Urbanization and environmental degradation have led to significant declines in water quality and aquatic ecosystem health,highlighting the urgent need for effective restoration efforts.This study applies an integrated...Urbanization and environmental degradation have led to significant declines in water quality and aquatic ecosystem health,highlighting the urgent need for effective restoration efforts.This study applies an integrated analysis approach to estimate the economic value and benefits of improvements in water quality and aquatic ecosystem services resulting from the Ecological Stream Restoration Project.Using survey data analyzed through the choice experiment(CE)method,we assessed respondents’preferences for various ecosystem services,including water-friendly services,ecological functions,water-level control,and water-quality purification.Three empirical analysis models—the Conditional Logit Model(CLM),Nested Logit Model(NL),and Error Component Logit Model(ECL)—were applied,with the ECL model identified as the most suitable for this study.From the physical impact assessment,we derived compensating variations to estimate the annual economic benefits of the project.The estimated annual economic value of water quality improvement due to the Anyangcheon Ecological Stream Restoration Project ranged from approximately KRW 10.54 billion to KRW 21.44 billion,while the economic value of aquatic ecosystem improvement was estimated to range from KRW 6.05 billion to KRW 12.30 billion annually.This study provides analytic framework that can inform future ecological restoration projects and sustainable water management policies.展开更多
This study examines the empirical feasibility of quantitatively integrating environmental value information into Strategic Environmental Assessment(SEA).An analytical framework was established to incorporate environme...This study examines the empirical feasibility of quantitatively integrating environmental value information into Strategic Environmental Assessment(SEA).An analytical framework was established to incorporate environmental cost estimates into the SEA process by utilizing ecosystem service unit values provided by the Environmental Valuation Information System(EVIS),a national platform developed to support the evaluation of policies and projects.The framework was applied to a case study involving a multipurpose rural water development project in South Korea.Ecosystem service losses resulting from the project were quantified using biophysical indicators,such as vegetation biomass,forest area,and hydrological functions,and subsequently monetized through the application of the market price method,replacement cost method,and contingent valuation method.The total annual environmental cost was estimated to be approximately KRW 56.18 billion,with the majority attributable to losses in forest conservation and climate regulation services.These findings demonstrate that quantified environmental data can serve as a robust basis for alternative comparison and site evaluation within SEA.The study provides empirical evidence supporting the advancement of SEA from a predominantly procedural tool focused on environmental protection to a more comprehensive sustainability assessment framework that integrates environmental,economic,and social considerations.Furthermore,the results suggest that EVIS-based quantitative information holds potential for broader application in other national evaluation systems,such as preliminary feasibility studies and regulatory impact assessments.展开更多
The electrocatalytic oxidation of glycerol toward formic acid is one of the most promising pathways for transformation and utilization of glycerol.Herein,a series of well-defined Ni_(n)(SR)_(2n) nanoclusters(n=4,5,6;d...The electrocatalytic oxidation of glycerol toward formic acid is one of the most promising pathways for transformation and utilization of glycerol.Herein,a series of well-defined Ni_(n)(SR)_(2n) nanoclusters(n=4,5,6;denoted as Ni NCs)were prepared for the electrocatalytic glycerol oxidation toward formic acid,in which Ni_(6)-PET-50CV afforded the most excellent electrocatalytic performance with a high formic acid selectivity of 93% and a high glycerol conversion of 86%.This was attributed to the lowest charge transfer impedance and the most rapid reaction kinetics.Combined electrochemical measurements and X-ray absorption fine structure measurements revealed that the structures of Ni NCs remained intact after CV scanning pretreatment and electrocatalysis via forming the Ni–O bond.Additionally,the kinetic studies and in-situ Fourier transformed infrared suggested a sequential oxidation mechanism,in which the main reaction steps of glycerol→glyceraldehyde→glyceric acid were very rapid to produce a high selectivity of formic acid even though the low glycerol conversion.This work presents an opportunity to study Ni NCs for the efficient electrocatalytic oxidation of biomass-derived polyhydroxyl platform molecules to produce value-added carboxylic acids.展开更多
