Metaplastic carcinomas of the breast are rare heterogenous neoplasms characterized by adenocarcinoma with dominant areas of spindle cells, squamous and/or other mesenchymal differentiation, that comprise of <5% of ...Metaplastic carcinomas of the breast are rare heterogenous neoplasms characterized by adenocarcinoma with dominant areas of spindle cells, squamous and/or other mesenchymal differentiation, that comprise of <5% of all invasive breast cancers. Our objective in this study was to review the pathological features and clinical outcomes for metaplastic carcinoma of breast in breast cancer patients registered in CENAR (Center for Nuclear Medicines and Radiotherapy), Balochistan. Present study was performed on 7 patients affected with metaplastic carcinoma of breast, who were registered patients in CENAR. Informed consent was taken from the patients and BMI was calculated by measuring the height and weight of the patients. Available clinical history obtained by retrieving the patients file and a copy of biopsy report was also obtained from the file. Metaplastic carcinoma of breast was 4.11% of all 170 breast cancer cases registered in CENAR from 2010-2012. Mean age was 40 years ranging from 25 - 50 years. Four subtypes of metaplastic carcinoma of breast were reported in this study;DCIS component was present in one case and mean tumor size was 6.12 cm ranging from 3.5 - 10 cm. Metaplastic carcinomas of breast are rare heterogenous neoplasm with different characteristics, demographics and tumor biology and accounts for almost >5% of all breast cancer cases.展开更多
Background:Drug resistance is increasing in gastrointestinal parasitosis;it is therefore essential to identify new anthelmintic molecules.Objective:The study aims to evaluate the anthelmintic activity of extracts and ...Background:Drug resistance is increasing in gastrointestinal parasitosis;it is therefore essential to identify new anthelmintic molecules.Objective:The study aims to evaluate the anthelmintic activity of extracts and fractions of leaves of Saba sene-galensis(A.DC.)Pichon(Apocynaceae)against L_(3)larvae of Caenorhabditis elegans,a free-living helminth model and to highlight potential anthelmintic molecules by dereplication strategy of molecular networking.Methods:The methanolic extract,ethanolic extract and aqueous extract of the plant’s leaves were carried out,followed by flash chromatography on silica gel for by fractionation,HPLC-MS/MS analysis.The tandem mass data were used for generation of molecular network.The anthelmintic effects of aqueous and hydro-ethanolic fractions of leaves of S.senegalensis on Caenorhabditis elegans worm viability were carried out by using both 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide(MTT)assay and microscope motility observation.Results:Among the aqueous fractions,the aqueous decoction showed an EC_(50)at 0.24 mg/ml and a viability rate of 7.5%at 0.9 mg/ml.In hydro-ethanolic extracts,hydro-ethanolic macerate residual(Fe-3)exhibited a significant effect with an EC_(50)at 0.29 mg/ml and 12.5%of viability at 0.9 mg/ml.HPLC-ESI-MS/MS analysis allowed the identification of twelve compounds.Conclusions:Using the MTT-formazan assay,extracts of S.senegalensis exhibit a moderate anthelmintic effect on C.elegans worms linked to natural antiparasitic molecules.展开更多
Lignosus rhinocerus is a highly valued medicinal mushroom traditionally consumed by the indigenous communities of Southeast Asia as treatment for numerous ailments,some of which are linked to its antioxidative activit...Lignosus rhinocerus is a highly valued medicinal mushroom traditionally consumed by the indigenous communities of Southeast Asia as treatment for numerous ailments,some of which are linked to its antioxidative activities.While the antioxidative capacity of L.rhinocerus has been established in vitro,lack of translational studies are available on the effect of L.rhinocerus in the pathogenesis of diseases driven by high oxidative stress.This study aims to investigate the in vivo antioxidative effects of cultivated Lignosus rhinocerus(TM02®)sclerotia in a multifaceted in vivo model of oxidative stress,induced by chronic hyperglycemia associated with diabetes.A type 2 diabetes mellitus(T2DM)rodent model was generated using a high-fat diet in combination with low dose streptozotocin after which TM02®cultivar,at a dose of 200 mg/kg,was administered orally for 14 days.Diabetic rats treated with TM02®cultivar had decreased plasma AGEs concentration,complemented with improved mitochondrial respiratory function associated with elevated cardiac and skeletal muscle ATP concentrations in both diabetic and non diabetic rats.Interestingly,TM02®cultivar-treated rats have improved glycemic control,with the potential ability to induce insulin secretion.Our findings revealed that TM02®exhibits antioxidative effects to potentially alleviate diabetes-related complications associated with AGEs accumulation,with significant effects in enhancing mitochondrial function and energy production.展开更多
文摘Metaplastic carcinomas of the breast are rare heterogenous neoplasms characterized by adenocarcinoma with dominant areas of spindle cells, squamous and/or other mesenchymal differentiation, that comprise of <5% of all invasive breast cancers. Our objective in this study was to review the pathological features and clinical outcomes for metaplastic carcinoma of breast in breast cancer patients registered in CENAR (Center for Nuclear Medicines and Radiotherapy), Balochistan. Present study was performed on 7 patients affected with metaplastic carcinoma of breast, who were registered patients in CENAR. Informed consent was taken from the patients and BMI was calculated by measuring the height and weight of the patients. Available clinical history obtained by retrieving the patients file and a copy of biopsy report was also obtained from the file. Metaplastic carcinoma of breast was 4.11% of all 170 breast cancer cases registered in CENAR from 2010-2012. Mean age was 40 years ranging from 25 - 50 years. Four subtypes of metaplastic carcinoma of breast were reported in this study;DCIS component was present in one case and mean tumor size was 6.12 cm ranging from 3.5 - 10 cm. Metaplastic carcinomas of breast are rare heterogenous neoplasm with different characteristics, demographics and tumor biology and accounts for almost >5% of all breast cancer cases.
