The extraction of anthocyanins present in the skin of the dragon fruit was performed using trifluoroacetic acid (TFA) plus a mixture of methanol, acetic acid and water;the anthocyanins were then purified with a LC-18 ...The extraction of anthocyanins present in the skin of the dragon fruit was performed using trifluoroacetic acid (TFA) plus a mixture of methanol, acetic acid and water;the anthocyanins were then purified with a LC-18 cartridge, using methanol acidified with TFA as eluent, reaching concentrations of 44.3865 ± 1.3125 mg/100g of sample. The extracts were put through stability tests under different storage conditions, modifying the pH of the extracts (pH of 1, 4 and 6), the temperature (4℃, 25℃ and 68℃) and the absence and presence of light for a time period of 4 days;the tests indiated that anthocyanins remain more stable at a temperature of 4℃?with a pH of 4 in the absence of light, retaining up to 80% of the pigment. Three anthocyanins were partially identified in the extracts by high performance liquid chromatography (HPLC);they were: cyanidin 3-O-glucoside, cyanidin 3,5 O-glucoside and pelargonidin 3,5 O-glucoside.展开更多
The browning of fruits can be considered as an enzymatic oxidation which is believed to be one of the main causes of quality loss during post-harvest handling. The enzymes responsible for this are the oxidoreductases;...The browning of fruits can be considered as an enzymatic oxidation which is believed to be one of the main causes of quality loss during post-harvest handling. The enzymes responsible for this are the oxidoreductases;the polyphenol oxidase (PPO) (monophenol, o-diphenol, oxygen oxidoreductase;EC 1.14.18.1) belongs to this group. This enzyme, which is found in the sapodilla plum (Achras sapota), was purified using a phenylsepharose and a SephacrylS-200 columns. The molecular weight of the purified enzyme was estimated to be about 66 kDa by gel filtration and 29 kDa by SDS-PAGE. A single protein band was found using the latter system (SDS-PAGE), which shows that the PPO of the pulp of the sapodilla plum may be composed of two protein subunits with similar molecular weight. The optimum pH was 7.0 and the optimum temperature 60℃. The most effective inhibitors tested were ascorbic acid, sodium metabisulfite and acetic acid.展开更多
The growing development of biological products highlights the social and environmental responsibility that several industrial companies are facing in recent years.In this context,the advancement of bioprocessing as an...The growing development of biological products highlights the social and environmental responsibility that several industrial companies are facing in recent years.In this context,the advancement of bioprocessing as an alternative for exploring the potential of ecologically based products,especially in biofuels,food,and agro-industrial business,exposes the rational efficiency of the application of renewable sources in different industrial segments.Industries strongly associated with food production concentrate large amounts of wastes rich in bioactive compounds.A range of highly effective technologies has been highly explored to recover large concentrations of prominent compounds present in these materials.The advances in this scenario assurance value addition to these by-products,in addition to highlighting their various technological applications,considering the biorefinery and ecologically based production concepts.Accordingly,this review article described a detailed and systematic approach to the importance of using bioactive compounds and exploring the main sources of these elements.Also,some recent and innovative research that has achieved encouraging results was highlighted.Furthermore,the study included the main extraction technologies that have been investigated as a strategy of prospecting the application of bioactive compounds and optimizing the processes for obtaining natural compounds from plant sources.Finally,future outlooks were presented to contribute to the innovative opportunities and applicability of highly promising technologies and manipulations of bioactive compounds from a range of perspectives.展开更多
In order to take advantage of the lignocellulosic material in water hyacinth （Eichhornia crassipes）, dehydration pretreatment in the first step and then sodium hydroxide and hydrogen peroxide pretreatment was perfor...In order to take advantage of the lignocellulosic material in water hyacinth （Eichhornia crassipes）, dehydration pretreatment in the first step and then sodium hydroxide and hydrogen peroxide pretreatment was performed. The microorganism used for the fermentation process was Zimomonas mobilis. Batch fermentation experiments were carried out with four tests using 22 factorial design with two levels leadings to evaluate the effect of NaOH concentration, conditioning salts as independent variables and ethanol produced as a dependent variable. The optimum condition with higher amount of glucose hydrolyzed and ethanol was： substrate conditioning cellulases, it was pretreated 10% NaOH, with 92.38% conversion of glucose to ethanol and yield of 0.47 g ethanol per g of glucose and 0.018 g ethanol per g of biomass.展开更多