The emergence of cesium lead halide perovskite materials stable at air opened new prospects for the optoelectronic industry.In this work we present an approach to fabricating a flexible green perovskite light-emitting...The emergence of cesium lead halide perovskite materials stable at air opened new prospects for the optoelectronic industry.In this work we present an approach to fabricating a flexible green perovskite light-emitting electrochemical cell(PeLEC)with a CsPbBr_(3)perovskite active layer using a highly-ordered silicon nanowire(Si NW)array as a distributed electrode integrated within a thin polydimethylsiloxane film(PDMS).Numerical simulations reveal that Si NWs-based distributed electrode aids the improvement of carrier injection into the perovskite layer with an increased thickness and,therefore,the enhancement of light-emitting performance.The X-ray diffraction study shows that the perovskite layer synthesized on the PDMS membrane with Si NWs has a similar crystal structure to the ones synthesized on planar Si wafers.We perform a comparative analysis of the light-emitting devices’properties fabricated on rigid silicon substrates and flexible Si NW-based membranes released from substrates.Due to possible potential barriers in a flexible PeLEC between the bottom electrode(made of a network of single-walled carbon nanotube film)and Si NWs,the electroluminescence performance and Ⅰ-V properties of flexible devices deteriorated compared to rigid devices.The developed PeLECs pave the way for further development of inorganic flexible uniformly light-emitting devices with improved properties.展开更多
Olfactory receptors(ORs),the largest vertebrate multigene family,exhibit wide copy number variation among taxa,ranging from~100 to 4000.The ecological importance of smell has been suggested to positively correlate wit...Olfactory receptors(ORs),the largest vertebrate multigene family,exhibit wide copy number variation among taxa,ranging from~100 to 4000.The ecological importance of smell has been suggested to positively correlate with OR gene number,though debate exists on whether the number of total ORs,functional ORs,or the percentage of pseudogenes matters most.While olfaction has been poorly studied in most birds,Turkey Vultures(Cathartes aura)demonstrate keen olfactory ability,capable of foraging using smell alone.In contrast,Black Vultures(Coragyps atratus)have been thought to primarily use vision to locate food.Comparison of the OR genes in these two New World vultures presents an opportunity to examine the dynamics of OR evolution in related avian species that may differ in olfactory abilities.Using a PCR and cloning approach with degenerate primers,we sampled the OR subgenome in Turkey and Black Vultures,as well as Red-tailed Hawks(Buteo jamaicensis)and the distantly related Chicken(Gallus gallus),neither of which are thought to use olfaction extensively.Our results indicate that Turkey Vultures have many more OR genes than Red-tailed Hawks or chickens.Surprisingly,Black Vultures had an intermediate number of OR genes.The number of OR genes we estimated in the Turkey Vulture was much greater than previously reported in studies that used short-read sequencing.Additionally,we found that OR genes from New World vultures and Red-tailed Hawks form clades that were distinct from the clade that included most chicken OR genes,indicating that chickens share few OR orthologs with New World vultures or hawks.As previously observed in other animal groups,pseudogenes appeared throughout all clades and their percentage varied among taxa.These findings suggest the OR gene family is highly dynamic,changing rapidly over evolutionary time,and that taxa may have distinct suites of ORs in their genomes.展开更多
Objectives:The phytochemical investigation of traditional herbal medicines holds significant promise for modern drug discovery,particularly in cancer therapy.This study aimed to evaluate the cytotoxicity,apoptosis ind...Objectives:The phytochemical investigation of traditional herbal medicines holds significant promise for modern drug discovery,particularly in cancer therapy.This study aimed to evaluate the cytotoxicity,apoptosis induction,and immune-modulatory activities of extracts from three herbal medicines with historical use in traditional medicine—Acanthopanax sessiliflorus,Phragmites communis,and Pinus densiflora,as well as their combined extract(GMAS 01/COM),on human lung cancer cells(A549)and normal cell lines,including murine macrophages(RAW 264.7)and human keratinocytes(HaCaT).Methods:Plant extracts were prepared using aqueous extraction,sonication,and rotary evaporation.The total phenolic and flavonoid contents were quantified using the Folin-Ciocalteu and AlCl3 colorimetric methods,respectively.Antioxidant potential was evaluated via 2,2-Diphenyl-1-picrylhydrazyl(DPPH)scavenging and reducing power assays.Cytotoxicity was assessed using an MTT assay,while reactive oxygen species(ROS)generation was quantified using a 2′,7′-Dichlorodihydrofluorescein diacetate(DCFH-DA)assay.Anticancer properties,including colony formation inhibition and migration suppression,were examined using colony formation and wound healing assays.The expression of apoptotic and inflammatory mediators was analysed through qPCR.Results:GMAS 01 selectively induced apoptosis in A549 cells without cytotoxic effects on RAW264.7 and HaCaT cells.Mechanistically,it elevated intracellular ROS and activated the intrinsic mitochondrial apoptotic pathway,evidenced by p53 upregulation,increased Bax,and decreased Bcl-2 expression.GMAS 01 also significantly inhibited colony formation and migration in A549 cells.In RAW264.7 cells,it reduced nitric oxide(NO)production and downregulated iNOS,COX-2,IL-6,and IL-8,indicating strong immunomodulatory activity.Conclusion:GMAS 01 exhibits potent antioxidant,anti-inflammatory,and anticancer effects,likely mediated through ROS-induced mitochondrial apoptosis.However,mechanistic interpretations are limited by the absence of protein-level validation and pathway inhibition studies.Upcoming studies should aim to verify the underlying mechanisms and evaluate their potential for real-world application.展开更多
