Graphical abstracts(GAs)are emerging as a pivotal tool in medical literature,enhancing the dissemination and comprehension of complex clinical data through visual summaries.This editorial highlights the significant ad...Graphical abstracts(GAs)are emerging as a pivotal tool in medical literature,enhancing the dissemination and comprehension of complex clinical data through visual summaries.This editorial highlights the significant advantages of GAs,including improved clarity,increased reader engagement,and enhanced visibility of research findings.By transforming intricate scientific data into accessible visual formats,these abstracts facilitate quick and effective knowledge transfer,crucial in clinical decision-making and patient care.However,challenges such as potential data misrepresentation due to oversimplification,the skill gap in graphic design among researchers,and the lack of standardized creation guidelines pose barriers to their widespread adoption.Additionally,while software such as Adobe Illustrator,BioRender,and Canva are commonly employed to create these visuals,not all researchers may be proficient in their use.To address these issues,we recommend that academic journals establish clear guidelines and provide necessary design training to researchers.This proactive approach will ensure the creation of high-quality GAs,promote their standardization,and expand their use in clinical reporting,ultimately benefiting the medical community and improving healthcare outcomes.展开更多
Two thioamino acids and four fiuorinated amino acids were employed to substitute either partially or entirely the Ile_(2),Ser_(3),Ile_(6),and Ser_(7) residues of Leu_(10)-teixobactin to prepare ten analogues and the b...Two thioamino acids and four fiuorinated amino acids were employed to substitute either partially or entirely the Ile_(2),Ser_(3),Ile_(6),and Ser_(7) residues of Leu_(10)-teixobactin to prepare ten analogues and the bioactivity of them was investigated.The SAR studies revealed that Ile_(6) was tolerable for both thioamidation and fiuoridation,while Ser_(7) was identified as the most tolerable site for thioamidation.Analogue 1a demonstrated comparable or slightly improved antibacterial activity,superior protease stability compared to Leu_(10)-teixobactin,while not exhibiting obvious cytotoxicity against mammalian cells.展开更多
Lithium iron phosphate(LFP)has found many applications in the field of electric vehicles and energy storage systems.However,the increasing volume of end-of-life LFP batteries poses an urgent challenge in terms of envi...Lithium iron phosphate(LFP)has found many applications in the field of electric vehicles and energy storage systems.However,the increasing volume of end-of-life LFP batteries poses an urgent challenge in terms of environmental sustainability and resource management.Therefore,the development and implementation of efficient LFP battery recycling methods are crucial to address these challenges.This article presents a novel,comprehensive evaluation framework for comparing different lithium iron phosphate relithiation techniques.The framework includes three main sets of criteria:direct production cost,electrochemical performance,and environmental impact.Each criterion is scored on a scale of 0–100,with higher scores indicating better performance.The direct production cost is rated based on material costs,energy consumption,key equipment costs,process duration and space requirements.Electrochemical performance is assessed by rate capability and cycle stability.Environmental impact is assessed based on CO_(2)emissions.The framework provides a standardized technique for researchers and industry professionals to objectively compare relithiation methods,facilitating the identification of the most promising approaches for further development and scale-up.The total average score across the three criterion groups for electrochemical,chemical,and hydrothermal relithiation methods was approximately 60 points,while sintering scored 39 points,making it the least attractive relithiation technique.Combining approaches outlined in publications with scores exceeding 60,a relithiation scheme was proposed to achieve optimal electrochemical performance with minimal resource consumption and environmental impact.The results demonstrate the framework’s applicability and highlight areas for future research and optimization in lithium iron phosphate cathode recycling.展开更多
Stromal vascular fraction(SVF)therapy is a promising regenerative medicine strategy derived from adipose tissue,containing a heterogeneous mix of cells,including adipose-derived stem,endothelial,and immune cells.Despi...Stromal vascular fraction(SVF)therapy is a promising regenerative medicine strategy derived from adipose tissue,containing a heterogeneous mix of cells,including adipose-derived stem,endothelial,and immune cells.Despite its potential in treating conditions like osteoarthritis,chronic wounds,and myo-cardial ischemia,significant challenges impede its clinical translation.Key obstacles include biological variability in SVF composition,unclear mechanisms of action,regulatory ambiguities,and the technical difficulty of ensuring stan-dardized and scalable isolation methods.Furthermore,patient-specific factors,ethical concerns,and the need for comprehensive efficacy assessment complicate clinical application.Addressing these challenges requires advancements in technology,regulatory flexibility,interdisciplinary collaboration,and person-alized therapeutic approaches.Innovations such as automated isolation systems,advanced biomaterials,and CRISPR-based gene editing are potential solutions to improve the therapeutic reliability of SVF.A structured roadmap,including preclinical research,regulatory approval,and post-market surveillance,is proposed to advance SVF therapies from the laboratory to clinical practice.Future directions should focus on large-scale clinical trials,biomarker development,real-world evidence generation,and standardization of protocols to enhance the safety,efficacy,and accessibility of SVF,ultimately realizing its potential as a versatile therapeutic in regenerative medicine.展开更多
BACKGROUND In intertrochanteric fractures,the positive medial cortex support reduction is considered to provide a non-anatomical buttress that helps in controlled collapse.AIM To analyze the concept of medial cortical...BACKGROUND In intertrochanteric fractures,the positive medial cortex support reduction is considered to provide a non-anatomical buttress that helps in controlled collapse.AIM To analyze the concept of medial cortical reduction(MCR)and its clinical and radiological association in geriatric intertrochanteric femur fractures.METHODS Geriatric patients who presented with AO/OTA 31A1 and 31A2 femur fractures and treated with proximal femoral nailing between July 2021 and June 2023 were include in this prospective cohort study.Based on the degree of MCR,they were divided into positive,neutral,or negative MCR groups.The demographic baseline characteristics,postoperative radiographic femoral neck-shaft angle and neck length were analyzed at 6,12 and 24 weeks post-surgery.Functional outcomes such as modified Harris Hip Score(HHS)and time to full-weight bearing were also analyzed.RESULTS 47 patients(Male:Famale 35:12)with mean age of 65.8±4.2 years were included in this study.Twenty-two cases had neutral support,nine had negative support,and sixteen had positive support in the medial cortex postoperatively.Baseline characteristics of the three groups were comparable.No significant differences were found in the femur neck length and femur neck-shaft angle changes post-surgery between the groups.The modified HHS was not found to be significant between the groups(P=0.883)as that of the time to full weight bearing(P=0.789).CONCLUSION The type of reduction achieved based on medial cortical alignment does not affect the femur neck length shortening or varus collapse.Future randomized controlled trials are needed to validate the findings noted in the study.展开更多
BACKGROUND Osteoarthritis(OA)of the knee is a prevalent degenerative joint disease that significantly impairs quality of life and functional mobility.Emerging regenerative therapies,such as stromal vascular fraction(S...BACKGROUND Osteoarthritis(OA)of the knee is a prevalent degenerative joint disease that significantly impairs quality of life and functional mobility.Emerging regenerative therapies,such as stromal vascular fraction(SVF)and nanofat,have demonstrated potential in cartilage repair and symptom alleviation.However,comparative clinical evidence evaluating their efficacy and safety remains limited.AIM To compare the clinical outcomes of SVF vs nanofat therapy in patients with primary knee OA.METHODS Conducted at Mother Cell Regenerative Centre,Trichy,over 18 months(June 2025 to December 2026),the study will enroll 30 patients,randomly assigned to two groups of 15 each.Both interventions will be administered as a single intra-articular injection under sterile conditions,with cell viability(>85%)confirmed by a standardized assay.Group A will receive autologous SVF injections,while Group B will receive autologous nanofat injections.The primary outcome measure is the change in pain scores at 12 months using the visual analog scale(VAS).Secondary outcomes include functional improvement assessed by Osteoarthritis Outcome Score(KOOS),Western Ontario and McMaster Universities(WOMAC),and International Knee Documentation Committee(IKDC)scores,cartilage regeneration evaluated via magnetic resonance imaging with colour coded mapping of the cartilage volume(MR cartigram),and monitoring of adverse events.RESULTS This study aims to evaluate pain reduction at 12 months post-injection,using the VAS as the primary outcome.Secondary outcomes include functional improvement(KOOS,WOMAC,IKDC),cartilage regeneration(T2 cartigram),adverse event incidence,patient satisfaction(standardized questionnaires,Likert scale),and quality of life(EQ-5D).Ethical considerations follow the Declaration of Helsinki and Good Clinical Practice,with IRB approval and participant informed consent ensured.Confidentiality and data security comply with regulations,and a Data Safety Monitoring Board oversees trial safety.Results will be shared via peer-reviewed journals,presentations at international orthopedic conferences,and detailed summaries for stakeholders and participants.The trial is registered under CTRI/2024/03/064076.Findings emphasize patient-centered advancements in knee osteoarthritis management.CONCLUSION This trial evaluates the efficacy and safety of SVF and nanofat therapies in knee OA,addressing a significant evidence gap.It employs robust methods to enhance cartilage repair and patient quality of life.Future research should standardize dosages,protocols,and injection techniques,explore autologous/allogenic preparations,and advance radiological tools,broadening accessibility and clinical applications.展开更多
