Herein,cure characteristics,morphology,and mechanical properties of natural rubber filled with activated carbon-based materials were investigated.Carbon-based materials were prepared from bagasse,coffee grounds and pi...Herein,cure characteristics,morphology,and mechanical properties of natural rubber filled with activated carbon-based materials were investigated.Carbon-based materials were prepared from bagasse,coffee grounds and pineapple crowns by the pyrolysis method at temperatures in the range of 300℃.As-synthesized carbon materials were characterized by optical microscopy(OM),scanning electron microscopy(SEM),and Fourier-transform infrared spectroscopy(FTIR)to analyze size distribution,morphology,and functional groups,respectively.OM and SEM analysis revealed that particles,flakes,and a small quantity of fiber-like carbon were obtained using bagasse and pineapple crown as raw materials,while honeycomb-like carbon materials can be derived from coffee grounds.To investigate the mechanical properties,natural rubber was filled with carbon black and as-synthesized carbon materials by the internal mixing and compression molding process.Transmission electron microscopy(TEM)was utilized to characterize the dispersion of carbon materials in the rubber matrix.The results of tensile testing showed that the natural rubber mixed with as-synthesized carbon materials from pineapple crowns exhibited 54%and 74%improvement in the ultimate tensile strength and Young’s modulus,respectively,compared with natural rubber without filled carbon materials.The enhancement in mechanical properties by activated carbon materials derived from pineapple crowns can be attributed to the flake-and fiber-like structures and good dispersion of carbon materials in the rubber matrix.In addition,it is higher than that of rubber mixed with carbon black.The results demonstrated that as-synthesized carbon materials from pineapple crowns have the potential materials to substitute carbon black in the rubber compound industry.展开更多
Background:People working outdoors in the Map Ta Phut pollution control area of Thailand require comprehen-sive health monitoring.In the past,studies have been done on the health effects of pollutants.However,there ar...Background:People working outdoors in the Map Ta Phut pollution control area of Thailand require comprehen-sive health monitoring.In the past,studies have been done on the health effects of pollutants.However,there are few studies on musculoskeletal disorders(MSDs),and Thailand is struggling to meet the Sustainable Development Goals.Methods:This cross-sectional study examines access to health services and factors affecting MSDs among outdoor pollution workers(OPWs).The sample group includes OPWs,including local fisherman,street vendors,public car drivers,and traffic police.We studied 50 people from each of these groups,for a total of 200 people.Data were analyzed with inferential statistics using Chi-square test,McNemar test,and Univariate logistic regression.Results:The OPWs reported experiencing significantly more total MSDs pain than they did in the past(P<0.05).Factors affecting current MSDs pain,including occupation and working days per week,were significant(P<0.05).The street vendor group and public car driver group had(odds ratio[OR]=2.253,95%confidence interval[CI]:1.101 to 5.019)and(OR=2.681,95%CI:1.191 to 6.032)times higher risks of MSDs pain,respectively.OPWs who work>5 days per week had a(OR=1.464,95%CI:1.093 to 2.704)times higher risk of MSDs pain.52.7%of OPWs with MSDs,pain(n=110)had received an annual health check-up.In the past year,50.9%had minor illnesses and 21.8%had severe illnesses.OPWs receiving free treatment and visiting health service stations for no cost comprised 77.3%and 51.8%,respectively.60.9%used their right to receive treatment with universal health insurance cards.Conclusions:The study indicates that occupational groups with MSDs pain problems should exercise this right,according to the worker protection law.Local health agencies should organize activities or create accessible media to promote preventive medicine services,as many OPWs believe that health services can only be accessed when illness occurs.展开更多
This study examines how demographic factors shape engagement with virtual cultural communities and awareness of emerging economic models.Using survey data from 202 participants,descriptive statistics,independent sampl...This study examines how demographic factors shape engagement with virtual cultural communities and awareness of emerging economic models.Using survey data from 202 participants,descriptive statistics,independent samples t-tests,and One-way Analysis of Variance(ANOVA)with Least Significant Difference(LSD)post-hoc analyses revealed significant influences of age,education,and occupation.No significant gender differences were found.Individuals aged 26-45 demonstrated higher virtual community participation(F=3.439,p=0.01)and emerging model awareness(F=2.834,p=0.026)than younger cohorts(18-25 years and below).Respondents with postgraduate education exhibited superior understanding of emerging economic models(F=3.296,p=0.022)and their nexus with cultural values(F=6.196,p<0.001)compared to those with lower educational attainment.Significant occupational variations existed in virtual participation(F=4.001,p=0.008)and economic model awareness(F=5.611,p=0.001),with enterprise employees and civil servants scoring higher than students and freelancers.These findings underscore the critical roles of life stage,educational investment,and professional context in shaping digital cultural behaviors and economic cognition,offering valuable insights for platform design,educational strategies,and policy development in the digital cultural economy.展开更多
Globalization triggers interactive effects between educational inequality and economic development differences,which are influenced by complex dynamics and feedback mechanisms.This paper uses a comprehensive literatur...Globalization triggers interactive effects between educational inequality and economic development differences,which are influenced by complex dynamics and feedback mechanisms.This paper uses a comprehensive literature review and qualitative case analysis method to deeply explore the status,impact and joint feedback cycles of this phenomenon,focusing on California and Bangalore.The results reveal its interactive driving forces and provide important perspectives for understanding this issue.At the same time,the study proposes forward-looking policy recommendations and emphasizes the need for further exploration,especially around the role of cultural factors in curbing global inequality.展开更多
ESG(Environment,Society,Governance)management is being applied in the world’s industries,including ports,to enhance sustainable development.For the port sector,the world’s ports have increasingly adopted policies to...ESG(Environment,Society,Governance)management is being applied in the world’s industries,including ports,to enhance sustainable development.For the port sector,the world’s ports have increasingly adopted policies to drive ESG projects in alignment with sustainable port development goals.This research aims to identify port sustainability indicators and analyze the interrelationship among ESG dimensions related to the port environment,port society,and port governance.Qualitative and quantitative methods are employed to identify port management indicators that affect the operation of cargo ports,utilizing a systematic document analysis tool.A wide range of academic and institutional sources was reviewed to ensure the comprehensive identification of relevant indicators.The finding indicates that twenty-nine port sustainability indicators affect port sustainability management,including the nine indicators of environmental port,the nine indicators of social port,and the eleven indicators of governance port.These critical indicators reflect both global best practices and context-specific needs,and they play a central role in managing the port’s sustainability and guiding control strategies for improving competitiveness and compliance with international standards.Based on these findings,a port sustainability framework was developed as a practical operational tool to evaluate port performance,guide decision-making,and align port operations with Thailand’s national policies and global sustainability standards.This framework also provides a foundation for future empirical validation and offers practical implications for policymakers and port stakeholders.展开更多