Directed x-rays produced in the interaction of sub-picosecond laser pulses of moderate relativistic intensity with plasma of near-critical density are investigated. Synchrotron-like (betatron) radiation occurs in the ...Directed x-rays produced in the interaction of sub-picosecond laser pulses of moderate relativistic intensity with plasma of near-critical density are investigated. Synchrotron-like (betatron) radiation occurs in the process of direct laser acceleration (DLA) of electrons in a relativisticlaser channel when the electrons undergo transverse betatron oscillations in self-generated quasi-static electric and magnetic fields. In anexperiment at the PHELIX laser system, high-current directed beams of DLA electrons with a mean energy ten times higher than the ponderomotive potential and maximum energy up to 100 MeV were measured at 10^(19) W/cm^(2)laser intensity. The spectrum of directed x-raysin the range of 5–60 keV was evaluated using two sets of Ross filters placed at 0°and 10°to the laser pulse propagation axis. The differential x-ray absorption method allowed for absolute measurements of the angular-dependent photon fluence. We report 10^(13) photons/sr withenergies >5 keV measured at 0°to the laser axis and a brilliance of 10^(21) photons s^(−1) mm^(−2) mrad−2(0.1%BW)−1. The angular distributionof the emission has an FWHM of 14°–16°. Thanks to the ultra-high photon fluence, point-like radiation source, and ultra-short emissiontime, DLA-based keV backlighters are promising for various applications in high-energy-density research with kilojoule petawatt-class laserfacilities.展开更多
During injection treatments, bottomhole pressure measurements may significantly mismatch modeling results. We devise a computationally effective technique for interpretation of fluid injection in a wellbore interval w...During injection treatments, bottomhole pressure measurements may significantly mismatch modeling results. We devise a computationally effective technique for interpretation of fluid injection in a wellbore interval with multiple geological layers based on the bottomhole pressure measurements. The permeability, porosity and compressibility in each layer are initially setup, while the skin factor and partitioning of injected fluids among the zones during the injection are found as a solution of the problem. The problem takes into account Darcy flow and chemical interactions between the injected acids, diverter fluids and reservoir rock typical in modern matrix acidizing treatments. Using the synchronously recorded injection rate and bottomhole pressure, we evaluate skin factor changes in each layer and actual fluid placement into the reservoir during different pumping jobs: matrix acidizing, water control, sand control, scale squeezes and water flooding. The model is validated by comparison with a simulator used in industry. It gives opportunity to estimate efficiency of a matrix treatment job, role of every injection stage, and control fluid delivery to each layer in real time. The presented interpretation technique significantly improves accuracy of matrix treatments analysis by coupling the hydrodynamic model with records of pressure and injection rate during the treatment.展开更多
Biodegradable implants are critical for regenerative orthopaedic procedures,but they may suffer from too fast corrosion in human-body environment.This necessitates the synthesis of a suitable coating that may improve ...Biodegradable implants are critical for regenerative orthopaedic procedures,but they may suffer from too fast corrosion in human-body environment.This necessitates the synthesis of a suitable coating that may improve the corrosion resistance of these implants without compromising their mechanical integrity.In this study,an AZ91 magnesium alloy,as a representative for a biodegradable Mg implant material,was modified with a thin reduced graphene oxide(RGO)-calcium carbonate(CaCO_(3))composite coating.Detailed analytical and in-vitro electrochemical characterization reveals that this coating significantly improves the corrosion resistance and mechanical integrity,and thus has the potential to greatly extend the related application field.展开更多