BACKGROUND Orthopedic training,one of the most useful but under-represented specialties in undergraduate medical curricula,has some difficulties in clinical teaching.AIM To determine if simulation-based learning(SBL)w...BACKGROUND Orthopedic training,one of the most useful but under-represented specialties in undergraduate medical curricula,has some difficulties in clinical teaching.AIM To determine if simulation-based learning(SBL)was effective in enhancing procedural accuracy,skill confidence,and knowledge recall in final-year medical students.METHODS This was a cross-sectional observational study performed in the Department of Orthopaedics in a tertiary care teaching hospital with simulation training facilities.The trial was conducted over 2 months(January 2025-February 2025)and was designed to determine the effect of SBL on procedural skills,knowledge retention,and self-perceived confidence in a group of final-year medical students.The inclusion criterion was undergraduate medical students in their final year who had clinical postings in orthopedics.Convenience sampling was used to recruit 106 students.The sample size was estimated to achieve a post-training 50%improvement ratio in procedural accuracy,a confidence interval of 95%,and an absolute precision of 10%.The simulation sessions included exercises with synthetic bone models,cast equipment,and procedural packs[orthopedic skills:(1)Closed fracture reduction;(2)Application of plaster;and(3)Traction].Each session consisted of an instructor-led demonstration,practice under supervision,and immediate feedback.RESULTS In our study involving 106 final-year medical students,SBL significantly improved procedural accuracy with scores rising from 62.5%to 84.9%(P<0.001).Knowledge retention also improved markedly with post-test scores increasing from 63.4%to 78.2%(P<0.001).Self-reported confidence levels showed a substantial gain,increasing from 4.6 to 8.2 on a 10-point scale.Prior simulation exposure and academic performance≥75%were significantly associated with higher post-training accuracy.Gender had no significant influence on outcomes.CONCLUSION The current study attested to the value of SBL in the enhancement of procedural skills,knowledge retention,and self-confidence of final-year medical students in orthopedics.展开更多
In regenerative medicine,the isolation of mesenchymal stromal cells(MSCs)from the adipose tissue’s stromal vascular fraction(SVF)is a critical area of study.Our review meticulously examines the isolation process of M...In regenerative medicine,the isolation of mesenchymal stromal cells(MSCs)from the adipose tissue’s stromal vascular fraction(SVF)is a critical area of study.Our review meticulously examines the isolation process of MSCs,starting with the extraction of adipose tissue.The choice of liposuction technique,anatomical site,and immediate processing are essential to maintain cell functionality.We delve into the intricacies of enzymatic digestion,emphasizing the fine-tuning of enzyme concentrations to maximize cell yield while preventing harm.The review then outlines the filtration and centrifugation techniques necessary for isolating a purified SVF,alongside cell viability assessments like flow cytometry,which are vital for confirming the efficacy of the isolated MSCs.We discuss the advantages and drawbacks of using autologous vs allogeneic SVF sources,touching upon immunocompatibility and logistical considerations,as well as the variability inherent in donor-derived cells.Anesthesia choices,the selection between hypo-dermic needles vs liposuction cannulas,and the role of adipose tissue lysers in achieving cellular dissociation are evaluated for their impact on SVF isolation.Centrifugation protocols are also analyzed for their part in ensuring the integrity of the SVF.The necessity for standardized MSC isolation protocols is highlighted,promoting reproducibility and successful clinical application.We encourage ongoing research to deepen the understanding of MSC biology and therapeutic action,aiming to further the field of regenerative medicine.The review concludes with a call for rigorous research,interdisciplinary collaboration,and strict adherence to ethical and regulatory standards to safeguard patient safety and optimize treatment outcomes with MSCs.展开更多
Purpose–This study aims to propose a cooperative adhesion control method for trains with multiple motors electric locomotives.The method is intended to optimize the output torque of each motor,maximize the utilizatio...Purpose–This study aims to propose a cooperative adhesion control method for trains with multiple motors electric locomotives.The method is intended to optimize the output torque of each motor,maximize the utilization of train adhesion within the total torque command,reduce the train skidding/sliding phenomenon and achieve optimal adhesion utilization for each axle,thus realizing the optimal allocation of the multi-motor electric locomotives.Design/methodology/approach–In this study,a model predictive control(MPC)-based cooperative maximum adhesion tracking control method for multi-motor electric locomotives is presented.Firstly,train traction system with multiple motors is constructed in accordance with Newton’s second law.These equations include the train dynamics equations,the axle dynamics equations,and the wheel-rail adhesion coefficient equations.Then,a new MPC-based multi-axle adhesion co-optimization method is put forward.This method calculates the optimal output torque through real-time iteration based on the known reference slip speed to achieve multi-axle co-optimization under different circumstances.Findings–This paper presents a MPC system designed for the cooperative control of multi-axle adhesion.The results indicate that the proposed control system is able to optimize the adhesion of multiple axles under numerous different conditions and achieve the optimal power distribution based on the reduction of train skidding/sliding.Originality/value–This study presents a novel cooperative adhesion tracking control scheme.It is designed for multi-motor electric locomotives,which has rarely been studied before.And simulations are carried out in different conditions,including variable surfaces and motor failing.展开更多
Knee osteoarthritis(OA)is a debilitating condition with limited long-term treatment options.The therapeutic potential of mesenchymal stem cells(MSCs),particularly those derived from bone marrow aspirate concentrate,ha...Knee osteoarthritis(OA)is a debilitating condition with limited long-term treatment options.The therapeutic potential of mesenchymal stem cells(MSCs),particularly those derived from bone marrow aspirate concentrate,has garnered attention for cartilage repair in OA.While the iliac crest is the traditional site for bone marrow harvesting(BMH),associated morbidity has prompted the exploration of alternative sites such as the proximal tibia,distal femur,and proximal humerus.This paper reviews the impact of different harvesting sites on mesenchymal stem cell(MSC)yield,viability,and regenerative potential,emphasizing their relevance in knee OA treatment.The iliac crest consistently offers the highest MSC yield,but alternative sites within the surgical field of knee procedures offer comparable MSC characteristics with reduced morbidity.The integration of harvesting techniques into existing knee surgeries,such as total knee arthroplasty,provides a less invasive approach while maintaining thera-peutic efficacy.However,variability in MSC yield from these alternative sites underscores the need for further research to standardize techniques and optimize clinical outcomes.Future directions include large-scale comparative studies,advanced characterization of MSCs,and the development of personalized harvesting strategies.Ultimately,the findings suggest that optimizing the site of BMH can significantly influence the quality of MSC-based therapies for knee OA,enhancing their clinical utility and patient outcomes.展开更多
The critical role of patient-reported outcome measures(PROMs)in enhancing clinical decision-making and promoting patient-centered care has gained a profound significance in scientific research.PROMs encapsulate a pati...The critical role of patient-reported outcome measures(PROMs)in enhancing clinical decision-making and promoting patient-centered care has gained a profound significance in scientific research.PROMs encapsulate a patient's health status directly from their perspective,encompassing various domains such as symptom severity,functional status,and overall quality of life.By integrating PROMs into routine clinical practice and research,healthcare providers can achieve a more nuanced understanding of patient experiences and tailor treatments accordingly.The deployment of PROMs supports dynamic patient-provider interactions,fostering better patient engagement and adherence to tre-atment plans.Moreover,PROMs are pivotal in clinical settings for monitoring disease progression and treatment efficacy,particularly in chronic and mental health conditions.However,challenges in implementing PROMs include data collection and management,integration into existing health systems,and acceptance by patients and providers.Overcoming these barriers necessitates technological advancements,policy development,and continuous education to enhance the acceptability and effectiveness of PROMs.The paper concludes with recommendations for future research and policy-making aimed at optimizing the use and impact of PROMs across healthcare settings.展开更多
