This work is devoted to numerical analysis of thermo-hydromechanical problem and cracking process in saturated porous media in the context of deep geological disposal of radioactive waste.The fundamental background of...This work is devoted to numerical analysis of thermo-hydromechanical problem and cracking process in saturated porous media in the context of deep geological disposal of radioactive waste.The fundamental background of thermo-poro-elastoplasticity theory is first summarized.The emphasis is put on the effect of pore fluid pressure on plastic deformation.A micromechanics-based elastoplastic model is then presented for a class of clayey rocks considered as host rock.Based on linear and nonlinear homogenization techniques,the proposed model is able to systematically account for the influences of porosity and mineral composition on macroscopic elastic properties and plastic yield strength.The initial anisotropy and time-dependent deformation are also taken into account.The induced cracking process is described by using a non-local damage model.A specific hybrid formulation is proposed,able to conveniently capture tensile,shear and mixed cracks.In particular,the influences of pore pressure and confining stress on the shear cracking mechanism are taken into account.The proposed model is applied to investigating thermo-hydromechanical responses and induced damage evolution in laboratory tests at the sample scale.In the last part,an in situ heating experiment is analyzed by using the proposed model.Numerical results are compared with experimental data and field measurements in terms of temperature variation,pore fluid pressure change and induced damaged zone.展开更多
This paper addresses numerical analysis of thermo-hydromechanical processes in the context of deep geological disposal of radioactive waste.The emphasis is put on modeling of damaged zones induced by excavation,pore p...This paper addresses numerical analysis of thermo-hydromechanical processes in the context of deep geological disposal of radioactive waste.The emphasis is put on modeling of damaged zones induced by excavation,pore pressure and temperature changes.The theoretical background of thermo-poroelasticity for saturated porous media is first recalled.The framework for modeling initiation and evolution of induced cracks is then presented by using a variational approach of phase-field method.A specific model with two crack phase fields and considering thermo-hydromechanical interaction is proposed.A particular attention is paid on the description of shear cracks.The proposed model is implemented in the standard finite element method.An example of application is finally presented on the analysis of thermo-hydromechanical responses and cracked zones evolution around a typical disposal repository in the context of French concept for high level waste disposal.展开更多
In order to evaluate the performance of deep geological disposal of radioactive waste,an underground research laboratory(URL)was constructed by Andra in the Callovo-Oxfordian(COx)claystone formation at the Meuse/Haute...In order to evaluate the performance of deep geological disposal of radioactive waste,an underground research laboratory(URL)was constructed by Andra in the Callovo-Oxfordian(COx)claystone formation at the Meuse/Haute-Marne(MHM).The construction of URL induced the excavation damage of host formations,and the ventilation in the galleries desaturated the host formation close to the gallery wall.Moreover,it is expected that the mechanical behaviour of COx claystone is time-dependent.This study presents a constitutive model developed to describe the viscoplastic behaviour of unsaturated and damaged COx claystone.In this model,the unsaturation effect is considered by adopting the Bishop effective stress and the van Genuchten(VG)water retention model.In terms of the viscoplastic behaviour,the nonstationary flow surface(NSFS)theory for unsaturated soils is used with consideration of the coupled effects of strain rate and suction on the yield stress.A progressive hardening law is adopted.Meanwhile,a non-associated flow rule is used,which is similar to that in Barcelona basic model(BBM).In addition,to describe the damage effect induced by suction change and viscoplastic loading,a damage function is defined based on the crack volume proportion.This damage function contains two variables:unsaturated effective stress and viscoplastic volumetric strain,with the related parameters determined based on the mercury intrusion porosimetry(MIP)tests.For the model validation,different tests on COx claystone under different loading paths are simulated.Comparisons between experimental and simulated results indicated that the present model is able to well describe the viscoplastic behaviour of damaged COx claystone,including swelling/shrinkage,triaxial extension and compression,and triaxial creep.展开更多
On a global scale,lung cancer is responsible for around 27%of all cancer fatalities.Even though there have been great strides in diagnosis and therapy in recent years,the five-year cure rate is just 19%.Classification...On a global scale,lung cancer is responsible for around 27%of all cancer fatalities.Even though there have been great strides in diagnosis and therapy in recent years,the five-year cure rate is just 19%.Classification is crucial for diagnosing lung nodules.This is especially true today that automated categorization may provide a professional opinion that can be used by doctors.New computer vision and machine learning techniques have made possible accurate and quick categorization of CT images.This field of research has exploded in popularity in recent years because of its high efficiency and ability to decrease labour requirements.Here,they want to look carefully at the current state of automated categorization of lung nodules.Generalpurpose structures are briefly discussed,and typical algorithms are described.Our results show deep learning-based lung nodule categorization quickly becomes the industry standard.Therefore,it is critical to pay greater attention to the coherence of the data inside the study and the consistency of the research topic.Furthermore,there should be greater collaboration between designers,medical experts,and others in the field.展开更多
In the context of radioactive waste disposal,an underground research laboratory(URL)is a facility in which experiments are conducted to demonstrate the feasibility of constructing and operating a radioactive waste dis...In the context of radioactive waste disposal,an underground research laboratory(URL)is a facility in which experiments are conducted to demonstrate the feasibility of constructing and operating a radioactive waste disposal facility within a geological formation.The Meuse/Haute-Marne URL is a sitespecific facility planned to study the feasibility of a radioactive waste disposal in the Callovo-Oxfordian(COx)claystone.The thermo-hydro-mechanical(THM)behaviour of the host rock is significant for the design of the underground nuclear waste disposal facility and for its long-term safety.The French National Radioactive Waste Management Agency(Andra)has begun a research programme aiming to demonstrate the relevancy of the French high-level waste(HLW)concept.This paper presents the programme implemented from small-scale(small diameter)boreholes to full-scale demonstration experiments to study the THM effects of the thermal transient on the COx claystone and the strategy implemented in this new programme to demonstrate and optimise current disposal facility components for HLW.It shows that the French high-level waste concept is feasible and working in the COx claystone.It also exhibits that,as for other plastic clay or claystone,heating-induced pore pressure increases and that the THM behaviour is anisotropic.展开更多
