Backgrounds: While there’s developing proof aimed toward improving embryo implantation thru a focal point on great development, restrained studies have been performed on enhancing endometrial receptivity. Intrauterin...Backgrounds: While there’s developing proof aimed toward improving embryo implantation thru a focal point on great development, restrained studies have been performed on enhancing endometrial receptivity. Intrauterine Laser Therapy (LT) can be powerful in selling endometrial cell proliferation, therefore enhancing the achievement of assisted reproductive techniques (ART). The contemporary look at aimed to research the effectiveness of effective intrauterine lasers in growing endometrial thickness and the achievement of being pregnant rate. Materials and Methods: In the current clinical randomized trial (RTC) study, the infertile women (20 - 42 years old) referred to the infertility clinic in 2023-2024 who were candidates for IVF treatment with recurrent implantation failure (RIF) history were included. The patients were divided into two main groups: the intervention group;low level laser therapy (LLLT) after hormone administration) (n = 52) and the control group (hormone administration without LT) (n = 52). The IVF success rate and change in endometrial thickness before and after the LT were compared in groups. Results: There was a significant difference between groups (p Conclusion: It appears that the incorporation of Intrauterine LT in the realm of infertility could significantly impact as a novel supplementary treatment in improving endometrial receptivity and pregnancy rate.展开更多
Mesenchymal stem cell(MSC)therapy is entering a challenging phase after completion of many preclinical and clinical trials.Among the major hurdles encountered in MSC therapy are inconsistent stem cell potency,poor cel...Mesenchymal stem cell(MSC)therapy is entering a challenging phase after completion of many preclinical and clinical trials.Among the major hurdles encountered in MSC therapy are inconsistent stem cell potency,poor cell engraftment and survival,and age/disease-related host tissue impairment.The recognition that MSCs primarily mediate therapeutic benefits through paracrine mechanisms independent of cell differentiation provides a promising framework for enhancing stem cell potency and therapeutic benefits.Several MSC priming approaches are highlighted,which will likely allow us to harness the full potential of adult stem cells for their future routine clinical use.展开更多
The mechanical properties, chemical properties and biocompatibility of Mg-3Sn-0.5Mn alloy were tested. A series of in vitro evaluations such as tensile test, static and dynamic immersion test, hemocompatibility test a...The mechanical properties, chemical properties and biocompatibility of Mg-3Sn-0.5Mn alloy were tested. A series of in vitro evaluations such as tensile test, static and dynamic immersion test, hemocompatibility test as well as cytotoxicity test were presented, with commercial magnesium alloy WE43 as the control. Mg-3Sn-0.5Mn alloy possesses suitable strength and superior ductility compared with WE43 and AZ31. Static immersion and dynamic degradation tests showed more uniform degradation with a more moderate rate for Mg-3Sn-0.5Mn alloy （0.34 mm/y in static condition and 0.25 mm/y in dynamic condition） compared with WE43 alloy （0.42 mm/y in static condition and 0.33 mm/y in dynamic condition） in Hank＇s solution. Blood compatibility evaluation suggested that Mg-3Sn-0.5Mn alloy had no destructive effect on erythrocyte and showed excellent anti-thrombogenicity to blood system. Besides, Mg-3Sn-0.5Mn alloy showed no inhibition effect to L929 metabolic activity and mild toxicity to vascular smooth muscle cell （VSMC） in preliminary cell viability assessment. By considering its excellent mechanical strength, corrosion resistance, low ion release rate and good biocompatibility, Mg-3Sn-0.5Mn alloy may be a promising economical candidate as biomedical implant material for load-bearing clinical applications in the future.展开更多
Background Cilostazol is a type 3 phosphodiesterase inhibitor which has been previously demonstrated to prevent the occurrence of tachyarrhythmia and improve defibrillation efficacy. However, the mechanism for this be...Background Cilostazol is a type 3 phosphodiesterase inhibitor which has been previously demonstrated to prevent the occurrence of tachyarrhythmia and improve defibrillation efficacy. However, the mechanism for this beneficial effect is still unclear. Since cardiac mito-chondria have been shown to play a crucial role in fatal cardiac arrhythmias and that oxidative stress is one of the main contributors to arr-hythmia generation, we tested the effects of cilostazol on cardiac mitochondria under severe oxidative stress. Methods Mitochondria were isolated from rat hearts and treated with H2O2 to induce oxidative stress. Cilostazol, at various concentrations, was used to study its protective effects. Pharmacological interventions, including a mitochondrial permeability transition pore （mPTP） blocker, cyclosporine A （CsA）, and an inner membrane anion channel （IMAC） blocker, 4＇-chlorodiazepam （CDP）, were used to investigate the mechanistic role of cilostazol on cardiac mitochondria. Cardiac mitochondrial reactive oxygen species （ROS） production, mitochondrial membrane potential change and mi-tochondrial swelling were determined as indicators of cardiac mitochondrial function. Results Cilostazol preserved cardiac mitochondrial function when exposed to oxidative stress by preventing mitochondrial depolarization, mitochondrial swelling, and decreasing ROS produc-tion. Conclusions Our findings suggest that cardioprotective effects of cilostazol reported previously could be due to its prevention of car-diac mitochondrial dysfunction caused by severe oxidative stress.展开更多
