This research presents one possible way for imminent prediction of earthquakes’ magnitude, depth and epicenter coordinates by solving the inverse problem using a data acquisition network system for monitoring, archiv...This research presents one possible way for imminent prediction of earthquakes’ magnitude, depth and epicenter coordinates by solving the inverse problem using a data acquisition network system for monitoring, archiving and complex analysis of geophysical variables-precursors. Among many possible precursors the most reliable are the geoelectromagnetic field, the boreholes water level, radon earth-surface concentration, the local heat flow, ionosphere variables, low frequency atmosphere and Earth core waves. The title demonstrates that only geomagnetic data are used in this study. Within the framework of geomagnetic quake approach it is possible to perform an imminent regional seismic activity forecasting on the basis of simple analysis of geomagnetic data which use a new variable SChtM with dimension surface density of energy. Such analysis of Japan Memambetsu, Kakioka, Kanoya INTERMAGNET stations and NEIC earthquakes data, the hypothesis that the “predicted” earthquake is this with biggest value of the variable SChtM permits to formulate an inverse problem (overdetermined algebraic system) for precursor’s signals like a function of earthquake’s magnitude, depth and distance from a monitoring point. Thus, in the case of data acquisition network system existence, which includes monitoring of more than one reliable precursor variables in at least four points distributed within the area with a radius of up to 700 km, there will be enough algebraic equations for calculation of impending earthquake’s magnitude, depth and distance, solving the overdetermined algebraic system.展开更多
It is difficult to identify suspected cases of atypical patients with coronavirus disease 2019(COVID-19),and data on severe or critical patients are scanty.This retrospective study presents the clinical,laboratory,and...It is difficult to identify suspected cases of atypical patients with coronavirus disease 2019(COVID-19),and data on severe or critical patients are scanty.This retrospective study presents the clinical,laboratory,and radiological profiles,treatments,and outcomes of atypical COVID-19 patients without respiratory symptoms or fever at onset.The study examined ten atypical patients out of 909 severe or critical patients diagnosed with COVID-19 in Wuhan Union Hospital West Campus between 25 January 2020 and 10 February 2020.Data were obtained from the electronic medical records of severe or critical patients without respiratory symptoms or fever at onset.Outcomes were followed up to discharge or death.Among 943 COVID-19 patients,909(96.4%)were severe or critical type.Of the severe or critical patients,ten(1.1%)presented without respiratory symptoms or fever at admission.The median age of the ten participants was 63 years(interquartile range(IQR):57–72),and seven participants were men.The median time from symptom onset to admission was 14 d(IQR:7–20).Eight of the ten patients had chronic diseases.The patients had fatigue(n=5),headache or dizziness(n=4),diarrhea(n=5),anorexia(n=3),nausea or vomiting(n=3),and eye discomfort(n=1).Four patients were found to have lymphopenia.Imaging examination revealed that nine patients had bilateral pneumonia and one had unilateral pneumonia.Eventually,two patients died and eight were discharged.In the discharged patients,the median time from admission to discharge lasted 24 d(IQR:13–43).In summary,some severe or critical COVID-19 patients were found to have no respiratory symptoms or fever at onset.All such atypical cases should be identified and quarantined as early as possible,since they tend to have a prolonged hospital stay or fatal outcomes.Chest computed tomography(CT)scan and nucleic acid detection should be performed immediately on close contacts of COVID-19 patients to screen out those with atypical infections,even if the contacts present without respiratory symptoms or fever at onset.展开更多
The effects of inhibitors of TNFα converting enzyme (TACE) on TNFα secretion were studied to develop an approach to interfere inflammation processes. The HL-60 cell lines were stimulated in vitro with LPS intravenou...The effects of inhibitors of TNFα converting enzyme (TACE) on TNFα secretion were studied to develop an approach to interfere inflammation processes. The HL-60 cell lines were stimulated in vitro with LPS intravenously for different time to establish the cellular model of inflammation and simultaneously induce in vivo inflammation animal model by LPS The cytotoxic effects of soluble TNFa were checked using MTT colorimetric method to determine the rate of cell proliferation. The level of expression of TACE was detected by using RT-PCR, FCM and immuno-histochemical technique respectively. It was found Chinese medicine Reduqing (RDQ) could inhibit the transcription of TNFa mRNA induced by LPS stimulation (P<0. 01, compared with the control). The anti-oligodeoxyribonucleotide (anti-ODN) of TNFα mRNA could inhibit 78. 9 % of TNFα secretion. The mimic peptides of TACE substrates with hydroxamine group showed potency in vivo and in vitro a-gainst converting of pro-TNFα. It was concluded that all the three types of TACE inhibitors can regulate the expression of TACE at different levels and inhibit sTNFα secretion, indicating TACE is a novel target for inflammation therapy.展开更多
Conception of graphic art,is widely increased in nowadays,because of persistent acquire of new ideas and art practices,which is key feature of modern art.As a result,variety of graphic art grew heavily-constantly acqu...Conception of graphic art,is widely increased in nowadays,because of persistent acquire of new ideas and art practices,which is key feature of modern art.As a result,variety of graphic art grew heavily-constantly acquiring new aspects and experimenting with new materials,but also not forgetting about classic techniques of graphic art.That is why Latvia’s contemporary art is rich with outstanding graphics artists,which is masters of their profession.But time goes and everything sees it’s end,and on Latvia’s art horizon start to appear new faces and new talents of Latvia’s graphics art,which already had their opportunity to demonstrate highest level of their mastery and creativity,not only in Latvia,but also on international level.Living in a rhythm of modern fast-paced technology and innovation era,Latvia’s graphic artists,still say“Yes!”,to classic techniques of graphic art-linocutting,lithography and drypointing.However,they are open-minded persons and like to experiment with new techniques(for example-digiprint),or if it is suitable for their idea-combine them,to reach their goal.Aim of this paper is to get acquainted with Latvia’s next-gen graphic artists,their ideas and views,as well with results of their creative,acquiring brief review of Latvia’s graphic trends in general.As a result reader will become familiar not only with Latvia’s domestic artists and their creativity,but also will get general review of the current trends in the world of graphic art,which is not only classic,but also very innovative,regarding of new materials and techniques.展开更多