Since 2000, the French National Radioactive Waste Management Agency （ANDRA） has been constructing an Underground Research Laboratory （URL） at Bure （east of the Paris Basin） to perform experiments in order to obt...Since 2000, the French National Radioactive Waste Management Agency （ANDRA） has been constructing an Underground Research Laboratory （URL） at Bure （east of the Paris Basin） to perform experiments in order to obtain in situ data necessary to demonstrate the feasibility of geological repository in the Callovo- Oxfordian claystone. An important experimental program is planned to characterize the response of the rock to different drift construction methods, Before 2008, at the main level of the laboratory, most of the drifts were excavated using pneumatic hammer and supported with rock bolts, sliding steel arches and fiber shotcrete. Other techniques, such as road header techniques, stiff and flexible supports, have also been used to characterize their impacts. The drift network is developed following the in situ major stresses. The parallel drifts are separated enough so as they can be considered independently when their hydromechanical （HM） behaviors are compared. Mine-by experiments have been performed to measure the HM response of the rock and the mechanical loading applied to the support system due to the digging and after excavation. Drifts exhibit extensional （mode I） and shear fractures （modes II and III） induced by excavation works. The extent of the induced fracture networks depends on the drift orientation versus the in situ stress field. This paper describes the drift convergence and deformation in the surrounding rock walls as function of time and the impact of different support methods on the rock mass behavior. An observation based method is finally applied to distinguish the instantaneous and time-dependent parts of the rock mass deformation around the drifts.展开更多
Self-sealing of fractures in the indurated Callovo-Oxfordian(COX)and Opalinus(OPA)claystones,which are considered as host rocks for disposal of radioactive waste,was investigated on artificially fractured samples.The ...Self-sealing of fractures in the indurated Callovo-Oxfordian(COX)and Opalinus(OPA)claystones,which are considered as host rocks for disposal of radioactive waste,was investigated on artificially fractured samples.The samples were extracted from four lithological facies relatively rich in clay mineral,carbonate and quartz,respectively.The self-sealing of fractures was measured by fracture closure,water permeability variation,gas penetration,and recovery of gas-induced pathways.Most of the fractured samples exhibited a dramatic reduction inwater permeability to low levels that is close to that of intact rock,depending on their mineralogical composition,fracture intensity,confining stress,and load duration.The self-sealing capacity of the clay-rich samples is higher than that of the carbonate-rich and sandy ones.Significant effects of sample size and fracture intensity were identified.The sealed fractures become gas-tight for certain in-jection pressures.However,the measured gas breakthrough pressures are still lower than the confining stresses.The gas-induced pathways can recover when contacting water.These important findings imply that fractures in such indurated claystones can effectively recover to hinder water transport but allow gas release under relatively low pressures without compromising the rock integrity.展开更多
Callovo-Oxfordian(COx)claystone has been considered as a potential host rock for geological radioactive waste disposal in France(Cigéo project).During the exploitation phase(100 years),the stability of drifts(e.g...Callovo-Oxfordian(COx)claystone has been considered as a potential host rock for geological radioactive waste disposal in France(Cigéo project).During the exploitation phase(100 years),the stability of drifts(e.g.galleries/alveoli)within the disposal is assured by the liner,which includes two layers:concrete arch segment and compressible material.The latter exhibits a significant deformation capacity(about 50%)under low stress(<3 MPa).Although the response of these underground structures can be governed by complex thermo-hydro-mechanical coupling,the creep behavior of COx claystone has been considered as the main factor controlling the increase of stress state in the concrete liner and hence the long-term stability of drifts.Therefore,by focusing only on the purely mechanical behavior,this study aims at investigating the uncertainty effect of the COx claystone time-dependent properties on the stability of an alveolus of Cigéo during the exploitation period.To describe the creep behavior of COx claystone,we use Lemaitre’s viscoplastic model with three parameters whose uncertainties are identified from laboratory creep tests.For the reliability analysis,an extension of a well-known Kriging metamodeling technique is proposed to assess the exceedance probability of acceptable stress in the concrete liner of the alveolus.The open-source code Code_Aster is chosen for the direct numerical evaluations of the performance function.The Kriging-based reliability analysis elucidates the effect of the uncertainty of COx claystone on the long-term stability of the concrete liner.Moreover,the role of the compressible material layer between the concrete liner and the host rock is also highlighted.展开更多
Thermal effects on the Callovo-Oxfordian and Opalinus clay rocks for hosting high-level radioactive waste were comprehensively investigated with laboratory and in situ experiments under repository relevant conditions:...Thermal effects on the Callovo-Oxfordian and Opalinus clay rocks for hosting high-level radioactive waste were comprehensively investigated with laboratory and in situ experiments under repository relevant conditions:(1) stresses covering the range from the initial lithostatic state to redistributed levels after excavation,(2) hydraulic drained and undrained boundaries, and(3) heating from ambient temperature up to 90℃-120℃ and a subsequent cooling phase. The laboratory experiments were performed on normal-sized and large hollow cylindrical samples in various respects of thermal expansion and contraction, thermally-induced pore water pressure, temperature influences on deformation and strength, thermal impacts on swelling, fracture sealing and permeability. The laboratory results obtained from the samples are consistent with the in situ observations during heating experiments in the underground research laboratories at Bure and Mont-Terri. Even though the claystones showed significant responses to thermal loading, no negative effects on their favorable barrier properties were observed.展开更多
In the French deep geological disposal for radioactive wastes,compacted bentonite/claystone mixtures have been considered as possible sealing materials.After emplacement in place,such mixtures are hydrated by the site...In the French deep geological disposal for radioactive wastes,compacted bentonite/claystone mixtures have been considered as possible sealing materials.After emplacement in place,such mixtures are hydrated by the site solution as well as the cement solution produced by the degradation of concrete.In this study,the effects of synthetic site solution and cement solution on the hydro-mechanical behaviour of compacted mixtures of claystone and two types of bentonites(MX80 Na-bentonite and Sardinia Cabentonite)were investigated by carrying out a series of swelling pressure,hydraulic conductivity and mercury intrusion porosimetry(MIP)tests.It was found that for the MX80 bentonite/claystone mixture hydrated with synthetic site solution,the swelling capacity was reduced compared to the case with deionised water owing to the transformation of Na-montmorillonite to multi-cation dominant montmorillonite by cation exchanges.For the Sardinia bentonite/claystone mixture,the similar increasing rate of swelling pressure was observed during the crystalline swelling process for different solutions,suggesting insignificant cation exchanges.Additionally,the cations in the synthetic site solution could reduce the thickness of diffuse double layer and the osmotic swelling for both MX80 bentonite/claystone and Sardinia bentonite/claystone mixtures.The large-pore volume increased consequently and enhanced water flow.In the cement solution,the hydroxide could also dissolve the montmorillonite,reducing the swelling pressure,and increase the large-pore volume,facilitating the water flow.Furthermore,the decrease of swelling pressure and the increase of hydraulic conductivity were more significant in the case of low dry density because of more intensive interaction between montmorillonite and hydroxide due to the high permeability.展开更多
