Human mesenchymal stem cells(hMSCs)were labeled with Dotarem or(Gd-DOTA)2-EM7(EM7Gd2)via electroporation(EP).Cellular transmission electron microscopy(TEM)reveals free distribution of Gd agents and formation of EM7Gd2...Human mesenchymal stem cells(hMSCs)were labeled with Dotarem or(Gd-DOTA)2-EM7(EM7Gd2)via electroporation(EP).Cellular transmission electron microscopy(TEM)reveals free distribution of Gd agents and formation of EM7Gd2 clusters in the cytosol.Cellular magnetic resonance imaging(MRI)reveals that the free Gd agents induce MRI signal enhancement effect due to its fast exocytosis and subsequent interaction with intercellular water molecules.The EM7Gd2 clusters exhibits a longer intracellular retention time and induce a persistent MRI signal reduction effect.The cellular MRI results are interpreted by taking into account both T1 and T2 relaxation rates and their correlation with cellular binding structures of Dotarem and EM7Gd2.展开更多
Aflatoxin B1(AFB1)is one of the most common mycotoxins that threatens human health.As singlestranded oligonucleotides with high affinity and specificity,aptamers have incomparable effect on the targeted detection of A...Aflatoxin B1(AFB1)is one of the most common mycotoxins that threatens human health.As singlestranded oligonucleotides with high affinity and specificity,aptamers have incomparable effect on the targeted detection of AFB1.Herein,after 11 rounds of selection and analysis using a modified affinity chromatography-based SELEX strategy,the truncated 37 nt aptamer AF11-2 was successfully obtained.The aptamer shows good detection performance for AFB1,and can sensitively detect AFB1 in the range of 100-1000 nmol/L,with a detection limit of 42 nmol/L.In the detection of pretreated edible peanut oil samples,AF11-2 aptamer also showed a high recovery rate and good stability for AFB1,and achieved satisfactory results.In addition,AF11-2 aptamer can significantly enhance the fluorescence ability of AFB1,which is not available in traditional Afla17-2-3 aptamer.After molecular docking analysis,it was found that AF11-2 and Afla17-2-3 had different nucleotide binding sites for AFB1.Afla17-2-3 binds to the carbonyl O of AFB1,while AF11-2 binds to the pyrrolic O of AFB1,which may be the main reason that AF11-2 can enhance the fluorescence of AFB1.展开更多
Electrochemical CO_(2)reduction(ECR)is one of the most effective methods to obtain carbonaceous chemicals and reduce greenhouse gases passingly under the ambient condition.However,efficient electrocatalysts featured w...Electrochemical CO_(2)reduction(ECR)is one of the most effective methods to obtain carbonaceous chemicals and reduce greenhouse gases passingly under the ambient condition.However,efficient electrocatalysts featured with high selectivity and stability are still lacking.A novel molecule-mediated Ag electrocatalyst with capped thiols is rationally designed for high-performance ECR.The thiol-capped and carbon-supported Ag nanostructures(Ag-TC)are formed by in situ electrochemical reduction from three-dimentional(3D)Ag-thiol metal-organic compound with cysteine as the anchor agent and carbon source.Ag-TC exhibits high selectivity and stability for CO_(2)conversion to CO(86.7%),which is more catalytically active than that of common Ag nanoparticles.The function of thiols for ECR is proved by replacing cysteine with alanine without thiol group.Meanwhile,alternatively replacing and removing the surface molecules on the Ag foil further demonstrate the effct of thiols.This work enlightens the promise of in situ construction method for molecule capped metal electrocatalyst towards selective and stable ECR.展开更多
Erratum to Nano Research 2022,15(4):3283−3289 https://doi.org/10.1007/s12274-021-3978-7 One funding number in the Acknowledgements section was unfortunately mistakenly used.This error did not affect any of the conclus...Erratum to Nano Research 2022,15(4):3283−3289 https://doi.org/10.1007/s12274-021-3978-7 One funding number in the Acknowledgements section was unfortunately mistakenly used.This error did not affect any of the conclusions from the published paper.展开更多
Mesenchymal stem cells(MSCs)have emerged as promising candidates for idiopathic pulmonary fibrosis(IPF)therapy.Increasing the MSC survival rate and deepening the understanding of the behavior of transplanted MSCs are ...Mesenchymal stem cells(MSCs)have emerged as promising candidates for idiopathic pulmonary fibrosis(IPF)therapy.Increasing the MSC survival rate and deepening the understanding of the behavior of transplanted MSCs are of great significance for improving the efficacy of MSC-based IPF treatment.Therefore,dual-functional Au-based nanoparticles(Au@PEG@PEI@TAT NPs,AuPPT)were fabricated by sequential modification of cationic polymer polyetherimide(PEI),polyethylene glycol(PEG),and transactivator of transcription(TAT)penetration peptide on AuNPs,to co-deliver retinoic acid(RA)and microRNA(miRNA)for simultaneously enhancing MSC survive and real-time imaging tracking of MSCs during IPF treatment.AuPPT NPs,with good drug loading and cellular uptake abilities,could efficiently deliver miRNA and RA to protect MSCs from reactive oxygen species and reduce their expression of apoptosis executive protein Caspase 3,thus prolonging the survival time of MSC after transplantation.In themeantime,the intracellular accumulation of AuPPT NPs enhanced the computed tomography imaging contrast of transplantedMSCs,allowing them to be visually tracked in vivo.This study establishes an Au-based dual-functional platform for drug delivery and cell imaging tracking,which provides a new strategy for MSC-related IPF therapy.展开更多
Acute myeloid leukemia(AML)remains a significant concern in modern medicine.Early diagnosis is the key to improving the therapeutic effects of AML.In the present work,a cascade-targeted and activatable NIR-Ⅱ nanoprob...Acute myeloid leukemia(AML)remains a significant concern in modern medicine.Early diagnosis is the key to improving the therapeutic effects of AML.In the present work,a cascade-targeted and activatable NIR-Ⅱ nanoprobe(Ald&A1094@Ag_(2)S)was developed for early detection of AML in an orthotopic model.Upon intravenous injection,Ald&A1094@Ag_(2)S effectively accumulated in bone tissue due to its high affinity for alendronate(Ald)to the bone.Thereafter,the AML microenvironment allowed for the membrane-penetrating peptide TAT(cell‐penetrating peptide(CGRRRQRRKKRG))to be exposed via pH-sensitive hydrazone bond-mediated detaching of bone-targeted ligands,resulting in efficient internalization of nanoprobes in HL60 cells.Endogenous peroxynitrite(ONOO–)in HL60 cells further activated NIR-Ⅱ fluorescence of Ag_(2)S QDs via A1094 oxidation,thereby inhibiting fluorescence resonance energy transfer(FRET).Such a unique cascade-targeted and activatable strategy enables the nanoprobes to only light up the AML lesion region in the bone marrow with negligible background effects,which holds great potential for clinical applications in the future.展开更多
