Tapuy is an indigenous wine produced in the Philippines.Rice wine fermentation produces a by-product of rice leftover and microbial biomass,collectively called lees,which usually is discarded as a waste product.Howeve...Tapuy is an indigenous wine produced in the Philippines.Rice wine fermentation produces a by-product of rice leftover and microbial biomass,collectively called lees,which usually is discarded as a waste product.However,studies have shown the potential value of lees as a healthy food source.We wanted to determine the nutritional profile of tapuy wine and its lees and their abilities to extend lifespan in Caenorhabditis elegans.Tapuy lees(7.65 g/100 g dry weight)was 18.5-fold greater in protein content compared to tapuy wine.Its sugar content(27.66 g/100 g dry weight)is similar to the tapuy wine’s sugar content(23.465 g/100 g dry weight).Through LC-MS/MS,18.75%and 12.39%of spectral peaks in tapuy wine and lees were matched,respectively,to specific compounds,and several of them are associated with beneficial health effects.Furthermore,the phenolic content in tapuy lees(19,475.526 Gallic Acid Equivalent)is 6.5-fold greater compared to tapuy wine.Surprisingly,the DPPH and FRAP antioxidant assays show that tapuy lees had a lesser antioxidant ability compared to tapuy wine.However,C.elegans fed with supplementation of tapuy lees extract showed a 16.24%increase in mean lifespan,which is higher compared to the 6.10%increase in mean lifespan when supplemented with tapuy wine.Taking all these findings together,our study revealed that tapuy lees has a greater nutritional value than tapuy wine,and this underutilized and wastefully discarded product can be repurposed as a potential functional food.展开更多
Epithelial–mesenchymal transition(EMT)is a biological process that involves the transformation of epithelial cells into cells with a mesenchymal phenotype,enhancing their migratory and invasive capabilities.EMT is cr...Epithelial–mesenchymal transition(EMT)is a biological process that involves the transformation of epithelial cells into cells with a mesenchymal phenotype,enhancing their migratory and invasive capabilities.EMT is crucial in embryonic development,tissue healing,and wound repair,and aids in forming diverse cell types and structures.However,aberrant EMT is involved in pathogenic processes,including fibrosis,cancer development,and progression.Recent studies show that EMT contributes to resistance to cancer treatment,including epidermal growth factor receptor-tyrosine kinase inhibitor(EGFR-TKI)therapy in non-small cell lung cancer(NSCLC).This review discusses the intricate relationship between EMT and acquired chemoresistance to EGFR-TKIs.It details evidence on how EGFR-TKIs might induce EMT and how EMT may cause EGFR-TKI resistance.Understanding these pathways is crucial for developing effective prognostic and therapeutic strategies to predict and combat acquired chemoresistance in NSCLC,advancing the field toward more targeted and personalized treatment approaches.展开更多
基金funded by the Philippine Institute of Traditional and Alternative Health Care(PITAHC).
文摘Tapuy is an indigenous wine produced in the Philippines.Rice wine fermentation produces a by-product of rice leftover and microbial biomass,collectively called lees,which usually is discarded as a waste product.However,studies have shown the potential value of lees as a healthy food source.We wanted to determine the nutritional profile of tapuy wine and its lees and their abilities to extend lifespan in Caenorhabditis elegans.Tapuy lees(7.65 g/100 g dry weight)was 18.5-fold greater in protein content compared to tapuy wine.Its sugar content(27.66 g/100 g dry weight)is similar to the tapuy wine’s sugar content(23.465 g/100 g dry weight).Through LC-MS/MS,18.75%and 12.39%of spectral peaks in tapuy wine and lees were matched,respectively,to specific compounds,and several of them are associated with beneficial health effects.Furthermore,the phenolic content in tapuy lees(19,475.526 Gallic Acid Equivalent)is 6.5-fold greater compared to tapuy wine.Surprisingly,the DPPH and FRAP antioxidant assays show that tapuy lees had a lesser antioxidant ability compared to tapuy wine.However,C.elegans fed with supplementation of tapuy lees extract showed a 16.24%increase in mean lifespan,which is higher compared to the 6.10%increase in mean lifespan when supplemented with tapuy wine.Taking all these findings together,our study revealed that tapuy lees has a greater nutritional value than tapuy wine,and this underutilized and wastefully discarded product can be repurposed as a potential functional food.
文摘Epithelial–mesenchymal transition(EMT)is a biological process that involves the transformation of epithelial cells into cells with a mesenchymal phenotype,enhancing their migratory and invasive capabilities.EMT is crucial in embryonic development,tissue healing,and wound repair,and aids in forming diverse cell types and structures.However,aberrant EMT is involved in pathogenic processes,including fibrosis,cancer development,and progression.Recent studies show that EMT contributes to resistance to cancer treatment,including epidermal growth factor receptor-tyrosine kinase inhibitor(EGFR-TKI)therapy in non-small cell lung cancer(NSCLC).This review discusses the intricate relationship between EMT and acquired chemoresistance to EGFR-TKIs.It details evidence on how EGFR-TKIs might induce EMT and how EMT may cause EGFR-TKI resistance.Understanding these pathways is crucial for developing effective prognostic and therapeutic strategies to predict and combat acquired chemoresistance in NSCLC,advancing the field toward more targeted and personalized treatment approaches.
