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TLR7激动剂对P.y 17XNL感染BALB/c小鼠免疫调节效应的影响 被引量:2
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作者 邱悦 赵永红 曹雅明 《微生物学杂志》 CAS CSCD 2019年第4期71-75,共5页
宿主抗疟保护性免疫应答的水平和强度与疟疾预后关系密切,当疟原虫侵袭宿主时,TLRs向机体传达病原体入侵信息,激活免疫系统。采用TLR7激动剂处理P.y 17XNL感染的BALB/c小鼠,通过FACS和ELISA检测DC亚群(mDC和pDC)、CD4^+ T细胞亚群(Th1、... 宿主抗疟保护性免疫应答的水平和强度与疟疾预后关系密切,当疟原虫侵袭宿主时,TLRs向机体传达病原体入侵信息,激活免疫系统。采用TLR7激动剂处理P.y 17XNL感染的BALB/c小鼠,通过FACS和ELISA检测DC亚群(mDC和pDC)、CD4^+ T细胞亚群(Th1、Th2、Tfh和Treg)和细胞因子(IFN-γ、IL-4、IL-21和IL-10)的水平,明确TLR7通过DC调控宿主应答的免疫机制。结果显示,TLR7激动剂能够促进DC亚群数量的增加,同时也提高Th1和Tfh细胞分化,并显著增加IFN-γ分泌水平。因此,TLR7激动剂通过诱导DC活化,促进Th1型免疫应答降低原虫血症水平,在疟疾感染过程中发挥保护性免疫作用。 展开更多
关键词 疟疾 TOLL样受体7 T细胞亚群 树突状细胞
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CVB对OL细胞和OL/BDV细胞I型IFN信号转导途径的影响
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作者 翟爱霞 颜冬梅 +4 位作者 王海萍 卜桐 崔乐乐 考文萍 张凤民 《微生物学杂志》 CAS CSCD 2019年第4期46-51,共6页
探讨柯萨奇病毒(coxsackievirus, CVB)对人类少突胶质细胞(oligodendrocyte, OL)和博尔纳病病毒(Borna disease virus, BDV)持续感染的OL细胞(OL/BDV)中I型干扰素(interferon, IFN)、microRNA-155(miR-155)表达以及干扰素调节因子(inter... 探讨柯萨奇病毒(coxsackievirus, CVB)对人类少突胶质细胞(oligodendrocyte, OL)和博尔纳病病毒(Borna disease virus, BDV)持续感染的OL细胞(OL/BDV)中I型干扰素(interferon, IFN)、microRNA-155(miR-155)表达以及干扰素调节因子(interferon regulatory factor, IRF)7细胞定位的影响。CVB感染OL细胞和OL/BDV细胞0、2、4、6、12和24 h,qPCR检测I型IFN mRNA和miR-155表达水平。OL细胞和OL/BDV细胞分别转染IRF7-EGFP质粒,CVB感染4 h,观察IRF7细胞定位情况。CVB感染OL细胞2、4、12和24 h,IFN-αmRNA和miR-155表达水平显著增加,IFN-βmRNA表达水平在4、12和24 h显著增加;CVB感染OL/BDV细胞IFN-α和IFN-βmRNA表达水平除0 h均显著增加,但miR-155表达仅在12和24 h显著增加。CVB感染OL细胞和OL/BDV细胞4 h,IRF7绿色荧光蛋白由细胞浆转移至细胞核内。综上所述,CVB可诱导OL细胞和OL/BDV细胞I型IFN、miR-155的表达,促进IRF7入核,从而激活I型IFN信号转导途径。 展开更多
关键词 柯萨奇病毒 OL细胞 OL/BDV细胞 I型IFN信号转导途径 microRNA-155
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耐碳青霉烯类鲍曼不动杆菌同源性及生物膜形成能力分析 被引量:7
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作者 赵永鑫 王晓红 +5 位作者 王英 郭宇航 范学财 王勇 包名家 张晓丽 《微生物学杂志》 CAS CSCD 2019年第4期65-70,共6页
分析耐碳青霉烯类鲍曼不动杆菌(Carbapenem Resistant Acinetobacter baumannii,CRAB)的同源性,测定其生物膜形成能力,分析blaOXA基因携带与生物膜形成能力的相关性,探讨CRAB在医院暴发的影响因素。收集佳木斯大学附属第一医院非重复CRA... 分析耐碳青霉烯类鲍曼不动杆菌(Carbapenem Resistant Acinetobacter baumannii,CRAB)的同源性,测定其生物膜形成能力,分析blaOXA基因携带与生物膜形成能力的相关性,探讨CRAB在医院暴发的影响因素。收集佳木斯大学附属第一医院非重复CRAB共35株,全自动微生物鉴定系统VITEK-II及体外敏感实验筛选CRAB,多序列位点分型(MLST)、肠道细菌基因重复序列聚合酶链式反应(ERIC-PCR)分析其同源性,结晶紫染色法定量检测生物膜形成能力。33株CRAB为ST 2型,1株ST 671型,1株ST 1199型为新发现的MLST分型。ERIC可分为A、B、C、D四型,A型9株,B型24株,C型1株,D型1株。CRAB及碳青霉烯类敏感鲍曼不动杆菌(CSAB)组生物膜形成能力差异有统计学意义(0.330±0.258、0.534±0.402,P<0.05);blaOXA-23、blaOXA-51、blaOXA-24基因携带与CRAB生物膜形成能力无明确相关性(P>0.05)。生物膜形成能力与碳青霉烯类耐药性的获得呈现负相关。ERIC-PCR分辨率较强,且与MLST具有一定相关性。ST2型为主要流行菌株,患者的高危因素以及医护人员的消毒意识可能与CRAB的暴发有关。 展开更多
关键词 碳青霉烯类耐药 生物膜 同源性分析
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艰难梭菌研究现状 被引量:5
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作者 肖艳艳 张智洁 秦晓松 《微生物学杂志》 CAS CSCD 2018年第2期105-113,共9页