The second near-infrared(NIR-II,1000-1700 nm)circularly polarized light holds significant untapped potential in areas such as optical anti-counterfeiting and information encryption due to its deeply covert nature.Howe...The second near-infrared(NIR-II,1000-1700 nm)circularly polarized light holds significant untapped potential in areas such as optical anti-counterfeiting and information encryption due to its deeply covert nature.However,the typically low luminescence dissymmetry factor(glum)of circularly polarized luminescence(CPL)materials,particularly in NIR CPL materials,limits their practical application.Addressing this challenge,it is crucial to develop NIR CPL materials with enhanced glum.In this study,we present a series of chiral photonic micro-particles(CPMPs)with tunable chiral photonic bandgaps in the NIR-II range,capable of modulating NIR-II luminescent quantum dots to produce NIR CPL.These CPMPs not only impart chirality to the quantum dots,but also act as carriers,minimizing luminescence quenching from external environments.The tunable chiral photonic bandgap in CPMPs enables the generation of NIR CPL with a high|glum|value of up to 0.81,facilitating the advanced application in covert optical anti-counterfeiting.This work offers a straightforward and viable strategy for the development of NIR CPL materials,broadening their use in invisible information encryption and optical anti-counterfeiting technologies.展开更多
Cation exchange(CE)has been emerged as a promising post-synthesis strategy of colloidal nanocrystals.However,it is unclear how the cation precursor affects the CE process and the final colloidal nanocrystals.Herein,we...Cation exchange(CE)has been emerged as a promising post-synthesis strategy of colloidal nanocrystals.However,it is unclear how the cation precursor affects the CE process and the final colloidal nanocrystals.Herein,we utilized two Zn-B Lewis acidbase adduct complexes(B=oleylamine(OAM)and methanol(MeOH))as Zn precursors for CE with Ag_(2)S quantum dots(QDs).Our study revealed that the steric hindrance and complexing capabilities of Zn precursor significantly affect the CE kinetics.As a result,the Zn-doped Ag_(2)S(Zn:Ag_(2)S)and Ag_(2)S@ZnS core–shell QDs were successfully obtained with enormous enhancement of their photoluminescence(PL)intensities.Theoretical simulation showed that the Zn-OAM with higher desolvation energy and spatial hindrance tended to form doped Zn:Ag_(2)S QDs due to the inefficient cation exchange.Whereas the Zn-MeOH with lower exchange barrier promoted the conversion of Ag-S to Zn-S,thus forming Ag_(2)S@ZnS core–shell QDs.We anticipate that this finding will enrich the regulatory approaches of post-synthesis of colloidal nanocrystals with desirable properties.展开更多
Significant chiroptical responses could be generated by chiral coupling of achiral plasmonic nanoparticles,or originated from intrinsically chiral plasmonic nanoparticles.Here we create dimeric plasmonic metamolecules...Significant chiroptical responses could be generated by chiral coupling of achiral plasmonic nanoparticles,or originated from intrinsically chiral plasmonic nanoparticles.Here we create dimeric plasmonic metamolecules possessing both chiral coupling between nanoparticles and intrinsic chiroptical responses derived from nanoparticles themselves.These plasmonic metamolecules are prepared by assembling helical plasmonic nanorods(HPNRs)with intrinsic chirality in chiral manners on DNA origami template.Two HPNRs with the same or opposite chirality,or one HPNR and one achiral gold nanorod,are coupled chirally into dimeric metamolecules with intriguing plasmonic circular dichroism(PCD).We found that both of the intrinsic chirality of constituent HPNRs and the chiral coupling contribute to the overall PCD while their weights are different in different metamolecules and vary in different wavelength range for a certain metamolecule.Comparing to conventional chiral plasmonic metamolecules from achiral nanoparticles,or discrete chiral nanoparticles,these metamolecules bring more dimensions for tailoring chiroptical responses and make it more flexible to design plasmonic nanodevices with custom PCD.展开更多
Surface ligands of colloidal quantum dots(QDs)have a profound influence on their surface states,which has been verified in the studies of the effect of ligand head groups on the photoluminescence(PL)properties of QDs....Surface ligands of colloidal quantum dots(QDs)have a profound influence on their surface states,which has been verified in the studies of the effect of ligand head groups on the photoluminescence(PL)properties of QDs.However,the investigation of the ligand chain length is limited.Here,we systematically explored the effect of chain length on the Ag_(2)Se QDs by selecting three ligands,1-octanethiol(OTT),1-dodecanethiol(DDT),and 1-hexadecanethiol(HDT),with diverse chain lengths.We found that the PL intensity of Ag_(2)Se QDs increased with the decrease of the ligand chain length due to the enhanced passivation of surface defects emerging from the robust QD-ligand interface binding affinity and the weaker hydrophobic chain–chain interaction.Subsequently,AgAuSe QDs terminated with OTT were obtained by alloying parent OTT-Ag_(2)Se QDs with Au precursor with a record absolute PL quantum yield(PLQY)of 87.2%at 970 nm,facilitating ultrasensitive in vivo angiography imaging in a nude mouse model.We expect that our finding of the important role of the ligand chain length on the optical properties of QDs will be suggestive to the design and synthesis of high-quality QDs,and also look forward to the clinical applications of the ultra-bright AgAuSe QDs.展开更多
