Iris domestica,a perennial herb of the Iridaceae family,is widely recognized for its rich isoflavone content and broad therapeutic properties.To elucidate the biosynthetic pathway of these medicinally significant comp...Iris domestica,a perennial herb of the Iridaceae family,is widely recognized for its rich isoflavone content and broad therapeutic properties.To elucidate the biosynthetic pathway of these medicinally significant compounds,we constructed a haplotype-resolved genome assembly of this species.Transcriptomic and metabolomic analyses revealed tissue-specific accumulation of isoflavone,particularly in rhizomes and roots.Functional characterization identified two candidate isoflavone synthase genes,among which IdIFS was confirmed to promote the biosynthesis of key compounds tectorigenin and irisflorentin.The high-quality genome assembly presented here provides a foundational resource for further research into the evolution,secondary metabolite,and environmental adaptation of I.domestica.展开更多
Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100...Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100 COPD subjects from our hospital from January 2020 to December 2023 were included and randomly divided into a healthy control group and an experimental group(50 cases each).The healthy control group underwent slow vital capacity measurement using a spirometer,while the experimental group underwent high-resolution thin-slice CT scans and serum Vanin-1 and SPP1 concentration measurements.Pulmonary function parameters,symptom burden,biomarker concentrations,and imaging characteristics were compared between the two groups.Results:The FEV1/FVC ratio in the experimental group(58.3±7.2)was lower than that in the healthy control group(92.1±4.8);the total CAT score(22.4±3.5)was higher than that in the healthy control group(3.1±1.2);both Vanin-1(18.7±2.3μg/L)and SPP1(25.6±4.1μg/L)levels were higher than those in the healthy control group;LAA%-950(38.7±6.2%)and WA%(68.5±5.3%)were significantly higher than those in the healthy control group(all p<0.001).Conclusion:Chest CT combined with serum Vanin-1 and SPP1 can accurately quantify the pathological progression of COPD,providing a dual basis for clinical staging and individualized intervention.展开更多
Antibiotics are widespread in aquatic environments due to their extensive use in human healthcare and ani-mal husbandry.However,research on the occurrence and bioaccumulation of antibiotics in aquatic organisms within...Antibiotics are widespread in aquatic environments due to their extensive use in human healthcare and ani-mal husbandry.However,research on the occurrence and bioaccumulation of antibiotics in aquatic organisms within shallow wetland lakes remains limited.This study investigated the occurrence and bioaccumulation of ten commonly used antibiotics in the Baiyang Lake,northern China’s largest shallow wetland lake.The results indicated that sulfonamides and fluoroquinolones were the predominant antibiotics in surface water,whereas fluoroquinolones and macrolides were more prevalent in sediment.Fluoroquinolones demonstrated significant potential for bioaccumulation in targeted aquatic organisms,including both animals and plants(Carassius au-ratus and Phragmites australis).The bioaccumulation of antibiotics in Carassius auratus was correlated with their solubility,whereas in Phragmites australis,this was associated with their octanol-water partition coefficients and molecular weights.Ecological risk assessment indicated that most antibiotics posed minimal to low risk levels.However,four antibiotics were exceptions:clarithromycin(12.5%)and sulfamethoxazole(6.25%)presented a high risk in surface water samples,while norfloxacin(25.0%)and ciprofloxacin(25.0%)posed a high risk in sediment samples.Norfloxacin,ciprofloxacin,and roxithromycin were identified as key indicator antibiotics for enhancing the local monitoring and control of antibiotic contamination based on four criteria:(1)high con-centrations,(2)frequent detection,(3)capacity for bioaccumulation,and(4)ecological risk levels.This study contributes to a deeper understanding of the status of antibiotic contamination,bioaccumulation characteristics,and ecological risk in Baiyang Lake,thereby supporting efforts to monitor and regulate antibiotic pollution.展开更多
The rate capability and cycling stability of sodium metal batteries taking FeS_(2) or sulfur as cathode are limited due to their low reaction kinetics and severe shuttle effect.Herein,we rationally design a novel sing...The rate capability and cycling stability of sodium metal batteries taking FeS_(2) or sulfur as cathode are limited due to their low reaction kinetics and severe shuttle effect.Herein,we rationally design a novel single-atom-dispersed S_(2)-FeNC/FeS_(2) nanocluster heterojunction embedded in carbon spheres(SFNC/FeS_(2)) for the electrode material of sodium metal batteries.Interestingly,during the discharging process,the Na^(+) is inserted into FeS_(2) to generate Na_(2)S,as well as the unique electrochemical reaction between S_(2)-FeNC and Na^(+) to form Na_(2)S.Meanwhile,the FeNC can adsorb Na_(2)S and catalyze the conversion from Na_(2)S and Fe to FeS_(2) or from Na_(2)S and FeNC to S_(2)-FeNC for suppressing the shuttle effect and promoting the distinct hybrid reversible electrochemical behavior,which improves performance tremendously.Notably,the SFNC/FeS_(2) electrode delivers a specific capacity of 338.7 mAh g^(-1) after superlong 2000 cycles at a current density of 5.0 A g^(-1) and achieves a high energy density of 430.1 Wh Kg^(-1) at a current density of 0.05 A g^(-1).This work presents a novel approach to studying sodium metal batteries with hybrid behavior for excellent high energy density and cycling stability.展开更多
IR64 is an elite Xian/indica variety developed by International Rice Research Institute(IRRl)in 1985,which has been the most widely grown variety and core breeding parent in South/Southeast Asia(Mackill and Khush,2018...IR64 is an elite Xian/indica variety developed by International Rice Research Institute(IRRl)in 1985,which has been the most widely grown variety and core breeding parent in South/Southeast Asia(Mackill and Khush,2018).IR64 has been utilized to develop stress-tolerant(such as drought-adapted and submergenceresistant)near-isogenic lines,underscoring its great potential in agricultural genomics(Tanaka et al.,2020).展开更多
Fungi play crucial roles in nutrient acquisition,plant growth promotion,and the enhancement of resistance to both abiotic and biotic stresses.However,studies on the fungal communities associated with peas (Pisum sativ...Fungi play crucial roles in nutrient acquisition,plant growth promotion,and the enhancement of resistance to both abiotic and biotic stresses.However,studies on the fungal communities associated with peas (Pisum sativum L.) remain limited.In this study,we systematically investigated the ecological effects of host niches (soil,root,stem,leaf,and pod) and genotypes on the diversity and composition of fungal communities in peas using a multi-level approach that encompassed pattern recognition (β-diversity decomposition),mechanism validation (neutral community model testing),and dynamic tracking methods (migration pathway source-tracking).The results revealed that the dominant fungal phyla across niches and genotypes were Ascomycota,Basidiomycota,and Mortierellomycota,and the community structures of the soil–plant continuum were primarily determined by the pea niches rather than genotypes.β-diversity decomposition was largely attributed to species replacement rather than richness differences,indicating strong niche specificity and microbial replacement across microhabitats.Neutral model analysis revealed that stochastic processes influenced genotypeassociated communities,while deterministic processes played a dominant role in niche-based community assembly.Source-tracking analysis identified niche-to-niche fungal migration,with Erysiphe,Fusarium,Cephaliophora,Ascobolus,Alternaria,and Aspergillus as the key genera.Migration rates from exogenous to endogenous niches were low (1.3–61.5%),whereas those within exogenous (64.4–83.7%) or endogenous (73.9–96.4%) compartments were much higher,suggesting that the pea epidermis acts as a selective barrier that filters and enriches microbial communities prior to internal colonization.This study provides comprehensive insights into the mechanisms of host filtering,enrichment and microbial sourcing,which increases our understanding of the assembly rules of the pea-associated fungal microbiome.展开更多
