The functions of photoreceptors have been extensively investigated over the last two decades.However,whether those photoreceptors have any functions in the absence of light remained unclear.Recently,Prof.Hongtao Liu’...The functions of photoreceptors have been extensively investigated over the last two decades.However,whether those photoreceptors have any functions in the absence of light remained unclear.Recently,Prof.Hongtao Liu’s team at Shenzhen University reports that Arabidopsis blue-light receptor cryptochrome 2(CRY2)is physiologically functional in darkness(Zeng et al.,2024),solving this long-standing question.These researchers discovered that monomeric CRY2 interacted with transcription factors FL1/3 to suppress FL1/3-dependent cell division in roots in darkness,avoiding excessive energy consumption due to the overgrowth of roots in the absence of light.They also discovered that blue light inhibited the CRY2-FL1/3 interaction to promote cell division,root growth,and photomorphogenesis under light conditions.展开更多
Photoreceptor cryptochromes (CRYs) mediate blue-light regulation of plant growth and development. It has been reported that Arabidopsis CRY1and CRY2 function by physically interacting with at least 84 proteins, includ...Photoreceptor cryptochromes (CRYs) mediate blue-light regulation of plant growth and development. It has been reported that Arabidopsis CRY1and CRY2 function by physically interacting with at least 84 proteins, including transcription factors or co-factors, chromatin regulators, splicing factors, messenger RNA methyltransferases, DNA repair proteins, E3 ubiquitin ligases, protein kinases and so on. Of these 84 proteins, 47 have been reported to exhibit altered binding affinity to CRYs in response to blue light, and 41 have been shown to exhibit condensation to CRY photobodies. The blue light-regulated composition or condensation of CRY complexes results in changes of gene expression and developmental programs. In this mini-review, we analyzed recent studies of the photoregulatory mechanisms of Arabidopsis CRY complexes and proposed the dual mechanisms of action, including the “Lock-and-Key” and the “Liquid-Liquid Phase Separation (LLPS)” mechanisms. The dual CRY action mechanisms explain, at least partially, the structural diversity of CRY-interacting proteins and the functional diversity of the CRY photoreceptors.展开更多
基金supported by the National Natural Science Foundation of China(32330009,32000155).
文摘The functions of photoreceptors have been extensively investigated over the last two decades.However,whether those photoreceptors have any functions in the absence of light remained unclear.Recently,Prof.Hongtao Liu’s team at Shenzhen University reports that Arabidopsis blue-light receptor cryptochrome 2(CRY2)is physiologically functional in darkness(Zeng et al.,2024),solving this long-standing question.These researchers discovered that monomeric CRY2 interacted with transcription factors FL1/3 to suppress FL1/3-dependent cell division in roots in darkness,avoiding excessive energy consumption due to the overgrowth of roots in the absence of light.They also discovered that blue light inhibited the CRY2-FL1/3 interaction to promote cell division,root growth,and photomorphogenesis under light conditions.
基金supported by the National Natural Science Foundation of China(32330009 and 32000155)China Postdoctoral Science Foundation(2020M670520,2021T140705).
文摘Photoreceptor cryptochromes (CRYs) mediate blue-light regulation of plant growth and development. It has been reported that Arabidopsis CRY1and CRY2 function by physically interacting with at least 84 proteins, including transcription factors or co-factors, chromatin regulators, splicing factors, messenger RNA methyltransferases, DNA repair proteins, E3 ubiquitin ligases, protein kinases and so on. Of these 84 proteins, 47 have been reported to exhibit altered binding affinity to CRYs in response to blue light, and 41 have been shown to exhibit condensation to CRY photobodies. The blue light-regulated composition or condensation of CRY complexes results in changes of gene expression and developmental programs. In this mini-review, we analyzed recent studies of the photoregulatory mechanisms of Arabidopsis CRY complexes and proposed the dual mechanisms of action, including the “Lock-and-Key” and the “Liquid-Liquid Phase Separation (LLPS)” mechanisms. The dual CRY action mechanisms explain, at least partially, the structural diversity of CRY-interacting proteins and the functional diversity of the CRY photoreceptors.