The plasma membrane of cells is a crucial biological membrane that involved in a variety of cellular processes including cell signaling transduction through membrane electrical activity.Recently,monitoring membrane el...The plasma membrane of cells is a crucial biological membrane that involved in a variety of cellular processes including cell signaling transduction through membrane electrical activity.Recently,monitoring membrane electrical activity using fluorescence imaging has attracted numerous attentions for its potential applications in evaluating how the nervous system works.However,the development of ideal fluorescent voltage-sensitive probes with both high membrane labeling efficiency and voltage sensitivity is still retain a big challenge.Herein,glutathionecapped CdSe@ZnS quantum dots (CdSe@ZnS-GSH QDs) with a size of 2.5 nm and an emission peak at 520 nm are synthesized using a facile ligand exchange method for plasma membrane labeling and membrane potential imaging.The as-synthesized CdSe@ZnS-GSH QDs can effectively label cell membrane at neutral pH within 30 min and exhibit excellent optical stability in continuous imaging for up to 60 min.With the test concentration up to 200 nM,CdSe@ZnS-GSH QDs show high biocompatibility to cells and do not affect cell proliferation,disturb cell membrane integrity or cause apoptosis and necrosis of cells.Then,a two-component voltage sensor strategy based on fluorescence resonance energy transfer (FRET) between CdSe@ZnS-GSH QDs and the dipicrylamine (DPA) is successfully developed to monitor the membrane potential by the fluorescence of CdSe@ZnS-GSH QDs.This study offers a facile strategy for labeling plasma membrane and monitoring the membrane potential of cells and will hold great potential in the research of signaling within intact neuronal circuits.展开更多
Atomically precise gold nanoclusters (Au NCs) are an emerging class of quantum-sized nanomaterials. The intrinsic discrete electronic energy levels endowed them with fascinating electronic and optical properties [1]. ...Atomically precise gold nanoclusters (Au NCs) are an emerging class of quantum-sized nanomaterials. The intrinsic discrete electronic energy levels endowed them with fascinating electronic and optical properties [1]. However,the prepared Au NCs are often protected with hydrophobic ligands and are not dispersible in aqueous solutions, which restricts their biological applications. To overcome this limitation, some phase transfer agents have been devoted to transferring hydrophobic Au NCs into the aqueous solution[2]. However, the resulting Au NCs are generally heterogeneous and aggregated, which makes their subsequent biofunctionalization difficult to control.展开更多
Due to the wide range of potential applications for next-generation multi-functional devices,the flexible selfpowered photodetector(PD)with polarity-switchable behavior is essential but very challenging to be realized...Due to the wide range of potential applications for next-generation multi-functional devices,the flexible selfpowered photodetector(PD)with polarity-switchable behavior is essential but very challenging to be realized.Herein,a wearable bidirectional self-powered PD based on detached(Al,Ga)N and(In,Ga)N nanowires has been proposed and demonstrated successfully.Arising from the photovoltage-competing dynamics across(Al,Ga)N and(In,Ga)N nanowire photoelectrodes,such PD can generate the positive(33.3 mA W−1)and negative(-0.019 mA W−1)photo-responsivity under ultraviolet(UV)and visible illumination,respectively,leading to the bidirectional photocurrent behavior.Thanks to the introduction of quasi solid-state hydrogel,the PD can work without the liquid-electrolyte,thus remarkably reducing the volume from about 482 cm3 to only 0.18 cm3.Furthermore,the use of hydrogel is found to enhance response speed in the UV range by reducing the response time for more than 95%,which is mainly attributed to the increased open circuit potential and reduced ion transport distance.As the GaN connecting segment is pretty thin,the piezoelectric charges generated by stress are proposed to have only a limited effect on the photocurrent density.Therefore,both the stable on-off switching characteristics and photocurrent densities can still be achieved after being bent 400 times.With an excellent flexibility,this work creates opportunities for technological applications of bidirectional photocurrent PDs in flexible optoelectronic devices,e.g.,wearable intelligent sensors.展开更多
CONSPECTUS:Diamond nanomaterials have attracted significant interest in recent years due to their unique physical and chemical properties.Their exceptional mechanical strength,chemical stability,biocompatibility,and h...CONSPECTUS:Diamond nanomaterials have attracted significant interest in recent years due to their unique physical and chemical properties.Their exceptional mechanical strength,chemical stability,biocompatibility,and high thermal conductivity make them ideal candidates for a wide range of biomedical applications.Various formats,including nanodiamonds,diamond nanofilms,and diamond nanoneedle arrays(DNNAs),have been fabricated and used,exhibiting remarkable stability and low cytotoxicity.In particular,high-aspect-ratio and highdensity DNNAs demonstrate promising potential for live cell manipulation and analysis because of their unique combination of mechanical robustness,chemical stability,and wellforged bio−nanointerfaces.On the other hand,the chemical stability of diamond material makes fabrication and functionalization challenging,which could be improved for their wider adoption.展开更多
Water pollution is a serious problem around the world. It causes the lack of clean drinking water and brings risks to human health.Membrane technology has become a competitive candidate to treat the contaminated waste...Water pollution is a serious problem around the world. It causes the lack of clean drinking water and brings risks to human health.Membrane technology has become a competitive candidate to treat the contaminated wastewater due to its high separation efficiency and low energy consumption. In this review, we introduce the recent development of several kinds of bioinspired separation membranes, involving the membrane design and applications. We emphasize the multi-phase liquid separation membranes inspired from nature with special wettability applied for oil/water separation, organic liquids mixture separation, and emulsion separation. After separating multi-phase liquids using these membranes, small molecule pollutants still exist in singlephase liquid. Therefore, we also expand the scope to small molecule-scale separation membranes, such as the nacre-like graphene oxide separation membrane and other nanofiltration membranes. Summary and outlook concerning the future development of separation membranes are also introduced briefly.展开更多
