The coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)affected health,economies,and lifestyles,but little is known about its impact on children.We aimed to...The coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)affected health,economies,and lifestyles,but little is known about its impact on children.We aimed to investigate changes in 24-hour(h)movement behaviours of pre-school children before and during COVID-19,and factors that influenced these.Children aged 3-6 years were recruited in Beijing in 2019,and their movement behaviours over 24h assessed by questionnaire,as part of the International Study of Movement Behaviors in the Early Years(SUNRISE)study.We conducted the survey again during COVID-19,and compared the children’s movement behaviours before and during COVID-19 and associated factors.Overall,196 parents completed the survey at both time points.The percentage of children meeting movement guidelines decreased during the pandemic(p<0.01).Total physical activity decreased less among children getting good quality sleep or with more adults in the household(p<0.01).Children who were not cared for primarily by their mother had a greater decrease in moderate to vigorous intensity physical activity(p=0.02),but the decrease was smaller among children whose parents used the internet to support their physical activity and/or screen time(p<0.05).Children who used electronic screen devices in the 2h before bedtime or whose parents reported body temperature in webchats had a greater increase in sedentary screen time(p<0.05).Children spending more time outdoors showed a smaller decrease in sleep(p<0.01).Overall,children’s movement behaviours changed significantly during the pandemic.展开更多
Objective:To explore the effect and mechanism of SEMA3C/3D mutations on axonal growth of neurons and cell migration in Hirschsprung′s disease(HSCR)patients.Methods:HEK293 Tcells were transfected with wild-type and mu...Objective:To explore the effect and mechanism of SEMA3C/3D mutations on axonal growth of neurons and cell migration in Hirschsprung′s disease(HSCR)patients.Methods:HEK293 Tcells were transfected with wild-type and mutant SEMA3C/3D plasmids.The supernatants that contained SEMA3C/3D-AP fusion proteins were collected and added into the Neuro-2acells.The changes in the cell morphology were observed by immunofluorescence staining.The expression and phosphorylation levels of cofilin,ERM and CRMP2 were determined by western blotting.The cell migration rate was measured by transwell assay.Results:Compared with wild-type SEMA3D,SEMA3D-P615T mutant suppressed cofilin phosphorylation in Neuro-2a cells(P <0.05).The neural cells treated by five mutant SEMA3C/3D-AP fusion proteins presented different levels of axon atrophy,growth cone collapse,and sometimes,loss of normal structure.SEMA3C-S329 G,SEMA3C-V337M and SEMA3D-P615T mutants cells exhibited a significantly reduced migration rate as compared with wild-type SEMA3C/3D treated cells(P <0.01).Conclusion:SEMA3C and SEMA3 D mutations in HSCR patients could lead to the inhibition of Neuro-2a cells′migration and axonal growth.The mechanism of SEMA3 DP615T mutant might be related to down-regulation of the expression of p-cofilin,which consequently lead to cytoskeleton structure collapse and migrating ability decrease.Our study might provide important clues for the pathogenesis of HSCR.展开更多
Objective Circular RNAs(circRNAs)participate in several important pathological processes and have been used in the diagnosis and treatment of various diseases.This study aimed to investigate the role of circRNAs in ne...Objective Circular RNAs(circRNAs)participate in several important pathological processes and have been used in the diagnosis and treatment of various diseases.This study aimed to investigate the role of circRNAs in neural tube defects(NTDs).Method We characterized circRNA-associated competitive endogenous RNA(ceRNA)networks in brain tissue of low folate-induced NTDs mouse at embryonic day 13.5 by high-throughput sequencing.The expression levels of Circzfp644,miR-20-5p and Gas7 were detected by RT-PCR.Gas7 and Circzfp644functions were determined by miRNA-mimics and inhibitors in mouse teratocarcinoma cells(F9 cells),and luciferase gene reporter assay was assessed in the F9 cells.In addition,the expression levels of Circzfp644,miR-20-5p and Gas7 were determined by Nanostring in human NTDs tissues.Results We detected 57 circRNA transcripts,16 miRNAs,and 148 mRNAs that were significantly dysregulated in NTDs brain tissues compared with their expression levels in control(normal)tissues.Circzfp644 shared miRNA response elements with the growth arrest specific 7(Gas7)gene and competitively bound with miR-20-5p to increase the expression of Gas7.Downregulation of Circzfp644and Gas7 and upregulation of miR-20-5p were found in human NTD tissue.Conclusion This study provides new perspectives on the role of circRNAs in nervous system development and the pathogenesis of NTDs.展开更多
基金upported by Public service development and reform pilot project of Beijing Medical Research Institute(BMR2021-3).