AIM： To identify the mutations of MYOC, OPTN, CYPIB1 and WDR36 in a large Chinese family affected by juvenile open angle glaucoma （JOAG）. METHODS： Of 114 members of one family were recruited in this study. Blood ...AIM： To identify the mutations of MYOC, OPTN, CYPIB1 and WDR36 in a large Chinese family affected by juvenile open angle glaucoma （JOAG）. METHODS： Of 114 members of one family were recruited in this study. Blood samples from twelve members of this pedigree were collected for further research. As a control, 100 unrelated subjects were recruited from the same hospital. The exon and flanking intron sequences of candidate genes were amplified using the polymerase chain reaction and direct DNA sequencing. RESULTS： The proband （111：10） was a seventy-three years old woman with binocular JOAG at the age of 31. A recurrent heterozygous mutation （c.1099G〉A） of MYOC was identified in the three JOAG patients and another suspect. This transition was located in the first base pair of codon 367 （GGA〉AGA） in exon 3 of MYOC and was predicted to be a missense substitution of glycine to arginine （p.G367R） in myocilin. Mutations in OPTN, CYPIB1 or WDR36 were not detected in this study. The G367R mutation was not present in unaffected family members or in 100 ethnically matched controls. Other variants of the coding regions of candidate genes were not detected in all participants. To date, this family was the largest to have been identified as carrying a certain MYOC mutation in China, further evidence of a founder effect for the G367R MYOC mutant was provided by our data. CONCLUSION： A MYOC c.1099G〉A mutation in an autosomal dominant JOAG family is identified and the characteristic phenotypes among the patients are summarized. Genetic testing could be utilized in high-risk populations and be helpful not only for genetic counseling, but also for early diagnosis and treatment of affected patients or carriers of inherited JOAG.展开更多
Animal models have been extensively used in cancer pathology studies and drug discovery.These models,however,fail to reflect the complex human tumor microenvironment and do not allow for high-throughput drug screening...Animal models have been extensively used in cancer pathology studies and drug discovery.These models,however,fail to reflect the complex human tumor microenvironment and do not allow for high-throughput drug screening in more human-like physiological conditions.Three-dimensional(3D)cancer models present an alternative to automated high-throughput cancer drug discovery and oncology.In this review,we highlight recent technology innovations in building 3D tumor models that simulate the complex human tumor microenvironment and responses of patients to treatment.We discussed various biofabrication technologies,including 3D bioprinting techniques developed for characterizing tumor progression,metastasis,and response to treatment.展开更多
Speckle noise reduction is a key problem of the image analysis of medical UltraSound images. In this paper, two important improvements have been developed to a fast anisotropic diffusion algorithm for speckle noise re...Speckle noise reduction is a key problem of the image analysis of medical UltraSound images. In this paper, two important improvements have been developed to a fast anisotropic diffusion algorithm for speckle noise reduction. The Gaussian filter is firstly used before gradient calculation, and then the adaptive algorithm of the factor k is proposed. Numerous experimental results show that the proposed model is superior to other methods in noise removal, fidelity and edge preservation. It is suitable for the preprocessing of a great number of medical UltraSound images, such as three dimen- sional reconstruction.展开更多
Chitosan-coated nylon membranes which possess a large number of reactive groups of-CH2OH and -NH2 were prepared by coupling chitosan onto the nylon membrane. Then polylysine as ligand was also immobilized onto the com...Chitosan-coated nylon membranes which possess a large number of reactive groups of-CH2OH and -NH2 were prepared by coupling chitosan onto the nylon membrane. Then polylysine as ligand was also immobilized onto the composite membranes by 1, 1＇-carbonyl-diimidazole activation to prepare affinity membranes for bilirubin adsorption. The results showed that these membranes exhibited high binding affinity capacities for bilirubin and the adsorption isotherm fitted the Freundlich model well.展开更多
Using coal tar pitch as a matrix precursor to prepare carbon materials is widelyused by impregnation/carbonization processing technology.Four different grades of coaltar pitch and a natural pitch were characterized in...Using coal tar pitch as a matrix precursor to prepare carbon materials is widelyused by impregnation/carbonization processing technology.Four different grades of coaltar pitch and a natural pitch were characterized in terms of carbon yield, density, viscosity,and fractionation with solvents, as well as by thermal analysis methods.The suitability ofthese commercially available matrices for densification of 3 dimensional carbon-carboncomposites was examined.The theoretical results compared with experimental results.The highest density after impregnation was obtained using one of the coal tar pitches.Thepredicted results are in reasonable agreement with experiment data.The significance ofthis research is that a special heat treatment regime was conducted.The effects of modificationtemperature on the densification efficiency of composites were investigated andthen structure and characteristics of the composites were determined by scanning electronmicroscopy (SEM), Transmission electron microscopy (TEM) and X-Ray Diffraction (XRD).展开更多