In the intricate landscape of healthcare,vicarious liability looms large,shaping the responsibilities and actions of healthcare practitioners and administrators alike.Illustrated by a poignant scenario of a medication...In the intricate landscape of healthcare,vicarious liability looms large,shaping the responsibilities and actions of healthcare practitioners and administrators alike.Illustrated by a poignant scenario of a medication error,this article navigates the complexities of vicarious liability in healthcare.It explains the legal basis and ramifications of this theory,emphasizing its importance in fostering responsibility,protecting patient welfare,and easing access to justice.The paper explores the practical effects of vicarious responsibility on day-to-day operations,leadership practices,and decision-making processes via the eyes of senior consultants,junior doctors,and hospital administrators.Through comprehensive insights and real-world examples,it underscores the imperative of fostering a culture of accountability,communication,and quality care to navigate the intricate web of liabilities inherent in modern healthcare.展开更多
The minimal clinically important difference(MCID)represents a pivotal metric in bridging the gap between statistical significance and clinical relevance,addressing the direct impact of medical interventions from the p...The minimal clinically important difference(MCID)represents a pivotal metric in bridging the gap between statistical significance and clinical relevance,addressing the direct impact of medical interventions from the patient's perspective.This comprehensive review analyzes the evolution,applications,and challenges of MCID across medical specialties,emphasizing its necessity in ensuring that clinical outcomes not only demonstrate statistical significance but also offer genuine clinical utility that aligns with patient expectations and needs.We discuss the evolution of MCID since its inception in the 1980s,its current applications across various medical specialties,and the methodologies used in its calculation,highlighting both anchor-based and distribution-based approaches.Furthermore,the paper delves into the challenges associated with the application of MCID,such as methodological variability and the interpretation difficulties that arise in clinical settings.Recommendations for the future include standardizing MCID calculation methods,enhancing patient involvement in setting MCID thresholds,and extending research to incorporate diverse global perspectives.These steps are critical to refining the role of MCID in patient-centered healthcare,addressing existing gaps in methodology and interpretation,and ensuring that medical interventions lead to significant,patient-perceived improvements.展开更多
Rice false smut(RFS),caused by the ascomycete fungus Ustilaginoidea virens(Cooke)Takahashi,is a notable panicle disease of rice.Mycoviruses represent a category of viruses capable of infecting various fungal groups,ga...Rice false smut(RFS),caused by the ascomycete fungus Ustilaginoidea virens(Cooke)Takahashi,is a notable panicle disease of rice.Mycoviruses represent a category of viruses capable of infecting various fungal groups,garnering interest for their potential application in the biological control of plant fungal diseases.We identified a novel positive-sense single-stranded RNA virus,named Ustilaginoidea virens mitovirus 1(UvMV1),from an abnormal U.virens strain Uv488.The complete genome sequence of UvMV1 is 2474 nt in length and contains a large open reading frame encoding RNA-dependent RNA polymerase.UvMV1 is classified as a new member of the genus Unuamitovirus in the family Mitoviridae based on phylogenetic analysis.It is effectively transmitted vertically through conidia of strain Uv488 and horizontally through hyphal fusion between vegetatively compatible individuals with an 80%transmission efficiency.We further demonstrated that UvMV1 significantly influenced conidiation,colony morphology,growth rate,secondary metabolite production,and the response to environmental stress in U.virens.Furthermore,qRT-PCR results aimed at detecting the expression levels of autophagy-related genes suggested that UvMV1 infection had the potential to induce autophagy in U.virens.Activation or inhibition of autophagy through chemical experiments demonstrated that UvMV1 enhances viral titers by inducing autophagy in U.virens,while inhibition of autophagy results in decreased UvMV1 titers.Transcriptome analyses consistently demonstrated that UvMV1 regulated the expression of genes associated with secondary metabolism,mycelial growth,virulence,and the mitogen-activated protein kinase signaling pathway,thereby influencing both the antiviral response and the virulence of U.virens.The data presented collectively identified a novel mycovirus responsible for inducing growth abnormalities in U.virens,and elucidated the regulatory mechanisms during mycovirus-host fungus interactions,particularly the autophagy pathway.These findings offer new perspectives and potential control strategies for managing RFS in agricultural settings.展开更多
OBJECTIVE:To screen the target population with symptomatic bradyarrhythmia for pharmacotherapy.METHODS:This study use database from clinical trial that use Yuanjiang decoction(元姜方),a traditional Chinese medicinal p...OBJECTIVE:To screen the target population with symptomatic bradyarrhythmia for pharmacotherapy.METHODS:This study use database from clinical trial that use Yuanjiang decoction(元姜方),a traditional Chinese medicinal prescription.Eligible participants were recruited and treated with Yuanjiang decoction(composed of 6 Chinese herbal medicines),200 m L twice daily.Cross-contingency analysis,logistic regression analysis,interaction analysis,discriminant analysis and 10-fold cross validation methods were used to establish discriminant model.RESULTS:This study concluded that the clinical treatment of bradyarrhythmia has a clear effect.Low minimum heart rate,high maximum heart rate are risk factors that affect the efficacy.Patients with only one comorbid disease did not significantly affect the efficacy,but patient with two or more diseases of coronary heart disease,hypertension,paroxysmal atrial fibrillation,premature ventricular contraction and premature atrial contraction at the same time did not have a good effect.Using the discriminant analysis method to establish a efficacy prediction model,y=0.07X1+0.16X2-0.65X3-1.12X4-0.71X5-0.75X6-0.91X7(X1=24 h mean heart rate,X2=minimum heart rate,X3=coronary heart disease,X4=paroxysmal atrial fibrillation,X5=premature ventricular contraction,X6=sinus block,X7=atrioventricular block).CONCLUSION:Our model based on the clinical features of patients with bradyarrhythmia.Should be useful aid for predicting pharmacotherapy response and could screen the optimal pharmacotherapy target.展开更多
The intricate interplay between natural compounds like curcumin and the gut microbiome has gained significant attention in recent years due to their potential therapeutic implications in various health conditions.Curc...The intricate interplay between natural compounds like curcumin and the gut microbiome has gained significant attention in recent years due to their potential therapeutic implications in various health conditions.Curcumin,a polyphenolic compound derived from turmeric,exhibits diverse pharmacological properties,including anti-inflammatory,antioxidant,and anticancer effects.Understanding how curcumin modulates gut microbiota composition and function is crucial for elucidating its therapeutic mechanisms.This review examines the current literature on the interactions between curcumin and the gut microbiome.A systematic search of relevant databases was conducted to identify studies investigating the effects of curcumin on gut microbial diversity and abundance.Key findings from studies exploring curcumin's efficacy in neurological disorders,gastrointestinal diseases,and metabolic dysfunction are synthesized and discussed.Studies have demonstrated that curcumin supplementation can modulate gut microbiota composition and function,leading to beneficial effects on gut health and homeostasis.Mechanisms underlying curcumin's therapeutic effects include immune modulation,neuroprotection,and inflammation regulation.However,challenges such as poor bioavailability and safety concerns remain significant hurdles to overcome.The interactions between curcumin and the gut microbiome hold promise for therapeutic interventions in a diverse range of health conditions.Further research is needed to optimize curcumin formulations,improve bioavailability,and address safety concerns.展开更多
