Roads are a leading cause of habitat fragmentation and may reduce bird populations by increasing nest predation rates.However,few studies have investigated the effects of traffic volume on the reproductive success of ...Roads are a leading cause of habitat fragmentation and may reduce bird populations by increasing nest predation rates.However,few studies have investigated the effects of traffic volume on the reproductive success of roadside birds in the neotropics.Our goal was to evaluate the effects of spatial,temporal,and vehicle flow variations on the survival of artificial open-cup nests.The study was carried out in a nature reserve on the side of a highway during the breeding season(from October to March)in two restinga(sand-coastal plain)phytophysiognomies in southeastern Brazil:non-floodable(open)and floodable(closed).One hundred thirty nests were distributed along transects ranging from 3 m to 300 m from the highway in each vegetation type(totaling 260 nests).The nests were checked every three days for their status(depredated or intact)over 12 days,and new nests were subsequently placed near sampling points of depredated or successful nests.We estimated survival using logistic exposure generalized linear and additive mixed models.At the end of the 180 days of the experiment,33%of 6202 nests were successful.Nest survival was higher in open restinga than in closed restinga.In both habitats,nest survival was lowest mid-season and highest at the beginning and end.Survival rates peaked near the highway,declined up to 50 m away,then showed a slight increase.Finally,survival increased at moderate-to-high traffic volumes(∼22,000 vehicles/day),particularly in open restinga.We suggest that spatial,temporal,and habitat-specific highway impacts(e.g.,noise,vibration,visual stimuli)can lead to variations in the activity of nest predators,generating fluctuations in nest survival associated with predator behavior.展开更多
The increasing demand for energy has intensified recently, requiring alternative sources to fossil fuels, which have become economically and environmentally unfeasible. On the other hand, the increasing land occupatio...The increasing demand for energy has intensified recently, requiring alternative sources to fossil fuels, which have become economically and environmentally unfeasible. On the other hand, the increasing land occupation in recent centuries is a growing problem, demanding greater efficiency, particularly in the reuse of abandoned areas, which has become an alternative. An interesting alternative would be installing energy facilities like solar, wind, biomass, and geothermal, in these areas. The objective of this paper is to develop a classification methodology, based on Artificial Intelligence (AI) and Quantum Theory (QT), to automatically carry out the classification of abandoned areas suitable for the settlement of these power plants. Artificial Neural Networks (ANNs) improved by the hybrid algorithm Quantum-behaved Particle Swarm Optimization (QPSO) together with the Levenberg-Marquardt Algorithm (LMA) were used for the classification task. In terms of Mean Squared Error (MSE), the QPSO-LMA approach achieved a decrease of 19.6% in relation to the classical LMA training with random initial weights. Moreover, the model’s accuracy showed an increase of 7.3% for the QPSO-LMA over the LMA. To validate this new approach, it was also tested on six different datasets available in the UCI Machine Learning Repository and seven classical techniques established in the literature. For the problem of installing photovoltaic plants in abandoned areas, the knowledge acquired with the solar dataset can be extrapolated to other regions.展开更多
Background: Previous research has revealed a strong inflammatory response within adipose(AT) tissue during the transition into lactation. Whether this effect is a result of oxidative stress induced by lipolysis and fa...Background: Previous research has revealed a strong inflammatory response within adipose(AT) tissue during the transition into lactation. Whether this effect is a result of oxidative stress induced by lipolysis and fatty acid oxidation associated with differences in prepartum body condition score remains to be determined. The objectives of this study were to investigate systemic biomarkers of energy balance and inflammation and the expression of lipid metabolismand inflammation-related genes in AT during the transition period in dairy cows.Results: Twenty multiparous Holstein cows were retrospectively divided by body condition score(BCS) prior to parturition into two groups(10 cows/group): BCS ≤ 3.25(Lo BCS) and BCS ≥ 3.75(Hi BCS). Subcutaneous adipose tissue was biopsied from the tail-head