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The Abundance of the Remaining Mammalian Fauna and the Impacts of Hunting in a Biodiversity Hotspot’s Hotspot in the Atlantic Forest of North-Eastern Brazil
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作者 Éverton Renan de Andrade Melo José Ramon Gadelha +1 位作者 Leandro Ricardo Rodrigues de Lucena Antonio Rossano Mendes Pontes 《Open Journal of Ecology》 2025年第1期65-91,共27页
Although hunting in the north-eastern Atlantic forest of Brazil began more than 500 years ago, no study to date has evaluated its impacts on the region’s mammalian fauna. For one year we carried out diurnal and noctu... Although hunting in the north-eastern Atlantic forest of Brazil began more than 500 years ago, no study to date has evaluated its impacts on the region’s mammalian fauna. For one year we carried out diurnal and nocturnal surveys using the Line Transect method in seven forest fragments varying from 7.32 ha to 469.76 ha, within a 4000 ha forest island archipelago, in Pernambuco State, Atlantic forest of northeastern Brazil. We calculated species density, population size, biomass and synergetic biomass, and recorded direct and indirect human impacts along the study transects. We recorded 44 mammalian species, of which 45.5% (n = 20) went extinct through hunting. The smallest forest fragment had the lowest richness, diversity, population size, and total biomass. It also had no synergetic biomass. The largest fragment had the highest richness, total density, and population size. There was a statistically significant relationship between fragment area and number of gunshots heard and suspended hunting platforms found;between population size and gunshots heard, suspended hunting platforms, free-roaming and feral dogs, and between total density and free-roaming and feral dogs. After more than 500 years of colonization hunting is still devastating, with larger fragments being linked to more hunters. Higher mammal abundances attracted more free-roaming and feral dogs, which have adapted to hunt wildlife on their own. Unless we protect every single forest fragment and create sustainable landscapes, we will not be able to save this hotspot’s hotspot. 展开更多
关键词 Atlantic Forest MAMMALS ABUNDANCE Human Impact Extinction
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Microstructure features and mechanical/electrochemical behavior of directionally solidified Al−6wt.%Cu−5wt.%Ni alloy 被引量:5
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作者 Adilson Vitor RODRIGUES Thiago Soares LIMA +3 位作者 Talita Almeida VIDA Crystopher BRITO Amauri GARCIA Noé CHEUNG 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2021年第6期1529-1549,共21页
The effects of the addition of 5.0 wt.%Ni to an Al−6wt.%Cu alloy on the solidification cooling rate(T),growth rate(V_(L)),length scale of the representative phase of the microstructure,morphology/distribution of inter... The effects of the addition of 5.0 wt.%Ni to an Al−6wt.%Cu alloy on the solidification cooling rate(T),growth rate(V_(L)),length scale of the representative phase of the microstructure,morphology/distribution of intermetallic compounds(IMCs)and on the resulting properties were investigated.Corrosion and tensile properties were determined on samples solidified under a wide range of T along the length of a directionally solidified Al−6wt.%Cu−5.0wt.%Ni alloy casting.Experimental growth laws were derived relating the evolution of primary(λ_(1))and secondary(λ_(2))dendritic spacings with T and V_(L).The elongation to fracture(δ)and the ultimate tensile strength(σ_(U))were correlated with the inverse of the square root of λ_(1) along the length of the casting by Hall−Petch type experimental equations.The reinforcing effect provided by the addition of Ni in the alloy composition is shown to surpass that provided by the refinement of the dendritic microstructure.The highest corrosion resistance is associated with a microstructure formed by thin IMCs evenly distributed in the interdendritic regions,typical of samples that are solidified under higher T. 展开更多
关键词 Al−Cu−Ni alloys as-cast microstructures dendritic spacings tensile properties corrosion resistance
