The Asia-Pacific region is a home to 60% of the population in the world and to approximately one quarter of people with HIV/AIDS. Close to a million of people has been infected and a half million people died of AIDS a...The Asia-Pacific region is a home to 60% of the population in the world and to approximately one quarter of people with HIV/AIDS. Close to a million of people has been infected and a half million people died of AIDS annually in Asia,becoming the second largest epicenter of global AIDS epidemic. Molecular epidemiology has been useful tool to track a course of HIV spread. In-depth knowledge from the studies on molecular epidemiology elucidates the dynamics of HIV spread and the interrelationship of epidemics in the different regions in Asia.展开更多
Objective: To examine the prognostic factors of progression from HIV to AIDS and AIDS to the death in people living with HIV/AIDS in Iran.Methods: In this registry-based retrospective cohort study, we recruited 28 873...Objective: To examine the prognostic factors of progression from HIV to AIDS and AIDS to the death in people living with HIV/AIDS in Iran.Methods: In this registry-based retrospective cohort study, we recruited 28 873 HIV-infected people from 158 Behavioral Diseases Counseling Centers of Iran.Two outcomes of interest included survival rates from HIV diagnosis to AIDS and from AIDS to the death.We used Kaplan-Meier and Cox regression model to investigate survival rate and factors affecting on survival controlling effect of confounding factors.Results: The one, three, five, and ten-year survival rate from HIV to AIDS were 85%, 73%, 61% and 32%, and for AIDS to death were 90%, 81%, 74% and 55%, respectively.Multivariate Cox regression analysis indicated that the risk of progression from AIDS phase toward death in individuals with CD4 less than 200/mm^3, infected with tuberculosis(TB) and not treated by antiretroviral therapy(ART) was 2.17(95% CI: 1.62-2.90), 1.49(95% CI: 1.01-2.20) and 4.88(95% CI: 3.42-6.96) times higher respectively.The risk of progression to AIDS in patients with baseline CD4 less than 200/mm^3 was 2.32(95% CI: 2.14, 2.51) times higher than patients with CD4 > 200/mm^3(P=0.001).On the other hand, tuberculosis increases the risk of death by 49.0%(P=0.04).The hazard ratio of death in patients who did not receive ART was 4.88(95% CI: 3.42, 6.96) times higher than patients who received ART(P<0.001).Conclusion: The early detection of HIV, the screening and treatment of TB and receiving the ART improve the survival of HIV/AIDS patients significantly, and prevent the transmission of HIV to other people.展开更多
Compared with high infection areas of the world, the total HIV infection rate in China is relatively low. Nonetheless, because of China’s vast territory and large population, the potential infection risk must be take...Compared with high infection areas of the world, the total HIV infection rate in China is relatively low. Nonetheless, because of China’s vast territory and large population, the potential infection risk must be taken seriously. In the next few years, needle sharing among injection drug users will remain the most common route of transmission for the HIV/ AIDS epidemic in China. Unprotected sex is gradually becoming a major route of transmission. China began to imple- ment HAART in 1999 according to international standards. Prior to 2003, there were only about 150 HIV/AIDS patients were treated with HAART in some clinical trials and about 100 HIV/AIDS patients were treated by private sources. Results of those treatments are the scientific basis for development of the therapeutic strategies in China. In March of 2003, the Chinese government initiated China CARES program. In November of 2003, the Chinese Ministry of Health announced a national policy of free ARV treatment to all HIV+ Chinese citizens who were in poverty and required ARV therapy. There are total of 19,456 HIV/AIDS patients received free ARV drugs to date in 159 regions and 441 towns. Current challenges are how to follow-up and evaluate those patients in the clinical settings. The longer the therapy is postponed, the more side effects and the higher probability of drug resistance are going to occur. It remains unclear, therefore, when HAART regimen should be started in the HIV/AIDS population in China.展开更多
Several reviews have focused on the nature of HIV infection and its spread in various geographical regions of China. In contrast, this review provides a comprehensive update on the prevalence of multiple HIV- 1 subtyp...Several reviews have focused on the nature of HIV infection and its spread in various geographical regions of China. In contrast, this review provides a comprehensive update on the prevalence of multiple HIV- 1 subtypes, consequent emergence of recombinant and novel forms of HIV- 1 in China, and the implications this may have on HIV diversity and the development of effective vaccines. In addition it also examines the dissemination of primary drug resistance in therapy naive patients, as well as co-infections with two other important viruses-hepatitis B and C. The main purpose of this review is to provide a current snapshot of HIV-1 pathogenesis in China and possibly shed some light on the future of HIV evolution, and potential challenges for future vaccine and anti-retroviral therapeutics against HIV strains in this area.展开更多
