The ability to noninvasively manipulate and isolate specific cell populations in vivo is critical for advancing real-time diagnostics,precision medicine,and immunological research.Here,we present a novel and broadly a...The ability to noninvasively manipulate and isolate specific cell populations in vivo is critical for advancing real-time diagnostics,precision medicine,and immunological research.Here,we present a novel and broadly applicable optical trapping system based on a custom-designed 2×3 optical tweezer array,which enables the real-time interception and manipulation of circulating leukocytes in live animals.By utilizing intrinsic velocity differences between leukocytes and red blood cells,the system achieves stable trapping of individual leukocytes in vessels 15-20μm in diameter and decelerates multiple cells in vessels greater than 20μm.Notably,it also enables the optical blockage of lymphatic vessels exceeding 50μm,a previously unreported capability.This label-free,noninvasive approach operates without repeated blood draws and is compatible with diverse vessel geometries and flow dynamics.The system offers a generalizable solution for in vivo cell extraction and analysis,paving the way for high-precision single-cell technologies in biomedical research and clinical translation.展开更多
Gastrointestinal(GI)cancers remain a leading cause of cancer-related morbidity and mortality worldwide.Artificial intelligence(AI),particularly machine learning and deep learning(DL),has shown promise in enhancing can...Gastrointestinal(GI)cancers remain a leading cause of cancer-related morbidity and mortality worldwide.Artificial intelligence(AI),particularly machine learning and deep learning(DL),has shown promise in enhancing cancer detection,diagnosis,and prognostication.A narrative review of literature published from January 2015 to march 2025 was conducted using PubMed,Web of Science,and Scopus.Search terms included"gastrointestinal cancer","artificial intelligence","machine learning","deep learning","radiomics","multimodal detection"and"predictive modeling".Studies were included if they focused on clinically relevant AI applications in GI oncology.AI algorithms for GI cancer detection have achieved high performance across imaging modalities,with endoscopic DL systems reporting accuracies of 85%-97%for polyp detection and segmentation.Radiomics-based models have predicted molecular biomarkers such as programmed cell death ligand 2 expression with area under the curves up to 0.92.Large language models applied to radiology reports demonstrated diagnostic accuracy comparable to junior radiologists(78.9%vs 80.0%),though without incremental value when combined with human interpretation.Multimodal AI approaches integrating imaging,pathology,and clinical data show emerging potential for precision oncology.AI in GI oncology has reached clinically relevant accuracy levels in multiple diagnostic tasks,with multimodal approaches and predictive biomarker modeling offering new opportunities for personalized care.However,broader validation,integration into clinical workflows,and attention to ethical,legal,and social implications remain critical for widespread adoption.展开更多
Efficient strategies to promote microvascularization in vascular tissue engineering,a central priority in regenerative medicine,are still scarce;nano-and micro-sized aggregates and spheres or beads harboring primitive...Efficient strategies to promote microvascularization in vascular tissue engineering,a central priority in regenerative medicine,are still scarce;nano-and micro-sized aggregates and spheres or beads harboring primitive microvascular beds are promising methods in vascular tissue engineering.Capillaries are the smallest type and in numerous blood vessels,which are distributed densely in cardiovascular system.To mimic this microvascular network,specific cell components and proangiogenic factors are required.Herein,advanced biofabrication methods in microvascular engineering,including extrusion-based and droplet-based bioprinting,Kenzan,and biogripper approaches,are deliberated with emphasis on the newest works in prevascular nano-and micro-sized aggregates and microspheres/microbeads.展开更多
A PVA-GAG-COL composite scaffold is fabricated by polyvinyl alcohol （PVA）, glyeosaminoglycan （GAG） and collagen （COL）. Laser surface modification technology is used to make holes on the surface of the scaffolds....A PVA-GAG-COL composite scaffold is fabricated by polyvinyl alcohol （PVA）, glyeosaminoglycan （GAG） and collagen （COL）. Laser surface modification technology is used to make holes on the surface of the scaffolds. Inside and outside interconnection micro-porous structure is obtained. Bioeompatibility test of the scaffolds shows that PVA-GAG-COL scaffold can promote the adhesion and proliferation of the fibroblast. Also, fibroblast can grow normally on the scaffolds with pore diameter from 115 um to 255 um and pore distance from 500 um to 2000 um. PVA-GAG-COL scaffolds possess excellent cell biocompatibility. The porous structure is suitable for cell culture in tissue engineering.展开更多
The brain exhibits complex physiology characterized by unique features such as a brain-specific extracellular matrix, compartmentalized structure (white and grey matter), and an aligned axonal network. These physiolog...The brain exhibits complex physiology characterized by unique features such as a brain-specific extracellular matrix, compartmentalized structure (white and grey matter), and an aligned axonal network. These physiological characteristics underpin brain function and facilitate signal transduction similar to that in an electrical circuit. Therefore, investigating these features in vitro is crucial for understanding the interactions between neuronal signal transduction processes and the pathology of neurological diseases. Compared to neurons on patterned substrates, three-dimensional (3D) bioprinting-based neural models provide significant advantages in replicating axonal kinetics without physical limitations. This study proposes the development of a 3D bioprinted engineered neural network (BENN) model to replicate the physiological features of the brain, suggesting its application as a tool for studying neurodegenerative diseases. We employed 3D bioprinting to reconstruct the compartmentalized structure of the brain, and controlled the directionality of axonal growth by applying electrical stimuli to the printed neural structure for overcoming spatial constraints. The reconstructed axonal network demonstrated reliability as a neural analog, including the visualization of mature neuronal features and spontaneous calcium reactions. Furthermore, these brain-like neural network models have demonstrated usefulness for studying neurodegeneration by enabling the visualization of degenerative pathophysiology in alcohol-exposed neurons. The BENN facilitates the visualization of region-specific pathological markers in soma or axon populations, including amyloid-beta formation and axonal deformation. Overall, the BENN closely mimics brain physiology, offers insights into the dynamics of axonal networks, and can be applied to studying neurological diseases.展开更多
Background:Endoplasmic reticulum(ER)stress is an important factor in the development of numerous cardiovascular disorders;nevertheless,the association between ER stress and mitral regurgitation(MR)remains inadequately...Background:Endoplasmic reticulum(ER)stress is an important factor in the development of numerous cardiovascular disorders;nevertheless,the association between ER stress and mitral regurgitation(MR)remains inadequately characterized.The molecular mechanism of pimobendan(PIMO)that contributes to the delay in congestive heart failure(CHF)in MR associated with apoptosis and fibrosis is still unclear.Our aim was to examine the impact of PIMO on ER stress,apoptosis,and fibrosis in a chronic MR rat model.Methods:MR was surgically induced in 10 Sprague–Dawley rats,with 5 serving as sham operation controls.At 8 weeks postsurgery,the MR animals were randomly allocated into two groups:MR and MR+PIMO groups.PIMO was administered twice daily through oral gavage for 4 weeks,whereas the sham and MR groups were administered similar quantities of drinking water.Echocardiography was conducted before the delivery of PIMO as a baseline measure and at the end of the study.At the end of the investigation,hearts were procured for histopathological and ER stress evaluations.Results:PIMO significantly maintained heart function and structural remodeling in the MR animals.PIMO significantly reduced MR-induced myocyte apoptosis(p=0.044)and fibrosis(p=0.002)by reducing the messenger RNA expression of genes associated with ER stress(GRP78[glucose-regulated protein 78],ATF4[activating transcription factor 4],and CHOP[C/ERP homologous protein])compared to the MR group(p<0.05,p<0.01,and p<0.001,respectively).Conclusion:PIMO demonstrated cardioprotective benefits on heart function,myocyte apoptosis,and fibrosis by regulating ER stress in an MR-induced CHF rat model.展开更多