艰难梭菌(Clostridium difficile,CD)是产芽胞革兰阳性厌氧菌,产毒素艰难梭菌可导致艰难梭菌感染(Clostridium difficile infection,CDI)。产毒素艰难梭菌已被公认是抗菌药物相关性腹泻最常见的病原体,也是发达国家最常见的导致住院患... 艰难梭菌(Clostridium difficile,CD)是产芽胞革兰阳性厌氧菌,产毒素艰难梭菌可导致艰难梭菌感染(Clostridium difficile infection,CDI)。产毒素艰难梭菌已被公认是抗菌药物相关性腹泻最常见的病原体,也是发达国家最常见的导致住院患者腹泻感染的原因之一。高毒力菌株导致全世界范围内CDI的发病率和死亡率增加。如何快速而准确地检测CD一直是备受关注的问题,国内外多推荐两步法和三步法进行检测。艰难梭菌治疗的一线用药是甲硝唑、万古霉素以及非达霉素,应用粪便微生物移植(faecal microbiota transplantation,FMT)也可作为治疗轻度或严重复发CDI(recurrence Clostridium difficile infection,rCDI)的可选方法。艰难梭菌的芽胞能抵抗不良环境,但抗菌药物的使用,医院环境的复杂性和医务工作者的手等因素会导致艰难梭菌易于传播,因此医疗机构应加强艰难梭菌的预防。本文主要对艰难梭菌的流行病学、致病机制、检测及治疗进行综述。 展开更多
关键词 艰难梭菌 艰难梭菌感染 两步法 粪便微生物移植
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兔结核病模型的应用研究进展 被引量:2
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作者 涂振阳 蓝常贡 +4 位作者 谢克恭 陆敏安 农峰 梁俊卿 唐盛斐 《微生物学杂志》 CAS CSCD 2019年第6期102-108,共7页
结核病(tuberculosis,TB)由结核分枝杆菌(Mycobacterium tuberculosis,MTB)感染所致,是严重危害患者身心健康的慢性传染病。目前TB的发病机制尚未完全阐明。因此建立恰当的动物模型有助于进一步探索TB发病机制、研发全新高效疫苗,优化... 结核病(tuberculosis,TB)由结核分枝杆菌(Mycobacterium tuberculosis,MTB)感染所致,是严重危害患者身心健康的慢性传染病。目前TB的发病机制尚未完全阐明。因此建立恰当的动物模型有助于进一步探索TB发病机制、研发全新高效疫苗,优化治疗方案提供可靠的实验和理论依据。兔TB模型是应用较为广泛的动物模型之一。综述了兔TB模型构建特点及应用进展,从免疫机制和可行性角度深入分析兔TB模型的优势及其局限性,同时梳理了近年来国内外通过构建兔TB模型用于药物研究、疫苗研发和TB治疗方案评价等方面发挥的作用,为临床研究提供参考。 展开更多
关键词 动物模型 结核病 结核分枝杆菌
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EGCG通过PI3-K/Akt信号通路诱导人甲状腺乳头状癌细胞K1增殖和凋亡的影响 被引量:3
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作者 赵星智 胡新华 《微生物学杂志》 CAS CSCD 2019年第3期64-70,共7页
研究表没食子儿茶素没食子酸酯(EGCG)通过PI3-K/Akt信号通路对人甲状腺乳头状癌细胞K1增殖和凋亡的影响。利用MTT法研究不同剂量EGCG对K1细胞的增殖作用;采用流式细胞术分析EGCG对K1细胞周期和凋亡影响;Westernblot方法检测分析EGCG对... 研究表没食子儿茶素没食子酸酯(EGCG)通过PI3-K/Akt信号通路对人甲状腺乳头状癌细胞K1增殖和凋亡的影响。利用MTT法研究不同剂量EGCG对K1细胞的增殖作用;采用流式细胞术分析EGCG对K1细胞周期和凋亡影响;Westernblot方法检测分析EGCG对人甲状腺乳头状癌K1细胞PI3-K、Akt/p-Akt、mTOR、cyclinD1、CDK4、Bcl-2/Bad、cleaved-caspase-3蛋白表达影响。MTT结果显示EGCG作用后K1细胞增殖显著受到抑制,且表现出明显的剂量依赖性和时间依赖性(P<0.001);流式细胞术结果显示EGCG能够将K1细胞阻滞于G1期,并且产生剂量依赖性诱导K1细胞凋亡(P<0.001);Westernblot结果显示EGCG能够上调K1细胞促凋亡蛋白Bad及cleaved-caspase-3的表达,下调PI3-K、mTOR、cyclinD1、CDK4蛋白表达,降低AKT蛋白磷酸化及抑凋亡蛋白Bcl-2表达(P<0.05)。结果证明,EGCG能够通过PI3-K/Akt信号通路,诱导G1阻滞及细胞凋亡,抑制人甲状腺乳头状癌细胞K1增殖。 展开更多
关键词 PI3-K/AKT通路 表没食子儿茶素没食子酸酯 周期阻滞 细胞凋亡
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IL-21/IL-21R信号在TNBS诱导小鼠炎症性肠病病变形成中的作用研究 被引量:1
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作者 姜雪峰 贾楠 +3 位作者 岳丹 王媛媛 张晓清 孙逊 《微生物学杂志》 CAS CSCD 2019年第1期77-83,共7页
探讨IL-21/IL21R信号在炎症性肠病病变形成中对肠固有层辅助T细胞应答的调控机制。利用IL-21R基因敲除小鼠(IL-21R KO),建立TNBS诱导的炎症性肠病动物模型。监测小鼠体重及生存率;HE染色观察小鼠肠组织形态结构及炎症反应情况;分离和提... 探讨IL-21/IL21R信号在炎症性肠病病变形成中对肠固有层辅助T细胞应答的调控机制。利用IL-21R基因敲除小鼠(IL-21R KO),建立TNBS诱导的炎症性肠病动物模型。监测小鼠体重及生存率;HE染色观察小鼠肠组织形态结构及炎症反应情况;分离和提取肠固有层淋巴细胞(lamina propria lymphocyte,LPL),应用流式细胞仪检测Th1、Th2、Th17、Treg细胞比例和绝对数;ELISA法检测LPL培养上清中的细胞因子(IFN-γ、IL-4、IL-17A、IL-10)的分泌。结果显示:与WT小鼠相比,IL-21R KO小鼠体重下降更快,生存率明显降低;HE染色显示IL-21R KO小鼠肠管上皮损伤严重、隐窝大面积消失、大量炎性细胞浸润,并侵犯到黏膜肌层;IL-21R KO小鼠肠固有层Th1细胞应答显著增强,相反Th2、Th17和Treg应答明显抑制。因此,IL-21/IL-21R信号通过调节小鼠肠固有层Th细胞应答,在TNBS诱导的肠炎病变形成中发挥重要的保护性作用。 展开更多
关键词 IL-21/IL-21R信号 TNBS诱导的肠炎模型 TH1 TH2 Th17 Treg
