Droplet microfluidics enable high-throughput screening,sequencing,and formulation of biological and chemical systems at the microscale.Such devices are generally fabricated in a soft polymer such as polydimethylsiloxa...Droplet microfluidics enable high-throughput screening,sequencing,and formulation of biological and chemical systems at the microscale.Such devices are generally fabricated in a soft polymer such as polydimethylsiloxane(PDMS).However,developing design masks for PDMS devices can be a slow and expensive process,requiring an internal cleanroom facility or using an external vendor.Here,we present the first complete droplet-based component library using low-cost rapid prototyping and electrode integration.This fabrication method for droplet microfluidic devices costs less than$12 per device and a full design-build-test cycle can be completed within a day.Discrete microfluidic components for droplet generation,re-injection,picoinjection,anchoring,fluorescence sensing,and sorting were built and characterized.These devices are biocompatible,low-cost,and high-throughput.To show its ability to perform multistep workflows,these components were used to assemble droplet"pixel"arrays,where droplets were generated,sensed,sorted,and anchored onto a grid to produce images.展开更多
Synthetic biology is the process of forward engineering living systems.These systems can be used to produce biobased materials,agriculture,medicine,and energy.One approach to designing these systems is to employ techn...Synthetic biology is the process of forward engineering living systems.These systems can be used to produce biobased materials,agriculture,medicine,and energy.One approach to designing these systems is to employ techniques from the design of embedded electronics.These techniques include abstraction,standards,modularity,automated design,and formal semantic models of computation.Together,these elements form the foundation of“biodesign automation,”where software,robotics,and microfluidic devices combine to create exciting biological systems of the future.This paper describes a“hardware,software,wetware”codesign vision where software tools can be made to act as“genetic compilers”that transform high-level specifications into engineered“genetic circuits”(wetware).This is followed by a process where automation equipment,welldefined experimental workflows,and microfluidic devices are explicitly designed to house,execute,and test these circuits(hardware).These systems can be used as either massively parallel experimental platforms or distributed bioremediation and biosensing devices.Next,scheduling and control algorithms(software)manage these systems’actual execution and data analysis tasks.A distinguishing feature of this approach is how all three of these aspects(hardware,software,and wetware)may be derived from the same basic specification in parallel and generated to fulfill specific cost,performance,and structural requirements.展开更多
基金supported by NSF Semiconductor Synthetic Biology for Information Storage and Retrieval(Award#2027045)。
文摘Droplet microfluidics enable high-throughput screening,sequencing,and formulation of biological and chemical systems at the microscale.Such devices are generally fabricated in a soft polymer such as polydimethylsiloxane(PDMS).However,developing design masks for PDMS devices can be a slow and expensive process,requiring an internal cleanroom facility or using an external vendor.Here,we present the first complete droplet-based component library using low-cost rapid prototyping and electrode integration.This fabrication method for droplet microfluidic devices costs less than$12 per device and a full design-build-test cycle can be completed within a day.Discrete microfluidic components for droplet generation,re-injection,picoinjection,anchoring,fluorescence sensing,and sorting were built and characterized.These devices are biocompatible,low-cost,and high-throughput.To show its ability to perform multistep workflows,these components were used to assemble droplet"pixel"arrays,where droplets were generated,sensed,sorted,and anchored onto a grid to produce images.
基金the National Natural Science Foundation of China Grant No.2211040“Collaborative Research:Model-guided design of bacterial interspecies interactions and trans-organismic communication in living intercellular circuits”(S.M.D.O.and D.D.)NSF Grant No.2027045“SemiSynBio-II:Hybrid Bio-Electronic Microfluidic Memory Arrays for Large Scale Testing and Remote Deployment”(S.M.D.O and D.D).
文摘Synthetic biology is the process of forward engineering living systems.These systems can be used to produce biobased materials,agriculture,medicine,and energy.One approach to designing these systems is to employ techniques from the design of embedded electronics.These techniques include abstraction,standards,modularity,automated design,and formal semantic models of computation.Together,these elements form the foundation of“biodesign automation,”where software,robotics,and microfluidic devices combine to create exciting biological systems of the future.This paper describes a“hardware,software,wetware”codesign vision where software tools can be made to act as“genetic compilers”that transform high-level specifications into engineered“genetic circuits”(wetware).This is followed by a process where automation equipment,welldefined experimental workflows,and microfluidic devices are explicitly designed to house,execute,and test these circuits(hardware).These systems can be used as either massively parallel experimental platforms or distributed bioremediation and biosensing devices.Next,scheduling and control algorithms(software)manage these systems’actual execution and data analysis tasks.A distinguishing feature of this approach is how all three of these aspects(hardware,software,and wetware)may be derived from the same basic specification in parallel and generated to fulfill specific cost,performance,and structural requirements.