Rheumatic diseases, characterized by chronic inflammation and damage to various organs and systems, include systemic lupus erythematosus, rheumatoid arthritis, ankylosing spondylitis and other connective tissue diseas...Rheumatic diseases, characterized by chronic inflammation and damage to various organs and systems, include systemic lupus erythematosus, rheumatoid arthritis, ankylosing spondylitis and other connective tissue diseases. Bone is a target in many inflammatory rheumatic diseases. In recent years, the survival of patients with rheumatic diseases has increased markedly and the relationship between rheumatic diseases and osteoporosis(OP) has become more prominent. OP and related fragility fractures increase the morbidity and mortality of rheumatic disease. The cause of OP in rheumatic diseases is complex. The pathogenesis of OP in rheumatic diseases is multifactorial, including disease and treatment-related factors. Osteoimmunology, a crosstalk between inflammatory and bone cells, provides some insight into the pathogenesis of bone loss in systematic inflammatory diseases. The aim of this article is to review different risk factors in rheumatic diseases. Several factors play a role, such as chronic inflammation, immunological factors, traditional factors, metabolism and drug factors. Chronic inflammation is the most important risk factor and drug treatment is complex in patients with OP and rheumatic disease. Attention should be paid to bone loss in rheumatic disease. Optimal treatment of the underlying rheumatic disease is the first step towards prevention of OP and fractures. Apart from that, a healthy lifestyle is important as well as calcium and vitamin D supplementation. Bisphosphonates or denosumab might be necessary for patients with a low T score.展开更多
Eriocalyxin B,an ent-Kaurene diterpenoid extracted from a traditional Chinese herb Isodon eriocalyx,has been shown to possess multifunctional activities such as anti-cancer and anti-inflammatory.However,the function a...Eriocalyxin B,an ent-Kaurene diterpenoid extracted from a traditional Chinese herb Isodon eriocalyx,has been shown to possess multifunctional activities such as anti-cancer and anti-inflammatory.However,the function and mechanism of the compound in adipocyte differentiation is still unknown.Here we reported that eriocalyxin B blunted adipogenesis remarkably by inhibiting the accumulation of lipid droplets,triglycerides and the expressions of adipogenesis-related factors,including C/EBPβ,C/EBPα,PPARγ,and FABP4.Moreover,we showed that the inhibition might be the consequence of cell cycle being arrested at the G2/M phase during the mitotic clonal expansion of adipocyte differentiation,most likely by suppress-ing mRNAs and proteins of CDK1,CDK2,Cyclin A and Cyclin B1.Overall,we conclude that eriocalyxin B is capable of inhibiting adipocyte differentiation at the early stage through downregulating the proteins involved in cell cycle progression.展开更多
Artemisinin resistance in Plasmodium falciparum threatens the remarkable efficacy of artemisininbased combination therapies worldwide. Thus, greater insight into the resistance mechanism using monitoring tools is esse...Artemisinin resistance in Plasmodium falciparum threatens the remarkable efficacy of artemisininbased combination therapies worldwide. Thus, greater insight into the resistance mechanism using monitoring tools is essential. The ring-stage survival assay is used for phenotyping artemisinin-resistance or decreased artemisinin sensitivity. Here, we review the progress of this measurement assay and explore its limitations and potential applications.展开更多
An implantable optrode with micro-thermal detectors was designed to investigate the availability and safety of INS using high repetition rates.Optical auditory brainstem responses(oABRs)were recorded in normal-hearing...An implantable optrode with micro-thermal detectors was designed to investigate the availability and safety of INS using high repetition rates.Optical auditory brainstem responses(oABRs)were recorded in normal-hearing guinea pigs,and the energy thresholds,pulse durations,and ampli-tudes evoked by the varied stimulus repetitions were analyzed.Stable oABRs could be evoked through INS even as the repetition rate of stimulation reached 19 kHz.The energy threshold of oABRs was elevated,the amplitudes decreased as pulse durations increased and repetition rates were higher,and the latencies were delayed as the pulse durations increased.The temperature variation curves on the site of stimulation significantly increased as the pulse duration increased to 400μs.INS elevated the temperature around the stimulus site area via thermal accumulation during radiation,especially when higher repetition stimuli were used.Our results demonstrate that high repetition infrared stimulations can safely evoke stable and available oABRs in normal-hearing guinea pigs.展开更多