Real-time tracking drug release behavior is fundamentally important for avoiding adverse effects or unsuccessful treatment in personalizemedical treatment.However,the development of a non-invasive drug reporting platf...Real-time tracking drug release behavior is fundamentally important for avoiding adverse effects or unsuccessful treatment in personalizemedical treatment.However,the development of a non-invasive drug reporting platform still remains challenging.Herein the design of a novelsyn thetic magnetic resonance imaging(MRI)agent for drug release tracking(SMART)is reported,which integrates photothermal core andparamagnetic ion/drug loading shell with a thermal valve in a hybrid structure.Through near-infrared(NIR)-II photothermal effect originatingfrom inner Au-Cu9S5 nanohybrid core,burst release of drugs loaded in the mesoporous silica shell is achieved.The concomitant use of aphase change material not only prevents premature drug release,but also regulates heating effect,keeping local temperature below 45℃,enabling synergistic chemotherapy and mild hyperthermia in vitro and in vivo.Furthermore,the drug release from SMART facilitates protonaccessibility to the paramagnetic ions anchored inside mesopores channels,enhancing Iongitudinal T1 relaxation rate and displaying positivesignal correlation to the amount of released drug,thus allowing norvinvasive real-time monitoring of drug release event.The current studyhighlights the potential of designed MRI nanophores such as SMART for real-time and in-situ monitoring of drug delivery for precisionthera nostic applications.展开更多
膜分离技术在有机分子污水的处理中具有广泛应用,其面临的难题之一是制备具有超亲水性质的纳孔膜以抵御有机污染物的污染,实现高效、稳定的分子分离.在本文中,我们通过全膜交联的方法在保持水凝胶纳孔膜超亲水性质的前提下大幅提升了其...膜分离技术在有机分子污水的处理中具有广泛应用,其面临的难题之一是制备具有超亲水性质的纳孔膜以抵御有机污染物的污染,实现高效、稳定的分子分离.在本文中,我们通过全膜交联的方法在保持水凝胶纳孔膜超亲水性质的前提下大幅提升了其机械强度,制备了高强度超亲水纳孔膜.该膜实现了有机分子的高效分离,水通量达到220 L m^(-2)h^(-1)bar^(-1),考马斯亮蓝(CBB)截留率达到~99.9%,分子/盐(CBB/Na_(2)SO_(4))选择性达到747.在以腐殖酸为污染物的分子分离中,该膜表现出优异的抗污染性能,通量恢复率接近100%.该膜还具有出色的抗压性能,在高达8 bar的跨膜压力下保持了稳定的分子分离性能.本工作为制备超亲水纳孔膜实现有机分子废水的高效分离提供了一种新的思路.展开更多
A simple and straightforward strategy for magnetic resonance imaging （MRI） of stem cell transplants in terms of their viability, migration and homing, and differentiation has been pursued over the years. Herein we c...A simple and straightforward strategy for magnetic resonance imaging （MRI） of stem cell transplants in terms of their viability, migration and homing, and differentiation has been pursued over the years. Herein we couple Gd-DOTA with triphenylphosphonium （TPP） to yield small molecule （Gd-DOTA）i-TPP （i = 1,4） agents and show that labeling cells with （Gd-DOTA）i-TPP via electro- poration （EP） results in two distinct cellular distributions of （Gd-DOTA）i-TPP： freely and evenly distributed in the cytosol or cell-assembled nanoclusters in the cytoplasm. The two distinct cellular distributions contribute in different ways to MRI signals in vitro and in vivo. Importantly, we present a detailed interpretation of MRI results based on the signal intensity equation and cellular longitudinal （T1-） and transverse （Ta-） relaxation rates of water protons. We demonstrate that cell-assembled （Gd-DOTA）i-TPP nanoclusters not only promote its intracellular retention time but also induce significant MRI signal reduction, which act as an excellent T2 contrast agent and allows for unambiguous reporting of in vivo viability and migration of cell transplants under T2-weighted MRI over a long period. Notably,（Gd-DOTA）i-TPP agents released as a result of exocytosis or cell death induce signal enhancement in the surrounding tissue such that the labeled cells can be unambiguously discriminated from its host tissue. The labeling and imaging strategy provides abundant information on the in vivo fates of stem cell transplants. The strategy features a single contrast, single imaging mode with dual signal output.展开更多
Almost all physiological processes of animals are controlled by the brain,including language,cognitive,memory,learning,emotion and so forth.Minor brain dysfunction usually leads to brain diseases and disorders.Therefo...Almost all physiological processes of animals are controlled by the brain,including language,cognitive,memory,learning,emotion and so forth.Minor brain dysfunction usually leads to brain diseases and disorders.Therefore,it'is greatly meaningful and urgent for scientists to have a better understanding of brain structure and function.Optical approaches can provide powerful tools for imaging and modulating physiological processes of the brain.In particular,optical approaches in the near-infrared(NIR)window(700-1700 nm)exhibit excellent prosperities of deep tissue penetration and low tissue scattering and absorption compared with those of visible windows(400-700 nm),which provides a promising approach for scientists to develop desired methods of neuroimaging and neuromodulation in deep brain tissues.In this review,variable types of NIR light approaches for imaging and modulating neural ions,membrane potential,neurotransmitters,and other critical molecules for brain functions and diseases are summarized.In particular,the latest breakthrough research of brain imaging and brain regulation in the NIR-II window(1000-1700 nm)are highlighted.Finally,we conclude the challenges and prospects of NIR light-based neuroimaging and neuromodulation for both basic brain research and further clinical translation.展开更多
基金the Natural Science Foundation of China(21673281,31870982)a National Key R&D Program from MOST of China(2017YFA0104301).
文摘Human mesenchymal stem cells(hMSCs)were labeled with Dotarem or(Gd-DOTA)2-EM7(EM7Gd2)via electroporation(EP).Cellular transmission electron microscopy(TEM)reveals free distribution of Gd agents and formation of EM7Gd2 clusters in the cytosol.Cellular magnetic resonance imaging(MRI)reveals that the free Gd agents induce MRI signal enhancement effect due to its fast exocytosis and subsequent interaction with intercellular water molecules.The EM7Gd2 clusters exhibits a longer intracellular retention time and induce a persistent MRI signal reduction effect.The cellular MRI results are interpreted by taking into account both T1 and T2 relaxation rates and their correlation with cellular binding structures of Dotarem and EM7Gd2.