A novel glucose biosensor was constructed by electrodeposition of highly dispersed palladium (Pd) nanoparticles on a glassy carbon electrode (GCE). Atomic force microscopy (AFM) was applied to characterize its surface...A novel glucose biosensor was constructed by electrodeposition of highly dispersed palladium (Pd) nanoparticles on a glassy carbon electrode (GCE). Atomic force microscopy (AFM) was applied to characterize its surface morphology. Electrodeposited Pd nanoparticles exhibited efficiently electrocatalytic oxidation for hydrogen peroxide (H_2O_2) with relatively high sensitivity and stability,which was studied by CV technique and Raman spectroscopy,respectively. The GC/Pd/GOD/Nafion system allowed a low working potential of +0.3 V (vs. SCE). Its signal current was linearly related to the glucose concentration in the range of 1.0×10 -6 —1.2×10 -4 mol·L -1 with a detection limit of 5.0×10 -7 mol·L -1 . The sensor required no special pretreatment to suppress interference from urate and L-ascorbate. It was successfully used in detection of glucose level in human urine with high stability,sensitivity and anti-poisoning ability .展开更多
Methamphetamine is one of the most prevalent drugs abused in the world.Methamphetamine abusers usually present with hyperpyrexia (39℃),hallucination and other psychiatric symptoms.However,the detailed mechanism under...Methamphetamine is one of the most prevalent drugs abused in the world.Methamphetamine abusers usually present with hyperpyrexia (39℃),hallucination and other psychiatric symptoms.However,the detailed mechanism underlying its neurotoxic action remains elusive.This study investigated the effects of methamphetamine + 39℃ on primary cortical neurons from the cortex of embryonic Sprague-Dawley rats.Primary cortex neurons were exposed to 1 mM methamphetamine + 39℃.Propidium iodide staining and lactate dehydrogenase release detection showed that methamphetamine + 39℃ triggered obvious necrosis-like death in cultured primary cortical neurons,which could be partially inhibited by receptor-interacting protein-1 (RIP1) inhibitor Necrostatin-1 partially.Western blot assay results showed that there were increases in the expressions of receptor-interacting protein-3 (RIP3) and mixed lineage kinase domain-like protein (MLKL) in the primary cortical neurons treated with 1 mM methamphetamine + 39℃ for 3 hours.After pre-treatment with RIP3 inhibitor GSK’872,propidium iodide staining and lactate dehydrogenase release detection showed that neuronal necrosis rate was significantly decreased;RIP3 and MLKL protein expression significantly decreased.Immunohistochemistry staining results also showed that the expressions of RIP3 and MLKL were up-regulated in brain specimens from humans who had died of methamphetamine abuse.Taken together,the above results suggest that methamphetamine + 39℃ can induce RIP3/MLKL regulated necroptosis,thereby resulting in neurotoxicity.The study protocol was approved by the Medical Ethics Committee of the Third Xiangya Hospital of Central South University,China (approval numbers: 2017-S026 and 2017-S033) on March 7,2017.展开更多
Plants in natural ecosystems are exposed to a combination of UV radiation,ionizing radiation(IR)and other abiotic factors.These factors change with the altitude.We investigated DNA alterations of some wild plants of d...Plants in natural ecosystems are exposed to a combination of UV radiation,ionizing radiation(IR)and other abiotic factors.These factors change with the altitude.We investigated DNA alterations of some wild plants of different plant families in natural ecosystems at three altitudes in Rila Mountain,Bulgaria(1500,1782,and 2925 m above sea level(a.s.l.)exposed to UV radiation,IR and other abiotic stresses,to assess the tolerance of plant species to the changing environmental conditions in three successive growth seasons.For this purpose,physicochemical,cytogenetic,and molecular methods were applied.DNA damage was assessed by micronucleus test and molecular method comet assay adapted and applied by us to wild plant species from Onagraceae,Rosaceae,Boraginaceae,Saxifragaceae,Orobanchaceae,Asteraceae and Poaceae families,growing at three different altitudes.Variability in the DNA sensitivity and the level of tolerance was observed among the plant species in response to combined abiotic factors assessed by induced DNA damage and gross beta activity.The studied representatives of Poaceae were less susceptible than the other studied species at all three altitudes and showed close level of DNA injuries to that of unaffected control plant grown in laboratory conditions.The lower levels of DNA damage of these wild plant species corresponded to their lower ability to accumulate radionuclides.There was a particularly pronounced low level of DNA injuries in the plant species at the highest altitude.The level of DNA damage showed correlation with the values of some abiotic environmental factors.The results would contribute to the elucidation of the extent of adaptation of plant species to the continuously changing environment and would be useful in selecting sensitive herbaceous monitor species for environmental impact assessment at mountain and alpine sites.展开更多