OBJECTIVE: To evaluate the effect of Fuzhengpaidu granule (FZPDG) on immune activation molecules CD38 and human leukocyte antigen-D related (HLA-DR) on CD4+ and CD8+ cells in HIV/AIDS patients, and to explore the unde...OBJECTIVE: To evaluate the effect of Fuzhengpaidu granule (FZPDG) on immune activation molecules CD38 and human leukocyte antigen-D related (HLA-DR) on CD4+ and CD8+ cells in HIV/AIDS patients, and to explore the underlying mechanism of this therapy. METHODS: Plasma changes in CD3+, CD4+, CD8+, CD3+CD4+CD38+, CD3+CD4+HLA-DR+, CD3+ CD8+CD38+, and CD3+CD8+HLA-DR+levels in HIV/ AIDS patients treated with FZPDG for six months were examined by flow cytometry and compared with levels in healthy controls. RESULTS: The clinical trial included 34 outpatients with HIV/AIDS. Before treatment, plasma levels of CD38+ and HLA-DR+ on CD4/CD8 cells were higherthan those in 28 health controls (P<0.05). There were no significant changes in serum levels of CD3+, CD4+, and CD8+ T cells between pretreatment baseline versus after treatment, which were 82.85% ± 5.41% , 14.57% ± 10.31% and 54.55% ± 11.43% before treatment and 79.15% ± 8.21% , 19.96% ± 9.58% and 56.36% ± 11.67% after treatment, respectively (P>0.05). Plasma levels of CD3+ CD4+CD38+and CD3+CD4+HLA-DR+were 2.3%± 2.2% and 7.8%±5.5% before treatment and 1.2%± 0.8% and 2.6%±1.0% after treatment, respectively. Plasma levels of CD3+CD8+CD38+ and CD3+CD8+ HLA-DR+ were 41.4%±13.4% and 17.8%±11.3% beforetreatment,whichchangedto27.1%±10.2%and 3.8%±2.4%aftertreatment,respectively(P<0.05). CONCLUSION: HIV/AIDS patients exhibited an immune activation profile following FZPDG treatment. A potential mechanism of action for FZPDG appears to lie in its ability to up-regulate CD38 and HLA-DR levels on CD4+ T cells, and down-regulate them on CD8+ cells, thereby modulating immune activation of CD4+and CD8+T cells.展开更多
The robustness of cargo ship transportation networks is essential to the stability of the world trade system. The current research mainly focuses on the coarse-grained, holistic cargo ship transportation network while...The robustness of cargo ship transportation networks is essential to the stability of the world trade system. The current research mainly focuses on the coarse-grained, holistic cargo ship transportation network while ignoring the structural diversity of different sub-networks. In this paper, we evaluate the robustness of the global cargo ship transportation network based on the most recent Automatic Identification System（AIS） data available. First, we subdivide three typical cargo ship transportation networks（i.e., oil tanker, container ship and bulk carrier） from the original cargo ship transportation network. Then, we design statistical indices based on complex network theory and employ four attack strategies, including random attack and three intentional attacks（i.e., degree-based attack, betweenness-based attack and flux-based attack） to evaluate the robustness of the three typical cargo ship transportation networks. Finally, we compare the integrity of the remaining ports of the network when a small proportion of ports lose their function. The results show that 1） compared with the holistic cargo ship transportation network, the fine-grain-based cargo ship transportation networks can fully reflect the pattern and process of global cargo transportation; 2） different cargo ship networks behave heterogeneously in terms of their robustness, with the container network being the weakest and the bulk carrier network being the strongest; and 3） small-scale intentional attacks may have significant influence on the integrity of the container network but a minor impact on the bulk carrier and oil tanker transportation networks. These conclusions can help improve the decision support capabilities in maritime transportation planning and emergency response and facilitate the establishment of a more reliable maritime transportation system.Abstract： The robustness of cargo ship transportation networks is essential to the stability of the world trade system. The current research mainly focuses on the coarse-grained, holistic cargo ship transportation network while ignoring the structural diversity of different sub-networks. In this paper, we evaluate the robustness of the global cargo ship transporta- tion network based on the most recent Automatic Identification System （AIS） data available. First, we subdivide three typical cargo ship transportation networks （i.e., oil tanker, container ship and bulk carrier） from the original cargo ship transportation network. Then, we design statistical indices based on complex network theory and employ four attack strategies, in- cluding random attack and three intentional attacks （i.e., degree-based attack, between- ness-based attack and flux-based attack） to evaluate the robustness of the three typical cargo ship transportation networks. Finally, we compare the integrity of the remaining ports of the network when a small proportion of ports lose their function. The results show that 1） com- pared with the holistic cargo ship transportation network, the fine-grain-based cargo ship transportation networks can fully reflect the pattern and process of global cargo transportation 2） different cargo ship networks behave heterogeneously in terms of their robustness, with the container network being the weakest and the bulk carrier network being the strongest; and 3） small-scale intentional attacks may have significant influence on the integrity of the con- tainer network but a minor impact on the bulk carrier and oil tanker transportation networks.These conclusions can help improve the decision support capabilities in maritime transportation planning and emergency response and facilitate the establishment of a more reliable maritime transportation system.展开更多