基金financial support from Campus France(895739H)through the French Embassy in Burkina Faso.
文摘Background:Drug resistance is increasing in gastrointestinal parasitosis;it is therefore essential to identify new anthelmintic molecules.Objective:The study aims to evaluate the anthelmintic activity of extracts and fractions of leaves of Saba sene-galensis(A.DC.)Pichon(Apocynaceae)against L_(3)larvae of Caenorhabditis elegans,a free-living helminth model and to highlight potential anthelmintic molecules by dereplication strategy of molecular networking.Methods:The methanolic extract,ethanolic extract and aqueous extract of the plant’s leaves were carried out,followed by flash chromatography on silica gel for by fractionation,HPLC-MS/MS analysis.The tandem mass data were used for generation of molecular network.The anthelmintic effects of aqueous and hydro-ethanolic fractions of leaves of S.senegalensis on Caenorhabditis elegans worm viability were carried out by using both 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide(MTT)assay and microscope motility observation.Results:Among the aqueous fractions,the aqueous decoction showed an EC_(50)at 0.24 mg/ml and a viability rate of 7.5%at 0.9 mg/ml.In hydro-ethanolic extracts,hydro-ethanolic macerate residual(Fe-3)exhibited a significant effect with an EC_(50)at 0.29 mg/ml and 12.5%of viability at 0.9 mg/ml.HPLC-ESI-MS/MS analysis allowed the identification of twelve compounds.Conclusions:Using the MTT-formazan assay,extracts of S.senegalensis exhibit a moderate anthelmintic effect on C.elegans worms linked to natural antiparasitic molecules.
基金acknowledge(1)Ministry of Higher Education(MOHE),Malaysia[FRGS/1/2020/STG02/UM/02/1,Fundamental Research Grant Scheme(FRGS)]2)Universiti Malaya for the UMRG grant RP034 B,C-17AFR and(3)postgraduate scholarship granted to Chee-Sum A.Yap by LiGNO Research Initiative,LiGNO Biotech Sdn Bhd.
文摘Lignosus rhinocerus is a highly valued medicinal mushroom traditionally consumed by the indigenous communities of Southeast Asia as treatment for numerous ailments,some of which are linked to its antioxidative activities.While the antioxidative capacity of L.rhinocerus has been established in vitro,lack of translational studies are available on the effect of L.rhinocerus in the pathogenesis of diseases driven by high oxidative stress.This study aims to investigate the in vivo antioxidative effects of cultivated Lignosus rhinocerus(TM02®)sclerotia in a multifaceted in vivo model of oxidative stress,induced by chronic hyperglycemia associated with diabetes.A type 2 diabetes mellitus(T2DM)rodent model was generated using a high-fat diet in combination with low dose streptozotocin after which TM02®cultivar,at a dose of 200 mg/kg,was administered orally for 14 days.Diabetic rats treated with TM02®cultivar had decreased plasma AGEs concentration,complemented with improved mitochondrial respiratory function associated with elevated cardiac and skeletal muscle ATP concentrations in both diabetic and non diabetic rats.Interestingly,TM02®cultivar-treated rats have improved glycemic control,with the potential ability to induce insulin secretion.Our findings revealed that TM02®exhibits antioxidative effects to potentially alleviate diabetes-related complications associated with AGEs accumulation,with significant effects in enhancing mitochondrial function and energy production.