BACKGROUND Current osteoarthritis(OA)treatments focus on symptom relief without addressing the underlying disease process.In regenerative medicine,current treatments have limitations.In regenerative medicine,more rese...BACKGROUND Current osteoarthritis(OA)treatments focus on symptom relief without addressing the underlying disease process.In regenerative medicine,current treatments have limitations.In regenerative medicine,more research is needed for intra-articular stromal vascular fraction(SVF)injections in OA,including dosage optimization,long-term efficacy,safety,comparisons with other treatments,and mechanism exploration.AIM To compare the efficacy of intra-articular SVF with corticosteroid(ICS)injections in patients with primary knee OA.METHODS The study included 50 patients with Kellgren-Lawrence grades II and III OA.Patients were randomly assigned(1:1)to receive either a single intra-articular SVF injection(group A)or a single intra-articular ICS(triamcinolone)(group B)injection.Patients were followed up at 1,3,6,12,and 24 months.Visual analog score(VAS)and International Knee Documentation Committee(IKDC)scores were administered before the procedure and at all followups.The safety of SVF in terms of adverse and severe adverse events was recorded.Statistical analysis was performed with SPSS Version 26.0,IBM Corp,Chicago,IL,United States.RESULTS Both groups had similar demographics and baseline clinical characteristics.Follow-up showed minor patient loss,resulting in 23 and 24 in groups A and B respectively.Group A experienced a notable reduction in pain,with VAS scores decreasing from 7.7 to 2.4 over 24 months,compared to a minor reduction from 7.8 to 6.2 in Group B.This difference in pain reduction in group A was statistically significant from the third month onwards.Additionally,Group A showed significant improvements in knee functionality,with IKDC scores rising from 33.4 to 83.10,whereas Group B saw a modest increase from 36.7 to 45.16.The improvement in Group A was statistically significant from 6 months and maintained through 24 months.CONCLUSION Our study demonstrated that intra-articular administration of SVF can lead to reduced pain and improved knee function in patients with primary knee OA.More adequately powered,multi-center,double-blinded,randomised clinical trials with longer follow-ups are needed to further establish safety and justify its clinical use.展开更多
During the 2nd Indochina War which started in 1959, the United States Central Intelligence Agency (CIA), Air America, and the Air Force waged a secret and unconventional air war in Laos from Udorn Air Force base locat...During the 2nd Indochina War which started in 1959, the United States Central Intelligence Agency (CIA), Air America, and the Air Force waged a secret and unconventional air war in Laos from Udorn Air Force base located in Thailand and across the Mekong River from Vientiane, Laos. Starting in 1961, four years before the official start of the American-Vietnam War, Agent Blue, the arsenic-based herbicide used to kill rice and other food crops, was used extensively in Laos, Vietnam and to a lesser extent in Cambodia. During the secret 2nd Indochina War and the Vietnam Civil War the public knew little about the use of Agent Blue. After the official start of the American-Vietnam War in 1965, the United States media news reports, about chemical warfare were dominated by the story of Agent Orange and its devastating impacts. The public knew very little about the previous use of Agent Blue in both wars. The first known media pick up of the Agent Blue (arsenic based) and Agent Pink, Agent Green, and Agent Purple (all three contain 2, 4, 5-T and unknown amounts of dioxin TCDD) was in May of 1964. Jim G. Lucas, a Scripps-Howard staff reporter submitted an article that was published as an editorial in Washington Post on May 26, 1964. The next news reference to this chemical weapon was a Letter to the Editor published in the New York Times titled “Agent Blue” in Vietnam by Arthur H. Westing in (1971). The use of herbicides, including Agent Blue in Laos during the 2nd Indochina War, was kept a secret until 1982, when a draft of Buckingham’s study of Operation Ranch Hand was made public. Much about the U.S. war effort in Laos is still classified. In a 2014 issue of the VVA Veteran magazine, Loana Hoylman published an article on “Today’s Blue Arsenic in the Environment”. The first refereed journal article on this topic, “The Fate of Agent Blue, the Arsenic-Based Herbicide, Used in South Vietnam during the Vietnam War” was published in 2020 in the Open Journal of Soil Science by Kenneth R. Olson and Larry Cihacek. In 2021 the Asia Times (print) and VietnamVeteranNews (radio podcast) picked up the Agent Blue story. During the early 2020s, Olson published six additional refereed journal articles on Agent Blue, cacodylic acid, and arsenic. The primary objective is to determine why no major news organization in the United States, including the New York Times and Washington Post, have never investigated Agent Blue use during the 2nd Indochina and Vietnam wars? Why did the use of Agent Blue story, used to destroy Laotian and South Vietnamese civilian food (rice) sources and production sites, received only very limited coverage by US print media news organizations during the last 64 years?展开更多
BACKGROUND Adipose-derived stem cells(ADSCs)and the stromal vascular fraction(SVF)have garnered substantial interest in regenerative medicine due to their potential to treat a wide range of conditions.Traditional enzy...BACKGROUND Adipose-derived stem cells(ADSCs)and the stromal vascular fraction(SVF)have garnered substantial interest in regenerative medicine due to their potential to treat a wide range of conditions.Traditional enzymatic methods for isolating these cells face challenges such as high costs,lengthy processing time,and regulatory complexities.AIM This systematic review aimed to assess the efficacy and practicality of nonenzymatic,mechanical methods for isolating SVF and ADSCs,comparing these to conventional enzymatic approaches.METHODS Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines,a comprehensive literature search was conducted across multiple databases.Studies were selected based on inclusion criteria focused on non-enzymatic isolation methods for SVF and ADSCs from adipose tissue.The risk of bias was assessed,and a qualitative synthesis of findings was performed due to the methodological heterogeneity of the included studies.RESULTS Nineteen studies met the inclusion criteria,highlighting various mechanical techniques such as centrifugation,vortexing,and ultrasonic cavitation.The review identified significant variability in cell yield and viability,and the integrity of isolated cells across different non-enzymatic methods compared to enzymatic procedures.Despite some advantages of mechanical methods,including reduced processing time and avoidance of enzymatic reagents,the evidence suggests a need for optimization to match the cell quality and therapeutic efficacy achievable with enzymatic isolation.CONCLUSION Non-enzymatic,mechanical methods offer a promising alternative to enzymatic isolation of SVF and ADSCs,potentially simplifying the isolation process and reducing regulatory hurdles.However,further research is necessary to standardize these techniques and ensure consistent,high-quality cell yields for clinical applications.The development of efficient,safe,and reproducible non-enzymatic isolation methods could significantly advance the field of regenerative medicine.展开更多
OBJECTIVE:To establish a standardized framework encompassing the precise locations,manipulations,functions and indications of specific acupoints in the field of paediatric Tuina.METHODS:The development of consensus in...OBJECTIVE:To establish a standardized framework encompassing the precise locations,manipulations,functions and indications of specific acupoints in the field of paediatric Tuina.METHODS:The development of consensus involved three distinct stages.Initially,a list of paediatric Tuina specific acupoints was compiled based on an extensive literature review,which was subsequently supplemented through expert interviews.In the second stage,the Delphi method was employed to assess the significance of acupoint locations,manipulations,functions,and indications.In situations where the questionnaire survey failed to yield agreement or when the experts held reservations,the nominal group approach was utilized during the expert consensus meeting.The final version of the technical standardized material was ultimately determined during an expert consensus conference.After undergoing external peer review and evaluation,the completed draft was prepared for public dissemination RESULTS:The comprehensive list identified a total of 66 specific acupoints.The location and manipulation questionnaire consisted of 156 items based on the literature database,while the function and indication questionnaire contained 116 items.Two rounds of Delphi surveys were conducted for the location and manipulation category,and another two rounds of Delphi surveys were conducted for the function and indication category.During the experts consensus meeting The panel of experts conducted in-depth discussions on 61 questions,resulting in the formulation of technical guidelines for the locations,manipulations,functions,and indications of 64 paediatric Tuina acupoints.Subsequently,the research team compiled and edited the draft of the technical guidelines for acupoints of paediatric Tuina,which was finalized after external review and feedback.CONCLUSION:This study successfully established the recognized technical standards for practitioners of paediatric Tuina,thereby standardizing clinical practices and providing a foundation setting the framework for future research.The guidelines offer theoretical insights and recommendations for conducting clinical studies comparing different acupoint sites,as well as modifying or enhancing treatment regimens.展开更多
文摘Graphical abstracts(GAs)are emerging as a pivotal tool in medical literature,enhancing the dissemination and comprehension of complex clinical data through visual summaries.This editorial highlights the significant advantages of GAs,including improved clarity,increased reader engagement,and enhanced visibility of research findings.By transforming intricate scientific data into accessible visual formats,these abstracts facilitate quick and effective knowledge transfer,crucial in clinical decision-making and patient care.However,challenges such as potential data misrepresentation due to oversimplification,the skill gap in graphic design among researchers,and the lack of standardized creation guidelines pose barriers to their widespread adoption.Additionally,while software such as Adobe Illustrator,BioRender,and Canva are commonly employed to create these visuals,not all researchers may be proficient in their use.To address these issues,we recommend that academic journals establish clear guidelines and provide necessary design training to researchers.This proactive approach will ensure the creation of high-quality GAs,promote their standardization,and expand their use in clinical reporting,ultimately benefiting the medical community and improving healthcare outcomes.
