Numerous researchers and institutions have been developing in ovo sexing technologies to improve animal welfare by identifying male embryos in an early embryonic stage and disposing of them before pain perception.This...Numerous researchers and institutions have been developing in ovo sexing technologies to improve animal welfare by identifying male embryos in an early embryonic stage and disposing of them before pain perception.This review gives a complete overview of the technological approaches reported in papers and patents by performing a thorough search using Web of Science and Patstat/Espacenet databases for papers and patents,respectively.Based on a total of 49 papers and 115 patent families reported until May 2023 worldwide,11 technology categories were defined:6 non-optical and 5 optical techniques.Every category was described for its characteristics while assessing its potential for application.Next,the dynamics of the publications of in ovo sexing techniques in both paper and patent fields were described through growth curves,and the interest or actual status was visualized using the number of paper citations and the actual legal status of the patents.When comparing the reported technologies in papers to those in patents,scientific gaps were observed,as some of the patented technologies were not reported in the scientific literature,e.g.,ion mobility and mass spectrometry approaches.Generally,more diverse approaches in all categories were found in patents,although they do require more scientific evidence through papers or industrial adoption to prove their robustness.Moreover,although there is a recent trend for non-invasive techniques,invasive methods like analyzing DNA through PCR or hormones through immunosensing are still being reported(and might continue to be)in papers and patents.It was also observed that none of the technologies complies with all the industry requirements,although 5 companies already entered the market.On the one hand,more research and harmony between consumers,industry,and governments is necessary.On the other hand,close monitoring of the market performance of the currently available techniques will offer valuable insights into the potential and expectations of in ovo sexing techniques in the poultry industry.展开更多
Optical chemical sensor based on immobilesed pararosaniline into sol-gel matrix tetraethyl orthosilicate (TEOS) is a simple tool that can be used to detect the presence of formalin (formaldehide) in food. Pararosaline...Optical chemical sensor based on immobilesed pararosaniline into sol-gel matrix tetraethyl orthosilicate (TEOS) is a simple tool that can be used to detect the presence of formalin (formaldehide) in food. Pararosaline in sol-gel matrix was developed when contacted with food sample that contains formalin. The optical signal was produced by changing color from purple to yellow, that can be used to detect quantitative formaldehide in sample. The results, chemo sensor optic, have characteristic, maximum wave length 576.42 nm, with linier range 0-100 ppm, linearity coefficient R2 = 0.999, limit detection (LOD) 0.504 ppm, limit of quantification (LOQ) 1.680 ppm, sensitivity 0.087, disturbed matrix selectivity 1.716 %. The optimum is operational at pH 4, and response time at 150 seconds of 2 ppm. This sensor can be used to detect formalin in food sample in a simple mode and reusable for 4 times application. In addition, the sensor can be regenerated using展开更多
The stable chromogenic radical 1,1'-diphenyl-2-picrylhydrazyl(DPPH) solution was immobilized on the microwell plate as dry reagent to construct a simple antioxidant sensor. Then, a regular flatbed scanner was used...The stable chromogenic radical 1,1'-diphenyl-2-picrylhydrazyl(DPPH) solution was immobilized on the microwell plate as dry reagent to construct a simple antioxidant sensor. Then, a regular flatbed scanner was used as microplate reader to obtain analytical parameters for antioxidant assay using one-shot optical sensors as scanometry technique. Variables affecting the acquisition of the images were optimized and the analytical parameters are obtained from an area of the sensing zone inside microwell using the average luminosity of the sensing zone captured as the mean of red, green, and blue(RGB)value using Image J~program. By using this RGB value as sensor response, it is possible to determine antioxidant capacity in the range 1–25 ppm as gallic acid equivalent(GAE) with the response time of 9 min. The reproducibility of sensor was good(RSD<1%) with recovery at 93%–96%. The antioxidant sensor was applied to the plant extracts, such as sappan wood and Turmeric Rhizome. The results are good when compared to the same procedure using a UV/Vis spectrophotometer.展开更多
A novel triple lines lateral-flow assay （LFA） with enhanced sensitivity for the detection of Leishmania infantum DNA in dog blood samples was designed and successfully applied. The enhanced LFA methodology takes adv...A novel triple lines lateral-flow assay （LFA） with enhanced sensitivity for the detection of Leishmania infantum DNA in dog blood samples was designed and successfully applied. The enhanced LFA methodology takes advantage of the gold nanoparticle tags （AuNPs） conjugated to polyclonal secondary antibodies, which recognize anti-FITC antibodies. The polyclonal nature of the secondary antibodies allows for multiple binding to primary antibodies, leading to enhanced AuNP plasmonics signal. Furthermore, endogenous control consisting of the amplified dog 18S rRNA gene was introduced to avoid false negatives. Using this strategy, 0.038 spiked Leishmania parasites per DNA amplification reaction （1 parasite/100 μL of DNA sample） were detected. Detection limit of LFA was found to be lower than that of the conventional techniques. In summary, our novel LFA design is a universal and simple sensing altemative that can be extended to several other biosensing scenarios.展开更多