Objective To investigate the reproductive and sexual health situation, including knowledge, attitudes, and behaviors, among a population-based sample of internal migrant workers in China. Methods A cross-sectional sur...Objective To investigate the reproductive and sexual health situation, including knowledge, attitudes, and behaviors, among a population-based sample of internal migrant workers in China. Methods A cross-sectional survey of 4 900 rural-to-urban migrants in 6 provinces of China was conducted. Participants completed a 30-min semi-structured questionnaire about contraceptive practices, sexual behavior, and HIV-related knowledge. Results Migrants lacked knowledge of reproductive and sexual health issues. Among those who had heard any sexually transmitted infections （STIs）, only 79.1%, 46.2%, 86.1%, 14.5% and 82.2%, respectively, knew that gonorrhea, condyloma, syphilis, chancroid, and AIDS were STIs. About three-quarters of participants had not used any contraceptive method at sexual debut. Among current users of contraceptive methods, 85.5% indicated that they were satisfied with the method. Before adoption of a contraceptive method, 46.6% of the migrant workers were unaware of the advantages/ disadvantages of the method and 75.3% had no knowledge of emergency contraception. Nearly one-quarter （23.4%） reported that they had premarital sex. Among migrants who were sexually active one month prior to the survey, only 14.0% reported that they had used condoms. Conclusion The limited sexual and reproductive health knowledge and unmet reproductive health services of migrant workers in China underscore the need for a comprehensive package of sexual and reproductive health interventions that combine cognitive and behavioral skills training and target both migrants and health care providers.展开更多
AIM: To determine the distribution of Hepatitis B virus (HBV) genotypes in Benin, and to clarify the virological characteristics of the dominant genotype.METHODS: Among 500 blood donors in Benin, 21 HBsAg-positive don...AIM: To determine the distribution of Hepatitis B virus (HBV) genotypes in Benin, and to clarify the virological characteristics of the dominant genotype.METHODS: Among 500 blood donors in Benin, 21 HBsAg-positive donors were enrolled in the study. HBV genotypes were determined by enzyme immunoassay and restriction fragment length polymorphism. Complete genome sequences were determined by PCR and direct sequencing.RESULTS: HBV genotype E (HBV/E) was detected in 20/21 (95.2%), and HBV/A in 1/21 (4.8%). From the age-specific prevalence of HBeAg to anti-HBe seroconversion (SC) in 19 HBV/E subjects, SC was estimated to occur frequently in late teens in HBV/E.The comparison of four complete HBV/E genomes from HBeAg-positive subjects in this study and five HBV/E sequences recruited from the database revealed that HBV/E was distributed throughout West Africa with very low genetic divers ity (nucleotide homology 96.7-99.2%).Based on the sequences in the basic core promoter (BCP)to precore region of the nine HBV/E isolates compared to those of the other genotypes, a nucleotide substitution in the BCP, G1757A, was observed in HBV/E.CONCLUSION: HBV/E is predominant in the Republic of Benin, and SC is estimated to occur in late teens in HBV/E. The specific nucleotide substitution G1757A in BCP, which might influence the virological characteristics,is observed in HBV/E.展开更多
HIV fusion inhibitors are promising therapeutic agents for AIDS treatment. One fusion inhibitor has been approved as anti-HIV drug, while more of them are in preclinical studies or clinical trials. Highly active fusio...HIV fusion inhibitors are promising therapeutic agents for AIDS treatment. One fusion inhibitor has been approved as anti-HIV drug, while more of them are in preclinical studies or clinical trials. Highly active fusion inhibitors with excellent pharmacokinetic properties are still needed for development of anti-HIV drugs. We found that all-hydrocarbon staples inserted in SC34 EK could not only enhance the inhibitory activity of inhibitors against HIV-1, but also improve protease resistance. Further study revealed that SC34 EK-1 containing a staple was a potent fusion inhibitor with IC;value of 0.04-6.4 nmol/L towards diverse HIV-1 subtypes and half-life value of 112 min against protease hydrolysis. X-ray crystallography studies indicated that introduction of a hydrocarbon staple in SC34 EK could make the amino acid at the interaction surface form perfect conformation to promote inhibitor peptide interacting with target.展开更多
Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around NAb epitopes and deletions of variable regions in env. The immunogenicities of the generated mutants were evaluated usi...Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around NAb epitopes and deletions of variable regions in env. The immunogenicities of the generated mutants were evaluated using single-cycle infection neutralization assays with two pseudoviruses and IFN-γ ELISPOT. Overall, five mutants (dWt, M2, M5-2, M5-1 and dM7) induced higher neutralization activities for both pseudoviruses than plasmid Wt, while only two of the mutants (dWt and M5-2) showed significant differences (P<0.05). Two mutants (M2 and dM2) induced more Env-specific T cells than plasmid Wt. Statistically however, significance was only reached for mutant M2. Thus, properly modified HIV-1 Env may have the potential to induce potent cellular and humoral immune responses.展开更多