基金supported by the National Natural Science Foundation of China(No.21977111)the Natural Science Foundation of Guangdong Province(No.2023A1515011765)Shenzhen Science and Technology Program(Nos.JCYJ20220818101404010,JCYJ20220818100412028)。
文摘Two thioamino acids and four fiuorinated amino acids were employed to substitute either partially or entirely the Ile_(2),Ser_(3),Ile_(6),and Ser_(7) residues of Leu_(10)-teixobactin to prepare ten analogues and the bioactivity of them was investigated.The SAR studies revealed that Ile_(6) was tolerable for both thioamidation and fiuoridation,while Ser_(7) was identified as the most tolerable site for thioamidation.Analogue 1a demonstrated comparable or slightly improved antibacterial activity,superior protease stability compared to Leu_(10)-teixobactin,while not exhibiting obvious cytotoxicity against mammalian cells.
基金state assignments of Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry,Russian Academy of Sciences(No.124013000692-4 and 122112100037-4).
文摘Lithium iron phosphate(LFP)has found many applications in the field of electric vehicles and energy storage systems.However,the increasing volume of end-of-life LFP batteries poses an urgent challenge in terms of environmental sustainability and resource management.Therefore,the development and implementation of efficient LFP battery recycling methods are crucial to address these challenges.This article presents a novel,comprehensive evaluation framework for comparing different lithium iron phosphate relithiation techniques.The framework includes three main sets of criteria:direct production cost,electrochemical performance,and environmental impact.Each criterion is scored on a scale of 0–100,with higher scores indicating better performance.The direct production cost is rated based on material costs,energy consumption,key equipment costs,process duration and space requirements.Electrochemical performance is assessed by rate capability and cycle stability.Environmental impact is assessed based on CO_(2)emissions.The framework provides a standardized technique for researchers and industry professionals to objectively compare relithiation methods,facilitating the identification of the most promising approaches for further development and scale-up.The total average score across the three criterion groups for electrochemical,chemical,and hydrothermal relithiation methods was approximately 60 points,while sintering scored 39 points,making it the least attractive relithiation technique.Combining approaches outlined in publications with scores exceeding 60,a relithiation scheme was proposed to achieve optimal electrochemical performance with minimal resource consumption and environmental impact.The results demonstrate the framework’s applicability and highlight areas for future research and optimization in lithium iron phosphate cathode recycling.
文摘Stromal vascular fraction(SVF)therapy is a promising regenerative medicine strategy derived from adipose tissue,containing a heterogeneous mix of cells,including adipose-derived stem,endothelial,and immune cells.Despite its potential in treating conditions like osteoarthritis,chronic wounds,and myo-cardial ischemia,significant challenges impede its clinical translation.Key obstacles include biological variability in SVF composition,unclear mechanisms of action,regulatory ambiguities,and the technical difficulty of ensuring stan-dardized and scalable isolation methods.Furthermore,patient-specific factors,ethical concerns,and the need for comprehensive efficacy assessment complicate clinical application.Addressing these challenges requires advancements in technology,regulatory flexibility,interdisciplinary collaboration,and person-alized therapeutic approaches.Innovations such as automated isolation systems,advanced biomaterials,and CRISPR-based gene editing are potential solutions to improve the therapeutic reliability of SVF.A structured roadmap,including preclinical research,regulatory approval,and post-market surveillance,is proposed to advance SVF therapies from the laboratory to clinical practice.Future directions should focus on large-scale clinical trials,biomarker development,real-world evidence generation,and standardization of protocols to enhance the safety,efficacy,and accessibility of SVF,ultimately realizing its potential as a versatile therapeutic in regenerative medicine.
文摘BACKGROUND In intertrochanteric fractures,the positive medial cortex support reduction is considered to provide a non-anatomical buttress that helps in controlled collapse.AIM To analyze the concept of medial cortical reduction(MCR)and its clinical and radiological association in geriatric intertrochanteric femur fractures.METHODS Geriatric patients who presented with AO/OTA 31A1 and 31A2 femur fractures and treated with proximal femoral nailing between July 2021 and June 2023 were include in this prospective cohort study.Based on the degree of MCR,they were divided into positive,neutral,or negative MCR groups.The demographic baseline characteristics,postoperative radiographic femoral neck-shaft angle and neck length were analyzed at 6,12 and 24 weeks post-surgery.Functional outcomes such as modified Harris Hip Score(HHS)and time to full-weight bearing were also analyzed.RESULTS 47 patients(Male:Famale 35:12)with mean age of 65.8±4.2 years were included in this study.Twenty-two cases had neutral support,nine had negative support,and sixteen had positive support in the medial cortex postoperatively.Baseline characteristics of the three groups were comparable.No significant differences were found in the femur neck length and femur neck-shaft angle changes post-surgery between the groups.The modified HHS was not found to be significant between the groups(P=0.883)as that of the time to full weight bearing(P=0.789).CONCLUSION The type of reduction achieved based on medial cortical alignment does not affect the femur neck length shortening or varus collapse.Future randomized controlled trials are needed to validate the findings noted in the study.
