The development of sustainable,eco-friendly polyesters from renewable resources is crucial for reducing dependence on petroleum-based plastics.However,despite advances in microbial pro-duction of bioplastics,significa...The development of sustainable,eco-friendly polyesters from renewable resources is crucial for reducing dependence on petroleum-based plastics.However,despite advances in microbial pro-duction of bioplastics,significant challenges remain in achieving high conversion efficiency and scalability for industrial applications.This study is the first to report the synthesis of a 100%bio-based polyester using both 1,12-dodecanedioic acid(1,12-diacid)and 1,12-dodecanediol(1,12-diol)via a two-step microbial bioconversion from a single plant oil-derived alkane.An engineered Candida tropicalis strain produced 150 g/L of 1,12-diacid with a productivity of 1.53 g/(L·h)in a 5 L fed-batch system using a two-phase biotransformation strategy.Escherichia coli engineered to express carboxylic acid reductase,which reduces carboxylic acids to aldehydes,and its ac-tivation enzyme phosphopantetheinyl transferase,converted 1,12-diacid into 68 g/L 1,12-diol with a productivity of 1.42 g/(L·h)in a 5 L fed-batch system,representing high titer and pro-ductivity for microbial production of long-chainα,ω-diols.Both monomer production processes were successfully scaled up to a 50 L pilot fermenter,validating their potential for industrial implementation.A highly efficient downstream purification process was developed,achieving>98%purity and recovery rates for both monomers.The bio-derived monomers enabled the syn-thesis of polyesters with molecular weight and thermal characteristics similar to petroleum-based monomers of the same chemical structure.This integrated approach establishes a robust and scal-able microbial platform that converts renewable lipid feedstocks into fully bio-based polyesters,thereby demonstrating an environmentally sustainable and industrially viable route to circular bioeconomy-based polyester production.展开更多
基金supported by the KEIT R&D Program(No.20025698&00432188)funded by the Ministry of Trade,Industry&Energy(Republic of Korea).
文摘The development of sustainable,eco-friendly polyesters from renewable resources is crucial for reducing dependence on petroleum-based plastics.However,despite advances in microbial pro-duction of bioplastics,significant challenges remain in achieving high conversion efficiency and scalability for industrial applications.This study is the first to report the synthesis of a 100%bio-based polyester using both 1,12-dodecanedioic acid(1,12-diacid)and 1,12-dodecanediol(1,12-diol)via a two-step microbial bioconversion from a single plant oil-derived alkane.An engineered Candida tropicalis strain produced 150 g/L of 1,12-diacid with a productivity of 1.53 g/(L·h)in a 5 L fed-batch system using a two-phase biotransformation strategy.Escherichia coli engineered to express carboxylic acid reductase,which reduces carboxylic acids to aldehydes,and its ac-tivation enzyme phosphopantetheinyl transferase,converted 1,12-diacid into 68 g/L 1,12-diol with a productivity of 1.42 g/(L·h)in a 5 L fed-batch system,representing high titer and pro-ductivity for microbial production of long-chainα,ω-diols.Both monomer production processes were successfully scaled up to a 50 L pilot fermenter,validating their potential for industrial implementation.A highly efficient downstream purification process was developed,achieving>98%purity and recovery rates for both monomers.The bio-derived monomers enabled the syn-thesis of polyesters with molecular weight and thermal characteristics similar to petroleum-based monomers of the same chemical structure.This integrated approach establishes a robust and scal-able microbial platform that converts renewable lipid feedstocks into fully bio-based polyesters,thereby demonstrating an environmentally sustainable and industrially viable route to circular bioeconomy-based polyester production.