Nitrogen-doped activated carbon(N-AC)was successfully prepared by KOH-activation and nitrogen doping using ammonia(NH3)heat treatment.Coconut shell-derived activated carbon(AC)was heat-treated under NH3 gas in the tem...Nitrogen-doped activated carbon(N-AC)was successfully prepared by KOH-activation and nitrogen doping using ammonia(NH3)heat treatment.Coconut shell-derived activated carbon(AC)was heat-treated under NH3 gas in the temperature range of 700℃-900℃.Likewise,the mixture of potassium hydroxide(KOH)and AC was heated at 800℃,followed by heat treatment underNH3 gas at 800℃(hereafter referred to asKOH-N-AC800).Scanning electron microscopy(SEM),Raman spectroscopy,X-ray photoelectron spectroscopy(XPS)and Brunauer-Emmett-Teller(BET)method were utilized to analyze morphology,crystallinity,chemical bonding,chemical composition and surface area.The surface area and porosity of N-AC increased with increasing NH3 heat treatment.Similarly,the nitrogen content in the N-AC increased from 3.23%to 4.84 at%when the NH3 heat treatment was raised from 700℃ to 800℃.However,the nitrogen content of N-AC decreased to 3.40 at% after using NH3 heat treatment at 900℃.The nitrogen content of KOH-N-AC800 is 5.43 at%.KOH-N-AC800 and N-AC800 exhibited improvements of 33.66% and 26.24%,respectively,in CO_(2) adsorption compared with AC.The enhancement of CO_(2) adsorption of KOH-N-AC800 is attributed to the synergic effect of the nitrogen doping,high surface area,and porosity.The results exhibited that nitrogen sites on the surface play a more significant role in CO_(2) adsorption than surface area and porosity.This work proposes the potential synergistic effect of KOH-activation and nitrogen doping for enhancing the CO_(2) adsorption capacity of activated carbon.展开更多
Objective:The present study investigated the cytoprotective effects of a Pogonatherum paniceum extract prepared with 80%ethanol(PPE)using synchrotron radiation-based Fourier transform infrared(SR-FTIR)microspectroscop...Objective:The present study investigated the cytoprotective effects of a Pogonatherum paniceum extract prepared with 80%ethanol(PPE)using synchrotron radiation-based Fourier transform infrared(SR-FTIR)microspectroscopy and determined its phytochemical profile.Methods:The volatile and polyphenolic compounds in PPE were characterized using gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry,respectively.The antioxidant capacity of PPE was evaluated using chemical and cell-based assays.The SR-FTIR microspectroscopy was performed to evaluate the cytoprotective effect of PPE by identifying changes in macromolecule composition in tert-butyl hydroperoxide(t BuOOH)-induced oxidative damage in RAW264.7 cells.Results:A total of 48 volatile compounds and 28 polyphenol components were found in PPE.PPE exhibited a high potential for antioxidant activity by scavenging the intracellular reactive oxygen species in t Bu OOH-induced oxidative damage in RAW264.7 cells.PPE treatment also significantly protected RAW264.7 cells against t BuOOH-induced toxicity and restored cell viability.The SR-FTIR analysis revealed that t BuOOH increased the lipid and ester lipid content in RAW264.7 cells.The PPE exerted a cytoprotective effect by decreasing the levels of lipid and ester lipid compounds that had been elevated by t BuOOH in RAW264.7 cells.These findings indicate that PPE has cytoprotective potential due to its ability to inhibit endogenous reactive oxygen species.Conclusion:This study extends the current knowledge on the phytochemistry of PPE and its antioxidant and cytoprotective effects.These findings support the use of SR-FTIR microspectroscopy to determine the cytoprotective effects of natural products.PPE extract may be a candidate compound for new therapeutics and nutraceuticals that target the prevention of oxidative stress-associated diseases.展开更多
BACKGROUND Magnesium(Mg^(2+))plays a fundamental role in numerous cellular processes,including enzymatic reactions,DNA replication,oxidative stress response,and cytoskeletal dynamics.In fact,dysregulation of Mg^(2+)ho...BACKGROUND Magnesium(Mg^(2+))plays a fundamental role in numerous cellular processes,including enzymatic reactions,DNA replication,oxidative stress response,and cytoskeletal dynamics.In fact,dysregulation of Mg^(2+)homeostasis has been increasingly associated with the development and progression of cancer,particularly colorectal cancer(CRC).Transient receptor potential melastatin(TRPM)channels,especially TRPM6 and TRPM7,are essential regulators of epithelial Mg^(2+)influx.While TRPM7 promotes CRC progression,the role of TRPM6 and TRPM6/7 channels remains unclear.AIM To investigate the role of membrane-localized TRPM6 and TRPM6/7 channels in Mg^(2+)influx,spheroid(SP)formation,stemness,and migration.METHODS We used parental and SP-derived HT-29 cells at comparable passages as in vitro models.Mass spectrometry confirmed full-length sequences,phosphorylation,and methionine oxidation of TRPM6 and TRPM7.Mg^(2+)influx,total and free Mg^(2+)levels were measured by fluorescence imaging and biochemical assays.TRPM6/TRPM7 expression and markers were analyzed by western blot.Func-tional assays,including secondary SP formation and wound healing,assessed stemness and migration.Cells were treated with Mg^(2+)transport inhibitors:Co(III)hexamine,2-aminoethyl diphenylborinate(TRPM6/7 blocker),and Mesendogen(TRPM6 inhibitor).RESULTS The expression of membrane-bound TRPM6,TRPM7,and TRPM6/7 was significantly higher in SP cells than in parental cells.Mass spectrometric analysis confirmed the presence of full-length TRPM6 and TRPM7 with increased phosphorylation and oxidation in SP cells.Enhanced Mg^(2+)influx and total intracellular Mg^(2+)levels were observed in SP cells.Free ionized intracellular Mg^(2+)levels remained comparable across all experimental groups.Pharmacological inhibition of TRPM6 and TRPM6/7 significantly reduced Mg^(2+)influx,decreased total Mg^(2+)content,compromised CRC SP stability,abolished cancer stem-like properties,impaired cell migration,and downregulated pro-tumorigenic markers,including Nanog,cyclooxygenase-2,and matrix metalloproteinase-9.CONCLUSION Membrane-localized TRPM6 and TRPM6/7 channels regulate Mg^(2+)influx and promote CRC stemness,SP stability,and migration,highlighting their potential as therapeutic targets to inhibit CRC progression and metastasis.展开更多
Objectives: To determine the level of adversity quotient and its associations with rumination, optimism, and authoritative parenting style in junior high school students. Adversity quotient is the adaptation ability t...Objectives: To determine the level of adversity quotient and its associations with rumination, optimism, and authoritative parenting style in junior high school students. Adversity quotient is the adaptation ability to overcome problems and obstacles with a positive attitude. Early adolescence, as a critical stage of life, needs to cultivate adversity quotient. However, adversity quotient and its influencing factors in adolescents have not been fully understood. Methods: A total of 129 students from 3 junior high schools (grade 7-9) in Chon Buri province, Thailand, were randomly recruited to participate in this correlational research study. They completed 5 questionnaires including the demographic form, adversity quotient assessment, authoritative parenting style, optimism, and rumination. Descriptive statistics and Pearson’s correlation coefficient were used for data analysis. Results: Adversity quotient was at a moderate level with a mean of 138.78 (standard deviation [SD] = 21.25). Adversity quotient was significantly, positively correlated with authoritative parenting at the highest level (r = 0.60), followed by optimism at a moderate level (r = 0.38), and rumination at a low level (r = 0.25, P < 0.01). Conclusions: All parties responsible for early adolescents’ development should enhance the adversity quotient to a higher level through campaigns promoting authoritative parenting and optimism. An unexpected positive correlation between adversity quotient and rumination warrants cautious interpretation and more studies.展开更多
Osteoporosis,a global age-related health problem in both male and female elderly,insidiously deteriorates the microstructure of bone,particularly at trabecular sites,such as vertebrae,ribs and hips,culminating in frag...Osteoporosis,a global age-related health problem in both male and female elderly,insidiously deteriorates the microstructure of bone,particularly at trabecular sites,such as vertebrae,ribs and hips,culminating in fragility fractures,pain and disability.Although osteoporosis is normally associated with senescence and estrogen deficiency,diabetes mellitus(DM),especially type 1 DM,also contributes to and/or aggravates bone loss in osteoporotic patients.This topic highlight article focuses on DM-induced osteoporosis and DM/ osteoporosis comorbidity,covering alterations in bone metabolism as well as factors regulating bone growth under diabetic conditions including,insulin,insulin-like growth factor-1 and angiogenesis.Cellular and molecular mechanisms of DM-related bone loss are also discussed.This information provides a foundation for the better understanding of diabetic complications and for development of early screening and prevention of osteoporosis in diabetic patients.展开更多