基金supported by the National Natural Science Foundation of China(Nos.32071392,21775160 and 31900999)the Natural Science Foundation of Jiangsu Province(No.BE2020766)the Science Foundation of Jiangxi Province(No.20192ACB21033)。
文摘Aflatoxin B1(AFB1)is one of the most common mycotoxins that threatens human health.As singlestranded oligonucleotides with high affinity and specificity,aptamers have incomparable effect on the targeted detection of AFB1.Herein,after 11 rounds of selection and analysis using a modified affinity chromatography-based SELEX strategy,the truncated 37 nt aptamer AF11-2 was successfully obtained.The aptamer shows good detection performance for AFB1,and can sensitively detect AFB1 in the range of 100-1000 nmol/L,with a detection limit of 42 nmol/L.In the detection of pretreated edible peanut oil samples,AF11-2 aptamer also showed a high recovery rate and good stability for AFB1,and achieved satisfactory results.In addition,AF11-2 aptamer can significantly enhance the fluorescence ability of AFB1,which is not available in traditional Afla17-2-3 aptamer.After molecular docking analysis,it was found that AF11-2 and Afla17-2-3 had different nucleotide binding sites for AFB1.Afla17-2-3 binds to the carbonyl O of AFB1,while AF11-2 binds to the pyrrolic O of AFB1,which may be the main reason that AF11-2 can enhance the fluorescence of AFB1.
基金This work was supported by the National Natural Science Foundation of China(No.2210011636)the Natural Science Foundation of Jiangsu Province(No.BK20210311)+3 种基金China Postdoctoral Science Foundation(Nos.2021M691561 and 2021T140319)Jiangsu Planned Projects for Postdoctoral Research Funds(No.2021K547C)CAS Key Laboratory of Nano-Bio Interface(No.21NBI02)the Fundamental Research Funds for the Central Universities(No.NS2021037).
文摘Electrochemical CO_(2)reduction(ECR)is one of the most effective methods to obtain carbonaceous chemicals and reduce greenhouse gases passingly under the ambient condition.However,efficient electrocatalysts featured with high selectivity and stability are still lacking.A novel molecule-mediated Ag electrocatalyst with capped thiols is rationally designed for high-performance ECR.The thiol-capped and carbon-supported Ag nanostructures(Ag-TC)are formed by in situ electrochemical reduction from three-dimentional(3D)Ag-thiol metal-organic compound with cysteine as the anchor agent and carbon source.Ag-TC exhibits high selectivity and stability for CO_(2)conversion to CO(86.7%),which is more catalytically active than that of common Ag nanoparticles.The function of thiols for ECR is proved by replacing cysteine with alanine without thiol group.Meanwhile,alternatively replacing and removing the surface molecules on the Ag foil further demonstrate the effct of thiols.This work enlightens the promise of in situ construction method for molecule capped metal electrocatalyst towards selective and stable ECR.
基金the National Natural Science Foundation of China(No.2210011636)the Natural Science Foundation of Jiangsu Province(No.BK20210311)+3 种基金China Postdoctoral Science Foundation(Nos.2021M691561 and 2021T140319)Jiangsu Planned Projects for Postdoctoral Research Funds(No.2021K547C)CAS Key Laboratory of Nano-Bio Interface(No.21NBI02)the Fundamental Research Funds for the Central Universities(No.NS2021037).
文摘Erratum to Nano Research 2022,15(4):3283−3289 https://doi.org/10.1007/s12274-021-3978-7 One funding number in the Acknowledgements section was unfortunately mistakenly used.This error did not affect any of the conclusions from the published paper.
基金supported by the National Natural Science Foundation of China(Grant No.32171367)Natural Science Foundation of Jiangsu Province(Grant No.BK20230236)+1 种基金Science and Technology Project of Suzhou(Grant No.SS202135)CAS-VPST Silk Road Science Fund 2021(Grant No.121E32KYSB20200021).
文摘Mesenchymal stem cells(MSCs)have emerged as promising candidates for idiopathic pulmonary fibrosis(IPF)therapy.Increasing the MSC survival rate and deepening the understanding of the behavior of transplanted MSCs are of great significance for improving the efficacy of MSC-based IPF treatment.Therefore,dual-functional Au-based nanoparticles(Au@PEG@PEI@TAT NPs,AuPPT)were fabricated by sequential modification of cationic polymer polyetherimide(PEI),polyethylene glycol(PEG),and transactivator of transcription(TAT)penetration peptide on AuNPs,to co-deliver retinoic acid(RA)and microRNA(miRNA)for simultaneously enhancing MSC survive and real-time imaging tracking of MSCs during IPF treatment.AuPPT NPs,with good drug loading and cellular uptake abilities,could efficiently deliver miRNA and RA to protect MSCs from reactive oxygen species and reduce their expression of apoptosis executive protein Caspase 3,thus prolonging the survival time of MSC after transplantation.In themeantime,the intracellular accumulation of AuPPT NPs enhanced the computed tomography imaging contrast of transplantedMSCs,allowing them to be visually tracked in vivo.This study establishes an Au-based dual-functional platform for drug delivery and cell imaging tracking,which provides a new strategy for MSC-related IPF therapy.
基金supported by the National Key Research and Development Program of China(grant nos.2016YFA0101503 and 2017YFA0205503)the National Natural Science Foundation of China(grant nos.21934007,21778070,and 21671198)+2 种基金Chinese Academy of Sciences(grant nos.XDB32030200,121E32KYSB20180021,and ZDBS-LY-SLH021)the Natural Science Foundation of Jiangsu Province(grant no.BK20170066)Youth Innovation Promotion Association of Chinese Academy of Sciences。
文摘Acute myeloid leukemia(AML)remains a significant concern in modern medicine.Early diagnosis is the key to improving the therapeutic effects of AML.In the present work,a cascade-targeted and activatable NIR-Ⅱ nanoprobe(Ald&A1094@Ag_(2)S)was developed for early detection of AML in an orthotopic model.Upon intravenous injection,Ald&A1094@Ag_(2)S effectively accumulated in bone tissue due to its high affinity for alendronate(Ald)to the bone.Thereafter,the AML microenvironment allowed for the membrane-penetrating peptide TAT(cell‐penetrating peptide(CGRRRQRRKKRG))to be exposed via pH-sensitive hydrazone bond-mediated detaching of bone-targeted ligands,resulting in efficient internalization of nanoprobes in HL60 cells.Endogenous peroxynitrite(ONOO–)in HL60 cells further activated NIR-Ⅱ fluorescence of Ag_(2)S QDs via A1094 oxidation,thereby inhibiting fluorescence resonance energy transfer(FRET).Such a unique cascade-targeted and activatable strategy enables the nanoprobes to only light up the AML lesion region in the bone marrow with negligible background effects,which holds great potential for clinical applications in the future.