The relationship between the levels of renalase and changes in proteinuria, hypertension, renal function, renal tubular epithelial cell apoptosis and B-cell lymphoma-2 （Bcl-2） expression was investigated in patients...The relationship between the levels of renalase and changes in proteinuria, hypertension, renal function, renal tubular epithelial cell apoptosis and B-cell lymphoma-2 （Bcl-2） expression was investigated in patients （chronic nephritis, primary nephrotic syndrome or other kidney disease） that underwent renal biopsy. The study group comprised 72 patients undergoing renal biopsy. Patient profiles and renal function were collected. Concentrations of renalase and Bcl-2 were measured by immunohistochemistry. Tubular injury was detected by periodic acid Schiff staining （PAS） and renal tubular epithelial cell apoptosis was assessed by TUNEL assay. The expression of renalase was significantly lower in renal biopsy specimens than in normal kidney tissues. There was a positive linear relationship between renalase and some serum and cardiac indices; a negative correlation was found between age, eGFR, Ccr and 24-h urinary protein. Renal tubule injury index and tubular epithelial cell apoptosis index showed a negative linear correlation with renalase. The results showed that renalase probably increased the expression of Bel-2. By two independent samples t-test, renalase levels were significantly increased in the non-hypertension group than in the hypertension group. One-way ANOVA showed that renalase expression was higher in samples with Lee＇s grade Ⅲ than in those with Lee＇s grade V. The expression of renalase was significantly decreased in patients who underwent renal biopsy, and was also associated with blood and renal function. The research proved that renalase may reduce renal tubular injury and apoptosis of renal tubular epithelial cells through the mitochondrial apoptosis pathway, finally achieving the purpose of delaying the progress of renal failure.展开更多
This article examines some general atmospheric circulation and climate models in the context of the notion of “memory”. Two kinds of memories are defined： statistical memory and deterministic memory. The former is ...This article examines some general atmospheric circulation and climate models in the context of the notion of “memory”. Two kinds of memories are defined： statistical memory and deterministic memory. The former is defined through the autocorrelation characteristic of the process if it is random （chaotic）, while for the latter, a special memory function is introduced. Three of the numerous existing models are selected as examples. For each of the models, asymptotic （at t →∞） expressions are derived. In this way, the transients are filtered out and that which remains concerns the final behaviour of the models.展开更多
It is already well known that the “when, where and how strong” earthquake prediction problem cannot be solved by only analyzing the database from former earthquakes. A possible solution to this problem is proposed h...It is already well known that the “when, where and how strong” earthquake prediction problem cannot be solved by only analyzing the database from former earthquakes. A possible solution to this problem is proposed herein based on the analysis of the physicochemical processes as participants in earthquake preparation and on the characteristic rate of reflection of these processes on the Earth’s surface. The proposed procedure includes monitoring of correlation of electromagnetic fields variations with tidal waves.?This solution provides a way of selecting a complex of reliable earthquake precursors using the Inverse Problem Method for earthquakes which will occur in the region around the monitoring point (radial distance ≈ 700 km) in the next seven-day period [1].展开更多
In this paper,an irregular displacement-based lensless wide-field microscopy imaging platform is presented by combining digital in-line holography and computational pixel super-resolution using multi-frame processing....In this paper,an irregular displacement-based lensless wide-field microscopy imaging platform is presented by combining digital in-line holography and computational pixel super-resolution using multi-frame processing.The samples are illuminated by a nearly coherent illumination system,where the hologram shadows are projected into a complementary metal-oxide semiconductor-based imaging sensor.To increase the resolution,a multi-frame pixel resolution approach is employed to produce a single holographic image from multiple frame observations of the scene,with small planar displacements.Displacements are resolved by a hybrid approach:(i)alignment of the LR images by a fast feature-based registration method,and(ii)fine adjustment of the sub-pixel information using a continuous optimization approach designed to find the global optimum solution.Numerical method for phase-retrieval is applied to decode the signal and reconstruct the morphological details of the analyzed sample.The presented approach was evaluated with various biological samples including sperm and platelets,whose dimensions are in the order of a few microns.The obtained results demonstrate a spatial resolution of 1.55 μm on a field-of-view of<30 mm^(2).展开更多
The aim of this paper is the introduction of a new approach to 3D modelling of elastic piecewise homogeneous media, in particular Earth crust and upper Mantle. The method is based on the principle of tomography with E...The aim of this paper is the introduction of a new approach to 3D modelling of elastic piecewise homogeneous media, in particular Earth crust and upper Mantle. The method is based on the principle of tomography with Earthquake as a source of the signal and receiver stations on the surface. The wave propagation in solid media is described by a system of three strongly coupled hyperbolic equations with piece - wise constant coefitients. The characteristic set and hi-characteristic curves of this system are computed in a homogeneous half-space with free boundary and the formulae of reflection and diffraction of the hi-characteristics on the internal boundaries of the media. Applications of the characteristic set and bi-eharacteristic curves for the inverse problem in geophysics and Earth modelling are given.展开更多
Introduction: Antenatal care (ANC) contributes to the reduction of maternal and neonatal morbidity and mortality. The study aimed to investigate the determinants of the low proportion of pregnant women seen for first ...Introduction: Antenatal care (ANC) contributes to the reduction of maternal and neonatal morbidity and mortality. The study aimed to investigate the determinants of the low proportion of pregnant women seen for first antenatal care in the first trimester of pregnancy at the urban medical center of Koudougou, Burkina Faso. Materials and Methods: This was a cross-sectional study with data collection from June 08 to August 18, 2021. It involved a sample of 302 participants including 280 pregnant women and 22 maternity providers. Semi-structured individual interviews, non-participant observation, and a literature review were used. Results: Pregnant women age 20 and over, knowledge of the date of their last menstrual period, and knowledge of the antenatal care calendar were associated with coming into contact (1) with Antenatal care in the first trimester of pregnancy. In addition, there was a lack of availability of antenatal care services, inadequate reception of clients, and shortages of health products. Conclusion: There is a need to revisit strategies for communicating with women about ANC, reorganizing ANC services, and improving ANC services.展开更多