It was previously understood that body weight gain and obesity observed in type 2 diabetes mellitus(T2DM) could be beneficial since body weight increase elevated bone mineral density and thus helped maintain the skele...It was previously understood that body weight gain and obesity observed in type 2 diabetes mellitus(T2DM) could be beneficial since body weight increase elevated bone mineral density and thus helped maintain the skeletal framework.However,a number of recent findings in humans and rodents have revealed that T2 DM is not only associated with trabecular defects but also increases cortical porosity,and compromised bone cell function and bone mechanical properties.Hyperglycemia and insulin resistance in T2 DM may further induce osteoblast apoptosis and uncoupling bone turnover.Prolonged accumulation of advanced glycation end products and diminished activity of lysyl oxidase,an essential enzyme for collagen cross-link,can lead to structural abnormalities of bone collagen fibrils,brittle matrix,and fragility fractures.Our studies in T2 DM rats showed that dyslipidemia,which often occurs in T2 DM,could obscure the T2DM-associated changes in bone microstructure and osteopenia.Longitudinal bone growth regulated by the growth plate chondrocytes is also impaired by T2 DM since differentiation of growth plate chondrocytes is arrested and retained in the resting state while only a small number of cells undergo hypertrophic differentiation.Such a delayed chondrocyte differentiation may have also resulted from premature apoptosis of the growth plate chondrocytes.Nevertheless,the underlying cellular and molecular mechanisms of insulin resistance in osteoblasts,osteoclasts,osteocytes,and growth plate chondrocytes remain to be investigated.展开更多
A coagulation-flocculation process is typically employed to treat the industrial wastewater generated by the consumer products industry manufacturing detergents, soaps, and others. The expenditure of chemicals includi...A coagulation-flocculation process is typically employed to treat the industrial wastewater generated by the consumer products industry manufacturing detergents, soaps, and others. The expenditure of chemicals including coagulants and chemicals for pH adjustment is costly for treating this wastewater. The objective of this study was to evaluate the feasibility of reusing the aluminum sulfate （alum） sludge as a coagulant or as a coagulation aid so that the fresh alum dosage can be minimized or the removal efficiency can be enhanced. The experiments were conducted in a jar-test apparatus simulating the coagulation-flocculation process for simultaneous removals of organic matters, anionic surfactants, suspended solids, and turbidity. At the optimum initial pH value of 10 and the fresh alum concentration of 400 mg/L, the total suspended solids （TSS）, total chemical oxygen demand （TCOD）, total anionic surfactants, and turbidity removal efficiencies were 71.5%, 76.4%, 95.4%, and 98.2%, respectively. The addition of alum sludge as a coagulant alone without any fresh alum addition could significantly remove the turbidity, TCOD, and anionic surfactants. The TSS was left in the supernatants after the settling period, but would subsequently be removed by adding the fresh alum. The TSS, TCOD, and turbidity removal efficiencies were also enhanced when both the alum sludge and the fresh alum were employed. The TCOD removal efficiency over 80% has been accomplished, which has never fulfilled by using the fresh alum alone. It is concluded that the alum sludge could be reused for the treatment of industrial wastewater generated by the consumer products industry.展开更多
A widespread use of acrylamide, probably a neurotoxicant and carcinogen, in various industrial processes has led to environmental contamination. Fortunately, some microorganisms are able to derive energy from acrylami...A widespread use of acrylamide, probably a neurotoxicant and carcinogen, in various industrial processes has led to environmental contamination. Fortunately, some microorganisms are able to derive energy from acrylamide. In the present work, we reported the isolation and characterization of a novel acrylamide-degrading bacterium from domestic wastewater in Chonburi, Thailand. The strain grew well in the presence of acrylamide as 0.5% （W/V）, at pH 6.0 to 9,0 and 25℃. Identification based on biochemical characteristics and 16S rRNA gene sequence identified the strain as Enterobacter aerogenes. Degradation of acrylamide to acrylic acid started in the late logarithmic growth phase as a biomass-dependent pattern. Specificity of cell-free supernatant towards amides completely degraded butyramide and urea and 86% of lactamide. Moderate degradation took place in other amides with that by formanaide 〉 benzamide 〉 acetamide 〉 cyanoacetamide 〉 propionamide. No degradation was detected in the reactions of N,N-methylene bisacrylamide, sodium azide, thioacetamide, and iodoacetamide. These results highlighted the potential of this bacterium in the cleanup of acrylamide/amide in the environment.展开更多
Owing to the high viscosity of sodium silicate solution, fly ash geopolymer has the problems of low workability and rapid setting time. Therefore, the effect of chemical admixtures on the properties of fly ash geopoly...Owing to the high viscosity of sodium silicate solution, fly ash geopolymer has the problems of low workability and rapid setting time. Therefore, the effect of chemical admixtures on the properties of fly ash geopolymer was studied to overcome the rapid set of the geo-polymer in this paper. High-calcium fly ash and alkaline solution were used as starting materials to synthesize the geopolymer. Calcium chloride, calcium sulfate, sodium sulfate, and sucrose at dosages of 1wt% and 2wt% of fly ash were selected as admixtures based on concrete knowledge to improve the properties of the geopolymer. The setting time, compressive strength, and degree of reaction were recorded, and the microstructure was examined. The results show that calcium chloride significantly shortens both the initial and final setting times of the geopolymer paste. In addition, sucrose also delays the final setting time significantly. The degrees of reaction of fly ash in the geopolymer paste with the admixtures are all higher than those of the control paste. This contributes to the obvious increases in compressive strength.展开更多
A monoclonal antibody specific to sea bass(Lates calcarifer) vitellogenin(VTG) was developed,for use as a tool for monitoring endocrine disrupting chemicals(EDCs). VTG was induced in sea bass by intramuscular injectio...A monoclonal antibody specific to sea bass(Lates calcarifer) vitellogenin(VTG) was developed,for use as a tool for monitoring endocrine disrupting chemicals(EDCs). VTG was induced in sea bass by intramuscular injection of 17β-estradiol(E_2: 2 mg/kg) every three days. Blood was collected three days after the last injection. Plasma VTG was then purified by chromatography in hydroxyapatite and a sephacryl-S300 column. Characterizations of purified VTG were done by phospholipoglycoprotein staining on a native-PAGE with confirmation by mass spectrometry(LC-MS/MS). Antibody was raised in mice by injection of purified VTG. After monoclonal antibody production, the hybridoma clone No. 41(MAb-sea bass VTG 41)was selected and developed for quantification of VTG by competitive enzyme-linked immunosorbent assay(ELISA). The ELISA method was sensitive with a detection limit of VTG 40 ng/mL. MAb-sea bass VTG 41 was specific to VTG from E_2-treated sea bass and others EDCs(Nonylphenol, Benzo[a]pyrene and CdCl_2). Moreover, cross-reactivity was also found in E_2-treated coral grouper(Epinephelus corallicola). The ELISA method obtained from this work can be further applied for the assessment of EDCs in Thailand and Southeast Asia's aquatic environment.展开更多