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Application of exploratory data analysis: HCA and PCA in the classification of bicarbonated and not bicarbonated groundwaters in the Western Amazon Region 被引量:1
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作者 Francisco S. P. dos Santos Panero Henrique E. B. S. Eduardo Bezerra da Silva Joao S. P. dos Santos Panero 《Chinese Journal Of Geochemistry》 EI CAS 2006年第B08期165-166,共2页
关键词 HCA PCA 亚马逊河 地下水 重碳酸盐
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BIM技术在建筑电气设计中的应用探讨 被引量:7
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作者 李东晋 莫文静 赵明 《现代建筑电气》 2017年第6期7-10,共4页
介绍了建筑信息模型(BIM)技术概念、发展和国内外研究现状,结合某实际项目实例,探讨了BIM技术在建筑电气设计中的应用,阐述了BIM技术在建筑电气设计领域中的优势和不足。提出BIM技术在建筑电气设计中的应用还处于起步阶段,但随着国家对... 介绍了建筑信息模型(BIM)技术概念、发展和国内外研究现状,结合某实际项目实例,探讨了BIM技术在建筑电气设计中的应用,阐述了BIM技术在建筑电气设计领域中的优势和不足。提出BIM技术在建筑电气设计中的应用还处于起步阶段,但随着国家对BIM的大力推动、配套技术标准的出台和设计软件的不断更新,BIM技术在建筑电气设计领域的应用将越来越广泛。 展开更多
关键词 BIM技术 建筑电气设计 碰撞检查 BIM软件
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Tailoring microstructure and microhardness of Zn-1wt.%Mg-(0.5wt.%Mn, 0.5wt.%Ca) alloys by solidification cooling rate 被引量:1
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作者 Talita A.VIDA Cássio A.P.SILVA +3 位作者 Thiago S.LIMA NoéCHEUNG Crystopher BRITO Amauri GARCIA 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2021年第4期1031-1048,共18页
Biodegradable Zn-based alloys, particularly Zn-Mg alloys with the addition of alloying elements, have been intensively investigated aiming to improve both mechanical properties and corrosion behavior. Since such prope... Biodegradable Zn-based alloys, particularly Zn-Mg alloys with the addition of alloying elements, have been intensively investigated aiming to improve both mechanical properties and corrosion behavior. Since such properties are strongly dependent on the alloy microstructure, any evaluation should commence on understanding the conditions influencing its formation. In this study, the effect of the solidification cooling rate on the microstructural evolution of Zn-1 wt.%Mg-(0.5 wt.%Ca, 0.5 wt.%Mn) alloys during transient solidification was investigated. The results show that the microstructures of both alloys have three phases in common: η-Zn dendritic matrix, intermetallic compounds(IMCs) Zn11Mg2, and Zn2 Mg in the eutectic mixture. MnZn9 and two Ca-bearing phases(CaZn11 and CaZn13) are associated with Mn and Ca additions, respectively. These additions are shown to refine the dendritic matrix and the eutectic mixture as compared to the Zn-1 wt.%Mg alloy. A correlation between cooling rate, dendritic or eutectic spacings was developed, thus permitting experimental growth laws to be proposed. Additionally, hardness tests were performed to evaluate the effects of additions of Ca and Mn. Experimental correlations between Vickers microhardness and secondary dendritic spacings were proposed, showing that the microstructural refinement and characteristic Ca and Mn based IMCs induce an increase in hardness as compared to the binary alloy. 展开更多
关键词 Zn-Mg-(Ca Mn)alloys SOLIDIFICATION cooling rate MICROSTRUCTURE MICROHARDNESS
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Characterization of atmospheric aerosol (PM10 and PM2.5) from a medium sized city in S?o Paulo state,Brazil 被引量:3
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作者 Bruno T.Franzin Filipe C.Guizellini +5 位作者 Diego V.de Babos Ossamu Hojo Ieda Ap.Pastre Mary R.R.Marchi Fernando L.Fertonani Cristina M.R.R.Oliveira 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2020年第3期238-251,共14页
Air pollution causes deleterious effects on human health with aerosols being among the most polluting agents.The objective of this work is the characterization of the PM2.5 and PM10 aerosol mass in the atmosphere.The ... Air pollution causes deleterious effects on human health with aerosols being among the most polluting agents.The objective of this work is the characterization of the PM2.5 and PM10 aerosol mass in the atmosphere.The methods of analysis include WD-XRF and EDS.Data were correlated with meteorological information and air mass trajectories(model HYSPLIT)by multivariate analysis.A morphological structural analysis