The renewable energy industry is heavily reliant on rare earth elements,underscoring the need to develop resources and production.The objective of this work was to estimate coal ash resources and potential for extract...The renewable energy industry is heavily reliant on rare earth elements,underscoring the need to develop resources and production.The objective of this work was to estimate coal ash resources and potential for extraction of rare earth elements using data for the US.Data on spatiotemporal variability in coal ash resources and disposition were compiled from various federal databases and rare earth elements levels in ash were compiled from the literature.Results show that~52 gigatons(Gt)of coal were produced in the US(1950-2021).Power plants account for most of the coal use,particularly since 1980.Coal ash(5.3 Gt)represents a mean of 10%of coal by weight,ranging from 6%for subbituminous to 14%for lignite.About 70% of coal ash is potentially accessible for rare earth element extraction(1985-2021)and was disposed in landfills and ponds with the remaining coal ash used onsite or sold.Median values of total rare earth elements are much higher in ashes derived from the Appalachian Basin(median 431 mg/kg)than in the Illinois(282 mg/kg)or Powder River basins(264 mg/kg).Considering the market value of rare earth oxides,potentially accessible ash volumes,and percent rare earth element extraction(30%Appalachian and Illinois Basins;70% Powder River Basin)results in an estimated$8.4 billion value.This study provides fundamental information on accessible coal ash resources in the US,linkages to coal sources,and preliminary estimates of rare earth element levels for future development within the US.展开更多
A greenhouse experiment was conducted involving complete/intact 100 cm cores of a fragipan soil. The cores were maintained in moist conditions throughout the experiment as annual ryegrass (Lolium multiflorum) or festu...A greenhouse experiment was conducted involving complete/intact 100 cm cores of a fragipan soil. The cores were maintained in moist conditions throughout the experiment as annual ryegrass (Lolium multiflorum) or festulolium (Lolium spp and Fescue spp hybrid) were grown with and without additional surface applied amendments, including NaF, NaNO3, NaCl, and KCl. The results suggest a significant effect of annual ryegrass and festulolium on fragipan horizon degradation after 24 months. Annual ryegrass and festulolium were found to effectively change the structure of the fragipan horizon when planted on the soil surface and roots grew through the upper soil profile and penetrated into the fragipan. The fragipan structural change (degradation) appeared to increase with each planting sequence, particularly with soybean crop rotations. Sodium nitrate added to the soil surface with the growth of annual ryegrass resulted in a significant synergistic effect for degrading the fragipan horizon. The other amendments were not as effective in causing additional fragipan degradation.展开更多
A greenhouse experiment was conducted involving intact fragipan soil cores of 50 cm thickness after removing the topsoil horizons. The cores were maintained in moist condition throughout the experiment and received se...A greenhouse experiment was conducted involving intact fragipan soil cores of 50 cm thickness after removing the topsoil horizons. The cores were maintained in moist condition throughout the experiment and received several treatments with various amendments for different periods ranging from 9 to 17 months. The amendments included annual ryegrass or Festulolium residues, powder limestone and various humate compounds alone or in combination with the grass residues. The results suggested a significant effect of ryegrass and Festulolium in reducing penetration resistance into the top 10 cm of the fragipan within 9 - 17 months, particularly when used in combination with certain humate materials such as Leonardite. Apparently, this is the result of the release of certain soluble organic compounds from the plant residues or the humate amendments that increase the solubility of Si and Al associated with the fragipan brittleness, thus decreasing the density of the compacted fragipan material.展开更多
BACKGROUND Thus far,genetic analysis of patients clinically diagnosed with glycogen storage diseases(GSDs)in Thailand has not been reported.AIM To evaluate the clinical and biochemical profiles,molecular analysis and ...BACKGROUND Thus far,genetic analysis of patients clinically diagnosed with glycogen storage diseases(GSDs)in Thailand has not been reported.AIM To evaluate the clinical and biochemical profiles,molecular analysis and long-term outcomes of Thai children diagnosed with hepatic GSD.METHODS Children aged<18 years diagnosed with hepatic GSD and followed up at King Chulalongkorn Memorial Hospital were recruited.Whole-exome sequencing(WES)was performed to identify the causative gene variants.Medical records were assessed.RESULTS All eight children with histopathologically confirmed diagnosis were classified by WES into subtypes Ia(n=1),III(n=3),VI(n=3),and IX(n=1).A total number of 10 variants were identified including G6PC(n=1),AGL(n=4),PYGL(n=5),and PHKA2(n=1).AGL had two novel variants.The clinical manifestations were hepatomegaly(n=8),doll-like facies(n=3),wasting(n=2),and stunting(n=5).All patients showed hypoglycemia,transaminitis,and dyslipidemia.The mainstay of treatment was cornstarch supplementation and high-protein and low-lactosefructose diet.After a median follow-up time of 9.59 years,height turned to normal for age in 3/5 patients and none had malnutrition.Liver enzymes,blood sugar,and lipid profiles improved in all.CONCLUSION Hepatomegaly,transaminitis,and hypoglycemia are the hallmarks of GSD confirmed by liver histopathology.Molecular analysis can confirm the diagnosis or classify the subtype that might benefit from personalized treatment,prognosis,and long-term care.展开更多
基金funded by Korea Environmental Industry&Technology Institute(KEITI)through Wetland Ecosystem Value Evaluation and Carbon Absorption Value Promotion Technology Development Project of Korea Ministry of Environment(MOE)(RS-2022-KE002025).