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鼠伤寒沙门氏菌群体感应复苏oxyR基因缺失引起的VBNC状态
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作者 廖何斌 徐磊 +4 位作者 夏静若 刘畅 肖丹 马强 郭晓兰 《微生物学杂志》 CAS CSCD 2019年第1期70-76,共7页
探讨鼠伤寒沙门氏菌oxyR基因缺失株引起的VBNC状态及其与群体感应的关系。运用同源重组的方法构建oxyR基因无痕缺失的鼠伤寒沙门氏菌并检测该菌株对H_2O_2的敏感性;将oxyR基因缺失株和亲本株(WT)涂布或滴于LB固体培养基,观察其是否生长... 探讨鼠伤寒沙门氏菌oxyR基因缺失株引起的VBNC状态及其与群体感应的关系。运用同源重组的方法构建oxyR基因无痕缺失的鼠伤寒沙门氏菌并检测该菌株对H_2O_2的敏感性;将oxyR基因缺失株和亲本株(WT)涂布或滴于LB固体培养基,观察其是否生长及其浓度依赖性生长情况;用swimming和swarming平板检测oxyR基因缺失株和WT的运动能力;检测固体培养基和液体培养基中沙门氏菌分解H_2O_2的能力。成功构建了oxyR无痕缺失菌株;oxyR基因缺失株在0.1 mmol/L H_2O_2的LB平板上形成的菌苔发生了变形,在1 mmol/L H_2O_2的LB平板上不能生长,而WT均能生长;6×10~6和6×10~5 cfu/mL的WT涂布于LB平板上能长满菌苔,而等量的oxyR缺失株不能生长菌落;不同浓度的WT滴于LB平板均能形成菌苔,而oxyR缺失株仅在OD_(600)≥10^(-1)浓度时才能形成菌苔;oxyR缺失株泳动距离无显著性变化,而群集运动距离显著性大于WT;固体培养的沙门氏菌比液体培养的沙门氏菌有更强的分解H_2O_2的能力。鼠伤寒沙门氏菌的群体感应系统通过调控其群集运动和H_2O_2分解能力来复苏由oxyR基因缺失引起的VBNC状态。 展开更多
关键词 鼠伤寒沙门氏菌 OXYR VBNC 群体感应 运动性
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Oinical study on thymomas and thymomas with myasthenia gravis 被引量:1
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作者 曾涟乾 《外科研究与新技术》 2003年第2期106-106,共1页
Objective To present the clinical experience in treating thymomas and thymomas with myasthenia gravis (MG). Methods Between 1975 to 1997,285 patients with thymoma were surgically treated. 168 cases of thymus tumor (TT... Objective To present the clinical experience in treating thymomas and thymomas with myasthenia gravis (MG). Methods Between 1975 to 1997,285 patients with thymoma were surgically treated. 168 cases of thymus tumor (TT,Group Ⅰ ) were compared to 90 cases of thymus tumor with myasthenia gravis (TTMG, Group Ⅱ ). Results In group Ⅰ , the average age was 38. 4 years, the youngest was 4 years old. In 93. 4%, the diameter of tumors was 5 cm. 61. 2% were in stages Ⅲ and Ⅳ of clinical pathology. In group Ⅱ ,the average age was 46.2 with the youngest of 20 years old,the diameter of tumor under 3 cm and 5 cm was 34.6 % and 65.6 % . 55.2 % was in stage Ⅰ of clinical pathology. Conclusion Many cases of thymoma in early stage received early treatment. The diagnostic standard of thymoma in early stage is: (1) the diameter of tumor is below 3 cm. (2) In stage I of clinical pathology. The clinical characteristics of TTMG are: short history, the rapid progress, serious MG symptoms,and the high incidence of thymus- 展开更多
关键词 MYASTHENIA GRAVIS THYMUS surgically PATHOLOGY treating INCIDENCE diagnostic
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The VEGF production by dedifferentiated chondrocytes under synovial fluid stimulation from coxarthrosis and femoral neck fracture patients 被引量:1
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作者 于腾波 《外科研究与新技术》 2011年第2期117-117,共1页
Objective To investigate the vascular endothelial growth factor(VEGF)expression level by chondrocytes isolated from patients with osteoarthritis (OA) in hip or femoral neck fracture (FNF) and explore the effect of syn... Objective To investigate the vascular endothelial growth factor(VEGF)expression level by chondrocytes isolated from patients with osteoarthritis (OA) in hip or femoral neck fracture (FNF) and explore the effect of synovial fluid from OA 展开更多
