti3C2Tx assemblies without any additive by introducing ice as spacers using a facile freeze-drying method.The porous Ti3C2Tx assemblies have a three-dimensional network structure,which consists of ultra large Ti3C2Tx lamellar walls and lots of macro-and mesopores.It has been proven that there are more-O groups on the surface of the porous Ti3C2Tx assemblies than the Ti3C2Tx film.The porous Ti3C2Tx assemblies deliver a maximum areal capacitance of 1668 mF/cm^2 when the mass loading is 8.4 mg/cm^2,an optimized specific capacitance of 247.2 F/g when the mass loading is 5.3 mg/cm^2,and87%capacitance retention over 10000 cycles.The symmetric solid-state supercapacitors based on the porous Ti3C2Tx assemblies show an areal capacitance of 355.8 mF/cm^2,the maximum power density of50 mW/cm^2 and an outstanding flexibility under different deformation. 展开更多
关键词 MXenes Ti3C2Tx SUPERCAPACITOR FREEZE-DRYING Additive-free
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Efficacy and Safety of Washed Microbiota Transplantation to Treat Patients with Mild-to-Severe COVID-19 and Suspected of Having Gut Microbiota Dysbiosis: Study Protocol for a Randomized Controlled Trial 被引量:3
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作者 Li-hao WU Zhi-ning YE +5 位作者 Ping PENG Wen-rui XIE Jia-ting XU Xue-yuan ZHANG Harry Hua-xiang XIA Xing-xiang HE 《Current Medical Science》 SCIE CAS 2021年第6期1087-1095,共9页
Objective:Coronavirus disease 2019(COVTD-19)is often accompanied by gastrointestinal symptoms,which are related to gut microbiota dysbiosis(GMD).Whether washed microbiota transplantation(WMT)is an effective treatment ... Objective:Coronavirus disease 2019(COVTD-19)is often accompanied by gastrointestinal symptoms,which are related to gut microbiota dysbiosis(GMD).Whether washed microbiota transplantation(WMT)is an effective treatment for COV1D-19 patients suspected of having GMD by restoring the gut microbiota is unknown.This study is designed to explore the efficacy and safety of WMT in COVID-19 patients suspected of having GMD.Methods:This is a randomized,multicenter,single-blind prospective study.COVID-19 patients suspected of having GMD will be randomly divided to receive routine treatment only or to receive routine treatment and WMT.The frequency of WMT will be once a day for three consecutive days.Laboratory and imaging examinations will be performed at admission,1 and 2 weeks after treatment,and on the day of discharge.Then a telephone follow-up will be conducted at 1st week,2nd week,and 6th month after discharge.The clinical efficacy and safety of WMT in COVD-19 patients suspected of having GMD and the effects of WMT on the organ function,homeostasis,inflammatory response,intestinal mucosal barrier function,and immunity of the patients will be evaluated.Results:By following the proposed protocol,WMT is expected to be efficacious and safe for the treatment of COVID-19 patients suspected of having GMD,and the therapeutic effect is expected to be associated with improvement of the intestinal mucosal barrier function,inflammatory response,and immunity.Conclusion:The findings from this study may offer a new approach for the prevention and treatment of COVID-19 patients suspected of having GMD. 展开更多
关键词 COVID-19 SARS-Cov-2 virus gut microbiota MICROBIOME DYSBIOSIS washed microbiota transplantation
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Fabrication of niobium doped titanate nanoflakes with enhanced visible-light-driven photocatalytic activity for efficient ibuprofen degradation 被引量:4
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作者 Wen Liu Wei Zhang +4 位作者 Mushi Liu Penghui Du Chenyuan Dang Jialiang Liang Yunyi Li 《Chinese Chemical Letters》 SCIE CAS CSCD 2019年第12期2177-2180,共4页
In this study,a novel class of niobium(Nb) doped titanate nanoflakes(TNFs) are fabricated through a onestep hydrothermal method.Nb doping affects the curving of titanate nanosheet,leading to the formation of nanoflake... In this study,a novel class of niobium(Nb) doped titanate nanoflakes(TNFs) are fabricated through a onestep hydrothermal method.Nb doping affects the curving of titanate nanosheet,leading to the formation of nanoflake structure.In addition,Nb5+ filled in the interlayers of [TiO6] alters the light adsorption property of pristine titanate.The band gap of Nb-TNFs is narrowed to 2.85 eV,while neat titanate nano tubes(TNTs) is 3.4 eV.The enhanced visible light adsorption significantly enhances the visible-lightdriven activity of Nb-TNFs for ibuprofen(IBP) degradation.The pseudo-first order kinetics constant for Nb-TNFs is calculated to be 1.04 h^-1,while no obvious removal is observed for TNTs.Photo-generated holes(h^+) and hydroxyl radicals(·OH) are responsible for IBP degradation.The photocatalytic activity of Nb-TNFs depends on pH condition,and the optimal pH value is found to be 5.In addition,Nb-TNFs exhibited superior photo-stability during the reuse cycles.The results demonstrated Nb-TNFs are very promising in photocatalytic water purification. 展开更多
关键词 TITANATE NIOBIUM Photocatalysis Element doping IBUPROFEN
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A flexible architecture for job management in a grid environment 被引量:2
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作者 LUAN Cui-ju SONG Guang-hua +1 位作者 ZHENG Yao ZHANG Ji-fa 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2007年第1期95-105,共11页
Job management is a key issue in computational grids, and normally involves job definition, scheduling, executing and monitoring. However, job management in the existing grid middleware needs to be improved in terms o... Job management is a key issue in computational grids, and normally involves job definition, scheduling, executing and monitoring. However, job management in the existing grid middleware needs to be improved in terms of efficiency and flexibility. This paper addresses a flexible architecture for job management with detailed design and implementation. Frameworks for job scheduling and monitoring, as two important aspects, are also presented. The proposed job management has the advantages of reusability of job definition, flexible and automatic file operation, visual steering of file transfer and job execution, and adaptive application job scheduler. A job management wizard is designed to implement each step. Therefore, what the grid user needs to do is only to define the job by constructing necessary information at runtime. In addition, the job space is adopted to ensure the security of the job management. Experimental results showed that this approach is user-friendly and system efficient. 展开更多
关键词 Grid Job management Job definition reuse Steering of job transfer Job space
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