Gastric organoids are models created in the laboratory using stem cells and sophisticated three-dimensional cell culture techniques.These models have shown great promise in providing valuable insights into gastric phy...Gastric organoids are models created in the laboratory using stem cells and sophisticated three-dimensional cell culture techniques.These models have shown great promise in providing valuable insights into gastric physiology and advanced disease research.This review comprehensively summarizes and analyzes the research advances in culture methods and techniques for adult stem cells and induced pluripotent stem cell-derived organoids,and patient-derived organoids.The potential value of gastric organoids in studying the pathogenesis of stomach-related diseases and facilitating drug screening is initially discussed.The construction of gastric organoids involves several key steps,including cell extraction and culture,three-dimensional structure formation,and functional expression.Simulating the structure and function of the human stomach by disease modeling with gastric organoids provides a platform to study the mechanism of gastric cancer induction by Helicobacter pylori.In addition,in drug screening and development,gastric organoids can be used as a key tool to evaluate drug efficacy and toxicity in preclinical trials.They can also be used for precision medicine according to the specific conditions of patients with gastric cancer,to assess drug resistance,and to predict the possibility of adverse reactions.However,despite the impressive progress in the field of gastric organoids,there are still many unknowns that need to be addressed,especially in the field of regenerative medicine.Meanwhile,the reproducibility and consistency of organoid cultures are major challenges that must be overcome.These challenges have had a significant impact on the development of gastric organoids.Nonetheless,as technology continues to advance,we can foresee more comprehensive research in the construction of gastric organoids.Such research will provide better solutions for the treatment of stomach-related diseases and personalized medicine.展开更多
Direct-acting antiviral(DAA)therapies are efficacious for the achievement of sustained virologic response(SVR)in almost all treated hepatitis C virus(HCV)-infected patients.However,the impacts of HCV eradication on im...Direct-acting antiviral(DAA)therapies are efficacious for the achievement of sustained virologic response(SVR)in almost all treated hepatitis C virus(HCV)-infected patients.However,the impacts of HCV eradication on immune function and chronic immune activation in the long-term remain controversial and limited,especially in patients co-infected with human immunodeficiency virus(HIV).Indeed,although restoration of many immune responses clearly can be observed,several features of immune perturbations persist over time after HCV clearance.Understanding the degree and reasons of the partial recovery of the immune system in chronic HCV/HIV coinfection after HCV elimination is pivotal to avoid disease progression and possible long-term clinical outcomes in cured patients,as well as contributing to the development of immunotherapy drug design.展开更多
Prepore formation is hypothesized to be an obligate step in the insertion of Cry1Ab toxin into insect brush border membrane vesicles. We examined the architecture of the putative prepore when isolated using the publis...Prepore formation is hypothesized to be an obligate step in the insertion of Cry1Ab toxin into insect brush border membrane vesicles. We examined the architecture of the putative prepore when isolated using the published protocols [1] [2]. Our results demonstrate that the putative prepore form of Cry1Ab is a combination of receptor proteins attached to the toxin, when purified. The results also suggest that this prepore form as prepared by the methods published is different from other membrane-extracted oligomeric forms of Cry toxins and prepore of other toxins in general. While most other known prepores are composed of multimers of a single protein, the Cry1Ab prepore, as generated, is a protein-receptor complex oligomer and monomers of Cry toxins.展开更多
An effcient formal synthesis of（＋）-tashiromine was achieved by employing an intermolecular asymmetric Mannich-type reaction as the key step.Concurrently,a novel approach toward the total synthesis of（＋）-stemoami...An effcient formal synthesis of（＋）-tashiromine was achieved by employing an intermolecular asymmetric Mannich-type reaction as the key step.Concurrently,a novel approach toward the total synthesis of（＋）-stemoamide through dyotropic rearrangement of 3,4-cis-b-lactone was also explored.展开更多
OBJECTIVE: To evaluate the psychometric properties of the Persian version of the medical outcome study human immunodeficiency virus(MOS-HIV)health survey among people living with HIV or acquired immune deficiency synd...OBJECTIVE: To evaluate the psychometric properties of the Persian version of the medical outcome study human immunodeficiency virus(MOS-HIV)health survey among people living with HIV or acquired immune deficiency syndrome(HIV/AIDS) in Iran is our main goal in this study.METHODS: The MOS-HIV questionnaire was translated into Persian using backward translation and the Persian version was filled by 100 HIV infected patients. Reliability of the questionnaire was estimated using Cronbach's alpha coefficient for the total questionnaire and for each dimension. Convergent, discriminant and construct validity were performed. Comparisons were performed between the subscales of the questionnaire in Iran and some other countries with t-test.RESULTS: The overall Cronbach's alpha in this study was 0.90 and the range of this coefficient was from 0.71 to 0.86 across the subscales with two exceptions, general health perception and role functioning, which were at 0.65 and 0.61. Convergent and discriminant validity were excellent in all domains. The two factor confirmatory factor analysis showed a good fit based on standardized estimates of factor loading, incremental and absolute fit indices.CONCLUSION: The Persian version of the MOS-HIV had good reliability and validity in measuring health related quality of life in people living with HIV in Iran. Furthermore, quality of life for HIV infected patients was very low in comparing with the people in some other countries.展开更多
基金Grant support from Ministry of Health, Labour and Welfare and Ministry of Education, Science and Technology in JapanJapanese Foundation for AIDS Prevention.
