Since it first appeared in 2022,the phenomenon referred to as Colony Collapse Disorder(CCD)has affected several regions of Morocco to varying degrees.In order to assess the possible impact of pesticides on the appeara...Since it first appeared in 2022,the phenomenon referred to as Colony Collapse Disorder(CCD)has affected several regions of Morocco to varying degrees.In order to assess the possible impact of pesticides on the appearance of this syndrome,we conducted a study aimed at evaluating the impact of pesticide use on the emergence of this syndrome through a year-long survey involving 160 beekeepers in the Beni Mellal–Khenifra Region(BKR)who also experienced an unprecedented desertion of hives during the same period.The majority of surveyed beekeepers practice mixed(45%)or migratory beekeeping(42%)and provide supplementary feeding(83.75%)to support their bees.Nearly 37.5%of the hives are located near crops treated with pesticides,exposing the bees to these chemicals.The results showed that the majority of beekeepers reported a cessation of queen laying(74.38%),high mortality rates among worker bees(81.25%),drones(65.63%),and queens(61.88%).Abnormal behaviors such as immobility with trembling(42.50%),reduced flights(47.50%),and disoriented navigation(28.75%)were also observed.Correlation analyses indicate that proximity to treated crops significantly increases the risk of queen laying cessation(Odds Ratio 6.0)and a reduction in waggle dances(Odds Ratio 2.41).Extended foraging flights show a borderline statistical significance(Odds Ratio 2.33),suggesting a disruption of natural food sources.These results highlight the potential impact of pesticides on colony health and bee behavior,pointing out the need to adapt beekeeping practices and implement protective measures against pesticides.展开更多
Aim： To evaluate for the first time the frequency of Y chromosome microdeletions and the occurrence of the partial deletions of AZFc region in Moroccan men, and to discuss the clinical significance of AZF deletions. ...Aim： To evaluate for the first time the frequency of Y chromosome microdeletions and the occurrence of the partial deletions of AZFc region in Moroccan men, and to discuss the clinical significance of AZF deletions. Methods： We screened Y chromosome microdeletions and partial deletions of the AZFc region of a consecutive group of infertile men （n = 149） and controls （100 fertile men, 76 normospermic men）. AZFa, AZFb, AZFc and partial deletions of the AZFc region were analyzed by polymerase chain reaction （PCR） according to established protocols. Results： Among the 127 infertile men screened for microdeletion, four subjects were found to have microdeletions： two AZFc deletions and two AZFb＋AZFc deletions. All the deletions were found only in azoospermic subjects （4/48, 8.33%）. The overall AZFc deletion frequency was low （4/127, 3.15%）. AZF microdeletions were not observed in either oligoasthenoteratozoospermia （OATS） or the control. Partial deletions of AZFc （gr/gr） were observed in a total of 7 of the 149 infertile men （4.70%） and 7 partial AZFc deletions （gr/gr） were found in the control group （7/176, 3.98%）. In addition, two b2/b3 deletions were identified in two azoospermic subjects （2/149, 1.34%） but not in the control group. Conclusion： Our results suggest that the frequency of Y chromosome AZF microdeletions is elevated in individuals with severe spermatogenic failure and that gr/gr deletions are not associated with spermatogenic failure.展开更多
This paper aims to assess soil fertility and risk contamination parameters in response to increased amendment rates of sewage sludge application,under semi-arid climatic conditions of Morocco,using two soil textures:s...This paper aims to assess soil fertility and risk contamination parameters in response to increased amendment rates of sewage sludge application,under semi-arid climatic conditions of Morocco,using two soil textures:soil A(clay-silty)and soil B(sandy-clay)over two years.To this end,sewage sludge and soils treatments physicochemical parameters,heavy metals content,degree of pollution and associated risks were evaluated and compared to controlled soils.The results showed that all studied parameters changed significantly(p<0.05)in a dose-dependent manner for both soils compared to the untreated control soils.Indeed,there were significant increase in organic matter(OM),total organic carbon(TOC)and nutrients content(N,P and K)of both sols as a response to increased sewage sludge amendment rates.Overall,sewage sludge application showed a positive agronomic potential for both soil and plant.However,there were concern about some heavy metals contamination risks,the results showed a gradual increase of heavy metals concentration in this order:Cu>Pb>Zn>Ni>Cd>Cr.Nevertheless,their concentrations were below the critical values established by the European Union for the agronomic use of soil.The obtained results of assessed parameters indicating SS degree of pollution and associated risks showed a low to a moderate contamination risks of heavy metals.Accordingly,environmental risks of sewage sludge application will be related to increase of heavy metals mainly in a long-term agricultural reuse.Thus,optimal management of sewage sludge application is recommended to ensure sustainability and benefits at the same time to master the contamination risks.展开更多
