Background:Bongardia chrysogonum(L.)Spach,also known as“Uruf-el-Deek”,has been used by rural communities for the treatment of various diseases and disorders,owing to its wide array of secondary metabolite compositio...Background:Bongardia chrysogonum(L.)Spach,also known as“Uruf-el-Deek”,has been used by rural communities for the treatment of various diseases and disorders,owing to its wide array of secondary metabolite compositions.Methods:The current study was conducted to determine the total polyphenolic content of Bongardia chrysogonum(L.)Spach as well as its potential free radical scavenging and metal-chelating antioxidant capacities.Its leaves,stems,and tubers were separately extracted using ethanol,water,and chloroform;their antioxidant activities were analyzed using in vitro models,including DPPH,ABTS,FRAP,and CUPRAC assays.The total polyphenolic content of the extracts was spectrophotometrically measured.Results:The richest polyphenolic content of the extracts was yielded by tuber chloroform and leaf ethanol extraction(215.09±0.53 mg/g extract as gallic acid equivalent and 240.74±1.12 mg/g extract as quercetin equivalent,respectively).Almost all of the tested extracts exhibited remarkable antioxidant activities,with the highest antioxidant capacity observed in the leaf extracts.On the other hand,the tuber ethanol extract demonstrated the weakest DPPH radical scavenging activity(%inhibition=49.75±0.38,P<0.01),while the tuber chloroform extract showed the weakest ABTS radical scavenging activity(%inhibition=62.61±0.06,P<0.05).These were closely followed by the tuber water extract(%inhibition=63.28±0.65,P<0.01).In terms of their FRAP and CUPRAC assays,the leaf ethanol extract was determined as the best one,with absorbance values of 3.64±0.01 and 2.22±0.01(P<0.01),respectively.Conclusion:Bongardia chrysogonum(L.)Spach possesses rich polyphenolic content and strong antioxidant activities.Thus,it could be a valuable source for the pharmaceutical industry in the prevention and treatment of diseases associated with oxidative stress.展开更多
Since nanoparticle synthesis via chemical and physical methods is expensive and includes hazardous chemicals,biosynthesis has emerged as an environmentally friendly,clean and viable alternative.The present study repor...Since nanoparticle synthesis via chemical and physical methods is expensive and includes hazardous chemicals,biosynthesis has emerged as an environmentally friendly,clean and viable alternative.The present study reports the synthesis of selenium nanoparticles(SeNPs)using Citricoccus sp..For the production of SeNPs,the influence of some parameters(time,pH,temperature and stirring rate)was studied.Optimum synthesis conditions were found as pH 8,24 h reaction time,37℃ and 150 rpm.Synthesized particles were spherical and were 104.46±50.82 nm with a zeta potential of–20.43±0.41 mV.Afterward,the effects of the nanoparticles on oxidative stress biomarkers,hydrogen peroxide(H_(2)O_(2)),malondialdehyde(MDA),chlorophyll(a+b)and growth rate,subsequent to UV-C irradiation on Chlorella vulgaris were investigated.In culture contains nanoparticle and UV-C exposed,the amount of H_(2)O_(2) and MDA decreased on the 1st,3rd and 7th days following UV exposure compared to UV-applied group,while optical density and cell density increased,the amount of chlorophyll(a+b)changes were statistically similar.Consequently,it has been shown that the synthesized SeNPs alleviated the adverse effects of UV stress in microalgae.展开更多
基金supported financially as a project(No.11911/1828MAP1).
文摘Background:Bongardia chrysogonum(L.)Spach,also known as“Uruf-el-Deek”,has been used by rural communities for the treatment of various diseases and disorders,owing to its wide array of secondary metabolite compositions.Methods:The current study was conducted to determine the total polyphenolic content of Bongardia chrysogonum(L.)Spach as well as its potential free radical scavenging and metal-chelating antioxidant capacities.Its leaves,stems,and tubers were separately extracted using ethanol,water,and chloroform;their antioxidant activities were analyzed using in vitro models,including DPPH,ABTS,FRAP,and CUPRAC assays.The total polyphenolic content of the extracts was spectrophotometrically measured.Results:The richest polyphenolic content of the extracts was yielded by tuber chloroform and leaf ethanol extraction(215.09±0.53 mg/g extract as gallic acid equivalent and 240.74±1.12 mg/g extract as quercetin equivalent,respectively).Almost all of the tested extracts exhibited remarkable antioxidant activities,with the highest antioxidant capacity observed in the leaf extracts.On the other hand,the tuber ethanol extract demonstrated the weakest DPPH radical scavenging activity(%inhibition=49.75±0.38,P<0.01),while the tuber chloroform extract showed the weakest ABTS radical scavenging activity(%inhibition=62.61±0.06,P<0.05).These were closely followed by the tuber water extract(%inhibition=63.28±0.65,P<0.01).In terms of their FRAP and CUPRAC assays,the leaf ethanol extract was determined as the best one,with absorbance values of 3.64±0.01 and 2.22±0.01(P<0.01),respectively.Conclusion:Bongardia chrysogonum(L.)Spach possesses rich polyphenolic content and strong antioxidant activities.Thus,it could be a valuable source for the pharmaceutical industry in the prevention and treatment of diseases associated with oxidative stress.
文摘Since nanoparticle synthesis via chemical and physical methods is expensive and includes hazardous chemicals,biosynthesis has emerged as an environmentally friendly,clean and viable alternative.The present study reports the synthesis of selenium nanoparticles(SeNPs)using Citricoccus sp..For the production of SeNPs,the influence of some parameters(time,pH,temperature and stirring rate)was studied.Optimum synthesis conditions were found as pH 8,24 h reaction time,37℃ and 150 rpm.Synthesized particles were spherical and were 104.46±50.82 nm with a zeta potential of–20.43±0.41 mV.Afterward,the effects of the nanoparticles on oxidative stress biomarkers,hydrogen peroxide(H_(2)O_(2)),malondialdehyde(MDA),chlorophyll(a+b)and growth rate,subsequent to UV-C irradiation on Chlorella vulgaris were investigated.In culture contains nanoparticle and UV-C exposed,the amount of H_(2)O_(2) and MDA decreased on the 1st,3rd and 7th days following UV exposure compared to UV-applied group,while optical density and cell density increased,the amount of chlorophyll(a+b)changes were statistically similar.Consequently,it has been shown that the synthesized SeNPs alleviated the adverse effects of UV stress in microalgae.