Triple-negative bresst canær(TNBC)metastscis is particularly severe due to its aggressive nsture,leading to rapid disease progresion and significantly reduced survival rates.Rujifang(RJF),a traditional Chinese fo...Triple-negative bresst canær(TNBC)metastscis is particularly severe due to its aggressive nsture,leading to rapid disease progresion and significantly reduced survival rates.Rujifang(RJF),a traditional Chinese formula,has demonstrated potential anti-tumor effects and theability to inhibit TNBC metastasis.However,the efects af varying R.IF dors remain undear.This study utilized Laser-based in vino fow cytometry(IVFC)to monitor circulating tumor cells(CTCs)and evaluate the efficacy of R.IF at different doses.The results indicated that R.IF at the high dose inhibited both the number af CTC:and the formaton of metatatic foci more eflectively compared to the lower dose.TUNEL assays revealed that R.IF trentment promotes apoptosis of tumor cells,with a more pronounced effect observed at the higher dose.Immuno-fluorescence experiments demonstrated that administering a higher dose of R.IF suppreses theеxprescion of Kindlin-1 more effectively in the tumor microenvironment.Although higher doses showed enhanced efficacy,they might also lesd to an increase in side efects.These findings underscore the promise and challenges of using R.IF at high doses for anti-tumor therspy.They highlight the criticnl importance of optimizing the dose of R.JP in the treatment of TNBC and provide valuable insights for its dinical application.展开更多
Background:Observing interstitial fluid(ISF)is very difficult because interstitial structure collapses and ISF disappears after tissue fixation.Additionally,ISF is colorless,and interstitial flow is weak in vivo.In or...Background:Observing interstitial fluid(ISF)is very difficult because interstitial structure collapses and ISF disappears after tissue fixation.Additionally,ISF is colorless,and interstitial flow is weak in vivo.In order to view the interstitial flow,special dye and animal model was chosen to explore the movement characteristic of interstitial flow and related structure.Methods:The Gephyrocharax Melanocheir(GM)fish,a special animal with translucent body,were placed into 0.03 g/L tricain solution for anesthesia.20-25μL Alcian blue(AB)solution which can stain acid mucopolysaccharide immobilized by the collagen net in connective tissue was injected into each fish at a single point with a rate of 2 μL/min via a micro-injection pump.The process of infusion and the movement of the AB in fish were record by a digital camera.The(fresh)frozen sections of AB tracks tissue were performed to observe the morphological feature.Results:Several blue tracks were observed which were formed by longitudinal directional movements of AB solution.For back lateral track,the velocity and length of the movements were significantly fast and longer on the direction toward head than that toward tail(P<0.01).For lateral middle track,the result was opposite,namely toward tail(P<0.01).This phenomenon indicated an inherent ISF flow according to Darcy's law.Morphological study showed these tracks were just in septa composed by connective tissue.The stained blue septa formed various shapes as interstitial space for ISF flow and connected with one another like a net.The finding can help us to understand the essence of meridian in traditional Chinese medicine(TCM).Conclusions:The dynamic asymmetry of the AB tracks revealed interstitial flow in the GM fish body.It implied an inherent interstitial flow along particular pathway formed by septa which may be a key to understand the nature and value of meridians and collaterals in health care.展开更多
Prostaglandin E2(PGE2), a major cyclooxygenase-2(COX-2) product, is highly secreted by the osteoblast lineage in the subchondral bone tissue of osteoarthritis(OA) patients. However, NSAIDs, including COX-2 inhibitors,...Prostaglandin E2(PGE2), a major cyclooxygenase-2(COX-2) product, is highly secreted by the osteoblast lineage in the subchondral bone tissue of osteoarthritis(OA) patients. However, NSAIDs, including COX-2 inhibitors, have severe side effects during OA treatment. Therefore, the identification of novel drug targets of PGE2 signaling in OA progression is urgently needed. Osteoclasts play a critical role in subchondral bone homeostasis and OA-related pain. However, the mechanisms by which PGE2 regulates osteoclast function and subsequently subchondral bone homeostasis are largely unknown. Here, we show that PGE2 acts via EP4 receptors on osteoclasts during the progression of OA and OA-related pain. Our data show that while PGE2 mediates migration and osteoclastogenesis via its EP2 and EP4 receptors, tissue-specific knockout of only the EP4 receptor in osteoclasts(EP4 Lys M) reduced disease progression and osteophyte formation in a murine model of OA. Furthermore, OA-related pain was alleviated in the EP4 Lys M mice, with reduced Netrin-1 secretion and CGRP-positive sensory innervation of the subchondral bone. The expression of plateletderived growth factor-BB(PDGF-BB) was also lower in the EP4 Lys Mmice, which resulted in reduced type H blood vessel formation in subchondral bone. Importantly, we identified a novel potent EP4 antagonist, HL-43, which showed in vitro and in vivo effects consistent with those observed in the EP4 Lys Mmice. Finally, we showed that the Gαs/PI3 K/AKT/MAPK signaling pathway is downstream of EP4 activation via PGE2 in osteoclasts. Together, our data demonstrate that PGE2/EP4 signaling in osteoclasts mediates angiogenesis and sensory neuron innervation in subchondral bone, promoting OA progression and pain, and that inhibition of EP4 with HL-43 has therapeutic potential in OA.展开更多
AIM:To Introduce a new specialized visual acuity chart for amblyopic children aged 3-5 years old and its clinical applications.METHODS:The new visual acuity chart and notations were designed based on Weber-Fechner law...AIM:To Introduce a new specialized visual acuity chart for amblyopic children aged 3-5 years old and its clinical applications.METHODS:The new visual acuity chart and notations were designed based on Weber-Fechner law.The optotypes were red against a white background and were specially shaped four basic geometric symbols:circle,square,triangle,and cross.A regular geometric progression of the optotype sizes and distribution was employed to arrange in 14 lines.The progression rate of the optotype size between two lines was 1.2589 and the testing distance was 3m.Visual acuity score could be recorded as logMAR notation or decimal notation.Agestratified diagnostic criteria for amblyopia established by consensus statement on diagnosis of amblyopia(2011)among members of the Strabismus and Pediatric Ophthalmology Group,Ophthalmology Society,Chinese Medical Association(SPOGOSCMA)were illustrated in the new visual acuity chart.RESULTS:When assessing visual acuity in children aged 3-5 years old,this new visual acuity chart that consists of four symmetrical shapes(triangle,square,cross,and circle)overcame an inability to recognize the letters of the alphabet and difficulties in designating the direction of black abstract symbols such as the tumbling’E’or Landolt’C’,which the subjects were prone to lose interest in.The visual acuity score may be recorded indifferent notations:decimal acuity and logMAR.These two notations can be easily converted each other in the new eye chart.The measurements of this new chart not only showed a significant correlation and a good consistency with the international standard logarithmic visual acuity chart(r=0.932,P【0.01),but also indicated a high test-retest reliability(89%of retest scores were within 0.1 logMAR units of the initial test score).CONCLUSION:The results of this study support the validity and reliability of distance visual acuity measurements using the new eye chart in children aged3 to 5 years over a wide range of visual acuities,and the new eye chart is great for early detection of amblyopia.It can be applied in various clinical settings.展开更多