Non-structural carbohydrates(NSCs)are crucial for tree growth and survival under climatic stress,yet their spatial dynamics across broad climate gradients remain unclear.Pines(Pinus spp.),one of the most widely distri...Non-structural carbohydrates(NSCs)are crucial for tree growth and survival under climatic stress,yet their spatial dynamics across broad climate gradients remain unclear.Pines(Pinus spp.),one of the most widely distributed tree genera worldwide,provide an ideal system for investigating large-scale spatial patterns of NSC within a single genus along extensive climatic gradients.Here,we compiled a global NSC database for pines across 74 sites,and assessed the spatial variation in total NSC,starch(St),and soluble sugars(SS)concentrations in stem sapwood,the primary reserve tissue,along site-specific mean annual temperature(MAT)and precipitation(MAP).Our results show that MAP exerted a stronger influence(R^(2)=20%–47%)on the spatial variation in total NSC and its components than did MAT(R^(2)=6%–16%).Tota concentrations declined nonlinearly with increasing MAP,with the rate of decline slowing beyond approximately 800 mm.While MAT had weaker effects on total NSC concentrations,both MAT and MAP jointly regulated NSC partitioning:Higher MAT and MAP were associated with reduced St concentrations but elevated SS concentrations and SS:St ratios.These findings suggest that pine species in cold and arid environments prioritize storing NSC as St,whereas in relatively warm and humid environments,NSC are preferentially mobilized into SS to support immediate metabolic and growth demands.展开更多
Bemisia tabaci is a complex of cryptic agro-economically important pest species characterized by diverse clades,substantial genetic diversity along with strong phylogeographic associations.However,a comprehensive phyl...Bemisia tabaci is a complex of cryptic agro-economically important pest species characterized by diverse clades,substantial genetic diversity along with strong phylogeographic associations.However,a comprehensive phylogenomic analysis across the entire complex has been lacking,we thus conducted phylogenomic analyses and explored biogeographic patterns using 680 single-copy nuclear genes(SCNs)obtained from whole-genome sequencing data of 58 globally sourced B.tabaci specimens.We constructed both concatenation and coalescent trees using 680 SCNs,which produced highly supported bootstrap values and nearly identical topologies for all major clades.When comparing these concatenation trees with those constructed using mitochondrial cytochrome oxidase I(mtCOI)and mitochondrial genome,we found conflicting phylogenetic relationships,with the later trees recovering fewer major clades.In a separate comparison between concatenation and coalescent trees,particularly those generated using IQ-TREE,they were found to delineate population relationships more effectively than RaxML.In contrast,coalescent phylogenies were proficient in elucidating geographical dispersal patterns and the reorganization of biological species.Furthermore,we provided a strict consensus tree that clearly defines relationships within most clades,laying a solid foundation for future research on the evolution and taxonomy of B.tabaci.Ancestral range estimates suggested that the ancestral region of the complex is likely situated in equatorial Africa,the Middle East,and Mediterranean regions.Subsequently,the expansion occurred into part of the Palearctic and further into the Nearctic,Neotropical,Indomalayan,and Australasian regions.These findings challenge both previous classifications and origin hypotheses,offering a notably more comprehensive understanding of the global distribution,evolutionary history,diversification,and biogeography of B.tabaci.展开更多
Immunotherapy for cardiovascular diseases(CVDs)holds great promise for precision management by modulating localized immune-inflammatory responses.The interplay between focal cardiovascular pathology and panvascular di...Immunotherapy for cardiovascular diseases(CVDs)holds great promise for precision management by modulating localized immune-inflammatory responses.The interplay between focal cardiovascular pathology and panvascular disease,necessitates highly integrated therapeutic strategies.Nano-technology-based theranostic platforms address this challenge by enabling both regulation and real-time imaging of immune cell activity within cardiovascular lesions.These functional nanotherapy systems not only halt disease progression at pathological sites but also reduce secondary cardiovascular events driven by shared inflammatory mechanisms.Additionally,nanoplatform-based dynamic visualization of immune cell responses facilitates adaptive,personalized interventions.This review introduces the role of immune cells in CVDs.It summarizes recent advances in nanomaterial-based immunomodulation strategies,including mechanisms of immune regulation,enhanced imaging,and therapeutic applications in atherosclerosis,myocardial infarction,ischemic stroke,abdominal aortic aneurysm,and myocarditis.Collectively,this integrated nanotheranostic paradigm establishes a robust foundation for the next generation of cardiovascular precision medicine.展开更多
Carbon nanotubes(CNTs)are widely used in various fields owing to their unique properties.In this study,three different types of nitrogen-doped CNT heterojunctions were constructed:parallel-doped(PCNT),vertically doped...Carbon nanotubes(CNTs)are widely used in various fields owing to their unique properties.In this study,three different types of nitrogen-doped CNT heterojunctions were constructed:parallel-doped(PCNT),vertically doped(VCNT),and mesh-doped(MCNT).Non-equilibrium molecular dynamics(NEMD)simulations were conducted to investigate their heat flux and thermal rectification(TR)effects.The results show that heat flux preferentially flows from nitrogen-doped regions to undoped regions,exhibiting distinct thermal rectification behavior,with PCNT showing the most pronounced effect.Interestingly,the TR ratio of the zigzag PCNT is significantly higher than that of the armchair PCNT.Subsequently,we examined the effects of system length and diameter on the TR ratio of the PCNT and found that the TR ratio increases and then decreases with increasing model length.In addition,the effect of defect density on the heat flux of the PCNT is peculiar.The phonon density of states,phonon dispersion,participation ratio,and phonon spectral heat flux were analyzed to elucidate the thermal transport behavior of phonons in the nanotubes.This study provides insights into the development and design of nitrogen-doped CNT thermal rectifiers.展开更多
In the context of power generation companies, vast amounts of specialized data and expert knowledge have been accumulated. However, challenges such as data silos and fragmented knowledge hinder the effective utilizati...In the context of power generation companies, vast amounts of specialized data and expert knowledge have been accumulated. However, challenges such as data silos and fragmented knowledge hinder the effective utilization of this information. This study proposes a novel framework for intelligent Question-and-Answer (Q&A) systems based on Retrieval-Augmented Generation (RAG) to address these issues. The system efficiently acquires domain-specific knowledge by leveraging external databases, including Relational Databases (RDBs) and graph databases, without additional fine-tuning for Large Language Models (LLMs). Crucially, the framework integrates a Dynamic Knowledge Base Updating Mechanism (DKBUM) and a Weighted Context-Aware Similarity (WCAS) method to enhance retrieval accuracy and mitigate inherent limitations of LLMs, such as hallucinations and lack of specialization. Additionally, the proposed DKBUM dynamically adjusts knowledge weights within the database, ensuring that the most recent and relevant information is utilized, while WCAS refines the alignment between queries and knowledge items by enhanced context understanding. Experimental validation demonstrates that the system can generate timely, accurate, and context-sensitive responses, making it a robust solution for managing complex business logic in specialized industries.展开更多