region at d-10, 7 and 20 relative to parturition. Plasma was used to evaluate biomarkers of energy balance(EBAL) [free fatty acids(NEFA), glycerol, insulin] and inflammation [IL-1β, haptoglobin, myeloperoxidase, and reactive oxygen metabolites(ROM)]. Although insulin concentration was not affected by BCS, NEFA was overal greater and glycerol lower in HiB CS cows. Greater activity of myeloperoxidase in plasma coincided with increased haptoglobin and IL-1β postpartum in LoB CS cows.Among genes related with oxidative stress, the expression of the cytosolic antioxidant enzyme SOD1 was greater in LoB CS compared to HiB CS. Cows in LoB CS compared with HiB CS had greater overal expression of ABDH5 and ATGL along with ADIPOQ, indicating enhanced basal lipolysis and secretion of adiponectin. Expression of CPT1 A, ACADVL, and ACOX1 was greater overal in HiB CS than LoB CS indicating enhanced NEFA oxidation. Although the temporal increase in plasma NEFA regardless of BCS coincided with the profile of CPT1 A, the gradual decrease in genes related with reesterification of NEFA(PCK1) and glycerol efflux(AQP7) coupled with an increase in glycerol kinase(GK) suggested some stimulation of NEFA utilization within adipose tissue. This idea is supported in part by the gradual decrease in insulin regardless of BCS. Although expression of the inflammation-related gene toll-like receptor 4( TLR4) was greater in Hi BCS versus Lo BCS cows at-10 d, expression of TLR9 was greater in Hi BCS versus Lo BCS at 20 d.These profiles did not seem to be associated with concentrations of pro-inflammatory biomarkers or ROM.Conclusions: Overall, data indicated that cows with BCS 3.25 or lower before calving experienced greater alterations in systemic inflammation and basal lipolysis without excessive increases in NEFA plasma concentrations.Despite the greater plasma NEFA around parturition, cows with BCS 3.75 or higher seemed to have a more active system for catabolism of NEFA and utilization of glycerol within adipose tissue. A linkage between those pathways and risk of disorders postpartum remains to be determined.展开更多
In order to exploit native fish species for aquaculture, basic parameters of artificial reproducetion and estimates of reproductive potential for this fish must be determined to improve hatchery success. We analyze se...In order to exploit native fish species for aquaculture, basic parameters of artificial reproducetion and estimates of reproductive potential for this fish must be determined to improve hatchery success. We analyze seven Brazilian commercial fish. Spawning was induced using the hypophysation method, with injection of crude carp pituitary extract (CCPE). Characiformes species signaled the spawning time with behavioral alterations (usually circular movements). The time of oocyte extrusion varied from 5.3 ± 0.3 h in Salminus franciscanus to 8.6 ± 0.1 h in Prochilodus argenteus after the second CCPE injection. The greatest diameter of a vitellogenic oocyte was registered for P. argenteus (873.9 ± 122.2 μm) and the smallest was for Pimelodus maculates (465.4 ± 36.3 μm). The diameter of vitellogenic oocytes was very similar among species belonging to the same family. The highest ROI values were registered for Prochilodus costatus(27.0 ± 3.5 μm) and P. argenteus (23.4 ± 3.7 μm), while the lowest values were found in Pseudoplatystoma corruscans (4.6 ± 0.9 μm) and P. maculatus (6.0 ± 1.3 μm). Absolute fecundity (number of oocytes released at spawning) was lowest in P. maculatus and highest in P. corruscans. Relative fecundity (RF) presented a high correlation (r = 0.98 to 0.99) with gonad weight, indicating high efficiency of spawning. The RF also presented a high correlation with body weight (r = 0.88 to 0.97) and total length (r = 0.86 to 0.92). The highest RF was registered for Leporinus obtusidens, and the lowest was for P. corruscans. Released fecundity had a negative correlation with the diameter of vitellogenic oocytes and with the height of the follicular cells, and a positive correlation with the thickness of the zona pelucida. These results indicate that the species studied herein are suitable candidates for aquaculture due to their reproductive and zootechnical characteristics.展开更多