BACKGROUND The exact mechanism of proton pump inhibitors(PPIs)-induced hypomagnesemia(PPIH) is largely unknown. Previous studies proposed that PPIH is a consequence of intestinal Mg2+ malabsorption. However, the mecha...BACKGROUND The exact mechanism of proton pump inhibitors(PPIs)-induced hypomagnesemia(PPIH) is largely unknown. Previous studies proposed that PPIH is a consequence of intestinal Mg2+ malabsorption. However, the mechanism of PPIs-suppressed intestinal Mg2+ absorption is under debate.AIM To investigate the effect of 12-wk and 24-wk omeprazole injection on the total,transcellular, and paracellular Mg2+ absorption in the duodenum, jejunum, ileum,and colon of male Sprague-Dawley rats.METHODS The rats received 20 mg/kg·d subcutaneous omeprazole injection for 12 or 24 wk.Plasma and urinary Mg2+, Ca2+, and PO43-levels were measured. The plasma concentrations of 1α,25-dihydroxyvitamin D3(1α,25(OH)2D3), parathyroid hormone(PTH), fibroblast growth factor 23(FGF-23), epidermal growth factor(EGF), and insulin were also observed. The duodenum, jejunum, ileum, and colon of each rat were mounted onto individual modified Using chamber setups to study the rates of total, transcellular, and paracellular Mg2+ absorption simultaneously. The expression of transient receptor potential melastatin 6(TRPM6) and cyclin M4(CNNM4) in the entire intestinal tract was also measured.RESULTS Single-dose omeprazole injection significantly increased the intraluminal p H of the stomach, duodenum, and jejunum. Omeprazole injection for 12 and 24 wk induced hypomagnesemia with reduced urinary Mg2+ excretion. The plasma Ca2+ was normal but the urinary Ca2+ excretion was reduced in rats with PPIH. The plasma and urinary PO43-levels increased in PPIH rats. The levels of1α,25(OH)2D3 and FGF-23 increased, whereas that of plasma EGF decreased in the omeprazole-treated rats. The rates of the total, transcellular, and paracellular Mg2+ absorption was significantly lower in the duodenum, jejunum, ileum, and colon of the rats with PPIH than in those of the control rats. The percent suppression of Mg2+ absorption in the duodenum, jejunum, ileum, and colon of the rats with PPIH compared with the control rats was 81.86%, 70.59%, 69.45%,and 39.25%, respectively. Compared with the control rats, the rats with PPIH had significantly higher TRPM6 and CNNM4 expression levels throughout the intestinal tract.CONCLUSION Intestinal Mg2+ malabsorption was observed throughout the intestinal tract of rats with PPIH. PPIs mainly suppressed small intestinal Mg2+ absorption. Omeprazole exerted no effect on the intraluminal acidic pH in the colon. Thus, the lowest percent suppression of total Mg2+ absorption was found in the colon. The expression levels of TRPM6 and CNNM4 increased, indicating the presence of a compensatory response to Mg2+ malabsorption in rats with PPIH. Therefore, the small intestine is an appropriate segment that should be modulated to counteract PPIH.展开更多
AIM:To elucidate the effect and underlying mechanisms of omeprazole action on Mg 2+ transport across the intestinal epithelium.METHODS:Caco-2 monolayers were cultured in various dose omeprazole-containing media for 14...AIM:To elucidate the effect and underlying mechanisms of omeprazole action on Mg 2+ transport across the intestinal epithelium.METHODS:Caco-2 monolayers were cultured in various dose omeprazole-containing media for 14 or 21 d before being inserted into a modified Ussing chamber apparatus to investigate the bi-directional Mg 2+ transport and electrical parameters.Paracellular permeability of the monolayer was also observed by the dilution potential technique and a cation permeability study.An Arrhenius plot was performed to elucidate the activation energy of passive Mg 2+ transport across the Caco-2 monolayers.RESULTS:Both apical to basolateral and basolateral to apical passive Mg 2+ fluxes of omeprazole-treated epithelium were decreased in a dose-and time-dependent manner.Omeprazole also decreased the paracellular cation selectivity and changed the paracellular selective permeability profile of Caco-2 epithelium to Li +,Na +,K +,Rb +,and Cs + from seriesⅦto seriesⅥof the Eisenman sequence.The Arrhenius plot revealed the higher activation energy for passive Mg 2+ transport in omeprazoletreated epithelium than that of control epithelium,indicating that omeprazole affected the paracellular channel of Caco-2 epithelium in such a way that Mg 2+ movement was impeded.CONCLUSION:Omeprazole decreased paracellular cation permeability and increased the activation energy for passive Mg 2+ transport of Caco-2 monolayers that led to the suppression of passive Mg 2+ absorption.展开更多
In this study, we developed a polymeric nanofiber patch(PNP) for topical disease treatment using electrohydrodynamic atomization(EHDA). The nanofibers were prepared using various concentrations of polyvinyl alcohol(PV...In this study, we developed a polymeric nanofiber patch(PNP) for topical disease treatment using electrohydrodynamic atomization(EHDA). The nanofibers were prepared using various concentrations of polyvinyl alcohol(PVA) and tamarind seed gum and loaded with clindamycin HCl as a model drug. The precursor polymer solutions were sprayed using the EHDA technique; the EHDA processing parameters were optimized to obtain blank and drug-loaded PNPs. The skin adherence, translucence, and ventilation properties of the prepared PNPs indicated that they are appropriate for topical application. The conductivity of the polymer solution increased with increasing PVA and clindamycin concentrations, and increasing the PVA concentration enhanced the solution viscosity. Based on scanning electron microscopy analysis, the PVA concentration had a pronounced effect on the morphology of the sprayed product. Nanofibers were fabricated successfully when the solution PVA concentration was 10%, 13%, or 15%(w/v). The applied voltage significantly affected the diameters of the prepared nanofibers, and the minimum nanofiber diameter was 163.86 nm. Differential scanning calorimetry and X-ray diffraction analyses indicated that the modeldrug was dispersed in PVA in an amorphous form. The PNP prepared with a PVA:gum ratio of 9:1 absorbed water better than the PVA-only PNP and the PNP with a PVA:gum ratio of 9.5:0.5. Moreover, the PNPs loaded with clindamycin at concentrations of 1%–3% prohibited the growth of Staphylococcus aureus more effectively than clindamycin gel, a commercially available product.展开更多
Most seed gums have been widely used in oral and topical pharmaceutical formulations, cosmetics, and food products because of their hydrophilic properties. Gums from Tamariudus indica and Cassia fistula seeds were che...Most seed gums have been widely used in oral and topical pharmaceutical formulations, cosmetics, and food products because of their hydrophilic properties. Gums from Tamariudus indica and Cassia fistula seeds were chemically modified by carboxymethylation to improve their functionalities. The objective of the present study was to characterize and evaluate crude and carboxymethylated gums from T. indica and C. fistula seeds to achieve the controlled-release of diclofenac sodium(DS) in matrix tablet form. Both crude and carboxymethylated gums were characterized by Fourier transform infrared spectroscopy, Xray diffraction, and scanning electron microscopy. The results revealed that the gums were successfully modified by carboxymethylation and that the modified gums were amorphous in structure and had better flow properties. The carboxymethylated gums from both plant seeds did not exhibit cytotoxicity at concentrations lower than 0.5 mg/ml. All gum samples used as polymeric controlled-release agents were formulated into DS matrix tablets. Hardness and thickness tests were conducted as in-process tests. Drug content estimation and in vitro drug release studies were carried out to evaluate the matrix tablets. Increasing the concentration of gums increased compression time and hardness while it reduced the thickness. Furthermore, the results fitted well with the Korsmeyer–Peppas model. Moreover, the DS tablets were found to release the drug by super case II transport(relaxation). In summary, the carboxymethylated gum from T. indica and C. fistula seeds is an excellent, naturally sourced gum with high physicochemical and functional qualities, and can potentially be used in pharmaceutical applications as a disintegrant, diluent, and drug release-controlling agent.展开更多
Tamarind(Tamarindus indica Linn.)is a topical plant that is generally found and planted in Thailand.Application of tamarind seed gum can increase the value of tamarind and minimize the industrial waste[1].Tamarind see...Tamarind(Tamarindus indica Linn.)is a topical plant that is generally found and planted in Thailand.Application of tamarind seed gum can increase the value of tamarind and minimize the industrial waste[1].Tamarind seed gum powder offers high viscosity solution.Therefore,researchers are interested in developing tamarind seed gum as binder in formulation of diclofenac sodium tablet,prepared by dry granulation method.展开更多
基金funded by Faculty of Engineering,Burapha University,grant number 003/2567.