High-spin states in 69As were populated via the 40Ca(32S, 3p)69As reaction at 95 MeV beam energy. The 32S beam was provided by the VIVITRON accelerator of the IreS Strasbourg. A target of 1 mg/cm2 With 15 mg/cm2 gold ...High-spin states in 69As were populated via the 40Ca(32S, 3p)69As reaction at 95 MeV beam energy. The 32S beam was provided by the VIVITRON accelerator of the IreS Strasbourg. A target of 1 mg/cm2 With 15 mg/cm2 gold backing was used. Measurements of γ-γand γ-γ-γcoincidences were performed with the EUROBALL展开更多
The occurrence of seven endocrine-disrupting compounds(EDCs)in four urban rivers on the southern border of Mexico was studied in this work.The selected EDCs were 17b-estradiol(E2),estriol(E3),17aethinylestradiol(EE2),...The occurrence of seven endocrine-disrupting compounds(EDCs)in four urban rivers on the southern border of Mexico was studied in this work.The selected EDCs were 17b-estradiol(E2),estriol(E3),17aethinylestradiol(EE2),bisphenol A(BPA),bisphenol F(BPF),4-nonylphenol(4NP)and 4-tert-octylphenol(4TOP).Water samples from the Coatan,Texcuyuapan,Cahoacan,and Coatancito rivers were collected at three different sites on each river and processed by solid phase extraction(SPE).The samples were analyzed by gas chromatography triple quadrupole tandem mass spectrometry(GC-MS/MS)and compounds of interest were identified by dynamic mass reaction monitoring mode(dMRM).Among the target EDCs,E2,BPA,and 4NP were detected in all water samples,with BPA being the most abundant ranging from 4.22 up to 127.96 ng/L.Correlation tests showed significant positive associations between estrogens and BPA,as well as the correlation between alkylphenols and BPA,suggesting common origin emission sources,which were identified as discharges of untreated municipal wastewater.Finally,the estimation of RQ and EEQ values indicated that the presence of E2,E3,EE2,and BPA in all sampled points represents a high risk for aquatic life in these sites,mainly for Texcuyuapan River,where the aquatic organisms may be susceptible to endocrine disruption by chronic exposure to detected compounds.展开更多
Dear Editor,The Hippo tumor suppressor pathway plays an important role in development and tumorigenesis.Yes-associated protein(YAP)is a major effector of the Hippo pathway regulating the expression of genes involved i...Dear Editor,The Hippo tumor suppressor pathway plays an important role in development and tumorigenesis.Yes-associated protein(YAP)is a major effector of the Hippo pathway regulating the expression of genes involved in cell proliferation,cell death,and cell differentiation(Pan,2010;Johnson and Haider,2014;Piccolo etal.,2014;Yu etal.,2015).In intestine,YAP is mainly expressed in leucine rich repeat containing G protein-coupled receptor 5(LGR5)marked stem cells,and is required for regeneration following tissue damage and the development of colon cancer in mice(Cai etal.,2010;Barry etal.,2013;Wang etal.,2017).Moreover,in colorectal cancer(CRC)specimens,elevated YAP expression and activity is frequently observed(Steinhardt et al.,2008).It has been shown that high YAP expression is associated with cancer metastasis,poor prognosis,resistance to chemotherapy,and greater possibility of relapse(Johnson and Haider,2014;Yu etal.,2015).展开更多
Solid-state batteries(SSBs)are a promising next step in electrochemical energy storage but are plagued by a number of problems.In this study,we demonstrate the recurring issue of mechanical degradation because of volu...Solid-state batteries(SSBs)are a promising next step in electrochemical energy storage but are plagued by a number of problems.In this study,we demonstrate the recurring issue of mechanical degradation because of volume changes in layered Ni-rich oxide cathode materials in thiophosphate-based SSBs.Specifically,we explore superionic solid electrolytes(SEs)of different crystallinity,namely glassy 1.5Li_(2)S-0.5P_(2)S_(5)-LiI and argyrodite Li_(6)PS_(5)Cl,with emphasis on how they affect the cyclability of slurry-cast cathodes with NCM622(60%Ni)or NCM851005(85%Ni).The application of a combination of ex situ and in situ analytical techniques helped to reveal the benefits of using a SE with a low Young’s modulus.Through a synergistic interplay of(electro)chemical and(chemo)mechanical effects,the glassy SE employed in this work was able to achieve robust and stable interfaces,enabling intimate contact with the cathode material while at the same time mitigating volume changes.Our results emphasize the importance of considering chemical,electrochemical,and mechanical properties to realize long-term cycling performance in high-loading SSBs.展开更多
文摘This research presents one possible way for imminent prediction of earthquakes’ magnitude, depth and epicenter coordinates by solving the inverse problem using a data acquisition network system for monitoring, archiving and complex analysis of geophysical variables-precursors. Among many possible precursors the most reliable are the geoelectromagnetic field, the boreholes water level, radon earth-surface concentration, the local heat flow, ionosphere variables, low frequency atmosphere and Earth core waves. The title demonstrates that only geomagnetic data are used in this study. Within the framework of geomagnetic quake approach it is possible to perform an imminent regional seismic activity forecasting on the basis of simple analysis of geomagnetic data which use a new variable SChtM with dimension surface density of energy. Such analysis of Japan Memambetsu, Kakioka, Kanoya INTERMAGNET stations and NEIC earthquakes data, the hypothesis that the “predicted” earthquake is this with biggest value of the variable SChtM permits to formulate an inverse problem (overdetermined algebraic system) for precursor’s signals like a function of earthquake’s magnitude, depth and distance from a monitoring point. Thus, in the case of data acquisition network system existence, which includes monitoring of more than one reliable precursor variables in at least four points distributed within the area with a radius of up to 700 km, there will be enough algebraic equations for calculation of impending earthquake’s magnitude, depth and distance, solving the overdetermined algebraic system.