文摘Urbanization and environmental degradation have led to significant declines in water quality and aquatic ecosystem health,highlighting the urgent need for effective restoration efforts.This study applies an integrated analysis approach to estimate the economic value and benefits of improvements in water quality and aquatic ecosystem services resulting from the Ecological Stream Restoration Project.Using survey data analyzed through the choice experiment(CE)method,we assessed respondents’preferences for various ecosystem services,including water-friendly services,ecological functions,water-level control,and water-quality purification.Three empirical analysis models—the Conditional Logit Model(CLM),Nested Logit Model(NL),and Error Component Logit Model(ECL)—were applied,with the ECL model identified as the most suitable for this study.From the physical impact assessment,we derived compensating variations to estimate the annual economic benefits of the project.The estimated annual economic value of water quality improvement due to the Anyangcheon Ecological Stream Restoration Project ranged from approximately KRW 10.54 billion to KRW 21.44 billion,while the economic value of aquatic ecosystem improvement was estimated to range from KRW 6.05 billion to KRW 12.30 billion annually.This study provides analytic framework that can inform future ecological restoration projects and sustainable water management policies.
基金funded by Korea Environmental Industry&Technology Institute(KEITI)through“Development of Aquatic Ecosystem Service Evaluation Indicators and Valuation Technology”of the Korea Ministry of Environment(MOE)(RS-2025-02214985).
文摘This study examines the empirical feasibility of quantitatively integrating environmental value information into Strategic Environmental Assessment(SEA).An analytical framework was established to incorporate environmental cost estimates into the SEA process by utilizing ecosystem service unit values provided by the Environmental Valuation Information System(EVIS),a national platform developed to support the evaluation of policies and projects.The framework was applied to a case study involving a multipurpose rural water development project in South Korea.Ecosystem service losses resulting from the project were quantified using biophysical indicators,such as vegetation biomass,forest area,and hydrological functions,and subsequently monetized through the application of the market price method,replacement cost method,and contingent valuation method.The total annual environmental cost was estimated to be approximately KRW 56.18 billion,with the majority attributable to losses in forest conservation and climate regulation services.These findings demonstrate that quantified environmental data can serve as a robust basis for alternative comparison and site evaluation within SEA.The study provides empirical evidence supporting the advancement of SEA from a predominantly procedural tool focused on environmental protection to a more comprehensive sustainability assessment framework that integrates environmental,economic,and social considerations.Furthermore,the results suggest that EVIS-based quantitative information holds potential for broader application in other national evaluation systems,such as preliminary feasibility studies and regulatory impact assessments.
文摘The electrocatalytic oxidation of glycerol toward formic acid is one of the most promising pathways for transformation and utilization of glycerol.Herein,a series of well-defined Ni_(n)(SR)_(2n) nanoclusters(n=4,5,6;denoted as Ni NCs)were prepared for the electrocatalytic glycerol oxidation toward formic acid,in which Ni_(6)-PET-50CV afforded the most excellent electrocatalytic performance with a high formic acid selectivity of 93% and a high glycerol conversion of 86%.This was attributed to the lowest charge transfer impedance and the most rapid reaction kinetics.Combined electrochemical measurements and X-ray absorption fine structure measurements revealed that the structures of Ni NCs remained intact after CV scanning pretreatment and electrocatalysis via forming the Ni–O bond.Additionally,the kinetic studies and in-situ Fourier transformed infrared suggested a sequential oxidation mechanism,in which the main reaction steps of glycerol→glyceraldehyde→glyceric acid were very rapid to produce a high selectivity of formic acid even though the low glycerol conversion.This work presents an opportunity to study Ni NCs for the efficient electrocatalytic oxidation of biomass-derived polyhydroxyl platform molecules to produce value-added carboxylic acids.