We developed and implemented a methodology that allowed extracting and evaluating high molecular weight polysaccharides present in the gel of Aloe barbadensis Miller. One of the fractions evaluated revealed the presen...We developed and implemented a methodology that allowed extracting and evaluating high molecular weight polysaccharides present in the gel of Aloe barbadensis Miller. One of the fractions evaluated revealed the presence of high molecular weight carbohydrates (200 kDa) with a behavior similar to that of acemannan and another fraction with compounds of molecular weights between 17 and 47 kDa. We quantified the concentration of acemannan for two different growing periods. The concentration of acemannan in the high molecular weight fraction was 99.97 ppm in the rainy season and 106.03 ppm in the dry season. The concentration of acemannan in the fraction of low molecular weight was 9.364 ppm during the season of greatest rainfall and 26.939 ppm in the dry season.展开更多
Aloe vera (Aloe barbadensis Miller) possesses curative and therapeutic properties attributed to the polysaccharides found in its tissue. This work aimed to study hydrolyzed (chemical and enzymatical) Aloe vera gel ext...Aloe vera (Aloe barbadensis Miller) possesses curative and therapeutic properties attributed to the polysaccharides found in its tissue. This work aimed to study hydrolyzed (chemical and enzymatical) Aloe vera gel extracts using ultrafiltration membranes with a pore size of 0.22 micron (μm). Nine chemical treatments were achieved with H2SO4 and three wet heat treatments were carried out at a pressure of 1.2 lbf/in2 for 15 minutes;in both cases, three different concentrations of Aloe vera gel juice (AGJ) were used: 1.5%, 2.5% and 3.5% w/w. The concentrations of H2SO4 were 0.10%, 0.25% and 0.40% w/w. Chemical experiments are performed over a factorial 32 design and results were analyzed using SPSS software (version 17, SPSS Inc.), finding the one labeled T7 (15 ml of AGJ 3.5% with 15 ml of H2SO4 0.10% added) the best of them, as it leaded to 0.0446 mg/ml of liberated glucose. Among the three wet heat treatments, the one labeled TC3 (15 ml of AGJ 3.5% with 15 ml of H2O added) was the best-performing one, as it leaded to 0.292 mg/ml of liberated glucose. Furthermore, an enzymatic hydrolysis was carried out using Novozymes’ Pectinex? AR and Viscozyme?. Hydrolisis with both enzymes yield to better results than acid hydrolysis: in the treatment with Pectinex? AR, 3.282 mg/ml of liberated glucose were obtained and 3.302 mg/ml in the treatment with Viscozyme?. The hydrolyzed substances obtained by acid and enzymatic hydrolysis, as well as by wet heat treatment, were subsequently analyzed by thin layer chromatography (TLC), using glucose, galactose and arabinose 1000 ppm solutions as reference patterns. Among the treatments by H2SO4, the one labeled T4 obtained an Rf value of 50, the same as on the galactose reference pattern.展开更多
In recent decades,third-generation(3G)biofuels have become a more attractive method of fuel production,as algae cultivation does not infringe on resources needed for food production.Additionally,algae can adapt to dif...In recent decades,third-generation(3G)biofuels have become a more attractive method of fuel production,as algae cultivation does not infringe on resources needed for food production.Additionally,algae can adapt to different environments,has high photosynthetic efficiency(CO_(2)fixation),and has a high potential for carbohydrate accumulation.The prevalence of algae worldwide demonstrates its ability to adapt to different environments and climates,proving its biodiversity and versatility.Algae can be grown in wastewater,seawater,and even sewage,thus ensuring a lower water footprint and greater energy efficiency during algal biomass production.Because of this,the optimization of 3G ethanol production appears to be an excellent alternative to mitigate environmental impacts and increase energy and food security.This critical review presents(i)the stages of cultivation and processing of micro and macroalgae;(ii)the selection of yeasts(through engineering and/or bioprospecting)to produce ethanol from these biomasses;(iii)the potential of seawater-based facilities to reduce water footprint;and(iv)the mass and energy balances of 3G ethanol production in the world energy matrix.This article is,above all,a brainstorm on the environmental viability of algae bioethanol.展开更多
文摘The extraction of anthocyanins present in the skin of the dragon fruit was performed using trifluoroacetic acid (TFA) plus a mixture of methanol, acetic acid and water;the anthocyanins were then purified with a LC-18 cartridge, using methanol acidified with TFA as eluent, reaching concentrations of 44.3865 ± 1.3125 mg/100g of sample. The extracts were put through stability tests under different storage conditions, modifying the pH of the extracts (pH of 1, 4 and 6), the temperature (4℃, 25℃ and 68℃) and the absence and presence of light for a time period of 4 days;the tests indiated that anthocyanins remain more stable at a temperature of 4℃?with a pH of 4 in the absence of light, retaining up to 80% of the pigment. Three anthocyanins were partially identified in the extracts by high performance liquid chromatography (HPLC);they were: cyanidin 3-O-glucoside, cyanidin 3,5 O-glucoside and pelargonidin 3,5 O-glucoside.