Transplantation of neural stem cells has been reported as a possible approach for replacing impaired dopaminergic neurons. In this study, we tested the efficacy of early-stage human dental papilla-derived stem cells a...Transplantation of neural stem cells has been reported as a possible approach for replacing impaired dopaminergic neurons. In this study, we tested the efficacy of early-stage human dental papilla-derived stem cells and human brain-derived neural stem cells in rat models of 6-hydroxydopamine-induced Parkinson＇s disease. Rats received a unilateral injection of 6-hydroxydopamine into right medial forebrain bundle, followed 3 weeks later by injections of PBS, early-stage human dental papilla-derived stem cells, or human brain-derived neural stem cells into the ipsilateral striatum. All of the rats in the human dental papilla-derived stem cell group died from tumor formation at around 2 weeks following cell transplantation. Postmortem examinations revealed homogeneous malignant tumors in the striatum of the human dental papilla-derived stem cell group. Stepping tests revealed that human brain-derived neural stem cell transplantation did not improve motor dysfunction. In apomorphine-induced rotation tests, neither the human brain-derived neural stem cell group nor the control groups （PBS injection） demonstrated significant changes. Glucose metabolism in the lesioned side of striatum was reduced by human brain-derived neural stem cell transplantation. [18F]-FP-CIT PET scans in the striatum did not demonstrate a significant increase in the human brain-derived neural stem cell group. Tyrosine hydroxylase （dopaminergic neuronal marker） staining and G protein-activated inward rectifier potassium channel 2 （A9 dopaminergic neuronal marker） were positive in the lesioned side of striatum in the human brain-derived neural stem cell group. The use of early-stage human dental papilla-derived stern cells confirmed its tendency to form tumors. Human brain-derived neural stem cells could be partially differentiated into dopaminergic neurons, but they did not secrete dopamine.展开更多
The subtle color distinction is the important function of electronic endoscope imaging diagnosis.However,after image acquisition,transmission and display,color distortions of intracorporeal organs or tissues occur ine...The subtle color distinction is the important function of electronic endoscope imaging diagnosis.However,after image acquisition,transmission and display,color distortions of intracorporeal organs or tissues occur inevitably,which are adverse to analyze image features accurately or to diagnose early pathological changes.A real-time color correction algorithm based on fourneighborhood and polynomial regression in YUV color space is proposed.Based on polynomial regression the color correction matrix is calculated in YUV color space according to the dierences between standard values of color checker and measured values of that imaged by the endoscope.As the correction is only executed on U and V components in YUV color space,the defect that the color of corrected images in RGB color space will change along with luminance can be avoided,and then the stability of image color is improved.Owing to four-neighborhood processing,the signal-to-noise ratio of corrected images is enhanced and the processing speed of correction algorithm is accelerated.The average color dierence is reduced from 0.3944 to 0.2850 by application of the proposed algorithm in high-denition electronic endoscope.A total of 17 frames per second can be achieved at the resolution of 1280800,and the color characteristics of the image after processing match that of human visual system.展开更多
BACKGROUND Degree of portal hypertension(PH)is the most important prognostic factor for the decompensation of liver cirrhosis and death,therefore adequate care for patients with liver cirrhosis requires timely detecti...BACKGROUND Degree of portal hypertension(PH)is the most important prognostic factor for the decompensation of liver cirrhosis and death,therefore adequate care for patients with liver cirrhosis requires timely detection and evaluation of the presence of clinically significant PH(CSPH)and severe PH(SPH).As the most accurate method for the assessment of PH is an invasive direct measurement of hepatic venous pressure gradient(HVPG),the search for non-invasive methods to diagnose these conditions is actively ongoing.AIM To evaluate the feasibility of parameters of endogenously induced displacements and strain of liver to assess degree of PH.METHODS Of 36 patients with liver cirrhosis and measured HVPG were included in the casecontrol study.Endogenous motion of the liver was characterized by derived parameters of region average tissue displacement signal(dantero,dretro,dRMS)and results of endogenous tissue strain imaging using specific radiofrequency signal processing algorithm.Average endogenous strainμand standard deviationσof strain were assessed in the regions of interest(ROI)(1 cm×1 cm and 2 cm×2 cm in size)and different frequency subbands of endogenous motion(0-10 Hz and 10-20 Hz).RESULTS Four parameters showed statistically significant(P<0.05)correlation with HVPG measurement.The strongest correlation was obtained for the standard deviation of strain(estimated at 0-10 Hz and 2 cm×2 cm ROI size).Three parameters showed statistically significant differences between patient groups with CSPH,but only dretro showed significant results in SPH analysis.According to ROC analysis area under the curve(AUC)of theσROI[0…10Hz,2 cm×2 cm]parameter reached 0.71(P=0.036)for the diagnosis of CSPH;with a cut-off value of 1.28μm/cm providing 73%sensitivity and 70%specificity.AUC for the diagnosis of CSPH forμROI[0…10Hz,1 cm×1 cm]was 0.78(P=0.0024);with a cut-off value of 3.92μm/cm providing 73%sensitivity and 80%specificity.Dretro parameter had an AUC of 0.86(P=0.0001)for the diagnosis of CSPH and 0.84(P=0.0001)for the diagnosis of SPH.A cut-off value of-132.34μm yielded 100%sensitivity for both conditions,whereas specificity was 80%and 72%for CSPH and SPH respectively.CONCLUSION The parameters of endogenously induced displacements and strain of the liver correlated with HVPG and might be used for non-invasive diagnosis of PH.展开更多
Objective：To generate recombinant adenovirus that could simultaneously express ornithine decarboxylase（ODC） and S-adenosylmethionine decarboxylase（AdoMetDC） antisenses specifically in prostate cancer cells,and ev...Objective：To generate recombinant adenovirus that could simultaneously express ornithine decarboxylase（ODC） and S-adenosylmethionine decarboxylase（AdoMetDC） antisenses specifically in prostate cancer cells,and evaluate its inhibitory effect on prostate cancer in vivo.Methods：Fragments of ODC and AdoMetDC genes were generated by PCR,cloned into the pPGL-PSES,and then recombined with pAdEasy-1 vectors in AdEasy-1 cells.Ad-PSES-ODC-AdoMetDCas virus was produced in HEK293 cells.Following transfection with Ad-PSES-ODC-AdoMetDCas,the levels of ODC or AdoMetDC were determined by RT-PCR and western blot assays.The effect of Ad-PSES-ODC-AdoMetDCas treatment on tumor formation and growth was evaluated in xenograft models of prostate cancers in vivo.Results：The plasmid pAdEasy-PSES-ODC-AdoMetDCas was successfully constructed and the recombinant Ad-PSES-ODC-AdoMetDCas adenovirus was produced.Transfection with Ad-PSES-ODC-AdoMetDCas adenovirus significantly inhibited the expression of ODC and AdoMetDC genes specifically in prostate DU145 cells,but not H1299,HT29 and HepG2 cancer cells,and disrupted the ability of DU145 cells to form solid prostate cancer in vivo.Intratumoral treatment with Ad-PSES-ODC-AdoMetDCas adenovirus significantly inhibited the growth of engrafted prostate tumors in vivo.Conclusion：The recombinant Ad-PSES-ODC-AdoMetDCas adenovirus specifically reduces the expression of both ODC and AdoMetDC genes in prostate cells and may be used for treatment of prostate cancers at the clinic.展开更多