文摘BACKGROUND Osteoarthritis(OA)of the knee is a prevalent degenerative joint disease that significantly impairs quality of life and functional mobility.Emerging regenerative therapies,such as stromal vascular fraction(SVF)and nanofat,have demonstrated potential in cartilage repair and symptom alleviation.However,comparative clinical evidence evaluating their efficacy and safety remains limited.AIM To compare the clinical outcomes of SVF vs nanofat therapy in patients with primary knee OA.METHODS Conducted at Mother Cell Regenerative Centre,Trichy,over 18 months(June 2025 to December 2026),the study will enroll 30 patients,randomly assigned to two groups of 15 each.Both interventions will be administered as a single intra-articular injection under sterile conditions,with cell viability(>85%)confirmed by a standardized assay.Group A will receive autologous SVF injections,while Group B will receive autologous nanofat injections.The primary outcome measure is the change in pain scores at 12 months using the visual analog scale(VAS).Secondary outcomes include functional improvement assessed by Osteoarthritis Outcome Score(KOOS),Western Ontario and McMaster Universities(WOMAC),and International Knee Documentation Committee(IKDC)scores,cartilage regeneration evaluated via magnetic resonance imaging with colour coded mapping of the cartilage volume(MR cartigram),and monitoring of adverse events.RESULTS This study aims to evaluate pain reduction at 12 months post-injection,using the VAS as the primary outcome.Secondary outcomes include functional improvement(KOOS,WOMAC,IKDC),cartilage regeneration(T2 cartigram),adverse event incidence,patient satisfaction(standardized questionnaires,Likert scale),and quality of life(EQ-5D).Ethical considerations follow the Declaration of Helsinki and Good Clinical Practice,with IRB approval and participant informed consent ensured.Confidentiality and data security comply with regulations,and a Data Safety Monitoring Board oversees trial safety.Results will be shared via peer-reviewed journals,presentations at international orthopedic conferences,and detailed summaries for stakeholders and participants.The trial is registered under CTRI/2024/03/064076.Findings emphasize patient-centered advancements in knee osteoarthritis management.CONCLUSION This trial evaluates the efficacy and safety of SVF and nanofat therapies in knee OA,addressing a significant evidence gap.It employs robust methods to enhance cartilage repair and patient quality of life.Future research should standardize dosages,protocols,and injection techniques,explore autologous/allogenic preparations,and advance radiological tools,broadening accessibility and clinical applications.
文摘BACKGROUND Orthopedic training,one of the most useful but under-represented specialties in undergraduate medical curricula,has some difficulties in clinical teaching.AIM To determine if simulation-based learning(SBL)was effective in enhancing procedural accuracy,skill confidence,and knowledge recall in final-year medical students.METHODS This was a cross-sectional observational study performed in the Department of Orthopaedics in a tertiary care teaching hospital with simulation training facilities.The trial was conducted over 2 months(January 2025-February 2025)and was designed to determine the effect of SBL on procedural skills,knowledge retention,and self-perceived confidence in a group of final-year medical students.The inclusion criterion was undergraduate medical students in their final year who had clinical postings in orthopedics.Convenience sampling was used to recruit 106 students.The sample size was estimated to achieve a post-training 50%improvement ratio in procedural accuracy,a confidence interval of 95%,and an absolute precision of 10%.The simulation sessions included exercises with synthetic bone models,cast equipment,and procedural packs[orthopedic skills:(1)Closed fracture reduction;(2)Application of plaster;and(3)Traction].Each session consisted of an instructor-led demonstration,practice under supervision,and immediate feedback.RESULTS In our study involving 106 final-year medical students,SBL significantly improved procedural accuracy with scores rising from 62.5%to 84.9%(P<0.001).Knowledge retention also improved markedly with post-test scores increasing from 63.4%to 78.2%(P<0.001).Self-reported confidence levels showed a substantial gain,increasing from 4.6 to 8.2 on a 10-point scale.Prior simulation exposure and academic performance≥75%were significantly associated with higher post-training accuracy.Gender had no significant influence on outcomes.CONCLUSION The current study attested to the value of SBL in the enhancement of procedural skills,knowledge retention,and self-confidence of final-year medical students in orthopedics.
文摘In regenerative medicine,the isolation of mesenchymal stromal cells(MSCs)from the adipose tissue’s stromal vascular fraction(SVF)is a critical area of study.Our review meticulously examines the isolation process of MSCs,starting with the extraction of adipose tissue.The choice of liposuction technique,anatomical site,and immediate processing are essential to maintain cell functionality.We delve into the intricacies of enzymatic digestion,emphasizing the fine-tuning of enzyme concentrations to maximize cell yield while preventing harm.The review then outlines the filtration and centrifugation techniques necessary for isolating a purified SVF,alongside cell viability assessments like flow cytometry,which are vital for confirming the efficacy of the isolated MSCs.We discuss the advantages and drawbacks of using autologous vs allogeneic SVF sources,touching upon immunocompatibility and logistical considerations,as well as the variability inherent in donor-derived cells.Anesthesia choices,the selection between hypo-dermic needles vs liposuction cannulas,and the role of adipose tissue lysers in achieving cellular dissociation are evaluated for their impact on SVF isolation.Centrifugation protocols are also analyzed for their part in ensuring the integrity of the SVF.The necessity for standardized MSC isolation protocols is highlighted,promoting reproducibility and successful clinical application.We encourage ongoing research to deepen the understanding of MSC biology and therapeutic action,aiming to further the field of regenerative medicine.The review concludes with a call for rigorous research,interdisciplinary collaboration,and strict adherence to ethical and regulatory standards to safeguard patient safety and optimize treatment outcomes with MSCs.
基金supported by Scientific Research Projects of China Association of Metros(CAMET-KY-2022039)State Key Laboratory of Traction and Control System of EMU and Locomotive(2023YJ386).
文摘Purpose–This study aims to propose a cooperative adhesion control method for trains with multiple motors electric locomotives.The method is intended to optimize the output torque of each motor,maximize the utilization of train adhesion within the total torque command,reduce the train skidding/sliding phenomenon and achieve optimal adhesion utilization for each axle,thus realizing the optimal allocation of the multi-motor electric locomotives.Design/methodology/approach–In this study,a model predictive control(MPC)-based cooperative maximum adhesion tracking control method for multi-motor electric locomotives is presented.Firstly,train traction system with multiple motors is constructed in accordance with Newton’s second law.These equations include the train dynamics equations,the axle dynamics equations,and the wheel-rail adhesion coefficient equations.Then,a new MPC-based multi-axle adhesion co-optimization method is put forward.This method calculates the optimal output torque through real-time iteration based on the known reference slip speed to achieve multi-axle co-optimization under different circumstances.Findings–This paper presents a MPC system designed for the cooperative control of multi-axle adhesion.The results indicate that the proposed control system is able to optimize the adhesion of multiple axles under numerous different conditions and achieve the optimal power distribution based on the reduction of train skidding/sliding.Originality/value–This study presents a novel cooperative adhesion tracking control scheme.It is designed for multi-motor electric locomotives,which has rarely been studied before.And simulations are carried out in different conditions,including variable surfaces and motor failing.
文摘Knee osteoarthritis(OA)is a debilitating condition with limited long-term treatment options.The therapeutic potential of mesenchymal stem cells(MSCs),particularly those derived from bone marrow aspirate concentrate,has garnered attention for cartilage repair in OA.While the iliac crest is the traditional site for bone marrow harvesting(BMH),associated morbidity has prompted the exploration of alternative sites such as the proximal tibia,distal femur,and proximal humerus.This paper reviews the impact of different harvesting sites on mesenchymal stem cell(MSC)yield,viability,and regenerative potential,emphasizing their relevance in knee OA treatment.The iliac crest consistently offers the highest MSC yield,but alternative sites within the surgical field of knee procedures offer comparable MSC characteristics with reduced morbidity.The integration of harvesting techniques into existing knee surgeries,such as total knee arthroplasty,provides a less invasive approach while maintaining thera-peutic efficacy.However,variability in MSC yield from these alternative sites underscores the need for further research to standardize techniques and optimize clinical outcomes.Future directions include large-scale comparative studies,advanced characterization of MSCs,and the development of personalized harvesting strategies.Ultimately,the findings suggest that optimizing the site of BMH can significantly influence the quality of MSC-based therapies for knee OA,enhancing their clinical utility and patient outcomes.