关键词 VEGF The VEGF production by dedifferentiated chondrocytes under synovial fluid stimulation from coxarthrosis and femoral neck fracture patients
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Protective effects of sodium ferulate in vascular endothelial function during cardiopulmonary bypass 被引量:1
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作者 廖健毅 《外科研究与新技术》 2011年第3期167-167,共1页
Objective Cardiopulmonary bypass (CPB) and its related ischemia reperfusion injury may cause endothelial cell injury. To study the protective effects of sodium ferulate in vascular endothelial function during CPB by t... Objective Cardiopulmonary bypass (CPB) and its related ischemia reperfusion injury may cause endothelial cell injury. To study the protective effects of sodium ferulate in vascular endothelial function during CPB by testing the changes of vascular endothelial cell(CEC) ,nitric oxide(NO) and endothelin-1 (ET-1) in children with congenital heart disease. Methods Sixty patients 展开更多
关键词 Protective effects of sodium ferulate in vascular endothelial function during cardiopulmonary bypass CEC
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Clinical features of multiple trauma patients combined with spine and spinal cord injuries 被引量:1
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作者 杨帆 《外科研究与新技术》 2011年第2期106-107,共2页
Objective To analyze the clinical features of the multiple trauma patients combined with spine and spinal cord injuries.Methods A retrospective study was performed in143multiple trauma patients combined with spine and... Objective To analyze the clinical features of the multiple trauma patients combined with spine and spinal cord injuries.Methods A retrospective study was performed in143multiple trauma patients combined with spine and spinal 展开更多
关键词 Clinical features of multiple trauma patients combined with spine and spinal cord injuries ASIA
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Retroperitoneal laparoscopic nephrectomy for kidney tuberculosis 被引量:1
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作者 陈俊星 《外科研究与新技术》 2011年第4期236-236,共1页
Objective To discuss the feasibility and safety of retroperitoneal laparoscopic nephrectomy for treatment of kidney tuberculosis. Methods From March 2005 to February 2009,28 patients with kidney tuberculosis underwent... Objective To discuss the feasibility and safety of retroperitoneal laparoscopic nephrectomy for treatment of kidney tuberculosis. Methods From March 2005 to February 2009,28 patients with kidney tuberculosis underwent retroperitoneal laparoscopic nephrectomy. The patients’ data were reviewed and analyzed. Results 展开更多
关键词 Retroperitoneal laparoscopic nephrectomy for kidney tuberculosis