Chinese cabbage(Brassica rapa subsp.pekinensis)is a widely cultivated vegetable crop in Asia with significant economic importance(Li et al.,2024;Yu et al.,2024).As a potyvirus with the broad host range,turnip mosaic v...Chinese cabbage(Brassica rapa subsp.pekinensis)is a widely cultivated vegetable crop in Asia with significant economic importance(Li et al.,2024;Yu et al.,2024).As a potyvirus with the broad host range,turnip mosaic virus(TuMV)is a major pathogen affecting Chinese cabbages,leading to severe yield losses(Li et al.,2019).Traditional control measures have shown limited efficacy,and the long-term use of chemical pesticides has led to significant issues such as environmental pollution and pathogen resistance(Samara et al.,2021;Lu et al.,2022).Biologicallyderived pesticides have garnered considerable attention owing to their eco-friendly attributes(Ayilara et al.,2023).γ-Aminobutyric acid(GABA),initially discovered in potato tubers,has been proven to regulate immune responses and enhance resistance to fungal and bacterial pathogens by modulating reactive oxygen species and stress-related hormone signals(Tarkowski et al.,2020;Wang et al.,2025).But biologically-derived agents typically face challenges such as large particle size and instability,which limit their practical application and bioavailability(Daraban et al.,2023).展开更多
Aqueous aluminum ion batteries(AAIBs)have garnered extensive attention due to their environmental friendliness,high theoretical capacity,and low cost.However,the sluggish reaction kinetics and severe structural collap...Aqueous aluminum ion batteries(AAIBs)have garnered extensive attention due to their environmental friendliness,high theoretical capacity,and low cost.However,the sluggish reaction kinetics and severe structural collapse of the cathode material,especially manganese oxide,during the cycling process have hindered its further application.Herein,Cu^(2+)pre-interca la ted layeredδ-MnO_(2)was synthesized via a hydrothermal method.The pre-intercalated Cu^(2+)ions not only improve the conductivity of MnO_(2)cathode but also stabilize the structure to enhance stability.X-ray absorption fine structure(XAFS)combined with density functional theory(DFT)calculations confirm the formation of the covalent bond between Cu and O,increasing the electronegativity of O atoms and enhancing the H^(+)adsorption energy.Moreover,ex-situ measurements not only elucidate the Al^(3+)/H^(+)co-insertion energy storage mechanism but also demonstrate the high reversibility of the Cu-MnO_(2)cathode during cycling.This work provides a promising modification approach for the application of manganese oxides in AAIBs.展开更多
Tenebrionid beetles represent a crucial arthropod taxon in the Gobi desert ecosystems owing to their species richness and high biomass,both of which are essential for maintaining ecosystem health and stability.However...Tenebrionid beetles represent a crucial arthropod taxon in the Gobi desert ecosystems owing to their species richness and high biomass,both of which are essential for maintaining ecosystem health and stability.However,the spatiotemporal variations of tenebrionid beetle assemblages in the Gobi desert remain poorly understood.In this study,the monthly dynamics of tenebrionid beetles in the central part of the Hexi Corridor,Northwest China,a representative area of the Gobi desert ecosystems,were monitored using pitfall trapping during 2015-2020.The following results were showed:(1)monthly activity of tenebrionid beetles was observed from March to October,with monthly activity peaking in spring and summer,and monthly activity periods and peak of tenebrionid beetle species exhibited interspecific differences that varied from year to year;(2)spatial distribution of tenebrionid beetle community was influenced by structural factors.Specifically,at a spatial scale of 24.00 m,tenebrionid beetle community was strongly and positively correlated with the dominant species,with distinct spatial distribution patterns observed for Blaps gobiensis and Microdera kraatzi alashanica;(3)abundance of tenebrionid beetles was positively correlated with monthly mean precipitation and monthly mean temperature,whereas monthly abundance of B.gobiensis and M.kraatzi alashanica was positively correlated with monthly mean precipitation;and(4)the cover of Reaumuria soongarica(Pall.)Maxim.and Nitraria sphaerocarpa Maxim.had a positive influence on the number of tenebrionid beetles captured.In conclusion,monthly variation in precipitation significantly influences the community dynamic of tenebrionid beetles,with precipitation and shrub cover jointly determining the spatial distribution pattern of these beetles in the Gobi desert ecosystems.展开更多
[Objective]To construct an Escherichia coli mutant strain that accumulates pyruvate by genetic modification guided by the genome-scale metabolic network model.[Methods]Using a genome-scale metabolic network model as a...[Objective]To construct an Escherichia coli mutant strain that accumulates pyruvate by genetic modification guided by the genome-scale metabolic network model.[Methods]Using a genome-scale metabolic network model as a guide,we simulated pyruvate production of E.coli,screened key genes in metabolic pathways,and developed gene editing procedures accordingly.We knocked out the acetate kinase gene ackA,phosphate acetyltransferase gene pta,alcohol dehydrogenase adhE,glycogen synthase gene glgA,glycogen phosphorylase gene glgP,phosphoribosyl pyrophosphate(PRPP)synthase gene prs,ribose 1,5-bisphosphate phosphokinase gene phnN,and transporter encoding gene proP.Furthermore,we knocked in the transporter encoding gene ompC,flavonoid toxin gene fldA,and D-serine ammonia lyase gene dsdA.[Results]A shake flask process with the genetically edited mutant strain MG1655-6-2 under anaerobic conditions produced pyruvate at a titer of 10.46 g/L and a yield of 0.69 g/g.Metabolomic analysis revealed a significant increase in the pyruvate level in the fermentation broth,accompanied by notable decreases in the levels of certain related metabolic byproducts.Through 5 L fed-batch fermentation and an adaptive laboratory evolution,the strain finally achieved a pyruvate titer of 45.86 g/L.[Conclusion]This study illustrated the efficacy of a gene editing strategy predicted by a genome-scale metabolic network model in enhancing pyruvate accumulation in E.coli under anaerobic conditions and provided novel insights for microbial metabolic engineering.展开更多
Propane dehydrogenation(PDH)has emerged as a key on-purpose technology for the production of propylene,but it often depends on toxic chromium and expensive platinum catalysts,highlighting the need for environmentally ...Propane dehydrogenation(PDH)has emerged as a key on-purpose technology for the production of propylene,but it often depends on toxic chromium and expensive platinum catalysts,highlighting the need for environmentally friendly and cost-effective alternatives.In this study,we developed a facile impregnation method to fabricate unsaturated Co single-atoms with a tricoordinated Co_(1)O_(3)H_(x) structure by regulating silanol nests in purely siliceous Beta zeolites.Detailed PDH catalytic tests and characterizations revealed a positive correlation between the presence of silanol nests and enhanced catalytic activity.Additionally,the unsaturated Co single-atoms exhibited a carbon deposition rate more than an order of magnitude slower than that of Co nanoparticles.Notably,the optimized Co_(0.3%)/deAl-meso-Beta catalyst achieved a record-high propylene formation rate of 21.2 mmol_(C3H6) g_(cat)^(-1) h^(-1),with an exceptional propylene selectivity of 99.1%at 550℃.Moreover,the Co_(0.3%)/deAl-meso-Beta catalyst demonstrated excellent stability,with negligible deactivation after 5 consecutive regeneration cycles.This study emphasizes the pivotal role of silanol nests of zeolites in stabilizing and modulating the coordination environment of metallic active sites,providing valuable insights for the design of high-activity,high-stability,and low-cost PDH catalysts.展开更多