BACKGROUND Lumbar disc herniation(LDH)has emerged as one of the most common causes of low back pain.The routine treatment approach involves chemonucleolysis therapy,discectomy by percutaneous endoscopy,and percutaneou...BACKGROUND Lumbar disc herniation(LDH)has emerged as one of the most common causes of low back pain.The routine treatment approach involves chemonucleolysis therapy,discectomy by percutaneous endoscopy,and percutaneous laser disc decompression.Unfortunately,all of these methods carry inherent risk of causing harm to the patient and,as such,there is an unmet but urgent need for an effective and safe noninvasive treatment for LDH.The purpose of this report is to describe a non-invasive method for re-absorption of LDH.CASE SUMMARY A 34-year-old woman was admitted with a complaint of waist pain that she reported as having become acutely aggravated over the past 3 d and accompanied by discomfort in the right lower limb.Her self-reported medical history included persistent postpartum low back pain from 7 years prior.Physical exam showed positivity for neck flexion test(Lindner sign)and supine abdomen test;the straight leg-raising test showed right 60(+)and left 80(-).Findings from standard imaging(magnetic resonance)and collective physical examinations indicated a L5/S1 herniated lumbar disc.Treatment consisted of three-dimensional(balanced regulating)spinal manipulation and acupuncture,upon which the LDH resolved by retraction.CONCLUSION Following L5/S1 herniated lumbar disc diagnosis,three-dimensional(balanced regulating)spinal manipulation combined with acupuncture therapy is an effective treatment.展开更多
AIM:To introduce a new near-vision chart for children aged 3-5 years old and its clinical applications.METHODS:Thenewnear-visionchartwhichcombined the Bailey-Lovie layout with a newly devised set of symmetry symbols w...AIM:To introduce a new near-vision chart for children aged 3-5 years old and its clinical applications.METHODS:Thenewnear-visionchartwhichcombined the Bailey-Lovie layout with a newly devised set of symmetry symbols was designed based on WeberFechner law.It consists of 15 rows of symmetry symbols,corresponding to a visual acuity range from 1.3 to 0.1logMAR.The optotypes were red against a white background and were specially shaped four basic geometric symbols:circle,square,triangle,and cross,which matched the preschool children’s cognitive level.A regular geometric progression of the optotype sizes and distribution was employed to arrange in 15 lines.The progression rate of the optotype size between two lines was 1.2589 and two smaller groups of optotypes ranging from 0.7 to-0.1 logMAR were included for repetitive testing.A near visual acuity was recorded in logMAR or decimal,and the testing distance was 25 cm.RESULTS:This new near-vision chart with pediatric acuity test optotypes which consists of 4 different symbols(triangle,square,cross,and circle)met the national and international eye chart design guidelines.When performing the near visual acuity assessment in preschoolers(3-5y old).It overcame an inability to recognize the letters of the alphabet and difficulties in designating the direction of black abstract symbols such as the tumbling’E’or Landolt’C’,which the subjects were prone to lose interest in.Near vision may be recorded in different notations:decimal acuity and logMAR.These two notations can be easily convertedeach other in the new near-vision chart.The measurements of this new chart not only showed a significant correlation and a good consistency with the Chinese national standard logarithmic near-vision chart(r=0.932,P【0.01),but also indicated good test-retest reliability(89%of retest scores were within 0.1 logMAR units of the initial test score)and a high response rate.CONCLUSION:The results of this study support the validity and reliability of near visual acuity measurements using the new near-vision chart in children aged 3-5y over a wide range of visual acuities,and the new eye chart was especially suitable for the detection of amblyopia risk factors and low vision examination in children(3-5y of age).It can be applied in routine clinical practice.展开更多
Tumor vaccine therapy offers significant advantages over conventional treatments,including reduced toxic side effects.However,it currently functions primarily as an adjuvant treatment modality in clinical oncology due...Tumor vaccine therapy offers significant advantages over conventional treatments,including reduced toxic side effects.However,it currently functions primarily as an adjuvant treatment modality in clinical oncology due to limitations in tumor antigen selection and delivery methods.Tumor vaccines often fail to elicit a sufficiently robust immune response against progressive tumors,thereby limiting their clinical efficacy.In this study,we developed a nanoparticle-based tumor vaccine,OVA@HA-PEI,utilizing ovalbumin(OVA)as the presenting antigen and hyaluronic acid(HA)and polyethyleneimine(PEI)as adjuvants and carriers.This formulation significantly enhanced the proliferation of immune cells and cytokines,such as CD3,CD8,interferon-,and tumor necrosis factor-,in vivo,effectively activating an immune response against B16–F10 tumors.In vivofluorescenceflow cytometry(IVFC)has already become an effective method for monitoring circulating tumor cells(CTCs)due to its direct,noninvasive,and long-term detection capabilities.Our study utilized a laboratory-constructed IVFC system to monitor the immune processes induced by the OVA@HA-PEI tumor vaccine and an anti-programmed death-1(PD-1)antibody.The results demonstrated that the combined treatment of OVA@HA-PEI and anti-PD-1 antibody significantly improved the survival time of mice compared to anti-PD-1 antibody treatment alone.Additionally,this combination therapy substantially reduced the number of CTCs in vivo,increased the clearance rate of CTCs by the immune system,and slowed tumor progression.Thesefindings greatly enhance the clinical application prospects of IVFC and tumor vaccines.展开更多
As one of the seaweed polysaccharide, agarose has received much attention because of its biocompatibility. However, its application in biomedical field was limited with its biological inertia. Modification with some f...As one of the seaweed polysaccharide, agarose has received much attention because of its biocompatibility. However, its application in biomedical field was limited with its biological inertia. Modification with some functional groups is needed to obtain agarose derivatives with biological activity and expand its applications. Consequently, agarose was sulfated with chlorosulfonic acid-pyridine with formamide as dispersing agent. The orthogonal test result showed that the optimal reaction condition was the reaction time being 4 h, the reaction temperature 65 ℃ and the ratio of chlorosulfonic acid to agarose 1-4（mL/g）. Two kinds of the insoluble agarose sulfate （below 37 ℃） were synthesized with degree of substitution （DS） being 0.17 and 0.43 respectively. Infrared spectroscopy （IR） and tac nuclear magnetic resonance （13C-NMR） spectroscopy results proved that C3_6 in agarose was sulfated. Their hydrophobic property and BSA adsorption capacity rose with increasing DS, while the adsorption of lib was reduced. The anti-clotting properties of agarose sulfate were significantly improved, and agarose sulfate could protect red blood cells from deformation after adsorption of BSA. These findings demonstrate that the cold-water insoluble agarose sulfate has a promise for applications as heoarin-like material in anticoagulation or endothelial re^enera＇Lion scaffold.展开更多
基金funding from the National Key Research and Development Program of China(2021YFF0502900)special fund for Research on the National Major Research Instruments of China(62027824)+2 种基金the National Natural Science Foundation of China(U24A20314)the Key Research and Development Program of Anhui Province in China(2022a05020028)the Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province.
