Raman spectroscopy is a noninvasive,nondestructive analytical method capable of determining the biochemical constituents based on molecular vibrations.It does not require sample preparation or pretreatment.However,the...Raman spectroscopy is a noninvasive,nondestructive analytical method capable of determining the biochemical constituents based on molecular vibrations.It does not require sample preparation or pretreatment.However,the use of Raman spectroscopy for in vivo clinical applications will depend on the feasibility of measuring Raman spectra in a relatively short time period(a few seconds).In this work,a fast dispersive-type nearinfrared(NIR)Raman spectroscopy system and a skin Raman probe were developed to facilitate real-time,noninvasive,in vivo human skin measurements.Spectrograph image aberration was corrected by a parabolic-line fiber array,permitting complete CCD vertical binning,thereby yielding a 16-fold improvement in signal-to-noise ratio.Good quality in vivo skin NIR Raman spectra free of interference from fiber fluorescence and silica Raman scattering can be acquired within one second,which greatly facilitates practical noninvasive tissue characterization and clinical diagnosis.Currently,we are conducting a large clinical study of various skin diseases in order to develop Raman spectroscopy into a useful tool for non-invasive skin cancer detection.Intermediate data analysis results are presented.Recently,we have also successfully developed a technically more challenging endoscopic Laser-Raman probe for early lung cancer detection.Preliminary in vivo results from endoscopic lung Raman measurements are discussed.展开更多
A novel and compact near-infrared （NIR） Raman system is developed using 785-nm diode laser, volumephase technology （VPT） holographic system, and NIR intensified charge-coupled device （CCD）. Signal-tonoise ratio ...A novel and compact near-infrared （NIR） Raman system is developed using 785-nm diode laser, volumephase technology （VPT） holographic system, and NIR intensified charge-coupled device （CCD）. Signal-tonoise ratio （SNR） and resolution are improved compared with ordinary acquisition method by a specially designed optical fiber detector and the spectrograph image aberration correction with a parabolic-line fiber array. In 1-5 s, Raman spectra of different parts of Chinese human skin are acquired. Autofluorescence is subtracted from the raw spectrum by polynomial fitting and skin Raman spectrum is then smoothed for further analysis.展开更多
基金国家自然科学基金项目(60778046)福建省科技项目(2005I009)the Canadian Institutes of Health Research/National Science Foundation of China International Scientific Exchange Program(60711120031)资助
基金supported by the National Cancer Institute of Canada with funds from the Canadian Cancer Society,the Canadian Institutes of Health Research(Grant No.PPP-79109 and MOP-85011)the Canadian Dermatology Foundation,the VGH&UBC Hospital Foundation In It for Life Fund,and the BC Hydro Employees Community Services Fund.
文摘Raman spectroscopy is a noninvasive,nondestructive analytical method capable of determining the biochemical constituents based on molecular vibrations.It does not require sample preparation or pretreatment.However,the use of Raman spectroscopy for in vivo clinical applications will depend on the feasibility of measuring Raman spectra in a relatively short time period(a few seconds).In this work,a fast dispersive-type nearinfrared(NIR)Raman spectroscopy system and a skin Raman probe were developed to facilitate real-time,noninvasive,in vivo human skin measurements.Spectrograph image aberration was corrected by a parabolic-line fiber array,permitting complete CCD vertical binning,thereby yielding a 16-fold improvement in signal-to-noise ratio.Good quality in vivo skin NIR Raman spectra free of interference from fiber fluorescence and silica Raman scattering can be acquired within one second,which greatly facilitates practical noninvasive tissue characterization and clinical diagnosis.Currently,we are conducting a large clinical study of various skin diseases in order to develop Raman spectroscopy into a useful tool for non-invasive skin cancer detection.Intermediate data analysis results are presented.Recently,we have also successfully developed a technically more challenging endoscopic Laser-Raman probe for early lung cancer detection.Preliminary in vivo results from endoscopic lung Raman measurements are discussed.
基金This work was supported by the Key Project of Ministry of Education (No. 205081)the Project of Fujian Province (No. 20051009, JA04168)the Project of Fujian Development and Reform Commission (No.2005-847).
文摘A novel and compact near-infrared （NIR） Raman system is developed using 785-nm diode laser, volumephase technology （VPT） holographic system, and NIR intensified charge-coupled device （CCD）. Signal-tonoise ratio （SNR） and resolution are improved compared with ordinary acquisition method by a specially designed optical fiber detector and the spectrograph image aberration correction with a parabolic-line fiber array. In 1-5 s, Raman spectra of different parts of Chinese human skin are acquired. Autofluorescence is subtracted from the raw spectrum by polynomial fitting and skin Raman spectrum is then smoothed for further analysis.
