BACKGROUND Schizophrenia(SZ),a chronic and widespread brain disorder,presents with complex etiology and pathogenesis that remain inadequately understood.Despite the absence of a universally recognized endophenotype,pe...BACKGROUND Schizophrenia(SZ),a chronic and widespread brain disorder,presents with complex etiology and pathogenesis that remain inadequately understood.Despite the absence of a universally recognized endophenotype,peripheral blood mononuclear cells(PBMCs)serve as a robust model for investigating intracellular alterations linked to SZ.AIM To preliminarily investigate potential pathogenic mechanisms and identify novel biomarkers for SZ.METHODS PBMCs from SZ patients were subjected to integrative transcriptomic and proteomic analyses to uncover differentially expressed genes(DEGs)and differentially expressed proteins while mapping putative disease-associated signaling pathways.Key findings were validated using western blot(WB)and real-time fluorescence quantitative PCR(RT-qPCR).RNAi-lentivirus was employed to transfect rat hippocampal CA1 neurons in vitro,with subsequent verification of target gene expression via RT-qPCR.The levels of neuronal conduction proteins,including calmodulin-dependent protein kinase II(caMKII),CREB,and BDNF,were assessed through WB.Apoptosis was quantified by flow cytometry,while cell proliferation and viability were evaluated using the Cell Counting Kit-8 assay.RESULTS The integration of transcriptomic and proteomic analyses identified 6079 co-expressed genes,among which 25 DEGs were significantly altered between the SZ group and healthy controls.Notably,haptoglobin(HP),lactotransferrin(LTF),and SERPING1 exhibited marked upregulation.KEGG pathway enrichment analysis implicated neuroactive ligand-receptor interaction pathways in disease pathogenesis.Clinical sample validation demonstrated elevated protein and mRNA levels of HP,LTF,and SERPING1 in the SZ group compared to controls.WB analysis of all clinical samples further corroborated the significant upregulation of SERPING1.In hippocampal CA1 neurons transfected with lentivirus,reduced SERPING1 expression was accompanied by increased levels of CaMKII,CREB,and BDNF,enhanced cell viability,and reduced apoptosis.CONCLUSION SERPING1 may suppress neural cell proliferation in SZ patients via modulation of the CaMKII-CREB-BDNF signaling pathway.展开更多
Objective:To investigate the levels of immune function and inflammatory factors in children with mycoplasma pneumonia (MP) infection in different stages and its clinical significance. Methods: A total of 100 children ...Objective:To investigate the levels of immune function and inflammatory factors in children with mycoplasma pneumonia (MP) infection in different stages and its clinical significance. Methods: A total of 100 children with MPP hospitalized from September 2014 to May 2015 were selected as the observation group and divided into two groups of MPP acute period and recovery period on the basis of the infection degree, and 50 healthy children were selected as control group at the same time, detect the immunoglobulin (IgG, IgA, IgM) and complement (C3, C4), peripheral T lymphocyte subsets (CD3+, CD4+, CD8+) and inflammatory factors (IL-2, IL-6, TNF-α, IL-8, IL-10, IL-13) of three groups of infants.Results:immunoglobulin IgG levels of recovery period were significantly higher than that in acute stage and the control group, IgA levels of acute phase and recovery phase were significantly lower than the control group, IgM and complement C3 levels in acute stage were significantly higher than the control group, during the recovery period tended to be normal, complement C4 in acute stage levels were significantly higher than those in recovery period and both higher than the control group. The CD3+, CD4+ and CD4/CD8 ratio of MPP in acute stage and recovery phase were significantly lower than the control group, and these numerical indexes of the recovery period were significantly higher than the acute stage, CD8+ levels in acute stage were significantly higher than that in recovery period and both higher than control group. Compared with the control group ,The levels of inflammatory cytokines IL-2 in the acute phase and recovery phase were significantly decreased, and the recovery period was significantly higher than that in acute stage, the test results that the levels of IL-6, TNF-α, IL-8, IL-10 and IL-13 in acute phase and recovery phase were significantly higher than those in control group and the recovery period was significantly lower than that in the acute stage.Conclusion:The body humoral immune function and cellular immune function damage caused by MP infection in infants and young children could lead to the disorder of immune system and the abnormal expression of inflammatory cytokines. Therefore, Therefore, the real-time monitoring the dynamic level of the indicators can define the infants and young children's condition, improve the recovery rate, and have a certain guiding significance of the clinical diagnosis of infants and young children .展开更多
基金Supported by the Key R&D Projects of Hainan Province,No.ZDYF2022SHFZ295.
