Objective:To isolate and culture PSSCs from different tissues and determine their characteristics and differentiation potential in vitro. Methods: PSSCs were isolated and cultured from human aborted fetal bone marrow,...Objective:To isolate and culture PSSCs from different tissues and determine their characteristics and differentiation potential in vitro. Methods: PSSCs were isolated and cultured from human aborted fetal bone marrow, liver, skin, skeletal muscle, lung and pancreas. Morphology and biological activities were assessed. Phenotypes were analyzed by FACS and immunohistochemical staining. We tested the potential of PSSCs to differentiate into multiple cell lineages, such as bone, cartilage, fat, muscle, nerve , endothelial cell and hematopoietic progenitor cells. Results:PSSCs could be isolated from human aborted fetal above. PSSCs were a population of adherent cells characterized by a typical fibroblast-like morphology. PSSCs had few endoplasmic reticulum and mitochondrias. It could be expanded by successive cycles of trypsinization, seeding, and culture ex vitro. PSSCs had a capability of passaging up to 30 times without displaying significant changes in morphology, with a 2-fold increase in cell number after each passage. Cell cycle analysis revealed that more than 90% of cells were in the G0/G1 phases, while a small population of cells were actively engaged in proliferation. These cells were positively stained by FITC labeled CD44, CD29, CD13, but negative for CD34, HLA-DR. The culture-expanded PSSCs have multilineage differentiation potential giving rise to cells of osteogenic, chondrogenic, adipogenic, myogenic, neurogenic, hematopoietic and endothelial lineages. Conclusion:PSSCs may still remain in a number of tissues after embryonic development,could be identified by their phenotypic and functional characteristics, and contribute significantly to multipotent differentiation outside the tissue of origin.展开更多
文摘Objective:To isolate and culture PSSCs from different tissues and determine their characteristics and differentiation potential in vitro. Methods: PSSCs were isolated and cultured from human aborted fetal bone marrow, liver, skin, skeletal muscle, lung and pancreas. Morphology and biological activities were assessed. Phenotypes were analyzed by FACS and immunohistochemical staining. We tested the potential of PSSCs to differentiate into multiple cell lineages, such as bone, cartilage, fat, muscle, nerve , endothelial cell and hematopoietic progenitor cells. Results:PSSCs could be isolated from human aborted fetal above. PSSCs were a population of adherent cells characterized by a typical fibroblast-like morphology. PSSCs had few endoplasmic reticulum and mitochondrias. It could be expanded by successive cycles of trypsinization, seeding, and culture ex vitro. PSSCs had a capability of passaging up to 30 times without displaying significant changes in morphology, with a 2-fold increase in cell number after each passage. Cell cycle analysis revealed that more than 90% of cells were in the G0/G1 phases, while a small population of cells were actively engaged in proliferation. These cells were positively stained by FITC labeled CD44, CD29, CD13, but negative for CD34, HLA-DR. The culture-expanded PSSCs have multilineage differentiation potential giving rise to cells of osteogenic, chondrogenic, adipogenic, myogenic, neurogenic, hematopoietic and endothelial lineages. Conclusion:PSSCs may still remain in a number of tissues after embryonic development,could be identified by their phenotypic and functional characteristics, and contribute significantly to multipotent differentiation outside the tissue of origin.