基金the Russian Science Foundation Project No.22-79-10286,https://rscf.ru/project/22-7910286/(synthesis of perovskites, PeLEC fabrication)the Russian Science Foundation Project No.23-79-01151 for NW/PDMS membrane fabrication and PeLEC characterization+1 种基金the Ministry of Science and Higher Education of the Russian Federation (Project. FZSR-2020-0007 within the framework of state task no. 075-03-2020-097/1) for the support of SWCNT synthesisthe Ministry of Science and Higher Education of the Russian Federation (Project FSRM-2022-0007) for NW fabrication
文摘The emergence of cesium lead halide perovskite materials stable at air opened new prospects for the optoelectronic industry.In this work we present an approach to fabricating a flexible green perovskite light-emitting electrochemical cell(PeLEC)with a CsPbBr_(3)perovskite active layer using a highly-ordered silicon nanowire(Si NW)array as a distributed electrode integrated within a thin polydimethylsiloxane film(PDMS).Numerical simulations reveal that Si NWs-based distributed electrode aids the improvement of carrier injection into the perovskite layer with an increased thickness and,therefore,the enhancement of light-emitting performance.The X-ray diffraction study shows that the perovskite layer synthesized on the PDMS membrane with Si NWs has a similar crystal structure to the ones synthesized on planar Si wafers.We perform a comparative analysis of the light-emitting devices’properties fabricated on rigid silicon substrates and flexible Si NW-based membranes released from substrates.Due to possible potential barriers in a flexible PeLEC between the bottom electrode(made of a network of single-walled carbon nanotube film)and Si NWs,the electroluminescence performance and Ⅰ-V properties of flexible devices deteriorated compared to rigid devices.The developed PeLECs pave the way for further development of inorganic flexible uniformly light-emitting devices with improved properties.
基金supported by a grant from the Singer Biology Fund at the University of Florida。
文摘Olfactory receptors(ORs),the largest vertebrate multigene family,exhibit wide copy number variation among taxa,ranging from~100 to 4000.The ecological importance of smell has been suggested to positively correlate with OR gene number,though debate exists on whether the number of total ORs,functional ORs,or the percentage of pseudogenes matters most.While olfaction has been poorly studied in most birds,Turkey Vultures(Cathartes aura)demonstrate keen olfactory ability,capable of foraging using smell alone.In contrast,Black Vultures(Coragyps atratus)have been thought to primarily use vision to locate food.Comparison of the OR genes in these two New World vultures presents an opportunity to examine the dynamics of OR evolution in related avian species that may differ in olfactory abilities.Using a PCR and cloning approach with degenerate primers,we sampled the OR subgenome in Turkey and Black Vultures,as well as Red-tailed Hawks(Buteo jamaicensis)and the distantly related Chicken(Gallus gallus),neither of which are thought to use olfaction extensively.Our results indicate that Turkey Vultures have many more OR genes than Red-tailed Hawks or chickens.Surprisingly,Black Vultures had an intermediate number of OR genes.The number of OR genes we estimated in the Turkey Vulture was much greater than previously reported in studies that used short-read sequencing.Additionally,we found that OR genes from New World vultures and Red-tailed Hawks form clades that were distinct from the clade that included most chicken OR genes,indicating that chickens share few OR orthologs with New World vultures or hawks.As previously observed in other animal groups,pseudogenes appeared throughout all clades and their percentage varied among taxa.These findings suggest the OR gene family is highly dynamic,changing rapidly over evolutionary time,and that taxa may have distinct suites of ORs in their genomes.
文摘Objectives:The phytochemical investigation of traditional herbal medicines holds significant promise for modern drug discovery,particularly in cancer therapy.This study aimed to evaluate the cytotoxicity,apoptosis induction,and immune-modulatory activities of extracts from three herbal medicines with historical use in traditional medicine—Acanthopanax sessiliflorus,Phragmites communis,and Pinus densiflora,as well as their combined extract(GMAS 01/COM),on human lung cancer cells(A549)and normal cell lines,including murine macrophages(RAW 264.7)and human keratinocytes(HaCaT).Methods:Plant extracts were prepared using aqueous extraction,sonication,and rotary evaporation.The total phenolic and flavonoid contents were quantified using the Folin-Ciocalteu and AlCl3 colorimetric methods,respectively.Antioxidant potential was evaluated via 2,2-Diphenyl-1-picrylhydrazyl(DPPH)scavenging and reducing power assays.Cytotoxicity was assessed using an MTT assay,while reactive oxygen species(ROS)generation was quantified using a 2′,7′-Dichlorodihydrofluorescein diacetate(DCFH-DA)assay.Anticancer properties,including colony formation inhibition and migration suppression,were examined using colony formation and wound healing assays.The expression of apoptotic and inflammatory mediators was analysed through qPCR.Results:GMAS 01 selectively induced apoptosis in A549 cells without cytotoxic effects on RAW264.7 and HaCaT cells.Mechanistically,it elevated intracellular ROS and activated the intrinsic mitochondrial apoptotic pathway,evidenced by p53 upregulation,increased Bax,and decreased Bcl-2 expression.GMAS 01 also significantly inhibited colony formation and migration in A549 cells.In RAW264.7 cells,it reduced nitric oxide(NO)production and downregulated iNOS,COX-2,IL-6,and IL-8,indicating strong immunomodulatory activity.Conclusion:GMAS 01 exhibits potent antioxidant,anti-inflammatory,and anticancer effects,likely mediated through ROS-induced mitochondrial apoptosis.However,mechanistic interpretations are limited by the absence of protein-level validation and pathway inhibition studies.Upcoming studies should aim to verify the underlying mechanisms and evaluate their potential for real-world application.