文摘Herein,cure characteristics,morphology,and mechanical properties of natural rubber filled with activated carbon-based materials were investigated.Carbon-based materials were prepared from bagasse,coffee grounds and pineapple crowns by the pyrolysis method at temperatures in the range of 300℃.As-synthesized carbon materials were characterized by optical microscopy(OM),scanning electron microscopy(SEM),and Fourier-transform infrared spectroscopy(FTIR)to analyze size distribution,morphology,and functional groups,respectively.OM and SEM analysis revealed that particles,flakes,and a small quantity of fiber-like carbon were obtained using bagasse and pineapple crown as raw materials,while honeycomb-like carbon materials can be derived from coffee grounds.To investigate the mechanical properties,natural rubber was filled with carbon black and as-synthesized carbon materials by the internal mixing and compression molding process.Transmission electron microscopy(TEM)was utilized to characterize the dispersion of carbon materials in the rubber matrix.The results of tensile testing showed that the natural rubber mixed with as-synthesized carbon materials from pineapple crowns exhibited 54%and 74%improvement in the ultimate tensile strength and Young’s modulus,respectively,compared with natural rubber without filled carbon materials.The enhancement in mechanical properties by activated carbon materials derived from pineapple crowns can be attributed to the flake-and fiber-like structures and good dispersion of carbon materials in the rubber matrix.In addition,it is higher than that of rubber mixed with carbon black.The results demonstrated that as-synthesized carbon materials from pineapple crowns have the potential materials to substitute carbon black in the rubber compound industry.
基金grant Fundamental Fund of National Science Research and Innovation Fund(NSRF)via Burapha University of Thailand(Grant number 52/2024).
文摘Background:People working outdoors in the Map Ta Phut pollution control area of Thailand require comprehen-sive health monitoring.In the past,studies have been done on the health effects of pollutants.However,there are few studies on musculoskeletal disorders(MSDs),and Thailand is struggling to meet the Sustainable Development Goals.Methods:This cross-sectional study examines access to health services and factors affecting MSDs among outdoor pollution workers(OPWs).The sample group includes OPWs,including local fisherman,street vendors,public car drivers,and traffic police.We studied 50 people from each of these groups,for a total of 200 people.Data were analyzed with inferential statistics using Chi-square test,McNemar test,and Univariate logistic regression.Results:The OPWs reported experiencing significantly more total MSDs pain than they did in the past(P<0.05).Factors affecting current MSDs pain,including occupation and working days per week,were significant(P<0.05).The street vendor group and public car driver group had(odds ratio[OR]=2.253,95%confidence interval[CI]:1.101 to 5.019)and(OR=2.681,95%CI:1.191 to 6.032)times higher risks of MSDs pain,respectively.OPWs who work>5 days per week had a(OR=1.464,95%CI:1.093 to 2.704)times higher risk of MSDs pain.52.7%of OPWs with MSDs,pain(n=110)had received an annual health check-up.In the past year,50.9%had minor illnesses and 21.8%had severe illnesses.OPWs receiving free treatment and visiting health service stations for no cost comprised 77.3%and 51.8%,respectively.60.9%used their right to receive treatment with universal health insurance cards.Conclusions:The study indicates that occupational groups with MSDs pain problems should exercise this right,according to the worker protection law.Local health agencies should organize activities or create accessible media to promote preventive medicine services,as many OPWs believe that health services can only be accessed when illness occurs.
文摘This study examines how demographic factors shape engagement with virtual cultural communities and awareness of emerging economic models.Using survey data from 202 participants,descriptive statistics,independent samples t-tests,and One-way Analysis of Variance(ANOVA)with Least Significant Difference(LSD)post-hoc analyses revealed significant influences of age,education,and occupation.No significant gender differences were found.Individuals aged 26-45 demonstrated higher virtual community participation(F=3.439,p=0.01)and emerging model awareness(F=2.834,p=0.026)than younger cohorts(18-25 years and below).Respondents with postgraduate education exhibited superior understanding of emerging economic models(F=3.296,p=0.022)and their nexus with cultural values(F=6.196,p<0.001)compared to those with lower educational attainment.Significant occupational variations existed in virtual participation(F=4.001,p=0.008)and economic model awareness(F=5.611,p=0.001),with enterprise employees and civil servants scoring higher than students and freelancers.These findings underscore the critical roles of life stage,educational investment,and professional context in shaping digital cultural behaviors and economic cognition,offering valuable insights for platform design,educational strategies,and policy development in the digital cultural economy.
文摘Globalization triggers interactive effects between educational inequality and economic development differences,which are influenced by complex dynamics and feedback mechanisms.This paper uses a comprehensive literature review and qualitative case analysis method to deeply explore the status,impact and joint feedback cycles of this phenomenon,focusing on California and Bangalore.The results reveal its interactive driving forces and provide important perspectives for understanding this issue.At the same time,the study proposes forward-looking policy recommendations and emphasizes the need for further exploration,especially around the role of cultural factors in curbing global inequality.
文摘ESG(Environment,Society,Governance)management is being applied in the world’s industries,including ports,to enhance sustainable development.For the port sector,the world’s ports have increasingly adopted policies to drive ESG projects in alignment with sustainable port development goals.This research aims to identify port sustainability indicators and analyze the interrelationship among ESG dimensions related to the port environment,port society,and port governance.Qualitative and quantitative methods are employed to identify port management indicators that affect the operation of cargo ports,utilizing a systematic document analysis tool.A wide range of academic and institutional sources was reviewed to ensure the comprehensive identification of relevant indicators.The finding indicates that twenty-nine port sustainability indicators affect port sustainability management,including the nine indicators of environmental port,the nine indicators of social port,and the eleven indicators of governance port.These critical indicators reflect both global best practices and context-specific needs,and they play a central role in managing the port’s sustainability and guiding control strategies for improving competitiveness and compliance with international standards.Based on these findings,a port sustainability framework was developed as a practical operational tool to evaluate port performance,guide decision-making,and align port operations with Thailand’s national policies and global sustainability standards.This framework also provides a foundation for future empirical validation and offers practical implications for policymakers and port stakeholders.
基金funded by Burapha University,grant number SDG 4/2568.
