Background:Pistacia integerrima,a cornerstone of traditional medicine,is renowned for its therapeutic applications against various health conditions,including cancer and hepatitis.This study investigates the pharmacol...Background:Pistacia integerrima,a cornerstone of traditional medicine,is renowned for its therapeutic applications against various health conditions,including cancer and hepatitis.This study investigates the pharmacological potential of bioactive compounds derived from Pistacia integerrima in inhibiting 5-lipoxygenase(5-LOX),a key enzyme implicated in inflammation and cancer progression.The current study aimed to evaluate the lipoxygenase inhibitory activity of bioactive compounds from Pistacia integerrima and assess their potential for therapeutic development in the context of inflammation and cancer treatment.Methods:Three major compounds-spinacetin(1),patuletin(2),and pistagremic acid(3)-were isolated from Pistacia integerrima and analyzed for their lipoxygenase inhibitory activity.Biochemical assays and molecular docking studies were performed to assess their effectiveness in inhibiting 5-LOX.Results:All three compounds demonstrated significant inhibition of lipoxygenase activity.Spinacetin(1)and patuletin(2)exhibited the most potent inhibitory effects,with IC_(50)values of 40.34μM and 45.04μM,respectively.Molecular docking studies revealed that patuletin(2)had the highest binding affinity(−7.717 kcal/mol)against 5-LOX,followed by spinacetin(1)with a binding affinity of−6.074 kcal/mol.In-depth in silico analysis highlighted the drug-likeness of spinacetin(1)and its favorable toxicological profile,suggesting its suitability for therapeutic development.Conclusion:The study demonstrates that compounds from Pistacia integerrima,particularly spinacetin and patuletin,have significant lipoxygenase inhibitory activity,with spinacetin showing promise as a lead candidate for lipoxygenase-targeted therapies.The findings reinforce the therapeutic relevance of Pistacia integerrima and suggest that its bioactive compounds may serve as safer,plant-based alternatives to conventional anti-inflammatory and anticancer treatments.展开更多
Cucumber mosaic virus(CMV)is among the most widespread plant viruses,infecting over a thousand plant species,including Catharanthus roseus,a medicinal plant valued for producing the anticancer alkaloids vincristine an...Cucumber mosaic virus(CMV)is among the most widespread plant viruses,infecting over a thousand plant species,including Catharanthus roseus,a medicinal plant valued for producing the anticancer alkaloids vincristine and vinblastine.Despite its economic significance,genomic information on CMV infecting C.roseus in India has been lacking.In this study,we employed small RNA deep sequencing integrated with advanced bioinformatics to generate the first complete genome of CMV infecting C.roseus in India,followed by validation through RT-PCR and Sanger sequencing.The reconstructed tripartite CMV genome encodes replication,silencing suppressor,movement,and coat proteins,consistent with known genomic organization.Sequence identity and phylogenetic analyses revealed a close relationship with Iranian tobacco isolates,placing the Indian isolate(CR8-JH)within subgroup IB.Recombination analysis indicated inter-fragmental recombination events,while mutational profiling highlighted natural variation,particularly in genes associated with host interaction.Conserved UTR motifs were identified,suggesting functional roles in viral replication and genome stability.By applying next-generation sequencing for accurate viral detection and characterization,this study enhances diagnostic capacity and contributes to technology-driven,sustainable plant disease management.展开更多
文摘Background:Pistacia integerrima,a cornerstone of traditional medicine,is renowned for its therapeutic applications against various health conditions,including cancer and hepatitis.This study investigates the pharmacological potential of bioactive compounds derived from Pistacia integerrima in inhibiting 5-lipoxygenase(5-LOX),a key enzyme implicated in inflammation and cancer progression.The current study aimed to evaluate the lipoxygenase inhibitory activity of bioactive compounds from Pistacia integerrima and assess their potential for therapeutic development in the context of inflammation and cancer treatment.Methods:Three major compounds-spinacetin(1),patuletin(2),and pistagremic acid(3)-were isolated from Pistacia integerrima and analyzed for their lipoxygenase inhibitory activity.Biochemical assays and molecular docking studies were performed to assess their effectiveness in inhibiting 5-LOX.Results:All three compounds demonstrated significant inhibition of lipoxygenase activity.Spinacetin(1)and patuletin(2)exhibited the most potent inhibitory effects,with IC_(50)values of 40.34μM and 45.04μM,respectively.Molecular docking studies revealed that patuletin(2)had the highest binding affinity(−7.717 kcal/mol)against 5-LOX,followed by spinacetin(1)with a binding affinity of−6.074 kcal/mol.In-depth in silico analysis highlighted the drug-likeness of spinacetin(1)and its favorable toxicological profile,suggesting its suitability for therapeutic development.Conclusion:The study demonstrates that compounds from Pistacia integerrima,particularly spinacetin and patuletin,have significant lipoxygenase inhibitory activity,with spinacetin showing promise as a lead candidate for lipoxygenase-targeted therapies.The findings reinforce the therapeutic relevance of Pistacia integerrima and suggest that its bioactive compounds may serve as safer,plant-based alternatives to conventional anti-inflammatory and anticancer treatments.
基金Deanship of Research and Graduate Studies,University of Tabuk,Kingdom of Saudi Arabia(0176-1442-S)Research,Development,and Innovation Authority(RDIA)-Kingdom of Saudi Arabia(13445-Tabuk-2023-UT-R-3-1-SE).
文摘Cucumber mosaic virus(CMV)is among the most widespread plant viruses,infecting over a thousand plant species,including Catharanthus roseus,a medicinal plant valued for producing the anticancer alkaloids vincristine and vinblastine.Despite its economic significance,genomic information on CMV infecting C.roseus in India has been lacking.In this study,we employed small RNA deep sequencing integrated with advanced bioinformatics to generate the first complete genome of CMV infecting C.roseus in India,followed by validation through RT-PCR and Sanger sequencing.The reconstructed tripartite CMV genome encodes replication,silencing suppressor,movement,and coat proteins,consistent with known genomic organization.Sequence identity and phylogenetic analyses revealed a close relationship with Iranian tobacco isolates,placing the Indian isolate(CR8-JH)within subgroup IB.Recombination analysis indicated inter-fragmental recombination events,while mutational profiling highlighted natural variation,particularly in genes associated with host interaction.Conserved UTR motifs were identified,suggesting functional roles in viral replication and genome stability.By applying next-generation sequencing for accurate viral detection and characterization,this study enhances diagnostic capacity and contributes to technology-driven,sustainable plant disease management.