The protective role of(poly)phenols against metabolic disorders has been extensively studied in adults but not in adolescents.To assess associations of dietary(poly)phenols and their subclasses with cardiometabolic he...The protective role of(poly)phenols against metabolic disorders has been extensively studied in adults but not in adolescents.To assess associations of dietary(poly)phenols and their subclasses with cardiometabolic health parameters in adolescents.A cross-sectional study was conducted in 944 individuals aged 11–14 years enrolled in the SI!Program for Secondary Schools trial(NCT03504059).(Poly)phenol intake was assessed using semiquantitative food frequency questionnaires and the Phenol-Explorer database.The measured cardiometabolic parameters were waist circumference(WC)age-sex Z-score,blood pressure(BP)age-sex Z-score,blood glucose(BG),triglycerides(TG),and high-density lipoprotein cholesterol(HDL-c).Multilevel mixedeffect linear regression models were applied to examine the association between(poly)phenol quintiles and cardiometabolic health parameters.Compared to the lowest quintile,adolescents in the highest quintile of total(poly)phenol intake had lower WC Z-scores,mean arterial pressure Z-scores,and HDL-c after multivariable adjustment.The WC Z-scores and HDL-c were lower in the highest quintile of flavonoid intake compared to the lowest quintile.The highest quintile of phenolic acid intake was associated with a lower WC Z-score and TG levels,and the highest quintile of stilbene intake with lower BG and TG,and with higher HDL-c compared to the lowest quintile.A higher intake of(poly)phenols,especially flavonoids,phenolic acids,and stilbenes,was associated with better cardiometabolic parameters in adolescents.展开更多
The local interpretable model-agnostic explanation method was used to unveil substructures(toxic alerts)that cause endocrine disruption in chemical compounds using machine learning models.The random forest classifier ...The local interpretable model-agnostic explanation method was used to unveil substructures(toxic alerts)that cause endocrine disruption in chemical compounds using machine learning models.The random forest classifier was applied to build explainable models with the TOX21 data sets after data curation.Using these models applied to the EDC and EDKB-FDA data sets,the substructures that cause endocrine disruption in chemical compounds were unveiled,providing stable,more specific,and consistent explanations,which are essential for trust and acceptance of the findings,mainly due to the difficulty of finding relevant experimental evidence for different receptors(androgen,estrogen,aryl hydrocarbon,aromatase,and peroxisome proliferatoractivated receptors).This approach is significant because of its contribution to the interpretability of explainable machine learning algorithms,particularly in the context of unveiling substructures associated with endocrine disruption in five targets(androgen receptor,estrogen receptor,aryl hydrocarbon receptors,aromatase receptors,and peroxisome proliferator-activated receptors),thereby advancing the relevant field of environmental toxicology,where a careful evaluation of the potential risks of exposure to new compounds is needed.The specific substructures thiophosphate,sulfamate,anilide,carbamate,sulfamide,and thiocyanate are presented as toxic alerts that cause endocrine disruption to better understand their potential risks and adverse effects on human health and the environment.展开更多
BACKGROUND The question of whether a colonoscopist should evaluate anal diseases is relevant.Endoscopists need to be aware of the possibility of anal neoplasms during a colonoscopy,as they can be easily overlooked if ...BACKGROUND The question of whether a colonoscopist should evaluate anal diseases is relevant.Endoscopists need to be aware of the possibility of anal neoplasms during a colonoscopy,as they can be easily overlooked if not properly examined.Specifically,one must clarify the responsibility of the colonoscopist in the diagnosis of anal neoplasms.Anal cancer is relatively rare,accounting for less than 2%of all cases annually.Owing to its rarity,population screening for anal cancer is not indicated,and monitoring is limited to high-risk groups.However,the number of anal cancer cases in high-risk groups has increased over the past four decades worldwide.AIM To assess the results of anal examinations performed during routine colonoscopy and emphasize the importance of diagnosing anal neoplasms.METHODS This was a retrospective study of 16836 patients who were screened by colonoscopy and received a detailed anal examination by videoanoscopy between 2006 and 2024.Among several other findings,the presence of anal neoplasms and suspicious anal cancer lesions was observed.All examinations,including complete anal examination,inspection,digital rectal examination,and videoanoscopy,were performed,and images were recorded and reported.The examinations were individually viewed by the work group,and the findings were analyzed.RESULTS Among the 22676 colonoscopies performed,16836 patients were identified,and 88 lesions suspected of neoplasia(0.52%)were found.Among them,there were 23 cases of neoplasia(0.13%),9 cases of confirmed squamous cell carcinoma of the anal canal(0.05%),5 cases of adenocarcinoma in the anal canal(0.03%),3 cases of rare neoplasms(0.01%),and 6 cases of adenoma(0.03%).CONCLUSION The systematic performance of anal examinations and anoscopy during routine colonoscopy allows the identification of numerous anal diseases,including incidental cases of anal cancer.展开更多