This paper deals with the temperature correlation of gray scale of B-mode ultrasound image from heated tissue. In this study, many in-vitro fresh pig livers are heated in a temperature range from 28 ℃ to 45℃, from w...This paper deals with the temperature correlation of gray scale of B-mode ultrasound image from heated tissue. In this study, many in-vitro fresh pig livers are heated in a temperature range from 28 ℃ to 45℃, from which a series of B-mode ultrasonic images of livers were obtained. The gray-value is evaluated from the ultrasound images respectively. A correlation of the mean gray value of the selected regions (12×12 pixels) in B-mode ultrasonic images of liver and its temperature was pointed out. And the experiment results agreed the evaluation well. And it is possible to monitor the tissue temperature changing in hyperthermia using this correlation.展开更多
Osteoconductive function is remarkably low in bone disease in the absence of bone tissue surrounding the grafting site,or if the bone tissue is in poor condition.Thus,an effective bone graft in terms of both osteocond...Osteoconductive function is remarkably low in bone disease in the absence of bone tissue surrounding the grafting site,or if the bone tissue is in poor condition.Thus,an effective bone graft in terms of both osteoconductivity and osteoinductivity is required for clinical therapy.Recently,the three-dimensional(3D)kagome structure has been shown to be advantageous for bone tissue regeneration due to its mechanical properties.In this study,a polycaprolactone(PCL)kagome-structure scaffold containing a hyaluronic acid(HA)-based hydrogel was fabricated using a 3D printing technique.The retention capacity of the hydrogel in the scaffold was assessed in vivo with a rat calvaria subcutaneous model for 3 weeks,and the results were compared with those obtained with conventional 3D-printed PCL grid-structure scaffolds containing HA-based hydrogel and bulk-type HA-based hydrogel.The retained hydrogel in the kagome-structure scaffold was further evaluated by in vivo imaging system analysis.To further reinforce the osteoinductivity of the kagome-structure scaffold,a PCL kagome-structure scaffold with bone morphogenetic protein-2(BMP-2)containing HA hydrogel was fabricated and implanted in a calvarial defect model of rabbits for 16 weeks.The bone regeneration characteristics were evaluated with hematoxylin and eosin(H&E),Masson’s trichrome staining,and micro-CT image analysis.展开更多
Fetal ECG extraction has the vital significance for fetal monitoring.This paper introduces a method of extracting fetal ECG based on adaptive linear neural network.The method can be realized by training a small quanti...Fetal ECG extraction has the vital significance for fetal monitoring.This paper introduces a method of extracting fetal ECG based on adaptive linear neural network.The method can be realized by training a small quantity of data.In addition,a better result can be achieved by improving neural network structure.Thus,more easily identified fetal ECG can be extracted.Experimental results show that the adaptive linear neural network can be used to extract fetal ECG from maternal abdominal signal effectively.What's more,a clearer fetal ECG can be extracted by improving neural network structure.展开更多
A method that applies clustering technique to reduce the number of samples of large data sets using input-output clustering is proposed.The proposed method clusters the output data into groups and clusters the input d...A method that applies clustering technique to reduce the number of samples of large data sets using input-output clustering is proposed.The proposed method clusters the output data into groups and clusters the input data in accordance with the groups of output data.Then,a set of prototypes are selected from the clustered input data.The inessential data can be ultimately discarded from the data set.The proposed method can reduce the effect from outliers because only the prototypes are used.This method is applied to reduce the data set in regression problems.Two standard synthetic data sets and three standard real-world data sets are used for evaluation.The root-mean-square errors are compared from support vector regression models trained with the original data sets and the corresponding instance-reduced data sets.From the experiments,the proposed method provides good results on the reduction and the reconstruction of the standard synthetic and real-world data sets.The numbers of instances of the synthetic data sets are decreased by 25%-69%.The reduction rates for the real-world data sets of the automobile miles per gallon and the 1990 census in CA are 46% and 57%,respectively.The reduction rate of 96% is very good for the electrocardiogram(ECG) data set because of the redundant and periodic nature of ECG signals.For all of the data sets,the regression results are similar to those from the corresponding original data sets.Therefore,the regression performance of the proposed method is good while only a fraction of the data is needed in the training process.展开更多