文摘Nitrogen-doped activated carbon(N-AC)was successfully prepared by KOH-activation and nitrogen doping using ammonia(NH3)heat treatment.Coconut shell-derived activated carbon(AC)was heat-treated under NH3 gas in the temperature range of 700℃-900℃.Likewise,the mixture of potassium hydroxide(KOH)and AC was heated at 800℃,followed by heat treatment underNH3 gas at 800℃(hereafter referred to asKOH-N-AC800).Scanning electron microscopy(SEM),Raman spectroscopy,X-ray photoelectron spectroscopy(XPS)and Brunauer-Emmett-Teller(BET)method were utilized to analyze morphology,crystallinity,chemical bonding,chemical composition and surface area.The surface area and porosity of N-AC increased with increasing NH3 heat treatment.Similarly,the nitrogen content in the N-AC increased from 3.23%to 4.84 at%when the NH3 heat treatment was raised from 700℃ to 800℃.However,the nitrogen content of N-AC decreased to 3.40 at% after using NH3 heat treatment at 900℃.The nitrogen content of KOH-N-AC800 is 5.43 at%.KOH-N-AC800 and N-AC800 exhibited improvements of 33.66% and 26.24%,respectively,in CO_(2) adsorption compared with AC.The enhancement of CO_(2) adsorption of KOH-N-AC800 is attributed to the synergic effect of the nitrogen doping,high surface area,and porosity.The results exhibited that nitrogen sites on the surface play a more significant role in CO_(2) adsorption than surface area and porosity.This work proposes the potential synergistic effect of KOH-activation and nitrogen doping for enhancing the CO_(2) adsorption capacity of activated carbon.
基金supported by the Basic Research Fund from Thailand Science Research and Innovation through Sisaket Rajabhat University(grant number:FF.13/2564)。
文摘Objective:The present study investigated the cytoprotective effects of a Pogonatherum paniceum extract prepared with 80%ethanol(PPE)using synchrotron radiation-based Fourier transform infrared(SR-FTIR)microspectroscopy and determined its phytochemical profile.Methods:The volatile and polyphenolic compounds in PPE were characterized using gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry,respectively.The antioxidant capacity of PPE was evaluated using chemical and cell-based assays.The SR-FTIR microspectroscopy was performed to evaluate the cytoprotective effect of PPE by identifying changes in macromolecule composition in tert-butyl hydroperoxide(t BuOOH)-induced oxidative damage in RAW264.7 cells.Results:A total of 48 volatile compounds and 28 polyphenol components were found in PPE.PPE exhibited a high potential for antioxidant activity by scavenging the intracellular reactive oxygen species in t Bu OOH-induced oxidative damage in RAW264.7 cells.PPE treatment also significantly protected RAW264.7 cells against t BuOOH-induced toxicity and restored cell viability.The SR-FTIR analysis revealed that t BuOOH increased the lipid and ester lipid content in RAW264.7 cells.The PPE exerted a cytoprotective effect by decreasing the levels of lipid and ester lipid compounds that had been elevated by t BuOOH in RAW264.7 cells.These findings indicate that PPE has cytoprotective potential due to its ability to inhibit endogenous reactive oxygen species.Conclusion:This study extends the current knowledge on the phytochemistry of PPE and its antioxidant and cytoprotective effects.These findings support the use of SR-FTIR microspectroscopy to determine the cytoprotective effects of natural products.PPE extract may be a candidate compound for new therapeutics and nutraceuticals that target the prevention of oxidative stress-associated diseases.
基金Supported by Burapha University,Thailand Science Research and Innovation,and National Science Research and Innovation Fund,No.53/2567.
文摘BACKGROUND Magnesium(Mg^(2+))plays a fundamental role in numerous cellular processes,including enzymatic reactions,DNA replication,oxidative stress response,and cytoskeletal dynamics.In fact,dysregulation of Mg^(2+)homeostasis has been increasingly associated with the development and progression of cancer,particularly colorectal cancer(CRC).Transient receptor potential melastatin(TRPM)channels,especially TRPM6 and TRPM7,are essential regulators of epithelial Mg^(2+)influx.While TRPM7 promotes CRC progression,the role of TRPM6 and TRPM6/7 channels remains unclear.AIM To investigate the role of membrane-localized TRPM6 and TRPM6/7 channels in Mg^(2+)influx,spheroid(SP)formation,stemness,and migration.METHODS We used parental and SP-derived HT-29 cells at comparable passages as in vitro models.Mass spectrometry confirmed full-length sequences,phosphorylation,and methionine oxidation of TRPM6 and TRPM7.Mg^(2+)influx,total and free Mg^(2+)levels were measured by fluorescence imaging and biochemical assays.TRPM6/TRPM7 expression and markers were analyzed by western blot.Func-tional assays,including secondary SP formation and wound healing,assessed stemness and migration.Cells were treated with Mg^(2+)transport inhibitors:Co(III)hexamine,2-aminoethyl diphenylborinate(TRPM6/7 blocker),and Mesendogen(TRPM6 inhibitor).RESULTS The expression of membrane-bound TRPM6,TRPM7,and TRPM6/7 was significantly higher in SP cells than in parental cells.Mass spectrometric analysis confirmed the presence of full-length TRPM6 and TRPM7 with increased phosphorylation and oxidation in SP cells.Enhanced Mg^(2+)influx and total intracellular Mg^(2+)levels were observed in SP cells.Free ionized intracellular Mg^(2+)levels remained comparable across all experimental groups.Pharmacological inhibition of TRPM6 and TRPM6/7 significantly reduced Mg^(2+)influx,decreased total Mg^(2+)content,compromised CRC SP stability,abolished cancer stem-like properties,impaired cell migration,and downregulated pro-tumorigenic markers,including Nanog,cyclooxygenase-2,and matrix metalloproteinase-9.CONCLUSION Membrane-localized TRPM6 and TRPM6/7 channels regulate Mg^(2+)influx and promote CRC stemness,SP stability,and migration,highlighting their potential as therapeutic targets to inhibit CRC progression and metastasis.
基金supported by the Faculty of Nursing Burapha University(No.19/2561).
文摘Objectives: To determine the level of adversity quotient and its associations with rumination, optimism, and authoritative parenting style in junior high school students. Adversity quotient is the adaptation ability to overcome problems and obstacles with a positive attitude. Early adolescence, as a critical stage of life, needs to cultivate adversity quotient. However, adversity quotient and its influencing factors in adolescents have not been fully understood. Methods: A total of 129 students from 3 junior high schools (grade 7-9) in Chon Buri province, Thailand, were randomly recruited to participate in this correlational research study. They completed 5 questionnaires including the demographic form, adversity quotient assessment, authoritative parenting style, optimism, and rumination. Descriptive statistics and Pearson’s correlation coefficient were used for data analysis. Results: Adversity quotient was at a moderate level with a mean of 138.78 (standard deviation [SD] = 21.25). Adversity quotient was significantly, positively correlated with authoritative parenting at the highest level (r = 0.60), followed by optimism at a moderate level (r = 0.38), and rumination at a low level (r = 0.25, P < 0.01). Conclusions: All parties responsible for early adolescents’ development should enhance the adversity quotient to a higher level through campaigns promoting authoritative parenting and optimism. An unexpected positive correlation between adversity quotient and rumination warrants cautious interpretation and more studies.
文摘Osteoporosis,a global age-related health problem in both male and female elderly,insidiously deteriorates the microstructure of bone,particularly at trabecular sites,such as vertebrae,ribs and hips,culminating in fragility fractures,pain and disability.Although osteoporosis is normally associated with senescence and estrogen deficiency,diabetes mellitus(DM),especially type 1 DM,also contributes to and/or aggravates bone loss in osteoporotic patients.This topic highlight article focuses on DM-induced osteoporosis and DM/ osteoporosis comorbidity,covering alterations in bone metabolism as well as factors regulating bone growth under diabetic conditions including,insulin,insulin-like growth factor-1 and angiogenesis.Cellular and molecular mechanisms of DM-related bone loss are also discussed.This information provides a foundation for the better understanding of diabetic complications and for development of early screening and prevention of osteoporosis in diabetic patients.