Type 2 diabetes mellitus(T2DM) is a complex disease in which both genetic and environmental factors interact in determining impaired β-cell insulin secretion and peripheral insulin resistance. Insulin resistance in m...Type 2 diabetes mellitus(T2DM) is a complex disease in which both genetic and environmental factors interact in determining impaired β-cell insulin secretion and peripheral insulin resistance. Insulin resistance in muscle, liver and fat is a prominent feature of most patients with T2DM and obesity, resulting in a reduced response of these tissues to insulin. Considerable evidence has been accumulated to indicate that heredity is a major determinant of insulin resistance and T2DM. It is believed that, among individuals destined to develop T2DM, hyperinsulinemia is the mechanism by which the pancreatic β-cell initially compensates for deteriorating peripheral insulin sensitivity, thus ensuring normal glucose tolerance. Most of these people will develop T2DM when β-cells fail to compensate. Despite the progress achieved in this field in recent years, the genetic causes of insulin resistance and T2DM remain elusive.Candidate gene association, linkage and genome-wide association studies have highlighted the role of genetic factors in the development of T2DM. Using these strategies, a large number of variants have been identified in many of these genes, most of which may influence both hepatic and peripheral insulin resistance, adipogenesis and β-cell mass and function. Recently, a new gene has been identified by our research group, the HMGA1 gene, whose loss of function can greatly raise the risk of developing T2DM in humans and mice. Functional genetic variants of the HMGA1 gene have been associated with insulin resistance syndromes among white Europeans, Chinese individuals and Americans of Hispanic ancestry. These findings may represent new ways to improve or even prevent T2DM.展开更多
The pharmacological interventions currently available to control type 2 diabetes mellitus(T2DM) show a wide interindividual variability in drug response, emphasizing the importance of a personalized, more effective me...The pharmacological interventions currently available to control type 2 diabetes mellitus(T2DM) show a wide interindividual variability in drug response, emphasizing the importance of a personalized, more effective medical treatment for each individual patient. In this context, a growing interest has emerged in recent years and has focused on pharmacogenetics, a discipline aimed at understanding the variability in patients' drug response, making it possible to predict which drug is best for each patient and at what doses. Recent pharmacological and clinical evidences indicate that genetic polymorphisms(or genetic variations) of certain genes can adversely affect drug response and therapeutic efficacy of oral hypoglycemic agents in patients with T2 DM, through pharmacokinetic- and/or pharmacodynamic-based mechanisms that may reduce the therapeutic effects or increase toxicity. For example, genetic variants in genes encoding enzymes of the cytochrome P-450 superfamily, or proteins of the ATP-sensitive potassium channel on the beta-cell of the pancreas, are responsible for the interindividual variability of drug response to sulfonylureas in patients with T2 DM. Instead, genetic variants in the genes that encode for the organic cation transporters of metformin have been related to changes in both pharmacodynamic and pharmacokinetic responses to metformin in metformin-treated patients. Thus, based on the individual's genotype, the possibility, in these subjects, of a personalized therapy constitutes the main goal of pharmacogenetics, directly leading to the development of the right medicine for the right patient. Undoubtedly, this represents an integral part of the translational medicine network.展开更多
基金supports were provided by the Concessionária Rodovia do Sol/SA(RodoSol)the Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq,grant:45.6446/2014/1)+2 种基金the São Paulo Research Foundation(FAPESP,Process Number#2024/13237-3)PD received a postdoctoral fellowship from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior(CAPES,grant:88887.469218/2019-00)CD is grateful to CNPq(Bolsa de Produtividade em Pesquisa,grant:308997/2023-9).