We propose a novel optical method for glucose measurement based on difuse photon-pair density wave(DPPDW)in a multiple scattering medium(MSM)where the light scattering of photon-pair is induced by refractive index mis...We propose a novel optical method for glucose measurement based on difuse photon-pair density wave(DPPDW)in a multiple scattering medium(MSM)where the light scattering of photon-pair is induced by refractive index mismatch between scatters and phantom solution.Experi-mentally,the DPPDW propagates in MSM via a two frequency laser(TFL)beam wherein highly correlated pairs of linear polarized photons are generated.The reduced scattering coefficientμ2s and absorption coefficientμ2a of DPPDW are measured simultaneously in terms of the amplitude and phase measurements of the detected heterodyne signal under arrangement at different dis-tances between the source and detection fibers in MSM.The results show that the sensitivity of glucose detection via glucose-induced change of reduced scattering coefficient(δμ′2)is 0.049%mM^(-1)in a 1%intralipid solution.In addition,the linear range ofδμ′2s vs glucose concentration implies that this DPPDW method can be used to monitor glucose concentration continuously and noninvasively subcutaneously.展开更多
Pressure ulcers are a common complication among wheelchair-bound population. They are resulted from prolonged exposure to high pressure, which restricts blood flow and leads to tissue necrosis. In this work, a continu...Pressure ulcers are a common complication among wheelchair-bound population. They are resulted from prolonged exposure to high pressure, which restricts blood flow and leads to tissue necrosis. In this work, a continuous pressure monitoring system is developed for pressure ulcer prevention. The system consists of 64 pressure sensors on a 40×50 cm2 sheet. Real time pressure data and corresponding maps are displayed on a computer simultaneously. Furthermore, a posture detection procedure is proposed for sitting posture identification. Having information about the patient’s postural history, caregivers are capable of a better decision about repositioning and treating the patient.展开更多
文摘Backgrounds: While there’s developing proof aimed toward improving embryo implantation thru a focal point on great development, restrained studies have been performed on enhancing endometrial receptivity. Intrauterine Laser Therapy (LT) can be powerful in selling endometrial cell proliferation, therefore enhancing the achievement of assisted reproductive techniques (ART). The contemporary look at aimed to research the effectiveness of effective intrauterine lasers in growing endometrial thickness and the achievement of being pregnant rate. Materials and Methods: In the current clinical randomized trial (RTC) study, the infertile women (20 - 42 years old) referred to the infertility clinic in 2023-2024 who were candidates for IVF treatment with recurrent implantation failure (RIF) history were included. The patients were divided into two main groups: the intervention group;low level laser therapy (LLLT) after hormone administration) (n = 52) and the control group (hormone administration without LT) (n = 52). The IVF success rate and change in endometrial thickness before and after the LT were compared in groups. Results: There was a significant difference between groups (p Conclusion: It appears that the incorporation of Intrauterine LT in the realm of infertility could significantly impact as a novel supplementary treatment in improving endometrial receptivity and pregnancy rate.
基金Supported by NIH,No.R01HL84590NYSTEMUniversity at Buffalo Biomedical Research Service Center
文摘Mesenchymal stem cell(MSC)therapy is entering a challenging phase after completion of many preclinical and clinical trials.Among the major hurdles encountered in MSC therapy are inconsistent stem cell potency,poor cell engraftment and survival,and age/disease-related host tissue impairment.The recognition that MSCs primarily mediate therapeutic benefits through paracrine mechanisms independent of cell differentiation provides a promising framework for enhancing stem cell potency and therapeutic benefits.Several MSC priming approaches are highlighted,which will likely allow us to harness the full potential of adult stem cells for their future routine clinical use.
基金the financial support of the National Basic Research Program of China(Grant No.2012CB619102)National High Technology Research and Development Program of China(Grant No.2011AA030103)+1 种基金National Science and Technology Support Program(Grant No.2012BAI18B01)Guangdong Innovation R&D Team Project(Grant No. 201001C0104669453)
文摘The mechanical properties, chemical properties and biocompatibility of Mg-3Sn-0.5Mn alloy were tested. A series of in vitro evaluations such as tensile test, static and dynamic immersion test, hemocompatibility test as well as cytotoxicity test were presented, with commercial magnesium alloy WE43 as the control. Mg-3Sn-0.5Mn alloy possesses suitable strength and superior ductility compared with WE43 and AZ31. Static immersion and dynamic degradation tests showed more uniform degradation with a more moderate rate for Mg-3Sn-0.5Mn alloy （0.34 mm/y in static condition and 0.25 mm/y in dynamic condition） compared with WE43 alloy （0.42 mm/y in static condition and 0.33 mm/y in dynamic condition） in Hank＇s solution. Blood compatibility evaluation suggested that Mg-3Sn-0.5Mn alloy had no destructive effect on erythrocyte and showed excellent anti-thrombogenicity to blood system. Besides, Mg-3Sn-0.5Mn alloy showed no inhibition effect to L929 metabolic activity and mild toxicity to vascular smooth muscle cell （VSMC） in preliminary cell viability assessment. By considering its excellent mechanical strength, corrosion resistance, low ion release rate and good biocompatibility, Mg-3Sn-0.5Mn alloy may be a promising economical candidate as biomedical implant material for load-bearing clinical applications in the future.