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Prevention, diagnosis and treatment of biliary complications after liver transplantation 被引量:1
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作者 陈规划 《外科研究与新技术》 2003年第2期129-129,共1页
Objective To study the prevention, diagnosis and treatment of biliary complications after liver transplantation. Methods One hundred and twenty-three recipients who had received liver transplantation between April 199... Objective To study the prevention, diagnosis and treatment of biliary complications after liver transplantation. Methods One hundred and twenty-three recipients who had received liver transplantation between April 1993 and November 2001 were retrospectively reviewed. Results Biliary complication was diagnosed cholangiographyically in 11 patients. Nine patients were cured, 1 patient was unproved and 1 patient died. The incidence for biliary complications was 8.9% (11/123),and the mortality was 0.8% (1/123). The incidence of biliary complicatins due to T tube was 4.2% (5/ 119). The incidence of biliary complications due to hepatic artery was 1. 6% (2/123). In recipients with WIT】3 min and CIT】8 h, the incidence of biliary complications elevated significantly. Conclusion The most important reason for biliary complications was preservative and ischemic injury. While repairing donor liver, damage to the blood supply system of donor liver bile ducts should be avoided. Meticulous T tube placement may reduce 展开更多
关键词 规划
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Liver transplantation in rats using 3 methods of small-for-size grafts
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作者 李江 《外科研究与新技术》 2011年第4期274-274,共1页
Objective To explore a simple and effective way of establishing a 30 % small - for-size liver transplantation in rats. Methods SD rats were selected as donors and recipients. Smallfor - size orthotopic live transplant... Objective To explore a simple and effective way of establishing a 30 % small - for-size liver transplantation in rats. Methods SD rats were selected as donors and recipients. Smallfor - size orthotopic live transplantation was performed using Kamada’s two - cuff method. 展开更多
关键词 ORTHOTOPIC graft establishing RECIPIENT donor HEPATECTOMY STRICTURE shorter BLEEDING AORTA
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Related factors of nosocomial infection for fracture inpatients
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作者 许海平 《外科研究与新技术》 2011年第4期265-265,共1页
Objective To explore related factors between nosocomial infection and fracture inpatients. Methods The clinical data of 1506 fracture inpatients was retrospectively analyzed. Results A total of 51 cases were infected ... Objective To explore related factors between nosocomial infection and fracture inpatients. Methods The clinical data of 1506 fracture inpatients was retrospectively analyzed. Results A total of 51 cases were infected with a rate of 3. 39% . The main infectious area was surgical incision,accounting for 50. 98% ,and urinary 展开更多