was also carried out to identify the probable sources of atmospheric aerosols in the city of Sao Jose do Rio Preto,Brazil.The mean mass concentration values obtained were 24.54 μg/m^3 for PM10,above the WHO annual standard value of 20 μg/m^3 and 10.88 μg/m^3 for PM2.5 whose WHO recommended limit is10 μg/m^3.WD-XRF analysis of the samples revealed Si and Al as major components of the coarse fraction.In the fine fraction,the major elements were Al and S.The SEM-FEG characterization allowed identifying the morphology of the particles in agglomerates,ellipsoids and filaments in the PM10,besides spherical in the PM2.5.The analysis by EDS corroborated WD-XRF results,identifying the crustal elements,aluminosilicates and elements of anthropogenic origin in the coarse fraction.For the fine fraction crustal elements were also identified;aluminosilicates,black carbon and spherical particles(C and O) originating from combustion processes were predominant.The use of multivariate analysis to correlate air mass trajectories with the results of the morpho-structural characterization of the particulate matter allowed confirmation of the complex composition of the particles resulting from the combination of both local and long-distance sources. 展开更多
关键词 URBAN AEROSOL PM10 PM2.5 WD-XRF SEM-EDS
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Agronomic evaluation of Herbaspirillum seropedicae strain ZAE94 as an inoculant to improve maize yield in Brazil 被引量:2
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作者 Gabriela C.ALVES Carlos L.R.DOS SANTOS +5 位作者 Jerri E.ZILLI Fabio B.DOS REIS JUNIOR Ivanildo E.MARRIEL Farley A.da F.BREDA Robert M.BODDEY Veronica M.REIS 《Pedosphere》 SCIE CAS CSCD 2021年第4期583-595,共13页
Diazotrophic bacteria applied as a seed inoculant can improve the grain yield of several crops including maize. The current study aimed to test the agronomic efficiency and contribution of biological nitrogen fixation... Diazotrophic bacteria applied as a seed inoculant can improve the grain yield of several crops including maize. The current study aimed to test the agronomic efficiency and contribution of biological nitrogen fixation(BNF) of the endophytic diazotroph Herbaspirillum seropedicae strain ZAE94 to maize under field conditions. Eighteen field assays were conducted in four different locations during consecutive years on two hybrids and two varieties of maize in a random block design with four replicates using a peat-based inoculant. The inoculant containing the ZAE94 strain was applied without nitrogen(N)fertilization or with 40 kg N ha^(-1) and was compared to the application of 40 and 80 kg N ha^(-1) without inoculation. Crop productivity and N accumulation in the grain were evaluated in addition to ^(15)N natural abundance(δ^(15)N) to evaluate BNF in the treatments without N fertilization. Fertilization at 40 kg N ha^(-1) plus bacterial inoculation produced crop yields similar to the treatment with 80 kg N ha^(-1) and increased grain N content, especially in the off-season with 40 kg N ha^(-1). The inoculation treatments showed lower δ^(15)N values than the non-inoculated treatments, which was most evident in the off-season. The BNF contributed about 30% of N accumulated in plants inoculated with ZAE94. On average, 64% of the N fertilized plots showed an increase of the parameters evaluated in the inoculated treatments, compared with the control. Inoculation also increased root length, root volume, and leaf area, and these parameters were positively correlated with plant weight using a hydroponic assay. This study revealed that the application of H. seropedicae inoculant increased the amount of N in plants owing to BNF, and there is a better chance of yield response to inoculation under low N fertilizer application in the off-season. 展开更多
关键词 agronomic efficiency biological nitrogen fixation endophytic diazotroph inoculation 15N natural abundance plant growth-promoting bacteria
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Seasonal Patterns of Nitrogen and Phosphorus in the Litter and Soil Surface of Transitional Amazon Cerrado Forest, Brazil 被引量:1