BACKGROUND Primary biliary cholangitis(PBC)is a chronic autoimmune-mediated cholestatic liver disease.Nanoparticles encapsulating rapamycin(ImmTOR)suppress adaptive immune responses and induce the hepatic tolerogenic ...BACKGROUND Primary biliary cholangitis(PBC)is a chronic autoimmune-mediated cholestatic liver disease.Nanoparticles encapsulating rapamycin(ImmTOR)suppress adaptive immune responses and induce the hepatic tolerogenic immune response.AIM To investigate the effects of ImmTOR in PBC mouse models.METHODS PBC models were induced in C57BL/6 mice by two immunizations of 2-octynoic acid-coupled bovine serum albumin at two-week intervals,and polycytidylic acid every three days.The PBC mouse models were separated into the treatment group and the control group.The levels of alkaline phosphatase(ALP)and alanine aminotransferase in the mice were detected using an automatic biochemical analyzer.Liver and spleen mononuclear cells were analyzed by flow cytometry,and serum anti-mitochondrial antibodies(AMA)and the related cytokines were analyzed by enzyme-linked immunosorbent assay.Liver histopathology was examined by hematoxylin and eosin staining and scored.RESULTS After treatment with ImmTOR,the ALP level was significantly decreased(189.60 U/L±27.25 U/L vs 156.00 U/L±17.21 U/L,P<0.05),the level of AMA was reduced(1.28 ng/mL±0.27 ng/mL vs 0.56 ng/mL±0.07 ng/mL,P<0.001)and the expression levels of interferon gamma and tumor necrosis factorαwere significantly decreased(48.29 pg/mL±10.84 pg/mL vs 25.01 pg/mL±1.49 pg/mL,P<0.0001)and(84.24 pg/mL±23.47 pg/mL vs 40.66 pg/mL±14.65 pg/mL,P<0.001).The CD4+T lymphocytes,CD8+T lymphocytes and B lymphocytes in the liver were significantly reduced,with statistically significant differences(24.21%±6.55%vs 15.98%±3.03%,P<0.05;9.09%±1.91%vs 5.49%±1.00%,P<0.001;80.51%±2.96%vs 75.31%±4.34%,P<0.05).The expression of CD8+T lymphocytes and B lymphocytes in the ImmTOR treatment group also decreased(9.09%±1.91%vs 5.49%±1.00%,P<0.001;80.51%±2.96%vs 75.31%±4.34%,P<0.05).The liver pathology of PBC mice in the treatment group showed reduced inflammation and a decreased total pathology score,and the difference in the scores was statistically significant(4.50±2.88 vs 1.75±1.28,P<0.05).CONCLUSION ImmTOR can improve biochemistry and pathology of liver obvious by inhibiting the expression of CD8+T cells and B cells,and reducing the titer of AMA.展开更多
The preparation of immobilized enzyme with excellent performance is one of the difficulties that restrict the application of enzyme catalysis technology.Here,Candida rugosa lipase(CRL)was firstly adsorbed on the surfa...The preparation of immobilized enzyme with excellent performance is one of the difficulties that restrict the application of enzyme catalysis technology.Here,Candida rugosa lipase(CRL)was firstly adsorbed on the surface of magnetic zeolitic imidazolate framework-8(ZIF-8)nanospheres,which was further encapsulated with a mesoporous SiO_(2)nano-membrane formed by tetraethyl orthosilicate(TEOS)polycondensation.Consequently,lipase could be firmly immobilized on carrier surface by physical binding rather than chemical binding,which did not damage the active conformation of enzyme.There were mesopores on the silica nano-membrane,which could improve the accessibility of enzyme and its apparent catalytic activity.Moreover,silica membrane encapsulation could also improve the stability of enzyme,suggesting an effective enzyme immobilization strategy.It showed that TEOS amount and the encapsulation time had significant effects on the thickness of silica membrane and the enzyme activity.The analysis in enzyme activity and protein secondary structure showed that lipase encapsulated in silica membrane retained the active conformation to the greatest extent.Compared with the adsorbed lipase,the encapsulated lipase increased its thermostability by 3 times and resistance to chemical denaturants by 7 times.The relative enzyme activity remained around 80%after 8 repetitions,while the adsorbed lipase only remained at7.3%.展开更多
Utilizing data from 5,481 village-level service stations(VS)in Baoding,China,this study empirically investigates the impact of digital inclusive finance(DIF)and VS infrastructure on rural e-commerce(RE)development.Res...Utilizing data from 5,481 village-level service stations(VS)in Baoding,China,this study empirically investigates the impact of digital inclusive finance(DIF)and VS infrastructure on rural e-commerce(RE)development.Results demonstrate that DIF significantly promotes RE by reducing transaction and financing costs(β=0.783,P<0.01).VS as physical infrastructure significantly drives RE growth(β=0.654,P<0.01).Crucially,DIF and VS exhibit synergistic effects(interactionβ=0.421,P<0.01),producing a“1+1>2”enhancement.Regional economic development,population density,and education levels positively influence RE.Current challenges include financial misallocation,infrastructure deficits,and skill gaps.We propose a“One Core,Three Wings”framework—Core:Develop tailored financial products for specialty industries(luggage,textiles,mushrooms);Wings:(1)Upgrade digital infrastructure,(2)Optimize policy synergy,(3)Enhance inclusive capacity through elderly-friendly technologies and training.Implementation strategies include establishing risk-sharing mechanisms,expanding VS functionality,and creating DIF-RE-VS alliances to foster sustainable rural revitalization.展开更多
Objective:To explore the effect of comprehensive nursing on relieving psychological pressure in patients with pulmonary micro-nodules.Methods:One hundred and twenty patients with pulmonary micro-nodules who received d...Objective:To explore the effect of comprehensive nursing on relieving psychological pressure in patients with pulmonary micro-nodules.Methods:One hundred and twenty patients with pulmonary micro-nodules who received diagnosis and treatment in our hospital from January 2024 to December 2024 were randomly divided into control and observation groups.The control group received routine nursing,while the observation group received comprehensive nursing.The Self-Rating Anxiety Scale(SAS)and Self-rating Depression Scale(SDS)were used to evaluate the patients’psychological states before and after nursing.Patient satisfaction with nursing was analyzed using a nursing satisfaction survey scale.Results:After nursing,the SAS and SDS scores of the observation group were significantly lower than those of the control group(P<0.05).The nursing satisfaction rates of the observation and control groups were 98.33%and 83.33%,respectively,with statistically significant differences(P<0.05).Conclusion:Comprehensive nursing intervention for patients with pulmonary micro-nodules can effectively relieve their psychological pressure and improve nursing satisfaction,demonstrating clinical value.展开更多
文摘Iris domestica,a perennial herb of the Iridaceae family,is widely recognized for its rich isoflavone content and broad therapeutic properties.To elucidate the biosynthetic pathway of these medicinally significant compounds,we constructed a haplotype-resolved genome assembly of this species.Transcriptomic and metabolomic analyses revealed tissue-specific accumulation of isoflavone,particularly in rhizomes and roots.Functional characterization identified two candidate isoflavone synthase genes,among which IdIFS was confirmed to promote the biosynthesis of key compounds tectorigenin and irisflorentin.The high-quality genome assembly presented here provides a foundational resource for further research into the evolution,secondary metabolite,and environmental adaptation of I.domestica.