文摘The ability to noninvasively manipulate and isolate specific cell populations in vivo is critical for advancing real-time diagnostics,precision medicine,and immunological research.Here,we present a novel and broadly applicable optical trapping system based on a custom-designed 2×3 optical tweezer array,which enables the real-time interception and manipulation of circulating leukocytes in live animals.By utilizing intrinsic velocity differences between leukocytes and red blood cells,the system achieves stable trapping of individual leukocytes in vessels 15-20μm in diameter and decelerates multiple cells in vessels greater than 20μm.Notably,it also enables the optical blockage of lymphatic vessels exceeding 50μm,a previously unreported capability.This label-free,noninvasive approach operates without repeated blood draws and is compatible with diverse vessel geometries and flow dynamics.The system offers a generalizable solution for in vivo cell extraction and analysis,paving the way for high-precision single-cell technologies in biomedical research and clinical translation.
文摘Gastrointestinal(GI)cancers remain a leading cause of cancer-related morbidity and mortality worldwide.Artificial intelligence(AI),particularly machine learning and deep learning(DL),has shown promise in enhancing cancer detection,diagnosis,and prognostication.A narrative review of literature published from January 2015 to march 2025 was conducted using PubMed,Web of Science,and Scopus.Search terms included"gastrointestinal cancer","artificial intelligence","machine learning","deep learning","radiomics","multimodal detection"and"predictive modeling".Studies were included if they focused on clinically relevant AI applications in GI oncology.AI algorithms for GI cancer detection have achieved high performance across imaging modalities,with endoscopic DL systems reporting accuracies of 85%-97%for polyp detection and segmentation.Radiomics-based models have predicted molecular biomarkers such as programmed cell death ligand 2 expression with area under the curves up to 0.92.Large language models applied to radiology reports demonstrated diagnostic accuracy comparable to junior radiologists(78.9%vs 80.0%),though without incremental value when combined with human interpretation.Multimodal AI approaches integrating imaging,pathology,and clinical data show emerging potential for precision oncology.AI in GI oncology has reached clinically relevant accuracy levels in multiple diagnostic tasks,with multimodal approaches and predictive biomarker modeling offering new opportunities for personalized care.However,broader validation,integration into clinical workflows,and attention to ethical,legal,and social implications remain critical for widespread adoption.
文摘Efficient strategies to promote microvascularization in vascular tissue engineering,a central priority in regenerative medicine,are still scarce;nano-and micro-sized aggregates and spheres or beads harboring primitive microvascular beds are promising methods in vascular tissue engineering.Capillaries are the smallest type and in numerous blood vessels,which are distributed densely in cardiovascular system.To mimic this microvascular network,specific cell components and proangiogenic factors are required.Herein,advanced biofabrication methods in microvascular engineering,including extrusion-based and droplet-based bioprinting,Kenzan,and biogripper approaches,are deliberated with emphasis on the newest works in prevascular nano-and micro-sized aggregates and microspheres/microbeads.
基金863 Program grant number: 2077AA09Z436+1 种基金Guangdong Province '211' Fund for Biomaterials and Tissue Engineering grantnumber: 50621030
文摘A PVA-GAG-COL composite scaffold is fabricated by polyvinyl alcohol （PVA）, glyeosaminoglycan （GAG） and collagen （COL）. Laser surface modification technology is used to make holes on the surface of the scaffolds. Inside and outside interconnection micro-porous structure is obtained. Bioeompatibility test of the scaffolds shows that PVA-GAG-COL scaffold can promote the adhesion and proliferation of the fibroblast. Also, fibroblast can grow normally on the scaffolds with pore diameter from 115 um to 255 um and pore distance from 500 um to 2000 um. PVA-GAG-COL scaffolds possess excellent cell biocompatibility. The porous structure is suitable for cell culture in tissue engineering.
基金supported by Korean Fund for Regenerative Medicine funded by Ministry of Science and ICT,and Ministry of Health and Welfare(22A0106L1,Republic of Korea)the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.2022M3C1A3081359).
文摘The brain exhibits complex physiology characterized by unique features such as a brain-specific extracellular matrix, compartmentalized structure (white and grey matter), and an aligned axonal network. These physiological characteristics underpin brain function and facilitate signal transduction similar to that in an electrical circuit. Therefore, investigating these features in vitro is crucial for understanding the interactions between neuronal signal transduction processes and the pathology of neurological diseases. Compared to neurons on patterned substrates, three-dimensional (3D) bioprinting-based neural models provide significant advantages in replicating axonal kinetics without physical limitations. This study proposes the development of a 3D bioprinted engineered neural network (BENN) model to replicate the physiological features of the brain, suggesting its application as a tool for studying neurodegenerative diseases. We employed 3D bioprinting to reconstruct the compartmentalized structure of the brain, and controlled the directionality of axonal growth by applying electrical stimuli to the printed neural structure for overcoming spatial constraints. The reconstructed axonal network demonstrated reliability as a neural analog, including the visualization of mature neuronal features and spontaneous calcium reactions. Furthermore, these brain-like neural network models have demonstrated usefulness for studying neurodegeneration by enabling the visualization of degenerative pathophysiology in alcohol-exposed neurons. The BENN facilitates the visualization of region-specific pathological markers in soma or axon populations, including amyloid-beta formation and axonal deformation. Overall, the BENN closely mimics brain physiology, offers insights into the dynamics of axonal networks, and can be applied to studying neurological diseases.
基金The 100th Anniversary Chulalongkorn University Fund for Doctoral Scholarshipthe National Budget to CULAC,Grant/Award Number:390252002+1 种基金the 90th Anniversary of Chulalongkorn University Fund,Grant/Award Number:GCUGR1125641042Dthe Thailand Science Research and Innovation Fund at Chulalongkorn University,Grant/Award Number:HEAF673100100。
文摘Background:Endoplasmic reticulum(ER)stress is an important factor in the development of numerous cardiovascular disorders;nevertheless,the association between ER stress and mitral regurgitation(MR)remains inadequately characterized.The molecular mechanism of pimobendan(PIMO)that contributes to the delay in congestive heart failure(CHF)in MR associated with apoptosis and fibrosis is still unclear.Our aim was to examine the impact of PIMO on ER stress,apoptosis,and fibrosis in a chronic MR rat model.Methods:MR was surgically induced in 10 Sprague–Dawley rats,with 5 serving as sham operation controls.At 8 weeks postsurgery,the MR animals were randomly allocated into two groups:MR and MR+PIMO groups.PIMO was administered twice daily through oral gavage for 4 weeks,whereas the sham and MR groups were administered similar quantities of drinking water.Echocardiography was conducted before the delivery of PIMO as a baseline measure and at the end of the study.At the end of the investigation,hearts were procured for histopathological and ER stress evaluations.Results:PIMO significantly maintained heart function and structural remodeling in the MR animals.PIMO significantly reduced MR-induced myocyte apoptosis(p=0.044)and fibrosis(p=0.002)by reducing the messenger RNA expression of genes associated with ER stress(GRP78[glucose-regulated protein 78],ATF4[activating transcription factor 4],and CHOP[C/ERP homologous protein])compared to the MR group(p<0.05,p<0.01,and p<0.001,respectively).Conclusion:PIMO demonstrated cardioprotective benefits on heart function,myocyte apoptosis,and fibrosis by regulating ER stress in an MR-induced CHF rat model.