基金supported by Beijing Natural Science Foundation(No.JQ21003)the National Key Basic R&D Research Program of Ministry of Science and Technology of the People’s Republic of China(No.2021YFA1200303)the National Natural Science Foundation of China(Nos.22302046,22172041,52173159,92256304,and 22271308).
文摘The second near-infrared(NIR-II,1000-1700 nm)circularly polarized light holds significant untapped potential in areas such as optical anti-counterfeiting and information encryption due to its deeply covert nature.However,the typically low luminescence dissymmetry factor(glum)of circularly polarized luminescence(CPL)materials,particularly in NIR CPL materials,limits their practical application.Addressing this challenge,it is crucial to develop NIR CPL materials with enhanced glum.In this study,we present a series of chiral photonic micro-particles(CPMPs)with tunable chiral photonic bandgaps in the NIR-II range,capable of modulating NIR-II luminescent quantum dots to produce NIR CPL.These CPMPs not only impart chirality to the quantum dots,but also act as carriers,minimizing luminescence quenching from external environments.The tunable chiral photonic bandgap in CPMPs enables the generation of NIR CPL with a high|glum|value of up to 0.81,facilitating the advanced application in covert optical anti-counterfeiting.This work offers a straightforward and viable strategy for the development of NIR CPL materials,broadening their use in invisible information encryption and optical anti-counterfeiting technologies.
基金the National Key Research and Development Program of China(No.2021YFF0701804)the financial support from the National Natural Science Foundation of China(Nos.21934007,22001262,22177128,and 22271308)+1 种基金the Science and Technology Project of Suzhou(No.SZS201904)the Natural Science Foundation of Jiangsu Province(Nos.BK20222016,BK20200254,and BK20221262).
文摘Cation exchange(CE)has been emerged as a promising post-synthesis strategy of colloidal nanocrystals.However,it is unclear how the cation precursor affects the CE process and the final colloidal nanocrystals.Herein,we utilized two Zn-B Lewis acidbase adduct complexes(B=oleylamine(OAM)and methanol(MeOH))as Zn precursors for CE with Ag_(2)S quantum dots(QDs).Our study revealed that the steric hindrance and complexing capabilities of Zn precursor significantly affect the CE kinetics.As a result,the Zn-doped Ag_(2)S(Zn:Ag_(2)S)and Ag_(2)S@ZnS core–shell QDs were successfully obtained with enormous enhancement of their photoluminescence(PL)intensities.Theoretical simulation showed that the Zn-OAM with higher desolvation energy and spatial hindrance tended to form doped Zn:Ag_(2)S QDs due to the inefficient cation exchange.Whereas the Zn-MeOH with lower exchange barrier promoted the conversion of Ag-S to Zn-S,thus forming Ag_(2)S@ZnS core–shell QDs.We anticipate that this finding will enrich the regulatory approaches of post-synthesis of colloidal nanocrystals with desirable properties.
基金supported by the National Natural Science Foundation of China(Nos.21977112,and 21934007)the Natural Science Foundation of Jiangsu Province(No.BK20190227)+1 种基金the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDB36000000)the Science and Technology Project of Suzhou(No.SZS201904).
文摘Significant chiroptical responses could be generated by chiral coupling of achiral plasmonic nanoparticles,or originated from intrinsically chiral plasmonic nanoparticles.Here we create dimeric plasmonic metamolecules possessing both chiral coupling between nanoparticles and intrinsic chiroptical responses derived from nanoparticles themselves.These plasmonic metamolecules are prepared by assembling helical plasmonic nanorods(HPNRs)with intrinsic chirality in chiral manners on DNA origami template.Two HPNRs with the same or opposite chirality,or one HPNR and one achiral gold nanorod,are coupled chirally into dimeric metamolecules with intriguing plasmonic circular dichroism(PCD).We found that both of the intrinsic chirality of constituent HPNRs and the chiral coupling contribute to the overall PCD while their weights are different in different metamolecules and vary in different wavelength range for a certain metamolecule.Comparing to conventional chiral plasmonic metamolecules from achiral nanoparticles,or discrete chiral nanoparticles,these metamolecules bring more dimensions for tailoring chiroptical responses and make it more flexible to design plasmonic nanodevices with custom PCD.
基金the National Natural Science Foundation of China(Nos.21934007 and 22001262)the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDB36000000)+1 种基金the Natural Science Foundation of Jiangsu Province(No.BK20200254)China Postdoctoral Science Foundation(No.2019M661966).
文摘Surface ligands of colloidal quantum dots(QDs)have a profound influence on their surface states,which has been verified in the studies of the effect of ligand head groups on the photoluminescence(PL)properties of QDs.However,the investigation of the ligand chain length is limited.Here,we systematically explored the effect of chain length on the Ag_(2)Se QDs by selecting three ligands,1-octanethiol(OTT),1-dodecanethiol(DDT),and 1-hexadecanethiol(HDT),with diverse chain lengths.We found that the PL intensity of Ag_(2)Se QDs increased with the decrease of the ligand chain length due to the enhanced passivation of surface defects emerging from the robust QD-ligand interface binding affinity and the weaker hydrophobic chain–chain interaction.Subsequently,AgAuSe QDs terminated with OTT were obtained by alloying parent OTT-Ag_(2)Se QDs with Au precursor with a record absolute PL quantum yield(PLQY)of 87.2%at 970 nm,facilitating ultrasensitive in vivo angiography imaging in a nude mouse model.We expect that our finding of the important role of the ligand chain length on the optical properties of QDs will be suggestive to the design and synthesis of high-quality QDs,and also look forward to the clinical applications of the ultra-bright AgAuSe QDs.
基金the Strategic Priority Research Program (No.XDB32030200)Youth Innovation Promotion Association Program from Chinese Academy of Sciences+2 种基金the National Key Research and Development Program (Nos.2016YFA0101503 and 2017YFA0205503)the National Natural Science Foundation of China (Nos.21778070,2167119& 21425103,and 21501192)the National Natural Science Foundation of Jiangsu Province (Nos.BK20170066 and BE2016682).