文摘The browning of fruits can be considered as an enzymatic oxidation which is believed to be one of the main causes of quality loss during post-harvest handling. The enzymes responsible for this are the oxidoreductases;the polyphenol oxidase (PPO) (monophenol, o-diphenol, oxygen oxidoreductase;EC 1.14.18.1) belongs to this group. This enzyme, which is found in the sapodilla plum (Achras sapota), was purified using a phenylsepharose and a SephacrylS-200 columns. The molecular weight of the purified enzyme was estimated to be about 66 kDa by gel filtration and 29 kDa by SDS-PAGE. A single protein band was found using the latter system (SDS-PAGE), which shows that the PPO of the pulp of the sapodilla plum may be composed of two protein subunits with similar molecular weight. The optimum pH was 7.0 and the optimum temperature 60℃. The most effective inhibitors tested were ascorbic acid, sodium metabisulfite and acetic acid.
基金the Coordination for the Improvement of Higher Education Personnel(CAPES)the National Council of Technological and Scientific Development(CNPq)the Research Support Foundation of the State of Rio Grande do Sul(FAPERGS).
文摘The growing development of biological products highlights the social and environmental responsibility that several industrial companies are facing in recent years.In this context,the advancement of bioprocessing as an alternative for exploring the potential of ecologically based products,especially in biofuels,food,and agro-industrial business,exposes the rational efficiency of the application of renewable sources in different industrial segments.Industries strongly associated with food production concentrate large amounts of wastes rich in bioactive compounds.A range of highly effective technologies has been highly explored to recover large concentrations of prominent compounds present in these materials.The advances in this scenario assurance value addition to these by-products,in addition to highlighting their various technological applications,considering the biorefinery and ecologically based production concepts.Accordingly,this review article described a detailed and systematic approach to the importance of using bioactive compounds and exploring the main sources of these elements.Also,some recent and innovative research that has achieved encouraging results was highlighted.Furthermore,the study included the main extraction technologies that have been investigated as a strategy of prospecting the application of bioactive compounds and optimizing the processes for obtaining natural compounds from plant sources.Finally,future outlooks were presented to contribute to the innovative opportunities and applicability of highly promising technologies and manipulations of bioactive compounds from a range of perspectives.
文摘In order to take advantage of the lignocellulosic material in water hyacinth （Eichhornia crassipes）, dehydration pretreatment in the first step and then sodium hydroxide and hydrogen peroxide pretreatment was performed. The microorganism used for the fermentation process was Zimomonas mobilis. Batch fermentation experiments were carried out with four tests using 22 factorial design with two levels leadings to evaluate the effect of NaOH concentration, conditioning salts as independent variables and ethanol produced as a dependent variable. The optimum condition with higher amount of glucose hydrolyzed and ethanol was： substrate conditioning cellulases, it was pretreated 10% NaOH, with 92.38% conversion of glucose to ethanol and yield of 0.47 g ethanol per g of glucose and 0.018 g ethanol per g of biomass.