文摘Background Cilostazol is a type 3 phosphodiesterase inhibitor which has been previously demonstrated to prevent the occurrence of tachyarrhythmia and improve defibrillation efficacy. However, the mechanism for this beneficial effect is still unclear. Since cardiac mito-chondria have been shown to play a crucial role in fatal cardiac arrhythmias and that oxidative stress is one of the main contributors to arr-hythmia generation, we tested the effects of cilostazol on cardiac mitochondria under severe oxidative stress. Methods Mitochondria were isolated from rat hearts and treated with H2O2 to induce oxidative stress. Cilostazol, at various concentrations, was used to study its protective effects. Pharmacological interventions, including a mitochondrial permeability transition pore （mPTP） blocker, cyclosporine A （CsA）, and an inner membrane anion channel （IMAC） blocker, 4＇-chlorodiazepam （CDP）, were used to investigate the mechanistic role of cilostazol on cardiac mitochondria. Cardiac mitochondrial reactive oxygen species （ROS） production, mitochondrial membrane potential change and mi-tochondrial swelling were determined as indicators of cardiac mitochondrial function. Results Cilostazol preserved cardiac mitochondrial function when exposed to oxidative stress by preventing mitochondrial depolarization, mitochondrial swelling, and decreasing ROS produc-tion. Conclusions Our findings suggest that cardioprotective effects of cilostazol reported previously could be due to its prevention of car-diac mitochondrial dysfunction caused by severe oxidative stress.
基金Supported by Natural Science Foundation of China(No.81270999No.81570870)Professor Academic Development Fund of Fujian Medical University(No.JS14019)
文摘AIM： To identify the mutations of MYOC, OPTN, CYPIB1 and WDR36 in a large Chinese family affected by juvenile open angle glaucoma （JOAG）. METHODS： Of 114 members of one family were recruited in this study. Blood samples from twelve members of this pedigree were collected for further research. As a control, 100 unrelated subjects were recruited from the same hospital. The exon and flanking intron sequences of candidate genes were amplified using the polymerase chain reaction and direct DNA sequencing. RESULTS： The proband （111：10） was a seventy-three years old woman with binocular JOAG at the age of 31. A recurrent heterozygous mutation （c.1099G〉A） of MYOC was identified in the three JOAG patients and another suspect. This transition was located in the first base pair of codon 367 （GGA〉AGA） in exon 3 of MYOC and was predicted to be a missense substitution of glycine to arginine （p.G367R） in myocilin. Mutations in OPTN, CYPIB1 or WDR36 were not detected in this study. The G367R mutation was not present in unaffected family members or in 100 ethnically matched controls. Other variants of the coding regions of candidate genes were not detected in all participants. To date, this family was the largest to have been identified as carrying a certain MYOC mutation in China, further evidence of a founder effect for the G367R MYOC mutant was provided by our data. CONCLUSION： A MYOC c.1099G〉A mutation in an autosomal dominant JOAG family is identified and the characteristic phenotypes among the patients are summarized. Genetic testing could be utilized in high-risk populations and be helpful not only for genetic counseling, but also for early diagnosis and treatment of affected patients or carriers of inherited JOAG.
文摘Animal models have been extensively used in cancer pathology studies and drug discovery.These models,however,fail to reflect the complex human tumor microenvironment and do not allow for high-throughput drug screening in more human-like physiological conditions.Three-dimensional(3D)cancer models present an alternative to automated high-throughput cancer drug discovery and oncology.In this review,we highlight recent technology innovations in building 3D tumor models that simulate the complex human tumor microenvironment and responses of patients to treatment.We discussed various biofabrication technologies,including 3D bioprinting techniques developed for characterizing tumor progression,metastasis,and response to treatment.
基金Supported by National Natural Science Foundation of China (No.60272060)Doctoral Foundation of Ministry of Education (No.20030610032)Sichuan Youth Science and Technology Foundation (No.04ZQ026-013).
文摘Speckle noise reduction is a key problem of the image analysis of medical UltraSound images. In this paper, two important improvements have been developed to a fast anisotropic diffusion algorithm for speckle noise reduction. The Gaussian filter is firstly used before gradient calculation, and then the adaptive algorithm of the factor k is proposed. Numerous experimental results show that the proposed model is superior to other methods in noise removal, fidelity and edge preservation. It is suitable for the preprocessing of a great number of medical UltraSound images, such as three dimen- sional reconstruction.
基金We are extremely grateful to the National Natural Science Foundation of China for supporting this research(No.29776036).
文摘Chitosan-coated nylon membranes which possess a large number of reactive groups of-CH2OH and -NH2 were prepared by coupling chitosan onto the nylon membrane. Then polylysine as ligand was also immobilized onto the composite membranes by 1, 1＇-carbonyl-diimidazole activation to prepare affinity membranes for bilirubin adsorption. The results showed that these membranes exhibited high binding affinity capacities for bilirubin and the adsorption isotherm fitted the Freundlich model well.
文摘Using coal tar pitch as a matrix precursor to prepare carbon materials is widelyused by impregnation/carbonization processing technology.Four different grades of coaltar pitch and a natural pitch were characterized in terms of carbon yield, density, viscosity,and fractionation with solvents, as well as by thermal analysis methods.The suitability ofthese commercially available matrices for densification of 3 dimensional carbon-carboncomposites was examined.The theoretical results compared with experimental results.The highest density after impregnation was obtained using one of the coal tar pitches.Thepredicted results are in reasonable agreement with experiment data.The significance ofthis research is that a special heat treatment regime was conducted.The effects of modificationtemperature on the densification efficiency of composites were investigated andthen structure and characteristics of the composites were determined by scanning electronmicroscopy (SEM), Transmission electron microscopy (TEM) and X-Ray Diffraction (XRD).