文摘The critical role of patient-reported outcome measures(PROMs)in enhancing clinical decision-making and promoting patient-centered care has gained a profound significance in scientific research.PROMs encapsulate a patient's health status directly from their perspective,encompassing various domains such as symptom severity,functional status,and overall quality of life.By integrating PROMs into routine clinical practice and research,healthcare providers can achieve a more nuanced understanding of patient experiences and tailor treatments accordingly.The deployment of PROMs supports dynamic patient-provider interactions,fostering better patient engagement and adherence to tre-atment plans.Moreover,PROMs are pivotal in clinical settings for monitoring disease progression and treatment efficacy,particularly in chronic and mental health conditions.However,challenges in implementing PROMs include data collection and management,integration into existing health systems,and acceptance by patients and providers.Overcoming these barriers necessitates technological advancements,policy development,and continuous education to enhance the acceptability and effectiveness of PROMs.The paper concludes with recommendations for future research and policy-making aimed at optimizing the use and impact of PROMs across healthcare settings.
文摘In the intricate landscape of healthcare,vicarious liability looms large,shaping the responsibilities and actions of healthcare practitioners and administrators alike.Illustrated by a poignant scenario of a medication error,this article navigates the complexities of vicarious liability in healthcare.It explains the legal basis and ramifications of this theory,emphasizing its importance in fostering responsibility,protecting patient welfare,and easing access to justice.The paper explores the practical effects of vicarious responsibility on day-to-day operations,leadership practices,and decision-making processes via the eyes of senior consultants,junior doctors,and hospital administrators.Through comprehensive insights and real-world examples,it underscores the imperative of fostering a culture of accountability,communication,and quality care to navigate the intricate web of liabilities inherent in modern healthcare.
文摘The minimal clinically important difference(MCID)represents a pivotal metric in bridging the gap between statistical significance and clinical relevance,addressing the direct impact of medical interventions from the patient's perspective.This comprehensive review analyzes the evolution,applications,and challenges of MCID across medical specialties,emphasizing its necessity in ensuring that clinical outcomes not only demonstrate statistical significance but also offer genuine clinical utility that aligns with patient expectations and needs.We discuss the evolution of MCID since its inception in the 1980s,its current applications across various medical specialties,and the methodologies used in its calculation,highlighting both anchor-based and distribution-based approaches.Furthermore,the paper delves into the challenges associated with the application of MCID,such as methodological variability and the interpretation difficulties that arise in clinical settings.Recommendations for the future include standardizing MCID calculation methods,enhancing patient involvement in setting MCID thresholds,and extending research to incorporate diverse global perspectives.These steps are critical to refining the role of MCID in patient-centered healthcare,addressing existing gaps in methodology and interpretation,and ensuring that medical interventions lead to significant,patient-perceived improvements.
基金supported by the National Natural Science Foundation of China(Grant Nos.32072363 and 32360648).
文摘Rice false smut(RFS),caused by the ascomycete fungus Ustilaginoidea virens(Cooke)Takahashi,is a notable panicle disease of rice.Mycoviruses represent a category of viruses capable of infecting various fungal groups,garnering interest for their potential application in the biological control of plant fungal diseases.We identified a novel positive-sense single-stranded RNA virus,named Ustilaginoidea virens mitovirus 1(UvMV1),from an abnormal U.virens strain Uv488.The complete genome sequence of UvMV1 is 2474 nt in length and contains a large open reading frame encoding RNA-dependent RNA polymerase.UvMV1 is classified as a new member of the genus Unuamitovirus in the family Mitoviridae based on phylogenetic analysis.It is effectively transmitted vertically through conidia of strain Uv488 and horizontally through hyphal fusion between vegetatively compatible individuals with an 80%transmission efficiency.We further demonstrated that UvMV1 significantly influenced conidiation,colony morphology,growth rate,secondary metabolite production,and the response to environmental stress in U.virens.Furthermore,qRT-PCR results aimed at detecting the expression levels of autophagy-related genes suggested that UvMV1 infection had the potential to induce autophagy in U.virens.Activation or inhibition of autophagy through chemical experiments demonstrated that UvMV1 enhances viral titers by inducing autophagy in U.virens,while inhibition of autophagy results in decreased UvMV1 titers.Transcriptome analyses consistently demonstrated that UvMV1 regulated the expression of genes associated with secondary metabolism,mycelial growth,virulence,and the mitogen-activated protein kinase signaling pathway,thereby influencing both the antiviral response and the virulence of U.virens.The data presented collectively identified a novel mycovirus responsible for inducing growth abnormalities in U.virens,and elucidated the regulatory mechanisms during mycovirus-host fungus interactions,particularly the autophagy pathway.These findings offer new perspectives and potential control strategies for managing RFS in agricultural settings.
基金National Natural Science Foundation of China(No.82004352)National Medicine Master's Inheritance Studio Construction Project of the State Administration of Traditional Chinese Medicine(Weng Weiliang Academic Succession Studio)the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ14-YQ-006)。
文摘OBJECTIVE:To screen the target population with symptomatic bradyarrhythmia for pharmacotherapy.METHODS:This study use database from clinical trial that use Yuanjiang decoction(元姜方),a traditional Chinese medicinal prescription.Eligible participants were recruited and treated with Yuanjiang decoction(composed of 6 Chinese herbal medicines),200 m L twice daily.Cross-contingency analysis,logistic regression analysis,interaction analysis,discriminant analysis and 10-fold cross validation methods were used to establish discriminant model.RESULTS:This study concluded that the clinical treatment of bradyarrhythmia has a clear effect.Low minimum heart rate,high maximum heart rate are risk factors that affect the efficacy.Patients with only one comorbid disease did not significantly affect the efficacy,but patient with two or more diseases of coronary heart disease,hypertension,paroxysmal atrial fibrillation,premature ventricular contraction and premature atrial contraction at the same time did not have a good effect.Using the discriminant analysis method to establish a efficacy prediction model,y=0.07X1+0.16X2-0.65X3-1.12X4-0.71X5-0.75X6-0.91X7(X1=24 h mean heart rate,X2=minimum heart rate,X3=coronary heart disease,X4=paroxysmal atrial fibrillation,X5=premature ventricular contraction,X6=sinus block,X7=atrioventricular block).CONCLUSION:Our model based on the clinical features of patients with bradyarrhythmia.Should be useful aid for predicting pharmacotherapy response and could screen the optimal pharmacotherapy target.
文摘The intricate interplay between natural compounds like curcumin and the gut microbiome has gained significant attention in recent years due to their potential therapeutic implications in various health conditions.Curcumin,a polyphenolic compound derived from turmeric,exhibits diverse pharmacological properties,including anti-inflammatory,antioxidant,and anticancer effects.Understanding how curcumin modulates gut microbiota composition and function is crucial for elucidating its therapeutic mechanisms.This review examines the current literature on the interactions between curcumin and the gut microbiome.A systematic search of relevant databases was conducted to identify studies investigating the effects of curcumin on gut microbial diversity and abundance.Key findings from studies exploring curcumin's efficacy in neurological disorders,gastrointestinal diseases,and metabolic dysfunction are synthesized and discussed.Studies have demonstrated that curcumin supplementation can modulate gut microbiota composition and function,leading to beneficial effects on gut health and homeostasis.Mechanisms underlying curcumin's therapeutic effects include immune modulation,neuroprotection,and inflammation regulation.However,challenges such as poor bioavailability and safety concerns remain significant hurdles to overcome.The interactions between curcumin and the gut microbiome hold promise for therapeutic interventions in a diverse range of health conditions.Further research is needed to optimize curcumin formulations,improve bioavailability,and address safety concerns.