基金Supported by Grants from the Cluster and Program Management Office(CPMO),National Science and Technology Development Agency(P-11-00639)the Thailand Research Fund(TRF)-Mahidol University through the TRF Senior Research Scholar Grant(RTA5780001 to NC)+2 种基金the Faculty of Allied Health Sciences,Burapha University and Thailand Research Fund through TRF Research Career Development Grant(RSA5780041 to KW)the Research and Development Fund Burapha University(05/2557 to KW)the Faculty of Allied Health Sciences,Burapha University Research Grant of Fiscal Year 2015(AHS05/2558 to KW)
文摘It was previously understood that body weight gain and obesity observed in type 2 diabetes mellitus(T2DM) could be beneficial since body weight increase elevated bone mineral density and thus helped maintain the skeletal framework.However,a number of recent findings in humans and rodents have revealed that T2 DM is not only associated with trabecular defects but also increases cortical porosity,and compromised bone cell function and bone mechanical properties.Hyperglycemia and insulin resistance in T2 DM may further induce osteoblast apoptosis and uncoupling bone turnover.Prolonged accumulation of advanced glycation end products and diminished activity of lysyl oxidase,an essential enzyme for collagen cross-link,can lead to structural abnormalities of bone collagen fibrils,brittle matrix,and fragility fractures.Our studies in T2 DM rats showed that dyslipidemia,which often occurs in T2 DM,could obscure the T2DM-associated changes in bone microstructure and osteopenia.Longitudinal bone growth regulated by the growth plate chondrocytes is also impaired by T2 DM since differentiation of growth plate chondrocytes is arrested and retained in the resting state while only a small number of cells undergo hypertrophic differentiation.Such a delayed chondrocyte differentiation may have also resulted from premature apoptosis of the growth plate chondrocytes.Nevertheless,the underlying cellular and molecular mechanisms of insulin resistance in osteoblasts,osteoclasts,osteocytes,and growth plate chondrocytes remain to be investigated.
基金the Research and Development Fund of the Faculty of Engineering,Burapha University(No.70/2551)for financial supports
文摘A coagulation-flocculation process is typically employed to treat the industrial wastewater generated by the consumer products industry manufacturing detergents, soaps, and others. The expenditure of chemicals including coagulants and chemicals for pH adjustment is costly for treating this wastewater. The objective of this study was to evaluate the feasibility of reusing the aluminum sulfate （alum） sludge as a coagulant or as a coagulation aid so that the fresh alum dosage can be minimized or the removal efficiency can be enhanced. The experiments were conducted in a jar-test apparatus simulating the coagulation-flocculation process for simultaneous removals of organic matters, anionic surfactants, suspended solids, and turbidity. At the optimum initial pH value of 10 and the fresh alum concentration of 400 mg/L, the total suspended solids （TSS）, total chemical oxygen demand （TCOD）, total anionic surfactants, and turbidity removal efficiencies were 71.5%, 76.4%, 95.4%, and 98.2%, respectively. The addition of alum sludge as a coagulant alone without any fresh alum addition could significantly remove the turbidity, TCOD, and anionic surfactants. The TSS was left in the supernatants after the settling period, but would subsequently be removed by adding the fresh alum. The TSS, TCOD, and turbidity removal efficiencies were also enhanced when both the alum sludge and the fresh alum were employed. The TCOD removal efficiency over 80% has been accomplished, which has never fulfilled by using the fresh alum alone. It is concluded that the alum sludge could be reused for the treatment of industrial wastewater generated by the consumer products industry.
基金Financial support was mainly provided to JittimaCharoenpanich from Center of Excellence on Environmental Health,Toxicology and Management of Chemicals(ETM-PERDO)partly from Faculty of Science,Burapha Universitya scholarship support to Kanokhathai Buranasilp from Center of Excellence for Innovation in Chemistry (PERCH-CIC),Commission on Higher Education,Ministry of Education
文摘A widespread use of acrylamide, probably a neurotoxicant and carcinogen, in various industrial processes has led to environmental contamination. Fortunately, some microorganisms are able to derive energy from acrylamide. In the present work, we reported the isolation and characterization of a novel acrylamide-degrading bacterium from domestic wastewater in Chonburi, Thailand. The strain grew well in the presence of acrylamide as 0.5% （W/V）, at pH 6.0 to 9,0 and 25℃. Identification based on biochemical characteristics and 16S rRNA gene sequence identified the strain as Enterobacter aerogenes. Degradation of acrylamide to acrylic acid started in the late logarithmic growth phase as a biomass-dependent pattern. Specificity of cell-free supernatant towards amides completely degraded butyramide and urea and 86% of lactamide. Moderate degradation took place in other amides with that by formanaide 〉 benzamide 〉 acetamide 〉 cyanoacetamide 〉 propionamide. No degradation was detected in the reactions of N,N-methylene bisacrylamide, sodium azide, thioacetamide, and iodoacetamide. These results highlighted the potential of this bacterium in the cleanup of acrylamide/amide in the environment.
文摘Owing to the high viscosity of sodium silicate solution, fly ash geopolymer has the problems of low workability and rapid setting time. Therefore, the effect of chemical admixtures on the properties of fly ash geopolymer was studied to overcome the rapid set of the geo-polymer in this paper. High-calcium fly ash and alkaline solution were used as starting materials to synthesize the geopolymer. Calcium chloride, calcium sulfate, sodium sulfate, and sucrose at dosages of 1wt% and 2wt% of fly ash were selected as admixtures based on concrete knowledge to improve the properties of the geopolymer. The setting time, compressive strength, and degree of reaction were recorded, and the microstructure was examined. The results show that calcium chloride significantly shortens both the initial and final setting times of the geopolymer paste. In addition, sucrose also delays the final setting time significantly. The degrees of reaction of fly ash in the geopolymer paste with the admixtures are all higher than those of the control paste. This contributes to the obvious increases in compressive strength.
基金financially supported by the Research grant of Burapha University through the National Research Council of Thailand (No.79/2558)Graduate study of faculty of Science Burapha University
文摘A monoclonal antibody specific to sea bass(Lates calcarifer) vitellogenin(VTG) was developed,for use as a tool for monitoring endocrine disrupting chemicals(EDCs). VTG was induced in sea bass by intramuscular injection of 17β-estradiol(E_2: 2 mg/kg) every three days. Blood was collected three days after the last injection. Plasma VTG was then purified by chromatography in hydroxyapatite and a sephacryl-S300 column. Characterizations of purified VTG were done by phospholipoglycoprotein staining on a native-PAGE with confirmation by mass spectrometry(LC-MS/MS). Antibody was raised in mice by injection of purified VTG. After monoclonal antibody production, the hybridoma clone No. 41(MAb-sea bass VTG 41)was selected and developed for quantification of VTG by competitive enzyme-linked immunosorbent assay(ELISA). The ELISA method was sensitive with a detection limit of VTG 40 ng/mL. MAb-sea bass VTG 41 was specific to VTG from E_2-treated sea bass and others EDCs(Nonylphenol, Benzo[a]pyrene and CdCl_2). Moreover, cross-reactivity was also found in E_2-treated coral grouper(Epinephelus corallicola). The ELISA method obtained from this work can be further applied for the assessment of EDCs in Thailand and Southeast Asia's aquatic environment.