基金This research was supported by the National Natural Science Foundation of China(82041018,81770096,81700091,and 81800094)the National Science and Technology Major Project of the Ministry of Science and Technology of China(2019ZX09301001)+1 种基金the National Key Research and Development Program of the Ministry of Science and Technology of China(2020YFC0844300)the Fundamental Research Funds for the Central Universities(China)(2020kfyXGYJ011).
文摘It is difficult to identify suspected cases of atypical patients with coronavirus disease 2019(COVID-19),and data on severe or critical patients are scanty.This retrospective study presents the clinical,laboratory,and radiological profiles,treatments,and outcomes of atypical COVID-19 patients without respiratory symptoms or fever at onset.The study examined ten atypical patients out of 909 severe or critical patients diagnosed with COVID-19 in Wuhan Union Hospital West Campus between 25 January 2020 and 10 February 2020.Data were obtained from the electronic medical records of severe or critical patients without respiratory symptoms or fever at onset.Outcomes were followed up to discharge or death.Among 943 COVID-19 patients,909(96.4%)were severe or critical type.Of the severe or critical patients,ten(1.1%)presented without respiratory symptoms or fever at admission.The median age of the ten participants was 63 years(interquartile range(IQR):57–72),and seven participants were men.The median time from symptom onset to admission was 14 d(IQR:7–20).Eight of the ten patients had chronic diseases.The patients had fatigue(n=5),headache or dizziness(n=4),diarrhea(n=5),anorexia(n=3),nausea or vomiting(n=3),and eye discomfort(n=1).Four patients were found to have lymphopenia.Imaging examination revealed that nine patients had bilateral pneumonia and one had unilateral pneumonia.Eventually,two patients died and eight were discharged.In the discharged patients,the median time from admission to discharge lasted 24 d(IQR:13–43).In summary,some severe or critical COVID-19 patients were found to have no respiratory symptoms or fever at onset.All such atypical cases should be identified and quarantined as early as possible,since they tend to have a prolonged hospital stay or fatal outcomes.Chest computed tomography(CT)scan and nucleic acid detection should be performed immediately on close contacts of COVID-19 patients to screen out those with atypical infections,even if the contacts present without respiratory symptoms or fever at onset.
基金This project was supported by a grant from National Natural Sciences Foundation of China(No.30070722).
文摘The effects of inhibitors of TNFα converting enzyme (TACE) on TNFα secretion were studied to develop an approach to interfere inflammation processes. The HL-60 cell lines were stimulated in vitro with LPS intravenously for different time to establish the cellular model of inflammation and simultaneously induce in vivo inflammation animal model by LPS The cytotoxic effects of soluble TNFa were checked using MTT colorimetric method to determine the rate of cell proliferation. The level of expression of TACE was detected by using RT-PCR, FCM and immuno-histochemical technique respectively. It was found Chinese medicine Reduqing (RDQ) could inhibit the transcription of TNFa mRNA induced by LPS stimulation (P<0. 01, compared with the control). The anti-oligodeoxyribonucleotide (anti-ODN) of TNFα mRNA could inhibit 78. 9 % of TNFα secretion. The mimic peptides of TACE substrates with hydroxamine group showed potency in vivo and in vitro a-gainst converting of pro-TNFα. It was concluded that all the three types of TACE inhibitors can regulate the expression of TACE at different levels and inhibit sTNFα secretion, indicating TACE is a novel target for inflammation therapy.