文摘BACKGROUND Current osteoarthritis(OA)treatments focus on symptom relief without addressing the underlying disease process.In regenerative medicine,current treatments have limitations.In regenerative medicine,more research is needed for intra-articular stromal vascular fraction(SVF)injections in OA,including dosage optimization,long-term efficacy,safety,comparisons with other treatments,and mechanism exploration.AIM To compare the efficacy of intra-articular SVF with corticosteroid(ICS)injections in patients with primary knee OA.METHODS The study included 50 patients with Kellgren-Lawrence grades II and III OA.Patients were randomly assigned(1:1)to receive either a single intra-articular SVF injection(group A)or a single intra-articular ICS(triamcinolone)(group B)injection.Patients were followed up at 1,3,6,12,and 24 months.Visual analog score(VAS)and International Knee Documentation Committee(IKDC)scores were administered before the procedure and at all followups.The safety of SVF in terms of adverse and severe adverse events was recorded.Statistical analysis was performed with SPSS Version 26.0,IBM Corp,Chicago,IL,United States.RESULTS Both groups had similar demographics and baseline clinical characteristics.Follow-up showed minor patient loss,resulting in 23 and 24 in groups A and B respectively.Group A experienced a notable reduction in pain,with VAS scores decreasing from 7.7 to 2.4 over 24 months,compared to a minor reduction from 7.8 to 6.2 in Group B.This difference in pain reduction in group A was statistically significant from the third month onwards.Additionally,Group A showed significant improvements in knee functionality,with IKDC scores rising from 33.4 to 83.10,whereas Group B saw a modest increase from 36.7 to 45.16.The improvement in Group A was statistically significant from 6 months and maintained through 24 months.CONCLUSION Our study demonstrated that intra-articular administration of SVF can lead to reduced pain and improved knee function in patients with primary knee OA.More adequately powered,multi-center,double-blinded,randomised clinical trials with longer follow-ups are needed to further establish safety and justify its clinical use.
文摘During the 2nd Indochina War which started in 1959, the United States Central Intelligence Agency (CIA), Air America, and the Air Force waged a secret and unconventional air war in Laos from Udorn Air Force base located in Thailand and across the Mekong River from Vientiane, Laos. Starting in 1961, four years before the official start of the American-Vietnam War, Agent Blue, the arsenic-based herbicide used to kill rice and other food crops, was used extensively in Laos, Vietnam and to a lesser extent in Cambodia. During the secret 2nd Indochina War and the Vietnam Civil War the public knew little about the use of Agent Blue. After the official start of the American-Vietnam War in 1965, the United States media news reports, about chemical warfare were dominated by the story of Agent Orange and its devastating impacts. The public knew very little about the previous use of Agent Blue in both wars. The first known media pick up of the Agent Blue (arsenic based) and Agent Pink, Agent Green, and Agent Purple (all three contain 2, 4, 5-T and unknown amounts of dioxin TCDD) was in May of 1964. Jim G. Lucas, a Scripps-Howard staff reporter submitted an article that was published as an editorial in Washington Post on May 26, 1964. The next news reference to this chemical weapon was a Letter to the Editor published in the New York Times titled “Agent Blue” in Vietnam by Arthur H. Westing in (1971). The use of herbicides, including Agent Blue in Laos during the 2nd Indochina War, was kept a secret until 1982, when a draft of Buckingham’s study of Operation Ranch Hand was made public. Much about the U.S. war effort in Laos is still classified. In a 2014 issue of the VVA Veteran magazine, Loana Hoylman published an article on “Today’s Blue Arsenic in the Environment”. The first refereed journal article on this topic, “The Fate of Agent Blue, the Arsenic-Based Herbicide, Used in South Vietnam during the Vietnam War” was published in 2020 in the Open Journal of Soil Science by Kenneth R. Olson and Larry Cihacek. In 2021 the Asia Times (print) and VietnamVeteranNews (radio podcast) picked up the Agent Blue story. During the early 2020s, Olson published six additional refereed journal articles on Agent Blue, cacodylic acid, and arsenic. The primary objective is to determine why no major news organization in the United States, including the New York Times and Washington Post, have never investigated Agent Blue use during the 2nd Indochina and Vietnam wars? Why did the use of Agent Blue story, used to destroy Laotian and South Vietnamese civilian food (rice) sources and production sites, received only very limited coverage by US print media news organizations during the last 64 years?
文摘BACKGROUND Adipose-derived stem cells(ADSCs)and the stromal vascular fraction(SVF)have garnered substantial interest in regenerative medicine due to their potential to treat a wide range of conditions.Traditional enzymatic methods for isolating these cells face challenges such as high costs,lengthy processing time,and regulatory complexities.AIM This systematic review aimed to assess the efficacy and practicality of nonenzymatic,mechanical methods for isolating SVF and ADSCs,comparing these to conventional enzymatic approaches.METHODS Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines,a comprehensive literature search was conducted across multiple databases.Studies were selected based on inclusion criteria focused on non-enzymatic isolation methods for SVF and ADSCs from adipose tissue.The risk of bias was assessed,and a qualitative synthesis of findings was performed due to the methodological heterogeneity of the included studies.RESULTS Nineteen studies met the inclusion criteria,highlighting various mechanical techniques such as centrifugation,vortexing,and ultrasonic cavitation.The review identified significant variability in cell yield and viability,and the integrity of isolated cells across different non-enzymatic methods compared to enzymatic procedures.Despite some advantages of mechanical methods,including reduced processing time and avoidance of enzymatic reagents,the evidence suggests a need for optimization to match the cell quality and therapeutic efficacy achievable with enzymatic isolation.CONCLUSION Non-enzymatic,mechanical methods offer a promising alternative to enzymatic isolation of SVF and ADSCs,potentially simplifying the isolation process and reducing regulatory hurdles.However,further research is necessary to standardize these techniques and ensure consistent,high-quality cell yields for clinical applications.The development of efficient,safe,and reproducible non-enzymatic isolation methods could significantly advance the field of regenerative medicine.
基金Grants from Key Research and Development Projects of the Ministry of Science and Technology Funding Support:Standards for Traditional Chinese Medicine Technology(No.2019YFC1712003)2023 First-class Discipline Construction Project of Beijing University of Chinese Medicine:Tuina。
文摘OBJECTIVE:To establish a standardized framework encompassing the precise locations,manipulations,functions and indications of specific acupoints in the field of paediatric Tuina.METHODS:The development of consensus involved three distinct stages.Initially,a list of paediatric Tuina specific acupoints was compiled based on an extensive literature review,which was subsequently supplemented through expert interviews.In the second stage,the Delphi method was employed to assess the significance of acupoint locations,manipulations,functions,and indications.In situations where the questionnaire survey failed to yield agreement or when the experts held reservations,the nominal group approach was utilized during the expert consensus meeting.The final version of the technical standardized material was ultimately determined during an expert consensus conference.After undergoing external peer review and evaluation,the completed draft was prepared for public dissemination RESULTS:The comprehensive list identified a total of 66 specific acupoints.The location and manipulation questionnaire consisted of 156 items based on the literature database,while the function and indication questionnaire contained 116 items.Two rounds of Delphi surveys were conducted for the location and manipulation category,and another two rounds of Delphi surveys were conducted for the function and indication category.During the experts consensus meeting The panel of experts conducted in-depth discussions on 61 questions,resulting in the formulation of technical guidelines for the locations,manipulations,functions,and indications of 64 paediatric Tuina acupoints.Subsequently,the research team compiled and edited the draft of the technical guidelines for acupoints of paediatric Tuina,which was finalized after external review and feedback.CONCLUSION:This study successfully established the recognized technical standards for practitioners of paediatric Tuina,thereby standardizing clinical practices and providing a foundation setting the framework for future research.The guidelines offer theoretical insights and recommendations for conducting clinical studies comparing different acupoint sites,as well as modifying or enhancing treatment regimens.