A novel, cheap, disposable and single-use nanoparticles-based nanochannel platform assembled on a flexible substrate for label-free immunosensing is pre- sented. This sensing platform is formed by the dip-coating of a...A novel, cheap, disposable and single-use nanoparticles-based nanochannel platform assembled on a flexible substrate for label-free immunosensing is pre- sented. This sensing platform is formed by the dip-coating of a homogeneous and assembled monolayer of carboxylated polystyrene nanospheres （PS, 200 and 500 nm-sized） onto the working area of flexible screen-printed indium tin oxide/polyethylene terephthalate （ITO/PET） electrodes. The spaces between the self-assembled nanospheres generate well-ordered nanochannels, with inter-PS particles distances of around 65 and 24 nm respectively. The formed nanochannels are used for the effective immobilization of antibodies and subsequent protein detection based on the monitoring of [Fe（CN）6]^4- flow through diffusion and the decrease in the differential pulse voltammetric signal upon immunocomplex formation. The obtained sensing system is nanochannel-size dependent and allows human immunoglobulin G （IgG） （chosen as a model analyte） to be detected at levels of 580 ng/mL. The system also exhibits an excellent specificity against other proteins present in real samples and shows good performance with a human urine sample. The developed device represents an integrated and simple biodetection system which overcomes many of the limitations of previously reported nanochannels-based approaches and can be extended in the future to several other immuno and DNA detection systems.展开更多
An analytical and experimental study of nanofilm aluminum (Al) for ultra-high dynamic range surface plasmon resonance (SPR) biosensor is presented in this article. A thin film of 16 nm Al is proposed for metallic sens...An analytical and experimental study of nanofilm aluminum (Al) for ultra-high dynamic range surface plasmon resonance (SPR) biosensor is presented in this article. A thin film of 16 nm Al is proposed for metallic sensing layer for SPR .s*ensoi For the protective layer, a 10 nm of gold (Au) layer was configured on top of Al as a protection layer. This ultra-high dynamic range of SPR biosensor reached the bulk refractive index sample limit up to 1.45 RIU. For the analytical study, with the assumption of anisotropic refractive indices experiment, the dynamic range showed a refractive index value of around 1.58 RIU. The refractive index value limit achieved by the proposed sensing design is potentially implemented in various applications, such as in chemical detection and environ- mental monitoring study with high refractive index solution sample. The experimental results are presented as a proof-of-concept of the proposed idea.展开更多
基金the Foundation for Food and Agricultural Research[EggTech-0000000028]the Research Foundation-Flanders[SB project 1SC7219N and SB project 1S54823N].
文摘Numerous researchers and institutions have been developing in ovo sexing technologies to improve animal welfare by identifying male embryos in an early embryonic stage and disposing of them before pain perception.This review gives a complete overview of the technological approaches reported in papers and patents by performing a thorough search using Web of Science and Patstat/Espacenet databases for papers and patents,respectively.Based on a total of 49 papers and 115 patent families reported until May 2023 worldwide,11 technology categories were defined:6 non-optical and 5 optical techniques.Every category was described for its characteristics while assessing its potential for application.Next,the dynamics of the publications of in ovo sexing techniques in both paper and patent fields were described through growth curves,and the interest or actual status was visualized using the number of paper citations and the actual legal status of the patents.When comparing the reported technologies in papers to those in patents,scientific gaps were observed,as some of the patented technologies were not reported in the scientific literature,e.g.,ion mobility and mass spectrometry approaches.Generally,more diverse approaches in all categories were found in patents,although they do require more scientific evidence through papers or industrial adoption to prove their robustness.Moreover,although there is a recent trend for non-invasive techniques,invasive methods like analyzing DNA through PCR or hormones through immunosensing are still being reported(and might continue to be)in papers and patents.It was also observed that none of the technologies complies with all the industry requirements,although 5 companies already entered the market.On the one hand,more research and harmony between consumers,industry,and governments is necessary.On the other hand,close monitoring of the market performance of the currently available techniques will offer valuable insights into the potential and expectations of in ovo sexing techniques in the poultry industry.