基金Baoding Science and Technology Plan Project(Project No.:2341ZF214)。
文摘Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100 COPD subjects from our hospital from January 2020 to December 2023 were included and randomly divided into a healthy control group and an experimental group(50 cases each).The healthy control group underwent slow vital capacity measurement using a spirometer,while the experimental group underwent high-resolution thin-slice CT scans and serum Vanin-1 and SPP1 concentration measurements.Pulmonary function parameters,symptom burden,biomarker concentrations,and imaging characteristics were compared between the two groups.Results:The FEV1/FVC ratio in the experimental group(58.3±7.2)was lower than that in the healthy control group(92.1±4.8);the total CAT score(22.4±3.5)was higher than that in the healthy control group(3.1±1.2);both Vanin-1(18.7±2.3μg/L)and SPP1(25.6±4.1μg/L)levels were higher than those in the healthy control group;LAA%-950(38.7±6.2%)and WA%(68.5±5.3%)were significantly higher than those in the healthy control group(all p<0.001).Conclusion:Chest CT combined with serum Vanin-1 and SPP1 can accurately quantify the pathological progression of COPD,providing a dual basis for clinical staging and individualized intervention.
基金supported by Hebei Natural Science Foundation(No.JZX2023018)Hebei Natural Science Foundation(No.C2022201042)the 100 Foreign Experts Plans of Hebei Province(No.606080123001).
文摘Antibiotics are widespread in aquatic environments due to their extensive use in human healthcare and ani-mal husbandry.However,research on the occurrence and bioaccumulation of antibiotics in aquatic organisms within shallow wetland lakes remains limited.This study investigated the occurrence and bioaccumulation of ten commonly used antibiotics in the Baiyang Lake,northern China’s largest shallow wetland lake.The results indicated that sulfonamides and fluoroquinolones were the predominant antibiotics in surface water,whereas fluoroquinolones and macrolides were more prevalent in sediment.Fluoroquinolones demonstrated significant potential for bioaccumulation in targeted aquatic organisms,including both animals and plants(Carassius au-ratus and Phragmites australis).The bioaccumulation of antibiotics in Carassius auratus was correlated with their solubility,whereas in Phragmites australis,this was associated with their octanol-water partition coefficients and molecular weights.Ecological risk assessment indicated that most antibiotics posed minimal to low risk levels.However,four antibiotics were exceptions:clarithromycin(12.5%)and sulfamethoxazole(6.25%)presented a high risk in surface water samples,while norfloxacin(25.0%)and ciprofloxacin(25.0%)posed a high risk in sediment samples.Norfloxacin,ciprofloxacin,and roxithromycin were identified as key indicator antibiotics for enhancing the local monitoring and control of antibiotic contamination based on four criteria:(1)high con-centrations,(2)frequent detection,(3)capacity for bioaccumulation,and(4)ecological risk levels.This study contributes to a deeper understanding of the status of antibiotic contamination,bioaccumulation characteristics,and ecological risk in Baiyang Lake,thereby supporting efforts to monitor and regulate antibiotic pollution.
基金financially supported by the National Natural Science Foundation of China (No. 22579095)the Beijing-Tianjin-Hebei Basic Research Cooperation Special Project (B2024204027)+2 种基金the Youth Top-notch Talent Foundation of Hebei Provincial Universities (BJK2022023)the Natural Science Foundation of Hebei Province (B2023204006)the talent training project of Hebei province (No. B20231004)。
文摘The rate capability and cycling stability of sodium metal batteries taking FeS_(2) or sulfur as cathode are limited due to their low reaction kinetics and severe shuttle effect.Herein,we rationally design a novel single-atom-dispersed S_(2)-FeNC/FeS_(2) nanocluster heterojunction embedded in carbon spheres(SFNC/FeS_(2)) for the electrode material of sodium metal batteries.Interestingly,during the discharging process,the Na^(+) is inserted into FeS_(2) to generate Na_(2)S,as well as the unique electrochemical reaction between S_(2)-FeNC and Na^(+) to form Na_(2)S.Meanwhile,the FeNC can adsorb Na_(2)S and catalyze the conversion from Na_(2)S and Fe to FeS_(2) or from Na_(2)S and FeNC to S_(2)-FeNC for suppressing the shuttle effect and promoting the distinct hybrid reversible electrochemical behavior,which improves performance tremendously.Notably,the SFNC/FeS_(2) electrode delivers a specific capacity of 338.7 mAh g^(-1) after superlong 2000 cycles at a current density of 5.0 A g^(-1) and achieves a high energy density of 430.1 Wh Kg^(-1) at a current density of 0.05 A g^(-1).This work presents a novel approach to studying sodium metal batteries with hybrid behavior for excellent high energy density and cycling stability.
基金supported by the Natural Science Foundation of Anhui Province(2408085MC058 and 2308085QC91)National Natural Science Foundation of China(32301783and U21A20214)+5 种基金Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS CSIAF-202303)Nanfan special project,CAAS(YYLH2309,YBXM2322,YYLH2401)Scientific Innovation 2030 Project(2022ZD0401703)CAAS Innovative Team Award,Science and Technology of Innovative research program of Anhui Province(202423m1005002)National Key Research and Development Program of China(2023YFD1200900)the Natural Science Foundation General Program of Hebei Province(C2024204242).
文摘IR64 is an elite Xian/indica variety developed by International Rice Research Institute(IRRl)in 1985,which has been the most widely grown variety and core breeding parent in South/Southeast Asia(Mackill and Khush,2018).IR64 has been utilized to develop stress-tolerant(such as drought-adapted and submergenceresistant)near-isogenic lines,underscoring its great potential in agricultural genomics(Tanaka et al.,2020).