文摘The coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)affected health,economies,and lifestyles,but little is known about its impact on children.We aimed to investigate changes in 24-hour(h)movement behaviours of pre-school children before and during COVID-19,and factors that influenced these.Children aged 3-6 years were recruited in Beijing in 2019,and their movement behaviours over 24h assessed by questionnaire,as part of the International Study of Movement Behaviors in the Early Years(SUNRISE)study.We conducted the survey again during COVID-19,and compared the children’s movement behaviours before and during COVID-19 and associated factors.Overall,196 parents completed the survey at both time points.The percentage of children meeting movement guidelines decreased during the pandemic(p<0.01).Total physical activity decreased less among children getting good quality sleep or with more adults in the household(p<0.01).Children who were not cared for primarily by their mother had a greater decrease in moderate to vigorous intensity physical activity(p=0.02),but the decrease was smaller among children whose parents used the internet to support their physical activity and/or screen time(p<0.05).Children who used electronic screen devices in the 2h before bedtime or whose parents reported body temperature in webchats had a greater increase in sedentary screen time(p<0.05).Children spending more time outdoors showed a smaller decrease in sleep(p<0.01).Overall,children’s movement behaviours changed significantly during the pandemic.
基金supported by the National Natural Science Foundation of China(No.81300266)Beijing Natural Science Foundation(No.7142029)+1 种基金Beijing Excellent Scientist Fund(No.2013D003034000007)Beijing Novo Program(No.Z151100000315091)
文摘Objective:To explore the effect and mechanism of SEMA3C/3D mutations on axonal growth of neurons and cell migration in Hirschsprung′s disease(HSCR)patients.Methods:HEK293 Tcells were transfected with wild-type and mutant SEMA3C/3D plasmids.The supernatants that contained SEMA3C/3D-AP fusion proteins were collected and added into the Neuro-2acells.The changes in the cell morphology were observed by immunofluorescence staining.The expression and phosphorylation levels of cofilin,ERM and CRMP2 were determined by western blotting.The cell migration rate was measured by transwell assay.Results:Compared with wild-type SEMA3D,SEMA3D-P615T mutant suppressed cofilin phosphorylation in Neuro-2a cells(P <0.05).The neural cells treated by five mutant SEMA3C/3D-AP fusion proteins presented different levels of axon atrophy,growth cone collapse,and sometimes,loss of normal structure.SEMA3C-S329 G,SEMA3C-V337M and SEMA3D-P615T mutants cells exhibited a significantly reduced migration rate as compared with wild-type SEMA3C/3D treated cells(P <0.01).Conclusion:SEMA3C and SEMA3 D mutations in HSCR patients could lead to the inhibition of Neuro-2a cells′migration and axonal growth.The mechanism of SEMA3 DP615T mutant might be related to down-regulation of the expression of p-cofilin,which consequently lead to cytoskeleton structure collapse and migrating ability decrease.Our study might provide important clues for the pathogenesis of HSCR.
基金supported by the National Natural Sciences [82071690,81971390]Research Foundation of Capital Institute of Pediatrics [FX-2020-05,CXYJ-2-21-09]+1 种基金Public service development and reform pilot project of Beijing Medical Research Institute [BMR2021-1]Beijing Hospitals Authority Clinical technology innovation program [XLMX 202110]。
文摘Objective Circular RNAs(circRNAs)participate in several important pathological processes and have been used in the diagnosis and treatment of various diseases.This study aimed to investigate the role of circRNAs in neural tube defects(NTDs).Method We characterized circRNA-associated competitive endogenous RNA(ceRNA)networks in brain tissue of low folate-induced NTDs mouse at embryonic day 13.5 by high-throughput sequencing.The expression levels of Circzfp644,miR-20-5p and Gas7 were detected by RT-PCR.Gas7 and Circzfp644functions were determined by miRNA-mimics and inhibitors in mouse teratocarcinoma cells(F9 cells),and luciferase gene reporter assay was assessed in the F9 cells.In addition,the expression levels of Circzfp644,miR-20-5p and Gas7 were determined by Nanostring in human NTDs tissues.Results We detected 57 circRNA transcripts,16 miRNAs,and 148 mRNAs that were significantly dysregulated in NTDs brain tissues compared with their expression levels in control(normal)tissues.Circzfp644 shared miRNA response elements with the growth arrest specific 7(Gas7)gene and competitively bound with miR-20-5p to increase the expression of Gas7.Downregulation of Circzfp644and Gas7 and upregulation of miR-20-5p were found in human NTD tissue.Conclusion This study provides new perspectives on the role of circRNAs in nervous system development and the pathogenesis of NTDs.