文摘Rheumatic diseases, characterized by chronic inflammation and damage to various organs and systems, include systemic lupus erythematosus, rheumatoid arthritis, ankylosing spondylitis and other connective tissue diseases. Bone is a target in many inflammatory rheumatic diseases. In recent years, the survival of patients with rheumatic diseases has increased markedly and the relationship between rheumatic diseases and osteoporosis(OP) has become more prominent. OP and related fragility fractures increase the morbidity and mortality of rheumatic disease. The cause of OP in rheumatic diseases is complex. The pathogenesis of OP in rheumatic diseases is multifactorial, including disease and treatment-related factors. Osteoimmunology, a crosstalk between inflammatory and bone cells, provides some insight into the pathogenesis of bone loss in systematic inflammatory diseases. The aim of this article is to review different risk factors in rheumatic diseases. Several factors play a role, such as chronic inflammation, immunological factors, traditional factors, metabolism and drug factors. Chronic inflammation is the most important risk factor and drug treatment is complex in patients with OP and rheumatic disease. Attention should be paid to bone loss in rheumatic disease. Optimal treatment of the underlying rheumatic disease is the first step towards prevention of OP and fractures. Apart from that, a healthy lifestyle is important as well as calcium and vitamin D supplementation. Bisphosphonates or denosumab might be necessary for patients with a low T score.
基金supported by National Key R&D Program of China,(2017YFC1700906)Yunnan Provincial Science and Technology Department,China(2017FA044 and 2013HA023).
文摘Eriocalyxin B,an ent-Kaurene diterpenoid extracted from a traditional Chinese herb Isodon eriocalyx,has been shown to possess multifunctional activities such as anti-cancer and anti-inflammatory.However,the function and mechanism of the compound in adipocyte differentiation is still unknown.Here we reported that eriocalyxin B blunted adipogenesis remarkably by inhibiting the accumulation of lipid droplets,triglycerides and the expressions of adipogenesis-related factors,including C/EBPβ,C/EBPα,PPARγ,and FABP4.Moreover,we showed that the inhibition might be the consequence of cell cycle being arrested at the G2/M phase during the mitotic clonal expansion of adipocyte differentiation,most likely by suppress-ing mRNAs and proteins of CDK1,CDK2,Cyclin A and Cyclin B1.Overall,we conclude that eriocalyxin B is capable of inhibiting adipocyte differentiation at the early stage through downregulating the proteins involved in cell cycle progression.
基金funded by the National Natural Science Foundation of China(31260508,U1202226)grants of Doctor Newcomer Scholarship from Yunnan province
文摘Artemisinin resistance in Plasmodium falciparum threatens the remarkable efficacy of artemisininbased combination therapies worldwide. Thus, greater insight into the resistance mechanism using monitoring tools is essential. The ring-stage survival assay is used for phenotyping artemisinin-resistance or decreased artemisinin sensitivity. Here, we review the progress of this measurement assay and explore its limitations and potential applications.
基金This work was supported by grants from the National Natural Science Foundation of China(81660173)the Natural Science Foundation of Jiangxi Province(20202BABL206065)the Key Research and Development Program of Jiangxi Province(20181BBG78013).
文摘An implantable optrode with micro-thermal detectors was designed to investigate the availability and safety of INS using high repetition rates.Optical auditory brainstem responses(oABRs)were recorded in normal-hearing guinea pigs,and the energy thresholds,pulse durations,and ampli-tudes evoked by the varied stimulus repetitions were analyzed.Stable oABRs could be evoked through INS even as the repetition rate of stimulation reached 19 kHz.The energy threshold of oABRs was elevated,the amplitudes decreased as pulse durations increased and repetition rates were higher,and the latencies were delayed as the pulse durations increased.The temperature variation curves on the site of stimulation significantly increased as the pulse duration increased to 400μs.INS elevated the temperature around the stimulus site area via thermal accumulation during radiation,especially when higher repetition stimuli were used.Our results demonstrate that high repetition infrared stimulations can safely evoke stable and available oABRs in normal-hearing guinea pigs.
基金The research was supported by National Nature Science Foundation (30470450) Education Committee Foundation( KP0608200201 ) Elitist Foundation( KW5800200351 ) from Beijing City,China.
文摘This paper deals with the temperature correlation of gray scale of B-mode ultrasound image from heated tissue. In this study, many in-vitro fresh pig livers are heated in a temperature range from 28 ℃ to 45℃, from which a series of B-mode ultrasonic images of livers were obtained. The gray-value is evaluated from the ultrasound images respectively. A correlation of the mean gray value of the selected regions (12×12 pixels) in B-mode ultrasonic images of liver and its temperature was pointed out. And the experiment results agreed the evaluation well. And it is possible to monitor the tissue temperature changing in hyperthermia using this correlation.