基金financial y supported by the National Key Research and Development Program of China (2023YFD1900902)the Joint Funds of the Zhejiang Provincial Natural Science Foundation of China (LLSSZ24C030001)+1 种基金the Earmarked Fund for China Agriculture Research System (CARS-08-G-09)sponsored by the K.C.Wong Magna Fund of Ningbo University,China。
文摘Fungi play crucial roles in nutrient acquisition,plant growth promotion,and the enhancement of resistance to both abiotic and biotic stresses.However,studies on the fungal communities associated with peas (Pisum sativum L.) remain limited.In this study,we systematically investigated the ecological effects of host niches (soil,root,stem,leaf,and pod) and genotypes on the diversity and composition of fungal communities in peas using a multi-level approach that encompassed pattern recognition (β-diversity decomposition),mechanism validation (neutral community model testing),and dynamic tracking methods (migration pathway source-tracking).The results revealed that the dominant fungal phyla across niches and genotypes were Ascomycota,Basidiomycota,and Mortierellomycota,and the community structures of the soil–plant continuum were primarily determined by the pea niches rather than genotypes.β-diversity decomposition was largely attributed to species replacement rather than richness differences,indicating strong niche specificity and microbial replacement across microhabitats.Neutral model analysis revealed that stochastic processes influenced genotypeassociated communities,while deterministic processes played a dominant role in niche-based community assembly.Source-tracking analysis identified niche-to-niche fungal migration,with Erysiphe,Fusarium,Cephaliophora,Ascobolus,Alternaria,and Aspergillus as the key genera.Migration rates from exogenous to endogenous niches were low (1.3–61.5%),whereas those within exogenous (64.4–83.7%) or endogenous (73.9–96.4%) compartments were much higher,suggesting that the pea epidermis acts as a selective barrier that filters and enriches microbial communities prior to internal colonization.This study provides comprehensive insights into the mechanisms of host filtering,enrichment and microbial sourcing,which increases our understanding of the assembly rules of the pea-associated fungal microbiome.
基金supported by the National Natural Science Foundation of China(Nos.42430503 and 42271048)the Hebei Natural Science Foundation(No.D2025205003)the Science Foundation of Hebei Normal University(No.L2025B31)。
文摘Non-structural carbohydrates(NSCs)are crucial for tree growth and survival under climatic stress,yet their spatial dynamics across broad climate gradients remain unclear.Pines(Pinus spp.),one of the most widely distributed tree genera worldwide,provide an ideal system for investigating large-scale spatial patterns of NSC within a single genus along extensive climatic gradients.Here,we compiled a global NSC database for pines across 74 sites,and assessed the spatial variation in total NSC,starch(St),and soluble sugars(SS)concentrations in stem sapwood,the primary reserve tissue,along site-specific mean annual temperature(MAT)and precipitation(MAP).Our results show that MAP exerted a stronger influence(R^(2)=20%–47%)on the spatial variation in total NSC and its components than did MAT(R^(2)=6%–16%).Tota concentrations declined nonlinearly with increasing MAP,with the rate of decline slowing beyond approximately 800 mm.While MAT had weaker effects on total NSC concentrations,both MAT and MAP jointly regulated NSC partitioning:Higher MAT and MAP were associated with reduced St concentrations but elevated SS concentrations and SS:St ratios.These findings suggest that pine species in cold and arid environments prioritize storing NSC as St,whereas in relatively warm and humid environments,NSC are preferentially mobilized into SS to support immediate metabolic and growth demands.
基金supported by the Bill&Melinda Gates Foundation(African cassava whitefly project,OPP1058938)the National Natural Science Foundation of China(Grant numbers 31501878).
文摘Bemisia tabaci is a complex of cryptic agro-economically important pest species characterized by diverse clades,substantial genetic diversity along with strong phylogeographic associations.However,a comprehensive phylogenomic analysis across the entire complex has been lacking,we thus conducted phylogenomic analyses and explored biogeographic patterns using 680 single-copy nuclear genes(SCNs)obtained from whole-genome sequencing data of 58 globally sourced B.tabaci specimens.We constructed both concatenation and coalescent trees using 680 SCNs,which produced highly supported bootstrap values and nearly identical topologies for all major clades.When comparing these concatenation trees with those constructed using mitochondrial cytochrome oxidase I(mtCOI)and mitochondrial genome,we found conflicting phylogenetic relationships,with the later trees recovering fewer major clades.In a separate comparison between concatenation and coalescent trees,particularly those generated using IQ-TREE,they were found to delineate population relationships more effectively than RaxML.In contrast,coalescent phylogenies were proficient in elucidating geographical dispersal patterns and the reorganization of biological species.Furthermore,we provided a strict consensus tree that clearly defines relationships within most clades,laying a solid foundation for future research on the evolution and taxonomy of B.tabaci.Ancestral range estimates suggested that the ancestral region of the complex is likely situated in equatorial Africa,the Middle East,and Mediterranean regions.Subsequently,the expansion occurred into part of the Palearctic and further into the Nearctic,Neotropical,Indomalayan,and Australasian regions.These findings challenge both previous classifications and origin hypotheses,offering a notably more comprehensive understanding of the global distribution,evolutionary history,diversification,and biogeography of B.tabaci.
基金supported by the National Natural Science Foundation of China(32371477,82090051,82301104,and 82300345)the National Key Research and Development Program of China(2021YFA1201000,2023YFC2605000)+2 种基金the National Natural Science Foundation of China Key Project(82430067,32030060)the Natural Science Foundation of Jiangsu Province(BK20230160)the Fundamental Research Funds for the Central Universities,Peking Union Medical College(3332025033).
文摘Immunotherapy for cardiovascular diseases(CVDs)holds great promise for precision management by modulating localized immune-inflammatory responses.The interplay between focal cardiovascular pathology and panvascular disease,necessitates highly integrated therapeutic strategies.Nano-technology-based theranostic platforms address this challenge by enabling both regulation and real-time imaging of immune cell activity within cardiovascular lesions.These functional nanotherapy systems not only halt disease progression at pathological sites but also reduce secondary cardiovascular events driven by shared inflammatory mechanisms.Additionally,nanoplatform-based dynamic visualization of immune cell responses facilitates adaptive,personalized interventions.This review introduces the role of immune cells in CVDs.It summarizes recent advances in nanomaterial-based immunomodulation strategies,including mechanisms of immune regulation,enhanced imaging,and therapeutic applications in atherosclerosis,myocardial infarction,ischemic stroke,abdominal aortic aneurysm,and myocarditis.Collectively,this integrated nanotheranostic paradigm establishes a robust foundation for the next generation of cardiovascular precision medicine.
基金supported by the National Natural Science Foundation of China(Grant No.52476071)the Natural Science Foundation of Hebei Province(Grant No.A2024502008).
文摘Carbon nanotubes(CNTs)are widely used in various fields owing to their unique properties.In this study,three different types of nitrogen-doped CNT heterojunctions were constructed:parallel-doped(PCNT),vertically doped(VCNT),and mesh-doped(MCNT).Non-equilibrium molecular dynamics(NEMD)simulations were conducted to investigate their heat flux and thermal rectification(TR)effects.The results show that heat flux preferentially flows from nitrogen-doped regions to undoped regions,exhibiting distinct thermal rectification behavior,with PCNT showing the most pronounced effect.Interestingly,the TR ratio of the zigzag PCNT is significantly higher than that of the armchair PCNT.Subsequently,we examined the effects of system length and diameter on the TR ratio of the PCNT and found that the TR ratio increases and then decreases with increasing model length.In addition,the effect of defect density on the heat flux of the PCNT is peculiar.The phonon density of states,phonon dispersion,participation ratio,and phonon spectral heat flux were analyzed to elucidate the thermal transport behavior of phonons in the nanotubes.This study provides insights into the development and design of nitrogen-doped CNT thermal rectifiers.