文摘Roads are a leading cause of habitat fragmentation and may reduce bird populations by increasing nest predation rates.However,few studies have investigated the effects of traffic volume on the reproductive success of roadside birds in the neotropics.Our goal was to evaluate the effects of spatial,temporal,and vehicle flow variations on the survival of artificial open-cup nests.The study was carried out in a nature reserve on the side of a highway during the breeding season(from October to March)in two restinga(sand-coastal plain)phytophysiognomies in southeastern Brazil:non-floodable(open)and floodable(closed).One hundred thirty nests were distributed along transects ranging from 3 m to 300 m from the highway in each vegetation type(totaling 260 nests).The nests were checked every three days for their status(depredated or intact)over 12 days,and new nests were subsequently placed near sampling points of depredated or successful nests.We estimated survival using logistic exposure generalized linear and additive mixed models.At the end of the 180 days of the experiment,33%of 6202 nests were successful.Nest survival was higher in open restinga than in closed restinga.In both habitats,nest survival was lowest mid-season and highest at the beginning and end.Survival rates peaked near the highway,declined up to 50 m away,then showed a slight increase.Finally,survival increased at moderate-to-high traffic volumes(∼22,000 vehicles/day),particularly in open restinga.We suggest that spatial,temporal,and habitat-specific highway impacts(e.g.,noise,vibration,visual stimuli)can lead to variations in the activity of nest predators,generating fluctuations in nest survival associated with predator behavior.
文摘The increasing demand for energy has intensified recently, requiring alternative sources to fossil fuels, which have become economically and environmentally unfeasible. On the other hand, the increasing land occupation in recent centuries is a growing problem, demanding greater efficiency, particularly in the reuse of abandoned areas, which has become an alternative. An interesting alternative would be installing energy facilities like solar, wind, biomass, and geothermal, in these areas. The objective of this paper is to develop a classification methodology, based on Artificial Intelligence (AI) and Quantum Theory (QT), to automatically carry out the classification of abandoned areas suitable for the settlement of these power plants. Artificial Neural Networks (ANNs) improved by the hybrid algorithm Quantum-behaved Particle Swarm Optimization (QPSO) together with the Levenberg-Marquardt Algorithm (LMA) were used for the classification task. In terms of Mean Squared Error (MSE), the QPSO-LMA approach achieved a decrease of 19.6% in relation to the classical LMA training with random initial weights. Moreover, the model’s accuracy showed an increase of 7.3% for the QPSO-LMA over the LMA. To validate this new approach, it was also tested on six different datasets available in the UCI Machine Learning Repository and seven classical techniques established in the literature. For the problem of installing photovoltaic plants in abandoned areas, the knowledge acquired with the solar dataset can be extrapolated to other regions.
文摘Background: Previous research has revealed a strong inflammatory response within adipose(AT) tissue during the transition into lactation. Whether this effect is a result of oxidative stress induced by lipolysis and fatty acid oxidation associated with differences in prepartum body condition score remains to be determined. The objectives of this study were to investigate systemic biomarkers of energy balance and inflammation and the expression of lipid metabolismand inflammation-related genes in AT during the transition period in dairy cows.Results: Twenty multiparous Holstein cows were retrospectively divided by body condition score(BCS) prior to parturition into two groups(10 cows/group): BCS ≤ 3.25(Lo BCS) and BCS ≥ 3.75(Hi BCS). Subcutaneous adipose tissue was biopsied from the tail-head region at d-10, 7 and 20 relative to parturition. Plasma was used to evaluate biomarkers of energy balance(EBAL) [free fatty acids(NEFA), glycerol, insulin] and inflammation [IL-1β, haptoglobin, myeloperoxidase, and reactive oxygen metabolites(ROM)]. Although insulin concentration was not affected by BCS, NEFA was overal greater and glycerol lower in HiB CS cows. Greater activity of myeloperoxidase in plasma coincided with increased haptoglobin and IL-1β postpartum in LoB CS cows.Among genes related with oxidative stress, the expression of the cytosolic antioxidant enzyme SOD1 was greater in LoB CS compared to HiB CS. Cows in LoB CS compared with HiB CS had greater overal expression of ABDH5 and ATGL along with ADIPOQ, indicating enhanced basal lipolysis and secretion of adiponectin. Expression of CPT1 A, ACADVL, and ACOX1 was greater overal in HiB CS than LoB CS indicating enhanced NEFA oxidation. Although the temporal increase in plasma NEFA regardless of BCS coincided with the profile of CPT1 A, the gradual decrease in genes related with reesterification of NEFA(PCK1) and glycerol efflux(AQP7) coupled with an increase in glycerol kinase(GK) suggested some stimulation of NEFA utilization within adipose tissue. This idea is supported in part by the gradual decrease in insulin regardless of BCS. Although expression of the inflammation-related gene toll-like receptor 4( TLR4) was greater in Hi BCS versus Lo BCS cows at-10 d, expression of TLR9 was greater in Hi BCS versus Lo BCS at 20 d.These profiles did not seem to be associated with concentrations of pro-inflammatory biomarkers or ROM.Conclusions: Overall, data indicated that cows with BCS 3.25 or lower before calving experienced greater alterations in systemic inflammation and basal lipolysis without excessive increases in NEFA plasma concentrations.Despite the greater plasma NEFA around parturition, cows with BCS 3.75 or higher seemed to have a more active system for catabolism of NEFA and utilization of glycerol within adipose tissue. A linkage between those pathways and risk of disorders postpartum remains to be determined.