In the context of the Cigeo project,the French National Radioactive Waste Management Agency(Andra)is studying the behaviour of a deep geological facility for radioactive waste deposit in the CallovoOxfordian(COx)clays...In the context of the Cigeo project,the French National Radioactive Waste Management Agency(Andra)is studying the behaviour of a deep geological facility for radioactive waste deposit in the CallovoOxfordian(COx)claystone.The assessment of durability of this project requires the prediction of irreversible strain over a large time scale.The mechanical interaction of the host rock and the concrete support of tunnels must be investigated to ensure the long-term sustainability of the structure.The instantaneous and time-dependent behaviour of the claystone-concrete interface is experimentally investigated with direct shear tests and long-duration shear tests of a few months.The mechanical and structural state of the claystone which is affected after interaction with concrete reflects to the response of the claystone-concrete interface,and thus different types of COx claystone-concrete interfaces are tested.The delayed deformation of the interface is found to be linked to the level of the normal loading and the loading history while a different response of the interface was observed from the short-and long-duration tests,indicating a possible progressive modification of interface under long-duration loadings.展开更多
This paper introduced a novel microstructure-based constitutive model designed to comprehensively characterize the intricate mechanical behavior of anisotropic clay rocks under the influence of water saturation.The pr...This paper introduced a novel microstructure-based constitutive model designed to comprehensively characterize the intricate mechanical behavior of anisotropic clay rocks under the influence of water saturation.The proposed model encompasses elastoplastic deformation,time-dependent behavior,and induced damage.A two-step homogenization process incorporates mineral compositions and porosity to determine the macroscopic elastic tensor and plastic yield criterion.The model also considers interfacial debonding between the matrix and inclusions to capture rock damage.The application of the proposed model is demonstrated through an analysis of Callovo-Oxfordian clayey rocks,specifically in the context of radioactive waste disposal in France.Model parameters are determined,followed by numerical simulations of various laboratory tests including lateral decompression tests with constant mean stress,triaxial compression tests under different water saturation conditions,and creep tests.The numerical results are compared with corresponding experimental data to assess the efficacy of the proposed model.展开更多
Embryonic stem(ES)cells have the ability to differ-entiate into all germ layers,holding great promise not only for a model of early embryonic development but also for a robust cell source for cell-replacement therapie...Embryonic stem(ES)cells have the ability to differ-entiate into all germ layers,holding great promise not only for a model of early embryonic development but also for a robust cell source for cell-replacement therapies and for drug screening.Embryoid body (EB)formation from ES cells is a common method for producing different cell lineages for further applications. However,conventional techniques such as hanging drop or static suspension culture are either inherently incapable of large scale production or exhibit limited control over cell aggregation during EB formation and subsequent EB aggregation.For standardized mass EB production,a well defined scale-up platform is necessary.Recently,novel scenario methods of EB formation in hydrodynamic conditions created by bioreactor culture systems using stirred suspension systems(spinner flasks),rotating cell culture system and rotary orbital culture have allowed large-scale EB formation.Their use allows for continuous monitoring and control of the physical and chemical environment which is difficult to achieve by traditional methods.This review summarizes the current state of production of EBs derived from pluripotent cells in various culture systems.Furthermore,an overview of high quality EB formation strategies coupled with systems for in vitro differentiation into various cell types to be applied in cell replacement therapy is provided in this review. Recently,new insights in induced pluripotent stem(iPS) cell technology showed that differentiation and lineage commitment are not irreversible processes and this has opened new avenues in stem cell research.These cells are equivalent to ES cells in terms of both self-renewal and differentiation capacity.Hence,culture systems for expansion and differentiation of iPS cells can also apply methodologies developed with ES cells,although direct evidence of their use for iPS cells is still limited.展开更多
AIM To investigate the impact of medication beliefs,illness perceptions and quality of life on medication adherence in people with decompensated cirrhosis.METHODS One hundred adults with decompensated cirrhosis comple...AIM To investigate the impact of medication beliefs,illness perceptions and quality of life on medication adherence in people with decompensated cirrhosis.METHODS One hundred adults with decompensated cirrhosis completed a structured questionnaire when they attended for routine outpatient hepatology review.Measures of self-reported medication adherence(Morisky Medication Adherence Scale),beliefs surrounding medications(Beliefs about Medicines Questionnaire),perceptions of illness and medicines(Brief Illness Perception Questionnaire),and quality of life(Chronic Liver Disease Questionnaire)were examined.Clinical data were obtained via patient history and review of medical records.Least absolute shrinkage and selection operator and stepwise backwards regression techniques were used to construct the multivariable logistic regression model.Statistical significance was set at alpha=0.05.RESULTS Medication adherence was"High"in 42%o f participants,"Medium"in 37%,and"Low"in 21%.Compared to patients with"High"adherence,those with"Medium"or"Low"adherence were more likely to report difficulty affording their medications(P<0.001),lower perception of treatment helpfulness(P=0.003)and stronger medication concerns relative to medication necessity beliefs(P=0.003).People with"Low"adherence also experienced greater symptom burden and poorer quality of life,including more frequent abdominal pain(P=0.023),shortness of breath(P=0.030),and emotional disturbances(P=0.050).Multivariable analysis identified having stronger medication concerns relative to necessity beliefs(Necessity-Concerns Differential≤5,OR=3.66,95%CI:1.18-11.40)and more frequent shortness of breath(shortness of breath score≤3,OR=3.87,95%CI:1.22-12.25)as independent predictors of"Low"adherence.CONCLUSION The association between"Low"adherence and patients having strong concerns or doubting the necessity or helpfulness of their medications should be explored further given the clinical relevance.展开更多
This paper presents the main results obtained during a decade of scientific activities in the Meuse/Haute-Marne Underground Research Laboratory （URL） located on the eastern boundary of the Paris Basin, in the Callov...This paper presents the main results obtained during a decade of scientific activities in the Meuse/Haute-Marne Underground Research Laboratory （URL） located on the eastern boundary of the Paris Basin, in the Callovo-Oxfordian clay rock formation. The URL was built in the framework of ANDRA＇s research program into the feasibility of a reversible deep geological disposal of high-level and intermediate-level long-lived radioactive （HL, ILLL） waste. Its underground drifts have been used to study a 160-million-year old clay layer. The 2006 Planning Act adopted this disposal concept as the reference solution for the long-term management of HL and ILLL radioactive waste. Today, research is continuing into the design and sitting of the disposal facility which could be commissioned by 2025 if its license is granted in 2016. Through these programs, the laboratory will help ANDRA develop a concrete approach with a view to proposing suitable architectures and management methods for a deep disposal facility, to allow by 2016 the decision for the start of the construction of the shafts and drifts of the new disposal facility.展开更多