基金supported by the Czech Ministry of Education,Youth and Sports(Project No.CZ.02.2.69/0.0/0.0/18_053/0016980)the Grant Agency of the Czech Republic(Grant No.GM23-05027M).
文摘Directed x-rays produced in the interaction of sub-picosecond laser pulses of moderate relativistic intensity with plasma of near-critical density are investigated. Synchrotron-like (betatron) radiation occurs in the process of direct laser acceleration (DLA) of electrons in a relativisticlaser channel when the electrons undergo transverse betatron oscillations in self-generated quasi-static electric and magnetic fields. In anexperiment at the PHELIX laser system, high-current directed beams of DLA electrons with a mean energy ten times higher than the ponderomotive potential and maximum energy up to 100 MeV were measured at 10^(19) W/cm^(2)laser intensity. The spectrum of directed x-raysin the range of 5–60 keV was evaluated using two sets of Ross filters placed at 0°and 10°to the laser pulse propagation axis. The differential x-ray absorption method allowed for absolute measurements of the angular-dependent photon fluence. We report 10^(13) photons/sr withenergies >5 keV measured at 0°to the laser axis and a brilliance of 10^(21) photons s^(−1) mm^(−2) mrad−2(0.1%BW)−1. The angular distributionof the emission has an FWHM of 14°–16°. Thanks to the ultra-high photon fluence, point-like radiation source, and ultra-short emissiontime, DLA-based keV backlighters are promising for various applications in high-energy-density research with kilojoule petawatt-class laserfacilities.
文摘During injection treatments, bottomhole pressure measurements may significantly mismatch modeling results. We devise a computationally effective technique for interpretation of fluid injection in a wellbore interval with multiple geological layers based on the bottomhole pressure measurements. The permeability, porosity and compressibility in each layer are initially setup, while the skin factor and partitioning of injected fluids among the zones during the injection are found as a solution of the problem. The problem takes into account Darcy flow and chemical interactions between the injected acids, diverter fluids and reservoir rock typical in modern matrix acidizing treatments. Using the synchronously recorded injection rate and bottomhole pressure, we evaluate skin factor changes in each layer and actual fluid placement into the reservoir during different pumping jobs: matrix acidizing, water control, sand control, scale squeezes and water flooding. The model is validated by comparison with a simulator used in industry. It gives opportunity to estimate efficiency of a matrix treatment job, role of every injection stage, and control fluid delivery to each layer in real time. The presented interpretation technique significantly improves accuracy of matrix treatments analysis by coupling the hydrodynamic model with records of pressure and injection rate during the treatment.
文摘Biodegradable implants are critical for regenerative orthopaedic procedures,but they may suffer from too fast corrosion in human-body environment.This necessitates the synthesis of a suitable coating that may improve the corrosion resistance of these implants without compromising their mechanical integrity.In this study,an AZ91 magnesium alloy,as a representative for a biodegradable Mg implant material,was modified with a thin reduced graphene oxide(RGO)-calcium carbonate(CaCO_(3))composite coating.Detailed analytical and in-vitro electrochemical characterization reveals that this coating significantly improves the corrosion resistance and mechanical integrity,and thus has the potential to greatly extend the related application field.