文摘The plasma membrane of cells is a crucial biological membrane that involved in a variety of cellular processes including cell signaling transduction through membrane electrical activity.Recently,monitoring membrane electrical activity using fluorescence imaging has attracted numerous attentions for its potential applications in evaluating how the nervous system works.However,the development of ideal fluorescent voltage-sensitive probes with both high membrane labeling efficiency and voltage sensitivity is still retain a big challenge.Herein,glutathionecapped CdSe@ZnS quantum dots (CdSe@ZnS-GSH QDs) with a size of 2.5 nm and an emission peak at 520 nm are synthesized using a facile ligand exchange method for plasma membrane labeling and membrane potential imaging.The as-synthesized CdSe@ZnS-GSH QDs can effectively label cell membrane at neutral pH within 30 min and exhibit excellent optical stability in continuous imaging for up to 60 min.With the test concentration up to 200 nM,CdSe@ZnS-GSH QDs show high biocompatibility to cells and do not affect cell proliferation,disturb cell membrane integrity or cause apoptosis and necrosis of cells.Then,a two-component voltage sensor strategy based on fluorescence resonance energy transfer (FRET) between CdSe@ZnS-GSH QDs and the dipicrylamine (DPA) is successfully developed to monitor the membrane potential by the fluorescence of CdSe@ZnS-GSH QDs.This study offers a facile strategy for labeling plasma membrane and monitoring the membrane potential of cells and will hold great potential in the research of signaling within intact neuronal circuits.
文摘Atomically precise gold nanoclusters (Au NCs) are an emerging class of quantum-sized nanomaterials. The intrinsic discrete electronic energy levels endowed them with fascinating electronic and optical properties [1]. However,the prepared Au NCs are often protected with hydrophobic ligands and are not dispersible in aqueous solutions, which restricts their biological applications. To overcome this limitation, some phase transfer agents have been devoted to transferring hydrophobic Au NCs into the aqueous solution[2]. However, the resulting Au NCs are generally heterogeneous and aggregated, which makes their subsequent biofunctionalization difficult to control.
基金National Natural Science Foundation of China(62174172,61875224 and 61827823)Key Research and Development Program of Jiangsu Province(BE2018005)+4 种基金Key Research Program of Frontier Sciences,CAS(ZDBS-LY-JSC034)support from Natural Science Foundation of Jiangxi Province(20192BBEL50033)Research Program of Scientific Instrument and Equipment of CAS(YJKYYQ20200073)SINANO(Y8AAQ21001)support from Vacuum Interconnected Nanotech Workstation(Nano-X,F2201),Platform for Characterization&Test of SINANO,CAS.
文摘Due to the wide range of potential applications for next-generation multi-functional devices,the flexible selfpowered photodetector(PD)with polarity-switchable behavior is essential but very challenging to be realized.Herein,a wearable bidirectional self-powered PD based on detached(Al,Ga)N and(In,Ga)N nanowires has been proposed and demonstrated successfully.Arising from the photovoltage-competing dynamics across(Al,Ga)N and(In,Ga)N nanowire photoelectrodes,such PD can generate the positive(33.3 mA W−1)and negative(-0.019 mA W−1)photo-responsivity under ultraviolet(UV)and visible illumination,respectively,leading to the bidirectional photocurrent behavior.Thanks to the introduction of quasi solid-state hydrogel,the PD can work without the liquid-electrolyte,thus remarkably reducing the volume from about 482 cm3 to only 0.18 cm3.Furthermore,the use of hydrogel is found to enhance response speed in the UV range by reducing the response time for more than 95%,which is mainly attributed to the increased open circuit potential and reduced ion transport distance.As the GaN connecting segment is pretty thin,the piezoelectric charges generated by stress are proposed to have only a limited effect on the photocurrent density.Therefore,both the stable on-off switching characteristics and photocurrent densities can still be achieved after being bent 400 times.With an excellent flexibility,this work creates opportunities for technological applications of bidirectional photocurrent PDs in flexible optoelectronic devices,e.g.,wearable intelligent sensors.
基金supported by the National Nature Science Foundation of China(52172241,52173242,32201176,U20A20194)Hong Kong Research Grants Council(11215920,11218522,11218523,11308321,11308120)+2 种基金Guangdong Basic and Applied Basic Research Foundation(2019B1515120005)Shenzhen Science and Technology Planning Project(JCYJ20200109115424940)Science and Technology Foundation of Suzhou(ZXL2023203).
文摘CONSPECTUS:Diamond nanomaterials have attracted significant interest in recent years due to their unique physical and chemical properties.Their exceptional mechanical strength,chemical stability,biocompatibility,and high thermal conductivity make them ideal candidates for a wide range of biomedical applications.Various formats,including nanodiamonds,diamond nanofilms,and diamond nanoneedle arrays(DNNAs),have been fabricated and used,exhibiting remarkable stability and low cytotoxicity.In particular,high-aspect-ratio and highdensity DNNAs demonstrate promising potential for live cell manipulation and analysis because of their unique combination of mechanical robustness,chemical stability,and wellforged bio−nanointerfaces.On the other hand,the chemical stability of diamond material makes fabrication and functionalization challenging,which could be improved for their wider adoption.
基金supported by the National Natural Science Foundation of China(21433012,21774005,21374001,21503005,51772010)the National Instrumentation Program(2013YQ120355)+3 种基金the Program for New Century Excellent Talents in University of Chinathe Fundamental Research Funds for the Central Universitiesthe National Program for Support of Top-notch Young Professionalsthe Program of Introducing Talents of Discipline to Universities of China(B14009)
文摘Water pollution is a serious problem around the world. It causes the lack of clean drinking water and brings risks to human health.Membrane technology has become a competitive candidate to treat the contaminated wastewater due to its high separation efficiency and low energy consumption. In this review, we introduce the recent development of several kinds of bioinspired separation membranes, involving the membrane design and applications. We emphasize the multi-phase liquid separation membranes inspired from nature with special wettability applied for oil/water separation, organic liquids mixture separation, and emulsion separation. After separating multi-phase liquids using these membranes, small molecule pollutants still exist in singlephase liquid. Therefore, we also expand the scope to small molecule-scale separation membranes, such as the nacre-like graphene oxide separation membrane and other nanofiltration membranes. Summary and outlook concerning the future development of separation membranes are also introduced briefly.
