The conversion of sound vibration into electrical potential is a critical function performed by cochlear hair cells.Unlike the regenerative capacity found in various other cells throughout the body,cochlear sensory ce...The conversion of sound vibration into electrical potential is a critical function performed by cochlear hair cells.Unlike the regenerative capacity found in various other cells throughout the body,cochlear sensory cells lack the ability to regenerate once damaged.Furthermore,a decline in the quantity of these cells results in a deterioration of auditory function.Piezoelectric materials can generate electric charge in response to sound wave vibration,making them theoretically suitable for replacing hair cell function.This study explores an innovative approach using piezoelectric nanocomposite filaments,namely poly(vinylidene fluoride),poly(vinylidene fluoride)/barium titanate,and poly(vinylidene fluoride)/reduced graphene oxide,as self-powered acoustic sensors designed to function in place of cochlear hair cells.These flexible filaments demonstrate a unique ability to generate electricity in response to frequency sounds from 50 up to 1000 Hz at moderate sound pressure levels(60–95 dB),approaching the audible range with an overall acoustoelectric energy conversion efficiency of 3.25%.Serving as self-powered acoustic sensors,these flexible filaments hold promise for potential applications in cochlear implants,with a high sensitivity of 117.5 mV(Pa-cm^(2))^(-1).The cytocompatibility of these filaments was assessed through in vitro viability tests conducted on three cell lines,serving as a model for inner ear cells.展开更多
Objective:To observe reinforcing effect of calcium sulfate cement(CSC) bovine bone morphogenetic protein(bBMP) on vertebral in the rabbit model of osteoporosis.Methods:A total of 48 New Zealand white rabbits were rand...Objective:To observe reinforcing effect of calcium sulfate cement(CSC) bovine bone morphogenetic protein(bBMP) on vertebral in the rabbit model of osteoporosis.Methods:A total of 48 New Zealand white rabbits were randomly divided into group Ⅰ(blank control group),groupⅡ(CSC injection group),group Ⅲ(CSC/bBMP injection group) and control group.White rabbit osteoporosis model was established rapidly by using castration mcthod+methylprcdnisolone candidate.After modeling,groups Ⅱ.Ⅲ were given corresponding vertebral body injection material,and 4 animals were sacrificed respectively at 24 h,6 weeks,12 weeks after vertebral plasty.Tissue pathological status,vertebral mineral density and vertebral body bone mechanical strength were observed.Results:Vertebral body structure form was normal in the groups Ⅱand Ⅲ.Trabecular bone coarsens,connection and repair were observed in micro fracture and bone defects,bone trabecular connectivity was superior to group I significantly;vertebral body compression strength in the group I was on the decline,vertebral compression strength in the groups Ⅱ and Ⅲ was on the rise,the largest vertebra.Postoperative BMC and BMD in groups Ⅱand Ⅲ were incresed,and significantly higher than group I after 6 weeks(P<0.05),BMC and BMD in group Ⅲ after 12 weeks were higher than the other three groups.Conclusion:Compound bBMP CSC has good bone induction.It can improve the three-dimensional construction effect for osteoporosis vertebral trabecula,and can significantly improve the vertebral strength,as a vertebral packing material with good application prospect.展开更多
The long-term outcomes of 157 patients affected by Peyronie＇s disease （PD） who underwent penile straightening with Egydio＇s technique between January 2004 and December 2008 are reported. Only patients with PD who ...The long-term outcomes of 157 patients affected by Peyronie＇s disease （PD） who underwent penile straightening with Egydio＇s technique between January 2004 and December 2008 are reported. Only patients with PD who were stable for at least 6-12 months prior to surgery were enrolled in this study. Preoperative assessment included a dynamic echo colour Doppler ultrasound scan to evaluate the degree of penile deformity and the peak systolic velocity in the cavernosal arteries and an assessment of erectile function with the administration of the International Index of Erectile Function 5 （IIEF-5） questionnaire. Stretched penile length was recorded pre- and postoperatively. Surgical complications, cosmesis and sexual function, patient satisfaction and postoperative erectile function were assessed postoperatively at 3 months, I year and 2 years, respectively. After a median follow-up period of 20 months （range： 12-24 months）, we found that mild residual curvature （12%） and glans hypoesthesia （3%） were the only causes of partial dissatisfaction. No rejection of the graft was observed. All patients recovered their ability to penetrate with no difficulty. In addition, an intraoperative average increase of 2.5 cm （range： 1.7-4.1 cm） in stretched penile length was recorded, with all patients engaging in penetrative sexual intercourse. In conclusion, this procedure represents a safe and reproducible technique for the correction of penile curvature resulting from PD and yields excellent cosmetic and functional results.展开更多