基金supported by the National Key Re-search and Development Program of China(2021YFF0502900,2019YFC1604604)the grant of Peak Climbing Project of Foshan Hospital of Tra-ditional Chinese Medicine,Traditional Chinese Medicine Bureat of Guangdong Province Project(No.20213018)+2 种基金the Special Fund for Research on National Major Research Instruuments of China(Grant No.62027824)Scientific Research Fund of Education Department of Yunnan Province(2023Y0619)Biomedical Projects of Yun-nan Key Science and Technology Program(202302AA310046).
文摘Triple-negative bresst canær(TNBC)metastscis is particularly severe due to its aggressive nsture,leading to rapid disease progresion and significantly reduced survival rates.Rujifang(RJF),a traditional Chinese formula,has demonstrated potential anti-tumor effects and theability to inhibit TNBC metastasis.However,the efects af varying R.IF dors remain undear.This study utilized Laser-based in vino fow cytometry(IVFC)to monitor circulating tumor cells(CTCs)and evaluate the efficacy of R.IF at different doses.The results indicated that R.IF at the high dose inhibited both the number af CTC:and the formaton of metatatic foci more eflectively compared to the lower dose.TUNEL assays revealed that R.IF trentment promotes apoptosis of tumor cells,with a more pronounced effect observed at the higher dose.Immuno-fluorescence experiments demonstrated that administering a higher dose of R.IF suppreses theеxprescion of Kindlin-1 more effectively in the tumor microenvironment.Although higher doses showed enhanced efficacy,they might also lesd to an increase in side efects.These findings underscore the promise and challenges of using R.IF at high doses for anti-tumor therspy.They highlight the criticnl importance of optimizing the dose of R.JP in the treatment of TNBC and provide valuable insights for its dinical application.
基金Supported by the National Natural Science Foundation of China:81173206
文摘Background:Observing interstitial fluid(ISF)is very difficult because interstitial structure collapses and ISF disappears after tissue fixation.Additionally,ISF is colorless,and interstitial flow is weak in vivo.In order to view the interstitial flow,special dye and animal model was chosen to explore the movement characteristic of interstitial flow and related structure.Methods:The Gephyrocharax Melanocheir(GM)fish,a special animal with translucent body,were placed into 0.03 g/L tricain solution for anesthesia.20-25μL Alcian blue(AB)solution which can stain acid mucopolysaccharide immobilized by the collagen net in connective tissue was injected into each fish at a single point with a rate of 2 μL/min via a micro-injection pump.The process of infusion and the movement of the AB in fish were record by a digital camera.The(fresh)frozen sections of AB tracks tissue were performed to observe the morphological feature.Results:Several blue tracks were observed which were formed by longitudinal directional movements of AB solution.For back lateral track,the velocity and length of the movements were significantly fast and longer on the direction toward head than that toward tail(P<0.01).For lateral middle track,the result was opposite,namely toward tail(P<0.01).This phenomenon indicated an inherent ISF flow according to Darcy's law.Morphological study showed these tracks were just in septa composed by connective tissue.The stained blue septa formed various shapes as interstitial space for ISF flow and connected with one another like a net.The finding can help us to understand the essence of meridian in traditional Chinese medicine(TCM).Conclusions:The dynamic asymmetry of the AB tracks revealed interstitial flow in the GM fish body.It implied an inherent interstitial flow along particular pathway formed by septa which may be a key to understand the nature and value of meridians and collaterals in health care.
基金supported by grants from the National Key Research and Development Program of China (2020YFC2002800 to J.L. and 2018YFC1105102 to J.L.)the National Natural Science Foundation of China (91949127, 92168204 to J.L.)the Fundamental Research Funds for the Central Universities (22120210586)
文摘Prostaglandin E2(PGE2), a major cyclooxygenase-2(COX-2) product, is highly secreted by the osteoblast lineage in the subchondral bone tissue of osteoarthritis(OA) patients. However, NSAIDs, including COX-2 inhibitors, have severe side effects during OA treatment. Therefore, the identification of novel drug targets of PGE2 signaling in OA progression is urgently needed. Osteoclasts play a critical role in subchondral bone homeostasis and OA-related pain. However, the mechanisms by which PGE2 regulates osteoclast function and subsequently subchondral bone homeostasis are largely unknown. Here, we show that PGE2 acts via EP4 receptors on osteoclasts during the progression of OA and OA-related pain. Our data show that while PGE2 mediates migration and osteoclastogenesis via its EP2 and EP4 receptors, tissue-specific knockout of only the EP4 receptor in osteoclasts(EP4 Lys M) reduced disease progression and osteophyte formation in a murine model of OA. Furthermore, OA-related pain was alleviated in the EP4 Lys M mice, with reduced Netrin-1 secretion and CGRP-positive sensory innervation of the subchondral bone. The expression of plateletderived growth factor-BB(PDGF-BB) was also lower in the EP4 Lys Mmice, which resulted in reduced type H blood vessel formation in subchondral bone. Importantly, we identified a novel potent EP4 antagonist, HL-43, which showed in vitro and in vivo effects consistent with those observed in the EP4 Lys Mmice. Finally, we showed that the Gαs/PI3 K/AKT/MAPK signaling pathway is downstream of EP4 activation via PGE2 in osteoclasts. Together, our data demonstrate that PGE2/EP4 signaling in osteoclasts mediates angiogenesis and sensory neuron innervation in subchondral bone, promoting OA progression and pain, and that inhibition of EP4 with HL-43 has therapeutic potential in OA.
文摘AIM:To Introduce a new specialized visual acuity chart for amblyopic children aged 3-5 years old and its clinical applications.METHODS:The new visual acuity chart and notations were designed based on Weber-Fechner law.The optotypes were red against a white background and were specially shaped four basic geometric symbols:circle,square,triangle,and cross.A regular geometric progression of the optotype sizes and distribution was employed to arrange in 14 lines.The progression rate of the optotype size between two lines was 1.2589 and the testing distance was 3m.Visual acuity score could be recorded as logMAR notation or decimal notation.Agestratified diagnostic criteria for amblyopia established by consensus statement on diagnosis of amblyopia(2011)among members of the Strabismus and Pediatric Ophthalmology Group,Ophthalmology Society,Chinese Medical Association(SPOGOSCMA)were illustrated in the new visual acuity chart.RESULTS:When assessing visual acuity in children aged 3-5 years old,this new visual acuity chart that consists of four symmetrical shapes(triangle,square,cross,and circle)overcame an inability to recognize the letters of the alphabet and difficulties in designating the direction of black abstract symbols such as the tumbling’E’or Landolt’C’,which the subjects were prone to lose interest in.The visual acuity score may be recorded indifferent notations:decimal acuity and logMAR.These two notations can be easily converted each other in the new eye chart.The measurements of this new chart not only showed a significant correlation and a good consistency with the international standard logarithmic visual acuity chart(r=0.932,P【0.01),but also indicated a high test-retest reliability(89%of retest scores were within 0.1 logMAR units of the initial test score).CONCLUSION:The results of this study support the validity and reliability of distance visual acuity measurements using the new eye chart in children aged3 to 5 years over a wide range of visual acuities,and the new eye chart is great for early detection of amblyopia.It can be applied in various clinical settings.