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作者 J. de S. Nogueira L. Sanches +1 位作者 O.B. Pinto-Junior E.D. de Almeida 《Journal of Environmental Science and Engineering》 2011年第1期5-14,共10页
Measurements of litter production, and the surface litter pool were made over a 1 year period in a tropical transitional forest near Sinop, Mato Grosso Brazil with the aim of quantifying the seasonal variation of nitr... Measurements of litter production, and the surface litter pool were made over a 1 year period in a tropical transitional forest near Sinop, Mato Grosso Brazil with the aim of quantifying the seasonal variation of nitrogen and phosphorus in the litter and the annual contribution of nutrients to the soil. Average annual litterfall (+95% confidence interval (CI)) was 8.20 ton.ha^-1 year^-1 and forest floor litter mass was 58.63 ton'hal. Nitrogen and phosphorus in the forest floor litter mass was highest during the dry and dry-wet season, being 38% higher than in the wet and wet-dry season. Seasonal variation in the litter and concentration of nutrients was explained by seasonal variations in the climate, for example in the precipition and soil humidity. Average annual nitrogen and phosphorus concentrations in the forest floor mass were 17.24 ton.ha^-1 and 16.46 ton.ha^-1, respectively. The more significant forest floor mass fraction for returning soil nutrients was the leaves. The concentration of nutrients was higher in the soil superficial layer (at depths between 0-5 cm) than at depths between 30-70 cm, approximately 83% and 93% for total nitrogen and available phosphorus, respectively. 展开更多
关键词 Tropical forest litterpool nutrient cycling LITTERFALL
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Application of Extended Multiplicative Signal Correction to Short-Wavelength near Infrared Spectra of Moisture in Marzipan 被引量:1
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作者 Pedro dos Santos Panero Francisco dos Santos Panero +1 位作者 Joao dos Santos Panero Henrique Eduardo Bezerra da Silva 《Journal of Data Analysis and Information Processing》 2013年第3期30-34,共5页
Short-wavelength near infrared spectroscopy (SW-NIR) is a very rapid, versatile and precise technique, which can be used in many different situations and for very types of products and chemical compounds. Extended mul... Short-wavelength near infrared spectroscopy (SW-NIR) is a very rapid, versatile and precise technique, which can be used in many different situations and for very types of products and chemical compounds. Extended multiplicative signal correction (EMSC) is a modification of the standard MSC pre-processing method that allows the separation of physical light scattering effects from chemical (vibrational) light absorbance effects in spectra. In this paper, the EMSC is applied and compared with first derivate, second derivate, MSC and SNV in combination of PLSR to obtain robust models in terms of accuracy and predict ability with a reduced calibration data set using SW-NIR spectra of moisture in marzipan. The Extended Multiplicative Signal Correction—EMSC and combination methods provide the best results in terms of prediction ability and calibration SW-NIR spectra of moisture in marzipan. The best classification results were obtained by Extended Multiplicative Signal Correction followed by second derivates. 展开更多
关键词 EMSC PLSR SW-NIR Extended Multiplicative Signal Correction CHEMOMETRICS
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Application of HCA and PCA in the discrimination of not polluted tubular wells from tubular wells with incidence of anthropogenic pollution in Western Amazon Region
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作者 Francisco S. P. dos Santos Panero Joao S. P. dos Santos Panero Henrique E. B. S. Eduardo Bezerra da Silva 《Chinese Journal Of Geochemistry》 EI CAS 2006年第B08期165-165,共1页
关键词 PCA HCA 亚马逊河 管状井 地下水污染 水体污染
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中国辽西两件长尾翼龙的简单注释(英文)
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作者 Alexander W.A.KELLNER 《地球学报》 EI CAS CSCD 北大核心 2010年第S1期36-37,共2页
Pterosaur diversity in China has increased tre-mendously in the last two decades particularly due to discoveries made in deposits of western Liaoning.