The cleft palate is a defect of longitudinal fusion that affects the bone and the mucosa in the midline of the hard palate.This defect in the fusion of the palatal lateral shelves from the maxillary processes results ...The cleft palate is a defect of longitudinal fusion that affects the bone and the mucosa in the midline of the hard palate.This defect in the fusion of the palatal lateral shelves from the maxillary processes results in an open cleft between the oral and nasal cavities,also known as secondary palatine cleft.The objective of this work is to report the morphological characteristics of palatine clefts observed in stillborn dogs and neonates submitted to euthanasia.Six dogs were evaluated,from each animal the following data were obtained:clinical history,weight and morphometric data.Externally,data regarding to:sex,fur color,malformations,and evaluations of natural openings were evaluated.Every evaluated animal was delivered by caesarean section,50%of stillborn infants had cleft lip associated with secondary cleft palate,50%were female and only one stillborn was a mongrel dog.All others were from the brachycephalic races.It was observed that the cleft palate was found,in great majority,in females,proving the association of sex with this congenital anomaly.In this sense,clinical inspection,including physical examination and anamnesis should be part of the assessment of the neonate patient as parameters to reach a diagnosis and,when it is possible,to be associated with a radiographic image as a complementary method,being the radiography an important auxiliary exam in this context.展开更多
Using a rabbit model leading to myositis in response to exercise-induced muscle overuse, we have previously observed that TNF-alpha is involved in the exercised muscle in early developing myositis as well as both ipsi...Using a rabbit model leading to myositis in response to exercise-induced muscle overuse, we have previously observed that TNF-alpha is involved in the exercised muscle in early developing myositis as well as both ipsiand contralaterally in the myositis which develops in response to a lengthened period of overuse. It is unknown if TNF-alpha can also be engaged contralaterally in early stages of myositis. The hypothesis was that this is the case. It was therefore evaluated whether the TNF-alpha system is early involved contralaterally. An experimental model of 1 week of overuse of the soleus muscle on one side leading to myositis was used, and in situ hybridization and immunohistochemistry were applied to study the expression patterns of TNF-alpha in the soleus muscle in the contralateral side. TNF-alpha was expressed in the myositis process which occurred contralaterally. There were thus TNF-alpha mRNA reactions in the cells of the inflammatory infiltrates, in blood vessel walls and in certain of the muscle fibers. Parts of the latter were necrotic fibers, whereas others were interpreted to be in a regenerative stage. TNF-alpha immunoreactions were seen for infiltrating white blood cells. The observations show that the TNF-alpha system is early involved in the cross-over effects that occur in response to unilateral muscle overuse leading to myositis bilaterally. TNF-alpha is likely to have pro-inflammatory and destructive effects but also to have effects in the muscle regenerative processes. The occurrence of an early involvement of the TNF-alpha system contralaterally to the injury side shows a new aspect of importance of this system in inflammation.展开更多
基金financial support received from Deakin University through the Alfred Deakin Postdoctoral Research Fellowship(2022)partially funded by the European Union–Next-Generation EU via the Italian Ministry of University and Research(MUR),PRIN 2022 Program(PROMISE project,CUP 153D23004700006)+1 种基金support from the Australian National Fabrication Facility(ANFF)the ARC Research Hub for Future Fibres.
文摘The conversion of sound vibration into electrical potential is a critical function performed by cochlear hair cells.Unlike the regenerative capacity found in various other cells throughout the body,cochlear sensory cells lack the ability to regenerate once damaged.Furthermore,a decline in the quantity of these cells results in a deterioration of auditory function.Piezoelectric materials can generate electric charge in response to sound wave vibration,making them theoretically suitable for replacing hair cell function.This study explores an innovative approach using piezoelectric nanocomposite filaments,namely poly(vinylidene fluoride),poly(vinylidene fluoride)/barium titanate,and poly(vinylidene fluoride)/reduced graphene oxide,as self-powered acoustic sensors designed to function in place of cochlear hair cells.These flexible filaments demonstrate a unique ability to generate electricity in response to frequency sounds from 50 up to 1000 Hz at moderate sound pressure levels(60–95 dB),approaching the audible range with an overall acoustoelectric energy conversion efficiency of 3.25%.Serving as self-powered acoustic sensors,these flexible filaments hold promise for potential applications in cochlear implants,with a high sensitivity of 117.5 mV(Pa-cm^(2))^(-1).The cytocompatibility of these filaments was assessed through in vitro viability tests conducted on three cell lines,serving as a model for inner ear cells.