基金This study was conducted with funds granted by CNPq,FAPEMIG and CODEVASF.
文摘In order to exploit native fish species for aquaculture, basic parameters of artificial reproducetion and estimates of reproductive potential for this fish must be determined to improve hatchery success. We analyze seven Brazilian commercial fish. Spawning was induced using the hypophysation method, with injection of crude carp pituitary extract (CCPE). Characiformes species signaled the spawning time with behavioral alterations (usually circular movements). The time of oocyte extrusion varied from 5.3 ± 0.3 h in Salminus franciscanus to 8.6 ± 0.1 h in Prochilodus argenteus after the second CCPE injection. The greatest diameter of a vitellogenic oocyte was registered for P. argenteus (873.9 ± 122.2 μm) and the smallest was for Pimelodus maculates (465.4 ± 36.3 μm). The diameter of vitellogenic oocytes was very similar among species belonging to the same family. The highest ROI values were registered for Prochilodus costatus(27.0 ± 3.5 μm) and P. argenteus (23.4 ± 3.7 μm), while the lowest values were found in Pseudoplatystoma corruscans (4.6 ± 0.9 μm) and P. maculatus (6.0 ± 1.3 μm). Absolute fecundity (number of oocytes released at spawning) was lowest in P. maculatus and highest in P. corruscans. Relative fecundity (RF) presented a high correlation (r = 0.98 to 0.99) with gonad weight, indicating high efficiency of spawning. The RF also presented a high correlation with body weight (r = 0.88 to 0.97) and total length (r = 0.86 to 0.92). The highest RF was registered for Leporinus obtusidens, and the lowest was for P. corruscans. Released fecundity had a negative correlation with the diameter of vitellogenic oocytes and with the height of the follicular cells, and a positive correlation with the thickness of the zona pelucida. These results indicate that the species studied herein are suitable candidates for aquaculture due to their reproductive and zootechnical characteristics.
基金supported by the SHE Foundation,“la Caixa”Foundation(LCF/PR/CE16/10700001)the Fundacióla Maratóde TV3(grant number 369/C/2016)and by the funding from Idilia Foods(FBG 311240)+6 种基金Support was also provided by the Ministerio de Ciencia,Innovación y Universidades(PID2020-114022RB-I00)CIBEROBN from the Instituto de Salud Carlos III,ISCIII from the Ministerio de Ciencia,Innovación y Universidades,(AEI/FEDER,UE)Generalitat de Catalunya.J.Martínez-Gómez is a postgraduate fellow of the Ministerio de Ciencia e Innovación of Spain at the Residencia de Estudiantes(2020–ongoing)R.F-J is a recipient of grant PI19/01704 funded by the Fondo de Investigación Sanitaria-Instituto de Salud Carlos III(ISCIII)co-funded by the European Regional Development Fund/European Social Fund“A way to make Europe”/“Investing in your future”.The CNIC is supported by the ISCIII,the Ministerio de Ciencia e Innovación(MCIN)and the Pro CNIC Foundation,and is a Severo Ochoa Center of Excellence(CEX2020-001041-S funded by MICIN/AEI/10.13039/501100011033)G.Santos-Beneit is the recipient of grant LCF/PR/MS19/12220001 funded by“la Caixa”Foundation(ID 100010434)A.Tresserra-Rimbau is a Serra Húnter Fellow.E.P.Laveriano-Santos is a FI-SDUR(EMC/503/2021)fellow from the Generalitat de Catalunya.