基金This work was funded by the US DOE Ofice of Fossil Energy and Carbon Management,under the project titled“Carbon Ore,Rare Earth and Critical Minerals(CORE-CM)Initiative for U.S.Basins”(DE-FOA-0002364,2022)。
文摘The renewable energy industry is heavily reliant on rare earth elements,underscoring the need to develop resources and production.The objective of this work was to estimate coal ash resources and potential for extraction of rare earth elements using data for the US.Data on spatiotemporal variability in coal ash resources and disposition were compiled from various federal databases and rare earth elements levels in ash were compiled from the literature.Results show that~52 gigatons(Gt)of coal were produced in the US(1950-2021).Power plants account for most of the coal use,particularly since 1980.Coal ash(5.3 Gt)represents a mean of 10%of coal by weight,ranging from 6%for subbituminous to 14%for lignite.About 70% of coal ash is potentially accessible for rare earth element extraction(1985-2021)and was disposed in landfills and ponds with the remaining coal ash used onsite or sold.Median values of total rare earth elements are much higher in ashes derived from the Appalachian Basin(median 431 mg/kg)than in the Illinois(282 mg/kg)or Powder River basins(264 mg/kg).Considering the market value of rare earth oxides,potentially accessible ash volumes,and percent rare earth element extraction(30%Appalachian and Illinois Basins;70% Powder River Basin)results in an estimated$8.4 billion value.This study provides fundamental information on accessible coal ash resources in the US,linkages to coal sources,and preliminary estimates of rare earth element levels for future development within the US.
文摘A greenhouse experiment was conducted involving complete/intact 100 cm cores of a fragipan soil. The cores were maintained in moist conditions throughout the experiment as annual ryegrass (Lolium multiflorum) or festulolium (Lolium spp and Fescue spp hybrid) were grown with and without additional surface applied amendments, including NaF, NaNO3, NaCl, and KCl. The results suggest a significant effect of annual ryegrass and festulolium on fragipan horizon degradation after 24 months. Annual ryegrass and festulolium were found to effectively change the structure of the fragipan horizon when planted on the soil surface and roots grew through the upper soil profile and penetrated into the fragipan. The fragipan structural change (degradation) appeared to increase with each planting sequence, particularly with soybean crop rotations. Sodium nitrate added to the soil surface with the growth of annual ryegrass resulted in a significant synergistic effect for degrading the fragipan horizon. The other amendments were not as effective in causing additional fragipan degradation.
文摘A greenhouse experiment was conducted involving intact fragipan soil cores of 50 cm thickness after removing the topsoil horizons. The cores were maintained in moist condition throughout the experiment and received several treatments with various amendments for different periods ranging from 9 to 17 months. The amendments included annual ryegrass or Festulolium residues, powder limestone and various humate compounds alone or in combination with the grass residues. The results suggested a significant effect of ryegrass and Festulolium in reducing penetration resistance into the top 10 cm of the fragipan within 9 - 17 months, particularly when used in combination with certain humate materials such as Leonardite. Apparently, this is the result of the release of certain soluble organic compounds from the plant residues or the humate amendments that increase the solubility of Si and Al associated with the fragipan brittleness, thus decreasing the density of the compacted fragipan material.
基金Supported by Ratchadaphiseksomphot Fund,Graduate Affairs,Faculty of Medicine,Chulalongkorn University,No.GA66/020Ratchadaphiseksomphot Fund,Chulalongkorn University,No.RCU_H_64_007_30.
文摘BACKGROUND Thus far,genetic analysis of patients clinically diagnosed with glycogen storage diseases(GSDs)in Thailand has not been reported.AIM To evaluate the clinical and biochemical profiles,molecular analysis and long-term outcomes of Thai children diagnosed with hepatic GSD.METHODS Children aged<18 years diagnosed with hepatic GSD and followed up at King Chulalongkorn Memorial Hospital were recruited.Whole-exome sequencing(WES)was performed to identify the causative gene variants.Medical records were assessed.RESULTS All eight children with histopathologically confirmed diagnosis were classified by WES into subtypes Ia(n=1),III(n=3),VI(n=3),and IX(n=1).A total number of 10 variants were identified including G6PC(n=1),AGL(n=4),PYGL(n=5),and PHKA2(n=1).AGL had two novel variants.The clinical manifestations were hepatomegaly(n=8),doll-like facies(n=3),wasting(n=2),and stunting(n=5).All patients showed hypoglycemia,transaminitis,and dyslipidemia.The mainstay of treatment was cornstarch supplementation and high-protein and low-lactosefructose diet.After a median follow-up time of 9.59 years,height turned to normal for age in 3/5 patients and none had malnutrition.Liver enzymes,blood sugar,and lipid profiles improved in all.CONCLUSION Hepatomegaly,transaminitis,and hypoglycemia are the hallmarks of GSD confirmed by liver histopathology.Molecular analysis can confirm the diagnosis or classify the subtype that might benefit from personalized treatment,prognosis,and long-term care.