文摘Conception of graphic art,is widely increased in nowadays,because of persistent acquire of new ideas and art practices,which is key feature of modern art.As a result,variety of graphic art grew heavily-constantly acquiring new aspects and experimenting with new materials,but also not forgetting about classic techniques of graphic art.That is why Latvia’s contemporary art is rich with outstanding graphics artists,which is masters of their profession.But time goes and everything sees it’s end,and on Latvia’s art horizon start to appear new faces and new talents of Latvia’s graphics art,which already had their opportunity to demonstrate highest level of their mastery and creativity,not only in Latvia,but also on international level.Living in a rhythm of modern fast-paced technology and innovation era,Latvia’s graphic artists,still say“Yes!”,to classic techniques of graphic art-linocutting,lithography and drypointing.However,they are open-minded persons and like to experiment with new techniques(for example-digiprint),or if it is suitable for their idea-combine them,to reach their goal.Aim of this paper is to get acquainted with Latvia’s next-gen graphic artists,their ideas and views,as well with results of their creative,acquiring brief review of Latvia’s graphic trends in general.As a result reader will become familiar not only with Latvia’s domestic artists and their creativity,but also will get general review of the current trends in the world of graphic art,which is not only classic,but also very innovative,regarding of new materials and techniques.
基金ProjectsupportedbytheNaturalScienceFoundationofChina (No .2 0 175 0 0 6)andtheSpecialFundationforNanoTechnologyofShanghai (No .0 2 14nm0 78andNo .0 114nm 0 72 )
文摘A novel glucose biosensor was constructed by electrodeposition of highly dispersed palladium (Pd) nanoparticles on a glassy carbon electrode (GCE). Atomic force microscopy (AFM) was applied to characterize its surface morphology. Electrodeposited Pd nanoparticles exhibited efficiently electrocatalytic oxidation for hydrogen peroxide (H_2O_2) with relatively high sensitivity and stability,which was studied by CV technique and Raman spectroscopy,respectively. The GC/Pd/GOD/Nafion system allowed a low working potential of +0.3 V (vs. SCE). Its signal current was linearly related to the glucose concentration in the range of 1.0×10 -6 —1.2×10 -4 mol·L -1 with a detection limit of 5.0×10 -7 mol·L -1 . The sensor required no special pretreatment to suppress interference from urate and L-ascorbate. It was successfully used in detection of glucose level in human urine with high stability,sensitivity and anti-poisoning ability .
基金funded by the National Natural Science Foundation of China,No.81971891(to KX),81571939(to KX),81772134(to KX),81772024(to JY),and 81860781(to FXL)the Key Research and Development Program of Hunan Province of China,No.2018SK2091(to KX)+1 种基金the Natural Science Foundation of Hunan Province of China,No.2017JJ2339(to JY)the Wu Jie-Ping Medical Foundation of the Minister of Health of China,No.320.6750.14118(to KX)
文摘Methamphetamine is one of the most prevalent drugs abused in the world.Methamphetamine abusers usually present with hyperpyrexia (39℃),hallucination and other psychiatric symptoms.However,the detailed mechanism underlying its neurotoxic action remains elusive.This study investigated the effects of methamphetamine + 39℃ on primary cortical neurons from the cortex of embryonic Sprague-Dawley rats.Primary cortex neurons were exposed to 1 mM methamphetamine + 39℃.Propidium iodide staining and lactate dehydrogenase release detection showed that methamphetamine + 39℃ triggered obvious necrosis-like death in cultured primary cortical neurons,which could be partially inhibited by receptor-interacting protein-1 (RIP1) inhibitor Necrostatin-1 partially.Western blot assay results showed that there were increases in the expressions of receptor-interacting protein-3 (RIP3) and mixed lineage kinase domain-like protein (MLKL) in the primary cortical neurons treated with 1 mM methamphetamine + 39℃ for 3 hours.After pre-treatment with RIP3 inhibitor GSK’872,propidium iodide staining and lactate dehydrogenase release detection showed that neuronal necrosis rate was significantly decreased;RIP3 and MLKL protein expression significantly decreased.Immunohistochemistry staining results also showed that the expressions of RIP3 and MLKL were up-regulated in brain specimens from humans who had died of methamphetamine abuse.Taken together,the above results suggest that methamphetamine + 39℃ can induce RIP3/MLKL regulated necroptosis,thereby resulting in neurotoxicity.The study protocol was approved by the Medical Ethics Committee of the Third Xiangya Hospital of Central South University,China (approval numbers: 2017-S026 and 2017-S033) on March 7,2017.
基金This work was supported by a grant of the National Science Fund of the Republic of Bulgaria under Project No.DN 04/1,13.12.2016 entitled:“Study of the combined effect of the natural radioactivity background,the UV radiation,the climate changes and the cosmic rays on model groups of plant and animal organisms in mountain ecosystems”.
文摘Plants in natural ecosystems are exposed to a combination of UV radiation,ionizing radiation(IR)and other abiotic factors.These factors change with the altitude.We investigated DNA alterations of some wild plants of different plant families in natural ecosystems at three altitudes in Rila Mountain,Bulgaria(1500,1782,and 2925 m above sea level(a.s.l.)exposed to UV radiation,IR and other abiotic stresses,to assess the tolerance of plant species to the changing environmental conditions in three successive growth seasons.For this purpose,physicochemical,cytogenetic,and molecular methods were applied.DNA damage was assessed by micronucleus test and molecular method comet assay adapted and applied by us to wild plant species from Onagraceae,Rosaceae,Boraginaceae,Saxifragaceae,Orobanchaceae,Asteraceae and Poaceae families,growing at three different altitudes.Variability in the DNA sensitivity and the level of tolerance was observed among the plant species in response to combined abiotic factors assessed by induced DNA damage and gross beta activity.The studied representatives of Poaceae were less susceptible than the other studied species at all three altitudes and showed close level of DNA injuries to that of unaffected control plant grown in laboratory conditions.The lower levels of DNA damage of these wild plant species corresponded to their lower ability to accumulate radionuclides.There was a particularly pronounced low level of DNA injuries in the plant species at the highest altitude.The level of DNA damage showed correlation with the values of some abiotic environmental factors.The results would contribute to the elucidation of the extent of adaptation of plant species to the continuously changing environment and would be useful in selecting sensitive herbaceous monitor species for environmental impact assessment at mountain and alpine sites.