基金supported by Scientific Research Project of People's Hospital number IN Xinjiang Uygur Autonomous Region(20130255)
文摘Objective:To observe reinforcing effect of calcium sulfate cement(CSC) bovine bone morphogenetic protein(bBMP) on vertebral in the rabbit model of osteoporosis.Methods:A total of 48 New Zealand white rabbits were randomly divided into group Ⅰ(blank control group),groupⅡ(CSC injection group),group Ⅲ(CSC/bBMP injection group) and control group.White rabbit osteoporosis model was established rapidly by using castration mcthod+methylprcdnisolone candidate.After modeling,groups Ⅱ.Ⅲ were given corresponding vertebral body injection material,and 4 animals were sacrificed respectively at 24 h,6 weeks,12 weeks after vertebral plasty.Tissue pathological status,vertebral mineral density and vertebral body bone mechanical strength were observed.Results:Vertebral body structure form was normal in the groups Ⅱand Ⅲ.Trabecular bone coarsens,connection and repair were observed in micro fracture and bone defects,bone trabecular connectivity was superior to group I significantly;vertebral body compression strength in the group I was on the decline,vertebral compression strength in the groups Ⅱ and Ⅲ was on the rise,the largest vertebra.Postoperative BMC and BMD in groups Ⅱand Ⅲ were incresed,and significantly higher than group I after 6 weeks(P<0.05),BMC and BMD in group Ⅲ after 12 weeks were higher than the other three groups.Conclusion:Compound bBMP CSC has good bone induction.It can improve the three-dimensional construction effect for osteoporosis vertebral trabecula,and can significantly improve the vertebral strength,as a vertebral packing material with good application prospect.
文摘The long-term outcomes of 157 patients affected by Peyronie＇s disease （PD） who underwent penile straightening with Egydio＇s technique between January 2004 and December 2008 are reported. Only patients with PD who were stable for at least 6-12 months prior to surgery were enrolled in this study. Preoperative assessment included a dynamic echo colour Doppler ultrasound scan to evaluate the degree of penile deformity and the peak systolic velocity in the cavernosal arteries and an assessment of erectile function with the administration of the International Index of Erectile Function 5 （IIEF-5） questionnaire. Stretched penile length was recorded pre- and postoperatively. Surgical complications, cosmesis and sexual function, patient satisfaction and postoperative erectile function were assessed postoperatively at 3 months, I year and 2 years, respectively. After a median follow-up period of 20 months （range： 12-24 months）, we found that mild residual curvature （12%） and glans hypoesthesia （3%） were the only causes of partial dissatisfaction. No rejection of the graft was observed. All patients recovered their ability to penetrate with no difficulty. In addition, an intraoperative average increase of 2.5 cm （range： 1.7-4.1 cm） in stretched penile length was recorded, with all patients engaging in penetrative sexual intercourse. In conclusion, this procedure represents a safe and reproducible technique for the correction of penile curvature resulting from PD and yields excellent cosmetic and functional results.
文摘The cleft palate is a defect of longitudinal fusion that affects the bone and the mucosa in the midline of the hard palate.This defect in the fusion of the palatal lateral shelves from the maxillary processes results in an open cleft between the oral and nasal cavities,also known as secondary palatine cleft.The objective of this work is to report the morphological characteristics of palatine clefts observed in stillborn dogs and neonates submitted to euthanasia.Six dogs were evaluated,from each animal the following data were obtained:clinical history,weight and morphometric data.Externally,data regarding to:sex,fur color,malformations,and evaluations of natural openings were evaluated.Every evaluated animal was delivered by caesarean section,50%of stillborn infants had cleft lip associated with secondary cleft palate,50%were female and only one stillborn was a mongrel dog.All others were from the brachycephalic races.It was observed that the cleft palate was found,in great majority,in females,proving the association of sex with this congenital anomaly.In this sense,clinical inspection,including physical examination and anamnesis should be part of the assessment of the neonate patient as parameters to reach a diagnosis and,when it is possible,to be associated with a radiographic image as a complementary method,being the radiography an important auxiliary exam in this context.
基金Financial support has been obtained from the Faculty of Medicine,Umea University,and the J.C.Kempe and Seth M.Kempe Memorial Foundations,Ornskoldsvik
文摘Using a rabbit model leading to myositis in response to exercise-induced muscle overuse, we have previously observed that TNF-alpha is involved in the exercised muscle in early developing myositis as well as both ipsiand contralaterally in the myositis which develops in response to a lengthened period of overuse. It is unknown if TNF-alpha can also be engaged contralaterally in early stages of myositis. The hypothesis was that this is the case. It was therefore evaluated whether the TNF-alpha system is early involved contralaterally. An experimental model of 1 week of overuse of the soleus muscle on one side leading to myositis was used, and in situ hybridization and immunohistochemistry were applied to study the expression patterns of TNF-alpha in the soleus muscle in the contralateral side. TNF-alpha was expressed in the myositis process which occurred contralaterally. There were thus TNF-alpha mRNA reactions in the cells of the inflammatory infiltrates, in blood vessel walls and in certain of the muscle fibers. Parts of the latter were necrotic fibers, whereas others were interpreted to be in a regenerative stage. TNF-alpha immunoreactions were seen for infiltrating white blood cells. The observations show that the TNF-alpha system is early involved in the cross-over effects that occur in response to unilateral muscle overuse leading to myositis bilaterally. TNF-alpha is likely to have pro-inflammatory and destructive effects but also to have effects in the muscle regenerative processes. The occurrence of an early involvement of the TNF-alpha system contralaterally to the injury side shows a new aspect of importance of this system in inflammation.
