Objective: To test the antioxidant, antimicrobial and a-glucosidase inhibitory activities of the roots extracts from Hertia cheirifolia(H. cheirifolia) L.Methods: Total phenolics and total flavonoids content of the di...Objective: To test the antioxidant, antimicrobial and a-glucosidase inhibitory activities of the roots extracts from Hertia cheirifolia(H. cheirifolia) L.Methods: Total phenolics and total flavonoids content of the different extracts were determined by colorimetric methods and reverse phase high-performance liquid chromatography(RP-HPLC) was performed to identify various chemical components.The different extracts were evaluated for antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl(DPPH), 2,2-azino-bis-3-ethylenebenzothiozoline-6-sulfonic acid(ABTS·^+) and β-carotene bleaching tests and α-glucosidase inhibitory properties. The antimicrobial activity was carried out in vitro by the broth dilution method.Results: Trans-cinnamic acid, rutin hydrate, naringin and quercetin were the main compounds of the ethyl acetate extract from H. cheirifolia L. This extract has significant scavenging activity to decrease free radicals especially for DPPH and ABTS radicals. As well as, the ethyl acetate extract exhibited an antimicrobial property against bacterial strains. Bacillus licheniformis and Salmonella enterica were the most sensitive strains with minimum inhibitory concentration values of 0.156 mg/mL.Conclusion: The ethyl acetate extract was found to be selectively antioxidant and antimicrobial.展开更多
Background: Chagas disease is still a public health problem because of the remaining high prevalence, the expansion of the disease into developed countries and the re-emergences by oral transmission outbreaks. Chagas ...Background: Chagas disease is still a public health problem because of the remaining high prevalence, the expansion of the disease into developed countries and the re-emergences by oral transmission outbreaks. Chagas cardiomyopathy evolves as a consequence of an autonomic un-balance where the parasympathetic tone is undermined. Objective: To determine the functionality and expression of muscarinic cholinergic receptors in acute Chagas disease. Methodology: 62 male, 3-week-old Sprague Dawley rats were assayed;32 were infected with Trypanosoma cruzi trypo-mastigotes and 30 were healthy controls. Electrocardiographic studies were conducted in the absence or presence of direct muscarinic (oxotremorine and McN-A-343) or indirect agonists (phenylephrine) or antagonist (pirenzepine). Muscarinic M1 and M2 receptor expression was determined by radioligand [3H]-QNB binding assay and immunoblot. Results: Chagasic acute myocarditis was sustained by electrocardiographic signs and histopathological findings. Bradycardia induced by oxotremorine was significantly higher in healthy rats (HR) and the differences were enhanced by CsCl. In the absence of the agonist, CsCl induced a greater bradycardia in chagasic rats (ChR). In HR McN-A-343 induced tachycardia, however it induces bradycardia in the presence of a acetylcholinesterase inhibitor (neostigmine);no effects were observed in ChR. Pirenzepine induced a higher tachycardia in HR. Phenylephrine in the presence of pirenzepine induced a similar bradycardia in both groups, but recovery was faster in ChR. Muscarinic M1 and M2 receptor density was higher in HR. Conclusion: Muscarinic receptor expression and functionality are decreased in the acute Chagas disease that could impact the evolution and prognosis of the disease.展开更多
Objective: To examine the potential antimicrobial activity of Euphorbia paralias L. (Euphorbiaeae) leaves and stems extracts. Methods: The antimicrobial activity was tested against six microbial strains:Escherichia co...Objective: To examine the potential antimicrobial activity of Euphorbia paralias L. (Euphorbiaeae) leaves and stems extracts. Methods: The antimicrobial activity was tested against six microbial strains:Escherichia coli ATCC 8739, Bacillus subtilis ATCC 6633, Salmonella enterica CIP 8039, Staphy-lococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027 and Candida albicans ATCC 90028 by two different methods, the disk method and the dilution method. Results: Our results showed the important antimicrobial activity of the chloroform extract of the stems towards the majority of the strains by using both methods. Bacillus subtilis was the most sensitive strain (MIC=MBC=15μg/mL). Conclusion: Thus, some extracts of Euphorbia paralias can be used in the treatment of infectious diseases caused by microbes.展开更多