Within the framework of feasibility studies for a reversible, deep geological repository of high-and intermediate-level long-lived radioactive waste(HLW, IL-LLW), the French National Radioactive Waste Management Agenc...Within the framework of feasibility studies for a reversible, deep geological repository of high-and intermediate-level long-lived radioactive waste(HLW, IL-LLW), the French National Radioactive Waste Management Agency(Andra) is investigating the Callovo-Oxfordian(COx) formation near Bure(northeast part of France) as a potential host rock for the repository. The hydro-mechanical(HM) behaviour is an important issue to design and optimise different components of the disposal such as shaft, ramp, drift,and waste package disposal facilities. Over the past 20 years, a large number of laboratory experiments have been carried out to characterise and understand the HM behaviours of COx claystones. At the beginning, samples came from deep boreholes drilled at the ground surface with oil base mud. From2000 onwards, with the launch of the construction of the Meuse/Haute-Marne Underground Research Laboratory(MHM URL), most samples have been extracted from a large number of air drilled boreholes in the URL. In parallel, various constitutive models have been developed for modelling. The thermohydro-mechanical(THM) behaviours of the COx claystones were investigated under different repository conditions. Core samples are subjected to a complex HM loading path before testing, due to drilling, conditioning and preparation. Various kinds of effects on the characteristics of the claystones are highlighted and discussed, and the procedures for core extraction and packaging as well as a systematic sample preparation protocol are proposed in order to minimise the uncertainties on test results. The representativeness of the test results is also addressed with regard to the in situ rock mass.展开更多
Brown planthopper （Nilaparvata lugens Stal） is one of the most damaging pests causing hopper burn in rice, and thereby reducing the productivity and also the quality of the product. The effective management strategy...Brown planthopper （Nilaparvata lugens Stal） is one of the most damaging pests causing hopper burn in rice, and thereby reducing the productivity and also the quality of the product. The effective management strategy to control this pest is the identification and transfer of desirable genes to local rice cultivars. The most important approach for developing resistant cultivars is the identification of markers, which can help in marker-assisted selection of more durable resistant genotype. The susceptible parent IR50 and the resistant parent Ptb33, and their F2 populations were used in bulked segregant analysis for identification of resistant genes with random amplified polymorphic DNA marker （RAPD） primers. The primers OPC7 and OPAG14 showed both dominant and susceptible specific banding pattern so called co-dominant markers. Moreover, OPC7697 and OPAG14680 showed resistant specific bands and thus being in coupling phase, whereas OPC7846 and OPAG14650 showed susceptible specific genotypic bands in bulked segregant analysis. Therefore, the coupling phase markers, OPC7697 and OPAG14680, are considered to be more useful in marker-assisted selection of rice genotypes in crop improvement.展开更多
The French National Agency for Nuclear Waste Management (Andra) conducted a site investiga- tions program within the project of a deep geological disposal of radioactive waste in the Meuse/ Haute-Marne region. The con...The French National Agency for Nuclear Waste Management (Andra) conducted a site investiga- tions program within the project of a deep geological disposal of radioactive waste in the Meuse/ Haute-Marne region. The construction of the tunnel of 5 Km length and the shafts of about 500 m depth to access the repository located in the clay host formation of Callovo-Oxfordian age, will lead to the perturbations of the groundwater flow fields. The prediction of the behaviour of these perturbations is needed to support: 1) the engineering and monitoring operations, and 2) the assessment of the consequences on groundwater resources. A variably-saturated flow model of a local multi-layered aquifer system is developed. It integrates the Oxfordian aquifer (limestone), the Kimmeridgianaquitard (marl) and the Barrois limestone aquifer including the karst conduits network. The variably-saturated flow Richard’s equation is solved with a finite element simulator. Prior to the simulation of the predictive repository impacts, a transient flow model is calibrated with respect to Underground Research Laboratory (URL) construction data. The results are analysed and evaluated by the use of performance measures.展开更多
AIM:To compare gene targeting efficiencies,expression profiles,and Ca2+ handling potentials in two widely used mouse embryonic stem cell lines.METHODS:The two widely used mouse embryonic stem cell lines,R1 and HM-1,we...AIM:To compare gene targeting efficiencies,expression profiles,and Ca2+ handling potentials in two widely used mouse embryonic stem cell lines.METHODS:The two widely used mouse embryonic stem cell lines,R1 and HM-1,were cultured and maintained on Mitomycin C treated mouse embryonic fibroblast feeder cell layers,following standard culture procedures.Cells were incubated with primary and secondary antibodies before fluorescence activated cell sorting analysis to compare known pluripotency markers.Moreover,cells were harvested by trypsinization and transfected with a kinase-inactive murine Tyk2 targeting construct,following the BioRad and Amaxa transfection procedures.Subsequently,the cells were cultured and neomycin-resistant cells were picked after 13 d of selection.Surviving clones were screened twice by polymerase chain reaction(PCR) and finally confirmed by Southern blot analysis before comparison.Global gene expression profiles of more than 20 400 probes were also compared and significantly regulated genes were conf irmed by real time PCR analysis.Calcium handling potentials of these cell lines were also compared using various agonists.RESULTS:We found signif icant differences in transfection eff iciencies of the two cell lines(91% ± 6.1% vs 75% ± 4.2%,P = 0.01).Differences in the targeting efficiencies were also significant whether the Amaxa or BioRad platforms were used for comparison.We did not observe signif icant differences in the levels of many known pluripotency markers.However,our genomewide expression analysis using more than 20 400 spotted cDNA arrays identified 55 differentially regulated transcripts(P < 0.05) implicated in various important biological processes,including binding molecular functions(particularly Ca2+ binding roles).Subsequently,we measured Ca2+ signals in these cell lines in response to various calcium agonists,both in high and low Ca2+ solutions,and found signif icant differences(P < 0.05) in the regulation of Ca2+ homeostasis between the investigated cell lines.Then we further compared the detection and expression of various membrane and in-tracellular Ca2+ receptors and similarly found signif icant(P < 0.05) variations in a number of calcium receptors between these cell lines.CONCLUSION:Results of this study emphasize the importance of considering intrinsic cellular variations,during selection of cell lines for experiments and interpretations of experimental results.展开更多
Spondylolisthesis is a chronic disease,and a timely diagnosis of it may help in avoiding surgery.Disease identification in x-ray radiographs is very challenging.Strengthening the feature extraction tool in VGG16 has i...Spondylolisthesis is a chronic disease,and a timely diagnosis of it may help in avoiding surgery.Disease identification in x-ray radiographs is very challenging.Strengthening the feature extraction tool in VGG16 has improved the classification rate.But the fully connected layers of VGG16 are not efficient at capturing the positional structure of an object in images.Capsule network(CapsNet)works with capsules(neuron clusters)rather than a single neuron to grasp the properties of the provided image to match the pattern.In this study,an integrated model that is a combination of VGG16 and CapsNet(S-VCNet)is proposed.In the model,VGG16 is used as a feature extractor.After feature extraction,the output is fed to CapsNet for disease identification.A private dataset is used that contains 466 X-ray radiographs,including 186 images displaying a spine with spondylolisthesis and 280 images depicting a normal spine.The suggested model is the first step towards developing a web-based radiological diagnosis tool that can be utilized in outpatient clinics where there are not enough qualified medical professionals.Experimental results demonstrate that the developed model outperformed the other models that are used for lumbar spondylolisthesis diagnosis with 98%accuracy.After the performance check,the model has been successfully deployed on the Gradio web app platform to produce the outcome in less than 20 s.展开更多
基金supported by the French National Agency for radioactive waste management(ANDRA).
