Background:Rhizoma Dioscoreae bulbiferae could cause liver damage,which limited its application in the clinic.Aims and Objectives:To explore the mechanism of hepatotoxicity of Rhizoma Dioscoreae bulbiferae in mice.Mat...Background:Rhizoma Dioscoreae bulbiferae could cause liver damage,which limited its application in the clinic.Aims and Objectives:To explore the mechanism of hepatotoxicity of Rhizoma Dioscoreae bulbiferae in mice.Materials and Methods:In the present study,the water extraction of Rhizoma Dioscoreae bulbiferae(W.E.R)was administrated via intragastrical with Low(19.6 g/kg),Middle(28.0 g/kg),and High(40.0 g/kg)dose in mice.At each time point 14 days,21 days,and 28 days,the body weight,liver coefficient,indexes of liver function alkaline phosphatase(ALP),alanine aminotransferase(ALT)and aspartate transaminase(AST),various biochemical biomarkers of liver tissue and mitochondria were detected and analyzed.Results:We found that W.E.R could decrease the body weight,increase the liver coefficient and the expression levels of indexes of liver function in mice.Next,we investigated that W.E.R could increase the expression levels of reactive oxygen species(ROS)and malondialdehyde(MDA),decrease the expression levels of adenosine triphosphate(ATP)and improve the enzyme activities of total superoxide dismutase(SOD).Third,we found that W.E.R could increase the enzyme activities of manganese-superoxide dismutase,decrease the enzyme activities of sodium-potassium adenosine triphosphatase(Na+-K+-ATPase)and calcium-magnesium adenosine triphosphatase(Ca 2+-Mg 2+-ATPase).Finally,we analyzed that there were significant negative correlations between body weight,expression level of ATP,activity of Na+-K+-ATPase,activity of Ca 2+-Mg 2+-ATPase and time,dose.There were significant positive correlations between liver coefficient,ALP,ALT,AST,expression levels of ROS,MDA and time,dose.Conclusion:All the results above indicated that W.E.R could cause the hepatotoxicity based on the oxidative damage in mice,which and mitochondria might be the main targets.展开更多
Objective: In this study, the hepatotoxic components of Polygoni multiflori Radix and Polygoni multiflori Radix Praeparata(known as Heshouwu [HSW] and Zhiheshouwu [ZHSW] in China, respectively) were screened, isolated...Objective: In this study, the hepatotoxic components of Polygoni multiflori Radix and Polygoni multiflori Radix Praeparata(known as Heshouwu [HSW] and Zhiheshouwu [ZHSW] in China, respectively) were screened, isolated, and identified. Materials and Methods: The ethanol extracts of HSW and ZHSW were separated into 80 fractions according to their polarity in the preparation liquid phase. Chang liver cell line was used to screen the toxic components of HSW and ZHSW in vitro. The obtained toxic mixture was further collected, isolated, and identified to confirm the hepatotoxic compounds of HSW and ZHSW. Results: The identifid hepatotoxic compounds include 2,3,5,4’-tetrah ydroxystilbene-2-O-β-D-glucoside, emodin, physcion-8-O-β-d-glucoside, physcion, and citreorosein, the fist two among them were the main components of HSW and ZHSW. After processing of HSW, the contents of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside and emodin in ZHSW were signifiantly decreased. Conclusions: The traditional processing with herb has signifiant effects on the components, especially the toxic components, in the extract of HSW and is an effective method to reduce its toxicity.展开更多
基金supported by The National Key Research and Development Plan 2019YFC1712403Major New Drug Discovery Science and Technology Major Projects 2017ZX09201002-006the Research Project of Capital Health and Development 2018-4-4231
文摘Background:Rhizoma Dioscoreae bulbiferae could cause liver damage,which limited its application in the clinic.Aims and Objectives:To explore the mechanism of hepatotoxicity of Rhizoma Dioscoreae bulbiferae in mice.Materials and Methods:In the present study,the water extraction of Rhizoma Dioscoreae bulbiferae(W.E.R)was administrated via intragastrical with Low(19.6 g/kg),Middle(28.0 g/kg),and High(40.0 g/kg)dose in mice.At each time point 14 days,21 days,and 28 days,the body weight,liver coefficient,indexes of liver function alkaline phosphatase(ALP),alanine aminotransferase(ALT)and aspartate transaminase(AST),various biochemical biomarkers of liver tissue and mitochondria were detected and analyzed.Results:We found that W.E.R could decrease the body weight,increase the liver coefficient and the expression levels of indexes of liver function in mice.Next,we investigated that W.E.R could increase the expression levels of reactive oxygen species(ROS)and malondialdehyde(MDA),decrease the expression levels of adenosine triphosphate(ATP)and improve the enzyme activities of total superoxide dismutase(SOD).Third,we found that W.E.R could increase the enzyme activities of manganese-superoxide dismutase,decrease the enzyme activities of sodium-potassium adenosine triphosphatase(Na+-K+-ATPase)and calcium-magnesium adenosine triphosphatase(Ca 2+-Mg 2+-ATPase).Finally,we analyzed that there were significant negative correlations between body weight,expression level of ATP,activity of Na+-K+-ATPase,activity of Ca 2+-Mg 2+-ATPase and time,dose.There were significant positive correlations between liver coefficient,ALP,ALT,AST,expression levels of ROS,MDA and time,dose.Conclusion:All the results above indicated that W.E.R could cause the hepatotoxicity based on the oxidative damage in mice,which and mitochondria might be the main targets.
文摘Objective: In this study, the hepatotoxic components of Polygoni multiflori Radix and Polygoni multiflori Radix Praeparata(known as Heshouwu [HSW] and Zhiheshouwu [ZHSW] in China, respectively) were screened, isolated, and identified. Materials and Methods: The ethanol extracts of HSW and ZHSW were separated into 80 fractions according to their polarity in the preparation liquid phase. Chang liver cell line was used to screen the toxic components of HSW and ZHSW in vitro. The obtained toxic mixture was further collected, isolated, and identified to confirm the hepatotoxic compounds of HSW and ZHSW. Results: The identifid hepatotoxic compounds include 2,3,5,4’-tetrah ydroxystilbene-2-O-β-D-glucoside, emodin, physcion-8-O-β-d-glucoside, physcion, and citreorosein, the fist two among them were the main components of HSW and ZHSW. After processing of HSW, the contents of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside and emodin in ZHSW were signifiantly decreased. Conclusions: The traditional processing with herb has signifiant effects on the components, especially the toxic components, in the extract of HSW and is an effective method to reduce its toxicity.