文摘The Asia-Pacific region is a home to 60% of the population in the world and to approximately one quarter of people with HIV/AIDS. Close to a million of people has been infected and a half million people died of AIDS annually in Asia,becoming the second largest epicenter of global AIDS epidemic. Molecular epidemiology has been useful tool to track a course of HIV spread. In-depth knowledge from the studies on molecular epidemiology elucidates the dynamics of HIV spread and the interrelationship of epidemics in the different regions in Asia.
基金The study funded by the Vice-chancellor for Research and Technology,Hamadan University of Medical Sciences(No.9508044500)
文摘Objective: To examine the prognostic factors of progression from HIV to AIDS and AIDS to the death in people living with HIV/AIDS in Iran.Methods: In this registry-based retrospective cohort study, we recruited 28 873 HIV-infected people from 158 Behavioral Diseases Counseling Centers of Iran.Two outcomes of interest included survival rates from HIV diagnosis to AIDS and from AIDS to the death.We used Kaplan-Meier and Cox regression model to investigate survival rate and factors affecting on survival controlling effect of confounding factors.Results: The one, three, five, and ten-year survival rate from HIV to AIDS were 85%, 73%, 61% and 32%, and for AIDS to death were 90%, 81%, 74% and 55%, respectively.Multivariate Cox regression analysis indicated that the risk of progression from AIDS phase toward death in individuals with CD4 less than 200/mm^3, infected with tuberculosis(TB) and not treated by antiretroviral therapy(ART) was 2.17(95% CI: 1.62-2.90), 1.49(95% CI: 1.01-2.20) and 4.88(95% CI: 3.42-6.96) times higher respectively.The risk of progression to AIDS in patients with baseline CD4 less than 200/mm^3 was 2.32(95% CI: 2.14, 2.51) times higher than patients with CD4 > 200/mm^3(P=0.001).On the other hand, tuberculosis increases the risk of death by 49.0%(P=0.04).The hazard ratio of death in patients who did not receive ART was 4.88(95% CI: 3.42, 6.96) times higher than patients who received ART(P<0.001).Conclusion: The early detection of HIV, the screening and treatment of TB and receiving the ART improve the survival of HIV/AIDS patients significantly, and prevent the transmission of HIV to other people.
文摘Compared with high infection areas of the world, the total HIV infection rate in China is relatively low. Nonetheless, because of China’s vast territory and large population, the potential infection risk must be taken seriously. In the next few years, needle sharing among injection drug users will remain the most common route of transmission for the HIV/ AIDS epidemic in China. Unprotected sex is gradually becoming a major route of transmission. China began to imple- ment HAART in 1999 according to international standards. Prior to 2003, there were only about 150 HIV/AIDS patients were treated with HAART in some clinical trials and about 100 HIV/AIDS patients were treated by private sources. Results of those treatments are the scientific basis for development of the therapeutic strategies in China. In March of 2003, the Chinese government initiated China CARES program. In November of 2003, the Chinese Ministry of Health announced a national policy of free ARV treatment to all HIV+ Chinese citizens who were in poverty and required ARV therapy. There are total of 19,456 HIV/AIDS patients received free ARV drugs to date in 159 regions and 441 towns. Current challenges are how to follow-up and evaluate those patients in the clinical settings. The longer the therapy is postponed, the more side effects and the higher probability of drug resistance are going to occur. It remains unclear, therefore, when HAART regimen should be started in the HIV/AIDS population in China.
文摘Several reviews have focused on the nature of HIV infection and its spread in various geographical regions of China. In contrast, this review provides a comprehensive update on the prevalence of multiple HIV- 1 subtypes, consequent emergence of recombinant and novel forms of HIV- 1 in China, and the implications this may have on HIV diversity and the development of effective vaccines. In addition it also examines the dissemination of primary drug resistance in therapy naive patients, as well as co-infections with two other important viruses-hepatitis B and C. The main purpose of this review is to provide a current snapshot of HIV-1 pathogenesis in China and possibly shed some light on the future of HIV evolution, and potential challenges for future vaccine and anti-retroviral therapeutics against HIV strains in this area.