文摘This work is devoted to numerical analysis of thermo-hydromechanical problem and cracking process in saturated porous media in the context of deep geological disposal of radioactive waste.The fundamental background of thermo-poro-elastoplasticity theory is first summarized.The emphasis is put on the effect of pore fluid pressure on plastic deformation.A micromechanics-based elastoplastic model is then presented for a class of clayey rocks considered as host rock.Based on linear and nonlinear homogenization techniques,the proposed model is able to systematically account for the influences of porosity and mineral composition on macroscopic elastic properties and plastic yield strength.The initial anisotropy and time-dependent deformation are also taken into account.The induced cracking process is described by using a non-local damage model.A specific hybrid formulation is proposed,able to conveniently capture tensile,shear and mixed cracks.In particular,the influences of pore pressure and confining stress on the shear cracking mechanism are taken into account.The proposed model is applied to investigating thermo-hydromechanical responses and induced damage evolution in laboratory tests at the sample scale.In the last part,an in situ heating experiment is analyzed by using the proposed model.Numerical results are compared with experimental data and field measurements in terms of temperature variation,pore fluid pressure change and induced damaged zone.
基金supported by the French National Agency for radioactive waste management(ANDRA)and the National Natural Science Foundation of China(No.12202099).
文摘This paper addresses numerical analysis of thermo-hydromechanical processes in the context of deep geological disposal of radioactive waste.The emphasis is put on modeling of damaged zones induced by excavation,pore pressure and temperature changes.The theoretical background of thermo-poroelasticity for saturated porous media is first recalled.The framework for modeling initiation and evolution of induced cracks is then presented by using a variational approach of phase-field method.A specific model with two crack phase fields and considering thermo-hydromechanical interaction is proposed.A particular attention is paid on the description of shear cracks.The proposed model is implemented in the standard finite element method.An example of application is finally presented on the analysis of thermo-hydromechanical responses and cracked zones evolution around a typical disposal repository in the context of French concept for high level waste disposal.
文摘In order to evaluate the performance of deep geological disposal of radioactive waste,an underground research laboratory(URL)was constructed by Andra in the Callovo-Oxfordian(COx)claystone formation at the Meuse/Haute-Marne(MHM).The construction of URL induced the excavation damage of host formations,and the ventilation in the galleries desaturated the host formation close to the gallery wall.Moreover,it is expected that the mechanical behaviour of COx claystone is time-dependent.This study presents a constitutive model developed to describe the viscoplastic behaviour of unsaturated and damaged COx claystone.In this model,the unsaturation effect is considered by adopting the Bishop effective stress and the van Genuchten(VG)water retention model.In terms of the viscoplastic behaviour,the nonstationary flow surface(NSFS)theory for unsaturated soils is used with consideration of the coupled effects of strain rate and suction on the yield stress.A progressive hardening law is adopted.Meanwhile,a non-associated flow rule is used,which is similar to that in Barcelona basic model(BBM).In addition,to describe the damage effect induced by suction change and viscoplastic loading,a damage function is defined based on the crack volume proportion.This damage function contains two variables:unsaturated effective stress and viscoplastic volumetric strain,with the related parameters determined based on the mercury intrusion porosimetry(MIP)tests.For the model validation,different tests on COx claystone under different loading paths are simulated.Comparisons between experimental and simulated results indicated that the present model is able to well describe the viscoplastic behaviour of damaged COx claystone,including swelling/shrinkage,triaxial extension and compression,and triaxial creep.
文摘On a global scale,lung cancer is responsible for around 27%of all cancer fatalities.Even though there have been great strides in diagnosis and therapy in recent years,the five-year cure rate is just 19%.Classification is crucial for diagnosing lung nodules.This is especially true today that automated categorization may provide a professional opinion that can be used by doctors.New computer vision and machine learning techniques have made possible accurate and quick categorization of CT images.This field of research has exploded in popularity in recent years because of its high efficiency and ability to decrease labour requirements.Here,they want to look carefully at the current state of automated categorization of lung nodules.Generalpurpose structures are briefly discussed,and typical algorithms are described.Our results show deep learning-based lung nodule categorization quickly becomes the industry standard.Therefore,it is critical to pay greater attention to the coherence of the data inside the study and the consistency of the research topic.Furthermore,there should be greater collaboration between designers,medical experts,and others in the field.
文摘In the context of radioactive waste disposal,an underground research laboratory(URL)is a facility in which experiments are conducted to demonstrate the feasibility of constructing and operating a radioactive waste disposal facility within a geological formation.The Meuse/Haute-Marne URL is a sitespecific facility planned to study the feasibility of a radioactive waste disposal in the Callovo-Oxfordian(COx)claystone.The thermo-hydro-mechanical(THM)behaviour of the host rock is significant for the design of the underground nuclear waste disposal facility and for its long-term safety.The French National Radioactive Waste Management Agency(Andra)has begun a research programme aiming to demonstrate the relevancy of the French high-level waste(HLW)concept.This paper presents the programme implemented from small-scale(small diameter)boreholes to full-scale demonstration experiments to study the THM effects of the thermal transient on the COx claystone and the strategy implemented in this new programme to demonstrate and optimise current disposal facility components for HLW.It shows that the French high-level waste concept is feasible and working in the COx claystone.It also exhibits that,as for other plastic clay or claystone,heating-induced pore pressure increases and that the THM behaviour is anisotropic.