关键词 辽西 翼龙 长尾 注释 简单
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Genome-wide association mapping in a sweet cherry germplasm collection(Prunus avium L.)reveals candidate genes for fruit quality traits
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作者 Armel S.L.Donkpegan Anthony Bernard +6 位作者 Teresa Barreneche José Quero-García Hélène Bonnet Mathieu Fouché Loïck Le Dantec Bénédicte Wenden Elisabeth Dirlewanger 《Horticulture Research》 SCIE CSCD 2023年第10期227-249,共23页
In sweet cherry(Prunus avium L.),large variability exists for various traits related to fruit quality.There is a need to discover the genetic architecture of these traits in order to enhance the efficiency of breeding... In sweet cherry(Prunus avium L.),large variability exists for various traits related to fruit quality.There is a need to discover the genetic architecture of these traits in order to enhance the efficiency of breeding strategies for consumer and producer demands.With this objective,a germplasm collection consisting of 116 sweet cherry accessions was evaluated for 23 agronomic fruit quality traits over 2-6 years,and characterized using a genotyping-by-sequencing approach.The SNP coverage collected was used to conduct a genome-wide association study using two multilocus models and three reference genomes.We identified numerous SNP-trait associations for global fruit size(weight,width,and thickness),fruit cracking,fruit firmness,and stone size,and we pinpointed several candidate genes involved in phytohormone,calcium,and cell wall metabolisms.Finally,we conducted a precise literature review focusing on the genetic architecture of fruit quality traits in sweet cherry to compare our results with potential colocalizations of marker-trait associations.This study brings new knowledge of the genetic control of important agronomic traits related to fruit quality,and to the development of marker-assisted selection strategies targeted towards the facilitation of breeding efforts. 展开更多
关键词 BREEDING GERMPLASM TRAITS
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Morphophysiology of Germination among Seeds of Pau-rainha (Centrolobium paraense) Collected in the State of Roraima
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作者 Luis Augusto Melo Schwengber Oscar Jose Smiderle +3 位作者 Aline das Gracas Souza Jose Maria Arcanjo Alves Dalton Roberto Schwengber Cylles Zara dos Reis Barbosa 《Journal of Agricultural Science and Technology(B)》 2016年第6期371-379,共9页
This work intended to characterize the seed morphology, water absorption patterns and to determine the vigor among different colored seeds of the species Centrolobium paraense (Fabaceae). For this purpose, samaras w... This work intended to characterize the seed morphology, water absorption patterns and to determine the vigor among different colored seeds of the species Centrolobium paraense (Fabaceae). For this purpose, samaras which were opened were collected from two sites---Agua Boa (AB) and Taiano (TAI) lo^ated in the state of Roraima, and the seeds screened by the coat coloration (light brown/intermediate brown/dark brown) for four conditions (two sites and two years of collection). Evaluation of water absorption by the seeds at different periods, mass of 1,000 seeds, water content, electrical conductivity (EC), seedling emergence and emergence velocity (EV) were performed. The water absorption by the seeds of Centrolobium paraense indicates coat permeability to water and distinct absorptions according to coat colorations. The EC test is efficient in determining the physiological quality of seeds of Centrolobium paraense. Seed coat coloration affects the physiological quality of the seeds of Centrolobium paraense. It is possible to distinguish the seeds of AB and TAI into two degrees of physiological quality, the light brown-colored seeds being more vigorous. 展开更多
关键词 Centrolobium paraense water absorption curve electrical conductivity seed vigor seed coloring.