文摘The relationship between the levels of renalase and changes in proteinuria, hypertension, renal function, renal tubular epithelial cell apoptosis and B-cell lymphoma-2 （Bcl-2） expression was investigated in patients （chronic nephritis, primary nephrotic syndrome or other kidney disease） that underwent renal biopsy. The study group comprised 72 patients undergoing renal biopsy. Patient profiles and renal function were collected. Concentrations of renalase and Bcl-2 were measured by immunohistochemistry. Tubular injury was detected by periodic acid Schiff staining （PAS） and renal tubular epithelial cell apoptosis was assessed by TUNEL assay. The expression of renalase was significantly lower in renal biopsy specimens than in normal kidney tissues. There was a positive linear relationship between renalase and some serum and cardiac indices; a negative correlation was found between age, eGFR, Ccr and 24-h urinary protein. Renal tubule injury index and tubular epithelial cell apoptosis index showed a negative linear correlation with renalase. The results showed that renalase probably increased the expression of Bel-2. By two independent samples t-test, renalase levels were significantly increased in the non-hypertension group than in the hypertension group. One-way ANOVA showed that renalase expression was higher in samples with Lee＇s grade Ⅲ than in those with Lee＇s grade V. The expression of renalase was significantly decreased in patients who underwent renal biopsy, and was also associated with blood and renal function. The research proved that renalase may reduce renal tubular injury and apoptosis of renal tubular epithelial cells through the mitochondrial apoptosis pathway, finally achieving the purpose of delaying the progress of renal failure.
文摘This article examines some general atmospheric circulation and climate models in the context of the notion of “memory”. Two kinds of memories are defined： statistical memory and deterministic memory. The former is defined through the autocorrelation characteristic of the process if it is random （chaotic）, while for the latter, a special memory function is introduced. Three of the numerous existing models are selected as examples. For each of the models, asymptotic （at t →∞） expressions are derived. In this way, the transients are filtered out and that which remains concerns the final behaviour of the models.
文摘It is already well known that the “when, where and how strong” earthquake prediction problem cannot be solved by only analyzing the database from former earthquakes. A possible solution to this problem is proposed herein based on the analysis of the physicochemical processes as participants in earthquake preparation and on the characteristic rate of reflection of these processes on the Earth’s surface. The proposed procedure includes monitoring of correlation of electromagnetic fields variations with tidal waves.?This solution provides a way of selecting a complex of reliable earthquake precursors using the Inverse Problem Method for earthquakes which will occur in the region around the monitoring point (radial distance ≈ 700 km) in the next seven-day period [1].
基金We would like to acknowledge National Institute of Health(NIH)NIH R01AI093282,NIH R01AI081534,NIH U54EB15408,NIH R21AI087107,and Brigham and Women’s Hospital-BRI Translatable Technologies and Care Innovation GrantWe would also like to acknowledge the support provided by the Brazilian National Council for Scientific and Technological Development(process 551967/2011-0)
文摘In this paper,an irregular displacement-based lensless wide-field microscopy imaging platform is presented by combining digital in-line holography and computational pixel super-resolution using multi-frame processing.The samples are illuminated by a nearly coherent illumination system,where the hologram shadows are projected into a complementary metal-oxide semiconductor-based imaging sensor.To increase the resolution,a multi-frame pixel resolution approach is employed to produce a single holographic image from multiple frame observations of the scene,with small planar displacements.Displacements are resolved by a hybrid approach:(i)alignment of the LR images by a fast feature-based registration method,and(ii)fine adjustment of the sub-pixel information using a continuous optimization approach designed to find the global optimum solution.Numerical method for phase-retrieval is applied to decode the signal and reconstruct the morphological details of the analyzed sample.The presented approach was evaluated with various biological samples including sperm and platelets,whose dimensions are in the order of a few microns.The obtained results demonstrate a spatial resolution of 1.55 μm on a field-of-view of<30 mm^(2).
文摘The aim of this paper is the introduction of a new approach to 3D modelling of elastic piecewise homogeneous media, in particular Earth crust and upper Mantle. The method is based on the principle of tomography with Earthquake as a source of the signal and receiver stations on the surface. The wave propagation in solid media is described by a system of three strongly coupled hyperbolic equations with piece - wise constant coefitients. The characteristic set and hi-characteristic curves of this system are computed in a homogeneous half-space with free boundary and the formulae of reflection and diffraction of the hi-characteristics on the internal boundaries of the media. Applications of the characteristic set and bi-eharacteristic curves for the inverse problem in geophysics and Earth modelling are given.