基金Supported by the Natural Science Foundation of China(No.30901906)the China Postdoctoral Science Foundation(No. 20080440743)
文摘OBJECTIVE: To evaluate the effect of Fuzhengpaidu granule (FZPDG) on immune activation molecules CD38 and human leukocyte antigen-D related (HLA-DR) on CD4+ and CD8+ cells in HIV/AIDS patients, and to explore the underlying mechanism of this therapy. METHODS: Plasma changes in CD3+, CD4+, CD8+, CD3+CD4+CD38+, CD3+CD4+HLA-DR+, CD3+ CD8+CD38+, and CD3+CD8+HLA-DR+levels in HIV/ AIDS patients treated with FZPDG for six months were examined by flow cytometry and compared with levels in healthy controls. RESULTS: The clinical trial included 34 outpatients with HIV/AIDS. Before treatment, plasma levels of CD38+ and HLA-DR+ on CD4/CD8 cells were higherthan those in 28 health controls (P<0.05). There were no significant changes in serum levels of CD3+, CD4+, and CD8+ T cells between pretreatment baseline versus after treatment, which were 82.85% ± 5.41% , 14.57% ± 10.31% and 54.55% ± 11.43% before treatment and 79.15% ± 8.21% , 19.96% ± 9.58% and 56.36% ± 11.67% after treatment, respectively (P>0.05). Plasma levels of CD3+ CD4+CD38+and CD3+CD4+HLA-DR+were 2.3%± 2.2% and 7.8%±5.5% before treatment and 1.2%± 0.8% and 2.6%±1.0% after treatment, respectively. Plasma levels of CD3+CD8+CD38+ and CD3+CD8+ HLA-DR+ were 41.4%±13.4% and 17.8%±11.3% beforetreatment,whichchangedto27.1%±10.2%and 3.8%±2.4%aftertreatment,respectively(P<0.05). CONCLUSION: HIV/AIDS patients exhibited an immune activation profile following FZPDG treatment. A potential mechanism of action for FZPDG appears to lie in its ability to up-regulate CD38 and HLA-DR levels on CD4+ T cells, and down-regulate them on CD8+ cells, thereby modulating immune activation of CD4+and CD8+T cells.
基金Key Project of the Chinese Academy of Sciences,No.ZDRW-ZS-2016-6-3National Natural Science Foundation of China,No.41501490
文摘The robustness of cargo ship transportation networks is essential to the stability of the world trade system. The current research mainly focuses on the coarse-grained, holistic cargo ship transportation network while ignoring the structural diversity of different sub-networks. In this paper, we evaluate the robustness of the global cargo ship transportation network based on the most recent Automatic Identification System（AIS） data available. First, we subdivide three typical cargo ship transportation networks（i.e., oil tanker, container ship and bulk carrier） from the original cargo ship transportation network. Then, we design statistical indices based on complex network theory and employ four attack strategies, including random attack and three intentional attacks（i.e., degree-based attack, betweenness-based attack and flux-based attack） to evaluate the robustness of the three typical cargo ship transportation networks. Finally, we compare the integrity of the remaining ports of the network when a small proportion of ports lose their function. The results show that 1） compared with the holistic cargo ship transportation network, the fine-grain-based cargo ship transportation networks can fully reflect the pattern and process of global cargo transportation; 2） different cargo ship networks behave heterogeneously in terms of their robustness, with the container network being the weakest and the bulk carrier network being the strongest; and 3） small-scale intentional attacks may have significant influence on the integrity of the container network but a minor impact on the bulk carrier and oil tanker transportation networks. These conclusions can help improve the decision support capabilities in maritime transportation planning and emergency response and facilitate the establishment of a more reliable maritime transportation system.Abstract： The robustness of cargo ship transportation networks is essential to the stability of the world trade system. The current research mainly focuses on the coarse-grained, holistic cargo ship transportation network while ignoring the structural diversity of different sub-networks. In this paper, we evaluate the robustness of the global cargo ship transporta- tion network based on the most recent Automatic Identification System （AIS） data available. First, we subdivide three typical cargo ship transportation networks （i.e., oil tanker, container ship and bulk carrier） from the original cargo ship transportation network. Then, we design statistical indices based on complex network theory and employ four attack strategies, in- cluding random attack and three intentional attacks （i.e., degree-based attack, between- ness-based attack and flux-based attack） to evaluate the robustness of the three typical cargo ship transportation networks. Finally, we compare the integrity of the remaining ports of the network when a small proportion of ports lose their function. The results show that 1） com- pared with the holistic cargo ship transportation network, the fine-grain-based cargo ship transportation networks can fully reflect the pattern and process of global cargo transportation 2） different cargo ship networks behave heterogeneously in terms of their robustness, with the container network being the weakest and the bulk carrier network being the strongest; and 3） small-scale intentional attacks may have significant influence on the integrity of the con- tainer network but a minor impact on the bulk carrier and oil tanker transportation networks.These conclusions can help improve the decision support capabilities in maritime transportation planning and emergency response and facilitate the establishment of a more reliable maritime transportation system.