文摘In the context of power generation companies, vast amounts of specialized data and expert knowledge have been accumulated. However, challenges such as data silos and fragmented knowledge hinder the effective utilization of this information. This study proposes a novel framework for intelligent Question-and-Answer (Q&A) systems based on Retrieval-Augmented Generation (RAG) to address these issues. The system efficiently acquires domain-specific knowledge by leveraging external databases, including Relational Databases (RDBs) and graph databases, without additional fine-tuning for Large Language Models (LLMs). Crucially, the framework integrates a Dynamic Knowledge Base Updating Mechanism (DKBUM) and a Weighted Context-Aware Similarity (WCAS) method to enhance retrieval accuracy and mitigate inherent limitations of LLMs, such as hallucinations and lack of specialization. Additionally, the proposed DKBUM dynamically adjusts knowledge weights within the database, ensuring that the most recent and relevant information is utilized, while WCAS refines the alignment between queries and knowledge items by enhanced context understanding. Experimental validation demonstrates that the system can generate timely, accurate, and context-sensitive responses, making it a robust solution for managing complex business logic in specialized industries.
基金supported by the National Natural Science Foundation of China(Grant Nos.32402564,32330096,32372631)Hebei Natural Science Foundation(Grant No.C2024204246)+1 种基金the Pinduoduo-China Agricultural University Research Fund(Grant No.PC2023B02018)the 2115 Talent Development Program of China Agricultural University.
文摘Chinese cabbage(Brassica rapa subsp.pekinensis)is a widely cultivated vegetable crop in Asia with significant economic importance(Li et al.,2024;Yu et al.,2024).As a potyvirus with the broad host range,turnip mosaic virus(TuMV)is a major pathogen affecting Chinese cabbages,leading to severe yield losses(Li et al.,2019).Traditional control measures have shown limited efficacy,and the long-term use of chemical pesticides has led to significant issues such as environmental pollution and pathogen resistance(Samara et al.,2021;Lu et al.,2022).Biologicallyderived pesticides have garnered considerable attention owing to their eco-friendly attributes(Ayilara et al.,2023).γ-Aminobutyric acid(GABA),initially discovered in potato tubers,has been proven to regulate immune responses and enhance resistance to fungal and bacterial pathogens by modulating reactive oxygen species and stress-related hormone signals(Tarkowski et al.,2020;Wang et al.,2025).But biologically-derived agents typically face challenges such as large particle size and instability,which limit their practical application and bioavailability(Daraban et al.,2023).
基金financially supported by the National Natural Science Foundation of China(52102233)Science and Technology Project of Hebei Education Department(QN2023019)。
文摘Aqueous aluminum ion batteries(AAIBs)have garnered extensive attention due to their environmental friendliness,high theoretical capacity,and low cost.However,the sluggish reaction kinetics and severe structural collapse of the cathode material,especially manganese oxide,during the cycling process have hindered its further application.Herein,Cu^(2+)pre-interca la ted layeredδ-MnO_(2)was synthesized via a hydrothermal method.The pre-intercalated Cu^(2+)ions not only improve the conductivity of MnO_(2)cathode but also stabilize the structure to enhance stability.X-ray absorption fine structure(XAFS)combined with density functional theory(DFT)calculations confirm the formation of the covalent bond between Cu and O,increasing the electronegativity of O atoms and enhancing the H^(+)adsorption energy.Moreover,ex-situ measurements not only elucidate the Al^(3+)/H^(+)co-insertion energy storage mechanism but also demonstrate the high reversibility of the Cu-MnO_(2)cathode during cycling.This work provides a promising modification approach for the application of manganese oxides in AAIBs.
基金funded by the National Natural Science Foundation of China(U23A2063)the Gansu Province Top-notch Leading Talents Project(E339040101)the National Natural Science Foundation of China(41771290,42377043,41773086).
文摘Tenebrionid beetles represent a crucial arthropod taxon in the Gobi desert ecosystems owing to their species richness and high biomass,both of which are essential for maintaining ecosystem health and stability.However,the spatiotemporal variations of tenebrionid beetle assemblages in the Gobi desert remain poorly understood.In this study,the monthly dynamics of tenebrionid beetles in the central part of the Hexi Corridor,Northwest China,a representative area of the Gobi desert ecosystems,were monitored using pitfall trapping during 2015-2020.The following results were showed:(1)monthly activity of tenebrionid beetles was observed from March to October,with monthly activity peaking in spring and summer,and monthly activity periods and peak of tenebrionid beetle species exhibited interspecific differences that varied from year to year;(2)spatial distribution of tenebrionid beetle community was influenced by structural factors.Specifically,at a spatial scale of 24.00 m,tenebrionid beetle community was strongly and positively correlated with the dominant species,with distinct spatial distribution patterns observed for Blaps gobiensis and Microdera kraatzi alashanica;(3)abundance of tenebrionid beetles was positively correlated with monthly mean precipitation and monthly mean temperature,whereas monthly abundance of B.gobiensis and M.kraatzi alashanica was positively correlated with monthly mean precipitation;and(4)the cover of Reaumuria soongarica(Pall.)Maxim.and Nitraria sphaerocarpa Maxim.had a positive influence on the number of tenebrionid beetles captured.In conclusion,monthly variation in precipitation significantly influences the community dynamic of tenebrionid beetles,with precipitation and shrub cover jointly determining the spatial distribution pattern of these beetles in the Gobi desert ecosystems.
基金supported by the Hebei Provincial Key Research and Development Project(21372803D)。
文摘[Objective]To construct an Escherichia coli mutant strain that accumulates pyruvate by genetic modification guided by the genome-scale metabolic network model.[Methods]Using a genome-scale metabolic network model as a guide,we simulated pyruvate production of E.coli,screened key genes in metabolic pathways,and developed gene editing procedures accordingly.We knocked out the acetate kinase gene ackA,phosphate acetyltransferase gene pta,alcohol dehydrogenase adhE,glycogen synthase gene glgA,glycogen phosphorylase gene glgP,phosphoribosyl pyrophosphate(PRPP)synthase gene prs,ribose 1,5-bisphosphate phosphokinase gene phnN,and transporter encoding gene proP.Furthermore,we knocked in the transporter encoding gene ompC,flavonoid toxin gene fldA,and D-serine ammonia lyase gene dsdA.[Results]A shake flask process with the genetically edited mutant strain MG1655-6-2 under anaerobic conditions produced pyruvate at a titer of 10.46 g/L and a yield of 0.69 g/g.Metabolomic analysis revealed a significant increase in the pyruvate level in the fermentation broth,accompanied by notable decreases in the levels of certain related metabolic byproducts.Through 5 L fed-batch fermentation and an adaptive laboratory evolution,the strain finally achieved a pyruvate titer of 45.86 g/L.[Conclusion]This study illustrated the efficacy of a gene editing strategy predicted by a genome-scale metabolic network model in enhancing pyruvate accumulation in E.coli under anaerobic conditions and provided novel insights for microbial metabolic engineering.