文摘Introduction: Antenatal care (ANC) contributes to the reduction of maternal and neonatal morbidity and mortality. The study aimed to investigate the determinants of the low proportion of pregnant women seen for first antenatal care in the first trimester of pregnancy at the urban medical center of Koudougou, Burkina Faso. Materials and Methods: This was a cross-sectional study with data collection from June 08 to August 18, 2021. It involved a sample of 302 participants including 280 pregnant women and 22 maternity providers. Semi-structured individual interviews, non-participant observation, and a literature review were used. Results: Pregnant women age 20 and over, knowledge of the date of their last menstrual period, and knowledge of the antenatal care calendar were associated with coming into contact (1) with Antenatal care in the first trimester of pregnancy. In addition, there was a lack of availability of antenatal care services, inadequate reception of clients, and shortages of health products. Conclusion: There is a need to revisit strategies for communicating with women about ANC, reorganizing ANC services, and improving ANC services.
文摘High-spin states in 69As were populated via the 40Ca(32S, 3p)69As reaction at 95 MeV beam energy. The 32S beam was provided by the VIVITRON accelerator of the IreS Strasbourg. A target of 1 mg/cm2 With 15 mg/cm2 gold backing was used. Measurements of γ-γand γ-γ-γcoincidences were performed with the EUROBALL
基金the Universidad Politecnica de Tapachula(UPTap)and Centro de Investigaciones Químicas,Universidad Autonoma del Estado de Morelos for all facilities granted to carry out this work.The first author Lopez-Velazquez(CVU 736037)thanks the Consejo Nacional de HumanidadesCiencias y Tecnologías(CONAHCYT)and the program Estancias Posdoctorales por Mexico-Continuidad 2022(1)for the funding granted.We also thank Kevin G.Duque Olivera for his support during the sampling seasons.
文摘The occurrence of seven endocrine-disrupting compounds(EDCs)in four urban rivers on the southern border of Mexico was studied in this work.The selected EDCs were 17b-estradiol(E2),estriol(E3),17aethinylestradiol(EE2),bisphenol A(BPA),bisphenol F(BPF),4-nonylphenol(4NP)and 4-tert-octylphenol(4TOP).Water samples from the Coatan,Texcuyuapan,Cahoacan,and Coatancito rivers were collected at three different sites on each river and processed by solid phase extraction(SPE).The samples were analyzed by gas chromatography triple quadrupole tandem mass spectrometry(GC-MS/MS)and compounds of interest were identified by dynamic mass reaction monitoring mode(dMRM).Among the target EDCs,E2,BPA,and 4NP were detected in all water samples,with BPA being the most abundant ranging from 4.22 up to 127.96 ng/L.Correlation tests showed significant positive associations between estrogens and BPA,as well as the correlation between alkylphenols and BPA,suggesting common origin emission sources,which were identified as discharges of untreated municipal wastewater.Finally,the estimation of RQ and EEQ values indicated that the presence of E2,E3,EE2,and BPA in all sampled points represents a high risk for aquatic life in these sites,mainly for Texcuyuapan River,where the aquatic organisms may be susceptible to endocrine disruption by chronic exposure to detected compounds.
基金This work was supported by Grants from the National Natural Science Foundation of China(Grant Nos.31571479,81622038,81772965,31830107,31821002,and 31425008)the National Key R&D Program of China(2016YFA0500700 and 2018YFA0800304)+3 种基金Commission of Shanghai Municipality(19JC1411100 and 2017BR018)the National Ten-Thousand Talent Program(Y.X.)the National Program for support of Top-Notch Young Professionals(Y.X.)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB08000000).
文摘Dear Editor,The Hippo tumor suppressor pathway plays an important role in development and tumorigenesis.Yes-associated protein(YAP)is a major effector of the Hippo pathway regulating the expression of genes involved in cell proliferation,cell death,and cell differentiation(Pan,2010;Johnson and Haider,2014;Piccolo etal.,2014;Yu etal.,2015).In intestine,YAP is mainly expressed in leucine rich repeat containing G protein-coupled receptor 5(LGR5)marked stem cells,and is required for regeneration following tissue damage and the development of colon cancer in mice(Cai etal.,2010;Barry etal.,2013;Wang etal.,2017).Moreover,in colorectal cancer(CRC)specimens,elevated YAP expression and activity is frequently observed(Steinhardt et al.,2008).It has been shown that high YAP expression is associated with cancer metastasis,poor prognosis,resistance to chemotherapy,and greater possibility of relapse(Johnson and Haider,2014;Yu etal.,2015).
基金This study was supported by BASF SE.F Strauss acknowledges financial support from the Fonds der Chemischen Industrie through a Liebig fellowship.
文摘Solid-state batteries(SSBs)are a promising next step in electrochemical energy storage but are plagued by a number of problems.In this study,we demonstrate the recurring issue of mechanical degradation because of volume changes in layered Ni-rich oxide cathode materials in thiophosphate-based SSBs.Specifically,we explore superionic solid electrolytes(SEs)of different crystallinity,namely glassy 1.5Li_(2)S-0.5P_(2)S_(5)-LiI and argyrodite Li_(6)PS_(5)Cl,with emphasis on how they affect the cyclability of slurry-cast cathodes with NCM622(60%Ni)or NCM851005(85%Ni).The application of a combination of ex situ and in situ analytical techniques helped to reveal the benefits of using a SE with a low Young’s modulus.Through a synergistic interplay of(electro)chemical and(chemo)mechanical effects,the glassy SE employed in this work was able to achieve robust and stable interfaces,enabling intimate contact with the cathode material while at the same time mitigating volume changes.Our results emphasize the importance of considering chemical,electrochemical,and mechanical properties to realize long-term cycling performance in high-loading SSBs.