文摘Objective To investigate the reproductive and sexual health situation, including knowledge, attitudes, and behaviors, among a population-based sample of internal migrant workers in China. Methods A cross-sectional survey of 4 900 rural-to-urban migrants in 6 provinces of China was conducted. Participants completed a 30-min semi-structured questionnaire about contraceptive practices, sexual behavior, and HIV-related knowledge. Results Migrants lacked knowledge of reproductive and sexual health issues. Among those who had heard any sexually transmitted infections （STIs）, only 79.1%, 46.2%, 86.1%, 14.5% and 82.2%, respectively, knew that gonorrhea, condyloma, syphilis, chancroid, and AIDS were STIs. About three-quarters of participants had not used any contraceptive method at sexual debut. Among current users of contraceptive methods, 85.5% indicated that they were satisfied with the method. Before adoption of a contraceptive method, 46.6% of the migrant workers were unaware of the advantages/ disadvantages of the method and 75.3% had no knowledge of emergency contraception. Nearly one-quarter （23.4%） reported that they had premarital sex. Among migrants who were sexually active one month prior to the survey, only 14.0% reported that they had used condoms. Conclusion The limited sexual and reproductive health knowledge and unmet reproductive health services of migrant workers in China underscore the need for a comprehensive package of sexual and reproductive health interventions that combine cognitive and behavioral skills training and target both migrants and health care providers.
文摘AIM: To determine the distribution of Hepatitis B virus (HBV) genotypes in Benin, and to clarify the virological characteristics of the dominant genotype.METHODS: Among 500 blood donors in Benin, 21 HBsAg-positive donors were enrolled in the study. HBV genotypes were determined by enzyme immunoassay and restriction fragment length polymorphism. Complete genome sequences were determined by PCR and direct sequencing.RESULTS: HBV genotype E (HBV/E) was detected in 20/21 (95.2%), and HBV/A in 1/21 (4.8%). From the age-specific prevalence of HBeAg to anti-HBe seroconversion (SC) in 19 HBV/E subjects, SC was estimated to occur frequently in late teens in HBV/E.The comparison of four complete HBV/E genomes from HBeAg-positive subjects in this study and five HBV/E sequences recruited from the database revealed that HBV/E was distributed throughout West Africa with very low genetic divers ity (nucleotide homology 96.7-99.2%).Based on the sequences in the basic core promoter (BCP)to precore region of the nine HBV/E isolates compared to those of the other genotypes, a nucleotide substitution in the BCP, G1757A, was observed in HBV/E.CONCLUSION: HBV/E is predominant in the Republic of Benin, and SC is estimated to occur in late teens in HBV/E. The specific nucleotide substitution G1757A in BCP, which might influence the virological characteristics,is observed in HBV/E.
基金supported by the National Natural Science Foundation of China (No. 21602121)the Natural Science Foundation of Inner Mongolia (No. 2016BS0201)+2 种基金the Inner Mongolia Autonomous Region Higher School Youth scientific Talents Support Project(No. NJYT-17-B22)the Research Funds of Baotou Medical College(Nos. BSJJ201620, BYJJ-YF 201707)Beijing Tongzhou District Science and Technology Project(No. KJ2017CX039-14)
文摘HIV fusion inhibitors are promising therapeutic agents for AIDS treatment. One fusion inhibitor has been approved as anti-HIV drug, while more of them are in preclinical studies or clinical trials. Highly active fusion inhibitors with excellent pharmacokinetic properties are still needed for development of anti-HIV drugs. We found that all-hydrocarbon staples inserted in SC34 EK could not only enhance the inhibitory activity of inhibitors against HIV-1, but also improve protease resistance. Further study revealed that SC34 EK-1 containing a staple was a potent fusion inhibitor with IC;value of 0.04-6.4 nmol/L towards diverse HIV-1 subtypes and half-life value of 112 min against protease hydrolysis. X-ray crystallography studies indicated that introduction of a hydrocarbon staple in SC34 EK could make the amino acid at the interaction surface form perfect conformation to promote inhibitor peptide interacting with target.
文摘Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around NAb epitopes and deletions of variable regions in env. The immunogenicities of the generated mutants were evaluated using single-cycle infection neutralization assays with two pseudoviruses and IFN-γ ELISPOT. Overall, five mutants (dWt, M2, M5-2, M5-1 and dM7) induced higher neutralization activities for both pseudoviruses than plasmid Wt, while only two of the mutants (dWt and M5-2) showed significant differences (P<0.05). Two mutants (M2 and dM2) induced more Env-specific T cells than plasmid Wt. Statistically however, significance was only reached for mutant M2. Thus, properly modified HIV-1 Env may have the potential to induce potent cellular and humoral immune responses.