关键词 NOSOCOMIAL INCISION accounting URINARY infected INFECTIOUS retrospective emergent curing
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Highly selective peripheral neurotomy as a treatment of cerebral palsy spasm
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作者 韩福友 《外科研究与新技术》 2003年第2期105-105,共1页
Objective In the patients with spastic cerebral palsy, deformities such as club foot, inversion foot and scissor gait often arise form the imbalance in the adductors muscle group of the thigh and leg and their antagon... Objective In the patients with spastic cerebral palsy, deformities such as club foot, inversion foot and scissor gait often arise form the imbalance in the adductors muscle group of the thigh and leg and their antagonistic muscle groups. The incerased muscular tension of the former constitutes the rationale of neurotomy. Methods Highly selective peripheral neurotomy in 38 children who suffered from lower limb deformity secondary to unequal nuscular tension. Twenty-one of the children underwent obturator neurotomy, 27 sciatic neurotomy, and 26 tibial neurotomy. Using electric stimulation, the fasciculi involved were decided and severed. The proportion of fasciculi severed depended on the degree of the muscular tension. Results All patients function improved postoperatiely. 80 % of the patients experienced significant improvement. Conclusion Selective peripheral neurotomy is not only more effective and it also has less complication than selective posterior rhizotomy (SPR) in treating cerebral palsy spasm. 展开更多
关键词 PALSY muscular HIGHLY OBTURATOR suffered children SPASM imbalance experienced decided
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Microsurgical treatment of tuberculum and diaphragma sellae meningiomas by unifrontal interhemispheric approach
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作者 诸葛启钏 《外科研究与新技术》 2003年第2期103-103,共1页
Objective To make an investigation of the effect of microsurgical operation by unifrontal interhemispheric approach for tuberculum and diaphragma sellae meningiomas. Methods 18 patients (11 with tuberculum sellae and ... Objective To make an investigation of the effect of microsurgical operation by unifrontal interhemispheric approach for tuberculum and diaphragma sellae meningiomas. Methods 18 patients (11 with tuberculum sellae and 7 with diaphragma sellae meningiomas) have been treated microsurgically via unifrontal interhemispheric approach Among these cases 4 patients were with tumor maximum diameter less than 3 cm, 13 cases 3 - 6 cm, and only one was more than 6 cm. Results Total resection (Simpson Ⅰ or Ⅱ ) has been performed in 16 patients, the other 2 patients were processed by subtotal resection. There was no operative mortality. After operations, 11 patients gained improvement with visual acuity and visual field, 5 had no obviously improvement, and 2 became worse. 7 patients suffered diabetes insipidus. Conclusion The tuberculum and diaphragma sellae meningiomas have a good prognosis by unifrontal interhemispheric approach which allows a wider operative field and better removal. 6 refs. 展开更多