文摘Optical chemical sensor based on immobilesed pararosaniline into sol-gel matrix tetraethyl orthosilicate (TEOS) is a simple tool that can be used to detect the presence of formalin (formaldehide) in food. Pararosaline in sol-gel matrix was developed when contacted with food sample that contains formalin. The optical signal was produced by changing color from purple to yellow, that can be used to detect quantitative formaldehide in sample. The results, chemo sensor optic, have characteristic, maximum wave length 576.42 nm, with linier range 0-100 ppm, linearity coefficient R2 = 0.999, limit detection (LOD) 0.504 ppm, limit of quantification (LOQ) 1.680 ppm, sensitivity 0.087, disturbed matrix selectivity 1.716 %. The optimum is operational at pH 4, and response time at 150 seconds of 2 ppm. This sensor can be used to detect formalin in food sample in a simple mode and reusable for 4 times application. In addition, the sensor can be regenerated using
文摘The stable chromogenic radical 1,1'-diphenyl-2-picrylhydrazyl(DPPH) solution was immobilized on the microwell plate as dry reagent to construct a simple antioxidant sensor. Then, a regular flatbed scanner was used as microplate reader to obtain analytical parameters for antioxidant assay using one-shot optical sensors as scanometry technique. Variables affecting the acquisition of the images were optimized and the analytical parameters are obtained from an area of the sensing zone inside microwell using the average luminosity of the sensing zone captured as the mean of red, green, and blue(RGB)value using Image J~program. By using this RGB value as sensor response, it is possible to determine antioxidant capacity in the range 1–25 ppm as gallic acid equivalent(GAE) with the response time of 9 min. The reproducibility of sensor was good(RSD<1%) with recovery at 93%–96%. The antioxidant sensor was applied to the plant extracts, such as sappan wood and Turmeric Rhizome. The results are good when compared to the same procedure using a UV/Vis spectrophotometer.
文摘A novel triple lines lateral-flow assay （LFA） with enhanced sensitivity for the detection of Leishmania infantum DNA in dog blood samples was designed and successfully applied. The enhanced LFA methodology takes advantage of the gold nanoparticle tags （AuNPs） conjugated to polyclonal secondary antibodies, which recognize anti-FITC antibodies. The polyclonal nature of the secondary antibodies allows for multiple binding to primary antibodies, leading to enhanced AuNP plasmonics signal. Furthermore, endogenous control consisting of the amplified dog 18S rRNA gene was introduced to avoid false negatives. Using this strategy, 0.038 spiked Leishmania parasites per DNA amplification reaction （1 parasite/100 μL of DNA sample） were detected. Detection limit of LFA was found to be lower than that of the conventional techniques. In summary, our novel LFA design is a universal and simple sensing altemative that can be extended to several other biosensing scenarios.
文摘A novel, cheap, disposable and single-use nanoparticles-based nanochannel platform assembled on a flexible substrate for label-free immunosensing is pre- sented. This sensing platform is formed by the dip-coating of a homogeneous and assembled monolayer of carboxylated polystyrene nanospheres （PS, 200 and 500 nm-sized） onto the working area of flexible screen-printed indium tin oxide/polyethylene terephthalate （ITO/PET） electrodes. The spaces between the self-assembled nanospheres generate well-ordered nanochannels, with inter-PS particles distances of around 65 and 24 nm respectively. The formed nanochannels are used for the effective immobilization of antibodies and subsequent protein detection based on the monitoring of [Fe（CN）6]^4- flow through diffusion and the decrease in the differential pulse voltammetric signal upon immunocomplex formation. The obtained sensing system is nanochannel-size dependent and allows human immunoglobulin G （IgG） （chosen as a model analyte） to be detected at levels of 580 ng/mL. The system also exhibits an excellent specificity against other proteins present in real samples and shows good performance with a human urine sample. The developed device represents an integrated and simple biodetection system which overcomes many of the limitations of previously reported nanochannels-based approaches and can be extended in the future to several other immuno and DNA detection systems.
基金This research was funded in part by Ministry of Science and Technology(MOST)(Taiwan,China)under the contract number ofMOST 107-2218-E-182-008 and MOST 106-2221-E-182-041,also Chang Gung Memorial Hospital Grant with the contract number of CMRPDG0151.The authors thank the Indonesian Institute of Sciences and Chang Gung University for supporting this research.B.A.P.wholeheartedly thanks Chang Gung University,Taiwan,China for the visiting scholar invitation under the grant number BMRP741.
文摘An analytical and experimental study of nanofilm aluminum (Al) for ultra-high dynamic range surface plasmon resonance (SPR) biosensor is presented in this article. A thin film of 16 nm Al is proposed for metallic sensing layer for SPR .s*ensoi For the protective layer, a 10 nm of gold (Au) layer was configured on top of Al as a protection layer. This ultra-high dynamic range of SPR biosensor reached the bulk refractive index sample limit up to 1.45 RIU. For the analytical study, with the assumption of anisotropic refractive indices experiment, the dynamic range showed a refractive index value of around 1.58 RIU. The refractive index value limit achieved by the proposed sensing design is potentially implemented in various applications, such as in chemical detection and environ- mental monitoring study with high refractive index solution sample. The experimental results are presented as a proof-of-concept of the proposed idea.