基金Burapha University through National Research Council of Thailand,No.15/2562。
文摘BACKGROUND The exact mechanism of proton pump inhibitors(PPIs)-induced hypomagnesemia(PPIH) is largely unknown. Previous studies proposed that PPIH is a consequence of intestinal Mg2+ malabsorption. However, the mechanism of PPIs-suppressed intestinal Mg2+ absorption is under debate.AIM To investigate the effect of 12-wk and 24-wk omeprazole injection on the total,transcellular, and paracellular Mg2+ absorption in the duodenum, jejunum, ileum,and colon of male Sprague-Dawley rats.METHODS The rats received 20 mg/kg·d subcutaneous omeprazole injection for 12 or 24 wk.Plasma and urinary Mg2+, Ca2+, and PO43-levels were measured. The plasma concentrations of 1α,25-dihydroxyvitamin D3(1α,25(OH)2D3), parathyroid hormone(PTH), fibroblast growth factor 23(FGF-23), epidermal growth factor(EGF), and insulin were also observed. The duodenum, jejunum, ileum, and colon of each rat were mounted onto individual modified Using chamber setups to study the rates of total, transcellular, and paracellular Mg2+ absorption simultaneously. The expression of transient receptor potential melastatin 6(TRPM6) and cyclin M4(CNNM4) in the entire intestinal tract was also measured.RESULTS Single-dose omeprazole injection significantly increased the intraluminal p H of the stomach, duodenum, and jejunum. Omeprazole injection for 12 and 24 wk induced hypomagnesemia with reduced urinary Mg2+ excretion. The plasma Ca2+ was normal but the urinary Ca2+ excretion was reduced in rats with PPIH. The plasma and urinary PO43-levels increased in PPIH rats. The levels of1α,25(OH)2D3 and FGF-23 increased, whereas that of plasma EGF decreased in the omeprazole-treated rats. The rates of the total, transcellular, and paracellular Mg2+ absorption was significantly lower in the duodenum, jejunum, ileum, and colon of the rats with PPIH than in those of the control rats. The percent suppression of Mg2+ absorption in the duodenum, jejunum, ileum, and colon of the rats with PPIH compared with the control rats was 81.86%, 70.59%, 69.45%,and 39.25%, respectively. Compared with the control rats, the rats with PPIH had significantly higher TRPM6 and CNNM4 expression levels throughout the intestinal tract.CONCLUSION Intestinal Mg2+ malabsorption was observed throughout the intestinal tract of rats with PPIH. PPIs mainly suppressed small intestinal Mg2+ absorption. Omeprazole exerted no effect on the intraluminal acidic pH in the colon. Thus, the lowest percent suppression of total Mg2+ absorption was found in the colon. The expression levels of TRPM6 and CNNM4 increased, indicating the presence of a compensatory response to Mg2+ malabsorption in rats with PPIH. Therefore, the small intestine is an appropriate segment that should be modulated to counteract PPIH.
基金Supported by The Thailand Research Fund(to Thongon N),No.MRG5380003
文摘AIM:To elucidate the effect and underlying mechanisms of omeprazole action on Mg 2+ transport across the intestinal epithelium.METHODS:Caco-2 monolayers were cultured in various dose omeprazole-containing media for 14 or 21 d before being inserted into a modified Ussing chamber apparatus to investigate the bi-directional Mg 2+ transport and electrical parameters.Paracellular permeability of the monolayer was also observed by the dilution potential technique and a cation permeability study.An Arrhenius plot was performed to elucidate the activation energy of passive Mg 2+ transport across the Caco-2 monolayers.RESULTS:Both apical to basolateral and basolateral to apical passive Mg 2+ fluxes of omeprazole-treated epithelium were decreased in a dose-and time-dependent manner.Omeprazole also decreased the paracellular cation selectivity and changed the paracellular selective permeability profile of Caco-2 epithelium to Li +,Na +,K +,Rb +,and Cs + from seriesⅦto seriesⅥof the Eisenman sequence.The Arrhenius plot revealed the higher activation energy for passive Mg 2+ transport in omeprazoletreated epithelium than that of control epithelium,indicating that omeprazole affected the paracellular channel of Caco-2 epithelium in such a way that Mg 2+ movement was impeded.CONCLUSION:Omeprazole decreased paracellular cation permeability and increased the activation energy for passive Mg 2+ transport of Caco-2 monolayers that led to the suppression of passive Mg 2+ absorption.
基金the Faculty of Pharmaceutical Sci-ences,Burapha University for financial support(grant num-bers 9/2558)
文摘In this study, we developed a polymeric nanofiber patch(PNP) for topical disease treatment using electrohydrodynamic atomization(EHDA). The nanofibers were prepared using various concentrations of polyvinyl alcohol(PVA) and tamarind seed gum and loaded with clindamycin HCl as a model drug. The precursor polymer solutions were sprayed using the EHDA technique; the EHDA processing parameters were optimized to obtain blank and drug-loaded PNPs. The skin adherence, translucence, and ventilation properties of the prepared PNPs indicated that they are appropriate for topical application. The conductivity of the polymer solution increased with increasing PVA and clindamycin concentrations, and increasing the PVA concentration enhanced the solution viscosity. Based on scanning electron microscopy analysis, the PVA concentration had a pronounced effect on the morphology of the sprayed product. Nanofibers were fabricated successfully when the solution PVA concentration was 10%, 13%, or 15%(w/v). The applied voltage significantly affected the diameters of the prepared nanofibers, and the minimum nanofiber diameter was 163.86 nm. Differential scanning calorimetry and X-ray diffraction analyses indicated that the modeldrug was dispersed in PVA in an amorphous form. The PNP prepared with a PVA:gum ratio of 9:1 absorbed water better than the PVA-only PNP and the PNP with a PVA:gum ratio of 9.5:0.5. Moreover, the PNPs loaded with clindamycin at concentrations of 1%–3% prohibited the growth of Staphylococcus aureus more effectively than clindamycin gel, a commercially available product.
基金the Higher Education Research Promotion-National Research Universities(HERP-NRU)for financial support under grant no.2559A10862013
文摘Most seed gums have been widely used in oral and topical pharmaceutical formulations, cosmetics, and food products because of their hydrophilic properties. Gums from Tamariudus indica and Cassia fistula seeds were chemically modified by carboxymethylation to improve their functionalities. The objective of the present study was to characterize and evaluate crude and carboxymethylated gums from T. indica and C. fistula seeds to achieve the controlled-release of diclofenac sodium(DS) in matrix tablet form. Both crude and carboxymethylated gums were characterized by Fourier transform infrared spectroscopy, Xray diffraction, and scanning electron microscopy. The results revealed that the gums were successfully modified by carboxymethylation and that the modified gums were amorphous in structure and had better flow properties. The carboxymethylated gums from both plant seeds did not exhibit cytotoxicity at concentrations lower than 0.5 mg/ml. All gum samples used as polymeric controlled-release agents were formulated into DS matrix tablets. Hardness and thickness tests were conducted as in-process tests. Drug content estimation and in vitro drug release studies were carried out to evaluate the matrix tablets. Increasing the concentration of gums increased compression time and hardness while it reduced the thickness. Furthermore, the results fitted well with the Korsmeyer–Peppas model. Moreover, the DS tablets were found to release the drug by super case II transport(relaxation). In summary, the carboxymethylated gum from T. indica and C. fistula seeds is an excellent, naturally sourced gum with high physicochemical and functional qualities, and can potentially be used in pharmaceutical applications as a disintegrant, diluent, and drug release-controlling agent.
文摘Tamarind(Tamarindus indica Linn.)is a topical plant that is generally found and planted in Thailand.Application of tamarind seed gum can increase the value of tamarind and minimize the industrial waste[1].Tamarind seed gum powder offers high viscosity solution.Therefore,researchers are interested in developing tamarind seed gum as binder in formulation of diclofenac sodium tablet,prepared by dry granulation method.