关键词 suffered OPERATIVE ACUITY MORTALITY SIMPSON PROCESSED prognosis operations worse gained
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Clinical analysis of congenital diaphragmatic hernia in newborn infants:report of 33 cases
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作者 应燕芬 《外科研究与新技术》 2011年第3期165-165,共1页
Objective To review the clinical experience of diagnosis and treatment of the congenital diaphragmatic hernia in newborn infants. Methods Thirty-three neonates were diagnosed having congenital diaphragmatic hernia in ... Objective To review the clinical experience of diagnosis and treatment of the congenital diaphragmatic hernia in newborn infants. Methods Thirty-three neonates were diagnosed having congenital diaphragmatic hernia in our hospitalfrom Jan. 1,2004 to Sept. 30,2009. The clinical data was retrospectively reviewed. Results 21 cases were treated 展开更多
关键词 NEWBORN HERNIA CONGENITAL surgically DYSPLASIA RETRO COOPERATION accept
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One case of renal graft failure associated with cirrhosis following combined kidney and liver transplantation
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作者 杨亦荣 《外科研究与新技术》 2003年第2期136-137,共2页
Objective To explore the feasibility and effect of combined piggyback orthotopic liver and kidney transplantation in patient with renal graft function failure complicated with cirrhosis. Methods One patient with renal... Objective To explore the feasibility and effect of combined piggyback orthotopic liver and kidney transplantation in patient with renal graft function failure complicated with cirrhosis. Methods One patient with renal graft function filure complicated with cirrhosis was resected of renal graft. Daily dose of 50 mg CTX was given 5 days after opeation and continued for 3.5 months. After two couses of plasma exchange, PRA was reduced from 66 % to 23 %. Combined kidney and liver transplantation was performed simultaneously using piggyback orthotopic liver transplantation technique, and the kideny graft was placed retroperitoneally in left iliac fossa. PRA was monitored every 30 min after liver reperfusion. Postoperative irnmunosuppressive therapy consisted of FK506, MMF and steroids. Results The kidney and liver grafts functioned normally after transplantation. PRA was reduced from 23 % to 5 % and maintained at 8%. BHsAg and HCV were returned to negative. Kidney and liver grafts functioned well during a 展开更多
关键词 GRAFT CIRRHOSIS ORTHOTOPIC STEROIDS returned ILIAC monitored KIDNEY resected continued
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