The rapid and label-free diagnosis of malignancies in ex vivo breast biopsy tissues has significant utility in pathology laboratories and operating rooms.We report a MEMS-based platform integrated with microchips that...The rapid and label-free diagnosis of malignancies in ex vivo breast biopsy tissues has significant utility in pathology laboratories and operating rooms.We report a MEMS-based platform integrated with microchips that performs phenotyping of breast biopsy tissues using electrothermal sensing.The microchip,fabricated on a silicon substrate,incorporates a platinum microheater,interdigitated electrodes(IDEs),and resistance temperature detectors(RTDs)as on-chip sensing elements.The microchips are integrated onto the platform using a slide-fit contact enabling quick replacement for biological measurements.The bulk resistivity(ρB),surface resistivity(ρS),and thermal conductivity(k)of deparaffinized and formalin-fixed paired tumor and adjacent normal breast biopsy samples from N=8 patients were measured.For formalin-fixed samples,the meanρB for tumors showed a statistically significant fold change of 4.42(P=0.014)when the tissue was heated from 25°C to 37°C compared to the adjacent normal tissue,which showed a fold change of 3.47.The meanρS measurements also showed a similar trend.The mean k of the formalinfixed tumor tissues was 0.309±0.02Wm^(-1)K^(-1)compared to a significantly higher k of 0.563±0.028 Wm^(-1)K^(-1)for the adjacent normal tissues.A similar trend was observed inρB,ρS,and k for the deparaffinized tissue samples.An analysis of a combination ofρ_(B,)ρ_(S,)and k using Fisher’s combined probability test and linear regression suggests the advantage of using all three parameters simultaneously for distinguishing tumors from adjacent normal tissues with higher statistical significance.展开更多
基金Anil Vishnu G.K.acknowledges award funding from BIRAC under the SITAREGYTI scheme(BIRAC SRISTI PMU-2020/001)Anil Vishnu G.K.also acknowledges Midhun C.Kachappilly for helping with the experiments.Annapoorni Rangarajan acknowledges DBT-IISc partnership program.Hardik J.Pandya acknowledges the Core Research Grant support(CRG/2019/004963)from the Science and Engineering Research Board(SERB).
文摘The rapid and label-free diagnosis of malignancies in ex vivo breast biopsy tissues has significant utility in pathology laboratories and operating rooms.We report a MEMS-based platform integrated with microchips that performs phenotyping of breast biopsy tissues using electrothermal sensing.The microchip,fabricated on a silicon substrate,incorporates a platinum microheater,interdigitated electrodes(IDEs),and resistance temperature detectors(RTDs)as on-chip sensing elements.The microchips are integrated onto the platform using a slide-fit contact enabling quick replacement for biological measurements.The bulk resistivity(ρB),surface resistivity(ρS),and thermal conductivity(k)of deparaffinized and formalin-fixed paired tumor and adjacent normal breast biopsy samples from N=8 patients were measured.For formalin-fixed samples,the meanρB for tumors showed a statistically significant fold change of 4.42(P=0.014)when the tissue was heated from 25°C to 37°C compared to the adjacent normal tissue,which showed a fold change of 3.47.The meanρS measurements also showed a similar trend.The mean k of the formalinfixed tumor tissues was 0.309±0.02Wm^(-1)K^(-1)compared to a significantly higher k of 0.563±0.028 Wm^(-1)K^(-1)for the adjacent normal tissues.A similar trend was observed inρB,ρS,and k for the deparaffinized tissue samples.An analysis of a combination ofρ_(B,)ρ_(S,)and k using Fisher’s combined probability test and linear regression suggests the advantage of using all three parameters simultaneously for distinguishing tumors from adjacent normal tissues with higher statistical significance.