文摘Since 2000, the French National Radioactive Waste Management Agency （ANDRA） has been constructing an Underground Research Laboratory （URL） at Bure （east of the Paris Basin） to perform experiments in order to obtain in situ data necessary to demonstrate the feasibility of geological repository in the Callovo- Oxfordian claystone. An important experimental program is planned to characterize the response of the rock to different drift construction methods, Before 2008, at the main level of the laboratory, most of the drifts were excavated using pneumatic hammer and supported with rock bolts, sliding steel arches and fiber shotcrete. Other techniques, such as road header techniques, stiff and flexible supports, have also been used to characterize their impacts. The drift network is developed following the in situ major stresses. The parallel drifts are separated enough so as they can be considered independently when their hydromechanical （HM） behaviors are compared. Mine-by experiments have been performed to measure the HM response of the rock and the mechanical loading applied to the support system due to the digging and after excavation. Drifts exhibit extensional （mode I） and shear fractures （modes II and III） induced by excavation works. The extent of the induced fracture networks depends on the drift orientation versus the in situ stress field. This paper describes the drift convergence and deformation in the surrounding rock walls as function of time and the impact of different support methods on the rock mass behavior. An observation based method is finally applied to distinguish the instantaneous and time-dependent parts of the rock mass deformation around the drifts.
基金This work was co-funded by the German Federal Ministry for Economic Affairs and Energy(BMWi)under contract number 02E11627by the European Commission(EC)from the Euro-pean Union’s Horizon 2020 research and innovationprogram under Grant No.847593.
文摘Self-sealing of fractures in the indurated Callovo-Oxfordian(COX)and Opalinus(OPA)claystones,which are considered as host rocks for disposal of radioactive waste,was investigated on artificially fractured samples.The samples were extracted from four lithological facies relatively rich in clay mineral,carbonate and quartz,respectively.The self-sealing of fractures was measured by fracture closure,water permeability variation,gas penetration,and recovery of gas-induced pathways.Most of the fractured samples exhibited a dramatic reduction inwater permeability to low levels that is close to that of intact rock,depending on their mineralogical composition,fracture intensity,confining stress,and load duration.The self-sealing capacity of the clay-rich samples is higher than that of the carbonate-rich and sandy ones.Significant effects of sample size and fracture intensity were identified.The sealed fractures become gas-tight for certain in-jection pressures.However,the measured gas breakthrough pressures are still lower than the confining stresses.The gas-induced pathways can recover when contacting water.These important findings imply that fractures in such indurated claystones can effectively recover to hinder water transport but allow gas release under relatively low pressures without compromising the rock integrity.
文摘Callovo-Oxfordian(COx)claystone has been considered as a potential host rock for geological radioactive waste disposal in France(Cigéo project).During the exploitation phase(100 years),the stability of drifts(e.g.galleries/alveoli)within the disposal is assured by the liner,which includes two layers:concrete arch segment and compressible material.The latter exhibits a significant deformation capacity(about 50%)under low stress(<3 MPa).Although the response of these underground structures can be governed by complex thermo-hydro-mechanical coupling,the creep behavior of COx claystone has been considered as the main factor controlling the increase of stress state in the concrete liner and hence the long-term stability of drifts.Therefore,by focusing only on the purely mechanical behavior,this study aims at investigating the uncertainty effect of the COx claystone time-dependent properties on the stability of an alveolus of Cigéo during the exploitation period.To describe the creep behavior of COx claystone,we use Lemaitre’s viscoplastic model with three parameters whose uncertainties are identified from laboratory creep tests.For the reliability analysis,an extension of a well-known Kriging metamodeling technique is proposed to assess the exceedance probability of acceptable stress in the concrete liner of the alveolus.The open-source code Code_Aster is chosen for the direct numerical evaluations of the performance function.The Kriging-based reliability analysis elucidates the effect of the uncertainty of COx claystone on the long-term stability of the concrete liner.Moreover,the role of the compressible material layer between the concrete liner and the host rock is also highlighted.
基金funding by the German Federal Ministry of Economics and Technology (BMWi) under contract No.02E10377the French National Radioactive Waste Management Agency (Andra)
文摘Thermal effects on the Callovo-Oxfordian and Opalinus clay rocks for hosting high-level radioactive waste were comprehensively investigated with laboratory and in situ experiments under repository relevant conditions:(1) stresses covering the range from the initial lithostatic state to redistributed levels after excavation,(2) hydraulic drained and undrained boundaries, and(3) heating from ambient temperature up to 90℃-120℃ and a subsequent cooling phase. The laboratory experiments were performed on normal-sized and large hollow cylindrical samples in various respects of thermal expansion and contraction, thermally-induced pore water pressure, temperature influences on deformation and strength, thermal impacts on swelling, fracture sealing and permeability. The laboratory results obtained from the samples are consistent with the in situ observations during heating experiments in the underground research laboratories at Bure and Mont-Terri. Even though the claystones showed significant responses to thermal loading, no negative effects on their favorable barrier properties were observed.
基金supported by Ecole des Ponts Paris Tech and the French National Radioactive Waste Management Agency(Andra)China Scholarship Council for the grant scholarship。
文摘In the French deep geological disposal for radioactive wastes,compacted bentonite/claystone mixtures have been considered as possible sealing materials.After emplacement in place,such mixtures are hydrated by the site solution as well as the cement solution produced by the degradation of concrete.In this study,the effects of synthetic site solution and cement solution on the hydro-mechanical behaviour of compacted mixtures of claystone and two types of bentonites(MX80 Na-bentonite and Sardinia Cabentonite)were investigated by carrying out a series of swelling pressure,hydraulic conductivity and mercury intrusion porosimetry(MIP)tests.It was found that for the MX80 bentonite/claystone mixture hydrated with synthetic site solution,the swelling capacity was reduced compared to the case with deionised water owing to the transformation of Na-montmorillonite to multi-cation dominant montmorillonite by cation exchanges.For the Sardinia bentonite/claystone mixture,the similar increasing rate of swelling pressure was observed during the crystalline swelling process for different solutions,suggesting insignificant cation exchanges.Additionally,the cations in the synthetic site solution could reduce the thickness of diffuse double layer and the osmotic swelling for both MX80 bentonite/claystone and Sardinia bentonite/claystone mixtures.The large-pore volume increased consequently and enhanced water flow.In the cement solution,the hydroxide could also dissolve the montmorillonite,reducing the swelling pressure,and increase the large-pore volume,facilitating the water flow.Furthermore,the decrease of swelling pressure and the increase of hydraulic conductivity were more significant in the case of low dry density because of more intensive interaction between montmorillonite and hydroxide due to the high permeability.