Levan is a fructose homopolymer obtained from both plants and microorganisms.Microbial levan is typically produced in a variety of molecular weights(MW).Previous studies have shown that different MW of levan exhibit d...Levan is a fructose homopolymer obtained from both plants and microorganisms.Microbial levan is typically produced in a variety of molecular weights(MW).Previous studies have shown that different MW of levan exhibit different biological activities.Though levan was reported for its immunomodulatory effect by increasing nitric oxide(NO)production in RAW264.7 macrophage cells,whether its MW can affect this activity remains unknown.In this study,the effect of different levan MW from Bacillus subtilis on its immunoregulatory properties was investigated.B.subtilis was cultivated using various cultivation parameters to obtain levan of different MW.Three weight average MW of levan,>8×10^(5) Da(M1),1.5×10^(4) Da(M2)and 3.7×10^(3) Da(M3),were selected to test for their effect on modulating NO levels in RAW264.7 cells.Our results showed that M2 increased NO production in a dose-dependent manner,and more effectively than that of M1 and M3.Consistent with these findings,M2 significantly stimulated inducible nitric oxide synthase(iNOS)and cyclooxygenase-2(COX2)protein expression,while less pronounced effects were observed in the cells treated with M1 and M3.Therefore,our results demonstrate that various MW of levan have varying effects on its immunoregulatory activities in RAW264.7 cells.Furthermore,our findings suggest that the size of levan should be taken into account when employing levan as one of the active ingredients in functional foods and immunomodulatory products.展开更多
Objective:To investigate the antibacterial activity of essential oils from Hertia cheirifolia L.flowers,vegetative part(leaves+stems)and roots against a panel of bacterial strains.Methods:The essential oils from Herti...Objective:To investigate the antibacterial activity of essential oils from Hertia cheirifolia L.flowers,vegetative part(leaves+stems)and roots against a panel of bacterial strains.Methods:The essential oils from Hertia cheirifolia L.flowers,vegetative part(leaves+stems)and roots were analyzed by gas chromatography and gas chromatography-mass spectrometry.The antibacterial activity of essential oils was evaluated in vitro by the broth dilution method.Results:The results showed that the essential oil of flowers exhibited a strong antibacterial activity against Staphylococcus aureus with minimal inhibitory concentration of 0.078 mg/mL.Conclusions:The essential oils of Hertia cheirifolia can be a good source of antibacterial compounds.They can be used for pharmaceutical treatments.展开更多
基金funded by the Ministry of High Education,Scientific Research and Technology of Tunisia(Grant No.11/TM06)
文摘Objective: To test the antioxidant, antimicrobial and a-glucosidase inhibitory activities of the roots extracts from Hertia cheirifolia(H. cheirifolia) L.Methods: Total phenolics and total flavonoids content of the different extracts were determined by colorimetric methods and reverse phase high-performance liquid chromatography(RP-HPLC) was performed to identify various chemical components.The different extracts were evaluated for antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl(DPPH), 2,2-azino-bis-3-ethylenebenzothiozoline-6-sulfonic acid(ABTS·^+) and β-carotene bleaching tests and α-glucosidase inhibitory properties. The antimicrobial activity was carried out in vitro by the broth dilution method.Results: Trans-cinnamic acid, rutin hydrate, naringin and quercetin were the main compounds of the ethyl acetate extract from H. cheirifolia L. This extract has significant scavenging activity to decrease free radicals especially for DPPH and ABTS radicals. As well as, the ethyl acetate extract exhibited an antimicrobial property against bacterial strains. Bacillus licheniformis and Salmonella enterica were the most sensitive strains with minimum inhibitory concentration values of 0.156 mg/mL.Conclusion: The ethyl acetate extract was found to be selectively antioxidant and antimicrobial.