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古翼手龙类的德国翼手龙新标本(英文)
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作者 Taissa RODRIGUES Alexander W.A.KELLNER Oliver W.M.RAUHUT 《地球学报》 EI CAS CSCD 北大核心 2010年第S1期57-58,共2页
The pterodactyloid genus Germanodactylus is rela-tively poorly known. Two species have been described, although it has been recently suggested (Wang et al., 2008)
关键词 翼手龙类 德国 标本
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Novel Cancer Treatment Using Targeted Delivery of Short-Lived Radiologically Activated Nanoparticles
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作者 P.L.Andrade J.B.Leao 《Journal of Health Science》 2019年第1期43-47,共5页
The goal of our preliminary study is to show that a minimal of overall body toxicity while maximizing the cancer tumor treatment may be achieved in a novel approach where minute amounts of coated nanoparticles may be ... The goal of our preliminary study is to show that a minimal of overall body toxicity while maximizing the cancer tumor treatment may be achieved in a novel approach where minute amounts of coated nanoparticles may be radiologically activated prior to treatment and“coerce”tumor cells into readily absorb these nanoparticles.This targeted intracytoplasmic delivery of short-lived radiologically activated nanoparticles could provide less“whole-body”radiation dose while delivering a short lived potent tumor localized dose,along with their low toxicity may prove to be another tool in the treatment of diverse cancers. 展开更多
关键词 NANOPARTICLES neutron activation IRRADIATION cancer metabolism WARBURG effect GLUTAMINE GLUCOSE
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Modelling Plant Disease Risk Areas Based on Brazilian Climate Change Scenarios
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作者 M. de C. Alves L.G. de Carvalho +2 位作者 E.A. Pozza L. Sanches J.C. de S. Maia 《Journal of Environmental Science and Engineering》 2011年第3期333-343,共11页
Geosciences and statistics were applied to develop predictive models to study the areas of risk to soybean rust (Phakopsora pachyrhizi Sydow) in soybean (Glycine max L.), coffee leaf rust (Hemileia vastatrix Berk... Geosciences and statistics were applied to develop predictive models to study the areas of risk to soybean rust (Phakopsora pachyrhizi Sydow) in soybean (Glycine max L.), coffee leaf rust (Hemileia vastatrix Berk & Br) in coffee and Black Sigatoka (Mycosphaerella fijiensis var. difformis) in banana, considering to Brazilian climatic characterization and distribution of soybean, coffee and banana crops in the period of observed data of 1950 to 2000 and A2 climate change scenarios of simulated data of 2020, 2050 and 2080. The technique of principal components allowed generating 1 variable based on 57 variables in order to determine an index explaining 87%, 88% and 90% of the variability of soybean, coffee and banana crops in Brazilian municipal districts. The climatic model of each disease was used to generate the zoning of the coffee rust, soybean rust and black sigatoka based on temperature and leaf wetness. Areas of favorability of the diseases were plotted inside to the main coffee, soybean and banana growing in Brazil. The applied methodology enabled to visualize the variation of favorable areas of epidemics according to the studied scenarios of climate change. 展开更多
关键词 Plant disease geographic information systems STATISTICS AGROECOSYSTEMS Brazil.
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Massive Pericardial Effusion as the First Manifestation of Hypothyroidism in a Male Patient
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作者 Livia Maria Carvalho Costa Flavia Lunardelli Negreiros de Carvalho Wagner Leao Leite Tostes 《Journal of Pharmacy and Pharmacology》 2018年第4期420-424,共5页
This article intends to report a rare case of massive pericardial effusion as the first manifestation of hypothyroidism. A 45-year-old male patient, accompanied by a cardiology department, suddenly started to present ... This article intends to report a rare case of massive pericardial effusion as the first manifestation of hypothyroidism. A 45-year-old male patient, accompanied by a cardiology department, suddenly started to present signs of dyspnea and tiredness. He was submitted to an echocardiogram and diagnosed with dilated cardiomyopathy associated with a moderate pericardial effusion and low ejection of fraction. The laboratory tests showed elevated TSH levels (13.20 mIU/L), what leads to the hypothyroidism diagnose and enable to start the treatment with levothyroxine. The patient has not followed correctly the treatment, reason why he has not showed any improves. He was admitted in the hospital to cardiology monitoring and the chest radiography confirmed an intense pericardial effusion. Then, the patient was submitted to the pericardiocentesis procedure, which was capable of remove the pericardial fluid for laboratory analysis and fragment of the pericardial sac for neoplastic cell research. After the hospital discharge, he was maintained in outpatient follow-up, when showed an important improvement in the clinical state. 展开更多
关键词 Pericardial effusion dilated cardiomyopathy HYPOTHYROIDISM THYROXINE pericardiocentesis.