基金supported by a"KRCF National Agenda Project",by an Asan Life Science Institute Grant(12-241)from the Asan Medical Center,Seoul,Korea
文摘Transplantation of neural stem cells has been reported as a possible approach for replacing impaired dopaminergic neurons. In this study, we tested the efficacy of early-stage human dental papilla-derived stem cells and human brain-derived neural stem cells in rat models of 6-hydroxydopamine-induced Parkinson＇s disease. Rats received a unilateral injection of 6-hydroxydopamine into right medial forebrain bundle, followed 3 weeks later by injections of PBS, early-stage human dental papilla-derived stem cells, or human brain-derived neural stem cells into the ipsilateral striatum. All of the rats in the human dental papilla-derived stem cell group died from tumor formation at around 2 weeks following cell transplantation. Postmortem examinations revealed homogeneous malignant tumors in the striatum of the human dental papilla-derived stem cell group. Stepping tests revealed that human brain-derived neural stem cell transplantation did not improve motor dysfunction. In apomorphine-induced rotation tests, neither the human brain-derived neural stem cell group nor the control groups （PBS injection） demonstrated significant changes. Glucose metabolism in the lesioned side of striatum was reduced by human brain-derived neural stem cell transplantation. [18F]-FP-CIT PET scans in the striatum did not demonstrate a significant increase in the human brain-derived neural stem cell group. Tyrosine hydroxylase （dopaminergic neuronal marker） staining and G protein-activated inward rectifier potassium channel 2 （A9 dopaminergic neuronal marker） were positive in the lesioned side of striatum in the human brain-derived neural stem cell group. The use of early-stage human dental papilla-derived stern cells confirmed its tendency to form tumors. Human brain-derived neural stem cells could be partially differentiated into dopaminergic neurons, but they did not secrete dopamine.
基金supported by grants from National Key Technology R&D Program(Grant No.:2011BAI12B06)the Fundamental Research Funds for the Central Universities(Grant No.:2012FZA5023).
文摘The subtle color distinction is the important function of electronic endoscope imaging diagnosis.However,after image acquisition,transmission and display,color distortions of intracorporeal organs or tissues occur inevitably,which are adverse to analyze image features accurately or to diagnose early pathological changes.A real-time color correction algorithm based on fourneighborhood and polynomial regression in YUV color space is proposed.Based on polynomial regression the color correction matrix is calculated in YUV color space according to the dierences between standard values of color checker and measured values of that imaged by the endoscope.As the correction is only executed on U and V components in YUV color space,the defect that the color of corrected images in RGB color space will change along with luminance can be avoided,and then the stability of image color is improved.Owing to four-neighborhood processing,the signal-to-noise ratio of corrected images is enhanced and the processing speed of correction algorithm is accelerated.The average color dierence is reduced from 0.3944 to 0.2850 by application of the proposed algorithm in high-denition electronic endoscope.A total of 17 frames per second can be achieved at the resolution of 1280800,and the color characteristics of the image after processing match that of human visual system.
基金Supported by the Research Council of Lithuania,No.S-MIP-19-8and the Research,Development and Innovation Fund of Kaunas University of Technology,No.MTEPI-L-17003 and MTEPI-L-16012.
文摘BACKGROUND Degree of portal hypertension(PH)is the most important prognostic factor for the decompensation of liver cirrhosis and death,therefore adequate care for patients with liver cirrhosis requires timely detection and evaluation of the presence of clinically significant PH(CSPH)and severe PH(SPH).As the most accurate method for the assessment of PH is an invasive direct measurement of hepatic venous pressure gradient(HVPG),the search for non-invasive methods to diagnose these conditions is actively ongoing.AIM To evaluate the feasibility of parameters of endogenously induced displacements and strain of liver to assess degree of PH.METHODS Of 36 patients with liver cirrhosis and measured HVPG were included in the casecontrol study.Endogenous motion of the liver was characterized by derived parameters of region average tissue displacement signal(dantero,dretro,dRMS)and results of endogenous tissue strain imaging using specific radiofrequency signal processing algorithm.Average endogenous strainμand standard deviationσof strain were assessed in the regions of interest(ROI)(1 cm×1 cm and 2 cm×2 cm in size)and different frequency subbands of endogenous motion(0-10 Hz and 10-20 Hz).RESULTS Four parameters showed statistically significant(P<0.05)correlation with HVPG measurement.The strongest correlation was obtained for the standard deviation of strain(estimated at 0-10 Hz and 2 cm×2 cm ROI size).Three parameters showed statistically significant differences between patient groups with CSPH,but only dretro showed significant results in SPH analysis.According to ROC analysis area under the curve(AUC)of theσROI[0…10Hz,2 cm×2 cm]parameter reached 0.71(P=0.036)for the diagnosis of CSPH;with a cut-off value of 1.28μm/cm providing 73%sensitivity and 70%specificity.AUC for the diagnosis of CSPH forμROI[0…10Hz,1 cm×1 cm]was 0.78(P=0.0024);with a cut-off value of 3.92μm/cm providing 73%sensitivity and 80%specificity.Dretro parameter had an AUC of 0.86(P=0.0001)for the diagnosis of CSPH and 0.84(P=0.0001)for the diagnosis of SPH.A cut-off value of-132.34μm yielded 100%sensitivity for both conditions,whereas specificity was 80%and 72%for CSPH and SPH respectively.CONCLUSION The parameters of endogenously induced displacements and strain of the liver correlated with HVPG and might be used for non-invasive diagnosis of PH.
基金supported by the National Natural Science Foundation of China(No.30900730)the grants from the Natural Science Foundation of Shangdong Province(No.Q2007D01)
文摘Objective：To generate recombinant adenovirus that could simultaneously express ornithine decarboxylase（ODC） and S-adenosylmethionine decarboxylase（AdoMetDC） antisenses specifically in prostate cancer cells,and evaluate its inhibitory effect on prostate cancer in vivo.Methods：Fragments of ODC and AdoMetDC genes were generated by PCR,cloned into the pPGL-PSES,and then recombined with pAdEasy-1 vectors in AdEasy-1 cells.Ad-PSES-ODC-AdoMetDCas virus was produced in HEK293 cells.Following transfection with Ad-PSES-ODC-AdoMetDCas,the levels of ODC or AdoMetDC were determined by RT-PCR and western blot assays.The effect of Ad-PSES-ODC-AdoMetDCas treatment on tumor formation and growth was evaluated in xenograft models of prostate cancers in vivo.Results：The plasmid pAdEasy-PSES-ODC-AdoMetDCas was successfully constructed and the recombinant Ad-PSES-ODC-AdoMetDCas adenovirus was produced.Transfection with Ad-PSES-ODC-AdoMetDCas adenovirus significantly inhibited the expression of ODC and AdoMetDC genes specifically in prostate DU145 cells,but not H1299,HT29 and HepG2 cancer cells,and disrupted the ability of DU145 cells to form solid prostate cancer in vivo.Intratumoral treatment with Ad-PSES-ODC-AdoMetDCas adenovirus significantly inhibited the growth of engrafted prostate tumors in vivo.Conclusion：The recombinant Ad-PSES-ODC-AdoMetDCas adenovirus specifically reduces the expression of both ODC and AdoMetDC genes in prostate cells and may be used for treatment of prostate cancers at the clinic.