文摘The protective role of(poly)phenols against metabolic disorders has been extensively studied in adults but not in adolescents.To assess associations of dietary(poly)phenols and their subclasses with cardiometabolic health parameters in adolescents.A cross-sectional study was conducted in 944 individuals aged 11–14 years enrolled in the SI!Program for Secondary Schools trial(NCT03504059).(Poly)phenol intake was assessed using semiquantitative food frequency questionnaires and the Phenol-Explorer database.The measured cardiometabolic parameters were waist circumference(WC)age-sex Z-score,blood pressure(BP)age-sex Z-score,blood glucose(BG),triglycerides(TG),and high-density lipoprotein cholesterol(HDL-c).Multilevel mixedeffect linear regression models were applied to examine the association between(poly)phenol quintiles and cardiometabolic health parameters.Compared to the lowest quintile,adolescents in the highest quintile of total(poly)phenol intake had lower WC Z-scores,mean arterial pressure Z-scores,and HDL-c after multivariable adjustment.The WC Z-scores and HDL-c were lower in the highest quintile of flavonoid intake compared to the lowest quintile.The highest quintile of phenolic acid intake was associated with a lower WC Z-score and TG levels,and the highest quintile of stilbene intake with lower BG and TG,and with higher HDL-c compared to the lowest quintile.A higher intake of(poly)phenols,especially flavonoids,phenolic acids,and stilbenes,was associated with better cardiometabolic parameters in adolescents.
基金CNPq(Grants 573560/2008-0,465259/2014-6 and 302554/2017-3)CAPES(Finance Code 001)+5 种基金FAPESP(Grant 2014/50983-3)the FAPERJ NanoHealth Research Network(E-26/010.000983/2019)The FAPERJ Support Program for Thematic Projects in the State of Rio de Janeiro(210.104/2020)the National Institute of Science and Technology Complex Fluids(INCT-FCx)for fundingASP was recently awarded the Scientist of Our State by FAPERJ(201.186/2022)the research productivity fellowship granted by CNPq(310166/2020-9 and 305839/2023-3).
文摘The local interpretable model-agnostic explanation method was used to unveil substructures(toxic alerts)that cause endocrine disruption in chemical compounds using machine learning models.The random forest classifier was applied to build explainable models with the TOX21 data sets after data curation.Using these models applied to the EDC and EDKB-FDA data sets,the substructures that cause endocrine disruption in chemical compounds were unveiled,providing stable,more specific,and consistent explanations,which are essential for trust and acceptance of the findings,mainly due to the difficulty of finding relevant experimental evidence for different receptors(androgen,estrogen,aryl hydrocarbon,aromatase,and peroxisome proliferatoractivated receptors).This approach is significant because of its contribution to the interpretability of explainable machine learning algorithms,particularly in the context of unveiling substructures associated with endocrine disruption in five targets(androgen receptor,estrogen receptor,aryl hydrocarbon receptors,aromatase receptors,and peroxisome proliferator-activated receptors),thereby advancing the relevant field of environmental toxicology,where a careful evaluation of the potential risks of exposure to new compounds is needed.The specific substructures thiophosphate,sulfamate,anilide,carbamate,sulfamide,and thiocyanate are presented as toxic alerts that cause endocrine disruption to better understand their potential risks and adverse effects on human health and the environment.