基金This work was funded by the National Natural Science Foundation of China(No.21473243)Six Talent Peaks Project in Jiangsu Province(No.SWYY-243).
文摘Real-time tracking drug release behavior is fundamentally important for avoiding adverse effects or unsuccessful treatment in personalizemedical treatment.However,the development of a non-invasive drug reporting platform still remains challenging.Herein the design of a novelsyn thetic magnetic resonance imaging(MRI)agent for drug release tracking(SMART)is reported,which integrates photothermal core andparamagnetic ion/drug loading shell with a thermal valve in a hybrid structure.Through near-infrared(NIR)-II photothermal effect originatingfrom inner Au-Cu9S5 nanohybrid core,burst release of drugs loaded in the mesoporous silica shell is achieved.The concomitant use of aphase change material not only prevents premature drug release,but also regulates heating effect,keeping local temperature below 45℃,enabling synergistic chemotherapy and mild hyperthermia in vitro and in vivo.Furthermore,the drug release from SMART facilitates protonaccessibility to the paramagnetic ions anchored inside mesopores channels,enhancing Iongitudinal T1 relaxation rate and displaying positivesignal correlation to the amount of released drug,thus allowing norvinvasive real-time monitoring of drug release event.The current studyhighlights the potential of designed MRI nanophores such as SMART for real-time and in-situ monitoring of drug delivery for precisionthera nostic applications.
基金financially supported by the National Key Research and Development Plan of China(2019YFA0705800)。
文摘膜分离技术在有机分子污水的处理中具有广泛应用,其面临的难题之一是制备具有超亲水性质的纳孔膜以抵御有机污染物的污染,实现高效、稳定的分子分离.在本文中,我们通过全膜交联的方法在保持水凝胶纳孔膜超亲水性质的前提下大幅提升了其机械强度,制备了高强度超亲水纳孔膜.该膜实现了有机分子的高效分离,水通量达到220 L m^(-2)h^(-1)bar^(-1),考马斯亮蓝(CBB)截留率达到~99.9%,分子/盐(CBB/Na_(2)SO_(4))选择性达到747.在以腐殖酸为污染物的分子分离中,该膜表现出优异的抗污染性能,通量恢复率接近100%.该膜还具有出色的抗压性能,在高达8 bar的跨膜压力下保持了稳定的分子分离性能.本工作为制备超亲水纳孔膜实现有机分子废水的高效分离提供了一种新的思路.
基金This work was funded by general projects from the National Natural Science Foundation of China （Nos. 21673281 and 31371010）, a Strategic Priority Research Program of the Chinese Academy of Sciences （No. XDA01030203）, and a Basic Research Project from the Ministry of Science and Technology of China （No. 2011CB965004）. The authors also acknowledge Karebay Biochem Inc. for assistance with synthesis of DOTArTPP.
文摘A simple and straightforward strategy for magnetic resonance imaging （MRI） of stem cell transplants in terms of their viability, migration and homing, and differentiation has been pursued over the years. Herein we couple Gd-DOTA with triphenylphosphonium （TPP） to yield small molecule （Gd-DOTA）i-TPP （i = 1,4） agents and show that labeling cells with （Gd-DOTA）i-TPP via electro- poration （EP） results in two distinct cellular distributions of （Gd-DOTA）i-TPP： freely and evenly distributed in the cytosol or cell-assembled nanoclusters in the cytoplasm. The two distinct cellular distributions contribute in different ways to MRI signals in vitro and in vivo. Importantly, we present a detailed interpretation of MRI results based on the signal intensity equation and cellular longitudinal （T1-） and transverse （Ta-） relaxation rates of water protons. We demonstrate that cell-assembled （Gd-DOTA）i-TPP nanoclusters not only promote its intracellular retention time but also induce significant MRI signal reduction, which act as an excellent T2 contrast agent and allows for unambiguous reporting of in vivo viability and migration of cell transplants under T2-weighted MRI over a long period. Notably,（Gd-DOTA）i-TPP agents released as a result of exocytosis or cell death induce signal enhancement in the surrounding tissue such that the labeled cells can be unambiguously discriminated from its host tissue. The labeling and imaging strategy provides abundant information on the in vivo fates of stem cell transplants. The strategy features a single contrast, single imaging mode with dual signal output.
基金National Natural Science Foundation of China,Grant/Award Numbers:22127808,22177128,22204172,21934007,22174158the National Key Research and Development Program,Grant/Award Number:2021YFF0701804+2 种基金the Science and Technology Project of Suzhou,Grant/Award Numbers:SZS201904,SJC2021001the Natural Science Foundation of Jiangsu Province,Grant/Award Numbers:BK20222016,BE2022753,BE2022745Chinese Academy of Sciences,Grant/Award Numbers:YJKYYQ20200036,121E32KYSB20180021,ZDBS-LY-SLH021。
文摘Almost all physiological processes of animals are controlled by the brain,including language,cognitive,memory,learning,emotion and so forth.Minor brain dysfunction usually leads to brain diseases and disorders.Therefore,it'is greatly meaningful and urgent for scientists to have a better understanding of brain structure and function.Optical approaches can provide powerful tools for imaging and modulating physiological processes of the brain.In particular,optical approaches in the near-infrared(NIR)window(700-1700 nm)exhibit excellent prosperities of deep tissue penetration and low tissue scattering and absorption compared with those of visible windows(400-700 nm),which provides a promising approach for scientists to develop desired methods of neuroimaging and neuromodulation in deep brain tissues.In this review,variable types of NIR light approaches for imaging and modulating neural ions,membrane potential,neurotransmitters,and other critical molecules for brain functions and diseases are summarized.In particular,the latest breakthrough research of brain imaging and brain regulation in the NIR-II window(1000-1700 nm)are highlighted.Finally,we conclude the challenges and prospects of NIR light-based neuroimaging and neuromodulation for both basic brain research and further clinical translation.