基金supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute(KHIDI),the Ministry of Health&Welfare,Republic of Korea(Grant Number:HI14C2143)the National Research Foundation of Korea(NRF)grant funded by the Korea government(MIST)(NRF-2021R1A2C2009665)。
文摘Osteoconductive function is remarkably low in bone disease in the absence of bone tissue surrounding the grafting site,or if the bone tissue is in poor condition.Thus,an effective bone graft in terms of both osteoconductivity and osteoinductivity is required for clinical therapy.Recently,the three-dimensional(3D)kagome structure has been shown to be advantageous for bone tissue regeneration due to its mechanical properties.In this study,a polycaprolactone(PCL)kagome-structure scaffold containing a hyaluronic acid(HA)-based hydrogel was fabricated using a 3D printing technique.The retention capacity of the hydrogel in the scaffold was assessed in vivo with a rat calvaria subcutaneous model for 3 weeks,and the results were compared with those obtained with conventional 3D-printed PCL grid-structure scaffolds containing HA-based hydrogel and bulk-type HA-based hydrogel.The retained hydrogel in the kagome-structure scaffold was further evaluated by in vivo imaging system analysis.To further reinforce the osteoinductivity of the kagome-structure scaffold,a PCL kagome-structure scaffold with bone morphogenetic protein-2(BMP-2)containing HA hydrogel was fabricated and implanted in a calvarial defect model of rabbits for 16 weeks.The bone regeneration characteristics were evaluated with hematoxylin and eosin(H&E),Masson’s trichrome staining,and micro-CT image analysis.
基金Foundation of Young Backbone Teacher of Beijing Citygrant number:102KB000845
文摘Fetal ECG extraction has the vital significance for fetal monitoring.This paper introduces a method of extracting fetal ECG based on adaptive linear neural network.The method can be realized by training a small quantity of data.In addition,a better result can be achieved by improving neural network structure.Thus,more easily identified fetal ECG can be extracted.Experimental results show that the adaptive linear neural network can be used to extract fetal ECG from maternal abdominal signal effectively.What's more,a clearer fetal ECG can be extracted by improving neural network structure.
基金supported by Chiang Mai University Research Fund under the contract number T-M5744
文摘A method that applies clustering technique to reduce the number of samples of large data sets using input-output clustering is proposed.The proposed method clusters the output data into groups and clusters the input data in accordance with the groups of output data.Then,a set of prototypes are selected from the clustered input data.The inessential data can be ultimately discarded from the data set.The proposed method can reduce the effect from outliers because only the prototypes are used.This method is applied to reduce the data set in regression problems.Two standard synthetic data sets and three standard real-world data sets are used for evaluation.The root-mean-square errors are compared from support vector regression models trained with the original data sets and the corresponding instance-reduced data sets.From the experiments,the proposed method provides good results on the reduction and the reconstruction of the standard synthetic and real-world data sets.The numbers of instances of the synthetic data sets are decreased by 25%-69%.The reduction rates for the real-world data sets of the automobile miles per gallon and the 1990 census in CA are 46% and 57%,respectively.The reduction rate of 96% is very good for the electrocardiogram(ECG) data set because of the redundant and periodic nature of ECG signals.For all of the data sets,the regression results are similar to those from the corresponding original data sets.Therefore,the regression performance of the proposed method is good while only a fraction of the data is needed in the training process.
文摘We propose a novel optical method for glucose measurement based on difuse photon-pair density wave(DPPDW)in a multiple scattering medium(MSM)where the light scattering of photon-pair is induced by refractive index mismatch between scatters and phantom solution.Experi-mentally,the DPPDW propagates in MSM via a two frequency laser(TFL)beam wherein highly correlated pairs of linear polarized photons are generated.The reduced scattering coefficientμ2s and absorption coefficientμ2a of DPPDW are measured simultaneously in terms of the amplitude and phase measurements of the detected heterodyne signal under arrangement at different dis-tances between the source and detection fibers in MSM.The results show that the sensitivity of glucose detection via glucose-induced change of reduced scattering coefficient(δμ′2)is 0.049%mM^(-1)in a 1%intralipid solution.In addition,the linear range ofδμ′2s vs glucose concentration implies that this DPPDW method can be used to monitor glucose concentration continuously and noninvasively subcutaneously.
文摘Pressure ulcers are a common complication among wheelchair-bound population. They are resulted from prolonged exposure to high pressure, which restricts blood flow and leads to tissue necrosis. In this work, a continuous pressure monitoring system is developed for pressure ulcer prevention. The system consists of 64 pressure sensors on a 40×50 cm2 sheet. Real time pressure data and corresponding maps are displayed on a computer simultaneously. Furthermore, a posture detection procedure is proposed for sitting posture identification. Having information about the patient’s postural history, caregivers are capable of a better decision about repositioning and treating the patient.
