目的评估双源CT Sn 100kVp能谱纯化用于低剂量肺结节筛查的诊断效能。方法纳入2020年4月至2023年12月期间在南京医科大学附二院接受至少两次低剂量CT检查的100名肺结节患者,将初检患者纳入常规电压组[(以110 kVp、52 mAs行常规电压低剂...目的评估双源CT Sn 100kVp能谱纯化用于低剂量肺结节筛查的诊断效能。方法纳入2020年4月至2023年12月期间在南京医科大学附二院接受至少两次低剂量CT检查的100名肺结节患者,将初检患者纳入常规电压组[(以110 kVp、52 mAs行常规电压低剂量螺旋CT(LDCT)扫描)],复检患者纳入低电压组(以Sn 100kVp、70 mAs行低电压能谱纯化低剂量双源CT扫描),比较两组辐射剂量、客观图像质量评价、主观图像质量评分及诊断效能。结果低电压组的容积CT剂量指数(CTDIvol)、剂量长度乘积(DLP)和有效辐射剂量(ED)均显著低于常规电压组(P均<0.05)。低电压组肺窗标准差(SD)低于常规电压组,肺窗对比噪声比(CNR)高于常规电压组(P均<0.05)。低电压组肺窗、纵隔窗主观图像质量评分低于常规电压组(P均<0.05)。与病理结果相比,常规电压LDCT检测肺结节的Kappa指数为0.944,双源CT Sn 100kVp检测肺结节的Kappa指数为0.917,均与病理结果一致性良好。结论本研究表明,双源CT Sn 100kVp能谱纯化技术在低剂量肺结节筛查中展现出良好的诊断效能,同时显著降低了辐射剂量,为临床提供了一种更为安全有效的筛查方法。展开更多
AIM:To determine whether paeonol(Pae),a naturally occurring phenolic compound,can serve as an effective pharmacological inhibitor of posterior capsular opacification(PCO).METHODS:A rat model of cataract surgery—induc...AIM:To determine whether paeonol(Pae),a naturally occurring phenolic compound,can serve as an effective pharmacological inhibitor of posterior capsular opacification(PCO).METHODS:A rat model of cataract surgery—induced PCO was established,and Pae was administered via anterior chamber injection to evaluate its preventive effect on capsular opacification and fibrotic remodeling.Histological and immunohistochemical analyses were performed to assess epithelial-mesenchymal transition(EMT)—related changes in lens epithelial cells(LECs).Ex vivo lens capsule cultures were employed to examine the expression of Vimentin and Zonula Occludens-1(ZO-1)by immunofluorescence and immunohistochemistry.In the human LEC line SRA01/04,EMT marker expression at both mRNA and protein levels was analyzed following transforming growth factor beta 2(TGF-β2)stimulation,with Pae treatment.Western blotting and immunofluorescence were used to investigate the effect of Pae on TGF-β/Smad signaling and AMP-activated protein kinase(AMPK)activation.Molecular docking was performed to predict Pae–AMPK binding,and rescue experiments with AMPK inhibition were conducted to validate the mechanistic pathway.RESULTS:Pae significantly reduced capsular opacification and fibrotic remodeling in the rat PCO model compared with controls.In LECs,Pae markedly suppressed TGF-β2–induced EMT,evidenced by decreased expression of mesenchymal markers,such as Vimentin,Fibronectin,Collagen 1A1,α-SMA and preserved epithelial junctional protein ZO-1.Mechanistically,Pae was predicted to directly interact with the catalytic pocket of AMPK,which was experimentally confirmed by enhanced AMPK phosphorylation and nuclear translocation(P<0.05).This activation disrupted canonical TGF-β/Smad signaling,leading to suppression of EMT.Rescue experiments using AMPK inhibition abrogated the anti-EMT effect of Pae,further validating the AMPK-dependent mechanism.CONCLUSION:Pae exerts a potent inhibitory effect on PCO formation by blocking EMT of LECs through direct activation of AMPK and subsequent disruption of TGF-β/Smad signaling.展开更多
文摘目的评估双源CT Sn 100kVp能谱纯化用于低剂量肺结节筛查的诊断效能。方法纳入2020年4月至2023年12月期间在南京医科大学附二院接受至少两次低剂量CT检查的100名肺结节患者,将初检患者纳入常规电压组[(以110 kVp、52 mAs行常规电压低剂量螺旋CT(LDCT)扫描)],复检患者纳入低电压组(以Sn 100kVp、70 mAs行低电压能谱纯化低剂量双源CT扫描),比较两组辐射剂量、客观图像质量评价、主观图像质量评分及诊断效能。结果低电压组的容积CT剂量指数(CTDIvol)、剂量长度乘积(DLP)和有效辐射剂量(ED)均显著低于常规电压组(P均<0.05)。低电压组肺窗标准差(SD)低于常规电压组,肺窗对比噪声比(CNR)高于常规电压组(P均<0.05)。低电压组肺窗、纵隔窗主观图像质量评分低于常规电压组(P均<0.05)。与病理结果相比,常规电压LDCT检测肺结节的Kappa指数为0.944,双源CT Sn 100kVp检测肺结节的Kappa指数为0.917,均与病理结果一致性良好。结论本研究表明,双源CT Sn 100kVp能谱纯化技术在低剂量肺结节筛查中展现出良好的诊断效能,同时显著降低了辐射剂量,为临床提供了一种更为安全有效的筛查方法。
基金Supported by the Projects of Medical and Health Technology Development Program in Shandong Province(No.202107021009)Shandong Provincial Traditional Chinese Medicine Science and Technology Project(No.M-2023118).
文摘AIM:To determine whether paeonol(Pae),a naturally occurring phenolic compound,can serve as an effective pharmacological inhibitor of posterior capsular opacification(PCO).METHODS:A rat model of cataract surgery—induced PCO was established,and Pae was administered via anterior chamber injection to evaluate its preventive effect on capsular opacification and fibrotic remodeling.Histological and immunohistochemical analyses were performed to assess epithelial-mesenchymal transition(EMT)—related changes in lens epithelial cells(LECs).Ex vivo lens capsule cultures were employed to examine the expression of Vimentin and Zonula Occludens-1(ZO-1)by immunofluorescence and immunohistochemistry.In the human LEC line SRA01/04,EMT marker expression at both mRNA and protein levels was analyzed following transforming growth factor beta 2(TGF-β2)stimulation,with Pae treatment.Western blotting and immunofluorescence were used to investigate the effect of Pae on TGF-β/Smad signaling and AMP-activated protein kinase(AMPK)activation.Molecular docking was performed to predict Pae–AMPK binding,and rescue experiments with AMPK inhibition were conducted to validate the mechanistic pathway.RESULTS:Pae significantly reduced capsular opacification and fibrotic remodeling in the rat PCO model compared with controls.In LECs,Pae markedly suppressed TGF-β2–induced EMT,evidenced by decreased expression of mesenchymal markers,such as Vimentin,Fibronectin,Collagen 1A1,α-SMA and preserved epithelial junctional protein ZO-1.Mechanistically,Pae was predicted to directly interact with the catalytic pocket of AMPK,which was experimentally confirmed by enhanced AMPK phosphorylation and nuclear translocation(P<0.05).This activation disrupted canonical TGF-β/Smad signaling,leading to suppression of EMT.Rescue experiments using AMPK inhibition abrogated the anti-EMT effect of Pae,further validating the AMPK-dependent mechanism.CONCLUSION:Pae exerts a potent inhibitory effect on PCO formation by blocking EMT of LECs through direct activation of AMPK and subsequent disruption of TGF-β/Smad signaling.