文摘Propane dehydrogenation(PDH)has emerged as a key on-purpose technology for the production of propylene,but it often depends on toxic chromium and expensive platinum catalysts,highlighting the need for environmentally friendly and cost-effective alternatives.In this study,we developed a facile impregnation method to fabricate unsaturated Co single-atoms with a tricoordinated Co_(1)O_(3)H_(x) structure by regulating silanol nests in purely siliceous Beta zeolites.Detailed PDH catalytic tests and characterizations revealed a positive correlation between the presence of silanol nests and enhanced catalytic activity.Additionally,the unsaturated Co single-atoms exhibited a carbon deposition rate more than an order of magnitude slower than that of Co nanoparticles.Notably,the optimized Co_(0.3%)/deAl-meso-Beta catalyst achieved a record-high propylene formation rate of 21.2 mmol_(C3H6) g_(cat)^(-1) h^(-1),with an exceptional propylene selectivity of 99.1%at 550℃.Moreover,the Co_(0.3%)/deAl-meso-Beta catalyst demonstrated excellent stability,with negligible deactivation after 5 consecutive regeneration cycles.This study emphasizes the pivotal role of silanol nests of zeolites in stabilizing and modulating the coordination environment of metallic active sites,providing valuable insights for the design of high-activity,high-stability,and low-cost PDH catalysts.
基金Supported by Cultivation Project of Hebei Natural Science Foundation-Precision Medicine Joint Fund,No.H2021206239.
文摘BACKGROUND Primary biliary cholangitis(PBC)is a chronic autoimmune-mediated cholestatic liver disease.Nanoparticles encapsulating rapamycin(ImmTOR)suppress adaptive immune responses and induce the hepatic tolerogenic immune response.AIM To investigate the effects of ImmTOR in PBC mouse models.METHODS PBC models were induced in C57BL/6 mice by two immunizations of 2-octynoic acid-coupled bovine serum albumin at two-week intervals,and polycytidylic acid every three days.The PBC mouse models were separated into the treatment group and the control group.The levels of alkaline phosphatase(ALP)and alanine aminotransferase in the mice were detected using an automatic biochemical analyzer.Liver and spleen mononuclear cells were analyzed by flow cytometry,and serum anti-mitochondrial antibodies(AMA)and the related cytokines were analyzed by enzyme-linked immunosorbent assay.Liver histopathology was examined by hematoxylin and eosin staining and scored.RESULTS After treatment with ImmTOR,the ALP level was significantly decreased(189.60 U/L±27.25 U/L vs 156.00 U/L±17.21 U/L,P<0.05),the level of AMA was reduced(1.28 ng/mL±0.27 ng/mL vs 0.56 ng/mL±0.07 ng/mL,P<0.001)and the expression levels of interferon gamma and tumor necrosis factorαwere significantly decreased(48.29 pg/mL±10.84 pg/mL vs 25.01 pg/mL±1.49 pg/mL,P<0.0001)and(84.24 pg/mL±23.47 pg/mL vs 40.66 pg/mL±14.65 pg/mL,P<0.001).The CD4+T lymphocytes,CD8+T lymphocytes and B lymphocytes in the liver were significantly reduced,with statistically significant differences(24.21%±6.55%vs 15.98%±3.03%,P<0.05;9.09%±1.91%vs 5.49%±1.00%,P<0.001;80.51%±2.96%vs 75.31%±4.34%,P<0.05).The expression of CD8+T lymphocytes and B lymphocytes in the ImmTOR treatment group also decreased(9.09%±1.91%vs 5.49%±1.00%,P<0.001;80.51%±2.96%vs 75.31%±4.34%,P<0.05).The liver pathology of PBC mice in the treatment group showed reduced inflammation and a decreased total pathology score,and the difference in the scores was statistically significant(4.50±2.88 vs 1.75±1.28,P<0.05).CONCLUSION ImmTOR can improve biochemistry and pathology of liver obvious by inhibiting the expression of CD8+T cells and B cells,and reducing the titer of AMA.
基金the financial supports from the National Natural Science Foundation of China(Nos.22378093,21878065)Natural Science Foundation of Hebei Province,China(No.E2022201100)+2 种基金the Science and Technology Support Plan of Baoding City(No.2241ZF111)the Medical Science Foundation of Hebei University(No.2021A09)the Foundation of Affiliated Hospital of Hebei University(No.2021Z003)。
文摘The preparation of immobilized enzyme with excellent performance is one of the difficulties that restrict the application of enzyme catalysis technology.Here,Candida rugosa lipase(CRL)was firstly adsorbed on the surface of magnetic zeolitic imidazolate framework-8(ZIF-8)nanospheres,which was further encapsulated with a mesoporous SiO_(2)nano-membrane formed by tetraethyl orthosilicate(TEOS)polycondensation.Consequently,lipase could be firmly immobilized on carrier surface by physical binding rather than chemical binding,which did not damage the active conformation of enzyme.There were mesopores on the silica nano-membrane,which could improve the accessibility of enzyme and its apparent catalytic activity.Moreover,silica membrane encapsulation could also improve the stability of enzyme,suggesting an effective enzyme immobilization strategy.It showed that TEOS amount and the encapsulation time had significant effects on the thickness of silica membrane and the enzyme activity.The analysis in enzyme activity and protein secondary structure showed that lipase encapsulated in silica membrane retained the active conformation to the greatest extent.Compared with the adsorbed lipase,the encapsulated lipase increased its thermostability by 3 times and resistance to chemical denaturants by 7 times.The relative enzyme activity remained around 80%after 8 repetitions,while the adsorbed lipase only remained at7.3%.
文摘Utilizing data from 5,481 village-level service stations(VS)in Baoding,China,this study empirically investigates the impact of digital inclusive finance(DIF)and VS infrastructure on rural e-commerce(RE)development.Results demonstrate that DIF significantly promotes RE by reducing transaction and financing costs(β=0.783,P<0.01).VS as physical infrastructure significantly drives RE growth(β=0.654,P<0.01).Crucially,DIF and VS exhibit synergistic effects(interactionβ=0.421,P<0.01),producing a“1+1>2”enhancement.Regional economic development,population density,and education levels positively influence RE.Current challenges include financial misallocation,infrastructure deficits,and skill gaps.We propose a“One Core,Three Wings”framework—Core:Develop tailored financial products for specialty industries(luggage,textiles,mushrooms);Wings:(1)Upgrade digital infrastructure,(2)Optimize policy synergy,(3)Enhance inclusive capacity through elderly-friendly technologies and training.Implementation strategies include establishing risk-sharing mechanisms,expanding VS functionality,and creating DIF-RE-VS alliances to foster sustainable rural revitalization.
基金Science and Technology Support Program of Baoding City,Hebei Province(Project No.:2241ZF326)。
文摘Objective:To explore the effect of comprehensive nursing on relieving psychological pressure in patients with pulmonary micro-nodules.Methods:One hundred and twenty patients with pulmonary micro-nodules who received diagnosis and treatment in our hospital from January 2024 to December 2024 were randomly divided into control and observation groups.The control group received routine nursing,while the observation group received comprehensive nursing.The Self-Rating Anxiety Scale(SAS)and Self-rating Depression Scale(SDS)were used to evaluate the patients’psychological states before and after nursing.Patient satisfaction with nursing was analyzed using a nursing satisfaction survey scale.Results:After nursing,the SAS and SDS scores of the observation group were significantly lower than those of the control group(P<0.05).The nursing satisfaction rates of the observation and control groups were 98.33%and 83.33%,respectively,with statistically significant differences(P<0.05).Conclusion:Comprehensive nursing intervention for patients with pulmonary micro-nodules can effectively relieve their psychological pressure and improve nursing satisfaction,demonstrating clinical value.