文摘In the context of the Cigeo project,the French National Radioactive Waste Management Agency(Andra)is studying the behaviour of a deep geological facility for radioactive waste deposit in the CallovoOxfordian(COx)claystone.The assessment of durability of this project requires the prediction of irreversible strain over a large time scale.The mechanical interaction of the host rock and the concrete support of tunnels must be investigated to ensure the long-term sustainability of the structure.The instantaneous and time-dependent behaviour of the claystone-concrete interface is experimentally investigated with direct shear tests and long-duration shear tests of a few months.The mechanical and structural state of the claystone which is affected after interaction with concrete reflects to the response of the claystone-concrete interface,and thus different types of COx claystone-concrete interfaces are tested.The delayed deformation of the interface is found to be linked to the level of the normal loading and the loading history while a different response of the interface was observed from the short-and long-duration tests,indicating a possible progressive modification of interface under long-duration loadings.
文摘This paper introduced a novel microstructure-based constitutive model designed to comprehensively characterize the intricate mechanical behavior of anisotropic clay rocks under the influence of water saturation.The proposed model encompasses elastoplastic deformation,time-dependent behavior,and induced damage.A two-step homogenization process incorporates mineral compositions and porosity to determine the macroscopic elastic tensor and plastic yield criterion.The model also considers interfacial debonding between the matrix and inclusions to capture rock damage.The application of the proposed model is demonstrated through an analysis of Callovo-Oxfordian clayey rocks,specifically in the context of radioactive waste disposal in France.Model parameters are determined,followed by numerical simulations of various laboratory tests including lateral decompression tests with constant mean stress,triaxial compression tests under different water saturation conditions,and creep tests.The numerical results are compared with corresponding experimental data to assess the efficacy of the proposed model.
基金Supported by Grants from EU FP6("MEDRAT"-LSHG-CT-2005-518240"CLONET",MRTN-CT-2006-035468),EU FP7("Partn ErS",PIAP-GA-2008-218205+6 种基金"InduHeart",EU FP7-PEOPLE-IRG-2008-234390"InduStem",PIAP-GA-2008-230675"Plurisys",HEALTH-F4-2009-223485)NKFP_07_1-ES2HEART-HU,No.OM-00202-2007 CHE-TRF senior scholarship,No.RTA 5080010supported by grant under the program Strategic Scholarships for Frontier Research Network for the Joint Ph.D.Program Thai Doctoral degree from the Office of the Higher Education Commission,Thailand,No.CHE-PhD-SW-2005-100
文摘Embryonic stem(ES)cells have the ability to differ-entiate into all germ layers,holding great promise not only for a model of early embryonic development but also for a robust cell source for cell-replacement therapies and for drug screening.Embryoid body (EB)formation from ES cells is a common method for producing different cell lineages for further applications. However,conventional techniques such as hanging drop or static suspension culture are either inherently incapable of large scale production or exhibit limited control over cell aggregation during EB formation and subsequent EB aggregation.For standardized mass EB production,a well defined scale-up platform is necessary.Recently,novel scenario methods of EB formation in hydrodynamic conditions created by bioreactor culture systems using stirred suspension systems(spinner flasks),rotating cell culture system and rotary orbital culture have allowed large-scale EB formation.Their use allows for continuous monitoring and control of the physical and chemical environment which is difficult to achieve by traditional methods.This review summarizes the current state of production of EBs derived from pluripotent cells in various culture systems.Furthermore,an overview of high quality EB formation strategies coupled with systems for in vitro differentiation into various cell types to be applied in cell replacement therapy is provided in this review. Recently,new insights in induced pluripotent stem(iPS) cell technology showed that differentiation and lineage commitment are not irreversible processes and this has opened new avenues in stem cell research.These cells are equivalent to ES cells in terms of both self-renewal and differentiation capacity.Hence,culture systems for expansion and differentiation of iPS cells can also apply methodologies developed with ES cells,although direct evidence of their use for iPS cells is still limited.
文摘AIM To investigate the impact of medication beliefs,illness perceptions and quality of life on medication adherence in people with decompensated cirrhosis.METHODS One hundred adults with decompensated cirrhosis completed a structured questionnaire when they attended for routine outpatient hepatology review.Measures of self-reported medication adherence(Morisky Medication Adherence Scale),beliefs surrounding medications(Beliefs about Medicines Questionnaire),perceptions of illness and medicines(Brief Illness Perception Questionnaire),and quality of life(Chronic Liver Disease Questionnaire)were examined.Clinical data were obtained via patient history and review of medical records.Least absolute shrinkage and selection operator and stepwise backwards regression techniques were used to construct the multivariable logistic regression model.Statistical significance was set at alpha=0.05.RESULTS Medication adherence was"High"in 42%o f participants,"Medium"in 37%,and"Low"in 21%.Compared to patients with"High"adherence,those with"Medium"or"Low"adherence were more likely to report difficulty affording their medications(P<0.001),lower perception of treatment helpfulness(P=0.003)and stronger medication concerns relative to medication necessity beliefs(P=0.003).People with"Low"adherence also experienced greater symptom burden and poorer quality of life,including more frequent abdominal pain(P=0.023),shortness of breath(P=0.030),and emotional disturbances(P=0.050).Multivariable analysis identified having stronger medication concerns relative to necessity beliefs(Necessity-Concerns Differential≤5,OR=3.66,95%CI:1.18-11.40)and more frequent shortness of breath(shortness of breath score≤3,OR=3.87,95%CI:1.22-12.25)as independent predictors of"Low"adherence.CONCLUSION The association between"Low"adherence and patients having strong concerns or doubting the necessity or helpfulness of their medications should be explored further given the clinical relevance.
文摘This paper presents the main results obtained during a decade of scientific activities in the Meuse/Haute-Marne Underground Research Laboratory （URL） located on the eastern boundary of the Paris Basin, in the Callovo-Oxfordian clay rock formation. The URL was built in the framework of ANDRA＇s research program into the feasibility of a reversible deep geological disposal of high-level and intermediate-level long-lived radioactive （HL, ILLL） waste. Its underground drifts have been used to study a 160-million-year old clay layer. The 2006 Planning Act adopted this disposal concept as the reference solution for the long-term management of HL and ILLL radioactive waste. Today, research is continuing into the design and sitting of the disposal facility which could be commissioned by 2025 if its license is granted in 2016. Through these programs, the laboratory will help ANDRA develop a concrete approach with a view to proposing suitable architectures and management methods for a deep disposal facility, to allow by 2016 the decision for the start of the construction of the shafts and drifts of the new disposal facility.