文摘We developed and implemented a methodology that allowed extracting and evaluating high molecular weight polysaccharides present in the gel of Aloe barbadensis Miller. One of the fractions evaluated revealed the presence of high molecular weight carbohydrates (200 kDa) with a behavior similar to that of acemannan and another fraction with compounds of molecular weights between 17 and 47 kDa. We quantified the concentration of acemannan for two different growing periods. The concentration of acemannan in the high molecular weight fraction was 99.97 ppm in the rainy season and 106.03 ppm in the dry season. The concentration of acemannan in the fraction of low molecular weight was 9.364 ppm during the season of greatest rainfall and 26.939 ppm in the dry season.
文摘Aloe vera (Aloe barbadensis Miller) possesses curative and therapeutic properties attributed to the polysaccharides found in its tissue. This work aimed to study hydrolyzed (chemical and enzymatical) Aloe vera gel extracts using ultrafiltration membranes with a pore size of 0.22 micron (μm). Nine chemical treatments were achieved with H2SO4 and three wet heat treatments were carried out at a pressure of 1.2 lbf/in2 for 15 minutes;in both cases, three different concentrations of Aloe vera gel juice (AGJ) were used: 1.5%, 2.5% and 3.5% w/w. The concentrations of H2SO4 were 0.10%, 0.25% and 0.40% w/w. Chemical experiments are performed over a factorial 32 design and results were analyzed using SPSS software (version 17, SPSS Inc.), finding the one labeled T7 (15 ml of AGJ 3.5% with 15 ml of H2SO4 0.10% added) the best of them, as it leaded to 0.0446 mg/ml of liberated glucose. Among the three wet heat treatments, the one labeled TC3 (15 ml of AGJ 3.5% with 15 ml of H2O added) was the best-performing one, as it leaded to 0.292 mg/ml of liberated glucose. Furthermore, an enzymatic hydrolysis was carried out using Novozymes’ Pectinex? AR and Viscozyme?. Hydrolisis with both enzymes yield to better results than acid hydrolysis: in the treatment with Pectinex? AR, 3.282 mg/ml of liberated glucose were obtained and 3.302 mg/ml in the treatment with Viscozyme?. The hydrolyzed substances obtained by acid and enzymatic hydrolysis, as well as by wet heat treatment, were subsequently analyzed by thin layer chromatography (TLC), using glucose, galactose and arabinose 1000 ppm solutions as reference patterns. Among the treatments by H2SO4, the one labeled T4 obtained an Rf value of 50, the same as on the galactose reference pattern.
基金the Coordination for the Improvement of Higher Education Personnel(CAPES)the National Council of Technological and Scientific Development(CNPq)and the Research Support Foundation of the State of Rio Grande do Sul(FAPERGS,16/2551-0000522-2,17/2551-0000893-6,19/2551-0001261-6)for the financial support+3 种基金C.Müller thanks CAPES/PNPD(88887.352933/2019-00)for a postdoctoral fellowship.M.V.Tres(308936/2017-5)G.L.Zabot(304882/2018-6)H.Treichel(305258/2018-4)L.M.Colla(305935/2017-8)thank CNPq for the productivity grants.
文摘In recent decades,third-generation(3G)biofuels have become a more attractive method of fuel production,as algae cultivation does not infringe on resources needed for food production.Additionally,algae can adapt to different environments,has high photosynthetic efficiency(CO_(2)fixation),and has a high potential for carbohydrate accumulation.The prevalence of algae worldwide demonstrates its ability to adapt to different environments and climates,proving its biodiversity and versatility.Algae can be grown in wastewater,seawater,and even sewage,thus ensuring a lower water footprint and greater energy efficiency during algal biomass production.Because of this,the optimization of 3G ethanol production appears to be an excellent alternative to mitigate environmental impacts and increase energy and food security.This critical review presents(i)the stages of cultivation and processing of micro and macroalgae;(ii)the selection of yeasts(through engineering and/or bioprospecting)to produce ethanol from these biomasses;(iii)the potential of seawater-based facilities to reduce water footprint;and(iv)the mass and energy balances of 3G ethanol production in the world energy matrix.This article is,above all,a brainstorm on the environmental viability of algae bioethanol.