文摘BACKGROUND The question of whether a colonoscopist should evaluate anal diseases is relevant.Endoscopists need to be aware of the possibility of anal neoplasms during a colonoscopy,as they can be easily overlooked if not properly examined.Specifically,one must clarify the responsibility of the colonoscopist in the diagnosis of anal neoplasms.Anal cancer is relatively rare,accounting for less than 2%of all cases annually.Owing to its rarity,population screening for anal cancer is not indicated,and monitoring is limited to high-risk groups.However,the number of anal cancer cases in high-risk groups has increased over the past four decades worldwide.AIM To assess the results of anal examinations performed during routine colonoscopy and emphasize the importance of diagnosing anal neoplasms.METHODS This was a retrospective study of 16836 patients who were screened by colonoscopy and received a detailed anal examination by videoanoscopy between 2006 and 2024.Among several other findings,the presence of anal neoplasms and suspicious anal cancer lesions was observed.All examinations,including complete anal examination,inspection,digital rectal examination,and videoanoscopy,were performed,and images were recorded and reported.The examinations were individually viewed by the work group,and the findings were analyzed.RESULTS Among the 22676 colonoscopies performed,16836 patients were identified,and 88 lesions suspected of neoplasia(0.52%)were found.Among them,there were 23 cases of neoplasia(0.13%),9 cases of confirmed squamous cell carcinoma of the anal canal(0.05%),5 cases of adenocarcinoma in the anal canal(0.03%),3 cases of rare neoplasms(0.01%),and 6 cases of adenoma(0.03%).CONCLUSION The systematic performance of anal examinations and anoscopy during routine colonoscopy allows the identification of numerous anal diseases,including incidental cases of anal cancer.
文摘Type 2 diabetes mellitus(T2DM) is a complex disease in which both genetic and environmental factors interact in determining impaired β-cell insulin secretion and peripheral insulin resistance. Insulin resistance in muscle, liver and fat is a prominent feature of most patients with T2DM and obesity, resulting in a reduced response of these tissues to insulin. Considerable evidence has been accumulated to indicate that heredity is a major determinant of insulin resistance and T2DM. It is believed that, among individuals destined to develop T2DM, hyperinsulinemia is the mechanism by which the pancreatic β-cell initially compensates for deteriorating peripheral insulin sensitivity, thus ensuring normal glucose tolerance. Most of these people will develop T2DM when β-cells fail to compensate. Despite the progress achieved in this field in recent years, the genetic causes of insulin resistance and T2DM remain elusive.Candidate gene association, linkage and genome-wide association studies have highlighted the role of genetic factors in the development of T2DM. Using these strategies, a large number of variants have been identified in many of these genes, most of which may influence both hepatic and peripheral insulin resistance, adipogenesis and β-cell mass and function. Recently, a new gene has been identified by our research group, the HMGA1 gene, whose loss of function can greatly raise the risk of developing T2DM in humans and mice. Functional genetic variants of the HMGA1 gene have been associated with insulin resistance syndromes among white Europeans, Chinese individuals and Americans of Hispanic ancestry. These findings may represent new ways to improve or even prevent T2DM.
文摘The pharmacological interventions currently available to control type 2 diabetes mellitus(T2DM) show a wide interindividual variability in drug response, emphasizing the importance of a personalized, more effective medical treatment for each individual patient. In this context, a growing interest has emerged in recent years and has focused on pharmacogenetics, a discipline aimed at understanding the variability in patients' drug response, making it possible to predict which drug is best for each patient and at what doses. Recent pharmacological and clinical evidences indicate that genetic polymorphisms(or genetic variations) of certain genes can adversely affect drug response and therapeutic efficacy of oral hypoglycemic agents in patients with T2 DM, through pharmacokinetic- and/or pharmacodynamic-based mechanisms that may reduce the therapeutic effects or increase toxicity. For example, genetic variants in genes encoding enzymes of the cytochrome P-450 superfamily, or proteins of the ATP-sensitive potassium channel on the beta-cell of the pancreas, are responsible for the interindividual variability of drug response to sulfonylureas in patients with T2 DM. Instead, genetic variants in the genes that encode for the organic cation transporters of metformin have been related to changes in both pharmacodynamic and pharmacokinetic responses to metformin in metformin-treated patients. Thus, based on the individual's genotype, the possibility, in these subjects, of a personalized therapy constitutes the main goal of pharmacogenetics, directly leading to the development of the right medicine for the right patient. Undoubtedly, this represents an integral part of the translational medicine network.