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Food and Nutritional Security in the Refugee Women’s Life Narratives
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作者 Juliana Vidal Vieira Guerra Valdecyr Herdy Alves +5 位作者 Brenda Caroline Martins da Silva Tatiana do Socorro dos Santos Calandrini Giovanna Rosario Soanno Marchiori Ediane de Andrade Ferreira Maria Bertilla Lutterbach Riker Branco Diego Pereira Rodrigues 《Food and Nutrition Sciences》 2023年第11期1031-1042,共12页
Objective: To understand, based on the life narratives of refugee women, food and nutritional security. Method: Qualitative, descriptive, exploratory study, Narratives of life method, by Daniel Bertaux. Conducted with... Objective: To understand, based on the life narratives of refugee women, food and nutritional security. Method: Qualitative, descriptive, exploratory study, Narratives of life method, by Daniel Bertaux. Conducted with 11 participants, refugee women residing in the state of Rio de Janeiro, Brazil. Results: The meanings and feelings evidenced in the life narratives of refugee women expressed the insecurities and weaknesses with food in the resettlement. Final Considerations: Given the meanings and feelings regarding food in the refuge, it is expected that the present study will contribute to the elaboration of new action plans and intersectoral public policies of agile action to meet the demands of the refugee population, in the attempt to mitigate starvation, poverty, social differences and the compromised health status of the refugee population. 展开更多
关键词 Food Security Feeding Behavior Refugees Human Migration Human Right to Adequate Food
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A multiplatform metabolomic approach to characterize fecal signatures of negative postnatal events in chicks: a pilot study
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作者 Stéphane Beauclercq Antoine Lefèvre +5 位作者 Frédéric Montigny Anne Collin Sophie Tesseraud Christine Leterrier Patrick Emond Laurence A.Guilloteau 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2019年第2期424-434,共11页
Background: Negative experiences in early life can induce long-lasting effects on the welfare, health, and performance of farm animals. A delayed placement of chicks in rearing houses has negative effects on their per... Background: Negative experiences in early life can induce long-lasting effects on the welfare, health, and performance of farm animals. A delayed placement of chicks in rearing houses has negative effects on their performance, and results in fecal-specific odors detectable by rats. Based on this observation, the volatile organic compounds(VOCs) and metabolites from the feces of 12-day-old chickens were screened for early markers of response to negative events using gas-chromatography and liquid-chromatography coupled with mass spectrometry(GC-MS, LC-HRMS).Results: The low reproducibility of solid-phase micro-extraction of the VOCs followed by GC-MS was not suitable for marker discovery, in contrast to liquid extraction of metabolites from freeze-dried feces followed by GC-MS or LC-HRMS analysis. Therefore, the fecal metabolome from 12-day-old chicks having experienced a normal or delayed placement were recorded by GC-MS and LC-HRMS in two genotypes from two experiments. From both experiments, 25 and 35 metabolites, respectively explaining 81% and 45% of the difference between delayed and control chickens, were identified by orthogonal partial least-squares discriminant analysis from LC-HRMS and GC-MS profiling.Conclusion: The sets of molecules identified will be useful to better understand the chicks’ response to negative events over time and will contribute to define stress or welfare biomarkers. 展开更多
关键词 Biomarker CHICK FECES GC-MS LC-HRMS NEGATIVE POSTNATAL event
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