基金Supported by Major Science and Technology Innovation Projects of Shandong Province,No.2018CXGC1310Natural Foundation of Shandong Province,No.ZR2019MH134+2 种基金Project of Jinan"20 Universities",No.2019GXRC040Shandong Institute of Advanced Technology,Chinese Academy of Sciences,No.YJZX003Collaborative Innovation Center Project of quality Control of traditional Chinese Medicine and Construction of whole Industry chain in Colleges and Universities of Shandong Province,No.CYLXTCX2020-04.
文摘BACKGROUND Lumbar disc herniation(LDH)has emerged as one of the most common causes of low back pain.The routine treatment approach involves chemonucleolysis therapy,discectomy by percutaneous endoscopy,and percutaneous laser disc decompression.Unfortunately,all of these methods carry inherent risk of causing harm to the patient and,as such,there is an unmet but urgent need for an effective and safe noninvasive treatment for LDH.The purpose of this report is to describe a non-invasive method for re-absorption of LDH.CASE SUMMARY A 34-year-old woman was admitted with a complaint of waist pain that she reported as having become acutely aggravated over the past 3 d and accompanied by discomfort in the right lower limb.Her self-reported medical history included persistent postpartum low back pain from 7 years prior.Physical exam showed positivity for neck flexion test(Lindner sign)and supine abdomen test;the straight leg-raising test showed right 60(+)and left 80(-).Findings from standard imaging(magnetic resonance)and collective physical examinations indicated a L5/S1 herniated lumbar disc.Treatment consisted of three-dimensional(balanced regulating)spinal manipulation and acupuncture,upon which the LDH resolved by retraction.CONCLUSION Following L5/S1 herniated lumbar disc diagnosis,three-dimensional(balanced regulating)spinal manipulation combined with acupuncture therapy is an effective treatment.
文摘AIM:To introduce a new near-vision chart for children aged 3-5 years old and its clinical applications.METHODS:Thenewnear-visionchartwhichcombined the Bailey-Lovie layout with a newly devised set of symmetry symbols was designed based on WeberFechner law.It consists of 15 rows of symmetry symbols,corresponding to a visual acuity range from 1.3 to 0.1logMAR.The optotypes were red against a white background and were specially shaped four basic geometric symbols:circle,square,triangle,and cross,which matched the preschool children’s cognitive level.A regular geometric progression of the optotype sizes and distribution was employed to arrange in 15 lines.The progression rate of the optotype size between two lines was 1.2589 and two smaller groups of optotypes ranging from 0.7 to-0.1 logMAR were included for repetitive testing.A near visual acuity was recorded in logMAR or decimal,and the testing distance was 25 cm.RESULTS:This new near-vision chart with pediatric acuity test optotypes which consists of 4 different symbols(triangle,square,cross,and circle)met the national and international eye chart design guidelines.When performing the near visual acuity assessment in preschoolers(3-5y old).It overcame an inability to recognize the letters of the alphabet and difficulties in designating the direction of black abstract symbols such as the tumbling’E’or Landolt’C’,which the subjects were prone to lose interest in.Near vision may be recorded in different notations:decimal acuity and logMAR.These two notations can be easily convertedeach other in the new near-vision chart.The measurements of this new chart not only showed a significant correlation and a good consistency with the Chinese national standard logarithmic near-vision chart(r=0.932,P【0.01),but also indicated good test-retest reliability(89%of retest scores were within 0.1 logMAR units of the initial test score)and a high response rate.CONCLUSION:The results of this study support the validity and reliability of near visual acuity measurements using the new near-vision chart in children aged 3-5y over a wide range of visual acuities,and the new eye chart was especially suitable for the detection of amblyopia risk factors and low vision examination in children(3-5y of age).It can be applied in routine clinical practice.
基金supported by the National Key Research and Development Program of China,Grant Number:2021YFF0502900,2019YFC1604604National Natural Science Foundation of China,Grant Number:62075013,62027824.
文摘Tumor vaccine therapy offers significant advantages over conventional treatments,including reduced toxic side effects.However,it currently functions primarily as an adjuvant treatment modality in clinical oncology due to limitations in tumor antigen selection and delivery methods.Tumor vaccines often fail to elicit a sufficiently robust immune response against progressive tumors,thereby limiting their clinical efficacy.In this study,we developed a nanoparticle-based tumor vaccine,OVA@HA-PEI,utilizing ovalbumin(OVA)as the presenting antigen and hyaluronic acid(HA)and polyethyleneimine(PEI)as adjuvants and carriers.This formulation significantly enhanced the proliferation of immune cells and cytokines,such as CD3,CD8,interferon-,and tumor necrosis factor-,in vivo,effectively activating an immune response against B16–F10 tumors.In vivofluorescenceflow cytometry(IVFC)has already become an effective method for monitoring circulating tumor cells(CTCs)due to its direct,noninvasive,and long-term detection capabilities.Our study utilized a laboratory-constructed IVFC system to monitor the immune processes induced by the OVA@HA-PEI tumor vaccine and an anti-programmed death-1(PD-1)antibody.The results demonstrated that the combined treatment of OVA@HA-PEI and anti-PD-1 antibody significantly improved the survival time of mice compared to anti-PD-1 antibody treatment alone.Additionally,this combination therapy substantially reduced the number of CTCs in vivo,increased the clearance rate of CTCs by the immune system,and slowed tumor progression.Thesefindings greatly enhance the clinical application prospects of IVFC and tumor vaccines.
基金863 High-tech Plan (No. 2007AA09Z436)NNSFC(No. 31070853)+2 种基金Guangdong Technology Plan (No. 2010B031100015)Guangzhou Technology Plan (No. 2010-EJ0041)"12-5" National Tech Program
文摘As one of the seaweed polysaccharide, agarose has received much attention because of its biocompatibility. However, its application in biomedical field was limited with its biological inertia. Modification with some functional groups is needed to obtain agarose derivatives with biological activity and expand its applications. Consequently, agarose was sulfated with chlorosulfonic acid-pyridine with formamide as dispersing agent. The orthogonal test result showed that the optimal reaction condition was the reaction time being 4 h, the reaction temperature 65 ℃ and the ratio of chlorosulfonic acid to agarose 1-4（mL/g）. Two kinds of the insoluble agarose sulfate （below 37 ℃） were synthesized with degree of substitution （DS） being 0.17 and 0.43 respectively. Infrared spectroscopy （IR） and tac nuclear magnetic resonance （13C-NMR） spectroscopy results proved that C3_6 in agarose was sulfated. Their hydrophobic property and BSA adsorption capacity rose with increasing DS, while the adsorption of lib was reduced. The anti-clotting properties of agarose sulfate were significantly improved, and agarose sulfate could protect red blood cells from deformation after adsorption of BSA. These findings demonstrate that the cold-water insoluble agarose sulfate has a promise for applications as heoarin-like material in anticoagulation or endothelial re^enera＇Lion scaffold.