文摘Within the framework of feasibility studies for a reversible, deep geological repository of high-and intermediate-level long-lived radioactive waste(HLW, IL-LLW), the French National Radioactive Waste Management Agency(Andra) is investigating the Callovo-Oxfordian(COx) formation near Bure(northeast part of France) as a potential host rock for the repository. The hydro-mechanical(HM) behaviour is an important issue to design and optimise different components of the disposal such as shaft, ramp, drift,and waste package disposal facilities. Over the past 20 years, a large number of laboratory experiments have been carried out to characterise and understand the HM behaviours of COx claystones. At the beginning, samples came from deep boreholes drilled at the ground surface with oil base mud. From2000 onwards, with the launch of the construction of the Meuse/Haute-Marne Underground Research Laboratory(MHM URL), most samples have been extracted from a large number of air drilled boreholes in the URL. In parallel, various constitutive models have been developed for modelling. The thermohydro-mechanical(THM) behaviours of the COx claystones were investigated under different repository conditions. Core samples are subjected to a complex HM loading path before testing, due to drilling, conditioning and preparation. Various kinds of effects on the characteristics of the claystones are highlighted and discussed, and the procedures for core extraction and packaging as well as a systematic sample preparation protocol are proposed in order to minimise the uncertainties on test results. The representativeness of the test results is also addressed with regard to the in situ rock mass.
文摘Brown planthopper （Nilaparvata lugens Stal） is one of the most damaging pests causing hopper burn in rice, and thereby reducing the productivity and also the quality of the product. The effective management strategy to control this pest is the identification and transfer of desirable genes to local rice cultivars. The most important approach for developing resistant cultivars is the identification of markers, which can help in marker-assisted selection of more durable resistant genotype. The susceptible parent IR50 and the resistant parent Ptb33, and their F2 populations were used in bulked segregant analysis for identification of resistant genes with random amplified polymorphic DNA marker （RAPD） primers. The primers OPC7 and OPAG14 showed both dominant and susceptible specific banding pattern so called co-dominant markers. Moreover, OPC7697 and OPAG14680 showed resistant specific bands and thus being in coupling phase, whereas OPC7846 and OPAG14650 showed susceptible specific genotypic bands in bulked segregant analysis. Therefore, the coupling phase markers, OPC7697 and OPAG14680, are considered to be more useful in marker-assisted selection of rice genotypes in crop improvement.
文摘The French National Agency for Nuclear Waste Management (Andra) conducted a site investiga- tions program within the project of a deep geological disposal of radioactive waste in the Meuse/ Haute-Marne region. The construction of the tunnel of 5 Km length and the shafts of about 500 m depth to access the repository located in the clay host formation of Callovo-Oxfordian age, will lead to the perturbations of the groundwater flow fields. The prediction of the behaviour of these perturbations is needed to support: 1) the engineering and monitoring operations, and 2) the assessment of the consequences on groundwater resources. A variably-saturated flow model of a local multi-layered aquifer system is developed. It integrates the Oxfordian aquifer (limestone), the Kimmeridgianaquitard (marl) and the Barrois limestone aquifer including the karst conduits network. The variably-saturated flow Richard’s equation is solved with a finite element simulator. Prior to the simulation of the predictive repository impacts, a transient flow model is calibrated with respect to Underground Research Laboratory (URL) construction data. The results are analysed and evaluated by the use of performance measures.
文摘AIM:To compare gene targeting efficiencies,expression profiles,and Ca2+ handling potentials in two widely used mouse embryonic stem cell lines.METHODS:The two widely used mouse embryonic stem cell lines,R1 and HM-1,were cultured and maintained on Mitomycin C treated mouse embryonic fibroblast feeder cell layers,following standard culture procedures.Cells were incubated with primary and secondary antibodies before fluorescence activated cell sorting analysis to compare known pluripotency markers.Moreover,cells were harvested by trypsinization and transfected with a kinase-inactive murine Tyk2 targeting construct,following the BioRad and Amaxa transfection procedures.Subsequently,the cells were cultured and neomycin-resistant cells were picked after 13 d of selection.Surviving clones were screened twice by polymerase chain reaction(PCR) and finally confirmed by Southern blot analysis before comparison.Global gene expression profiles of more than 20 400 probes were also compared and significantly regulated genes were conf irmed by real time PCR analysis.Calcium handling potentials of these cell lines were also compared using various agonists.RESULTS:We found signif icant differences in transfection eff iciencies of the two cell lines(91% ± 6.1% vs 75% ± 4.2%,P = 0.01).Differences in the targeting efficiencies were also significant whether the Amaxa or BioRad platforms were used for comparison.We did not observe signif icant differences in the levels of many known pluripotency markers.However,our genomewide expression analysis using more than 20 400 spotted cDNA arrays identified 55 differentially regulated transcripts(P < 0.05) implicated in various important biological processes,including binding molecular functions(particularly Ca2+ binding roles).Subsequently,we measured Ca2+ signals in these cell lines in response to various calcium agonists,both in high and low Ca2+ solutions,and found signif icant differences(P < 0.05) in the regulation of Ca2+ homeostasis between the investigated cell lines.Then we further compared the detection and expression of various membrane and in-tracellular Ca2+ receptors and similarly found signif icant(P < 0.05) variations in a number of calcium receptors between these cell lines.CONCLUSION:Results of this study emphasize the importance of considering intrinsic cellular variations,during selection of cell lines for experiments and interpretations of experimental results.
文摘Spondylolisthesis is a chronic disease,and a timely diagnosis of it may help in avoiding surgery.Disease identification in x-ray radiographs is very challenging.Strengthening the feature extraction tool in VGG16 has improved the classification rate.But the fully connected layers of VGG16 are not efficient at capturing the positional structure of an object in images.Capsule network(CapsNet)works with capsules(neuron clusters)rather than a single neuron to grasp the properties of the provided image to match the pattern.In this study,an integrated model that is a combination of VGG16 and CapsNet(S-VCNet)is proposed.In the model,VGG16 is used as a feature extractor.After feature extraction,the output is fed to CapsNet for disease identification.A private dataset is used that contains 466 X-ray radiographs,including 186 images displaying a spine with spondylolisthesis and 280 images depicting a normal spine.The suggested model is the first step towards developing a web-based radiological diagnosis tool that can be utilized in outpatient clinics where there are not enough qualified medical professionals.Experimental results demonstrate that the developed model outperformed the other models that are used for lumbar spondylolisthesis diagnosis with 98%accuracy.After the performance check,the model has been successfully deployed on the Gradio web app platform to produce the outcome in less than 20 s.