基金Supported by Chinese Medicine Service System and Capacity Building(Key Project with Chinese Medicine Characteristics and Advantages,Ruikang Hospital,2023)Guangxi Science and Technology Major Project during the 14th five-year Plan,No.Guike AA22096028.
文摘Gastric organoids are models created in the laboratory using stem cells and sophisticated three-dimensional cell culture techniques.These models have shown great promise in providing valuable insights into gastric physiology and advanced disease research.This review comprehensively summarizes and analyzes the research advances in culture methods and techniques for adult stem cells and induced pluripotent stem cell-derived organoids,and patient-derived organoids.The potential value of gastric organoids in studying the pathogenesis of stomach-related diseases and facilitating drug screening is initially discussed.The construction of gastric organoids involves several key steps,including cell extraction and culture,three-dimensional structure formation,and functional expression.Simulating the structure and function of the human stomach by disease modeling with gastric organoids provides a platform to study the mechanism of gastric cancer induction by Helicobacter pylori.In addition,in drug screening and development,gastric organoids can be used as a key tool to evaluate drug efficacy and toxicity in preclinical trials.They can also be used for precision medicine according to the specific conditions of patients with gastric cancer,to assess drug resistance,and to predict the possibility of adverse reactions.However,despite the impressive progress in the field of gastric organoids,there are still many unknowns that need to be addressed,especially in the field of regenerative medicine.Meanwhile,the reproducibility and consistency of organoid cultures are major challenges that must be overcome.These challenges have had a significant impact on the development of gastric organoids.Nonetheless,as technology continues to advance,we can foresee more comprehensive research in the construction of gastric organoids.Such research will provide better solutions for the treatment of stomach-related diseases and personalized medicine.
文摘Direct-acting antiviral(DAA)therapies are efficacious for the achievement of sustained virologic response(SVR)in almost all treated hepatitis C virus(HCV)-infected patients.However,the impacts of HCV eradication on immune function and chronic immune activation in the long-term remain controversial and limited,especially in patients co-infected with human immunodeficiency virus(HIV).Indeed,although restoration of many immune responses clearly can be observed,several features of immune perturbations persist over time after HCV clearance.Understanding the degree and reasons of the partial recovery of the immune system in chronic HCV/HIV coinfection after HCV elimination is pivotal to avoid disease progression and possible long-term clinical outcomes in cured patients,as well as contributing to the development of immunotherapy drug design.
文摘Prepore formation is hypothesized to be an obligate step in the insertion of Cry1Ab toxin into insect brush border membrane vesicles. We examined the architecture of the putative prepore when isolated using the published protocols [1] [2]. Our results demonstrate that the putative prepore form of Cry1Ab is a combination of receptor proteins attached to the toxin, when purified. The results also suggest that this prepore form as prepared by the methods published is different from other membrane-extracted oligomeric forms of Cry toxins and prepore of other toxins in general. While most other known prepores are composed of multimers of a single protein, the Cry1Ab prepore, as generated, is a protein-receptor complex oligomer and monomers of Cry toxins.
基金the financial supports from Beijing Natural Science Foundation (No. 2132037)NSFC (Nos. 21102081, 21272133)+1 种基金New Teachers’ Fund for Doctor Stations Ministry of Education (No. 20110002120011)Scientifc Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education (No. 20121027968)
文摘An effcient formal synthesis of（＋）-tashiromine was achieved by employing an intermolecular asymmetric Mannich-type reaction as the key step.Concurrently,a novel approach toward the total synthesis of（＋）-stemoamide through dyotropic rearrangement of 3,4-cis-b-lactone was also explored.
基金Supported by the grant from Shiraz University of Medical Sciences Research Council(No.88-4665)
文摘OBJECTIVE: To evaluate the psychometric properties of the Persian version of the medical outcome study human immunodeficiency virus(MOS-HIV)health survey among people living with HIV or acquired immune deficiency syndrome(HIV/AIDS) in Iran is our main goal in this study.METHODS: The MOS-HIV questionnaire was translated into Persian using backward translation and the Persian version was filled by 100 HIV infected patients. Reliability of the questionnaire was estimated using Cronbach's alpha coefficient for the total questionnaire and for each dimension. Convergent, discriminant and construct validity were performed. Comparisons were performed between the subscales of the questionnaire in Iran and some other countries with t-test.RESULTS: The overall Cronbach's alpha in this study was 0.90 and the range of this coefficient was from 0.71 to 0.86 across the subscales with two exceptions, general health perception and role functioning, which were at 0.65 and 0.61. Convergent and discriminant validity were excellent in all domains. The two factor confirmatory factor analysis showed a good fit based on standardized estimates of factor loading, incremental and absolute fit indices.CONCLUSION: The Persian version of the MOS-HIV had good reliability and validity in measuring health related quality of life in people living with HIV in Iran. Furthermore, quality of life for HIV infected patients was very low in comparing with the people in some other countries.