文摘Since it first appeared in 2022,the phenomenon referred to as Colony Collapse Disorder(CCD)has affected several regions of Morocco to varying degrees.In order to assess the possible impact of pesticides on the appearance of this syndrome,we conducted a study aimed at evaluating the impact of pesticide use on the emergence of this syndrome through a year-long survey involving 160 beekeepers in the Beni Mellal–Khenifra Region(BKR)who also experienced an unprecedented desertion of hives during the same period.The majority of surveyed beekeepers practice mixed(45%)or migratory beekeeping(42%)and provide supplementary feeding(83.75%)to support their bees.Nearly 37.5%of the hives are located near crops treated with pesticides,exposing the bees to these chemicals.The results showed that the majority of beekeepers reported a cessation of queen laying(74.38%),high mortality rates among worker bees(81.25%),drones(65.63%),and queens(61.88%).Abnormal behaviors such as immobility with trembling(42.50%),reduced flights(47.50%),and disoriented navigation(28.75%)were also observed.Correlation analyses indicate that proximity to treated crops significantly increases the risk of queen laying cessation(Odds Ratio 6.0)and a reduction in waggle dances(Odds Ratio 2.41).Extended foraging flights show a borderline statistical significance(Odds Ratio 2.33),suggesting a disruption of natural food sources.These results highlight the potential impact of pesticides on colony health and bee behavior,pointing out the need to adapt beekeeping practices and implement protective measures against pesticides.
文摘Aim： To evaluate for the first time the frequency of Y chromosome microdeletions and the occurrence of the partial deletions of AZFc region in Moroccan men, and to discuss the clinical significance of AZF deletions. Methods： We screened Y chromosome microdeletions and partial deletions of the AZFc region of a consecutive group of infertile men （n = 149） and controls （100 fertile men, 76 normospermic men）. AZFa, AZFb, AZFc and partial deletions of the AZFc region were analyzed by polymerase chain reaction （PCR） according to established protocols. Results： Among the 127 infertile men screened for microdeletion, four subjects were found to have microdeletions： two AZFc deletions and two AZFb＋AZFc deletions. All the deletions were found only in azoospermic subjects （4/48, 8.33%）. The overall AZFc deletion frequency was low （4/127, 3.15%）. AZF microdeletions were not observed in either oligoasthenoteratozoospermia （OATS） or the control. Partial deletions of AZFc （gr/gr） were observed in a total of 7 of the 149 infertile men （4.70%） and 7 partial AZFc deletions （gr/gr） were found in the control group （7/176, 3.98%）. In addition, two b2/b3 deletions were identified in two azoospermic subjects （2/149, 1.34%） but not in the control group. Conclusion： Our results suggest that the frequency of Y chromosome AZF microdeletions is elevated in individuals with severe spermatogenic failure and that gr/gr deletions are not associated with spermatogenic failure.
文摘This paper aims to assess soil fertility and risk contamination parameters in response to increased amendment rates of sewage sludge application,under semi-arid climatic conditions of Morocco,using two soil textures:soil A(clay-silty)and soil B(sandy-clay)over two years.To this end,sewage sludge and soils treatments physicochemical parameters,heavy metals content,degree of pollution and associated risks were evaluated and compared to controlled soils.The results showed that all studied parameters changed significantly(p<0.05)in a dose-dependent manner for both soils compared to the untreated control soils.Indeed,there were significant increase in organic matter(OM),total organic carbon(TOC)and nutrients content(N,P and K)of both sols as a response to increased sewage sludge amendment rates.Overall,sewage sludge application showed a positive agronomic potential for both soil and plant.However,there were concern about some heavy metals contamination risks,the results showed a gradual increase of heavy metals concentration in this order:Cu>Pb>Zn>Ni>Cd>Cr.Nevertheless,their concentrations were below the critical values established by the European Union for the agronomic use of soil.The obtained results of assessed parameters indicating SS degree of pollution and associated risks showed a low to a moderate contamination risks of heavy metals.Accordingly,environmental risks of sewage sludge application will be related to increase of heavy metals mainly in a long-term agricultural reuse.Thus,optimal management of sewage sludge application is recommended to ensure sustainability and benefits at the same time to master the contamination risks.