文摘BACKGROUND Schizophrenia(SZ),a chronic and widespread brain disorder,presents with complex etiology and pathogenesis that remain inadequately understood.Despite the absence of a universally recognized endophenotype,peripheral blood mononuclear cells(PBMCs)serve as a robust model for investigating intracellular alterations linked to SZ.AIM To preliminarily investigate potential pathogenic mechanisms and identify novel biomarkers for SZ.METHODS PBMCs from SZ patients were subjected to integrative transcriptomic and proteomic analyses to uncover differentially expressed genes(DEGs)and differentially expressed proteins while mapping putative disease-associated signaling pathways.Key findings were validated using western blot(WB)and real-time fluorescence quantitative PCR(RT-qPCR).RNAi-lentivirus was employed to transfect rat hippocampal CA1 neurons in vitro,with subsequent verification of target gene expression via RT-qPCR.The levels of neuronal conduction proteins,including calmodulin-dependent protein kinase II(caMKII),CREB,and BDNF,were assessed through WB.Apoptosis was quantified by flow cytometry,while cell proliferation and viability were evaluated using the Cell Counting Kit-8 assay.RESULTS The integration of transcriptomic and proteomic analyses identified 6079 co-expressed genes,among which 25 DEGs were significantly altered between the SZ group and healthy controls.Notably,haptoglobin(HP),lactotransferrin(LTF),and SERPING1 exhibited marked upregulation.KEGG pathway enrichment analysis implicated neuroactive ligand-receptor interaction pathways in disease pathogenesis.Clinical sample validation demonstrated elevated protein and mRNA levels of HP,LTF,and SERPING1 in the SZ group compared to controls.WB analysis of all clinical samples further corroborated the significant upregulation of SERPING1.In hippocampal CA1 neurons transfected with lentivirus,reduced SERPING1 expression was accompanied by increased levels of CaMKII,CREB,and BDNF,enhanced cell viability,and reduced apoptosis.CONCLUSION SERPING1 may suppress neural cell proliferation in SZ patients via modulation of the CaMKII-CREB-BDNF signaling pathway.
文摘Objective:To investigate the levels of immune function and inflammatory factors in children with mycoplasma pneumonia (MP) infection in different stages and its clinical significance. Methods: A total of 100 children with MPP hospitalized from September 2014 to May 2015 were selected as the observation group and divided into two groups of MPP acute period and recovery period on the basis of the infection degree, and 50 healthy children were selected as control group at the same time, detect the immunoglobulin (IgG, IgA, IgM) and complement (C3, C4), peripheral T lymphocyte subsets (CD3+, CD4+, CD8+) and inflammatory factors (IL-2, IL-6, TNF-α, IL-8, IL-10, IL-13) of three groups of infants.Results:immunoglobulin IgG levels of recovery period were significantly higher than that in acute stage and the control group, IgA levels of acute phase and recovery phase were significantly lower than the control group, IgM and complement C3 levels in acute stage were significantly higher than the control group, during the recovery period tended to be normal, complement C4 in acute stage levels were significantly higher than those in recovery period and both higher than the control group. The CD3+, CD4+ and CD4/CD8 ratio of MPP in acute stage and recovery phase were significantly lower than the control group, and these numerical indexes of the recovery period were significantly higher than the acute stage, CD8+ levels in acute stage were significantly higher than that in recovery period and both higher than control group. Compared with the control group ,The levels of inflammatory cytokines IL-2 in the acute phase and recovery phase were significantly decreased, and the recovery period was significantly higher than that in acute stage, the test results that the levels of IL-6, TNF-α, IL-8, IL-10 and IL-13 in acute phase and recovery phase were significantly higher than those in control group and the recovery period was significantly lower than that in the acute stage.Conclusion:The body humoral immune function and cellular immune function damage caused by MP infection in infants and young children could lead to the disorder of immune system and the abnormal expression of inflammatory cytokines. Therefore, Therefore, the real-time monitoring the dynamic level of the indicators can define the infants and young children's condition, improve the recovery rate, and have a certain guiding significance of the clinical diagnosis of infants and young children .