文摘Background: Chagas disease is still a public health problem because of the remaining high prevalence, the expansion of the disease into developed countries and the re-emergences by oral transmission outbreaks. Chagas cardiomyopathy evolves as a consequence of an autonomic un-balance where the parasympathetic tone is undermined. Objective: To determine the functionality and expression of muscarinic cholinergic receptors in acute Chagas disease. Methodology: 62 male, 3-week-old Sprague Dawley rats were assayed;32 were infected with Trypanosoma cruzi trypo-mastigotes and 30 were healthy controls. Electrocardiographic studies were conducted in the absence or presence of direct muscarinic (oxotremorine and McN-A-343) or indirect agonists (phenylephrine) or antagonist (pirenzepine). Muscarinic M1 and M2 receptor expression was determined by radioligand [3H]-QNB binding assay and immunoblot. Results: Chagasic acute myocarditis was sustained by electrocardiographic signs and histopathological findings. Bradycardia induced by oxotremorine was significantly higher in healthy rats (HR) and the differences were enhanced by CsCl. In the absence of the agonist, CsCl induced a greater bradycardia in chagasic rats (ChR). In HR McN-A-343 induced tachycardia, however it induces bradycardia in the presence of a acetylcholinesterase inhibitor (neostigmine);no effects were observed in ChR. Pirenzepine induced a higher tachycardia in HR. Phenylephrine in the presence of pirenzepine induced a similar bradycardia in both groups, but recovery was faster in ChR. Muscarinic M1 and M2 receptor density was higher in HR. Conclusion: Muscarinic receptor expression and functionality are decreased in the acute Chagas disease that could impact the evolution and prognosis of the disease.
基金Supported by the Ministry of High Education and Scientific Research,MHSSR of Tunisia(Grant No.11/TM06)
文摘Objective: To examine the potential antimicrobial activity of Euphorbia paralias L. (Euphorbiaeae) leaves and stems extracts. Methods: The antimicrobial activity was tested against six microbial strains:Escherichia coli ATCC 8739, Bacillus subtilis ATCC 6633, Salmonella enterica CIP 8039, Staphy-lococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027 and Candida albicans ATCC 90028 by two different methods, the disk method and the dilution method. Results: Our results showed the important antimicrobial activity of the chloroform extract of the stems towards the majority of the strains by using both methods. Bacillus subtilis was the most sensitive strain (MIC=MBC=15μg/mL). Conclusion: Thus, some extracts of Euphorbia paralias can be used in the treatment of infectious diseases caused by microbes.
基金financially supported by the Research and Development Fund,Burapha University(Grant no.12/2562&10/2565)the Science Innovation Facility,Faculty of Science,Burapha University(SIF-IN-60300003)+2 种基金the Center of Excellence for Innovation in Chemistry(PERCH-CIC)the Commission on Higher Education,Ministry of Education,Thailandthe Scientific Instruments Center,School of Science,King Mongkut’s Institute of Technology Ladkrabang.
文摘Levan is a fructose homopolymer obtained from both plants and microorganisms.Microbial levan is typically produced in a variety of molecular weights(MW).Previous studies have shown that different MW of levan exhibit different biological activities.Though levan was reported for its immunomodulatory effect by increasing nitric oxide(NO)production in RAW264.7 macrophage cells,whether its MW can affect this activity remains unknown.In this study,the effect of different levan MW from Bacillus subtilis on its immunoregulatory properties was investigated.B.subtilis was cultivated using various cultivation parameters to obtain levan of different MW.Three weight average MW of levan,>8×10^(5) Da(M1),1.5×10^(4) Da(M2)and 3.7×10^(3) Da(M3),were selected to test for their effect on modulating NO levels in RAW264.7 cells.Our results showed that M2 increased NO production in a dose-dependent manner,and more effectively than that of M1 and M3.Consistent with these findings,M2 significantly stimulated inducible nitric oxide synthase(iNOS)and cyclooxygenase-2(COX2)protein expression,while less pronounced effects were observed in the cells treated with M1 and M3.Therefore,our results demonstrate that various MW of levan have varying effects on its immunoregulatory activities in RAW264.7 cells.Furthermore,our findings suggest that the size of levan should be taken into account when employing levan as one of the active ingredients in functional foods and immunomodulatory products.
基金Supported by the Ministry of High Education and Scientific Research,Tunisia(Grant No.11/TM06).
文摘Objective:To investigate the antibacterial activity of essential oils from Hertia cheirifolia L.flowers,vegetative part(leaves+stems)and roots against a panel of bacterial strains.Methods:The essential oils from Hertia cheirifolia L.flowers,vegetative part(leaves+stems)and roots were analyzed by gas chromatography and gas chromatography-mass spectrometry.The antibacterial activity of essential oils was evaluated in vitro by the broth dilution method.Results:The results showed that the essential oil of flowers exhibited a strong antibacterial activity against Staphylococcus aureus with minimal inhibitory concentration of 0.078 mg/mL.Conclusions:The essential oils of Hertia cheirifolia can be a good source of antibacterial compounds.They can be used for pharmaceutical treatments.
